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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
61

N?o t?m fim os amores sem volta : di?logos entre verdades e fic??es

Roca, Andr? Lu?s Nunes 23 January 2017 (has links)
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T13:09:25Z No. of bitstreams: 1 DIS_ANDRE_LUIS_NUNES_ROCA_PARCIAL.pdf: 2835869 bytes, checksum: e4ea198fcc1cb0af873dd86d690d99e0 (MD5) / Made available in DSpace on 2017-06-30T13:09:25Z (GMT). No. of bitstreams: 1 DIS_ANDRE_LUIS_NUNES_ROCA_PARCIAL.pdf: 2835869 bytes, checksum: e4ea198fcc1cb0af873dd86d690d99e0 (MD5) Previous issue date: 2017-01-23 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / This paper is divided in two parts. The first one follows a theoretical line, in which issues inherent to biographical construction are analyzed, such as the capacity to fill the blanks in someone?s life, understanding that there is only one way: the truth. In contrast, it also presents a line where fiction is considered the base necessary to fill these spaces in an existence, creating a real effect, a truth possibility. Still within the reflective essay, questions about the literary doing (the artistic process) are approached. The other part of the work is a novel, titled N?o t?m fim os amores sem volta. It is a story about a researcher who commands the exhumation of the first Brazil emperors' while goes deep in an intimate path that will lead her to an understanding about her own life. / O presente trabalho est? dividido em duas partes distintas, mas complementares. A primeira segue uma linha te?rica, em que s?o analisadas quest?es inerentes ? constru??o biogr?fica, como a capacidade de preencher as lacunas de uma vida, por vezes clamando para si o papel de basti?o da Hist?ria, de defensora de uma verdade absoluta. Em contrapartida, apresenta-se uma linha que se utiliza de fic??o para retratar as possibilidades de uma exist?ncia, criando n?o uma verdade, mas um efeito de real. Em seguida, ainda dentro do ensaio reflexivo, s?o abordadas quest?es acerca do fazer liter?rio e sobre o processo de produ??o art?stica que envolve esta disserta??o. A outra parte do trabalho ? uma novela ficcional, intitulada N?o t?m fim os amores sem volta. Nela, uma pesquisadora comanda os trabalhos de exuma??o dos restos mortais dos primeiros imperadores do Brasil enquanto percorre um caminho ?ntimo que a levar? a um entendimento sobre a pr?pria vida.
62

Participa??o do c?rtex insular na forma??o da mem?ria de reconhecimento social

Cavalcante, Lorena Evelyn Silva 10 March 2017 (has links)
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:39:27Z No. of bitstreams: 1 DIS_LORENA_EVELYN_SILVA_CAVALCANTE_PARCIAL.pdf: 843706 bytes, checksum: 9e28b91e2d08f5204a5e3b2293c8a063 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:39:35Z (GMT) No. of bitstreams: 1 DIS_LORENA_EVELYN_SILVA_CAVALCANTE_PARCIAL.pdf: 843706 bytes, checksum: 9e28b91e2d08f5204a5e3b2293c8a063 (MD5) / Made available in DSpace on 2017-06-30T18:39:41Z (GMT). No. of bitstreams: 1 DIS_LORENA_EVELYN_SILVA_CAVALCANTE_PARCIAL.pdf: 843706 bytes, checksum: 9e28b91e2d08f5204a5e3b2293c8a063 (MD5) Previous issue date: 2017-03-10 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The insular cortex (IC) receives afferent projections from prefrontal cortex, cingulate gyrus, olfactory bulb, basal nuclei and forms reciprocal connections with important limbic areas: amygdala and entorhinal cortex. These different connections indicate a possible involvement of the insular cortex in the process of learning and memory. Social recognition memory (SRM) is essential for forming social groups, establishing hierarchies and social and affective ties. Despite its importance, the knowledge about the brain structures and the cellular and molecular mechanisms involved in its processing is still scarce. Thus, the present study aimed to verify the participation of NMDA-glutamatergic, D1/D5-dopaminergic, H2-histaminergic, ?