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[ES] El empleo de organismos vivos como biofactorías ha ganado una atención significativa en la industria debido a la creciente demanda de sistemas de producción sostenibles y la escasez de recursos. Entre sus muchas aplicaciones, las biofactorías pueden ser diseñadas para producir feromonas de insectos, las cuales sirven como alternativa ecológica a los pesticidas para el control de plagas en la agricultura. Como prueba de concepto, en esta tesis doctoral se caracterizaron plantas de Nicotiana benthamiana modificadas genéticamente con una ruta multigénica para producir las feromonas de polillas (Z)-11-hexadecenol (Z11-16OH) y (Z)-11-hexadecenil acetato (Z11-16OAc). Las plantas resultantes produjeron cantidades moderadas de ambas feromonas (111.4 µg g-1 FW y 11.8 µg g-1 FW para Z11-16OH y Z11-16OAc, respectivamente), y tasas de emisión diarias de ~10 ng g-1 FW para cada feromona. La producción de feromonas afectó negativamente al desarrollo de las plantas, probablemente debido a la sustancial carga metabólica y posible toxicidad de estos productos. Una estrategia para superar estas limitaciones es diseñar un sistema de expresión condicional que permita a las plantas crecer con normalidad antes de inducir la producción de feromonas. Para ello desarrollamos un conjunto de promotores sintéticos personalizables, llamados GB_SynP, activables con dCasEV2.1, un activador transcripcional potente y programable desarrollado recientemente para la inducción de genes en plantas. Los promotores GB_SynP permitieron una regulación precisa de los transgenes, con unos niveles de transcripción robustos y modulables en el estado "encendido" (presencia de dCasEV2.1 y la correspondiente guía de ARN), y una expresión mínima en el estado "apagado". Para implementar el sistema de producción condicional de feromonas en plantas se generó una nueva ruta multigénica para la biosíntesis de feromonas de polilla bajo el control de los promotores GB_SynP. Paralelamente, el activador dCasEV2.1 se reguló transcripcionalmente mediante el módulo CUP2:GAL4 sensible a sulfato de cobre, un inductor químico ampliamente utilizado en la agricultura. La funcionalidad del sistema se probó mediante expresión transitoria en N. benthamiana, resultando en unos rendimientos en el estado "encendido" de 32.7 µg g-1 FW y 25 µg g-1 FW para Z11-16OH y Z11-16OAc, respectivamente, y unos niveles insignificantes en ausencia de cobre. Sin embargo, la expresión en estable de esta ruta en N. benthamiana produjo unos niveles de expresión de los transgenes significativamente menores y una marcada disminución en la producción de feromonas. Esto supone que el sistema en su forma actual resulte inviable como biofactoría de feromonas en términos prácticos. La optimización de este sistema debe centrarse en mejorar la cascada de activación, en el uso de especies de plantas alternativas con mayor biomasa, y/o en incrementar las tasas de emisión en planta. Como alternativa a la producción de feromonas en plantas, la intercambiabilidad de piezas génicas entre plantas y hongos filamentosos puede aprovecharse para crear biofactorías fúngicas de feromonas. En este sentido, nuestro grupo adaptó previamente el sistema GoldenBraid a hongos filamentosos, llamado FungalBraid. En esta tesis ampliamos la colección de FungalBraid incorporando 27 piezas nuevas que incluyen diferentes marcadores de selección y promotores constitutivos e inducibles, los cuales se caracterizaron funcionalmente en Penicillium digitatum y P. chrysogenum. Además, se expresaron con éxito los promotores GB_SynP en P. digitatum, en combinación con el sistema de dCas9 activadora contenido en el vector pAMA18. Aunque los niveles de expresión de GB_SynP en hongos filamentosos fueron menores que los observados previamente en plantas, ésta y otras herramientas disponibles en la colección FungalBraid pueden utilizarse en el futuro para el desarrollo de biofactorías fúngicas que produzcan feromonas de insectos y otras biomoléculas de alto valor. / [CA] L'ús d'organismes vius com biofàbriques ha guanyat una atenció significativa a la indústria a causa de la creixent demanda de sistemes de producció sostenible i l'escassetat de recursos. Entre les seues moltes aplicacions, les biofàbriques poden ser dissenyades per a produir feromones d'insectes, les quals serveixen com a alternativa