Caracterização molecular e susceptibilidade antimicrobiana de linhagens de Listeria monocytogenes isoladas de alimentos no RS Mestranda: Fernanda De Nes Orientador: Jeverson Frazzon Listeria monocytogenes é o microrganismo causador de listeriose, uma doença infecciosa adquirida através do consumo de alimentos contaminados e que afeta principalmente pessoas com o sistema imunológico comprometido como gestantes e recém nascidos. É um microrganismo ambiental, podendo ser encontrado no solo, água e alimentos como vegetais, produtos cárneos, leites crus ou mal pasteurizados, queijos, entre outros. São bactérias invasivas e possuem facilidade para penetrar na célula hospedeira. O ataque a essas células é intermediado pelas internalinas, que são proteínas associadas a genes de virulência. As internalinas InlA e InlB, codificadas pelo gene inlAB, são proteínas associadas com a invasão e internalização da bactéria na célula hospedeira. Este trabalho teve o objetivo de caracterizar um total de 19 linhagens de Listeria monocytogenes, sorovares 1/2b, 1/2a, 4b, 4c, isoladas de produtos lácteos do Rio Grande do Sul. Para a caracterização molecular foram utilizadas as técnicas moleculares de Amplificação Randômica do DNA Polimórfico (RAPD) e Análise por Enzimas de Restrição (PCRREA), com a amplificação de um segmento de 2916 pb que incluem partes dos genes inlA e inlB, utilizando para isso duas enzimas de restrição AluI e EcoRI. As técnicas moleculares utilizadas foram ferramentas úteis para caracterizar as linhagens de Listeria monocytogenes isoladas de produtos lácteos do Rio Grande do Sul. Ainda, foi observado o perfil de resistência antimicrobiana desses microrganismos frente a vários antimicrobianos, das amostras analisadas todas foram susceptíveis aos antimicrobianos testados. / Listeria monocytogenes is a microorganism which causes listeriosis, an infection disease caused by consuming contaminated food and it mainly affects individuals with the immune system at risk like pregnant women and newborn infants. It is an environmental microorganism, being found in soil, water and food like vegetables, meat products, unpasteurized milk and cheese. They are invasive bacteria which have special facility to penetrate the host cell. The attack to those cells is carried out by internalins, which are proteins associated with virulence genes. The InlA and InlB internalins, codified by inlAB gene, are proteins associated with the invasion and internalization of the bacteria in the host cell. This work aimed at categorizing a total of 19 strains of Listeria monocytogenes, 1/2b, 1/2a, 4b and 4c serovars, isolated from dairy products from the State of Rio Grande do Sul. For the molecular categorization, it was used the molecular techniques of Polymorphic DNA Random Amplification (RAPD) and Restriction Enzymatic Analysis (PCR-REA), with the amplification of a segment of 2916 pb which includes part of inlA and inlB genes, using for that purpose two restriction enzymes, AluI and EcoRI. The molecular techniques applied were useful tools to categorize strains of L. monocytogenes isolated from dairy products of the State of Rio Grande do Sul. Furthermore, it was observed the antimicrobial resistant profile of those microorganisms in front of various antimicrobials. All of the analyzed samples were susceptible to tested antimicrobials.