-adrenergic and 5-HT1A serotoninergic receptors, of the IC, in the consolidation of SRM. For this, male Wistar adult rats (300-330 g) were submitted to stereotaxic surgery for implantation of bilateral cannulae in the IC and, later, to the task of social discrimination. The task consists of 4 consecutive days of habituation to the experimental apparatus, which is an open-field box containing 2 acrilic cilinders, for 20 min. Twenty four hours after the last habituation session, the animals were placed in the open field in the presence of a juvenile (22 days postnatal) for 1 hour (sample phase). After 24 hours, the retention test occurred, for 5 min, in the presence of a juvenile previously met (familiar) and a new juvenile. Animals that received the intra-IC infusion of the antagonist D1/D5, ?-adrenergic or 5-HT1A receptor, immediately after the sample phase, impairs the consolidation of SRM. However, this effect was blocked by the concomitant intra-IC infusion of the agonist and the antagonist of the respective receptors. In addition, animals that received intra-IC infusion of the antagonists NMDA and H2 receptors, immediately after the sample phase, were able to consolidate the SRM. The results obtained in the present study suggest that the dopaminergic D1/D5, ?-adrenergic and serotonergic 5-HT1A receptors, but not the glutamatergic NMDA and the histaminergic H2 receptors, of the IC, participates in the consolidation of the SRM. / O c?rtex insular (CI) recebe proje??es aferentes do c?rtex pr?-frontal, giro cingulado, bulbo olfat?rio, n?cleos da base, al?m de formar conex?es rec?procas com importantes ?reas l?mbicas: am?gdala e c?rtex entorrinal. Estas diferentes conex?es indicam um poss?vel envolvimento do c?rtex insular no processo de aprendizado e mem?ria. A mem?ria de reconhecimento social (MRS) ? essencial para formar grupos sociais, estabelecer hierarquias e v?nculos sociais e afetivos. Apesar de sua import?ncia, ainda ? escasso o conhecimento sobre o papel das estruturas cerebrais e os mecanismos celulares e moleculares envolvidos em seu processamento e armazenamento. Assim, o presente trabalho teve como objetivo verificar a participa??o dos receptores glutamat?rgicos do tipo NMDA, dopamin?rgicos D1/D5, histamin?rgico H2, ?-adren?rgico e serotonin?rgico 5-HT1A, do c?rtex insular, no processo de consolida??o da MRS. Para isso, ratos Wistar machos adultos foram submetidos a uma cirurgia estereot?xica para implanta??o de c?nulas bilaterais no CI e, posteriormente, ? tarefa de discrimina??o social. Esta tarefa consiste de 4 sess?es di?rias de 20 min de habitua??o ao aparato experimental, que ? constitu?do de uma caixa de campo aberto contendo dois cilindros de acr?lico. Vinte e quatro horas ap?s a ?ltima sess?o de habitua??o os animais foram recolocados no aparato, na presen?a de um coespec?fico juvenil (22 dias p?s-natal) dentro de um dos cilindros, por 1 hora (sess?o de treino). Ap?s 24 horas os animais foram submetidos a uma sess?o de teste, de 5 min, na presen?a de um juvenil desconhecido e do juvenil previamente encontrado (familiar). Verificou-se que, os animais que receberam a infus?o intra-CI do antagonista dos receptores D1/D5, ?-adren?rgico ou 5-HT1A imediatamente ap?s a sess?o de treino, tiveram um preju?zo na consolida??o da MRS. Contudo, esse efeito foi bloqueado pela infus?o intra-CI concomitante do agonista e do antagonista dos respectivos receptores. Ainda, os animais que receberam a infus?o intra-CI do antagonista dos receptores NMDA ou H2 imediatamente ap?s a sess?o de treino, foram capazes de consolidar a MRS. Os resultados obtidos no presente trabalho sugerem que os receptores dopamin?rgicos D1/D5, ?-adren?rgicos e serotonin?rgicos 5-HT1A, mas n?o os glutamat?rgicos NMDA e os histamin?rgicos H2, do c?rtex insular participam da consolida??o da MRS.
63

