Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-23T12:20:30Z No. of bitstreams: 1 2011 - Kelly Christane S do Nascimento.pdf: 1254140 bytes, checksum: 1bc209e640d2d51ff8e4941920868826 (MD5) / Made available in DSpace on 2016-08-23T12:20:30Z (GMT). No. of bitstreams: 1 2011 - Kelly Christane S do Nascimento.pdf: 1254140 bytes, checksum: 1bc209e640d2d51ff8e4941920868826 (MD5) Previous issue date: 2011-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The prevalence of mellitus diabetes comes growing with the advance of the technology varying between 7% and 13.4% in different age groups and in some states of Brazil. To improve the patient quality of life it is necessary to replace these foods for others, with acceptable sensorial characteristics and that not contain high-grade of carbohydrates or low glicemic index. The current work evaluated the glicemia of normal and diabetic rats that weigh between 242,0g and 378,4g induced for the saline solution of aloxana that had received ad libitum passion-fruit juice (Passiflora edulis) sweeten with monossodic saccharin and cyclamate sodium e commercial sucrose add with 4 differents type of fibers with totally 8 types of juice?s and 16 groups and fed with rat?s ration. The experiment lasted 21 days where the variations of weights and the glicemias of each animal had been verified for 7 days, being collected a samples: T1, T2, T3. The results had shown that the passion-fruit juice with hypocalorific edulcorante had hypoglycemic effect in healthy rats during the experiment and in diabetic animal in the same period. Almost showed a hyperglycemic effect in goma guar?s edulcorante control group and in goma xantana?s While the juice with sugar and edulcorante control group increased the glucose in both groups. We conclude that the juice sweetened with hypocalorifics edulcorantes in healthy rats can damage the healthy population?s healthy, becoming the guideline for the greaters researches in this field. / A preval?ncia do diabetes mellitus vem crescendo com o avan?o da tecnologia e da modernidade, variando entre 7 % a 13, 4% em diferentes faixas et?rias e determinados estados no Brasil. Para melhorar a qualidade de vida do diab?tico, se faz necess?ria, substituir esses alimentos por outros que contenha reduzido teor de carboidratos ou baixo ?ndice glic?mico e com caracter?sticas sensoriais aceit?veis. O presente trabalho avaliou a glicemia de ratos normais e diab?ticos pesando em m?dia 279,3 g ? 36,7 induzidos pela solu??o salina de aloxana e que receberam ra??o comercial para ratos e refresco de maracuj? ad libitum, sendo que para um grupo a bebida foi ado?ada com sacarose comercial e para o outro com sacarina monoss?dica + ciclamato de s?dio ambos adicionados de 4 fibras sol?veis diferentes totalizando 8 refrescos e 16 grupos; Durante 21 dias foram verificadas em cada animal as varia??es de pesos e das glicemias, no tempo zero (T0) e a cada 7 dias, sendo realizadas 3 coletas T1, T2 e T3. Os resultados mostraram que o refresco de maracuj? com edulcorante hipocal?rico teve efeito hipoglic?mico nos ratos normais e nos animais diab?ticos no mesmo per?odo e efeito hiperglic?mico nos grupos Goma Guar com edulcorante e na Goma Xantana provocou aumento da glicemia nos dois grupos, levando a morte de 20,8% das cobaias diab?ticas. Esses resultados permitem concluir que o refresco ado?ado com sacarina monoss?dica + ciclamato de s?dio administrados ? ratos cobaias normais prejudicial a sa?de dos animais doentes, tornando-se pauta para maiores pesquisas nesse campo.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-23T13:52:58Z No. of bitstreams: 1 2011 - Rubenval Francisco de Jesus Feitosa.pdf: 13455824 bytes, checksum: 95b1e24703c5e57f468f589e100bfb62 (MD5) / Made available in DSpace on 2016-08-23T13:52:58Z (GMT). No. of bitstreams: 1 2011 - Rubenval Francisco de Jesus Feitosa.pdf: 13455824 bytes, checksum: 95b1e24703c5e57f468f589e100bfb62 (MD5) Previous issue date: 2011-09-19 / This study aimed to analyze the perception of agroecology students about the use of teaching practices in animal production. The IF - Campus St. Kitts was the setting for the research with the participation of students in the third series of the Technical Course for Integrated Agricultural Secondary School, in 2010, in an interdisciplinary way, democratic and dialogical, since so- professionals is to interfere in family agriculture and other sectors of agricultural production, disseminating new technologies to meet the needs of the present generation without compromising the future. In this context, the relationship between education and work, the motivational factors for the learning process, understanding and transformation of educational practice and the concepts, principles and prospects of agroecology, rural extension, agro-ecological and sustainable family farms as a reference gives theoretical support to this research. We applied the methodology and the qualitativequantitative descriptive method of data collection took place by direct observation of phenomena with the help of literature search, semi-structured forms of employment, teaching practices, lectures, semi-structured questionnaires, seminars and technical visits. In the first stage of the research project was presented to students looking to motivate them and then applied the questionnaire (attached to), to determine the profile of students. In the second stage 60 questionnaires were collected for data analysis, followed by the selection and preparation of implementation schedule of teaching practices used in animal production of relevance to learning. There was the holding of lectures, seminars, technical visits. In the third step, teaching practices were given to students in the Educational Curriculum Unit - Cattle. In the fourth stage 60 questionnaires were applied in order to complete the survey of agroecological awareness of the students, the pedagogical practices employed in animal production. The results are used for reflection, will face