ecològica als pesticides per al control de plagues a l'agricultura. Com a prova d'aquest concepte, en aquesta tesi doctoral es van caracteritzar plantes de Nicotiana benthamiana modificades genèticament plantes de amb una ruta multigènica per a produir les feromones d'arnes (Z)-11-hexadecenol (Z11-16OH) i (Z)-11-hexadecenil acetat (Z11-16OAc). Les plantes resultants van produir quantitats moderades de totes dues feromones (111.4 µg g-1 FW i 11.8 µg g-1 FW per a Z11-16OH i Z11-16OAc, respectivament), i taxes d'emissió diàries d'aproximadament 10 ng g-1 FW per a cada feromona. La producció de feromones en aquestes plantes va afectar negativament el seu desenvolupament, probablement a causa de la substancial càrrega metabòlica i possible toxicitat d'aquests productes. Una estratègia per superar aquestes limitacions és dissenyar un sistema d'expressió condicional que permeta a les plantes créixer amb normalitat abans d'induir la producció de feromones. Per això hem desenvolupat un conjunt de promotors sintètics personalitzables, anomenats GB_SynP, activables amb dCasEV2.1, un activador transcripcional potent i programable desenvolupat recentment per a la inducció de gens en plantes. Els promotors GB_SynP van permetre una regulació precisa des transgens, amb uns nivells de transcripció robustos i modulables a l'estat "encès" (presència de dCasEV2.1 i la corresponent guia d'ARN), i una expressió mínima a l'estat "apagat". Per implementar el sistema de producció condicional de feromones en plantes es va generar una nova ruta multigènica per a la biosíntesi de feromones d'arna sota el control dels promotors GB_SynP. Paral·lelament, l'activador dCasEV2.1 es va regular transcripcionalment al mòdul CUP2:GAL4 sensible al sulfat de coure, un inductor químic àmpliament utilitzat en l'agricultura. La funcionalitat del sistema es va provar mitjançant expressió transitòria en N. benthamiana, resultant en uns rendiments a l'estat "encès" de 32.7 g-1 FW i 25 µg g-1 FW per a Z11-16OH i Z11-16OAc, respectivament, i uns nivells insignificants en absència de coure. No obstant això, l'expressió estable d'aquesta ruta a N. benthamiana va produir uns nivells d'expressió dels transgens significativament menors i una marcada disminució en la producció de feromones. Això suposa que el sistema en la seua forma actual resulte inviable com a biofàbrica de feromones en termes pràctics. L'optimització d'aquest sistema ha de centrar-se en millorar la cascada d'activació, en l'ús d'espècies de plantes alternatives amb major biomassa, i/o en incrementar les taxes d'emissió a la planta. Com a alternativa a la producció de feromones en plantes, la intercanviabilitat de peces gèniques entre plantes i fongs filamentosos pot aprofitar-se per crear biofàbriques fúngiques de feromones. En aquest sentit, el nostre grup va adaptar prèviament el sistema GoldenBraid a fongs filamentosos, anomenat FungalBraid. En aquesta tesi, vam ampliar la col·lecció de FungalBraid incorporant 27 peces noves que inclouen diferents marcadors de selecció i promotors constitutius i induïbles, els quals es van caracteritzar funcionalment a Penicillium digitatum i P. chrysogenum. A més, es van expressar amb èxit els promotors GB_SynP en P. digitatum, en combinació amb el sistema de dCas9 activadora contingut en el vector pAMA18. Encara que els nivells d'expressió de GB_SynP en fongs filamentosos van ser menors que els observats prèviament en plantes, aquesta i altres eines disponibles a la col·lecció FungalBraid poden utilitzar-se en el futur per al desenvolupament de biofàbriques fúngiques que produeixin feromones d'insectes i altres biomolècules de gran valor. / [EN] The use of living organisms as biofactories have gained significant attention in the industry due to the increasing demand for sustainable production systems and the shortage of resources. Among their many applications, biofactories can be engineered to produce insect pheromones, which serve as eco-friendly alternatives to pesticides for pest management in agriculture. As a proof of concept, in this thesis we characterized Nicotiana benthamiana plants engineered with a multigene pathway to produce the moth pheromones (Z)-11-hexadecenol (Z11-16OH) and (Z)-11-hexadecenyl acetate (Z11-16OAc). The resulting transgenic plants produced modest amounts of both pheromones (111.4 µg g-1 FW and 11.8 µg g-1 FW for Z11-16OH and