Caracterização molecular e susceptibilidade antimicrobiana de linhagens de Listeria monocytogenes isoladas de alimentos no RS Mestranda: Fernanda De Nes Orientador: Jeverson Frazzon Listeria monocytogenes é o microrganismo causador de listeriose, uma doença infecciosa adquirida através do consumo de alimentos contaminados e que afeta principalmente pessoas com o sistema imunológico comprometido como gestantes e recém nascidos. É um microrganismo ambiental, podendo ser encontrado no solo, água e alimentos como vegetais, produtos cárneos, leites crus ou mal pasteurizados, queijos, entre outros. São bactérias invasivas e possuem facilidade para penetrar na célula hospedeira. O ataque a essas células é intermediado pelas internalinas, que são proteínas associadas a genes de virulência. As internalinas InlA e InlB, codificadas pelo gene inlAB, são proteínas associadas com a invasão e internalização da bactéria na célula hospedeira. Este trabalho teve o objetivo de caracterizar um total de 19 linhagens de Listeria monocytogenes, sorovares 1/2b, 1/2a, 4b, 4c, isoladas de produtos lácteos do Rio Grande do Sul. Para a caracterização molecular foram utilizadas as técnicas moleculares de Amplificação Randômica do DNA Polimórfico (RAPD) e Análise por Enzimas de Restrição (PCRREA), com a amplificação de um segmento de 2916 pb que incluem partes dos genes inlA e inlB, utilizando para isso duas enzimas de restrição AluI e EcoRI. As técnicas moleculares utilizadas foram ferramentas úteis para caracterizar as linhagens de Listeria monocytogenes isoladas de produtos lácteos do Rio Grande do Sul. Ainda, foi observado o perfil de resistência antimicrobiana desses microrganismos frente a vários antimicrobianos, das amostras analisadas todas foram susceptíveis aos antimicrobianos testados. / Listeria monocytogenes is a microorganism which causes listeriosis, an infection disease caused by consuming contaminated food and it mainly affects individuals with the immune system at risk like pregnant women and newborn infants. It is an environmental microorganism, being found in soil, water and food like vegetables, meat products, unpasteurized milk and cheese. They are invasive bacteria which have special facility to penetrate the host cell. The attack to those cells is carried out by internalins, which are proteins associated with virulence genes. The InlA and InlB internalins, codified by inlAB gene, are proteins associated with the invasion and internalization of the bacteria in the host cell. This work aimed at categorizing a total of 19 strains of Listeria monocytogenes, 1/2b, 1/2a, 4b and 4c serovars, isolated from dairy products from the State of Rio Grande do Sul. For the molecular categorization, it was used the molecular techniques of Polymorphic DNA Random Amplification (RAPD) and Restriction Enzymatic Analysis (PCR-REA), with the amplification of a segment of 2916 pb which includes part of inlA and inlB genes, using for that purpose two restriction enzymes, AluI and EcoRI. The molecular techniques applied were useful tools to categorize strains of L. monocytogenes isolated from dairy products of the State of Rio Grande do Sul. Furthermore, it was observed the antimicrobial resistant profile of those microorganisms in front of various antimicrobials. All of the analyzed samples were susceptible to tested antimicrobials.

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2016-10-06T11:57:21Z No. of bitstreams: 1 2007 - Rafael Silva Barboza.pdf: 2062893 bytes, checksum: c7d9166e3fe66028a7acdadb02fed002 (MD5) / Made available in DSpace on 2016-10-06T11:57:21Z (GMT). No. of bitstreams: 1 2007 - Rafael Silva Barboza.pdf: 2062893 bytes, checksum: c7d9166e3fe66028a7acdadb02fed002 (MD5) Previous issue date: 2007-06-27 / Instituto Terra de Prote??o Ambiental, ITPA, Brasil. / The air basin is a three-dimensional space that conduct and distribute the winds over the drainage basin. In Rio de Janeiro?s state, 48% of the territory is constituted by Atlantic slope, where were registered 16 air basins with specific environmental features, distributed in 20% of the area. Based on the conformation of morphometrics parameters the air basins were grouped, by Multivariate Analysis - Cluster, with Euclidean distance of 48%, significance level of 95% and correlation coefficient of 0.7132, in 5 groups with similar forms. To evaluate the hydrologic effects within the air basins, it was used as a study case, the air basin of Angra dos Reis, where the magnitude of the interception of clouds was measured in the escape zone from the topographic divide of the Pirai river basin (leeward), in different distance away, with and without natural barriers, during