No description available.

This study examines a possible relationship between Total Body Water (TBW) levels, osteoarthritic pain and functional performance in a sample of untrained adults. Participants complete a Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire, TBW is measured using single-frequency bioelectrical impedance (SF-BIA) and strength, balance and physical function are measured by the completion of 7 standardized functional tests. Participants in this study will include adult men and women, age 35 years and older, who have been previously diagnosed with Osteoarthritis (OA) in the hip or knee, or who score 35 or higher on the WOMAC. This study aims to determine if TBW values, specifically its intracellular compartment (ICW), will have a relationship to WOMAC scores and if intracellular water (ICW) will have a positive correlation to participants’ overall performance on balance, strength, and physical function tests. It is hypothesized that the data will show a negative correlation between ICW and WOMAC scores and a positive correlation between ICW and performance on balance, strength and physical function tests. Little research exists on the relationship between TBW and functional performance in older adults; particularly those whose TBW may be affected by inflammatory conditions such as OA. A goal of this study is to contribute to existing research on the relationship between performance and TBW, while providing insight and data on this relationship in an untrained population.

No description available.

Type II collagen, which gives the cartilage its tensile strength, is destroyed in osteoarthritis (OA). Cathepsin K is recognized as capable of cleaving type II collagen, however, the regulation of its activity in the cartilage is little known. Our hypothesis is that the activity of cathepsin K in cartilage is measured by an ELISA specific to cathepsin K cleavage site. A new specific ELISA (C2K77) was developed and tested by measuring the activity of the exogenous cathepsin K. The ELISA C2K77 was then used to measure the activity of the endogenous cathepsin K in equine articular cartilage explants cultured with or without stimulation (IL-1β, TNF-α, Oncostatin M (OSM) and LPS). Then the activity of cathepsin K was compared to that of MMPs (C1,2C ELISA) in the cartilage explants and in the freshly harvested cartilage. A significant difference was observed between normal cartilage and cartilage digested with cathepsin K (p˂0.01). There was no significant difference in the content of C2K77 between the control group and the groups stimulated while the content of C1,2C was increased by the combination of IL-1β and OSM (p = 0.002) and TNF-α and OSM (p˂0.0001). The new ELISA C2K77 demonstrates the ability to measure the activity of cathepsin K and revealed that there is a difference between the regulation of cathepsin K and MMP in articular cartilage. / Le collagène de type II, qui confère au cartilage articulaire sa résistance à la tension, est détruit dans l’arthrose. La cathepsine K est reconnue comme pouvant cliver le collagène de type II. Cependant, la régulation de son activité dans le cartilage est peu connue. Notre hypothèse est que l’activité de la cathepsine K dans le cartilage est mesurable par une ELISA spécifique au site de clivage de la cathepsine K. Une nouvelle ELISA spécifique (C2K77) a été développée et testée en mesurant l’activité de la cathepsine K exogène. L’ELISA C2K77 a ensuite été utilisée pour mesurer l’activité de la cathepsine K endogène dans des explants de cartilage articulaire équin mis en culture avec ou sans stimulations (IL-1β, TNF-α, oncostatine M (OSM) et le LPS). Puis l’activité de la cathepsine K a été comparée à celle des MMPs (avec l’ELISA C1,2C) dans les explants de cartilage et dans le cartilage fraichement récolté. Une différence significative a été observée entre le cartilage normal et le cartilage digéré avec la cathepsine K exogène (p˂0.01). Il n’y avait aucune différence significative dans la quantité de C2K77 entre le groupe control et les groupes stimulés tandis que la quantité de C1,2C a été augmenté par la combinaison de l’IL-1β et de l’OSM (p=0.002) et du TNF-α et de l’OSM (p˂0.0001). La nouvelle ELISA C2K77 démontre la capacité de mesurer l’activité de la cathepsine K et a permis de voir qu’il y a une différence entre la régulation de la cathepsine K et des MMPs.

