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Made available in DSpace on 2016-06-02T20:34:49Z (GMT). No. of bitstreams: 1 5686.pdf: 10016184 bytes, checksum: e6a42772f0db7983f4cc9525851f9c5b (MD5) Previous issue date: 2014-01-17 / Universidade Federal de Sao Carlos / O foco principal desse trabalho foi a implementação, otimização e aplicações práticas de métodos de ressonância magnética nuclear (RMN) com o propósito de avaliar as interações entre moléculas de diferentes massas molares, sendo que essas técnicas foram implementadas pela primeira vez no laboratório de RMN do DQ-UFSCar. Existem várias abordagens que podem ser utilizadas com esse propósito, e dentre elas, destacamos: STD NMR, Tr-NOESY, WaterLOGSY, SALMON, INPHARMA, DOSY, SAR. Esses métodos são muito úteis para detectar mudanças de comportamento, a nível molecular, quando adicionamos macromoléculas em um meio contendo somente micromoléculas. O entendimento desse comportamento molecular ajuda a desvendar sistemas complexos de interações moleculares existentes no corpo humano, e que, são muito importantes para o descobrimento de novos medicamentos. O primeiro passo para a implementação das técnicas foi a utilização da proteína de soro bovino (do inglês BSA) e proteína de soro humano (do inglês HSA) como fonte de macromoléculas e micromoléculas orgânicas isoladas da fração etanólica do extrato bruto (1 mg) de Rauia resinous e da fração acetato de etila de Strypnodendron polyphyllum, utilizando cromatografia líquida de alta eficiência, extração por fase sólida e a ressonância magnética nuclear (CLAE-EFS/RMN) para a completa elucidação estrutural quando necessário. As técnicas utilizadas foram: saturation transfer difference (STD), transfer nuclear Overhauser spectroscopy (Tr-NOESY) e STD-TOCSY (total correlation spectroscopy). Essa mesma metodologia além de representar um importante mecanismo para avaliar as interações entre moléculas, também pode ser utilizada para outras matrizes variando tanto as macro quanto as micromoléculas. / The main focus of this work was the implementation, optimization and practical applications of nuclear magnetic resonance (NMR) methods for the purpose of evaluating the interactions between molecules of different masses, and these techniques were implemented for the first time in the laboratory NMR DQ - UFSCAR. There are a number of ligand-based screening approaches available, such that, STD NMR, Tr-NOESY, WaterLOGSY, SALMON, INPHARMA, DOSY, SAR by NMR etc. These methods are sensitive towards the perturbations as results of the macromolecular addition in a medium containing the small molecules. The molecular understanding of this behavior helps to uncover the complex systems of molecular interactions existing in the human body, which are very important for the discovery of new medicines. In the first step while implementing these techniques, Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) as a source of organic macromolecules were used. While, for the small organic molecules, a 1 mg crude extract from the hydroethanolic fraction of the Rauia resinous and ethyl acetate fraction of Strypnodendron polyphyllum was utilized, however, for the complete structural characterization, solid phase extraction following high-pressure liquid chromatography in an integrated fashion and then nuclear magnetic resonance (LC-SPE/NMR) was employed when necessary. On the other hand, the ligand screening techniques used were the saturation transfer difference (STD), Nuclear Overhauser Transfer Effect SpectroscopY (Tr- NOESY), Diffusion-Order Spectroscopy (DOSY) and STD- TOCSY (TOtal Correlation SpectroscopY). More importantly, this methodology also represents an important mechanism to evaluate the interactions between molecules or the first hand detection of the active constituents/inhibitors, which can also be used for other matrices varying both the macro and the small molecules.

