Submitted by MARISE PEREIRA DA SILVA (msteix@fmb.unesp.br) on 2016-09-27T14:30:08Z No. of bitstreams: 1 Defesa Doutorado Marise Pereira da Silva - 2016.pdf: 2312700 bytes, checksum: 2943adfd4da085f54c9f7ba686299e2d (MD5) / Approved for entry into archive by Ana Paula Grisoto (grisotoana@reitoria.unesp.br) on 2016-09-28T18:44:37Z (GMT) No. of bitstreams: 1 silva_mp_dr_bot.pdf: 2312700 bytes, checksum: 2943adfd4da085f54c9f7ba686299e2d (MD5) / Made available in DSpace on 2016-09-28T18:44:37Z (GMT). No. of bitstreams: 1 silva_mp_dr_bot.pdf: 2312700 bytes, checksum: 2943adfd4da085f54c9f7ba686299e2d (MD5) Previous issue date: 2016-06-24 / Item merged in doublecheck by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2017-03-22T17:41:47Z Item was identical to item(s): 150213, 144099 at handle(s): http://hdl.handle.net/11449/149817, http://hdl.handle.net/11449/144224 / A doença hepática gordurosa não alcoólica (DHGNA) é a alteração mais comum do metabolismo hepático, associada à obesidade, resistência à insulina e à síndrome metabólica. Compreende amplo espectro de alterações, que tem em comum a esteatose, em indivíduos que não consomem álcool. Além da sobrecarga de ingestão calórica, outros fatores como alterações do metabolismo do ferro podem estar relacionados à patogênese ou progressão da DHGNA. Analisamos no presente estudo as possíveis relações de marcadores do metabolismo do ferro com a intensidade das lesões hepáticas na DHGNA em indivíduos obesos. Foram estudados 88 indivíduos adultos, de ambos os gêneros, obesos (IMC >30), com diagnóstico histológico de DHGNA, acompanhados no ambulatório de Gastroenterologia e Hepatologia da FMB-Unesp. Os marcadores do metabolismo do ferro avaliados foram: níveis séricos de ferro, ferritina e transferrina, bem como o índice de saturação da transferrina (IST). A presença e intensidade da deposição de ferro no parênquima hepático foram investigadas pelo método histoquímico do Azul da Prússia (Perls). A intensidade da esteatose e o grau de fibrose foram avaliados nas biópsias hepáticas. Dos 88 indivíduos avaliados, 31 (35,2 %) apresentaram aumento dos níveis séricos de ferritina e 12 (13,6%) apresentaram valores de IST acima de 45 %. A avaliação histológica, das biópsias hepáticas, demonstrou a presença de fibrose em 48/88 casos (54,54%), sendo 21/88 (23,86%) de intensidade leve e 27/88 (30,68%) casos moderada/grave. A investigação da presença de hemossiderina demonstrou depósitos granulares finos no citoplasma dos hepatócitos periportais em 17/88 biópsias (19,32%). Não houve associação da presença de hemossiderina no parênquima hepático com a intensidade da fibrose. A análise de regressão logística identificou a presença de DMT2 ou glicemia de jejum > 100, dislipidemia e a presença de inflamação mista (lobular e portal) como fatores independentemente associados com a fibrose. Os resultados do presente estudo demonstraram que a presença do ferro no parênquima hepático é discreta em indivíduos obesos com DHGNA e provavelmente provavelmente não relacionada à patogênese ou progressão das lesões hepáticas que ocorrem nesta entidade. / The nonalcoholic fatty liver disease (NAFLD) is the most common modification of hepatic metabolism associated with obesity, insulin resistance and the metabolic syndrome. It comprises wide spectrum of changes, which have in common steatosis in individuals who do not consume alcohol. Besides calorie intake overload, other factors such as iron metabolism disorders may be related to the pathogenesis or progression of NAFLD. We analyzed in the present study the possible relationship of iron metabolism markers with the severity of liver injury in NAFLD in obese individuals. 88 adult subjects were studied, of both genders, obese (BMI> 30), with histological diagnosis of NAFLD, from ambulatory of gastroenterology and hepatology - FMB-Unesp. The markers of iron metabolism evaluated were: serum iron, transferrin and ferritin as well as the transferrin saturation index (STI). The presence and intensity of iron deposition in the liver parenchyma were investigated by histochemical method of Prussian blue (Perls). The intensity of steatosis and degree of fibrosis in liver biopsies were evaluated. Of the 88 individuals evaluated, 31 (35.2%) showed increased serum ferritin levels and 12 (13.6%) presented IST values above 45%. Histological evaluation of liver biopsies showed the presence of fibrosis in 48/88 cases (54.54%) and 21/88 (23.86%) mild and 27/88 (30.68%) moderate cases /serious. The investigation showed the presence of hemosiderin deposits in fine granular cytoplasm of periportal hepatocytes in 17/88 biopsies (19.32%). There was no association between the presence of hemosiderin in the liver parenchyma with the intensity of fibrosis. Logistic regression analysis identified the presence of T2DM or fasting glucose > 100, dyslipidemia and the presence of mixed inflammation (lobular and portal) as factors independently associated with fibrosis. The results of this study demonstrated that the presence of iron in the liver parenchyma is slight in obese subjects with NAFLD and probably not related to the pathogenesis or progression of liver damage occurring in this entity.