Motivation is low for publishing and self-archiving in institutional repositories according to previous research, which is an issue since the repository’s full potential cannot be reached if it is void of materials. Doctoral students are the future of academia and ought to be given tools and information to be able to make informed decisions about where and how to publish. The purpose of this study is to shed light on possibilities and problems regarding self-archiving in institutional repositories by examining doctoral students at the University of Borås, focussing on their opinions of and experiences with the institutional repository DiVA as well as their perceived risks and benefits with self-archiving. Qualitative data were generated through five semi-structured interviews over videocalls. Respondents were doctoral students at the University of Borås with different levels of experience with publishing and self-archiving. The data was coded thematically and analysed using qualitative content analysis. Theories of constructivism and information literacy were applied to discuss the results. The respondents were generally knowledgeable concerning different levels of Open Access but seemed unsure of the purpose and function of DiVA. All self-archived because of the University’s policy, however not everyone recognized the personal benefits. This study concludes that research practices can be identified and problematized by acknowledging disciplinary differences in scholarly communication. Doctoral students are in a unique position to take the lead in forming research traditions that fit the new scientific landscape.

No description available.

No description available.

Einleitung: Degenerativ-entzündliche Gelenkerkrankungen und insbesondere die Osteoarthritis (OA) gehören zu den häufigsten orthopädischen Erkrankungen beim Pferd und führen in vielen Fällen zu langfristigen und teilweise dauerhaften Leistungseinschränkungen. Ätiologisch ist die OA bei jungen Pferden hauptsächlich auf ein akutes oder repetitives Trauma zurückzuführen, während bei älteren Pferden von einem chronisch-degenerativen Krankheitsprozess ausgegangen werden kann. Zahlreiche klinische Studien konnten vielversprechende Therapieerfolge mit der intraartikulären Anwendung von potenziell regenerativ wirkenden Therapeutika aus autologen und allogenen Blutprodukten oder mesenchymalen Stromazellen (MSC) in unterschiedlichen OA-Modellen darlegen. Allerdings besteht aufgrund der heterogenen Studienlage bisher weitgehend Unklarheit über den Langzeiterfolg dieser sogenannten Orthobiologika bei Pferden mit natürlich auftretender OA. Zielsetzung: Die Zielsetzung dieser Arbeit war es, über eine statistische Aufarbeitung der Studienlage aus den Jahren 2007-2020 eine fundierte Aussage zu Langzeiterfolgen und zur Anwendungssicherheit von intraartikulär applizierten Orthobiologika bei natürlich auftretender OA beim Pferd zu treffen. Es wurde die Hypothese aufgestellt, dass die Anwendung von Orthobiologika eine sichere und effektive Therapieoption bei Pferden mit natürlich auftretender OA ist. Methoden: Für die Beurteilung wurde ein systematisches Review der aktuell publizierten Literatur in englischer und deutscher Sprache der Jahre 2007 bis 2020 mit anschließender Meta-Analyse angefertigt und ausgewertet. Die Literaturrecherche erfolgte über öffentlich zugängliche, anerkannte Wissenschaftsplattformen unter Nutzung definierter Schlüsselwörter. Es wurden zunächst alle klinischen Studien, die sich mit der Anwendung von Orthobiologika bei natürlich entstandener OA beim Pferd auseinandersetzen gesammelt und entsprechend festgelegter Ein- und Ausschlusskriterien selektiert. Für das systematische Review wurden klinische Studien, die eine intraartikuläre Behandlung mit MSC und/oder autologen Blutprodukten mit einer sechsmonatigen oder längeren Nachuntersuchungszeit der Pferde erfasst. Verglichen wurde der Lahmheitsgrad, gemessen an den Erfolgen in Wettkämpfen, Rückkehr in