the winter and summer. It was used vertical intercept sampling area equivalent to 0.45% of the forests in the region. The variation of the removal of the topographic divide occurred until 500 m of altitude, this limit may be the end of the escape zone and the beginning of the air basin distribution zone. The effect of air basin represented additional of 44% of the volume of rain in topographic divide (point 1), 39% at 4.5 km (point 2) and 42% at 7.5 km (point 3) of the point 1, in concordant direction of the flow. The effect of the orography in the trajectory of the winds, varied in an irregular way in winter and in summer, maintaining differential of 23% and 77% in point A (obstruction of 85% of the cross section) and 32% and 68% in point B (obstruction of 80% of the cross section). In areas under the influence of air basins, the variables and environmental processes can be crucial to offer environmental services in drainage basins. / A Bacia A?rea ? um espa?o tridimensional que conduz e distribui os ventos sobre as bacias hidrogr?ficas. No Estado do Rio de Janeiro, 48% do territ?rio ? composto pela vertente Atl?ntica, onde foram registradas 16 bacias a?reas com caracter?sticas ambientais espec?ficas, distribu?das em 20% da ?rea. Baseado na conforma??o de par?metros morfom?tricos as bacias a?reas foram agrupadas, atrav?s de An?lise Multivariada ? Cluster, com dist?ncia Euclidiana de 48%, n?vel de signific?ncia de 95% e coeficiente de correla??o de 0,7132 em 5 grupos com formas similares. Para avaliar os efeitos hidrol?gicos dentro das bacias a?reas, utilizou-se como estudo de caso a bacia a?rea de Angra dos Reis, onde a magnitude da vari?vel intercepta??o das nuvens foi medida na zona de escape, a partir do interfl?vio da bacia hidrogr?fica do rio Pirai (sotavento), em dist?ncias de afastamento distintas, com e sem barreiras orogr?ficas, durante o inverno e ver?o. Utilizou-se ?rea amostral de intercepta??o vertical equivalente a 0,45% das florestas da regi?o. A varia??o do afastamento do interfl?vio ocorreu at? desn?vel de 500 m em altitude, podendo ser este limite o fim da zona de escape e in?cio da zona de distribui??o da bacia a?rea. O efeito da bacia a?rea representou adicional de 44% em volume de chuva no interfl?vio (ponto 1), 39% a 4,5 km (ponto 2) e 42% a 7,5 km (ponto 3) do ponto 1 em dire??o concordante ao sentido do fluxo. O efeito da orografia no corredor variou de forma irregular no inverno e no ver?o, mantendo diferencial de 23% e 77% no ponto A (obstru??o de 85% da se??o transversal) e 32% e 68% , no ponto B (obstru??o de 80% da se??o transversal). Em ?reas sob influ?ncia de bacias a?reas, as vari?veis e processos ambientais podem ser determinantes para ofertar servi?os ambientais nas bacias hidrogr?ficas.

Made available in DSpace on 2014-12-17T14:00:14Z (GMT). No. of bitstreams: 1 AlanCMJ_DISSERT.pdf: 4554076 bytes, checksum: 7e3c48ac05771d7a7bf8bc3d0ad22556 (MD5) Previous issue date: 2011-06-22 / I propose with this paper a reflection on the experiences contained in the creation of the body- in-art (FERRACINI, 2006a, b) that originated the show Rosmaninhos... This process was developed within the coletivo UZUME teatro from Jo?o Pessoa PB, through recreations and resignifications of the corporeity and physicality contained in the steps, loas, aboios, songs and choreography observed in the manners that Mestre Zequinha plays in his group of Cavalo Marinho (Sea Horse), resident in the city of Bayeux - PB, and starting from the appropriation of the text Hamlet of William Shakespeare. The body-in-art is understood in this work as a vectorial body that dilates its daily functionality, recognizing a potential learning area capable to generate creative escape lines that destabilize the "subject centered in an individuality and identity" (FOUCAULT apud FERRACINI, 2006b, p.14), being open to the differentiation of itself, indicating the possible existence of an itself-other and of the exchange-in-art space. This process of construction of the body-in-art based on Master Zequinha s ways of playing the Cavalo Marinho was methodically guided by the appropriation of the coletivo UZUME teatro of the stages of Observation, Codification and Theatricalization contained in the technique of corporal mimeses proposed by the LUME Teatro (Campinas - SP). That use resulted in two phases: Active Observation and Composition of the body-in-art. Through the repetition of these aesthetic matrixes of the Cavalo Marinho, the actors discovered actions that when, codified and organized, can configure their body-in-art, which created a vectorial exchange-in-art space to what was found in the Cavalo Marinho party. This search proposed