Elimina??o segura de arquivos em mem?ria n?o-vol?til / Secure file deletion in non volatile memory

Weber, Julia Silva 21 March 2017 (has links)
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:43:22Z No. of bitstreams: 1 DIS_JULIA_SILVA_WEBER_COMPLETO.pdf: 1157200 bytes, checksum: 9480342a98027ac78d6f2fd1c5eb50d7 (MD5) / Made available in DSpace on 2017-06-30T18:43:22Z (GMT). No. of bitstreams: 1 DIS_JULIA_SILVA_WEBER_COMPLETO.pdf: 1157200 bytes, checksum: 9480342a98027ac78d6f2fd1c5eb50d7 (MD5) Previous issue date: 2017-03-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The advent of the Internet of Things (IoT) and the popularization of mobile devices with non-volatile memory brings new challenges regarding the removal of files. Techniques traditionally employed in magnetic media are not effective when applied to non-volatile memories, such as flash memory. Because of the peculiar characteristics of this type of memory, notably the existence of a Flash Translation Layer (FTL), operating systems only manage logical blocks, and no longer have direct control of the physical blocks of a flash memory. Consequently, new methods of safe removal have been developed, which employ Zero Override, Block Erase, and Cryptographic Erase techniques. This work analyzes these methods, compares their operations and proposes a new method, with better performance than those described in the literature. The proposed method is a hybrid method, which combines overwriting and deletion operations to obtain a balanced use of these operations, avoid unnecessary deletion of unused blocks and reduce premature memory wear. To verify the efficiency of the proposed method and of the other methods, a simulator was developed to exercise the removal of files in several experiments. / O advento da Internet das Coisas (IoT) e a populariza??o de dispositivos m?veis com mem?ria n?o-vol?til traz novos desafios quanto a remo??o de arquivos. T?cnicas tradicionalmente empregadas em meios magn?ticos n?o s?o efetivas quando aplicadas para mem?rias n?o vol?teis, como a mem?ria flash. Devido ?s caracter?sticas peculiares deste tipo de mem?ria, notadamente a exist?ncia de uma Camada de Tradu??o da Flash (FTL), sistemas operacionais somente gerenciam blocos l?gicos, e n?o tem mais controle direto dos blocos f?sicos de uma mem?ria flash. Consequentemente, novos m?todos de remo??o segura foram desenvolvidos, que empregam opera??es de Sobrescrita com Zeros, de Apagamento de Blocos e t?cnicas de Apagamento Criptogr?fico. Este trabalho analisa estes m?todos, compara suas opera??es e prop?e um novo m?todo, com melhor desempenho que os descritos na literatura. O m?todo proposto ? um m?todo h?brido, que combina de forma equilibrada opera??es de sobrescrita e apagamento, para evitar o apagamento desnecess?rio de blocos ainda n?o utilizados e reduzir o desgaste prematuro da mem?ria. Para verificar a efici?ncia do m?todo proposto e dos demais m?todos, foi desenvolvido um simulador para exercitar a remo??o de arquivos em diversos experimentos.
64

Comunica??o e mem?ria empresarial : os livros institucionais da Ipiranga - 1962 e 1997