La sécheresse oculaire est une pathologie fréquente qui peut impacter fortement la qualité de vie des patients. D’origine multifactorielle complexe, elle présente de nombreuses étiologies et son diagnostic est difficile en raison de présentations cliniques variées, en termes de symptômes et de tests cliniques, et en l'absence d'un marqueur spécifique. En outre, elle ne bénéficie actuellement que d’une seule molécule thérapeutique, cependant non curative, la ciclosporine. Devenant un réel problème de santé publique, la recherche visant à une meilleure compréhension des mécanismes sous-jacents à son apparition reste indispensable. L'état actuel des connaissances sur la sécheresse oculaire reconnaît à l'inflammation et à l'hyperosmolarité un rôle central. Ce travail de thèse a eu pour objectif d'étudier le comportement pro-inflammatoire des cellules conjonctivales dans un modèle in vitro classique de sécheresse oculaire induite par une hyperosmolarité saline. Nous avons ainsi montré que les cellules conjonctivales sécrètent une chimiokine pro-inflammatoire majeure, CCL2/MCP-1, et que cette induction est totalement sous la dépendance du facteur de transcription osmoprotecteur NFAT5/TonEBP ainsi que partiellement liée à la voie des MAPKs et de NFκB. Nous avons également observé que cette induction était en partie inhibée par la ciclosporine et la dexaméthasone. Nous avons ensuite complété ce travail par l'étude de l'inflammasome à la fois sur ces cellules et sur des monocytes, type cellulaire majeur ciblé par CCL2, en utilisant différents activateurs classiques dans le modèle d’hyperosmolarité. Nos résultats ont montré l’absence d’activation de l’inflammasome par l'hyperosmolarité seule. En revanche, l'hyperosmolarité aurait un rôle inhibiteur de l’inflammasome dans les monocytes. Ces résultats ouvrent des pistes nouvelles d’exploration afin de mieux comprendre les relations entre monocytes et cellules épithéliales de la conjonctive. Ils nous ont permis d'identifier le rôle incontournable de cellules épithéliales conjonctivales dans l'apparition ou le maintien de l'inflammation dans la pathologie et de caractériser certaines voies intracellulaires impliquées dans ce processus, ouvrant de nouvelles perspectives en termes de thérapeutiques. / Dry eye disease is a common condition that significantly impacts the quality of life of patients. It is a complex multifactorial disease with many etiologies and a diagnosis recognized as difficult due to the multiple clinical presentations in terms of symptoms and clinical tests, and lacking of a reliable and specific marker. There is currently only one therapeutic molecule to treat this disease, cyclosporin. However it is not curative. Dry eye disease is becoming an important public health problem and it is necessary to focus research on its underlying mechanisms, as they remain largely unknown. The current state of knowledge in dry eye disease recognizes inflammation and hyperosmolarity as central elements in the pathology. This work aimed to study the pro-inflammatory behavior of conjunctival cells in an in vitro model of dry eye disease by NaCl-induced hyperosmolarity. We showed that conjunctival cells secrete a major pro-inflammatory chemokine known as CCL2/MCP-1. This induction depends entirely on the osmoprotectant transcription factor NFAT5/TonEBP and is partially related to the MAPKs and the NFκB pathways. We showed that the process could be partially inhibited by cyclosporin and dexamethasone. We also studied the inflammasome in conjunctival cells and monocytes, the major cell type targeted by CCL2, using various classical inflammasome activators in this hyperosmolarity model. We did not observe any inflammasome activation induced by hyperosmolarity alone. On the other hand, hyperosmolarity shows signs of inhibiting inflammasome activation in monocytes. These results open new ways of understanding the relations between monocytes and conjunctival epithelial cells. They highlight the crucial role of the conjunctival epithelial cell type in the inflammation process in dry eye disease and make it possible to characterize intracellular pathways involved in this process, opening new therapeutic prospects.

No description available.