the means of potentiating the actors' work when it comes to a preparation that allowed to dilate the scenic presence and stimulated the production of actions, which culminated in the mounting of the show Rosmaninhos... / Proponho nesta disserta??o refletir sobre as experi?ncias contidas na cria??o dos corpos-em-arte (FERRACINI, 2006a, b) que originaram o espet?culo rosmaninhos.... Este processo foi desenvolvido junto ao coletivo UZUME teatro de Jo?o Pessoa PB, por meio de recria??es e resignifica??es das corporeidades e fisicidades contidas nos passos, loas, aboios, cantos e evolu??es observados nos modos como Mestre Zequinha brinca em seu grupo de Cavalo Marinho residente na cidade de Bayeux PB, e a partir da apropria??o do texto Hamlet de William Shakespeare. O corpo-em-arte ? entendido neste trabalho como um corpo vetorial que dilata sua funcionalidade cotidiana, reconhecendo uma zona potencial de aprendizado capaz de gerar linhas de fuga criativas que desestabilizem o sujeito centrado em uma individualidade e identidade (FOUCAULT apud FERRACINI, 2006b, p.14), abrindo-o a diferencia??o de si mesmo, indicando a possibilidade de exist?ncia de um si-outro e do espa?o de troca-em-arte. Este processo de constru??o do corpo-em-arte a partir das formas de Mestre Zequinha brincar o Cavalo Marinho, foi orientado metodologicamente pela apropria??o do coletivo UZUME teatro das etapas de Observa??o, Codifica??o e Teatraliza??o contidas na t?cnica de mimeses corp?rea proposta pelo LUME Teatro (Campinas SP). Esse uso resultou em duas fases: Observa??o Ativa e Composi??o do corpo-em-arte. Atrav?s da repeti??o destas matrizes est?ticas do Cavalo Marinho, os atores descobriram a??es que codificadas e organizadas configuram seus corpos-em-arte, os quais, por sua vez, deram origem a um espa?o de troca-em-arte vetorial ao encontrado no folguedo do Cavalo Marinho. Esta procura prop?s os meios de potencializar o trabalho dos atores no que diz respeito a uma prepara??o que permitisse dilatar a presen?a c?nica e estimulasse a produ??o de a??es, as quais culminaram na montagem do espet?culo rosmaninhos

Made available in DSpace on 2014-12-17T14:00:18Z (GMT). No. of bitstreams: 1 CarlaPM_DISSERT.pdf: 3215570 bytes, checksum: eba1e4312339fc642bcdc7da3ddb36ec (MD5) Previous issue date: 2013-03-25 / The present dissertation has begun with an investigation about the application of the Technique of the Corporeal Mimesis, systematized by the Lume, Interdisciplinary Center for Theatrical Research of Unicamp, associated with the matrix of Rural Maracatu, specifically of the group Cambinda Brasileira (Nazar? da Mata PE), with the purpose of promote the training and the composition work of the actor. Starting from an empirical methodology, which used the analysis of practical experiences the application process of the Corporeal Mimesis and the construction of a theater play associated with bibliographical research, I could investigate possible ways of re-signification of this technique, using components related to the ritual and the game present in the popular event in question, and experience such paths through the conception of the play Cravo do Canavial / Essa disserta??o se deu a partir de uma investiga??o sobre a aplica??o da T?cnica da M?mesis Corp?rea, sistematizada pelo Lume, N?cleo Interdisciplinar de Pesquisas Teatrais da Unicamp, associada ? matriz do Maracatu Rural, especificamente do grupo Cambinda Brasileira (Nazar? da Mata PE), a fim de fomentar o treinamento e o trabalho de composi??o do ator. A partir de uma metodologia emp?rica, que se utilizou da an?lise de experi?ncias pr?ticas processo de aplica??o da Mimese Corp?rea e montagem de espet?culo teatral associada ? pesquisa bibliogr?fica, pude investigar caminhos de poss?vel resignifica??o desta T?cnica, utilizando elementos referentes ao ritual e ao jogo presentes na manifesta??o popular em quest?o e experimentar atrav?s da concep??o do espet?culo Cravo do Canavial tais caminhos

Made available in DSpace on 2014-12-17T14:13:35Z (GMT). No. of bitstreams: 1 JoseWQ_TESE.pdf: 1778874 bytes, checksum: 7ff2c9f470b5b3873803d39a321ee362 (MD5) Previous issue date: 2009-10-05 / This work demonstrates the importance of using tools used in geographic information systems (GIS) and spatial data analysis (SDA) for the study of infectious diseases. Analysis methods were used to describe more fully the spatial distribution of a particular disease by incorporating the geographical element in the analysis. In Chapter 1, we report the historical evolution of these techniques in the field of human health and use Hansen s disease (leprosy) in Rio Grande do Norte as an example. In Chapter 2, we introduced