disconnection between the student, school and family, inefficient management, the man / nature and production with sustainability, compromising the quality of education. The results in terms of level of awareness of students on the issues already mentioned are suggestive that this work can be used as input for future redirects the politics employed in the educational process of the Campus Saint Kitts, aimed at student success / Este estudo teve como objetivo analisar a percep??o agroecol?gica dos alunos, quanto ao emprego das pr?ticas pedag?gicas na produ??o animal. O IFSE - Campus S?o Crist?v?o foi o cen?rio para a realiza??o da pesquisa com a participa??o dos alunos das terceiras s?ries do Curso T?cnico Integrado ao Ensino M?dio em Agropecu?ria, no ano de 2010, de forma interdisciplinar, democr?tica e dial?gica, uma vez que se formam profissionais para interferir na agricultura familiar e demais segmentos da produ??o agropecu?ria, disseminando novas tecnologias capazes de atender ?s necessidades da gera??o presente sem comprometer a futura. Neste contexto, a rela??o educa??o e trabalho; os fatores motivacionais para o processo ensino aprendizagem; a compreens?o e transforma??o da pr?tica educativa; os conceitos, princ?pios e perspectivas da agroecologia; a extens?o rural: desenvolvimento sustent?vel agroecol?gico e a agricultura familiar constituem-se como referencial te?rico que d? sustenta??o a esta pesquisa. Empregou-se a metodologia qualiquantitativa descritiva e o m?todo de coleta de dados se deu pela observa??o direta dos fen?menos com aux?lio de pesquisa bibliogr?fica, entrevista semi-estruturada, emprego de formul?rios, pr?ticas pedag?gicas, aulas te?ricas, question?rios semi-estruturados, semin?rios e visitas t?cnicas. Na primeira etapa da pesquisa, foi apresentado o projeto aos alunos procurando motiv?-los e em seguida aplicou-se o question?rio (anexo a), visando conhecer o perfil dos alunos. Na segunda etapa foram coletados 60 question?rios para an?lise dos dados, seguido da sele??o e elabora??o do cronograma de execu??o das pr?ticas pedag?gicas empregadas na produ??o animal de relev?ncia para o aprendizado. Verificou-se a realiza??o de aulas te?ricas, semin?rios, visitas t?cnicas. Na terceira etapa, foram ministradas as pr?ticas pedag?gicas para os alunos na Unidade Did?tica Pedag?gica ? Bovinocultura. Na quarta etapa foram aplicados 60 question?rios com o prop?sito de concluir o levantamento da percep??o agroecol?gica dos alunos, quanto ?s pr?ticas pedag?gicas empregadas na produ??o animal. Os resultados servem para reflex?es, face ? desarticula??o entre o aluno, a escola e a fam?lia, gest?o pouco eficiente, rela??o homem/natureza e a produ??o com sustentabilidade, comprometendo a qualidade do ensino. Os resultados em termos de n?vel de percep??o dos alunos, quanto ?s quest?es j? mencionadas, s?o sugestivos de que este trabalho pode servir de subs?dios para futuros redirecionamentos das pol?ticas empregadas no processo educativo do Campus S?o Crist?v?o, visando o sucesso do aluno

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-23T17:05:27Z No. of bitstreams: 1 2011 - Michele Valadares Deveza.pdf: 2385296 bytes, checksum: 127bd8c33d0595f06af98fe22cfc21e1 (MD5) / Made available in DSpace on 2016-08-23T17:05:27Z (GMT). No. of bitstreams: 1 2011 - Michele Valadares Deveza.pdf: 2385296 bytes, checksum: 127bd8c33d0595f06af98fe22cfc21e1 (MD5) Previous issue date: 2011-02-08 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The bee?s pollen is a product rich in essential nutrients to feed the bees. In the hive it goes through chemical processes and becomes the bee?s bread, of extreme importance for the growth of larvae. Currently, the use of pollen in the human?s cuisine has been increasing for its therapeutic properties, and by its use in the food industry. To produce quality pollen the hive needs to be healthy, and bees, like any other animal, are susceptible to various diseases, parasites and predators, which can affect their development and productivity. The The Brazilian Sac Brood (BSB) is one of these diseases, which in Brazil affects honeybee larvae. In apiaries of Rio de Janeiro state, a high mortality of larvae with symptoms similar to the BSB is occurring, and until now, there is no definite conclusion regarding the etiologic agent of this disease in the state. Therefore, the objectives of this study was to clarify two issues: 1) provide a mycological research, through a study case, of bees? bread samples, adult bees and bees? pollen in areas affected and not affected by the BSB, aiming the elucidation of its etiologic agent, 2) verify the hygienic quality of dehydrated pollen samples sold in Rio de Janeiro state. A total of 48 samples of bees? bread, 15 of adult bees and 12 of pollen were collected from endemic regions of the BSB. The samples were collected in the months before, during and after the period of the disease. The dehydrated pollen samples were also purchased in commercial stores of the state, totaling 27 samples. Fungal species enumeration (cfu g-1) was through decimal dilution method using general culture media and selective ones. The isolation frequency and relative density of species were determined. The toxigenic profile of strains was determined, as also the detection and quantification of aflatoxin B1 in bread and bees? pollen. The results obtained from the study case samples showed that the bees? bread was the best vehicle of contamination for the hive. It was also observed a high fungal counts and the presence of mycotoxins and entomopathogenic fungi such as Aspergillus flavus, A. niger aggregate and A. fumigatus, which cause a decrease of the immune response, leaving them susceptible to various diseases, including the BSB itself. Pollen dehydrated samples showed a high fungal contamination with 92% of the samples in unsatisfactory hygienic conditions according to legal standards. A total of 25% of the A. flavus strains showed to be aflatoxigenic. The high percentage of unhygienic