Ordoque, Christian Astigarraga 30 March 2017 (has links)
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-07-05T13:51:22Z No. of bitstreams: 1 DIS_CHRISTIAN_ASTIGARRAGA_ORDOQUE_COMPLETO.pdf: 3323562 bytes, checksum: ce73fa468b2cffee94fac55821912e80 (MD5) / Made available in DSpace on 2017-07-05T13:51:23Z (GMT). No. of bitstreams: 1 DIS_CHRISTIAN_ASTIGARRAGA_ORDOQUE_COMPLETO.pdf: 3323562 bytes, checksum: ce73fa468b2cffee94fac55821912e80 (MD5) Previous issue date: 2017-03-30 / Institutional book is one of the communication products most traditionally used by companies to publicize their trajectory. Throughout them the companies elaborate a narrative that differentiate them from their competitors. In our study, we sought to analyze two institutional books: The first book is called ?Petrolinho Edi??o Especial Comemorativa ao 25? Anivers?rio da Refinaria de Petr?leo Ipiranga S/A?, produced in 1962. The second book of Ipiranga has the title: ?Uma Hist?ria de Realiza??es. Empresas Petr?leo Ipiranga 60 anos?, produced in 1997. Our analysis will be made through the Hermeneutics Depth method of John B. Thompson and we will use as Technical Discursive Textual Analysis of Roque Moraes, trying to answer if the books present the memory or the history of the company. For that, the analysis of three contexts is necessary; The relationship between culture and organizational communication, the debate between History and Memory and the more current context of corporate social responsibility and historical responsibility. With our study, we intend to contribute to the analysis of the insitutional book, its process of elaboration and use as an communication tool for companies. / O livro institucional ? um dos produtos de comunica??o mais tradicionalmente utilizados pelas empresas para a divulga??o de sua trajet?ria. Atrav?s deles as empresas elaboram uma narrativa que as diferenciam de seus concorrentes. Em nosso estudo, buscamos analisar dois livros institucionais: O primeiro livro se chama ?Petrolinho Edi??o Especial Comemorativa ao 25? Anivers?rio da Refinaria de Petr?leo Ipiranga S/A?, produzido em 1962. O segundo livro da Ipiranga tem o t?tulo: ?Uma Hist?ria de Realiza??es. Empresas Petr?leo Ipiranga 60 anos?, produzido em 1997. Nossa an?lise ser? efetuada atrav?s do m?todo Hermen?utica de Profundidade de John B. Thompson e utilizaremos como t?cnica a An?lise Textual Discursiva de Roque Moraes, buscando responder se os livros apresentam a mem?ria ou a hist?ria da empresa. Para tanto, a an?lise de tr?s contextos se faz necess?ria; a rela??o entre cultura e comunica??o organizacional, o debate entre Hist?ria e Mem?ria e o contexto mais atual da responsabilidade social empresarial e da responsabilidade hist?rica. Pretendemos com nosso estudo, contribuir para a an?lise do livro institucional, seu processo de elabora??o e utiliza??o como ferramenta de comunica??o para as empresas.
65

Sangue e sombras : a mem?ria familiar em Vermelho, de Mafalda Ivo Cruz

Canilha, Samla Borges 18 January 2018 (has links)
Submitted by PPG Letras (letraspg@pucrs.br) on 2018-03-07T13:16:35Z No. of bitstreams: 1 DISSERTA??O_SAMLA.pdf: 842743 bytes, checksum: ed530d173e5afdd37b1fde2d115ea526 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-03-08T14:42:56Z (GMT) No. of bitstreams: 1 DISSERTA??O_SAMLA.pdf: 842743 bytes, checksum: ed530d173e5afdd37b1fde2d115ea526 (MD5) / Made available in DSpace on 2018-03-08T14:46:50Z (GMT). No. of bitstreams: 1 DISSERTA??O_SAMLA.pdf: 842743 bytes, checksum: ed530d173e5afdd37b1fde2d115ea526 (MD5) Previous issue date: 2018-01-18 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The present study aims to analyse the use of memory, especially of family memory, in the novel Vermelho (2003), from the portuguese writer Mafalda Ivo Cruz. In it, the narrator protagonist, Tito, seeks to recover the genealogy of his family, resulting in a narrative marked by a form that largely reflects the discourse of memory, a theme dear to the author's work. Thus, the theoretical reference used here mainly consists of memory theorists such as Henri Bergson, Maurice Halbwachs, Paul Ricoeur, Aleida Assmann and J?el Candau. Besides that, we resorted to texts from narratology to support the reading of the novel, especially in what concerns the construction of the narrator. From the analysis performed, one can see that, contrary to what is expected of a memory narrative, that is, an organization of the past in a coherent and temporally linear whole, Tito transmits the family's past into a construction that reflects his disturbed mind. The chaos is also a reflection of the fact that the family history in evidence is full of gaps and silences that are often fictionally filled by its members, especially the manipulation elaborated by M?rio, the stepfather, the main responsible for the oral transmission of events to Tito. Ultimately, the objects of the family archive, in addition to being suspected of falsehood, may have been selected arbitrarily to serve the protagonist's purposes. Therefore, the narrator ends by, rather than reconstituting, founding a memory of the family, but the result is problematic, once Tito is not a reliable narrator. / Este trabalho tem como objetivo analisar o aproveitamento da mem?ria, especialmente da mem?ria familiar, no romance Vermelho (2003), da escritora portuguesa Mafalda Ivo Cruz. Nele, o protagonista narrador, Tito, procura recuperar a genealogia de sua fam?lia, do que resulta uma narrativa marcada por uma forma que reflete, em grande parte, o discurso da mem?ria, tema caro ? obra da autora. Sendo assim, o referencial te?rico utilizado consiste, principalmente, em te?ricos que tratam da mem?ria, tais como Henri Bergson, Maurice Halbwachs, Paul Ricoeur, Aleida Assmann e J?el Candau. Al?m disso, recorremos a textos da narratologia para embasar a leitura do romance, principalmente no que tange ? constru??o do narrador. Da an?lise realizada, pode-se perceber que, ao contr?rio do que se espera de uma narrativa de mem?ria, isto ?, uma organiza??o do passado em um todo coerente e temporalmente linear, Tito transmite o passado da fam?lia em uma constru??o que reflete sua mente perturbada. A caoticidade ? tamb?m reflexo do fato de a hist?ria familiar em jogo ser repleta de lacunas e de sil?ncios que s?o preenchidos n?o raro ficcionalmente pelos seus membros, destacando-se a manipula??o elaborada por M?rio, o padrasto, principal respons?vel pela transmiss?o oral dos eventos a Tito. Por fim, os objetos do arquivo familiar, al?m de serem suspeitos de falsidade, podem ter sido selecionados arbitrariamente para servir aos fins do protagonista. Assim, o narrador acaba por, mais que reconstituir, fundar uma mem?ria da fam?lia, mas o resultado ? problem?tico, uma vez que Tito n?o se mostra um narrador confi?vel.
66