Tyrimo klausimas: Kokie yra bendravimo sunkumai tarp slaugytojų ir onkologinėmis ligomis sergančių vaikų ir jų tėvų ? Tyrimo tikslas: nustatyti slaugytojų ir onkologinėmis ligomis sergančių vaikų bei jų tėvų bendravimo poreikį ir pasitenkinimą bendravimo kokybe. Darbo uždaviniai: 1. Įvertinti vaikų, sergančių onkologinėmis ligomis, bendravimo poreikį ir pasitenkinimą bendravimo kokybe gydantis ligoninėje. 2. Įvertinti tėvų, kurių vaikai serga onkologinėmis ligomis, bendravimo poreikį ir pasitenkinimą bendravimo kokybe būnant ligoninėje. 3. Įvertinti slaugytojų pasitenkinimą bendravimo kokybe su onkologinėmis ligomis sergančiais vaikais ir jų tėvais. 4. Nustatyti veiksnius, įtakojančius bendravimą tarp slaugytojų ir onkologinėmis ligomis sergančių vaikų bei jų tėvų. Darbo metodika: Tyrimas buvo atliekamas Kauno medicinos universiteto klinikos, Vaikų II vidaus ligų skyriuje, onkohematologiniame poskyryje, 2010 m. sausio - kovo mėn. gavus skyriaus vedėjos, gydymo įstaigos sutikimus bei KMU Bioetikos centro leidimą. Tyrime dalyvavo 9 respondentai: 3 mamos bei 3 vaikai, sergantys onkologine liga ir 3 bendrosios praktikos slaugytojos, dirbančios su vaikais, sergančiais onkologinėmis ligomis (KMUK Vaikų II vidaus ligų skyriuje, onkohematologiniame poskyryje). Tyrimui atlikti buvo naudojama kokybinis fenomenologinis tyrimo metodas. Tyrimo metodų trianguliacija: giluminis pusiau struktūrizuotas interviu (atskirai sudaryti klausimai slaugytojams, vaikams bei tėvams, dokumentų... [toliau žr. visą tekstą] / Question of the research: What are the difficulties of the communication between the nurses and oncologic desease having children and their parents? Aim of the research: to find out the nurses„, oncologic desease having children and their parents communication needs and satisfaction of the communication quality. Tasks: 5. Find out the communication needs and the satisfaction of the communication quality of the oncologic desease having children during the treatment in the hospital. 6. Find out the parents„ whose children have oncologic desease communication needs and the satisfaction of the communication quality in the hospital. 7. Find out the nurses„ satisfaction of the communication quality with oncologic desease having children and their parents. 8. Find out the factors that are influencing the communication between the nurses and the oncologic desease having children and their parents. Methods: The research was done in Kaunas University of Medicine Hospital, Department of the Children II Internal Medicine, Onco-hematology subdivision, in January – March of 2010, with the permition of the head of the department and the head of the hospital and KUM Bioethics center. There were 9 respondents: 3 children having the oncological desease, 3 mothers and 3 nurses who are working in the Department of the Children II Internal Medicine, Onco-hematology subdivision. The research was carried out using the triangulation of research methods: deep half-structured 6 interview (separate... [to full text]

Este trabalho é uma contribuição importante para o desenvolvimento de um modelo numérico de arênquima pulmonar capaz de simular manobras de ventilação mecânica. O pulmão é um órgão estruturalmente complexo e hierarquizado. Por isso uma revisão bibliográfica multidisciplinar foi realizada. A revisão apresenta as propriedades mecânicas do parênquima, sua morfologia e os efeitos da tensão superficial para a contração e adesão dos septos interalveolares. Um aspecto importante para o modelo de alvéolo é um modelo de contato com adesão causada pela tensão superficial. Um modelo de contato adesivo simplificado foi desenvolvido e simulado em uma estrutura com parâmetros de mesma ordem de grandeza de um alvéolo real. A simulação foi realizada com o método dos elementos finitos não-linear e foi necessário empregar o método da corda para evitar divergência em pontos limites. Os resultados numéricos se aproximaram de resultados experimentais globai no pulmão com pressões de mesma ordem de grandeza. / This work is an important step on the development of a computational model of lung parenchyma capable to simulate mechanical ventilation maneuveres. The lung has a complex and hierarchized structure. Therefore a multidisciplinary literature review was held. The review presents the mechanical properties of the parenchyma, its morphology and the effects of surface tension to septums contraction and adhesion. An important aspect for the alveolus model is a contact model which includes the adhesion caused by surface tension. Thus a simplified model was developed and then simulated in a structure with properties of the same order of magnitude of a real alveolus. The simulation was performed with the nonlinear finite element method. The implementation of the arc-length method was also necessary in order to prevent diversion at limit points. The numerical results were close to whole lung experimental results with pressure levels of the same order of magnitude.

Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-10-08T21:42:51Z No. of bitstreams: 2 Dissertação - Bruna Lígia Ferreira de Almeida - 2013.pdf: 856377 bytes, checksum: bb3d1938f40e066ea7486b9a556bcf59 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-10-09T11:14:40Z (GMT) No. of bitstreams: 2 Dissertação - Bruna Lígia Ferreira de Almeida - 2013.pdf: 856377 bytes, checksum: bb3d1938f40e066ea7486b9a556bcf59 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-10-09T11:14:40Z (GMT). No. of bitstreams: 2 Dissertação - Bruna Lígia Ferreira de Almeida - 2013.pdf: 856377 bytes, checksum: bb3d1938f40e066ea7486b9a556bcf59 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-04-05 / The feminization of the epidemic have many consequences, among them, the increasing number of HIV-infected children with mother to child transmission as the main route of infection. In 2010, the Ministry of Health created the recommended prophylaxis manual of mother to child HIV transmission and antiretroviral therapy in pregnant women and other behaviors related to prevention of mother to child HIV transmission. The objective of this study was to analyze if the recommendations of the Ministry of Health as control of mother to child HIV transmission are being taken by pregnant women and health professionals of a public maternity hospital in Goiânia. Research conducted between March and May 2012, divided into two stages: cross-sectional study, which consisted in the analysis of medical records of 323 HIV positive pregnant women seen at a public hospital in the State of Goiás between years 2006 to 2011, and second it is cross-sectional study involving 25 professionals working in maternity and obstetric center of this institution. Among the 323 records analyzed, 48.9% of pregnant women were included in the age group between 25 to 34 years old, 68.7% were single, 65.6% brown, 50.5% had study time between 4-7 years and 74.9% were unemployed. We checked the main risks related to mother to child HIV transmission in the 323 analyzed records. Among of the professionals surveyed, 76% have inadequate knowledge about HIV testing in pregnant women, 80% unaware the gestational age in what is realized elective caesarean, 66% do not know the dose to attack AZT and the elapsed time before child-birth, 84% do not know which method is indicated for lactation inhibition. The data observed in our study are alarming and show gaps in care for HIV positive pregnant women and newborns exposed, through information obtained that contradict this practice. / Nos últimos anos houve uma mudança do perfil da epidemia do HIV/aids, chamado por feminização da epidemia de HIV, um número crescente de mulheres em todo o mundo estão infectadas, sendo que mulheres e meninas constituem quase metade de todas as pessoas que vivem com vírus. A feminização da epidemia têm numerosas consequências, dentre elas, o aumento do número de crianças infectadas pelo HIV tendo a transmissão vertical como principal via de infecção. Em 2010, o Ministério da saúde criou o manual de Recomendações de profilaxia de transmissão vertical do HIV e terapia antirretroviral em gestantes e as demais condutas relacionadas a profilaxia da transmissão vertical do HIV. Assim, o objetivo geral deste estudo foi analisar se as medidas recomendadas pelo Ministério da Saúde como controle da transmissão vertical do HIV estão sendo adotadas por gestantes e profissionais de saúde de uma maternidade pública de Goiânia, Goiás. Pesquisa realizada entre março a maio de 2012, dividida em duas etapas: a primeira trata-se de estudo transversal, que consistiu na análise de 323 prontuários de gestantes HIV positivo atendidas em uma maternidade pública do Estado de Goiás entre os anos de 2006 a 2011, e a segunda trata-se de um estudo transversal envolvendo 25 profissionais atuantes na Maternidade e Centro Obstétrico desta Instituição. Dentre o 323 prontuários analisados, 48,9% das gestantes estavam inseridas na faixa etária entre 25 a 34 anos de idade, 68,7% eram solteiras, 65,6% pardas, 50,5% possuíam tempo de estudo entre 4 a 7 anos e 74,9% não possuíam vínculo empregatício. Foram verificados os principais riscos relacionados à transmissão vertical do HIV nos 323 prontuários analisados. Dos profissionais investigados 76% apresentam conhecimento inadequado sobre o teste anti-HIV na gestantes, 80% desconhecem com que idade gestacional realiza-se a cesárea eletiva, 66% não sabem a dose para ataque do AZT e o tempo utilizado antes do parto, 84% não conhecem qual o método indicado para inibição da lactação. Os dados observados em nosso estudo são preocupantes e evidenciam lacunas existentes na assistência prestada às gestantes HIV positivos e RN expostos, através de informações obtidas que contradizem essa prática.