samples combined with the presence of a large variety of fungal contaminants, including mycotoxin-producing species, evidences that the legislation needs to be updated so that it can be safely commercialized. It is considered that the whole line production of bees? pollen, since its extraction until its storage should be monitored in order to minimize risks to human health. / O p?len ? um produto ap?cola rico em nutrientes essenciais para a alimenta??o das abelhas. Na colmeia ele passa por processos qu?micos e se torna o p?o de abelhas, de extrema import?ncia para o crescimento das larvas. Atualmente, o p?len ap?cola vem se destacando tamb?m na alimenta??o humana, tanto por suas propriedades terap?uticas, quanto pela sua utiliza??o na ind?stria aliment?cia. Para a produ??o de um p?len de qualidade a colmeia precisa estar sadia, e as abelhas, como qualquer outro animal, s?o suscept?veis a v?rias doen?as, parasitas e predadores, que podem prejudicar seu desenvolvimento e produtividade. A Cria Ensacada Brasileira (CEB) ? uma dessas doen?as, que no Brasil acomete larvas de abelhas mel?feras. Em api?rios do estado do Rio de Janeiro v?m ocorrendo uma alta mortalidade de larvas com sintomatologia semelhante a da CEB, e ainda n?o h? uma conclus?o definitiva acerca do agente etiol?gico desta doen?a no estado. Portanto, os objetivos desse estudo visam esclarecer duas problem?ticas: 1) avaliar, atrav?s de estudo de caso, amostras de p?o de abelhas, abelhas adultas e p?len ap?cola em regi?es atingidas e n?o atingidas pela CEB, diagnosticando a presen?a de fungos, visando fornecer informa??es importantes para a elucida??o do agente etiol?gico da CEB no estado do Rio de Janeiro; 2) verificar a qualidade higi?nica das amostras de p?len desidratado comercializado no estado do Rio de Janeiro. Um total de 48 amostras de p?o de abelhas, 15 de abelhas adultas e 12 de p?len ap?cola, foram adquiridas de regi?es end?micas da CEB. As coletas ocorreram nos meses que antecederam, durante e ap?s o per?odo da doen?a. J? as amostras de p?len desidratado foram adquiridas no com?rcio do estado, totalizando 27 amostras. A enumera??o da micobiota (ufc g-1) foi realizada atrav?s do m?todo de dilui??o decimal com semeadura em placas utilizando-se meios de cultivo gerais e seletivos. Foram determinadas a freq??ncia de isolamento dos g?neros f?ngicos e a densidade relativa das esp?cies. Foi determinado o perfil tox?geno de cepas isoladas e foi feita a detec??o e quantifica??o de aflatoxina B1 no p?o de abelhas e p?len ap?cola. Nos resultados obtidos das amostras do estudo de caso, o p?o de abelhas mostrou ser o melhor substrato para o ve?culo de contamina??o para a colmeia. Observou-se tamb?m altas contagens f?ngicas, al?m da presen?a de micotoxinas e fungos entomopatog?nicos, como Aspergillus flavus, A. niger agregados e A. fumigatus o que favorece a queda da imunidade desses insetos deixando-os suscet?veis a diversas doen?as, entre elas a pr?pria CEB. Em rela??o ao p?len desidratado, os resultados mostraram uma alta carga f?ngica com 92% das amostras em condi??es higi?nicas insatisfat?rias segundo os padr?es legais consultados e 25% das cepas de A. flavus mostraram-se potencialmente produtoras de aflatoxinas. Os alt?ssimos percentuais de reprova??o das amostras para a qualidade higi?nica, aliado ? presen?a de grande variedade de fungos contaminantes, incluindo esp?cies produtoras de micotoxinas, ? uma evid?ncia de que a legisla??o necessita ser atualizada, para que este produto possa ser comercializado com seguran?a. Considera-se que toda a linha de produ??o do p?len ap?cola, desde sua coleta at? o armazenamento deva ser monitorada, a fim de minimizar os riscos para a sa?de humana

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-24T14:57:04Z No. of bitstreams: 1 2011 - Denise Bilibio.pdf: 1776424 bytes, checksum: 7a2458925cc528e896bf5d4baac6f571 (MD5) / Made available in DSpace on 2016-08-24T14:57:04Z (GMT). No. of bitstreams: 1 2011 - Denise Bilibio.pdf: 1776424 bytes, checksum: 7a2458925cc528e896bf5d4baac6f571 (MD5) Previous issue date: 2011-09-26 / Basic education has a strategic role in building a nation, and vocational teaching needs to articulate culture, knowledge, technology and work for the training of professionals prepared for new challenges in a globalized and competitive world. This way, the teaching-learning process search for methodological alternatives that make the process more dynamic and flexible. Then, it was used Interactive Methodology to promote the construction of knowledge in the process of teaching-learning chemistry applied to the technical course in agribusiness. The used theoretical contributions to this work was of Dialectical-Hermeneutical Circle (CHD) of Guba; Lincoln (1989) with adjustments and the Hermeneutic Analysis-Dialectics (AHD) of Minayo (1996). The survey was developed in Campus IFRS ? Erechim, with two classes of the technical course in agribusiness, subsequent mode. For the application of CHD, the actors of the process need to be familiar with the subject to practice the dialogue and knowledge building, as well as the use of small groups also provide the dialogue. In this research, it was used together the CHD and projective methods of interview, allowing the methodology? structure, data collection and monitoring the processes of knowledge?s construction of students through the establishment of dialogue from their own experiences. The results achieved in the survey showed that chemistry is still taught in the traditional way that aims to memorization; the use of Interactive Methodology is an innovative tool, that could be able to be used in technical education in agribusiness, especially teaching applied chemistry, forming critic professionals / A educa??o b?sica possui papel estrat?gico na constru??o de uma na??o e o ensino profissionalizante precisa articular cultura, conhecimento, tecnologia e trabalho para a forma??o de profissionais preparados para novos desafios em um mundo globalizado e competitivo. Desta