Efeito do Piriproxifeno sobre o desenvolvimento, par?metros comportamentais e end?crinos em larvas e adultos de Peixe-zebra (Danio rerio)

Gusso, Darlan 21 February 2018 (has links)
Submitted by PPG Biologia Celular e Molecular (bcm@pucrs.br) on 2018-04-04T12:33:24Z No. of bitstreams: 1 DARLAN_GUSSO_DIS.pdf: 1507228 bytes, checksum: e2389f511956dbb64bb7b767d8f73e40 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-04-19T16:26:33Z (GMT) No. of bitstreams: 1 DARLAN_GUSSO_DIS.pdf: 1507228 bytes, checksum: e2389f511956dbb64bb7b767d8f73e40 (MD5) / Made available in DSpace on 2018-04-19T16:43:13Z (GMT). No. of bitstreams: 1 DARLAN_GUSSO_DIS.pdf: 1507228 bytes, checksum: e2389f511956dbb64bb7b767d8f73e40 (MD5) Previous issue date: 2018-02-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Mosquitoes are responsible for the transmission of various pathogens. Over the past three years, the world has witnessed epidemics affecting all age groups, such as dengue fever, yellow fever, chikungunya and zika virus. To control and eliminate Aedes vectors responsible for the transmission of these diseases, it is necessary to use larvicides and insecticides such as pyriproxyfen. Pyriproxyfen is a larvicide used to control mosquitoes, mainly of the genus Aedes. This study evaluated the effects of pyriproxyfen on survival, morphology, behavior, and endocrine parameters at different stages of zebrafish development. We have demonstrated that pyriproxyfen can cause changes in the survival rate, behavior and morphology of exposed larvae, adults exposed at the larval stage, and adult offspring of exposed parents. Larvae exposed to pyriproxyfen for 1-96 hpf (hours post-fertilization) showed a decrease in survival rate, heart rate 3 dpf (days post-fertilization), and body length at 5 and 8 dpf. The distance traveled and the mean velocities of the exposed larvae were reduced in comparison to the vehicle group (1% DMSO). Therefore, pyriproxyfen changes the morphology and behavior of zebrafish in early stages of development and may affect the next generations. However, when the exposed larvae were maintained until the adult stage (6 months), we did not observe differences in locomotor parameters. Parental exposure to pyriproxyfen induced decrease in locomotion of zebrafish adult offspring as well as an anxiolytic-like behavior. We also tested pyriproxyfen exposure for 96 hours in adult zebrafish and the locomotion, anxiety, memory and endocrine parameters were analyzed as well as the gene expression of the glucocorticoid receptor (GR) and corticotropin-releasing factor (CRF). These results showed that there was no significant difference in locomotion, anxiety and endocrine parameters. In addition, there was an impairment of habituation memory in adult zebrafish exposed to pyriproxyfen. Therefore, it is important to control the use of larvicides due to their toxic effects on non-target species. Our findings demonstrated the importance of studies related to the use of larvicides, since they are potential causes of morphological and behavioral alterations in aquatic species, such as zebrafish. / Mosquitos s?o respons?veis pela transmiss?o de v?rios agentes patog?nicos. Nos ?ltimos tr?s anos, o mundo testemunhou epidemias que afetam todas as faixas et?rias, tais como dengue, febre amarela, chikungunya e zika v?rus. Para controlar e eliminar vetores do g?nero Aedes, respons?vel pela transmiss?o destas doen?as, ? necess?rio usar larvicidas e inseticidas como o piriproxifeno. O piriproxifeno ? um larvicida usado para eliminar mosquitos, principalmente do g?nero Aedes. Este estudo avaliou os efeitos do piriproxifeno sobre a sobreviv?ncia, morfologia, comportamento e par?metros end?crinos em diferentes est?gios de desenvolvimento de peixe-zebra. N?s demonstramos que o piriproxifeno pode causar altera??es na taxa de sobreviv?ncia, comportamento e morfologia de larvas expostas, adultos expostos no est?gio larval e descendentes adultos de pais expostos. As larvas expostas ao piriproxifeno durante 1-96 hpf (horas p?s-fertiliza??o) mostraram uma diminui??o na taxa de sobreviv?ncia, frequ?ncia card?aca 3 dpf e comprimento corporal nos 5 e 8 dpf (dias p?s- fertiliza??o). A dist?ncia percorrida e a velocidade m?dia das larvas expostas foram reduzidas em rela??o ao grupo ve?culo (1% de DMSO). Portanto, o piriproxifeno altera a morfologia e o comportamento do peixe-zebra em est?gios iniciais de desenvolvimento e pode afetar as pr?ximas gera??es. No entanto, quando as larvas expostas foram mantidas at? o est?gio adulto (6 meses), n?o observamos diferen?as nos par?metros locomotores. A exposi??o parental ao piriproxifeno induziu uma diminui??o na locomo??o da prole adulta, bem como um comportamento do tipo ansiol?tico. N?s tamb?m avaliamos o efeito da exposi??o ao piriproxifeno em peixe-zebra na fase adulta durante 96 horas sobre a locomo??o, ansiedade, mem?ria e par?metros end?crinos, como express?o g?nica do receptor de glicocorticoide (GR) e fator de libera??o de corticotrofina (CRF). Os resultados demonstraram que n?o houve diferen?a significativa na locomo??o, ansiedade e par?metros end?crinos. Al?m disso, houve um preju?zo da mem?ria de habitua??o do peixe-zebra adulto exposto ? piriproxifeno. Portanto, ? importante controlar o uso de larvicidas devido aos seus efeitos t?xicos em esp?cies n?o-alvo. Nossos achados demonstram a import?ncia de estudos relacionados com a utiliza??o de larvicidas, uma vez que s?o potenciais causadores de altera??es morfol?gicas e comportamentais em esp?cies aqu?ticas, como o peixe-zebra.
67