den Turniersport oder der Nutzung auf dem ursprünglichen Leistungsniveau, vor und nach der intraartikulären Behandlung. Zudem wurden auftretende Nebenwirkungen nach der Behandlung dokumentiert. Für die Meta-Analyse wurden ausschließlich randomisierte und kontrollierte Studien (RCTs) genutzt. Exkludiert wurden in-vivo-Studien mit induzierter OA, invitro- Studien oder Studien mit konventionellen Behandlungsmethoden. Jede Studie des systematischen Reviews wurde auf systematische Fehler untersucht und auf das Biasrisiko gemäß den „Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA)“-Leitlinien getestet. Um die Ergebnisse mittels der Methodik der Meta-Analyse zu vergleichen, wurde das Quotenverhältnis (Odds Ratio) mit einem 95 % Konfidenzintervall berechnet. In einem random-effects Modell wurde jede Studie mit ihrer geschätzten Effektgröße und dem entsprechenden Konfidenzintervall im Forest Plot grafisch dargestellt. Die Heterogenität wurde mit I² für die Studien berechnet und mit einem Ergebnis von I² > 50 % als signifikant eingestuft. Ein Ergebnis wurde als signifikant angesehen, wenn sich p < 0,05 darstellte. Ergebnisse: Die elektronische und manuelle Recherche ergab insgesamt 271 Studien. Davon erfüllten 13 Studien die Einschlusskriterien für das systematische Review, wobei in fünf Studien die OA mit MSC behandeltet wurde. In acht der 13 Studien wurden autologe Blutprodukte verabreicht, davon wurde in drei Studien autologes konditioniertes Serum (ACS) angewandt. In den verbleibenden fünf Studien wurden zelluläre Eigenblutprodukte mit einer erhöhten Thrombozytenkonzentration verwendet. Insgesamt wurden bei zwei der 13 Studien ein geringes Risiko für eine statistische Verzerrung festgestellt. Nach der intraartikulären Behandlung mit orthobiologischen Therapeutika wurde eine durchschnittliche Verbesserung des Lahmheitsgrades um 65 % erreicht, unabhängig von der Art des verwendeten Orthobiologikums. Elf der 13 Studien zeigten einen generellen positiven Effekt nach der Behandlung, mit Pferden die auf ihr ursprüngliches Leistungsniveau zurückkehrten. Vier der 13 Studien erwiesen sich geeignet für die Meta-Analyse und wurden mittels Forest Plot exprimiert. Hier wurde die Reduktion des Lahmheitsgrades der Behandlungs- und der Kontrollgruppe verglichen. Die Heterogenität der Langzeitstudien mit einem Beobachtungszeitraum von mindestens sechs Monaten war moderat mit einem Heterogenitätsindex von I² = 55 %. Alle Studien mit einem OR-Wert > 1 begünstigen die experimentelle Gruppe, was hier zutreffend war (OR 17,02; 95 % CI: 8,5474 bis 33,8849 p < 0,0001). In keiner Studie wurde die Grenze zur Unwirksamkeit überschritten, was darauf hindeutet, dass der Behandlungseffekt in allen Studien als ähnlich eingeschätzt wurde. In der Langzeitbeobachtung führte die Anwendung von intraartikulär verabreichten orthobiologischen Therapeutika zu einer Verringerung der Lahmheit bei 73 %, während die Lahmheit in der Kontrollgruppe bei 17 % reduziert wurde. Transiente Nebenwirkungen im Sinne eines sogenannten „joint flare“, einer aseptischen, entzündlichen Gelenkreaktion, waren in allen dokumentierten Fällen (n = 15, 3 %) der insgesamt 518 ausgewerteten Pferde überwiegend selbstlimitierend und führten in nur einem Fall aufgrund fehlender Aufrechterhaltung der Verblindung zu einem Studienausschluss. Schlussfolgerung: Den eingeschlossenen Studien zufolge zeigten Pferde mit natürlich auftretender OA nach einer intraartikulären Behandlung mit orthobiologischen Therapeutika im Vergleich zur Kontrollgruppe im Langzeit-Follow-up einen signifikant geringeren Grad an Lahmheit bei hoher Anwendungssicherheit. Keines der Pferde zeigte nach Behandlung signifikante Nebenwirkungen.:Inhaltsverzeichnis Abbildungsverzeichnis Abkürzungsverzeichnis Tabellenverzeichnis 1. Einleitung 2. Literaturübersicht 2.1 Die Osteoarthritis beim Pferd 2.1.1 Ätiologie beim Pferd 2.1.2 Pathomechanismus der Osteoarthritis 2.1.3 Osteoarthritis auf zellulärer Ebene 2.1.4 Histologische