Bakgrunden till denna studie är proposition 2020/21:144 som överlämnades till Sveriges riksdag den 18 mars 2021, där regeringen initierar en ny lagstiftning som förväntas träda i kraft den 1 januari 2022. Lagen medför en skyldighet för byggherrar att upprätta och registrera en klimatdeklaration för varje ny bygglovspliktig byggnad som uppförs, där mängden växthusgasutsläpp från byggskedet måste beräknas och redovisas för att byggnaden slutligen ska kunna tas i bruk. Studien har en fastighetsjuridisk utgångspunkt men behandlas ur ett företags- och samhällsekonomiskt perspektiv med syfte att undersöka lagens syfte, tillämpning och konsekvenser ur ett marknadsmässigt perspektiv, samt för att analysera korrelationen mellan lagkravets ikraftträdande och konsekvenser för enskilda branschaktörer av olika storlek. Studien utförs genom en kombination av tre vetenskapliga metodologier som tillsammans utgör ett empiriskt underlag för vidare analys. Studiens resultat visar på att lagens ikraftträdande kommer att medföra konsekvenser för samtliga aktörer inom bygg- och fastighetsbranschen, och enskilda branschföretag kommer bli tvungna att implementera kostnadsdrivande anpassningsåtgärder för att uppfylla det nya lagkravet. Detta riskerar särskilt att orsaka problem för de aktörer som inte redan arbetar aktivt med klimatfrågan, och riskerar även särskilt att drabba mindre företag. Detta kan i sin tur orsaka ogynnsamma konkurrensförhållanden på en marknad influerad av oligopolliknande tendenser, vilket även kan medföra samhällsekonomiska effekter, exempelvis i form av stagnation på bostadsmarknaden. I kontrast till detta framhäver dock klimatkalkyler som integrerar ekonomiska och ekologiska nyttoeffekter att införandet av lagen om klimatdeklaration för byggnader på längre sikt kan anses utgöra en lönsam investering ur både ett företags- och samhällsekonomiskt perspektiv. / The background of this study is proposition 2020/21:144 which was submitted to the Swedish Parliament on March 18th 2021, where the government initiates a new law that is expected to enter into force on 1st of January 2022. The law entails an obligation for property developers to prepare and register a climate declaration for each new building subject to a building permit, where the carbon footprint from the construction must be calculated and reported in order to take the building into use. The study has a legal outset towards applicable law but is mainly treated from a business and socio-economic perspective in order to examine the law’s purpose, application and consequences from a corporate view, and to analyze the correlation between the legal requirements and consequences based on company size. The study is carried out through a combination of three scientific methodologies which together form an empirical basis for further analysis. The essence of the study shows that the new legal requirements will affect all different organisations within the construction and real estate industry, and will demand cost-driven adaptation measures to meet the new legal requirements. This is particularly likely to cause problems for those companies who have not already implemented an eco-friendly strategy for sustainability and climate awareness, and will also most likely particularly affect smaller companies in a negative way. This in turn can cause unfavorable conditions of competition in a market influenced by oligopol tendencies, with socio-economic effects, such as stagnation in the housing market, as a result. In contrast, climate calculations that integrate economic and ecological benefits emphasize that the introduction of the Climate Declaration Act for buildings in the longer term could be seen as a profitable investment from both a business and socio-economic perspective.

Durch die Lebenszyklusanalyse von Bauwerkintegrierten Photovoltaik(BIPV)-Fassaden werden deren ökologische Vorteile gegenüber konventionellen Fassadenlösungen aufgezeigt. Die frühzeitige Analyse zu Beginn des Planungsprozesses ermöglicht es, die individuellen Potentiale von Baukörpern zu ermitteln und ermöglicht sichere Planungsentscheidungen durch Kennwerten. In dem Beitrag erfolgen Lebenszyklusanalysen an einem Fallbeispiel mit unterschiedlichen Betrachtungsebenen und methodischen Ansätzen. Es wird geprüft, welche Ansätze die Entscheidungsfindung von Architekt:innen und Planer:innen im Entwurfsprozess am besten unterstützen. Außerdem werden anwendungsbezogenen Erkenntnisse und Randbedingungen bei der Bilanzierung der Treibhausgasemissionen über den gesamten Lebenszyklus von BIPV-Fassaden identifiziert. / Life cycle analysis of building-integrated photovoltaic (BIPV) façades demonstrates their ecological advantages over conventional façade solutions. The early analysis at the beginning of the planning process makes it possible to determine the individual potential of building structures and enables reliable planning decisions based on characteristic values. In this article, life cycle analyses are carried out using a case study with different perspectives and methodological approaches. It is examined which approaches best support the decision-making of architects and planners in the design process. In addition, practical findings and boundary conditions for balancing greenhouse gas emissions over the entire life cycle of BIPV façades are identified.