a few basic theoretical concepts on the methodology and classified the types of spatial data commonly treated. Chapters 3 and 4 defined and demonstrated the use of the two most important techniques for analysis of health data, which are data point processes and data area. We modelled the case distribution of Hansen s disease in the city of Mossor? - RN. In the analysis, we used R scripts and made available routines and analitical procedures developed by the author. This approach can be easily used by researchers in several areas. As practical results, major risk areas in Mossor? leprosy were detected, and its association with the socioeconomic profile of the population at risk was found. Moreover, it is clearly shown that his approach could be of great help to be used continuously in data analysis and processing, allowing the development of new strategies to work might increase the use of such techniques in data analysis in health care / O presente trabalho demonstra a import?ncia do uso de ferramentas relacionadas ? sistemas de informa??o geogr?fica (SIG) e an?lise espacial de dados (AED) em estudos de doen?as infecciosas. M?todos de an?lise foram usados para descrever de forma mais abrangente a distribui??o espacial de doen?as a partir da incorpora??o do elemento geogr?fico na an?lise. No Cap?tulo 1 relata-se a evolu??o hist?rica da aplica??o destas t?cnicas no campo da sa?de humana e elege-se a hansen?ase no estado do Rio Grande do Norte como exemplo de aplica??o. No Cap?tulo 2 introduzem-se alguns conceitos te?ricos b?sicos sobre a metodologia e classificam-se os tipos de dados espaciais comumente tratados. Os Cap?tulos 3 e 4 definem e demonstram a utiliza??o das mais importantes t?cnicas de an?lise para os dois principais tipos de dados encontrados na sa?de, quais sejam dados de processos pontuais e dados de ?rea, focalizando como estudo de caso a hansen?ase na cidade de Mossor? RN. Na an?lise, ? priorizada a utiliza??o do sistema R, disponibilizando-se scripts de rotinas e procedimentos de an?lise desenvolvidos pelo autor que podem ser facilmente utilizados por pesquisadores de diversas ?reas. Como resultados pr?ticos, importantes ?reas de risco da hansen?ase em Mossor? foram detectadas e sua forte associa??o com o perfil socioecon?mico da popula??o sob risco foi constatada. Al?m disso, estima-se que este trabalho possa vir a incrementar a utiliza??o de tais t?cnicas na an?lise de dados na ?rea de sa?de

Made available in DSpace on 2014-12-17T14:17:54Z (GMT). No. of bitstreams: 1 AdnaLOLF_DISSERT.pdf: 936304 bytes, checksum: 645a4e30c5be1b2d7ff3581233d1e56e (MD5) Previous issue date: 2014-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Seed germination and seedling establishment are critical processes for commercial plantation and depend directly on reserve mobilization as a source of cellular fuels and biosynthetic precursors. In this way, we investigated the coordination among reserve mobilization, metabolite partitioning, and mobilizing enzyme activities in Moringa oleifera Lam (moringa) an oil-seeded species employed in biofuel production. Seeds were germinated under controlled conditions and seedlings were grown hydroponically at a greenhouse. Samples were harvested at 0, 4, 8, 10, 12, 16, and 20 days after imbibition (DAI). The contents of dry mass (DM), neutral lipids (NL), soluble proteins (SP), starch, total soluble sugars (TSS), non-reducing sugars (NRS), and total free amino acids (TFAA) as the activity of isocitrate lyase (ICL), acid proteases, and amylases were determined. The mobilization of storage proteins was initiated during seed germination whereas the mobilization of storage lipids and starch was triggered throughout seedling establishment although all reserves have been depleted until 20 DAI. The partitioning of DM and metabolites to the roots and the shoots was uneven during seedling establishment. Low shoot/root ratio on the basis of DM could be related to the natural occurrence of moringa in drought climates. In the roots, TSS, NRS, and TFAA were accumulated from 12 to 16 DAI and then were consumed until the end of the experiment. In the shoots, TSS and TFAA were consumed in parallel with NRS accumulation from 12 to 20 DAI. The activity of ICL, acid proteases, and amylases was coordinated with the mobilization of lipids, proteins and starch respectively. Thus, we propose that the patterns of reserve mobilization and metabolite partitioning verified in moringa seem distinct from those found to other tree species and may be involved in metabolic strategies to enable environment colonization / A germina??o da semente e o estabelecimento da pl?ntula s?o processos cr?ticos para o cultivo