Studien zur Osteoarthritis beim Pferd 2.2 Regenerative/-Orthobiologische Therapien bei Osteoarthritis 2.2.1 Das therapeutische Konzept orthobiologischer Therapieansätze 2.2.2 Behandlungsoptionen der Osteoarthritis beim Pferd 2.2.3 Osteoarthritis: Das Pferd als Modelltier 2.3 Evidenzbasierte Medizin 2.3.1 Definition der evidenzbasierten Medizin 2.3.2 Das Systematische Review 2.3.3 Risk of bias 2.3.4 Die Meta-Analyse 3. Publikation 4. Diskussion 4.1 Methodische Diskussion der Studienauswertung 4.2 Kritische Begutachtung verschiedener OA-Modelle 4.3 Heterogenität der Studien 4.3.1 Diversität der Behandlungsmethoden 4.3.2 Uneinheitliche Definition des Erfolgsmerkmals und der Untersuchung 4.4 Kritik an randomisierten, kontrollierten Studien 4.5 Kritik an der Meta-Analyse 4.6 Schlussfolgerung 5. Zusammenfassung 6. Summary Literaturverzeichnis Danksagung / Introduction: Degenerative-inflammatory joint diseases, specifically osteoarthritis (OA), are among the most common orthopedic diseases in horses and usually lead to long-term and even permanent performance limitations. Etiologically, OA in young horses is mainly caused by an acute or repetitive joint trauma, whereas chronic degenerative process is stated in older horses. Numerous clinical studies have been demonstrated promising therapeutic success by intra-articular application of potentially regenerative therapeutics derived from autologous and allogeneic blood products or mesenchymal stromal cells (MSCs) in different OA models. However, the long-term success of these so named orthobiologics in horses with naturally occurring OA is controversially discussed due to the heterogeneity of studies. Objectives: The objective of this study was a statistical analysis of published literature between the years 2007-2020 regarding long-term success and safety of intraarticular orthobiologics in naturally occurring OA in horses. It was hypothesized that the use of orthobiologics is a safe and effective therapeutic option in horses with naturally occurring OA. Material and Methods: For assessment, a systematic review of the currently published literature in English and German from 2007-2020 with subsequent meta-analysis was performed and evaluated. The literature search was conducted via publicly available, recognized scientific platforms using defined keywords. First, all clinical studies dealing with the use of orthobiologics in naturally occurring OA in horses were reviewed and selected according to defined inclusion and exclusion criteria. All clinical trials that included intraarticular treatment with MSCs and/or autologous blood products with an at least six-month follow-up were included in the systematic review. The degree of lameness prior and after intraarticular treatment was evaluated (comparison of the success rate, horses working on competition, horses working at trainings level, lame free horses). In addition, any adverse effects that occurred after treatment were documented. For meta-analysis only randomized controlled trials (RCTs) were included. In vivo studies with chemically or experimentally induced OA, in vitro studies or studies used conventional treatments were excluded. Each study included in the systematic review was examined to the risk of bias according to the „preferred reporting items for systematic reviews and meta-analyses (PRISMA)” guidelines. To compare dichotomous outcomes via meta-analysis, an odds ratio (OR) with 95 % confidence interval (CI) was calculated. A random-effects model was used describing the overall outcome in a forest plot. The I² statistic was used to assess heterogeneity with a result of I² > 50 % classified as significant. Overall, a result was considered significant with p < 0,05. Results: The initial electronic and manual research resulted in a total of 271 studies. Of these, 13 studies met the inclusion criteria for the systematic review, whereof five studies used MSCs as an orthobiologic treatment option. In three of the 13 