Z11-16OAc, respectively), and daily emission rates of ~10 ng g-1 FW for each pheromone. Pheromone production in these plants significantly affected their fitness, likely due to the substantial metabolic burden and possible toxicity of lipid-derived products. One strategy to address these developmental abnormalities consists of engineering conditional transgene expression systems, thus allowing plants to grow normally before inducing the production of pheromones. To achieve this goal, in this thesis we developed a set of customizable synthetic promoters called GB_SynP, which can be activated by dCasEV2.1, a strong programable transcriptional activator recently developed for plant gene regulation. These GB_SynP promoters enabled tight regulation of single and multiple transgenes, with robust and tunable transcription levels in the ON state (presence of dCasEV2.1 loaded with the corresponding gRNA), and minimal or undetectable expression in the OFF state. To implement a conditional expression system for pheromone production in plants, a newly engineered multigene pathway for the biosynthesis of moth pheromones was constructed under the control of GB_SynP promoters. In parallel, the dCasEV2.1 activator was transcriptionally regulated with the CUP2:GAL4 sensor for copper sulphate, an agronomically-compatible chemical trigger. The functionality of this system was tested transiently in N. benthamiana, resulting in estimated yields of 32.7 µg g-1 FW and 25 µg g-1 FW for Z11-16OH and Z11-16OAc respectively in the ON state, and negligible levels in the absence of copper. However, stable transformation of the same copper-regulated pheromone pathway in N. benthamiana plants resulted in significantly lower transgene expression levels, which translated into a great reduction of pheromone yields. This makes the system in its current form a non-viable pheromone biofactory in practical terms. Further optimization should focus on the improvement of the activation cascade, the use of alternative plant hosts with more biomass, and/or the enhancement of emission rates in planta. As an alternative to pheromone production in plants, the interchangeability of DNA parts between plants and filamentous fungi could also be exploited to create fungal biofactories for pheromone production. In this regard, our research group previously adapted the GoldenBraid system for filamentous fungi, which we named FungalBraid. In this thesis, we expanded the FungalBraid collection by incorporating 27 new DNA parts, including different selection markers and several constitutive and inducible promoters, all of which were functionally characterized in Penicillium digitatum and P. chrysogenum. Furthermore, we successfully expressed the GB_SynP promoters developed for plants in P. digitatum, in combination with the non-integrative pAMA18-derived vector for the expression of a dCas9-based activator. Although further optimization of GB_SynP in filamentous fungi is required, as expression levels were lower than those previously observed in plants, this and the other tools available in the FungalBraid collection can be effectively employed in the future for the development of fungal biofactories that produce insect pheromones and other high value biomolecules. / Este trabajo ha sido financiado mediante la Ayuda para la Formación de Profesorado Universitario FPU18/02019 (Ministerio de Educación, Cultura y Deporte), así como por el proyecto europeo SUSPHIRE (PCI2018-092893, Era- CoBiotech), y los proyectos de Plan Nacional I+D PID2019-108203RB-100 y PID2021-125858OB-100 (Ministerio de Ciencia e Innovación). / Moreno Giménez, E. (2023). Synthetic biology tools for production of insect pheromones in plants and filamentous fungi [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/201180