comercial. Assim, este trabalho tem como objetivo caracterizar a mobiliza??o das reservas e a parti??o dos produtos durante estes processos em moringa, uma esp?cie promissora para a produ??o de biodiesel no Nordeste Brasileiro. Para tanto, as sementes foram germinadas em sistema de rolo sob condi??es controladas (80 ?mol/m2/s, fotoper?odo de 12h e 27?2 ?C) e as pl?ntulas foram transferidas para ?gua destilada em vasos pl?sticos de 1 L de capacidade e mantidas em condi??es de casa de vegeta??o por mais 10 dias. As coletas foram realizadas aos 0, 4, 8, 10, 12, 16 e 20 dias ap?s a embebi??o (DAE), dividindo as pl?ntulas em cotil?dones, parte a?rea e sistema radicular. Foram determinados os conte?dos de massa seca (MS), lip?deos neutros (LN), prote?nas sol?veis (PS), amido, a??cares sol?veis totais (AST), a??cares n?o redutores (ANR) e amino?cidos livres totais (AALT) e as atividades de isocitrato liase (ICL), proteases ?cidas e amilases. A parti??o dos produtos, em termos de MS, ocorreu de forma diferencial, resultando em baixa raz?o parte a?rea/sistema radicular, indicativa de um poss?vel mecanismo de resist?ncia ao d?ficit h?drico. A mobiliza??o das PS foi iniciada na germina??o, enquanto que a mobiliza??o dos LN e do amido foi desencadeada no estabelecimento da pl?ntula, embora todas as reservas tenham sido exauridas at? o 20? DAE, culminando com a expans?o das primeiras folhas. No sistema radicular, houve acumula??o de AST, ANR e AALT do 12? ao 16? DAE e consumo destes metab?litos at? o 20? DAE, enquanto que na parte a?rea, ocorreu diminui??o de AST e AALT em paralelo com o aumento de ANR do 12? ao 20? DAE. As atividades de ICL, proteases ?cidas e amilases ocorreram de forma coordenada com a mobiliza??o de LN, PS e amido, respectivamente. Estes padr?es de mobiliza??o das reservas e parti??o dos metab?litos em moringa se mostraram distintos daqueles verificados para outras esp?cies arb?reas estudadas. ? poss?vel que estes padr?es estejam relacionados com estrat?gias metab?licas utilizadas por esta esp?cie para alcan?ar sucesso durante o estabelecimento da pl?ntula

Made available in DSpace on 2014-12-17T14:36:10Z (GMT). No. of bitstreams: 1 IvanilkaLA_TESE_1_a_150.pdf: 4520585 bytes, checksum: 18c2643e49e2eac92f9fe5befa225c45 (MD5) Previous issue date: 2010-05-20 / This thesis represents a didactic research linked to the Post-graduation Programme in Education of the Universidade Federal do Rio Grande do Norte which aimed to approach the construction of the geometrical concepts of Volume of the Rectangular Parallelepiped, Area and Perimeter of the Rectangle adding a study of the Area of the Circle. The research was developed along with students from the 6th level of the Elementary School, in a public school in Natal/RN. The pedagogical intervention was made up of three moments: application of a diagnostic evaluation, instrument that enabled the creation of the teaching module by showing the level of the geometry knowledge of the students; introduction of a Teaching Module by Activities aiming to propose a reflexive didactic routing directed to the conceptual construction because we believed that such an approach would favor the consolidation of the learning process by becoming significant to the apprentice, and the accomplishment of a Final Evaluation through which we established a comparison of the results obtained before and after the teaching intervention. The data gathered were analyzed qualitatively by means of a study of understanding categories of mathematical concepts, in addition to using descriptive statistics under the quantitative aspect. Based on the theory of Richard Skemp, about categorization of mathematical knowledge, in the levels of Relational and Instrumental Understanding were achieved in contextual situations and varied proportions, thus enabling a contribution in the learning of the geometrical concepts studied along with the students who took part in the research. We believe that this work may contribute with reflections about the learning processes, a concern which remained during all the stages of the research, and also that the technical competence along with the knowledge about the constructivist theory will condition the implementation of a new dynamics to the teaching and learning processes. We hope that the present research work may add some contribution to the teaching practice in the context of the teaching of Mathematics for the intermediate levels of the Elementary School / Esta tese se constituiu em uma pesquisa did?tica vinculada ao Programa de P?s-gradua??o em Educa??o da Universidade Federal do Rio Grande do Norte que teve por objetivo abordar ? constru??o dos conceitos