studies administered autologous conditioned serum (ACS) and the remaining five studies used cellular autologous blood products with an increased platelet concentrate. Overall, two studies considered to be at a low risk of bias. After intra-articular treatment with orthobiologic therapeutics, an average improvement of 65 % in lameness was achieved, regardless the type of orthobiologic agent used. Eleven of the 13 studies showed a general positive effect after treatment with horses returning to their original performance level. Four of the 13 studies proved suitable for metaanalysis and were expressed using forest plot. The reduction in the degree of lameness between the treatment and control groups was compared. There was moderate heterogeneity in the long-term studies with an observation period of at least six months, I² = 55 %. All studies with an OR value > 1 favor the experimental group, which was appropriate here (OR 17,02; 95 % CI: 8,5474 to 33,8849 p < 0,0001). No study crossed the line into ineffectiveness, indicating that the treatment effect was considered similar among the studies. In the long-term follow-up, the use of intraarticularly administered orthobiologics led to a reduction in lameness in 73 % reduction in lameness, while lameness was reduced in 17 % in the control group. All documented cases (n = 15, 3 %) of the total 518 horses evaluated experienced transient adverse effects, also referred to as “joint flare”, an aseptic, inflammatory, and in most cases self-limiting joint reaction. One case resulted in exclusion from the study because blinding could not be maintained. Conclusion: According to the included studies, horses with naturally occurring OA after intraarticular treatment with orthobiologic therapeutics demonstrated a significantly lower degree of lameness at long-term follow-up compared to the control group, with a high level of safety of use. None of the horses showed significant side effects after treatment.:Inhaltsverzeichnis Abbildungsverzeichnis Abkürzungsverzeichnis Tabellenverzeichnis 1. Einleitung 2. Literaturübersicht 2.1 Die Osteoarthritis beim Pferd 2.1.1 Ätiologie beim Pferd 2.1.2 Pathomechanismus der Osteoarthritis 2.1.3 Osteoarthritis auf zellulärer Ebene 2.1.4 Histologische Studien zur Osteoarthritis beim Pferd 2.2 Regenerative/-Orthobiologische Therapien bei Osteoarthritis 2.2.1 Das therapeutische Konzept orthobiologischer Therapieansätze 2.2.2 Behandlungsoptionen der Osteoarthritis beim Pferd 2.2.3 Osteoarthritis: Das Pferd als Modelltier 2.3 Evidenzbasierte Medizin 2.3.1 Definition der evidenzbasierten Medizin 2.3.2 Das Systematische Review 2.3.3 Risk of bias 2.3.4 Die Meta-Analyse 3. Publikation 4. Diskussion 4.1 Methodische Diskussion der Studienauswertung 4.2 Kritische Begutachtung verschiedener OA-Modelle 4.3 Heterogenität der Studien 4.3.1 Diversität der Behandlungsmethoden 4.3.2 Uneinheitliche Definition des Erfolgsmerkmals und der Untersuchung 4.4 Kritik an randomisierten, kontrollierten Studien 4.5 Kritik an der Meta-Analyse 4.6 Schlussfolgerung 5. Zusammenfassung 6. Summary Literaturverzeichnis Danksagung

A artrose, também chamada de osteoartrose ou osteoartrite (OA), é a terceira doença de maior incidência no Mundo. Nesse trabalho, buscou-se criar uma lesão osteocartilaginosa em joelhos de ratos Wistar machos com seis meses de vida, objetivando constituir um modelo animal para estudo da OA humana e, a partir desse modelo, avaliar biomateriais de forma isolada ou associados entre si e avaliados quanto à sua segurança biológica e potencial de reparação tecidual. Além disto, foi analisado o efeito reparador de células-tronco mesenquimais da polpa do dente de leite humano (MSC) isoladamente e em associação com o biomaterial formado por: Titânio + Poli Vinil Álcool + Ac. Hialurônico, nesse estudo denominado de TRIGEL (TRG). O Ac. Hialurônico (HA), por suas propriedades visco elásticas, o pó de Titânio (Ti), devido às suas propriedades biológicas únicas de ostoeintegração