Depuis le vingtième siècle, les flux migratoires ont augmenté entrainant des flux financiers – nommés "remittances" - partant des zones d'immigration vers celles d'émigration. Ces transferts, que les migrants envoient à leurs familles dans leur pays d'origine peuvent générer des effets opposés selon des pays. Cette thèse a pour objectif d'analyser leurs conséquences sur le capital physique et humain dans les pays qui les reçoivent.Le premier chapitre souligne l'impact négatif de ces flux sur le stock de capital ainsi que sur l'offre de travail. Ce revenu supplémentaire durant leur dernière période de vie conduit les individus à moins travailler et moins épargner. Ces transferts peuvent rapprocher ou éloigner les économies de la règle d'or d'accumulation du capital en fonction de leurs caractéristiques. Une politique de taxation est proposée afin de maximiser le bien-être. Le second chapitre étend l'analyse à des économies ouvertes faisant face à des contraintes exogènes de crédit sur le marché international des capitaux. Il montre que les transferts réduisent les incitations des agents à épargner, mais peuvent accroitre l'investissement en capital physique dans certains pays, via les entrées de capitaux, en relâchant directement la contrainte de crédit. Le troisième chapitre développe un modèle de croissance avec accumulation de capital humain, dans lequel les agents empruntent pour s'éduquer. Les contraintes de crédit sont cette fois endogènes : les agents ne peuvent s'engager à rembourser et sont exclus du marché des capitaux en cas de défaut. Ce modèle explique que les "remittances" peuvent générer des effets positifs, dans certains pays, ou négatifs dans d'autres. / Since the twentieth century, migratory flows have largely increased entailing financial flows - named remittances - from immigration areas to emigration areas. These transfers that migrants send to their families in their home countries may empirically lead to positive or negative effects on physical and human capital. This thesis aims at explaining this mixed evidence in developing countries.The first chapter underlines the negative impact of remittances on capital stock but also on labour supply. Due to this additional income in their last-period of life, agents have less incentive to work and save. Remittances may bring economies closer to their golden rule of capital accumulation or further from their golden rule according to their features. A taxation policy is provided in order to maximise the welfare in the home country of the migrants. The second chapter extends the analysis to open economies facing exogenous credit constraints on the international capital market. Even if remittances reduce incentive to save, these flows may increase investment in physical capital in some countries, through capital inflows, by directly relaxing the credit constraints. The third chapter considers a growth model with human capital accumulation in which agents borrow to finance their education. Borrowing constraints are, in this non-commitment framework considered as endogenous: agents may choose to default and are excluded from the financial market in case of default. In accordance with empirical literature, this model is able to explain a negative or a positive impact of remittances on economic growth.

Este trabalho tem como objetivo propor um diálogo entre a leitura do Quixote em contexto escolar e os estudos literários, por meio da análise do processo de transferência da obra canônica de Miguel de Cervantes para o sistema literário infantil. Apontamos, neste percurso, as principais questões, enfrentadas tanto por adaptadores da obra quanto por educadores, no que tange à complexa tarefa de colocar a obra ao alcance dos leitores infantojuvenis. Esta pesquisa permite discutir aspectos textuais, históricos e culturais das adaptações, além de sugerir uma abordagem prática da introdução do Quixote em contexto escolar que estimule o empoderamento do leitor infantojuvenil, resgatando seu papel de leitor ativo na própria narrativa cervantina. Palavras-chave: Dom Quixote de la Mancha, Escolarização da leitura, Ensino de espanhol, Adaptação, Literatura infantojuvenil, Formação do leitor, Empoderamento do leitor, Literatura espanhola século de ouro, Miguel de Cervantes (1547-1616), Recepção de Quixote / This research aims to propose a dialogue between the reading of Quixote in the school context and the literary studies, analyzing it by the process of transferring the Miguel de Cervantes canonical book to the childrens literary system. We focus on the main issues, faced by adapters and educators, in the complex task of providing the work to the childrens readers. This thesis allows us to discuss textual, historical and cultural aspects of the adaptations, as well as it suggests a practical approach to use of the Quixote in the school context, stimulating the empowerment of the childrens reader, and resignifying their active role in the Cervantes narrative.