forma, o processo ensino-aprendizagem precisa buscar alternativas metodol?gicas capazes de tornar o processo mais din?mico e vers?til. A Metodologia Interativa consiste numa alternativa para promover a constru??o do conhecimento no processo de ensino aprendizagem de qu?mica aplicada ao curso t?cnico em agroind?stria, tendo como aportes te?ricos para este trabalho a utiliza??o do Circulo hermen?utico-dial?tico (CHD) de Guba; Lincoln (1989) com adapta??es e a An?lise hermen?utica-dial?tica (AHD) de Minayo (1996). A pesquisa foi desenvolvida no IFRS ? Campus Erechim, com duas turmas do Curso T?cnico em Agroind?stria, modalidade subseq?ente. Observou-se que para a aplica??o do CHD, os atores do processo precisam estar familiarizados com o assunto para a efetiva??o do di?logo e constru??o do conhecimento, assim como a utiliza??o de pequenos grupos tamb?m propiciam o di?logo. A utiliza??o conjunta, nesta pesquisa, do CHD e de m?todos projetivos de entrevista permitiu a estrutura??o da metodologia a coleta de dados e o acompanhamento dos processos de constru??o do conhecimento dos alunos atrav?s do estabelecimento do di?logo a partir de suas experi?ncias. Os resultados alcan?ados na pesquisa demonstraram que a disciplina de qu?mica, ainda ? trabalhada dentro de um ensino tradicional que visa ? memoriza??o, e que a utiliza??o da Metodologia Interativa constitui-se em uma ferramenta inovadora, capaz de ser utilizada no ensino t?cnico em agroind?stria, em especial no ensino de qu?mica aplicada contribuindo para a forma??o profissionais cr?ticos.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-24T17:14:43Z No. of bitstreams: 1 2011 - Ellen Aparecida Nogueira Pereira.pdf: 1048943 bytes, checksum: e1f0b190db0be89726ed357fa5a6127e (MD5) / Made available in DSpace on 2016-08-24T17:14:43Z (GMT). No. of bitstreams: 1 2011 - Ellen Aparecida Nogueira Pereira.pdf: 1048943 bytes, checksum: e1f0b190db0be89726ed357fa5a6127e (MD5) Previous issue date: 2011-08-29 / The objective of this work was search more information about insect that promove damaged in forest species seeds, studing especially the insects damage. Seeds samples used to make biojewellery was collected and take to Forest Entomology Laboratory at Rural University of Rio de Janeiro, where the damages were observed and the family of the insects were identified. The families were: Scolitydae and Anobiidae. It is possible to decrease the insect damage with appropriate seed store or make use of damage seeds, after heating with the objective of eliminate the insects. To know the viability of seed collection, Leguminosae seeds, used in urban forestry, Albizzia lebbeck (L) Bentham, Cassia fistula L., was collected in Serop?dica (RJ), one sample with 500 fruits of Albizzia lebbeck (L.) Bentham, after processing, were found: 23,38% of the seeds were damaged by insects, 60,41% of the seeds were empty and 16,21% were not damaged by insects. The sample with 100 fruits of Cassia fistula L. was collected in Itagua? (RJ) and 83,35% of the seeds were damaged by insects, 10,96% of the seeds were empty and 5,69% were not damaged by insects. The seeds of both species are damaged by Bruchidae. To check the damage caused by insects Anobiidae in the storage, they were placed in contact with healthy seeds and seeds damaged by insects of Albizzia lebbeck (L.) Bentham and seeds of healthy and mechanically damaged of Adenanthera pavonina L. Twenty adult insects were used and 10 seeds for each situation, using five repetitions. After 60 days there was Anobiidae damage just in damaged seeds / O objetivo desse trabalho foi buscar maiores informa??es sobre danos de insetos em sementes de esp?cies florestais utilizadas para a confec??o de bioj?ias. Amostras de sementes utilizadas para confec??o de bioj?ias de um projeto foram coletadas e levadas ao laborat?rio de Entomologia Florestal da Universidade Federal Rural do Rio de Janeiro, onde se realizou a an?lise dos danos e a identifica??o da fam?lia dos insetos. Foram encontradas duas fam?lias causando danos nas sementes: Scolytidae e Anobiidae. Visando o aproveitamento das sementes atacadas, as mesmas foram tratadas e posteriormente utilizadas para a confec??o de bioj?ias e artesanato. O estudo mostrou que ? poss?vel aumentar o rendimento das sementes, seja com o armazenamento adequado ou at? mesmo aproveitando as sementes atacadas, utilizando o aquecimento para eliminar os insetos. Para conhecer a viabilidade da coleta de leguminosas ex?ticas utilizadas na arboriza??o, Albizzia lebbeck (L) Bentham e Cassia fistula L., coletou-se em Serop?dica (RJ) uma amostra de 500 frutos de Albizzia lebbeck (L.) Bentham, ap?s beneficiamento foram registradas 23,38% de sementes danificadas por insetos, 60,41% de sementes chochas e 16,21% de sementes sadias. A amostra de 100 frutos de Cassia fistula L. foi coletada em Itagua? (RJ) e foram registradas 83,35% de sementes danificadas por insetos, 10,96% de sementes chochas e 5,69% de sementes sadias. As sementes de ambas as esp?cies s?o danificadas por insetos da fam?lia Bruchidae. Para verificar os danos causados por insetos da fam?lia Anobiidae no armazenamento, os mesmos foram colocados em contato com sementes sadias e danificadas por insetos de Albizzia lebbeck (L.) Bentham e sementes sadias e danificadas mecanicamente de Adenanthera pavonina L. Foram utilizados vinte insetos adultos e 10 sementes para cada situa??o, sendo cinco repeti??es. Depois de 60 dias verificou-se dano de Anobiidae somente nas sementes danificadas.