Mem?ria de reconhecimento e localiza??o de objetos em peixe-zebra (Danio rerio) : influ?ncia da sinaliza??o glutamat?rgica e respostas end?crinas

Gaspary, Karina Vidarte 08 February 2018 (has links)
Submitted by PPG Biologia Celular e Molecular (bcm@pucrs.br) on 2018-04-24T12:44:41Z No. of bitstreams: 1 KARINA_VIDARTE_GASPARY_DIS.pdf: 1111674 bytes, checksum: e2b2dc4b1b1eea8b10007b7c07f592ec (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-05-11T17:57:10Z (GMT) No. of bitstreams: 1 KARINA_VIDARTE_GASPARY_DIS.pdf: 1111674 bytes, checksum: e2b2dc4b1b1eea8b10007b7c07f592ec (MD5) / Made available in DSpace on 2018-05-11T18:03:07Z (GMT). No. of bitstreams: 1 KARINA_VIDARTE_GASPARY_DIS.pdf: 1111674 bytes, checksum: e2b2dc4b1b1eea8b10007b7c07f592ec (MD5) Previous issue date: 2018-02-08 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Memory and learning allow animals to adapt and modify their behavior towards new experiences. Some factors may result in memory deficits such as: neurodegenerative diseases, changes due to intoxication and the use of drugs. Zebrafish can be used to model complex human behavioral traits such as learning and memory. This study aims to establish a protocol for evaluating the object recognition memory or object location tasks in zebrafish. We evaluated novel object recognition memory and analyzed the exploration time of novel and familiar objects in the training and test sessions. There was a preference of the animal to explore the new object in comparison to the familiar object (61% of exploration time). We also evaluated the object location task and measured the exploration time of each object in the familiar and novel object locations. There was a preference of the animal to explore the object in the novel location in comparison to the object in the familiar location (63% of exploration time). We also evaluated the effect of the non-competitive NMDA receptor antagonist MK-801 on the object recognition and object location memory in zebrafish. In this regimen treatment, control (water only) and treated animals (5 ?M MK-801) presented a significant preference in exploring the familiar object in comparison to the new object (66 and 68% of exploration time, respectively); however, 10 ?M MK-801-treated animals did not show differences in the exploration time of the objects. In the object location task, the animals treated with the 5 or 10 ?M MK-801 did not show a significant preference for the familiar or novel location whereas the control group had a higher preference in exploring the object in the familiar location (64% of exploration time). Therefore, it is possible to suggest that 5 ?M MK-801 impaired the memory formation in an object location task, which is in agreement with previous studies demonstrating the cognitive deficit induced by MK-801 treatment in aversive and spatial memory. Considering the different responses of the control group between original task and in the regimen treatment, we evaluated the impact of habituation on cortisol levels of animals in three different protocols: 1) habituated at the experiment apparatus for 3 days (Condition 1 ? C1), 2) habituated at the experiment apparatus for 3 days plus treatment tank exposure at fourth day (Condition 2 - C2), 3) habituated at the treatment tank exposure and experiment apparatus for 3 days and exposed to treatment tank again at fourth day (Condition 3 ? C3). The results showed higher levels of cortisol in animals submitted to C2 and C3 conditions compared to animals submitted to C1. These results demonstrated that the animals submitted to treatment tank exposure have a different performance in object recognition and object location memory due to stress responses. Therefore, these tasks are prone to evaluate memory in physiological and pathological conditions, but its use is limited to perform pharmacological studies due to sensitivity to stress caused by manipulation. / A mem?ria permite que os animais possam adaptar e modificar seu comportamento diante de novas experi?ncias. Uma s?rie de fatores pode resultar num d?ficit de mem?ria como: doen?as neurodegenerativas, altera??es decorrentes de intoxica??es e uso de f?rmacos. O peixe-zebra vem sendo usado para modelar caracter?sticas comportamentais humanas complexas, como o aprendizado e mem?ria. Este estudo tem como objetivo estabelecer um protocolo para avaliar tarefas de mem?ria de reconhecimento de objetos ou de localiza??o de objetos no peixe-zebra. N?s avaliamos a mem?ria de reconhecimento de objeto novo e analisamos o tempo de explora??o de objetos novos e familiares nas sess?es de treino e teste. Houve uma prefer?ncia do animal em explorar o novo objeto em compara??o com o objeto familiar (61% do tempo de explora??o). Tamb?m avaliamos a tarefa de localiza??o de objeto e medimos o tempo de explora??o de cada objeto nos locais familiares e novos. Houve uma prefer?ncia do animal para explorar o objeto na localiza??o nova em compara??o com o objeto na localiza??o familiar (63% do tempo de explora??o). Tamb?m avaliamos o efeito do antagonista n?o competitivo do receptor NMDA MK-801 na mem?ria de reconhecimento de objeto novo e na localiza??o de objeto em peixe-zebra. Neste tratamento, o controle (somente ?gua) e os animais tratados (MK-801 5 ?M) apresentaram uma prefer?ncia significativa na explora??o do objeto familiar em compara??o com o novo objeto (66 e 68% do tempo de explora??o, respectivamente); no entanto, animais tratados com MK-801 10 ?M n?o mostraram diferen?as no tempo de explora??o dos objetos. Na tarefa de localiza??o de objeto, os animais tratados com MK-801 5 ou 10 ?M n?o mostraram prefer?ncia significativa pelo objeto na localiza??o familiar ou nova, enquanto que o grupo controle teve uma prefer?ncia maior em explorar o objeto na localiza??o familiar (64% de tempo de explora??o). Portanto, sugere-se que o MK-801 5 ?M prejudicou a forma??o da mem?ria em uma tarefa de localiza??o de objeto, o que est? de acordo com estudos pr?vios, demonstrando o d?ficit cognitivo induzido pelo MK-801 em mem?rias aversivas ou espaciais. Considerando as diferentes respostas do grupo controle entre a tarefa original e no tratamento, avaliamos o impacto da habitua??o nos n?veis de cortisol dos animais em tr?s protocolos diferentes: 1) habituado ao aparato experimental por 3 dias (Condi??o 1 - C1) 2) habituado ao aparato experimental por 3 dias mais a exposi??o ao aqu?rio de tratamento no quarto dia (Condi??o 2 - C2), 3) habituado na exposi??o ao aqu?rio de tratamento e no aparato experimental por 3 dias e exposto ao aqu?rio de tratamento novamente no quarto dia (Condi??o 3 - C3). Os resultados mostraram n?veis mais elevados de cortisol em animais submetidos a condi??es C2 e C3 em compara??o com animais submetidos a C1. Esses resultados demonstraram que os animais submetidos ? exposi??o ao aqu?rio de tratamento apresentam desempenho diferente nas tarefas de mem?ria de reconhecimento de objetos e de localiza??o do objeto devido ao estresse. Portanto, essas tarefas s?o adequadas para avaliar a mem?ria em condi??es fisiol?gicas e patol?gicas, mas seu uso ? limitado em estudos farmacol?gicos devido ? sensibilidade ao estresse causado pela manipula??o.
68