A comparison analysis conducted via COMPASS life cycle analysis software has indicated that a one-way crate design, rather than a reusable crate design, is in fact the more environmentally friendly packaging system. These results can be interpreted differently, however, as the manufacturer of said crate designs must confirm what impact indicators they would like to reference as environmental goalposts. The conducted analysis provides insight into what the environmental impacts of each packaging system look like as packaging at all three system levels has been identified as a means of reducing environmental impacts globally. As such, the manufacturer of said crate designs would like to establish a baseline understanding of what the environmental impact of their products look like. Version 3.5 of COMPASS LCA software was utilized, which references ecoinvent 3.9.1 LCA databases as well as U.S. Life Cycle Inventory (USLCI) LCA methodologies. These findings are significant as they affirm that all packaging system inputs and product life-cycle phases must be carefully considered as they impact every impact indicator score differently.

Coiled coils are a well-known protein structure prevalent in eukaryotic function, synthetic applications, and de novo protein design. Coiled-coil folding is often described using heptad repeat positions labeled abcdefg where a and d positions occupy the interface between the coils, e and g positions flank the interface, and the b, c, and f positions face the solvent-exposed surface. The a, d, e, and g positions have been extensively studied in the coiled-coil literature. There is a lack of investigation on the impact of the b, c, and f positions on coiled-coil folding. Chapter 1 is an introduction to the heptad repeat of coiled coils and the impact on folding of each heptad repeat position. In Chapter 2 we introduce a non-covalent interaction among the b, c, and f positions of a coiled-coil trimer that significantly enhances thermodynamic stability. We identify characteristics of the f-position residue (hydrogen bond donating ability and hydrophobicity) that lead to the greatest amount of stability. Chapter 3 introduces crystal structures and molecular dynamic simulations of the interaction to identify the mechanism of stabilization. Further thermodynamic studies find a key salt-bridge interaction between the b and c positions that are influenced by the f-position residue. Chapter 4 explores the impact of salt on the non-covalent interaction and determines that the interaction is sensitive to salt screening and is ionic in nature. It also explores more characteristics of the f-position amino acid, in particular the hydrogen bond donating component. In Chapter 5 we insert the solvent-exposed interaction into helix bundles of differing length and oligomeric state. We find that stability is not only dependent upon amino acid identity but also the length and stoichiometry of a coiled coil.

The paper presents the development methodology of a hydrostatic pump/motor for use in Parker Hannifin’s advanced series hydraulic hybrid transmissions for medium and heavy duty commercial vehicles. With Parker’s established bent-axis pump/motor technology for heavy duty mobile applications as a basis, it describes the main stages of further development and qualification for demanding automotive main drive transmissions. Parker’s APQP based, customer focused product development model was employed for this development which resulted in the variable bent-axis pump/motor C24 for open circuit hydrostatic transmissions. Positive customer results from a large fleet of in-service refuse collection trucks and parcel delivery vans with Parker advanced hydraulic hybrid drive systems using C24 pumps/motors serve as evidence of Parker’s product development model effectiveness. High reliability, good fuel economy, increased productivity and long brake life of the vehicles can directly be traced back to the streamlined, front-loaded and iterative development model.