Les troubles du comportement en sommeil paradoxal (TCSP) ont été individualisés comme un type de parasomnie par Carlos Schenck en 1986. Il s’agit d’une parasomnie caractérisée par l’absence d’atonie musculaire pendant le sommeil paradoxal associée à l’apparition de mouvements anormaux allant de simples secousses à des comportements élaborés souvent violents. Ces comportements dépendent de l’activité onirique et sont décrits comme une « mise en acte des rêves ». Les TCSP sont fréquents dans la maladie de Parkinson. Les patients parkinsoniens ayant des TCSP ont des troubles cognitifs plus importants et ont un risque de démence plus important que les autres patients. Les TCSP peuvent également se présenter de manière isolée, sans aucune cause pathologique apparente. Ils sont alors considérés comme « idiopathiques » (TCSPi). Ce terme « idiopathique » a néanmoins été remis en cause du fait de la présence de nombreux signes évocateurs d’une synucléinopathie chez ces patients (affaiblissement des performances cognitives, anomalies cérébrales structurales et fonctionnelles). De nombreux patients ayant des TCSP initialement considérés comme idiopathiques, vont développer un syndrome parkinsonien et/ou une démence après plusieurs années d’évolution. Une étude récente rapporte que cela concerne 81 % des patients après 16 ans de suivi. Ces données ont conduit à l’hypothèse selon laquelle les TCSP seraient un symptôme révélant les stades précoces « non-moteurs » de la MP. Les agrégats pathologiques d’alpha-synucléine touchant les structures du tronc cérébral seraient à l’origine de l’apparition de TCSP avant même le syndrome parkinsonien. Cette chronologie dans les stades d’évolution de la MP (pré-clinique, pré-moteur, moteur, démence) ne semble néanmoins pas être retrouvée chez tous les patients parkinsoniens. Elle révèlerait plutôt un certain sous-type de MP, caractérisée par de nombreux symptômes non-moteurs précédant le syndrome parkinsonien et par un risque accru de démence précoce. Parmi ces symptômes non moteurs, les troubles cognitifs seraient importants et toucheraient notamment le fonctionnement visuo-perceptif. Ce travail a eu pour objectif d’étudier le lien entre les TCSP, les troubles visuo-perceptifs et la maladie de Parkinson. Dans une première étude, des évaluations spécifiques des fonctions visuo-spatiales ont permis de mettre en évidence des troubles visuo-perceptifs chez les patients ayant des TCSPi. Les patients parkinsoniens ayant des TCSP avaient des troubles visuo-perceptifs plus marqués que ceux n’ayant pas de TCSP. Les résultats ont également permis d’identifier la nature des troubles visuo-perceptifs. Les processus intermédiaires de traitement de l’information visuelle semblent spécifiquement touchés dans ces groupes de patients.La deuxième partie de notre travail a été consacrée à l’exploration des corrélats neurophysiologiques de cette atteinte perceptive chez les patients ayant des TCSPi. Cette étude était basée sur l’analyse d’un potentiel évoqué cognitif : la Ncl (negativity associated with closure) décrite comme un marqueur de notre capacité à reconnaître des objets partiellement occultés ; autrement dit, une composante générée par la mise en jeu des processus intermédiaires de traitement de l’information visuelle. L’absence de Ncl observée chez les patients ayant des TCSPi est compatible avec l’hypothèse d’un dysfonctionnement des régions du Lateral occipital complex (LOC) situées sur la voie ventrale de traitement de l’information visuelle. [...] / Rapid eye movement sleep behavior disorder (RBD) has been recognized formally as a parasomnia in 1986 by Carlos Schenck. RBD is characterized by loss of normal muscle atonia during rapid eye movement sleep, associated with motor activity from simple jerks to elaborate, often violent, behaviors. This motor activity occurs while dreaming, and has been described as “acting out dreams”. RBD are frequent in Parkinson’s disease (PD). PD patients with RBD show more severe cognitive disorders and have a higher risk of developing dementia than patients without RBD. When isolated and with no identified pathological etiology, RBD are considered as “idiopathic” (iRBD). This “idiopathic” form has been questioned due to the presence of markers of neurodegeneration (cognitive disorders, structural and functional cerebral abnormalities) in iRBD patients. A quite large number of iRBD patients will eventually develop parkinsonism and/or dementia after several years. A recent study reports a delayed emergence of parkinsonism/dementia in 81 % of iRBD patients with a mean interval of 14 years from onset of RBD. These data has leaded to consider RBD as a symptom of the PD “pre-motor” stage. This classic progression of PD staging (pre-clinic, pre-motor, motor, dementia) is not found in every PD patient. It would rather reveal a specific PD sub-type characterized by many non-motor symptoms preceding parkinsonism and with a higher risk of early dementia. Cognitive disorders would be one of these non-motor symptoms, especially affecting visual perception. The goal of this doctoral dissertation was to study the relationship between RBD, visual perception disorders and PD. The first study aimed at identifying the nature of visual perception disorders in iRBD patients, based on specific assessments of visuo-spatial functions. The results showed a deficit of the intermediate stage of visual perception in iRBD patients. PD patients had a similar deficit which was more severe when associated with RBD. The second study focused on the analysis of the possible cerebral dysfunction underlying this visual perception deficit in iRBD patients. This study was based on the recording of the Ncl (negativity associated with closure), a cognitive event-related potential indexing the ability to recognize an object even partially occulted, mainly based on the intermediate stage of object processing. The results showed no Ncl component in iRBD patients. This is in line with a cerebral dysfunction located in the lateral occipital complex (LOC), a region belonging to the ventral visual pathway. The third study examined the cognitive profile of treatment-naïve PD patients, first at the time of diagnosis and then one year later. Previous published data demonstrated that the presence of RBD is associated with more severe cognitive disorders in treated PD patients. Based on the first assessments of our group of treatment-naïve patients, we examined whether these differences already exist at the time of diagnosis. The objective of the second evaluation session was to determine whether PD patients with RBD had a higher risk of early severe cognitive impairments than non-RBD patients. At the first session, 17 of the 57 patients (30%) met the criteria for RBD. At that time, RBD was not found to be associated with cognitive decline. We did not find clinical differences related to PD or others sleep disorders between RBD and non-RBD PD patients. Some yet isolated results might reflect a trend towards a potential decline in RBD patients. At the second session, only RBD patients but not non-RBD patients showed a cognitive slowing. RBD patients may also have a trend toward apathy. A longer follow-up study is needed to confirm this trend. [...]