En s'appuyant sur une connaissance précise du temps, sur la réservation de ressources et l'application distribuée de règles d'admission strictes, un réseau déterministe permet de transporter des flux pré-spécifiés avec un taux de perte extrêmement bas et une latence maximale majorée, ouvrant la voie au support d'applications critiques et/ou temps-réel sur une infrastructure de réseau convergée. De nos jours, la Technologie Opérationnelle (OT) s'appuie sur des réseaux déterministes mais conçus à façon, en général propriétaires, utilisant typiquement des liens série spécifiques, et opérés en isolation les uns des autres, ce qui multiplie la complexité physique et les coûts d'achat et de déploiement (CAPEX), ainsi que d'opération et maintenance (OPEX), et empêche l'utilisation agile des ressources. En apportant le déterminisme dans les réseaux des Technologies de l'Information (IT), une nouvelle génération de réseaux commutés de l'IT va permettre l'émulation de ces liens série et la convergence de réseaux autrefois dédiés sur une infrastructure commune à base d'IP. En retour, la convergence de l'IT et de l'OT permettra de nouvelles optimisations industrielles, en introduisant des technologies héritées de l'IT, comme le BigData et la virtualisation des fonctions du réseau (NFV), en support des opérations de l'OT, améliorant les rendements tout en apportant une réduction supplémentaire des coûts. Les solutions de réseaux déterministes réclament des possibilités nouvelles de la part des équipements, possibilités qui vont bien au-delà de celles demandées pour les besoins classiques de la QoS. Les attributs-clé sont : - la synchronisation précise de tous les n'uds, en incluant souvent la source et la destination des flux- le calcul centralisé de chemins de bout en bout à l'échelle du réseau- de nouveaux filtres de mise en forme du trafic à l'intérieur comme à l'entrée du réseau afin de le protéger en tous points- des moyens matériels permettant l'accès au medium à des échéances précises. Au travers de multiples papiers, de contributions à des standards, et de publication de propriété industrielle, le travail présenté ici repousse les limites des réseaux industriels sans fils en offrant : 1. Le calcul centralisé de chemin complexes basé sur une technologie innovante appelée ARC 2. La signalisation de ces chemins complexes et la traçabilité des paquets par une extension de la technologie BIER-TE 3. Réplication, Renvoi et Elimination des doublons le long de ces chemins complexes 4. Un temps-réel basé sur un échéancier qui assure un haut taux de délivrance et garantit une latence bornée 5. La capacité de transporter à la fois des flux déterministes et du trafic IPv6 à multiplexage statistique sur un maillage 6TiSCH partagéCe manuscrit rapporte des améliorations apportées aux techniques existantes des réseaux sans fils à basse puissance (LoWPAN) comme Zigbee, WirelessHART'et ISA100.11a, afin d'amener ces nouveaux bénéfices jusqu'aux réseaux opérationnels sans fil. Elle a été implémentée en programme et sur du matériel open-source, et évaluée face à du IEEE Std. 802.15.4 classique ainsi que du 802.15.4 TSCH, utilisés en topologie maillée. L'expérience menée montre que notre nouvelle proposition permet d'éviter les à-coups et de garantir des taux élevés de délivrance, même face à des évènements exceptionnels comme la perte d'un relais ou la dégradation temporaire d'un lien radio. / Based on time, resource reservation, and policy enforcement by distributed shapers, Deterministic Networking provides the capability to carry specified unicast or multicast data streams for real-time applications with extremely low data loss rates and bounded latency, so as to support time-sensitive and mission-critical applications on a converged enterprise infrastructure.As of today, deterministic Operational Technology (OT) networks are purpose-built, mostly proprietary, typically using serial point-to-point wires, and operated as physically separate networks, which multiplies the complexity of the physical layout and the operational (OPEX) and capital (CAPEX) expenditures, while preventing the agile reuse of the compute and network resources.Bringing determinism in Information Technology (IT) networks will enable the emulation of those legacy serial wires over IT fabrics and the convergence of mission-specific OT networks onto IP. The IT/OT convergence onto Deterministic Networks will in turn enable new process optimization by introducing IT capabilities, such as the Big Data and the network functions virtualization (NFV), improving OT processes while further reducing the associated OPEX.Deterministic Networking Solutions and application use-cases require capabilities of the converged network that is beyond existing QOS mechanisms.Key attributes of Deterministic Networking are: - Time synchronization on all the nodes, often including source and destination - The centralized computation of network-wide deterministic paths - New traffic shapers within and at the edge to protect the network- Hardware for scheduled access to the media.Through multiple papers, standard contribution and Intellectual Property publication, the presented work pushes the limits of wireless industrial standards by providing: 1. Complex Track computation based on a novel ARC technology 2. Complex Track signaling and traceability, extending the IETF BIER-TE technology 3. Replication, Retry and Duplicate Elimination along the Track 4. Scheduled runtime enabling highly reliable delivery within bounded time 5. Mix of IPv6 best effort traffic and deterministic flows within a shared 6TiSCH mesh structureThis manuscript presents enhancements to existing low power wireless networks (LoWPAN) such as Zigbee, WirelessHART¿and ISA100.11a to provide those new benefits to wireless OT networks. It was implemented on open-source software and hardware, and evaluated against classical IEEE Std. 802.15.4 and 802.15.4 TSCH radio meshes. This manuscript presents and discusses the experimental results; the experiments show that the proposed technology can guarantee continuous high levels of timely delivery in the face of adverse events such as device loss and transient radio link down.