Marine renewable energy promises to provide a significant contribution to the future electricity supply. It is estimated that 17% of today's UK electricity demand could be generated from wave and tidal sources. The ambition to harvest this resource is in the public interest, as it eases the pressures on energy security, holds the potential to reduce carbon emissions and has the prospect to create a new UK industry sector worth £15 billion. From an engineering perspective, marine energy is one of the least developed renewable energy technologies and has to be regarded as unproven. The reliability of components and devices in the harsh marine environment is one of the main engineering challenges. Reliability assessments and the assurance of acceptable reliability levels are dependant on the adequacy of failure information, which is scantily available for marine energy. This thesis shows that large failure rate uncertainties impede the reliability assessment for wave energy converters and how a suite of experimental, numerical and statistical methods can be applied to improve scarcely available reliability information. The analysis of component load conditions identifies fatigue as failure mode of concern and the fatigue life of mooring lines and marine power cables is quantified in a floating wave energy application. A Bayesian statistical approach and dedicated service-simulation component testing is proposed, and implemented to improve the quality of reliability estimates and to provide relevant data and assurance. The methods presented, along with the results, will assist reliability assessment and design during early development stages, and will inform the prediction of maintenance requirements during operation. Reliable marine energy systems will be the technical enabler for the successful transition of prototype devices to a commercially viable marine energy industry.

Les canaux calciques dépendants du voltage CaV font partie de la famille structurale des canaux ioniques à 6 segments transmembranaires. Tout comme les canaux potassiques Kv, les canaux CaV possèdent une série de résidus chargés dans l’hélice S4 de chaque domaine ou sous-unité qui conférerait à la protéine une sensibilité aux changements de voltage. De plus les hélices S6 tapissent la paroi du pore et forment la porte d’activation de la protéine. Comment le mouvement des hélices S4 se traduit par l’ouverture de la porte d’activation des hélices S6 demeure une question encore non résolue. Suite à la publication de la structure cristalline du canal Kv1.2 en 2005, le groupe de MacKinnon a proposé que le mouvement des hélices S4 est mécaniquement couplé à la porte d’activation S6 à travers le glissement de l’hélice amphiphile S4-S5 selon un mécanisme nommé couplage électromécanique (Long et al. 2005b). Dans le but de déterminer si la région S4-S5 joue un rôle dans l’activation du canal calcique CaV2.3, nous avons étudié, par la méthode d’analyse cyclique de mutations doubles (« Double Mutant Cycle Analysis », (Horovitz 1996)), le couplage entre la boucle S4-S5 et l’hélice S6 du domaine II de ce canal. Les mesures d’énergies d’activation, ΔGact, obtenues en présence des sous-unités auxiliaires CaVα2δ et CaVβ3 ont affiché un couplage significatif pour l’activation entre les paires de résidus V593G/L699G, V593G/A700G, V593G/A702G, S595G/V703G L596G/L699G, L596G/A700G, L596G/I701G, L596G/A702G, L596G/V703G, L596G/D704G, M597G/I701G, et S602G/I701G. Aucune de ces paires de résidus n’a affiché de couplage lors de l’inactivation, suggérant que les effets observés sont spécifiques au mécanisme d’activation. Mis ensemble, ces résultats suggèrent que la boucle IIS4-S5 et l’hélice IIS6 interagissent et jouent un rôle déterminant dans l’activation de CaV2.3. / Voltage dependent calcium channels share a strong structural homology with voltage gated potassium channels. Both families present a conserved series of charged residues present in the S4 helix of each domain that most certainly accounts for the voltage sensitivity of these proteins. Moreover, in both cases, the S6 helices seem to be lining up the pore. How does the movement of the S4 sensors translate into channel opening remains elusive in Ca2+ channels. Following the publication of the crystal structure of the Kv1.2 channel in 2005, the group of Roderick MacKinnon proposed that the voltage sensor is mechanically coupled to the S6 pore through the amphipathic S4-S5 helix that crosses over the S6 inner helix from the same subunit. To determine if the S4-S5 linker, that runs parallel to the membrane plane inside the cell in the Kv1.2 three-D structure, plays a role in the activation of the CaV2.3 calcium channel, we have studied by double mutant cycle analysis the coupling between the S4-S5 linker and the S6 helix of domain II of this channel. The activation energies, Gact, obtained from classical two electrode voltage clamp experiments in the presence of auxiliary subunits CaV2 and CaV3 displayed significant activation coupling coefficients for the pairs of residues V593G/L699G, V593G/A700G, V593G/A702G, S595G/V703G L596G/L699G, L596G/A700G, L596G/I701G, L596G/A702G, L596G/V703G, L596G/D704G, M597G/I701G, and S602G/I701G. None of these pairs displayed significant coupling in the inactivation mechanism, suggesting that the effects observed were specific to activation. Altogether, our results strongly suggest that the S4-S5 linker and the S6 helix of domain II are actively involved in the activation of CaV2.3.