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-30T14:26:59Z No. of bitstreams: 1 2010 - Fernanda Peixoto Giori.pdf: 2362278 bytes, checksum: a15d423533272c981b15d7f22b714be1 (MD5) / Made available in DSpace on 2016-08-30T14:26:59Z (GMT). No. of bitstreams: 1 2010 - Fernanda Peixoto Giori.pdf: 2362278 bytes, checksum: a15d423533272c981b15d7f22b714be1 (MD5) Previous issue date: 2010-02-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior-CAPES / Brazil was adverted by the World Health Organization (WHO) as a sub-clinical area of serious vitamin A deficiency. Thus, the Brazilian Agricultural Research Corporation (EMBRAPA) is selecting and improving varieties of sweet potatoes with higher levels of Beta-carotene, pro-vitamin A. The carotenoids provitamin A amount of in foods does not necessarily correspond the amount that is absorbed and metabolized by the body. For a better determination of these values and knowledge of the mechanisms of its transport and absorption, it is necessary to understand the factors that lead to the food matrix release, until the absorption and the influence on the promotion and maintenance in human health. In order to perform preliminary studies of its absorption, this study aims to determine the efficiency of micellization of Beta -carotene in orange sweet potato (Ipomoea batatas, Lam), by applying a in vitro digestion as a tool for determining the bioaccessibility, which is the first step for bioavailability determination. This approach aims to simulate the oral, gastric and intestinal stages of human. The digestion was performed with 10 g of fresh samples, homozeneided with 5% (w/w) of canola oil. The extraction was performed with acetone and petroleum ether and the micellar fraction with petroleum ether, NaCl 10% (w / v) and NaSO4 2% (w/v) and involves the use of enzymes as -amylase , pepsin, bile, pancreatin, lipase and mucin, and inorganic compounds such as KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. The physiological variations are reproduced by the heating bath shaker with orbital gyrus (37?C) and centrifugation (5000g, 45 min). Quantification and determination of the profile of carotenoids were performed by high performance liquid chromatography (HPLC) with YCM ? C30 Carotenoid S-3 4.6 x 250mm column. Quantification of total carotenoids was performed by UV-VIs. The whole procedure was performed under controlled temperature (25 ? C) and light. The Beta -carotene was present mainly with levels of 86%, 73% and 82% for access 1, 2 and 3 and after digestion, the profile of Beta -carotene has set levels of 96%, 89% and 100%, respectively. The efficiency of micellization was 23.8%, 28% and 25% for 1.2 and 3 hits, indicating Beta -carotene transfer of the food matrix to micelles, corresponding to bioaccessibility of the compound. This methodology proved to be faster and cheaper, since the in vivo studies are costly, complex and require more time. / O Brasil foi classificado pela Organiza??o Mundial da Sa?de (OMS) como ?rea de car?ncia sub-cl?nica grave de vitamina A. Assim, a Empresa Brasileira de Pesquisa Agropecu?ria (EMBRAPA) v?m selecionando e melhorando variedades de batata-doce com teores maiores de Beta-caroteno, composto pr?-vitamina A. A quantidade de caroten?ides pr?-vitamina A presentes nos alimentos n?o corresponde necessariamente ?quela quantidade absorvida e metabolizada pelo organismo. Para uma melhor determina??o destes valores e conhecimento dos mecanismos de transporte e absor??o deste composto, faz-se necess?rio, o entendimento dos fatores que levam ? sua libera??o da matriz do alimento, at? a extens?o de sua absor??o, bem como a influ?ncia na promo??o e manuten??o da sa?de humana. A fim de realizar estudos preliminares de sua absor??o, este trabalho visa determinar a efici?ncia de miceliza??o de Beta-caroteno de batata-doce de polpa alaranjada (Ipomoea batatas, Lam.), atrav?s da aplica??o de digest?o in vitro, como ferramenta de determina??o da bioacessibilidade, etapa preliminar para a determina??o da biodisponibilidade. Esta metodologia visa simular as etapas de digest?o oral, g?strica e intestinal humana. Foram pesados 10g de amostra in natura e adicionados 5% (p/p) de ?leo de canola. A extra??o do alimento foi realizada com acetona e ?ter de petr?leo e a da fra??o micelar, com ?ter de petr?leo, NaCl 10%(p/v) e NaSO4 2%(p/v) . Enzimas como: a-amilase, pepsina, bile, pancreatina, lipase e mucina, bem como compostos inorg?nicos, tais como KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. As varia??es fisiol?gicas foram reproduzidas pelo banho de aquecimento com giro orbital (37?C) e centrifuga??o (5000g;45 min). A quantifica??o e determina??o do perfil de caroten?ides foi realizada por cromatografia l?quida de alta efici?ncia (CLAE), com coluna YCM? Carotenoid C30 S-3 de 4,6 x 250mm. A quantifica??o de caroten?ides totais foi realizada por espectrofotometria UV-VIs. Todo o procedimento foi executado sob temperatura (25?C) e luz controlada. O Beta-caroteno estava presente majoritariamente, com teores de 86%, 73% e 82%, para as variedades 1, 2 e 3 e ap?s a digest?o, o perfil do Beta-caroteno passou a configurar teores de 96%, 89% e 100%, respectivamente. A efici?ncia de miceliza??o foi de 23,8%, 28% e 28,9% para as variedades 1,2 e 3, indicando a transfer?ncia do - caroteno da matriz do alimento para as micelas, correspondendo a bioacessibilidade deste composto. Esta metodologia demonstrou-se mais r?pida e mais barata, quando comparada aos estudos in vivo, que s?o mais onerosos, complexos e demandam mais tempo.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-08T12:13:31Z No. of bitstreams: 1 2011 - Jos? Antonio Batista da Silva.pdf: 1901739 bytes, checksum: bf67996decc8c7cef031a8ce05d6f464 (MD5) / Made available in DSpace on 2016-09-08T12:13:31Z (GMT). No. of bitstreams: 1 2011 - Jos? Antonio Batista da Silva.pdf: 1901739 bytes, checksum: bf67996decc8c7cef031a8ce05d6f464 (MD5) Previous issue date: 2011-12-08 / This work was carried out from August 2007 to July 2011, in Itabora?, RJ, Brazil, and aimed to identify the Calliphoridae species exist in a mangrove swamp, verifying which are the predominant species, highlighting the proportions of males and females, and also analyzing the influence of abiotic factors such as temperature, humidity and moon phases, and also providing an analysis of the abundance, richness, diversity and similarity between the periods of sampling, ecological relationships between species of the same family and the ecological