Submitted by TIAGO SILVA DA FONSECA (tiago.odonto@hotmail.com) on 2018-08-15T13:52:28Z No. of bitstreams: 1 Tese - Tiago Silva da Fonseca.pdf: 3116289 bytes, checksum: 5bc17261ff2e5cf870764bac9df08bbb (MD5) / Approved for entry into archive by Ana Cristina Jorge null (anacris@fclar.unesp.br) on 2018-08-15T17:23:42Z (GMT) No. of bitstreams: 1 Fonseca_ts_dr_arafo_int.pdf: 3077264 bytes, checksum: 45ffe1976b230616d3be8df487229be6 (MD5) / Made available in DSpace on 2018-08-15T17:23:42Z (GMT). No. of bitstreams: 1 Fonseca_ts_dr_arafo_int.pdf: 3077264 bytes, checksum: 45ffe1976b230616d3be8df487229be6 (MD5) Previous issue date: 2018-03-28 / Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) / Biodentine é um cimento reparador à base de silicato tricálcico com indicações semelhantes ao MTA. O objetivo deste estudo foi avaliar a resposta do periodonto ao selamento de perfurações de furca em molares de ratos com Biodentine ou MTA e as possíveis alterações séricas em marcadores de atividade hepática e renal. As perfurações realizadas nos primeiros molares da maxila direita de 60 ratos foram preenchidas com Biodentine, MTA ou algodão (Sham). As maxilas contralaterais do grupo Sham foram utilizadas como grupo Controle. Após 7, 15, 30 e 60 dias, o sangue foi coletado e as maxilas foram fixadas e incluídas em parafina (n=5). A partir do soro foram mensurados ou níveis de transaminase glutâmico-oxalacética (TGO), transaminase glutâmico-pirúvica (TGP), ureia e creatinina. O sangue de 5 animais sem qualquer procedimento foi coletado para mensurar os padrões sorológicos de referência. O espaço periodontal (EP), densidade de volume de células inflamatórias (VvCI) e de fibroblastos (VvFb) e o número de osteoclastos TRAP-positivos foram obtidos. Interleucina-6 (IL-6) e osterix foram detectados por imuno-histoquímica. O conteúdo de colágeno birrefringente foi quantificado a partir de cortes corados com picrosirius. Os dados foram submetidos à ANOVA e ao teste Tukey (p≤0,05). Diferenças significantes nos níveis séricos de TGO (p≥0,1258) e TGP (p≥0,5827) não foram detectadas nos grupos Biodentine, MTA e Sham em comparação aos níveis de referência. Aos 7 dias, a concentração de ureia sérica foi significantemente maior nos grupos Biodentine e MTA em comparação aos níveis de referência (p=0,0166). Um nível mais alto de creatinina sérica no grupo Biodentine aos 60 dias foi detectado em comparação aos valores de referência (p=0,0222). Aos 7 dias, os altos valores de VvCI, células IL-6-positivas e número de osteoclastos foram acompanhados por reduzido conteúdo de colágeno no EP alargado dos grupos experimentais. Em todos os períodos, VvCI, número de osteoclastos e de células IL-6-positivas e EP foram significantemente maiores em Sham que nos grupos Biodentine e MTA (p<0,0001). Do 7º ao 60º dia, significante redução de VvCI, expressão de IL-6 e osteoclastos foi acompanhada por significante aumento de VvFb, osteoblastos e conteúdo de colágeno nos grupos Biodentine e MTA. Aos 60 dias, diferenças significantes em VvCI, espessura do LP, imunoexpressão de IL-6 e número de osteoclastos e osteoblastos não foram detectadas entre Biodentine e MTA (p≥0,3255). Aos 30 e 60 dias, o número de osteoblastos osterix-positivos foi significantemente mais alto nos grupos Biodentine e MTA que no grupo controle (p<0,0001) enquanto diferenças significantes no número de osteoclastos não foram observadas (p≥0,8933). A análise bioquímica sugere que Biodentine e MTA não induzem mudanças hepáticas no decorrer do tempo. No entanto, é possível que mudanças renais sejam promovidas por Biodentine. Apesar da reação inflamatória e reabsorção óssea iniciais, o selamento de perfurações de furca com Biodentine e MTA promovem condições para o reparo periodontal. / Biodentine is a calcium silicate-based cement with similar indications of MTA. The aim of this study was to evaluate the periodontium response in the sealing of furcation perforations in rat molars with Biodentine and MTA, and its potential systemic effects in serum levels of hepatic and renal functions. The pulp chamber floor of right upper first molars of 60 rats were perforated and filled with Biodentine, MTA or sterile cotton pellet (Sham); the left first molars were used as control. After 7, 15, 30 and 60 days, the blood was collected and serum glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), creatinine and urea levels were measured. The blood of 5 rats without any treatment was collected to measure serum reference levels. The maxillary fragments were fixed and processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and of fibroblasts (VvFb), and number of TRAP-positive osteoclasts