Ett hållbart företagande berör tre olika dimensioner: sociala dimensionerna, ekologiska dimensionerna och de ekonomiska dimensionerna.  För att arbeta mot ett hållbart företagande behöver ett företag kunna balansera dessa tre dimensioner samtidigt. Detta examensarbete syftar till att utifrån ett hållbart företagande undersöka de sociala, ekonomiska och ekologiska dimensionerna för att fastställa Bra miljötekniks befintliga hållbarhetsarbete. Studien analyserar hur de tre hållbarhetsdimensionerna integrerar med varandra. Detta för att få en ökad förståelse kring hur företaget bättre ska kunna anpassa sin verksamhet mot ett hållbart företagande.   För att skapa en djupare förståelse kring ämnet genomfördes en litteraturstudie. Litteraturinsamlingen består av avhandlingar och akademisk litteratur. Studien innehåller kvalitativa intervjuer med en semistrukturerad utformning. Syftet med intervjuerna var att skapa ökad förståelse kring hur företaget arbetar med sina sociala åtaganden. Både en direkt-intervju och en telefonintervju genomfördes.   Sociala aspekter och förhållanden vid företaget undersöks i studien genom en semistrukturerad intervju. De ekonomiska aspekterna undersöks genom viktning av tung lastbil, drivmedel, utsläpp och kostnader. Den ekologiska dimensionen undersöks med hjälp av verktyget livscykelanalys (LCA). Detta verktyg används för att öka förståelsen kring hur företagets aktiviteter påverkar i form av kostnader och miljöavtryck.   I den ekonomiska dimensionen kunde det tydligt utläsas att ingen av investeringarna var relativt stor i jämförelse med företagets resultat. Byte av bränsle till hydrerad vegetabilisk olja är det bästa alternativet taget ur ett perspektiv för hållbart företagande. De sociala dimensionerna undersöktes i form av aktiva val, etik, moral och inställning. Det framgick att stort ansvar har lagts på moderföretaget i Norge där all produktion sker. Inställningen var att; det sköter dem. Slutsatsen blev att företaget saknade kunskap kring vad de sociala dimensionerna innefattar, vilket är inte ovanligt enligt tidigare forskning. De sociala delarna består av mjuka delar och kräver därför en mer demokratisk och öppen process för att utvecklas framgångsrikt.     Att aktivt arbeta med hållbart företagande är resurskrävande för hela organisationen och dess processer. Ständig uppdatering och kontroll är ett måste. Då lönsamhet kan vara svårt att utläsa i ett kortsiktigt perspektiv finns en förståelse för att företag väljer att lägga resurser på annat.   Fördelar när det kommer till arbete med hållbart företagande är att det i det långa loppet genererar både ekonomisk lönsamhet och kompetensutveckling. Dock bör tilläggas att det främsta incitamentet till ett hållbart företagande är att kunna ge framtida generationer förutsättningarna att leva av och på vår planet precis som vi själva gjort. / Sustainable entrepreneurship involves three different dimensions in sustainable development: social dimensions, ecological dimensions and economic dimensions. In order to work towards sustainable entrepreneurship the firm needs to balance these three areas of responsibility simultaneously. This study examines the social, economic and ecological dimensions of sustainable enterprise, based on sustainable entrepreneurship. The study analyses how the three sustainability dimensions integrates with each other in order to gain a better understanding of how to better adapt their business to sustainable entrepreneurship.   In order to obtain a comprehensive base a literature study was first conducted. The literature reviews consists of dissertations and literature studies. The study is also based on qualitative interviews with a semi-structured design. The purpose of the interviews was to get a better understanding of the present social commitment within the firm. Both a direct interview and a telephone interview were conducted.   Social aspects and conditions at the company were examined in the study through a semi-structured interview. In the economic aspects, heavy truck, fuel, emissions and costs for sustainable entrepreneurship were weighted. The ecological dimension is investigated through using the Lifecycle Assessment (LCA) as a tool. When using a LCA a company can get a greater understanding of how their activities affect the environment and where the effect has the most impact.    In the economic dimension, it was clear that none of the investments were relatively large in comparison to the company's earnings. Changing the fuel to HVO is clearly the best option taken from a perspective for sustainable entrepreneurship. The social dimensions were examined in terms of active choices, ethics, morals and attitude. It was very clear that great responsibility lies with the mother-company in Norway where all production takes place. The company lacked knowledge in how to affect the social dimensions and what they included. The social dimensions consist of many soft parts, a more democratic and open process is needed to create a successful result.   