O Quadrilátero Ferrífero (MG) é uma importante unidade geotectônica do Cráton do São Francisco que alberga jazidas de ferro e ouro hospedadas na seqüência greenstone belt Rio das Velhas e cuja evolução geodinâmica esteve regida por vários eventos tectonometamórficos. Depósitos de ouro gerados por fluidos epitermais e remobilizações orogênicas proterozóicas se associam intimamente com camadas bandas de ferro e raramente disseminados em metapelitos no topo do greenstone. Nas mineralizações é comum uma paragênese que cristalizou mediante diversos processos e em distintos estágios com predomínio de sulfetos de ferro. Em geral os minérios possuem forte controle estrutural por dobras, falhas e cisalhamentos transcorrentes, desenvolvidos durante vários eventos deformacionais de diversa índole e estreita relação com processos de alteração hidrotermal como sericitização, carbonatação, cloritização, entre outros de menor incidência. O depósito de ouro Córrego do Sítio hospeda-se na seqüência silisiclástica de origem turbidítica metamorfisada em fácies xisto verde denominada Associação de Litofácies Ressedimentada que faz parte do Grupo Nova Lima, se apresenta disseminado em metapelitos e em veios de quartzo com carbonato. A mineralização que acompanha o ouro cristalizou em vários estágios principais nos que predominaram (i) pirita + pirrotita, (ii) arsenopirita + pirrotita + pirita com texturas finas e orientadas, (iii) arsenopirita com pirrotita e sulfosais em veios de quartzo, (iv) pirita grossa pervasiva em todas as rochas hospedeiras. O estudo da composição isotópica Pb, Sr e Nd, permitiu identificar que as fontes dos fluidos mineralizantes foram as rochas hospedeiras, que na evolução policíclica da mineralização participaram eventos metamórficos e hidrotermais a 2.2, 2.0 Ga. e sobreimpostos a ~600 Ma. os quais induziram os processos de circulação hidrotermal na área de estudo. Mediante comparativa com outros depósitos da região, sugere-se que os metais formadores do minério depositaram-se em Córrego do Sítio à idade de 2.2 Ga. originados a partir da BIF tipo São Bento e remobilizados por fluidos através de falhas inversas. Análises geocronológicas U-Pb (SHRIMP) e K-Ar embora não decisivos em quanto à determinação da idade da mineralização, revelaram a idade de ~2.7 Ga. como o limite temporal para a deposição das rochas hospedeiras. No contexto geológico aplicado se estabelece a potencialidade para a prospecção aurífera que apresentam as rochas metapelíticas do topo do Greenstone Belt Rio das Velas associadas a camadas bandadas de ferro com ouro, e que os alvos mais relevantes seriam zonas com falhas inversas e cisalhamentos transcorrentes. / The Quadrilátero Ferrífero is an important geotectonic unit of the São Francisco Craton that host iron and gold deposits within the vulcanosedimentary rocks of the greenstone belt Rio das Velhas. The geodynamic evolution of this greenstone includes several tectonometamorphic events. Gold deposits were formed by epithermal fluids, remobilized during the Proterozoic orogenic processes, are associated whit the banded iron formations and are sporadically disseminate in metapelitic rocks within the top of the greenstone sequence. In the ores is common the crystallization of iron sulfide minerals during diverse stages. Gold deposits have an important structural control by folds, reverse faults and strike-slip shearing zones, and are commons several hydrothermal alteration processes like sericitization, chloritization and carbonate usually related to the deformation. The Córrego do Sítio gold deposit is hosted in turbiditic siliciclastic rocks that are metamorphosed in the greenschist facies. The gold is associated with the iron sulfide arsenopyrite and pyrite, which are disseminated in metapelitic rocks and quartz-carbonate veins. The mineralization includes vary stages of crystallization, (1) pyrite and pyrrotite (2) arsenopyrite, pyrrotite and fine pyrite, (3) arsenopyrite whit pyrrotite and sulphosalts in quartz vein, and (4) pervasive pyrite. An integrated Pb, Sr e Nd, isotopic study of the Córrego do Sítio deposit allowed to identify that sources of the mineralizing fluids was the host rocks, that in the policiclic evolution the gold mineralizaton had participation events of metamorphism and hidrothermalism to age 2.2, 2.0 and 0.6 Ga. respectively the wich induced the hydrothermal circulation processes in the study area. The isotopic character of this deposit and its comparison with similar ones, suggest that the gold and its metals associated it is deposited in Córrego do Sítio to age 2.2 Ga. and that metals were derived from the banded iron formations, and remobilized by hidrothermal fluids across reverse faults. Although U-Pb (SHRIMP) and K-Ar geochronologic data were not conclusive to constrain the age of the mineralization, they provide to age ~2.7 Ga. that a good limit for the sedimentation of the host rock. It is suggest that there is a potential for gold prospection within the metapellitic rocks associated with banded iron formation of the Greenstone Rio das Velhas, and that the more relevant targets include areas with inverse and strike-slip fault.