relationship between Calliphoridae and Phoridae parasitoids in Guapi-Mirim Environmental Protection Area, (Guapi-Mirim EPA) in the same city. Semiweekly collections were carried out over 48 months (96 collections). The traps were suspended at a height of 1.20 m above the ground for a period of 48 hours for each collection. To trap and collect the flies, four plastic traps (35 cm x 15 cm) were placed 100m apart each other in the studied area. The bait used was based on fish (sardine) in decomposition. The insects caught were killed by asphyxiation with 70% ethanol inside the trap. After asphyxiation all specimens were put into plastic pots containing 70% ethanol. The specimens were taken to the Laborat?rio de Transmissores de Leishmanioses (Setor de Entomologia M?dica e Forense) - IOC/FIOCRUZ, RJ, where they were separated by the collection day, counted and then identified using a stereoscopic microscope and the dichotomous keys for families and species. The abundance of Calliphoridae flies was statistically analyzed using the Statistica 7.1 program (StatSoft 2005) for the non-parametric test Kruskal-Wallis one way ANOVA (p< 0.05). Also the Kendall Tau Correlation test, Mann-Whitney (significance of 95%; p<0.05) and chi-square test (?2) were used to analyze. The Shannon-Wiener diversity index, Bray-Curtis similarity and Euclidean Distance were used. A total of 4,531flies were collected. These flies belonged to ten (10) species of the Calliphoridae family: Chrysomya megacephala (Fabricius, 1794) (86.40%), Chrysomya albiceps (Wiedemann,1819) (5.72%), Cochliomyia macellaria (Fabricius, 1775) (4.94%), Chrysomya putoria (Wiedemann, 1818) (2.10%), Lucilia eximia (Wiedemann, 1819) (0.30%), Cochliomyia hominivorax (Cocquerel, 1858) (0.22%), Chloroprocta idioidea (Robineau-Desvoidy, 1930) (0.20%), Hemilucilia segmentaria (Fabricius, 1805) (0.04%), Hemilucilia semidiaphana (Rondani, 1850) (0.04%), Lucilia cuprina (Wiedemann,1830) (0.04%). The species C. megacephala was the one that presented the greatest abundance. Spring was the preferred season for all species; temperatures between 30.5 and 32.40C and relative humidity between 56.8 and 61.7% were considered those where there was a frenzy of oviposition; the full and new moons were the phases during which there was a higher occurrence of flies; Megaselia scalaris occurred more frequently in the most abundant Calliphoridae species in the same season of the year thus allowing greater dispersion of eggs; Calliphoridae species had a higher rate survival with increased food resources resulting from the polluting activity / Este trabalho foi realizado entre agosto de 2007 e julho de 2011, no Munic?pio de Itabora?, RJ, Brasil, e teve como objetivo identificar as esp?cies de Calliphoridae existentes em uma ?rea de mangue, quantificar as predominantes, destacando as propor??es de machos e f?meas, e tamb?m analisar a influ?ncia de fatores abi?ticos, tais como temperatura, umidade do ar e fases lunares; e ainda relacionar a abund?ncia, a riqueza, a diversidade e a similaridade entre os per?odos de coletas, rela??es ecol?gicas entre as esp?cies da entomofauna da mesma fam?lia e a rela??o ecol?gica entre Calliphoridae e Phoridae parasit?ides dentro da ?rea de Prote??o Ambiental de Guapi-Mirim (APA de Guapi-Mirim), em Itabora?. Durante 48 meses, foram realizadas 96 coletas, uma a cada 15 dias. Em todas as capturas, foram utilizadas armadilhas confeccionadas em recipiente pl?stico com 35 cm de altura e 15 cm de di?metro. Cada uma das quatro armadilhas foi suspensa a uma altura de 1,20 m do solo contendo 100g de isca de peixe (sardinha) em decomposi??o por um per?odo de 48 horas. Ap?s cada captura, todos os esp?cimes foram mortos no interior das armadilhas por asfixia utilizando etanol 70%. Em seguida todos os indiv?duos foram acondicionados em potes pl?sticos, contendo etanol a 70% e encaminhados ao Laborat?rio de Transmissores de Leishmanioses (Setor de Entomologia M?dica e Forense), IOC-FIOCRUZ, RJ, em seguida todos os esp?cimes foram separados por dia de coleta, identificadas e quantificadas. Para tal procedimento utilizou-se um microsc?pio estereosc?pico e chaves dicot?micas para a identifica??o da fam?lia e das esp?cies. As an?lises estat?sticas foram feitas utilizando o programa estat?stico Statistica 7.1 (STATSOFT, 2005), atrav?s do teste de Kruskal- Wallis one way ANOVA, teste de qui- quadrado (?2), Mann-Whitney e correla??o de Kendall Tau (p<0,05). Foram utilizados os ?ndices de diversidade de Shannon-Wiener e similaridade de Bray-Curtis, assim como a Dist?ncia Euclidiana. Foram capturadas 4531 moscas pertencentes a dez (10) esp?cies da fam?lia Calliphoridae: Chrysomya megacephala (Fabricius, 1794) (86,40%), Chrysomya albiceps (Wiedemann,1819) (5,72%), Cochliomyia macellaria (Fabricius, 1775) (4,94%), Chrysomya putoria (Wiedemann, 1818) (2,10%), Lucilia eximia (Wiedemann, 1819) (0,30%), Cochliomyia hominivorax (Cocquerel, 1858) (0,22%), Chloroprocta idioidea (Robineau-Desvoidy, 1930) (0,20%), Hemilucilia segmentaria (Fabricius, 1805) (0,04%), Hemilucilia semidiaphana (Rondani, 1850) (0,04%), Lucilia cuprina (Wiedemann,1830) (0,04%). A esp?cie C. megacephala foi aquela que apresentou a maior abund?ncia, a primavera foi a esta??o do ano em que todas as esp?cies mostraram maior prefer?ncia; as temperaturas compreendidas entre 30,5 e 32,40C e a umidade relativa do ar entre 56,8 e 61,7% foram consideradas aquelas onde houve frenesi de oviposi??o; nas luas cheias e novas houve maior ocorr?ncia de moscas capturadas; Megaselia scalaris se utilizou de esp?cies abundantes nas mesmas esta??es do anos em que ela ocorreu com maior frequ?ncia, possibilitando assim maior dispers?o de ovos; a maior disponibilidade de recursos alimentares resultantes da atividade poluidora tempor?ria ofereceram uma melhor condi??o de sobreviv?ncia para as esp?cies os Calliphoridae.