Working actively with sustainable entrepreneurship is demanding resources for the entire organization and its processes, as well as continuous updating and control. Since profitability in numbers can be difficult to read in a short-term perspective many companies choose to put resources on other things.    Advantages for organizations that work with sustainable entrepreneurship are that it generates both economic profitability and competence development in a long-term perspective. But most importantly, future generations must be given the conditions to live on and off our planet just as we did.

Este trabalho apresenta a avaliação das externalidades relativas ao ciclo produtivo da cana de açúcar, dando um enfoque para a questão da geração de energia elétrica. Os objetivos principais do estudo são realizar um levantamento uma análise das externalidades sociais, ambientais e econômicas do ciclo produtivo da cana-de-açúcar desde sua fase inicial (período agrícola) até a conversão energética da biomassa em energia elétrica (objeto de ênfase nesse projeto). Repartindo os impactos, quando possível nos subsistemas elaborados a partir da análise do ciclo produtivo do setor, cujos resultados de uma forma geral eram até então conhecidos, mas nunca segregados e tratados de forma integrada sob a ótica de avaliação das externalidades relacionadas à atividade de produção. A geração de eletricidade excedente traz consigo externalidades positivas e negativas. Elas envolvem aspectos prejudiciais sob a ótica das emissões atmosféricas sobre a saúde humana e o meio biótico; positivo, mediante a utilização de fontes renováveis para geração de eletricidade provendo o deslocamento de derivados do petróleo tanto para geração de energia térmica quanto de elétrica, reduzindo assim o consumo de combustíveis fósseis que são os principais componentes fomentadores da acentuação do efeito estufa; além dos benefícios associados à geração distribuída, discutidos neste trabalho. Ambas qualificam esta forma de geração dentro dos requisitos do mecanismo de desenvolvimento limpo, que será avaliado com a visão de ser um instrumento para internalizar o benefício de gerar energia com recursos renováveis. Dos impactos avaliados correspondentes ao ciclo produtivo da cana-de-açúcar, 32,68 % estão associados com a etapa de geração de eletricidade. Os principais impactos e efeitos avaliados na etapa qualitativa e quantitativa deste trabalho nas externalidades de produção foram ratificadas como efeitos predominantes também nas externalidades avaliadas via simulação computacional (ECOSENSE LE) do projeto ExternE. / This work presents the evaluation of externalidades in production from sugarcane life cycle, with emphasis at electric power generation. The main objective of this study is to assess the health, social, environmental and economical externalities related with the production process of sugarcane industry from the start point of the productive chain (agricultural period) until the energy conversion of the biomass in electric power (main emphasis). Distributing the impacts, when possible, in subsystems that main productive cycle were divided, whose results, in general, were known but never segregated and treated by an integrated view under the optics of externalities evaluation at the production activity chain at sugarcane sector. The surplus generation of electricity brings with itself positive and negative externalities. The negative ones involve harmful aspects under the atmospheric emissions and human health and the biotic environment; as positive ones, are the use of renewable sources for electricity generation providing displacement of fossil fuels and indirectly thermal and electrical energy, reducing the consumption of this kind of fuels that are the main promoting components from the accentuation of the greenhouse effect and the benefits associated to the distributed generation, also discussed in this work. Both of these positive aspects, qualify this form of generation to participate at the clean development mechanism, that it will be treated as an instrument for incorporate the benefit of generating energy with renewable resources. From the main productive chain impacts related to the life cycle of the sugarcane, 32,68 % are associated with the stage of electricity generation. The main impacts assessed in the qualitative and quantitative way as production externalities were confirm as predominant effects also using the computational simulation tool (ECOSENSE LE) from the ExternE project.