e o polímero Poli Vinil Álcool (PVA), com suas propriedades hidrofílicas, promovendo a formação do Hidrogel, os quais associados entre si formam um compósito, o TRG, que foi aplicado sobre uma lesão padrão no joelho da pata pélvica direita de ratos Wistar machos de seis meses de idade. Para a obtenção da lesão padrão, os animais foram divididos em três grupos de cincoanimais e cada grupo foi submetido a uma intervenção cirúrgica em seus joelhos direitos, utilizando três técnicas cirúrgicas diferentes, a saber: Grupo (I): Remoção cirúrgica dos meniscos medial e lateral mais perfuração do platô tibial seguido da aspiração da medula óssea através dessa perfuração por meio de seringa e agulha. Grupo (II): Remoção dos meniscos mais perfuração, sem aspiração, Grupo (III): Apenas a perfuração. Todos os animais foram autopsiados após 30 dias. Os joelhos dos quinze animais que constituíam os três grupos foram devidamente catalogados e enviados para a empresa Histotech, para a confecção das lâminas, tendo sido eleito, por análise histológica, o Grupo (I), por demonstrar menor reparo tecidual espontâneo. Em tese, o TRG teria as seguintes propriedades: Uma fonte de reparação tecidual (visco terapia) dada pelo HA e a capacidade amortecedora e carreadora de células-tronco do polímero PVA, que se hidrata, formando o hidrogel. O Ti, pela sua propriedade de osteointegração formaria um tampão sobre as áreas de matriz óssea exposta o que possibilitaria o afluxo de novos condrócitos, que também pode ocorrer pela ação das células-tronco. Livrar a superfície articular das áreas com exposição da matriz óssea é fundamental para o bloqueio das proteases que perpetuam a fisiopatologia da OA. Após tratamento estatístico dos diversos ensaios, utilizando-se os diversos biomateriais no tratamento da lesão, o TRG foi o biomaterial que apresentou o melhor resultado de força entre os grupos. No estudo histológico, foi evidenciada a presença de tecido cartilaginoso supra- lesional, o que só ocorreu nos animais dos grupos que receberam: apenas TRG, TRG associado com células-tronco e aquele que recebeu apenas MSCs. No entanto, mais estudos, com animais de maior porte e mais velhos, devem ser realizados para melhor analisar a segurança e o potencial terapêutico do compósito Trigel. / Osteoarthritis, or osteoarthritis (OA) is the third most debilitating disease in the world. In this study, we attempted to create an osteocartilaginous lesion in the knees of six months old male Wistar rats, aiming to constitute an animal model for the study of human OA and to use this model to evaluate the therapeutic potential of biomaterials, which are already well known for their biocompatibility properties in the clinical practice. The biomaterials were used in isolation or associated with each other and then evaluated for their biological safety and tissue repair capacity. Mesenchymal stem cells, obtained from human dental pulp from deciduous teeth (MSC) were evaluated alone and in association with the biomaterial formed by: Titanium + Poly Vinyl Alcohol + Ac. Hyaluronic, here called TRIGEL (TRG). Due to its visco-elastic properties, the Ti powder, due to its unique biological properties of ostointegration and the polymer PVA, with its hydrophylic properties, forming a hydrogel, were associated to form the composite named TRIGEL, (TRG), which was applied to a standard knee injury of the right hind leg of male Wistar rats. In order to elect the standard lesion, the animals were divided into three groups with five animals each and each group underwent a surgical intervention in their right knees, with three different surgical techniques being applied, namely: Group (1): Surgical removal ofmedial and lateral meniscus plus perforation of the tibial plateau, followed by aspiration of the bone marrow through this perforation using syringe and needle. Group (2): Removal of the meniscus plus perforation, without aspiration, Group (3): Drilling only. All groups were autopsied 