This thesis discusses contemporary Scottish crime fiction in light of its transatlantic contexts. It argues that, despite participating in a globalized popular genre, examples of Scottish crime fiction nevertheless meaningfully intervene in notions of Scottishness. The first chapter examines Scottish appropriations of the hard-boiled mode in the work of William McIlvanney, Ian Rankin, and Irvine Welsh, using their representation of traditional masculinity as an index for wider concerns about community, class, and violence. The second chapter examines examples of Scottish crime fiction that exploit the baroque aesthetics of gothic and noir fiction as a means of dealing with the same socio-political contexts. It argues that the work of Iain Banks and Louise Welsh draws upon a tradition of distinctively Scottish gothic in order to articulate concerns about the re-incursion of barbarism within contemporary civilized societies. The third chapter examines the parodic, carnivalesque aspects of contemporary Scottish crime fiction in the work of Christopher Brookmyre and Allan Guthrie. It argues that the structure of parody replicates the structure of genre, meaning that the parodic examples dramatize the textual processes at work in more central examples of Scottish crime fiction. The fourth chapter focuses on examples of Scottish crime fiction that participate in the culturally English golden-age and soft-boiled traditions. Unpacking the darker, more ambivalent aspects of these apparently cosy and genteel traditions, this final chapter argues that the novels of M. C. Beaton and Kate Atkinson obliquely refract the particularly Scottish concerns about modernity that the more central examples more openly express.

Cette thèse présente une édition philologique et annotée du roman peu connu, écrit et publié par le maître d’escrime de Philippe IV, Luis Pacheco de Narváez, dont le titre est Historia ejemplar de las dos constantes mujeres españolas, imprimé pour la première fois à Madrid par l’Imprenta del Reino l’année 1635. Le travail est divisé en cinq chapitres, puisque en plus de celui consacré à l’édition du roman (chap. V), qui établit un texte annoté basé sur les deux seules éditions connues (Madrid, 1635 et Séville, 1744) avec son catalogue de variantes, quatre autres lui servent de contexte. Ainsi, nous présentons une biographie de Pacheco de Narváez (Baeza, 1560-Madrid, 1640), rédigée autour de la documentation conservée, qui est accompagnée par une liste de références littéraires d’époque qui évoquent sa figure et son œuvre, et une épigraphe qui se veut une réflexion scientifique sur ses relations avec Francisco de Quevedo (chap. I); une histoire du texte qui étudie la date de composition du roman, la première édition et le moment compliqué pour publier des romans dans ces années à cause de la censure (1625-1635), d’un projet de publication non abouti et de la seconde édition, ainsi que la trajectoire critique du roman et l’absence d’éditions ultérieures (chap. II); une étude de nature taxonomique (bien que l’accent soit mis aussi sur la moralité présente dans le roman) qui implique son rattachement, en dépit de son extension, au genre du roman «courtois» (chap. III); et enfin un chapitre dédié au contexte littéraire, divisé en deux grands blocs: l’un consacré aux sources et aux matériaux que l’auteur a utilisés pour donner forme au roman, et un autre qui étudie, à titre d’exemple, l’un des sujets présent dans ce dérnier et qui était aussi très courant dans la littérature de l’époque: la descriptio tempestatis. Cette dernière partie est complétée par un appendice qui analyse le sujet de la tempête dans la Jerusalén conquistada (1609) de Lope de Vega (chap. IV). / This thesis present a critical edition of the non-well known novel written and published by the fencing master of Philip IV, Luis Pacheco de Narváez, whose title is Historia ejemplar de las dos constants mujeres españolas, printed for the first time in Madrid by the Imprenta