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-08T12:42:53Z No. of bitstreams: 1 2011 - Adelmo Carvalho Santana.pdf: 1648328 bytes, checksum: a92ccbc15cc7534a1d274af06e45e8ce (MD5) / Made available in DSpace on 2016-09-08T12:42:53Z (GMT). No. of bitstreams: 1 2011 - Adelmo Carvalho Santana.pdf: 1648328 bytes, checksum: a92ccbc15cc7534a1d274af06e45e8ce (MD5) Previous issue date: 2011-08-23 / This study aimed to identify the level of participation of schools communities from Caatinguinha, Tapera and Bebedouro on a project about recovery of riparian vegetation in the city of Petrolina, Pernambuco. The project was developed by the city hall of Petrolina-PE in partnership with other institutions, among them, the local schools. It?s because it was understood that the school should strengthen its role in transforming the conduct of cooperative activities with the community and even without the existence of a mandatory subject of environmental education into school curricula. Were interviewed students, parents and teachers who have developed some proposed activities in the Project. Through research it was possible to identify the ecological and social consequences resulting from this initiative, could be mentioned, especially, the increasing awareness of environmental issues involved. Was noted the importance of joint actions to bring citizen participation in social processes that are inevitably involved. The performance of schools through the teachers and students was essential to raise awareness of parents and other community members for realization of the actions. It was very clear that the interest and excitement of the students to participate in any phase of the project resulted in more immediate than the greater integration in the classroom, the urge to realize other environmental actions to improve the Community. The study showed that it is not necessary to be an expert to develop environmental education programs in the area. From this experience, other activities have been proposed and developed by schools, considering environmental issues as a political act that prepares citizens to demand social justice, citizenship and respect in relations with nature / Este estudo teve como finalidade identificar o n?vel de participa??o das comunidades escolares de Caatinguinha, Tapera e Bebedouro no Projeto de Recupera??o da Mata Ciliar no Munic?pio de Petrolina-PE. O Projeto foi desenvolvido pela Prefeitura de Petrolina-PE em parceria com outras institui??es, dentre estas as Escolas locais. Isto porque se entendeu que a escola deve fortalecer sua fun??o transformadora com a realiza??o de atividades cooperativas com a comunidade e mesmo sem a exist?ncia obrigat?ria de uma disciplina de educa??o ambiental no curr?culo escolar. Foram entrevistados alunos, pais e professores que desenvolveram algumas atividades propostas no Projeto. Atrav?s da pesquisa foi poss?vel identificar as consequ?ncias ecol?gicas e sociais decorrentes dessa iniciativa, podendo-se citar, especialmente, o aumento da conscientiza??o dos envolvidos nas quest?es ambientais. Observou-se a import?ncia de a??es conjuntas para provocar a participa??o do cidad?o nos processos sociais em que est? inevitavelmente envolvido. A atua??o das escolas atrav?s dos professores e alunos foi imprescind?vel para a sensibiliza??o dos pais e outros membros das comunidades para a realiza??o das a??es. Ficou muito claro que o interesse e euforia dos alunos em participar de alguma fase do Projeto teve como resultado mais imediato al?m do maior entrosamento em sala de aula, o anseio de realizar outras a??es ambientais para a melhoria da Comunidade. O trabalho mostrou que n?o ? necess?rio ser um especialista em educa??o ambiental para desenvolver a??es na ?rea. A partir dessa experi?ncia outras atividades foram propostas e desenvolvidas pelas Escolas, considerando as quest?es ambientais como um ato pol?tico que prepara o cidad?o para exigir justi?a social, cidadania e respeito nas rela??es com a natureza

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-09T11:45:13Z No. of bitstreams: 1 2011 - Vanessa de Almeida Raia.pdf: 1415435 bytes, checksum: 6553cdee2bd5a5f2a363cb3cd11f812d (MD5) / Made available in DSpace on 2016-09-09T11:45:13Z (GMT). No. of bitstreams: 1 2011 - Vanessa de Almeida Raia.pdf: 1415435 bytes, checksum: 6553cdee2bd5a5f2a363cb3cd11f812d (MD5) Previous issue date: 2011-01-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / To fill some gaps about the intrinsic mechanisms of the biology of oviposition of R. (B.) microplus, we evaluated the concentration of lipids and carbohydrates, as well as the variability of lipid in eggs per day of posture. For this, engorged females weighing between 151 and 360 mg were placed in controlled environment (27 ? 1 ? C, 80 ? 5% RH, darkness), and after beginning of laying, three samples of the eggs pool were daily collected, packed and preserved at -20 ? C. Samples 1 and 2, weighing 50 mg each, were used to quantitate lipids and glucose, and to evaluate the lipid profile, respectively; and the third sample, containing 1 g of eggs, was used to quantitate glycogen. The determination of total lipids was performed using phospho-vanillin reagent, while the concentrations of cholesterol, triglycerides and glucose were obtained by colorimetric determination using endpoint enzymatic kit. The level of glycogen was performed using the acid 3,5 dinitrosalicylic reactive . The results were analyzed by nonparametric Kruskal-Wallis and the relationship between the concentrations of substrates and the days of laying was assessed by polynomial regression. There was no significant difference in the concentrations of these substrates in eggs from different days of oviposition, as there was no relationship of these concentrations with the days of laying. After lipid extraction, the samples were performed in thin layer chromatography, high-performance (HPTLC) for neutral lipids and phospholipids. Then, densitometry was performed using the Image Master Total Lab and the daily percentage of each lipid in the samples was calculated. Among the neutral lipids were identified the hydrocarbons, esterified