Submitted by Maicon Juliano Schmidt (maicons) on 2015-04-18T14:41:17Z No. of bitstreams: 1 Josiane Reschke Pires.pdf: 4785187 bytes, checksum: 5dcede27f6c8d94cee536d3dfbfa047d (MD5) / Made available in DSpace on 2015-04-18T14:41:17Z (GMT). No. of bitstreams: 1 Josiane Reschke Pires.pdf: 4785187 bytes, checksum: 5dcede27f6c8d94cee536d3dfbfa047d (MD5) Previous issue date: 2013-03-21 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / PROSUP - Programa de Suporte à Pós-Gradução de Instituições de Ensino Particulares / A preocupação com a sustentabilidade sugere a busca pela otimização dos recursos naturais. Um dos aspectos necessários é adequar as edificações para o clima em que estão localizadas, reduzindo o consumo energético da habitação e ampliando o conforto ambiental. O objetivo desta pesquisa é investigar a viabilidade técnica e econômica da aplicação de critérios de desempenho térmico em edificações residenciais. A investigação foi centrada na identificação dos custos e da energia no ciclo de vida de projetos simulados com soluções baseadas em conforto ambiental passivo (natural) e ativo (artificial), verificando a influência no consumo de energia da edificação. Foi aplicada em projetos de edificações residenciais verticais e horizontais, de classe média e baixa, em dois climas distintos (Porto Alegre e Bento Gonçalves, RS), examinando o tipo de envelope, as absortâncias e o uso de elementos construtivos. Para análise do desempenho térmico foi adotada a norma de desempenho NBR 15575 e método graus-hora, com modelagem BIM, utilizando o Revit/Autodesk e para as simulações térmicas foi empregado o EnergyPlus, software especializado em análise energética para edificações. O ciclo de vida dos projetos propostos foi analisado considerando-se um período de 50 anos, comparando os custos e a energia incorporada das alternativas. Como um segundo parâmetro de sustentabilidade, também se analisou a eficiência energética conforme o selo de eficiência energética Procel Edifica. Os resultados indicam que com o aumento do isolamento da envoltória se verifica uma melhoria do conforto térmico, de até 54%, e economia de energia, em ambos os climas, com um período de retorno de 2 a 6 anos, em alguns casos. / The concern with sustainability brought the need for optimization of the energy resources to reduce the consumption of electric energy and of natural resources. One of the aspects is the need to adapt the buildings to the climate in which they are located, reducing the energy consumption of housing. The aim of this research is to investigate the technical and economic viability of applying some criteria of thermal performance in residential buildings. The methodology is based on the identification of the costs and energy in the life cycle of projects based on environmental comfort on passive (natural) and active (artificial) ways, detailing the influence on energy consumption of the building. It was applied in residential projects, both vertical and horizontal, of lower and middle class, in two distinct microclimates (Porto Alegre and Bento Gonçalves, southern Brazilian cities), examining alternatives of envelope, insulation and the use of architectural façade elements. To analyze the thermal performance was adopted the Brazilian standard NBR 15575 and degree-hour method, with BIM modeling through Revit/Autodesk and for the thermal simulation Ecotect/Autodesk and EnergyPlus. The life cycle of the proposed case studies was analyzed by considering a period of 50 years, comparing the costs and embodied energy of alternatives. As a second measure of sustainability, energy efficiency was also analyzed with the Brazilian standard of energy efficiency Procel Edifica. The results showed that with the increment of envelope’s insulation there are improvement of thermal comfort, of 54%, and energetic economy, in both climates, with a payback period of 2 to 6 years, in some cases.