Uma hist?ria visual da luta pela terra : Porto Alegre, Pra?a da Matriz, 1990

Formolo, Deise 28 March 2018 (has links)
Submitted by PPG Hist?ria (historia-pg@pucrs.br) on 2018-05-24T16:24:53Z No. of bitstreams: 1 Uma hist?ria visual impresso para entrega.pdf: 7032655 bytes, checksum: ebe88cc273ab1eee029aabd48596793a (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-06-04T12:59:30Z (GMT) No. of bitstreams: 1 Uma hist?ria visual impresso para entrega.pdf: 7032655 bytes, checksum: ebe88cc273ab1eee029aabd48596793a (MD5) / Made available in DSpace on 2018-06-04T13:12:25Z (GMT). No. of bitstreams: 1 Uma hist?ria visual impresso para entrega.pdf: 7032655 bytes, checksum: ebe88cc273ab1eee029aabd48596793a (MD5) Previous issue date: 2018-03-28 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / This research aimed to understand the construction of the visual memory of the event of the struggle for land that took place in Pra?a da Matriz, in the city of Porto Alegre, on August 8, 1990. To do so, we sought to build series based on the analysis and interpretation of how the event was constructed, given to see and conveyed by the major regional and national commercial press. In dialogue, a series of eighty-six photographs from different institutional and private collections, entitled SINDJORS series, were analyzed. The dissertation was structured in five chapters. The first chapter is configured in the introduction, where the theoretical basis of the dissertation is presented, based on the concepts of journalistic field (Bourdieu), visuals (Menezes), public photography (Mauad), memory (Candau) and survival (Didi-Huberman). In the second chapter, an immersion was carried out in the context of the construction of the photograph produced in Brazil between the 1960s and 1990s. Emphasis was placed on the professional career of photographers, whose production of images is linked to the land issue. This route was traced to the photographers of the SINDJORS series. Then, aspects of the historical course of the rural social movements in Rio Grande do Sul (Harres), (Eckert) were presented. It was analyzed how the construction of the photographic practices (Mauad) of these professionals, dedicated to the production of images on the question of land, is related, in some aspects, to the very construction of the struggle for land. In chapters three and four, considering the pressures of the market (economic capital) and political on the field of journalism, we identified some strategies adopted by the commercial communication vehicles to report the event, which can be summarized in these points: criminalize the MST act; disqualify the political action, directing the agenda for the death of the soldier Valdeci de Abreu and decontextualizing the history of the struggle for land; and construct the narratives from the idea of ?conflict?. Finally, it was observed that the idea of "confrontation" was present in the relationship between the photographs and the text published in the periodicals. The photographs published in the newspapers analyzed complain of a report of denunciation of police violence, in opposition to the text that tends to value the action of the military police. In chapter five, the series of images of SINDJORS was analyzed, identifying their patterns and survivals, as well as the editions of the labor union newspapers (SINJORS and Sindibanc?rios) and of the newspaper Sem Terra (MST). It was found that the discursive elaboration of the newspaper "Sem Terra? has similarities with the versions reported by the Jornal do SINDJORS and O Banc?rio. However, in "Sem Terra", there is an accentuation on the concern to insert the event of Pra?a da Matriz in the prism of the historical course of the struggle for land in Brazil, highlighting the violent scenario led by the governments to deal with the land issue. It can be said that the construction of the visual memories of the event are shaped by the dispute between the versions of the journalistic text, condensed in repetition and reproduction, and the visualities of photographs, based on the tension between resistance and repression, and in the survivals of images of other temporalities. / Esta pesquisa teve como objetivo compreender a constru??o da mem?ria visual do acontecimento da luta pela terra ocorrido na Pra?a da Matriz, na cidade de Porto Alegre, no dia oito de agosto de 1990. Para tal, buscou-se construir s?ries a partir da an?lise e interpreta??o de como o acontecimento foi constru?do, dado a ver e veiculado pela grande imprensa comercial regional e nacional. Em di?logo, analisou-se uma s?rie de oitenta e seis fotografias provenientes de diferentes acervos institucionais e privados, intitulada neste trabalho de s?rie do SINDJORS. A disserta??o foi estruturada em cinco cap?tulos. O primeiro cap?tulo configura-se na introdu??o, onde se apresenta a base te?rica da disserta??o, pautada pelos conceitos de campo jornal?stico (Bourdieu), visualidades (Maneses), fotografia p?blica (Mauad), mem?ria (Candau) e sobreviv?ncia (Didi-Huberman). No segundo cap?tulo, realizou-se uma imers?o no contexto de constru??o da fotografia produzida no Brasil entre as d?cadas de 1960 e 1990. Destacaram-se aspectos do percurso profissional de fot?grafos cuja produ??o de imagens se vincula ? quest?o da terra. Tra?ou-se esse percurso para os fot?grafos e fot?grafas da s?rie do SINDJORS. Em seguida, apresentaram-se aspectos do percurso hist?rico dos movimentos sociais rurais no Rio Grande do Sul (Harres), (Eckert). Analisou-se como a constru??o das pr?ticas fotogr?ficas (Mauad) desses profissionais, dedicados a produ??o de imagens sobre a quest?o da terra, relaciona-se, em alguns aspectos, com a pr?pria constru??o da luta pela terra. Nos cap?tulos tr?s e quatro, atentando-se para as press?es do mercado (capital econ?mico) e pol?tico sobre o campo jornal?stico, identificaram-se algumas estrat?gias adotadas pelos ve?culos de comunica??o comercial para noticiar o acontecimento, que podem ser resumidas nestes pontos: criminalizar o ato do MST; desqualificar a a??o pol?tica, direcionando a pauta para a morte do soldado Valdeci de Abreu, descontextualizando a hist?ria da luta pela terra; e produzir as narrativas a partir da ideia de ?confronto?. Por fim, observou-se que a ideia de ?confronto? se fez presente na rela??o entre as fotografias e os textos publicados nos peri?dicos. As fotografias publicadas nos jornais analisados reclamam uma mem?ria de den?ncia da viol?ncia policial, contrapondo-se ao texto, que tende a valorizar a a??o dos policiais militares. No cap?tulo cinco, analisou-se a s?rie de imagens do SINDJORS, identificando seus padr?es e sobreviv?ncias, bem como as edi??es dos jornais sindicais (SINJORS e Sindibanc?rios) e do jornal ?Sem Terra? (MST). Constatou-se que a elabora??o discursiva do jornal ?Sem Terra? apresenta semelhan?as com as vers?es noticiadas pelo Jornal do SINDJORS e O Banc?rio. Por?m, em ?Sem Terra?, percebeu-se uma acentua??o maior na preocupa??o em inserir o acontecimento da Pra?a da Matriz sob o prisma do percurso hist?rico da luta pela terra no Brasil, destacando o cen?rio violento capitaneado pelos governos em rela??o ? quest?o da terra. Pode-se dizer que a constru??o das mem?rias visuais do acontecimento molda-se na disputa entre as vers?es do texto jornal?stico, condensadas na repeti??o e reprodu??o, e as visualidades das fotografias, calcadas na tens?o entre resist?ncia e repress?o e nas sobreviv?ncias de imagens de outras temporalidades.
69

Mem?rias em disputa: comemora??es do Dia do ?ndio nos jornais Correio da Bahia e Tribuna da Bahia (1990-2000)