del Reino the year 1635. The work is divided into five chapters. In addition to the section dedicated to the edition of the novel (ch. V), which establishes an annotated text based on the only two known editions (Madrid, 1635 and Seville, 1744) with her list of variants, another four serve as context to it. Thus, we present a biography of Pacheco de Narváez (Baeza, 1560-Madrid, 1640), drafted in accordance with the preserved documentation, which is complemented by a literary list of references of this period about the figure and the work of the author, and an epigraph that wants to be a scientific consideration on his relations with Francisco de Quevedo (ch. I); a history of the text that studies the date of composition, the first edition and the complicated situation to publish novels in these years due to censorship (1625-1635), an aborted publication project and the second edition, as well as the critical trajectory of the novel and the absence of later editions (ch. II); a taxonomic study (although emphasis is placed on the morality present in the story) that implies its attachment to the genre of the so-called “courtly novel” (ch. III); and finally a chapter dedicated to the literary context, divided in two large parts: one dedicated to the sources and materials that the author could use to form the novel, and another that studies, by way of example, one of the literary topics present in the text and in the literature of the time: the descriptio tempestatis. This last part complements, as addition, with a study of the subject of the storm in the Jerusalen conquistada (1609) by Lope de Vega (ch. IV). / El objeto de esta tesis es presentar una edición filológica y anotada de la poco conocida novela escrita y publicada por el maestro de esgrima del monarca Felipe IV, Luis Pacheco de Narváez, cuyo título es Historia ejemplar de las dos constantes mujeres españolas, impresa por vez primera en Madrid por la Imprenta del Reino el año de 1635. El trabajo se divide en cinco capítulos, pues además del específico dedicado a la edición de la obra (Cap. V), que establece un texto anotado sobre la base de las dos únicas ediciones conocidas (Madrid, 1635 y Sevilla, 1744) junto con su aparato de variantes, otros cuatro sirven de contexto a la misma. Así, presentamos una nueva y completa biografía de Luis Pacheco de Narváez (Baeza, ha. 1560-Madrid, 1640), redactada en torno a la documentación conservada, que se complementa con un importante elenco de las menciones literarias de época que hacen referencia a la figura y la obra del autor, y un epígrafe que quiere ser una reflexión científica sobre sus relaciones con el escritor Francisco de Quevedo (Cap. I); una historia del texto que estudia la fecha de composición de la obra, la primera edición madrileña de 1635 y la complicada tesitura para publicar novelas en estos años a causa de la censura imperante (1625-1635), un proyecto de publicación abortado y la segunda edición sevillana de 1744, así como la trayectoria crítica de la novela y la inexistencia de ediciones posteriores (Cap. II); un estudio de carácter taxonómico (aunque se haga hincapié en la moralidad presente en el relato) que supone su adscripción al género, pese a su extensión, de la denominada como novela «cortesana», bien que se constate y analice también la presencia de elementos de otros géneros como la novela bizantina, caballeresca, etc. (Cap. III); y por último un capítulo dedicado al contexto literario, dividido a su vez en dos grandes bloques: uno dedicado a las fuentes y materiales que pudo utilizar el autor para conformar la novela, y otro que estudia, a manera de ejemplo, uno de los tópicos literarios presentes en la misma y en la literatura de la época: la descriptio tempestatis. Este último bloque se complementa, en tanto que apéndice, con un estudio del tema de la tormenta en el libro VII de La Jerusalén conquistada (1609) de Félix Lope de Vega (Cap. IV). Se cierra la tesis con el consabido apartado bibliográfico.