were obtained. Interleukin-6 (IL-6), a pro-inflammatory cytokine, and osterix, an osteoblast marker, were detected by immunohistochemistry. The birefringent collagen content was quantified from picrosirius-stained sections. Data were submitted to ANOVA and Tukey test (p≤0.05). Significant differences in GOT (p≥0.1258) and GPT (p≥0.5827) serum levels were not observed in the Biodentine, MTA and Sham groups in comparison with reference levels. At 7 days, the concentration of serum urea increased significantly in the Biodentine and MTA groups compared with reference levels (p=0.0166). A higher concentration of serum creatinine in Biodentine group than reference level (p=0.0222) was detected at 60 days. At 7 days, the high values in VvIC, IL-6-immunolabelled cells and number of osteoclasts were accompanied by reduced collagen content in the enlarged PS of experimental groups. In all periods, VvIC, number of osteoclasts and IL-6, and PS were significantly higher in Sham than Biodentine and MTA groups (p<0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression and osteoclasts was accompanied by significant increase in VvFb, osteoblasts and collagen content in Biodentine and MTA groups. At 60 days, significant differences in VvIC, width of PS, IL-6 immunoexpression, in the number of osteoclasts and osteoblasts were not found between Biodentine and MTA (p≥0.3255). At 30 and 60 days, the number of osterix-immunolabelled osteoblasts was significantly higher in Biodentine and MTA than control group (p<0.0001) while significant differences in the number of osteoclasts were not observed (p≥0.8933). The biochemical analyses suggest that Biodentine and MTA did not induce changes in the liver over time. However, it is possible that kidney changes may be promoted by Biodentine. Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine or MTA provide conditions to the periodontium repair. / FAPEAM 117/2014

Orientador: Paulo Sérgio Cerri / Resumo: Biodentine é um cimento reparador à base de silicato tricálcico com indicações semelhantes ao MTA. O objetivo deste estudo foi avaliar a resposta do periodonto ao selamento de perfurações de furca em molares de ratos com Biodentine ou MTA e as possíveis alterações séricas em marcadores de atividade hepática e renal. As perfurações realizadas nos primeiros molares da maxila direita de 60 ratos foram preenchidas com Biodentine, MTA ou algodão (Sham). As maxilas contralaterais do grupo Sham foram utilizadas como grupo Controle. Após 7, 15, 30 e 60 dias, o sangue foi coletado e as maxilas foram fixadas e incluídas em parafina (n=5). A partir do soro foram mensurados ou níveis de transaminase glutâmico-oxalacética (TGO), transaminase glutâmico-pirúvica (TGP), ureia e creatinina. O sangue de 5 animais sem qualquer procedimento foi coletado para mensurar os padrões sorológicos de referência. O espaço periodontal (EP), densidade de volume de células inflamatórias (VvCI) e de fibroblastos (VvFb) e o número de osteoclastos TRAP-positivos foram obtidos. Interleucina-6 (IL-6) e osterix foram detectados por imuno-histoquímica. O conteúdo de colágeno birrefringente foi quantificado a partir de cortes corados com picrosirius. Os dados foram submetidos à ANOVA e ao teste Tukey (p≤0,05). Diferenças significantes nos níveis séricos de TGO (p≥0,1258) e TGP (p≥0,5827) não foram detectadas nos grupos Biodentine, MTA e Sham em comparação aos níveis de referência. Aos 7 dias, a concentração de urei... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Biodentine is a calcium silicate-based cement with similar indications of MTA. The aim of this study was to evaluate the periodontium response in the sealing of furcation perforations in rat molars with Biodentine and MTA, and its potential systemic effects in serum levels of hepatic and renal functions. The pulp chamber floor of right upper first molars of 60 rats were perforated and filled with Biodentine, MTA or sterile cotton pellet (Sham); the left first molars were used as control. After 7, 15, 30 and 60 days, the blood was collected and serum glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), creatinine and urea levels were measured. The blood of 5 rats without any treatment was collected to measure serum reference levels. The maxillary fragments were fixed and processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and of fibroblasts (VvFb), and number of TRAP-positive osteoclasts were obtained. Interleukin-6 (IL-6), a pro-inflammatory cytokine, and osterix, an osteoblast marker, were detected by immunohistochemistry. The birefringent collagen content was quantified from picrosirius-stained sections. Data were submitted to ANOVA and Tukey test (p≤0.05). Significant differences in GOT (p≥0.1258) and GPT (p≥0.5827) serum levels were not observed in the Biodentine, MTA and Sham groups in comparison with reference levels. At 7 days, the concentration of serum urea increased significantly in the B... (Complete abstract click electronic access below) / Doutor