Orientador: Ljubica Tasic / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Química / Made available in DSpace on 2018-08-20T16:55:17Z (GMT). No. of bitstreams: 1 Prando_Alessandra_D.pdf: 9977861 bytes, checksum: 15d25bce7d95433f95b938e68fdeaac8 (MD5) Previous issue date: 2012 / Resumo: Até o momento, pouco se sabe sobre os mecanismos de virulência da bactéria Xanthomonas axonopodis pv. citri (XAC), agente causador do cancro cítrico. Acredita-se que chaperonas de secreção (CS) estão envolvidas no processo de patogenicidade de XAC primeiramente formando complexos com fatores de virulência e auxiliando no encaminhamento desses para os sistemas de secreção utilizando o ATP como fonte de energia. Neste trabalho foram adquiridos dados de fluorescência de emissão, dicroísmo circular, desenovelamento térmico e de ressonância magnética nuclear de H NMR e de 2D {N,H} HSQC de duas proteínas da XAC, a XAC1990 (FlgN) e XACb0033. Para ambas proteínas foram propostas estruturas 3D usando a análise de footprinting com restrições SASA e rmsd. Para as estruturas propostas foi verificado que os dados de fluorescência corroboram com a estrutura 3D não ocorrendo o mesmo para os dados de CD e NMR que revelaram baixo conteúdo helicoidal além de ausência de estrutura 3 D. A interação da proteína FlgN com a sua proteína parceira FlgK também foi sugerida através das análises de CD e fluorescência. Na segunda parte do trabalho foram estudadas as interações entre a proteína Hsp90 da laranja com diferentes ligantes aplicando a técnica de Saturation Transfer Difference (STD-NMR) e espectroscopia de fluorescência. Estas análises revelaram dados que corroboraram com o modelo proposto e, além disso, indicaram que os hidrogênios H-8 e H-2 da adenina e H-1'da ribose estão localizados no sítio ligante da proteína com os fosfatos orientados para fora. Através da fluorescência foram calculados os valores de Kd e foi verificado que a geldanamicina é um potente inibidor de Hsp90 da laranja / Abstract: So far, the Xanthomonas axonopodis pv. citri (XAC) mechanisms of bacterial virulence is unknown. It is believed that secretion chaperones (CS) are involved in the XAC's virulence process by first forming complexes with virulence factors, and assisting in their presentation to corresponding secretion systems using ATP as a source of energy. Fluorescence emission, circular dichroism, thermal unfolding and nuclear magnetic resonance NMR H and 2D {N,H} HSQC data from two proteins of XAC, XAC1990 (FlgN) and XACb0033 were collected. For both proteins, 3D structures were proposed using the footprinting analysis with RMSD and SASA restrictions. For the proposed structures were verified which the fluorescence data were consistent with the 3D structure. The CD and NMR data revealed low-helical content and absence of 3D structure. The interaction of the protein FlgN with its partner, FlgK, was suggested by CD and fluorescence analysis. In the second part, the interactions between the orange's Hsp90 protein with different ligants using Saturation Transfer Difference (STD-NMR) and fluorescence spectroscopy techniques were studied. These analyzes revealed which the data were consistent with the proposed model and moreover showed that the adenine's hydrogens H-8 and H-2 and ribose's hydrogen H-1'are located in the protein binding site with the phosphate driven out. By fluorescence values were calculed Kd and it was verified that geldanamycin is a potent inhibitor of orange's Hsp90 / Doutorado / Quimica Organica / Doutor em Ciências

Abstract This thesis studies the methods to test mixed-signal devices and circuits on board and system level with embedded test instrumentation. The study is divided in three continuous sections, development of embedded test methods for discrete components, integration of test instruments on board level and development of test and health monitoring strategy for large scale system. The developed embedded test methods for mixed signal circuitry on board level are based on the standard for mixed signal test bus IEEE 1149.4. The standardized embedded test infrastructure is utilized for testing discrete components with emphasis on testing active components as diodes and transistors. The developed embedded tests are evaluated with PCOLA/SOQ method for manufacturing testing and also the usability of the tests is discussed. A solution for embedded mixed-signal test controller is presented with discussion of test communication and the possibilities of implementing embedded test control. The target in the development of the test control is to enable launch mixed signal tests on device remotely. The test controller is IEEE 1149.4 compatible and can generate and measure analog test signals while controlling boundary-scan enabled devices. The final section of the thesis focuses on an embedded test solution for aerospace bus system (MIL-STD-1553). Current solutions are based on testing the bus system during maintenance on ground. The developed test and monitoring method allows on-line monitoring of the bus to detect and locate possible defects which only occur during use of the aeroplane. / Tiivistelmä Väitöstyössä