geom?tricos de volume do bloco retangular sua ?rea de superf?cie acrescentando um estudo sobre ?rea do c?rculo. Este foi desenvolvido junto a alunos do 6? ano do Ensino Fundamental, em uma escola da rede p?blica de ensino em Natal/RN. A interven??o pedag?gica constou de tr?s momentos: aplica??o de uma avalia??o diagn?stica, instrumento que subsidiou a elabora??o do m?dulo e ensino, ao indicar os conhecimentos pr?vios e fragilidades dos alunos; introdu??o do m?dulo de ensino por atividades e realiza??o de uma avalia??o final atrav?s da qual estabelecemos uma compara??o dos resultados obtidos antes e depois da interven??o de ensino. Os dados obtidos foram analisados qualitativamente mediante estudo de categorias de compreens?o de conceitos matem?ticos, al?m de utilizarmos a an?lise estat?stica validando-a sob o aspecto quantitativo. Com base nos resultados os n?veis de compreens?o relacional e instrumental foram atingidos em situa??es contextuais e propor??es variadas. Acreditamos que este possa contribuir ainda com reflex?es acerca dos processos do conhecer, preocupa??o que permeou todas as etapas da pesquisa. Nossa esperan?a ? que esta pesquisa possa acrescentar alguma contribui??o ao fazer docente no contexto do ensino matem?tico, haja vista concebermos que a compet?ncia t?cnica aliada ao conhecimento sobre a teoria construtivista implementaria uma nova din?mica aos processos de ensino e aprendizagem

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-09T00:26:04Z No. of bitstreams: 1 StefanoPupeJohann_TESE.pdf: 36592987 bytes, checksum: f44357f3110776cf9f7cb628b2d65a95 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-09T00:36:11Z (GMT) No. of bitstreams: 1 StefanoPupeJohann_TESE.pdf: 36592987 bytes, checksum: f44357f3110776cf9f7cb628b2d65a95 (MD5) / Made available in DSpace on 2016-04-09T00:36:12Z (GMT). No. of bitstreams: 1 StefanoPupeJohann_TESE.pdf: 36592987 bytes, checksum: f44357f3110776cf9f7cb628b2d65a95 (MD5) Previous issue date: 2015-03-20 / Os neurocientistas tem uma diversidade de perspectivas com as quais podem classificar diferentes partes do c?rebro. Com o surgimento de t?cnicas baseadas na gen?tica, como a optogen?tica, se torna cada vez mais importante identificar se um grupo de c?lulas, definidas atrav?s de morfologia, fun??o ou posi??o anat?mica possui um padr?o caracter?stico de express?o de um ou mais promotores gen?ticos. Isso permitiria melhores formas de estudar essas popula??es de neur?nios definidas geneticamente. Neste trabalho, eu apresento uma discuss?o te?rica e tr?s estudos experimentais nos quais essa foi a principal quest?o sendo abordada. O Estudo I discute as quest?es envolvidas em selecionar um promotor para estudar estruturas e subpopula??es na ?rea Tegmental Ventral. O Estudo II caracteriza uma subpopula??o de c?lulas na ?rea Tegmental Ventral que compartilha a express?o de um promotor, que ? anatomicamente muito restrita, e que induz avers?o quando estimulada. O Estudo II utiliza uma estrat?gia similar para investigar a subpopula??o no n?cleo subtal?mico que expressa PITX2 e VGLUT2 que, quando inativada, causa hiperlocomo??o. O Estudo IV explora o fato de que um grupo de c?lulas previamente identificadas no Hipocampo Ventral expressa CHRNA2, e indica que essa subpopula??o pode ser necess?ria e suficiente para o estabelecimento do ritmo teta (2-8 Hz) no Hipocampo Ventral de camundongos anestesiados. Todos esses estudos foram guiados pela mesma estrat?gia de identificar um promotor gen?tico capaz de permitir o controle de uma popula??o de neur?nios identificada geneticamente, e eles demonstram as diferentes formas em que essa abordagem pode generar novas descobertas. / Neuroscientists have a variety of perspectives with which to classify different parts of the brain. With the rise of genetic-based techniques such as optogenetics, it is increasingly important to identify whether a group of cells, defined by morphology, function or anatomical location possesses a distinct pattern of expression of one or more genetic promoters. This would allow for better ways to study of these genetically defined subpopulations of neurons. In this work, I present a theoretical discussion and threeexperimental studies in which this was the main question being addressed. Paper I discusses the issues involved in selecting a promoter to study structures and subpopulations in the Ventral Tegmental Area. Paper II characterizes a subpopulation of cells in the Ventral Tegmental Area that shares the expression of a promoter and is anatomically very restricted, and induces aversion when stimulated. Paper III utilizes a similar strategy to investigate a subpopulation in the subthalamic nucleus that expresses PITX2 and VGLUT2 which, when inactivated, causes hyperlocomotion. Paper IV exploits the fact that a previously identified group of cells in the ventral hippocampus expresses CHRNA2, and indicates that this population may be necessary and sufficient for the establishment of the theta rhythm (2-8 Hz) in the Local Field Potential of anesthetized mice. All of these studies were guided by the same strategy of characterizing and studying the role of a genetically defined subpopulation of cells, and they demonstrate the different ways in which this approach can generate new discoveries.