cholesterol, triglycerides, fatty acids, free cholesterol and diacylglycerol. Of these, the cholesterol ester showed the highest variation, it was not detected in eggs at the fifth day of oviposition. Among the phospholipids were detected the phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin fosfatidilinusitol. This latter was absent in eggs of the seventh day onwards. Thus, the different rates of hatchability of eggs from different laying days cannot be attributed to the concentrations of total lipids, although some specific lipids such as cholesterol ester and sphingomyelin might influence the difference in larval hatchability, more researches are needed for clarify the role of these substrates in embryogenesis / Objetivando preencher algumas lacunas sobre os mecanismos intr?nsecos da biologia da oviposi??o de R. (B.) microplus, foram avaliadas as concentra??es de lip?dios e carboidratos, assim como a variabilidade lip?dica dos ovos postos em diferentes dias de postura. Para isso, f?meas ingurgitadas pesando entre 151 e 360 mg foram colocadas em estufa biol?gica sob condi??es controladas (27 ? 1oC, 80 ? 5% UR, escotofase), e ap?s in?cio da postura, tr?s amostras di?rias do ?pool? de ovos foram coletadas, acondicionadas e preservadas a ?20?C. As amostras 1 e 2, pesando 50 mg cada, foram utilizadas para dosar lip?dios e glicose, e avaliar o perfil de lip?dios, respectivamente; a amostra 3, contendo 1 g de ovos foi utilizada para dosar glicog?nio. A dosagem de lip?dios totais foi realizada atrav?s do reagente de fosfo-vanilina, enquanto as concentra??es de colesterol, triglicer?dios e glicose foram obtidas atrav?s de determina??o colorim?trica com a utiliza??o de kit enzim?tico de ponto final. A dosagem de glicog?nio foi realizada atrav?s do reativo ?cido 3,5 dinitrosalic?lico. Os resultados das dosagens foram analisados pelo teste n?o param?trico de Kruskal-Wallis e a rela??o entre as concentra??es dos substratos e os dias de postura foi avaliada atrav?s de Regress?o polinomial. N?o foram constatadas diferen?as significativas entre as concentra??es destes substratos nos ovos de diferentes dias de oviposi??o, assim como n?o houve rela??o dessas concentra??es com os dias de postura. Ap?s extra??o dos lip?dios das amostras, foram realizadas cromatografias em camada delgada de alto desempenho (HPTLC) para lip?dios neutros e fosfolip?dios. Em seguida, a densitometria foi realizada atrav?s do programa Image Master Total Lab e o percentual di?rio de cada lip?dio nas amostras foi calculado. Dentre os lip?dios neutros foram identificados hidrocarbonetos, colesterol esterificado, triglicer?dios, ?cidos graxos, colesterol livre e diacilglicerol. Destes, o colesterol esterificado foi o que apresentou maior varia??o, n?o sendo detectado nos ovos do quinto dia de oviposi??o. Dentre os fosfolip?dios foram detectados o ?cido fosfat?dico, fosfatidiletanolamina, fosfatidilcolina, fosfatidilinusitol e esfingomielina. Sendo este ?ltimo ausente nos ovos do s?timo dia de postura em diante. Assim, as diferentes taxas de eclodibilidade por dia de postura n?o podem ser atribu?das ?s concentra??es de lip?dios totais, embora alguns lip?dios espec?ficos como o colesterol esterificado e a esfingomielina possam ter influ?ncia na diferen?a de eclodibilidade larval, sendo necess?rios mais estudos para eclarecer o papel desses substratos na embriog?nese

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-12T16:49:34Z No. of bitstreams: 1 2011- Manoel Batista Grifo Cabral.pdf: 2116057 bytes, checksum: 0cb1f154d8dd97fcc16aff0de8262b09 (MD5) / Made available in DSpace on 2016-09-12T16:49:34Z (GMT). No. of bitstreams: 1 2011- Manoel Batista Grifo Cabral.pdf: 2116057 bytes, checksum: 0cb1f154d8dd97fcc16aff0de8262b09 (MD5) Previous issue date: 2011-05-19 / This research was done in the Campus of Alegre, Federal Institute of Education, Science and Technology of the State of Espirito Santo. We evaluated the learning by the use of Project Pedagogy. The theme used was the production of lettuce on alternative substrates, commonly used in horticulture regional, developed with students the first year of the course Farming Technician integrated into the school. The development took place in a theoretical and practical involvement with interdisciplinary teaching and administrative servers, covering the bases transverse to the problem. All production steps were planned, implemented and evaluated with the students. The learning evaluation was performed with the use of structured questionnaires distributed temporally. Were characterized in the teaching-learning gains of students observed the results of the evaluations, demonstrating the feasibility of pedagogy in the use of project learning environment. The substrate of improved performance was prepared by the students, and even better that the commercial use in the region. / Esta pesquisa foi realizada no Campus de Alegre, do Instituto Federal de Educa??o, Ci?ncia e Tecnologia do Estado do Esp?rito Santo. Avaliou-se a aprendizagem pela utiliza??o da Pedagogia de Projetos. A tem?tica empregada foi a produ??o de mudas de alface em substratos alternativos, comumente empregados na olericultura regional, desenvolvida com alunos do primeiro ano do Curso T?cnico em Agropecu?ria integrado ao ensino m?dio. O desenvolvimento se deu de forma te?rico-pr?tico com a participa??o interdisciplinar de servidores docentes e administrativos, cobrindo as bases transversais ? problem?tica. Todas as etapas da produ??o foram planejadas, executadas e avaliadas com os discentes. A avalia??o da aprendizagem foi realizada com o emprego de question?rios estruturados temporalmente distribu?dos. Ficaram caracterizados no processo de ensino-aprendizagem ganhos significativos dos discentes, observados nos resultados das avalia??es realizadas, evidenciando a viabilidade na utiliza??o da pedagogia de projeto no ambiente de estudo. O substrato de melhor desempenho foi o elaborado pelos alunos, sendo melhor inclusive que o comercial utilizado na regi?o