This research examines the career of Robert Searle, an English privateer, that conducted state-sponsored attacks against the Spanish and Dutch in the Caribbean from 1655 to 1671. Set within the Buccaneering Period of the Golden Age of Piracy (1650-1680), Robert Searle’s personal actions contributed to the rise of the English in the Caribbean to a position of dominance over Spain, which dominated the region from 1492 until the 1670s. Searle serves as a window into the contributions of thousands of nameless men who journeyed to the Caribbean as a member of Oliver Cromwell’s Western Design Fleet. These men failed in their endeavor to take Hispaniola from the Spanish, successfully invaded Jamaica, and spent the next fifteen years securing England’s largest possession in the region, transitioning Jamaica from a military outpost to a successful plantation colony. These men, including Searle himself, have been overshadowed in the history of English Jamaica by more well-known figures such as Sir Henry Morgan, the famed “Admiral of the Buccaneers.” Searle and his compatriots pursued the objectives of the core in London throughout the contested periphery of the Caribbean region. These goals were first framed as the complete destruction of the Spanish Empire in the Americas and later as achieving trade between Jamaica and Spain’s American colonies. The examination of Robert Searle through the core-periphery relationship between the metropole and the Caribbean illustrates how the totality of his actions contributed to the rising English position in the Caribbean. Ultimately, Searle and his fellow privateers proved vital to Spain conceding to England the rights of trade and formal recognition of their colonies in the region with a series of succeeding Treaties of Madrid.

One major lesson derived from the implementation of the Endangered Species Act (ESA) over the past 30 years is that direct regulation is not the only nor the optimal way to protect endangered species on working lands because of an undue burden imposed on private landowners. The role of a voluntary conservation program is to rearrange incentives so that society bears the cost rather than the landowner. Employing a survey research methodology, I used theories of reasoned action and random choice to explore landowners? stated preferences for conservation programs. I found landowners? stated interest in compensation programs to be moderate at best. For those willing to consider programs involving endangered species, associating land management requirements for species conservation with direct benefits to the landowner is important, but perhaps not as important as ensuring that the program provides adequate financial incentives, consideration of the term of the program, and a level of certainty regarding the landowner?s future obligations under the ESA. Landowners are not a homogenous group. I identified two classes of landowners according to preferences for program structure. One group was highly sensitive to program structure, aside from financial incentives, while the other was likely to participate if adequately compensated with financial and technical assistance. These differences related to opinions on endangered species protection and dependence on their land for income. Voluntary incentive programs increasingly are a popular tool to maintain and enhance conservation; however, these programs are only successful insofar as landowners choose to enroll. This research demonstrates that improving recovery efforts on private lands requires program administrators to have a more complete understanding of landowners? views on endangered species and conservation programs in general, as well as their motivations for owning and operating their land. By doing so, programs with broader appeal and greater efficacy can be designed and implemented.

Bound Together elucidates how gay and lesbian leather communities, in the years between 1966 and 1984, contested and expanded fungible notions of sex, community, and history, mostly through material and visual cultural systems: dress codes such as the hanky code, architectural spaces (bars, bathhouses, private clubs), garments, posters, advertisements, newsletters, films, and performances. In examining visual and material cultures, procedures of archival research, as well as the physical states of key archives associated with historic gay and lesbian leather communities, this dissertation opens out a discussion of a set of visual documents and terms rarely considered within the discipline of art history, or academia at large. Through rigorous rhetorical experimentation Bound Together seeks to propose new ways of writing histories. Long and short chapters are interpolated, telescoping between historical leather communities and key works of contemporary art which reformat 1970s documents and visual sources. Jean Luc-Nancy’s conception of “being-with,” a state of coterminous existence that lies at the foundation of being and subjecthood, provides an ideal framework for coming to terms with the challenges of writing leather histories. Nancy’s notion is one that privileges mutual and relational difference. The structure of Bound Together works similarly, building a set of differential modes of viewing, analyzing and writing. In this way I wish to, in the words of Tilottama Rajan, use “history as the condition for an internal distanciation and for self-reflection on what we do,” and to furthermore present alternatives to a discipline’s often “routinized, even commodified […] repeatable techniques.” / text