30 days after the procedure and all groups were autopsied at 30 days post-procedure. The knees of the 15 animals that constituted the three groups were analyzed histologically and Group (1) (meniscus removed, perforated and aspirated), was elected as the standard lesion since it demonstrated less spontaneous tissue repair. TRG has the following properties: HA is used as a source of tissue repair (visco therapy) and hydration of the polymer; PVA, forms a hydrogel\", with damping action and as a stem cells carrier, whereas Ti was used due to its ósseo-integration, which would allow coating of the exposed bone matrix and this intra-osseous osteo-integration response would form an intercalating buffer. The healthy cartilage surfaces around this structural buffer would allow the reception of new chondrocytes or the action of the cells on TRG properties. Freeing the articular surface of the areas with bone matrix exposure is critical for blocking the proteases that perpetuate the pathophysiology of OA. In the various biomaterial tests in the treatment of the standard lesions, TRG was statistically shown to be the one that better mimicked the non-injured group. The histological study demonstrated the presence of a supra-lesional cartilagenous tissue, which only occurred in the groups which received: only TRG, mesenchymal stem cells associated with TRG and that which received only MSCs. However, further studies with larger and older animals should be pursued to better assess the safety and therapeutic potential of the Trigel composite.

A artrose, também chamada de osteoartrose ou osteoartrite (OA), é a terceira doença de maior incidência no Mundo. Nesse trabalho, buscou-se criar uma lesão osteocartilaginosa em joelhos de ratos Wistar machos com seis meses de vida, objetivando constituir um modelo animal para estudo da OA humana e, a partir desse modelo, avaliar biomateriais de forma isolada ou associados entre si e avaliados quanto à sua segurança biológica e potencial de reparação tecidual. Além disto, foi analisado o efeito reparador de células-tronco mesenquimais da polpa do dente de leite humano (MSC) isoladamente e em associação com o biomaterial formado por: Titânio + Poli Vinil Álcool + Ac. Hialurônico, nesse estudo denominado de TRIGEL (TRG). O Ac. Hialurônico (HA), por suas propriedades visco elásticas, o pó de Titânio (Ti), devido às suas propriedades biológicas únicas de ostoeintegração e o polímero Poli Vinil Álcool (PVA), com suas propriedades hidrofílicas, promovendo a formação do Hidrogel, os quais associados entre si formam um compósito, o TRG, que foi aplicado sobre uma lesão padrão no joelho da pata pélvica direita de ratos Wistar machos de seis meses de idade. Para a obtenção da lesão padrão, os animais foram divididos em três grupos de cincoanimais e cada grupo foi submetido a uma intervenção cirúrgica em seus joelhos direitos, utilizando três técnicas cirúrgicas diferentes, a saber: Grupo (I): Remoção cirúrgica dos meniscos medial e lateral mais perfuração do platô tibial seguido da aspiração da medula óssea através dessa perfuração por meio de seringa e agulha. Grupo (II): Remoção dos meniscos mais perfuração, sem aspiração, Grupo (III): Apenas a perfuração. Todos os animais foram autopsiados após 30 dias. Os joelhos dos quinze animais que constituíam os três grupos foram devidamente catalogados e enviados para a empresa Histotech, para a confecção das lâminas, tendo sido eleito, por análise histológica, o Grupo (I), por demonstrar menor reparo tecidual espontâneo. Em tese, o TRG teria as seguintes propriedades: Uma fonte de reparação tecidual (visco terapia) dada pelo HA e a capacidade amortecedora e carreadora de células-tronco do polímero PVA, que se hidrata, formando o hidrogel. O Ti, pela sua propriedade de osteointegração formaria um tampão sobre as áreas de matriz óssea exposta o que possibilitaria o afluxo de novos condrócitos, que também pode ocorrer pela ação das células-tronco. Livrar a