Souza, Rafaela dos Santos 27 July 2016 (has links)
Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-10-07T23:55:20Z No. of bitstreams: 1 DISSERTA??O FINAL (2) RAFAELA DOS SANTOS SOUZA.pdf: 1576436 bytes, checksum: ff7923de624d7a26178be6a96b2d6495 (MD5) / Made available in DSpace on 2016-10-07T23:55:20Z (GMT). No. of bitstreams: 1 DISSERTA??O FINAL (2) RAFAELA DOS SANTOS SOUZA.pdf: 1576436 bytes, checksum: ff7923de624d7a26178be6a96b2d6495 (MD5) Previous issue date: 2016-07-27 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / This paper analyzes the discourses and images of indigenous peoples conveyed by the newspaper Correio da Bahia and Tribuna da Bahia from Indian Day celebrations during the decade of 1990-2000. This decade was marked by debates on national and international levels, providing greater visibility to indigenous issues. We propose a reflection on the images broadcast from the celebrations of April 19, aiming to question the production of discourses on these subjects in the construction and maintenance of memories about the indigenous peoples, reflecting on the social role of the celebrations. For its political character, the celebrations are here understood as construction of social memory. Think the holidays as creating memory of these people makes it possible to reflect on the role of Bahia written press has played with regard to indigenous peoples. We also analyzed the possible dialogues and influences between the discursive productions evidenced through the analysis of materials of the newspapers Correio da Bahia and Tribuna da Bahia Bahia and speeches produced by authors in Brazilian history during the nineteenth and twentieth centuries in the construction of images about the Indians who contribute the formation and perpetuation of memories about these subjects. / Esse trabalho analisa os discursos e as imagens do povo ind?gena, veiculados pelos jornais Correio da Bahia e Tribuna da Bahia a partir das comemora??es do Dia do ?ndio durante a d?cada de 1990-2000. Essa d?cada foi marcada por debates no ?mbito nacional e internacional, proporcionando maior visibilidade ?s quest?es ind?genas. Propomos uma reflex?o sobre as imagens difundidas a partir das comemora??es do 19 de Abril, visando questionar a produ??o de discursos sobre esses sujeitos na constru??o e manuten??o de mem?rias acerca dos povos ind?genas, refletindo sobre o papel social das comemora??es. Por seu car?ter pol?tico, as comemora??es s?o aqui compreendidas enquanto construtoras de mem?ria social. Pensar as datas comemorativas como criadoras de mem?ria sobre esse povo possibilita refletir sobre o papel que a imprensa escrita da Bahia vem desempenhando no que diz respeito aos povos ind?genas. Analisamos tamb?m os poss?veis di?logos e influ?ncias entre as produ??es discursivas evidenciadas atrav?s das an?lises das mat?rias dos jornais Correio da Bahia e Tribuna da Bahia e os discursos produzidos por autores da historiografia brasileira durante os s?culos XIX e XX na constru??o das imagens acerca do ind?gena, que contribuem para a forma??o e a perpetua??o de mem?rias sobre esses sujeitos.
70

Literatura e cultura: narrar a identidade religiosa em Viva o povo brasileiro de Jo?o Ubaldo Ribeiro

Silva, Ros?ngela Santos 06 April 2015 (has links)
Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-07-12T22:56:37Z No. of bitstreams: 1 PROGEL_DISSERT_2015_ROSANGELA (1) (1).pdf: 3601970 bytes, checksum: aa02813bdb188a025deb27e3303a36e4 (MD5) / Made available in DSpace on 2017-07-12T22:56:37Z (GMT). No. of bitstreams: 1 PROGEL_DISSERT_2015_ROSANGELA (1) (1).pdf: 3601970 bytes, checksum: aa02813bdb188a025deb27e3303a36e4 (MD5) Previous issue date: 2015-04-06 / This thesis sought to understand and analyze the narratives and representations regarding the ritualistic cult egwugwu nanny in Itaparica / BA Island from the literary Long live the Brazilian people (1984) of itaparicano Jo?o Ubaldo Ribeiro. Assuming that the theme of religiosity, this work is related to identity projects based on memory and oral aimed to reflect on the forms of African descent resistance at keeping alive the religious basis of their ancestors and how the feeling of belonging a particular religion becomes a key factor in the construction of cultural identity. The study is based on the reflection of dialogical relations between memory and orality, ritual egwugwu and performance practices carried out in Afro-Brazilian religions. Categories such as ancestry, oral, performance and ritual were triggered as reading operators, in line with the theoretical, critical and historical postulates of Literature scholars of African and Afro-Brazilian cultures. In the particular case of this work, one of the many outstanding contributions is by proposing a model of understanding and analysis of the dynamics of public performances of Candombl?. Thus, the research allowed to present a new look about the religions of African origin and their practices showing how Jo?o Ubaldo Ribeiro to transcribe these facts, it highlights something essential that is respect and appreciation for these religions who still suffer prejudice and discrimination. / A presente disserta??o procurou compreender e analisar as narrativas e representa??es a respeito do culto ritual?stico de bab? egwugwu na Ilha de Itaparica/BA a partir da obra liter?ria Viva o povo brasileiro (1984) do itaparicano Jo?o Ubaldo Ribeiro. Partindo do pressuposto de que a tem?tica da religiosidade, nessa obra, est? relacionada com projetos identit?rios baseados na mem?ria e oralidade objetivou-se refletir sobre as formas de resist?ncia do afrodescendente em manter vivas as bases religiosas de seus antepassados e como o sentimento de pertencimento a determinada religi?o torna-se fator fundamental na constru??o da identidade cultural. O estudo se assenta na reflex?o das rela??es dial?gicas entre mem?ria e oralidade, ritual?stica egwugwu e pr?ticas perform?ticas realizadas nas religi?es afrobrasileiras. Categorias como: ancestralidade, oralidade, performance e ritual?stica foram acionadas como operadores de leitura, em conson?ncia com os postulados te?ricos, cr?ticos e hist?ricos de estudiosos da Literatura, das culturas africanas e afrobrasileiras. No caso particular desse trabalho, uma das in?meras contribui??es relevantes se d? pela proposta de um modelo de entendimento e an?lise da din?mica das performances p?blicas do Candombl?. Assim, a pesquisa permitiu apresentar um novo olhar acerca das religi?es de matriz africana e suas pr?ticas mostrando como Jo?o Ubaldo Ribeiro, ao transcrever tais fatos, coloca em evid?ncia algo essencial que ? o respeito e valoriza??o por tais religi?es que ainda sofrem preconceito e descrimina??o.

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