Made available in DSpace on 2016-12-08T16:44:42Z (GMT). No. of bitstreams: 1 PGPV10MA102.pdf: 27178752 bytes, checksum: 39b01ef3a94b25575ab96d69f1cc7b69 (MD5) Previous issue date: 2010-11-05 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / On 2008/09 and 2009/10 seasons, 21 bean materials were evaluated, in wich, four of them were commercial cultivation Pérola, SCS 202 Guará, IPR Uirapuru and |BRS Supremo and 17 were accesses from active germoplasma bean ban of Santa Catarina State University, BAF/UDESC, Lages. Two experiments were done. One experiment was realized in field of UDESC, where were made plant anthracnose incidence, analyzing 10 plants in random, on the two central lines of each parcel; leaflet incidence: collecting inside of the two central lines of each parcel, 60 leaflet lesions number evaluations: counting number of lesions by leaflet. The second experiment was realized at the plant pathology laboratory of UDESC, where the Colletotrichum lindemuthianum incidence were evaluated in three different seed sanity tests, in wich two culture environment BDA (potato-dextrose-agar) and V8 (tomato juice) and a Blotter Test detection method, where the 200 seeds lots of each material evaluated were disposed in gerbox boxes containing the respective tests, in a repetition of a 50 seeds by box to each experiment proportion. On field evaluations, 7 BAF accesses showed themselves more promising in relation to the commercial cultivation tested, showing better resistance, in which BAF s 02, 10 and 14 first experiment year and BAF s 07, 45, 60 and 148, in second experiment year. In seed sanity tests, the V8 media showed the best seed pathogen detection method and 7 accesses showed a biggest seed anthracnose incidence, related to the tested commercial cultivation. In 2008/09 season, the cultivar SCS 202 Guará presented a bigger seed anthracnose incidence, differing significantly of all tested material. In 2009/10 season, the BAF 07 showed a bigger seed anthracnose incidence, followed by cultivar IPR Uirapuru and BAF s 02, 10, 45 and 60. It s concluded that there are inside BAF/UDESC promising material showing resistance to anthracnose disease and the C. lindemuthianum was detected with more sensibility in V8 media / Nas safras 2008/09 e 2009/10, foram avaliados 21 materiais de feijão, sendo que destes, quatro cultivares comerciais: Pérola, SCS 202 Guará, IPR Uirapuru e BRS supremo e 17 acessos do banco Ativo de Germoplasma de Feijão da Universidade do Estado de Santa Catarina (BAF/UDESC), Lages, SC. Foram conduzidos dois experimentos, sendo um deles na área experimental da UDESC, onde foram feitas avaliações de incidência de antracnose em plantas, analisando-se 10 plantas aleatoriamente, nas duas linhas centrais de cada parcela; incidência em folíolos, coletando-se dentro das duas linhas centrais de cada parcela, 50 folíolos de número de lesões por folíolos; contando número de lesões por folíolo. Um segundo experimento foi realizado no laboratório de fitopatologia da UDESC, onde se quantificou a incidência de colletotrichum lindemuthianum em três diferentes testes de sanidade de sementes, sendo dois meios de cultura organizados BDA (Batata-Dextrose-Ágar) e V8 (Suco de tomate) e o método de detecção Blotter Test. Foram plaqueadas 2º sementes de cada acesso em caixas de gerbox contendo os respectivos testes, sendo quatro repetições de 50 sementes. Nas avaliações de campo, sete acessos do BAF foram mais promissores em relação às cultivares comerciais testadas, no que diz respeito à maior resistência, sendo os BAF s 02, 10 e 14 em 2008/09 e os BAF s 07, 45, 60 e 148, em 2009/10. Nos testes de sanidade de sementes o meio V8 foi o melhor método de detecção do patógeno. Sete acessos mostraram maior incidência de C. lindemuthianum em sementes em relação às cultivares comerciais testadas. Na safra 2008/09 a cultivar SCS 202 Guará apresentou maior incidência do patógeno em sementes, diferindo significativamente de todos os materiais testados. Em 2009/10 o BAF 07 mostrou maior incidência do fungo em sementes, seguidos da cultivar IPR Uirapuru e dos BAF s 02, 10, 45 e 60. Conclui-se que existem no BAF/UDESC materiais promissores quanto à reação à antracnose e que o fungo C. lindemuthianum é detectado com maior sensibilidade em sementes de feijão no meio de cultura V8