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-05-30T22:46:56Z No. of bitstreams: 1 TammsMariaDaConceicaoMoraisCampos_TESE.pdf: 5729452 bytes, checksum: 8c2ea91ababa701fd90228eacee44eb4 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-06-01T21:48:37Z (GMT) No. of bitstreams: 1 TammsMariaDaConceicaoMoraisCampos_TESE.pdf: 5729452 bytes, checksum: 8c2ea91ababa701fd90228eacee44eb4 (MD5) / Made available in DSpace on 2016-06-01T21:48:37Z (GMT). No. of bitstreams: 1 TammsMariaDaConceicaoMoraisCampos_TESE.pdf: 5729452 bytes, checksum: 8c2ea91ababa701fd90228eacee44eb4 (MD5) Previous issue date: 2015-06-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Nas ?ltimas d?cadas a Regi?o Metropolitana de Natal (RMN), assim como em grande parte do Brasil, viveu um aumento do contingente populacional nas cidades e um crescente processo de urbaniza??o com padr?o descont?nuo de expans?o da mancha urbana. A tend?ncia ao maior crescimento demogr?fico nas ?reas periurbanas em expans?o ocasionou novas formas da produ??o do espa?o. A ?vida procura por solo apto a implanta??o de moradias pelo mercado imobili?rio, est? consolidando processos anteriores e gerando novas variantes na ocupa??o dos tecidos metropolitanos. Este fato nos leva a questionar: como a urbaniza??o promovida pela din?mica imobili?ria residencial, no per?odo de 2009-2014, se materializa na (re) configura??o da RMN? Quais as formas e fen?menos territoriais se evidenciam neste processo de ocupa??o do solo metropolitano? De que maneira a intensidade da produ??o habitacional via Programa Minha Casa Minha Vida (PMCMV) se insere no redesenho da atual estrutura f?sica da RMN? Buscar entender a l?gica ocupacional e o processo de espraiamento da expans?o urbana por meio da produ??o recente de moradias no espa?o urbano, contribuindo para a discuss?o de um modelo explicativo de configura??o territorial da RMN ? o objetivo geral desta tese. O territ?rio metropolitano foi analisado em duas escalas: a intraurbana, constitu?da pelos munic?pios de Natal, Parnamirim e S?o Gon?alo do Amarante, enquadrados nesta pesquisa como ?rea Metropolitana Funcional; a interurbana, levando em considera??o todos os munic?pios da RMN. Ambas as escalas foram analisadas buscando os momentos e condi??es gerais que ao longo da hist?ria do processo de metropoliza??o da RMN constitu?das por interven??es por parte do Estado e do mercado imobili?rio, que delinearam a fragmenta??o dos tecidos urbanos e as desigualdades socioespaciais por meio do processo que aqui est? sendo denominado por ?Urbaniza??o Imobili?ria Residencial?. / In the last decades the Natal Metropolitan Area (NMR), as well as in much of Brazil, experienced a population and spatial growth in its cities through/experiencing an urban sprawl pattern of expansion. The trend to intensive growth in peri-urban areas led to new forms of production of space. The real estate search for available land for housing production, are consolidating old processes and generating new patterns in the metropolitan land fabric. This leads us to question: how is the result of the residential real estate dynamic in the (re) configuration of the NMR? What forms and territorial phenomena are evident in this spatial process of the metro area? How much the Minha Casa Minha Vida (MCMV) housing politic is responsible for the spatial changes of NMR? This research aims to understand the logic and the urban sprawl process analyzing/through the recent production of social housing, contributing with an explanatory model of spatial configuration of the NMR. The metro area was analyzed on two scales: the urban, including Natal, Parnamirim and S?o Gon?alo do Amarante cities, framed in this research as Functional Metropolitan Area; intercities, taking into consideration all the municipalities of NMR. In both scales there were cronologically observed the general conditions of the NMR metro expansion throught the state and the real estate interventions, that resulted into the fragmentation of the urban fabric and socio-spatial inequalities what we called here as ?Urbaniza??o Imobili?ria Residencial?.