superfície articular das áreas com exposição da matriz óssea é fundamental para o bloqueio das proteases que perpetuam a fisiopatologia da OA. Após tratamento estatístico dos diversos ensaios, utilizando-se os diversos biomateriais no tratamento da lesão, o TRG foi o biomaterial que apresentou o melhor resultado de força entre os grupos. No estudo histológico, foi evidenciada a presença de tecido cartilaginoso supra- lesional, o que só ocorreu nos animais dos grupos que receberam: apenas TRG, TRG associado com células-tronco e aquele que recebeu apenas MSCs. No entanto, mais estudos, com animais de maior porte e mais velhos, devem ser realizados para melhor analisar a segurança e o potencial terapêutico do compósito Trigel. / Osteoarthritis, or osteoarthritis (OA) is the third most debilitating disease in the world. In this study, we attempted to create an osteocartilaginous lesion in the knees of six months old male Wistar rats, aiming to constitute an animal model for the study of human OA and to use this model to evaluate the therapeutic potential of biomaterials, which are already well known for their biocompatibility properties in the clinical practice. The biomaterials were used in isolation or associated with each other and then evaluated for their biological safety and tissue repair capacity. Mesenchymal stem cells, obtained from human dental pulp from deciduous teeth (MSC) were evaluated alone and in association with the biomaterial formed by: Titanium + Poly Vinyl Alcohol + Ac. Hyaluronic, here called TRIGEL (TRG). Due to its visco-elastic properties, the Ti powder, due to its unique biological properties of ostointegration and the polymer PVA, with its hydrophylic properties, forming a hydrogel, were associated to form the composite named TRIGEL, (TRG), which was applied to a standard knee injury of the right hind leg of male Wistar rats. In order to elect the standard lesion, the animals were divided into three groups with five animals each and each group underwent a surgical intervention in their right knees, with three different surgical techniques being applied, namely: Group (1): Surgical removal ofmedial and lateral meniscus plus perforation of the tibial plateau, followed by aspiration of the bone marrow through this perforation using syringe and needle. Group (2): Removal of the meniscus plus perforation, without aspiration, Group (3): Drilling only. All groups were autopsied 30 days after the procedure and all groups were autopsied at 30 days post-procedure. The knees of the 15 animals that constituted the three groups were analyzed histologically and Group (1) (meniscus removed, perforated and aspirated), was elected as the standard lesion since it demonstrated less spontaneous tissue repair. TRG has the following properties: HA is used as a source of tissue repair (visco therapy) and hydration of the polymer; PVA, forms a hydrogel\", with damping action and as a stem cells carrier, whereas Ti was used due to its ósseo-integration, which would allow coating of the exposed bone matrix and this intra-osseous osteo-integration response would form an intercalating buffer. The healthy cartilage surfaces around this structural buffer would allow the reception of new chondrocytes or the action of the cells on TRG properties. Freeing the articular surface of the areas with bone matrix exposure is critical for blocking the proteases that perpetuate the pathophysiology of OA. In the various biomaterial tests in the treatment of the standard lesions, TRG was statistically shown to be the one that better mimicked the non-injured group. The histological study demonstrated the presence of a supra-lesional cartilagenous tissue, which only occurred in the groups which received: only TRG, mesenchymal stem cells associated with TRG and that which received only MSCs. However, further studies with larger and older animals should be pursued to better assess the safety and therapeutic potential of the Trigel composite.