tutkittiin sekasignaalilaitteiden ja -piirien testausmenetelmiä levy- ja järjestelmätasolla hyödyntäen sulautettuja testilaitteita. Työ jakaantuu kolmeen osaan; sulautettujen testausmenetelmien kehitys diskreeteille komponenteille, testi-instrumenttien integrointi piirilevytasolle sekä testaus- ja kunnonmonitorointimenetelmän kehitys laajemmalle järjestelmälle. Sulautettujen testimenetelmien kehitys sekasignaalipiireille piirilevytasolla perustuu sekasignaalitestiväylän standardiin IEEE 1149.4. Standardoitua sulautettua testi-infrastruktuuria käytettiin diskreettien komponenttien testaukseen painottuen aktiivikomponentteihin, kuten diodeihin ja transistoreihin. Kehitetyt sulautetut testit on arvioitu PCOLA/SOQ menetelmällä, jota hyödynnetään tuotantotestauksen testikattavuuden arvioinnissa. Lisäksi testimenetelmien käytettävyyttä arvioitiin. Sulautettu sekasignaalilaitteiden testikontrollerin tavoite on käynnistää ja suorittaa sekasignaalitestejä laitteessa etäältä. Kehitetty testikontrolleri on IEEE 1149.4 yhteensopiva ja voi generoida ja mitata analogista testisignaalia sekä samanaikaisesti ohjata testiväylää. Lisäksi etätestauksen mahdollistavasta testikommunikaatiomenetelmiä arvioitiin kuten myös erilaisia toteutustasoja sulautetuille testimenetelmille. Laajemman järjestelmän kehityksessä tutkittiin sulautettua testausratkaisua lentokoneen väyläjärjestelmälle, joka perustuu standardiin MIL-STD-1553B. Nykyiset menetelmät perustuvat väyläjärjestelmän testaukseen huollon yhteydessä, mutta osa virheistä ilmenee vain käytön aikana. Kehitetty testaus- ja monitorointimenetelmä mahdollistaa käytönaikaisen jatkuvan virheiden monitoroinnin sekä niiden paikantamisen lennon aikana.

The research reported in this thesis is an extension and application of the results first introduced by the Procedural Approach to the Evaluation of Software Development Methodologies. The evaluation procedure offers a unique perspective based on the philosophy that a software development methodology should espouse a set of objectives that are achieved by employing certain U principles throughout the software development process, such that the products generated possess certain attributes deemed desirable. Further, definite linkages exist between objectives and principles, and principles and attributes. The work described herein adopts the perspective offered by the evaluation procedure and applies a critical analysis to the process-product relationships in DoD-STD-2l67 and DoD-STD-2l68. In support of the analysis, Augmented Data Flow Diagrams are introduced as an effective tool for concisely capturing the information in both standards. The results of the analysis offer a deeper insight into the requirements for defense system software development, such that one is able to better understand the development process, and more intelligently assess the quality of the software and documentation produced. / Master of Science

ITC/USA 2010 Conference Proceedings / The Forty-Sixth Annual International Telemetering Conference and Technical Exhibition / October 25-28, 2010 / Town and Country Resort & Convention Center, San Diego, California / Modern data acquisition systems can be very time consuming to configure. The most time consuming aspect of configuring a data acquisition system is defining the measurements that the system will collect. Each measurement has to be uniquely identified in the system and the system needs to know what data the measurement will sample. Data acquisition systems are capable of sampling thousands of measurements in a single test flight. If all of the measurements are created by hand, it can take many hours to input all of the required measurements into the data acquisition system's setup software. This process can also be extremely tedious since many measurements are very similar. This paper will examine several possible solutions to the problem of rapidly creating large numbers of data acquisition measurements. If the list of measurements that need to be created already exists in an electronic format then the simplest approach would be to create an importer. The two main ways to import data are XML and comma separated value files. This paper will discuss the advantages and disadvantages of both approaches. In addition to importers, this paper will discuss a system that can be used to create large numbers of similar measurements very quickly. This system is ideally suited to MILSTD- 1553 and ARINC-429 bus data. It exploits the fact that most bus measurements are typically very similar to each other. For example, 1553 measurements typically differ only in terms of the command word and the selected data words. This system allows the user to specify ranges of data words for each command word. It can then create the measurements based on the user specified ranges.

International Telemetering Conference Proceedings / October 25-28, 1993 / Riviera Hotel and Convention Center, Las Vegas, Nevada / DOD flight test centers need affordable, small-format, flight-qualified digital instrumentation recording solutions to support existing and future flight testing. The Advanced Airborne Test Instrumentation System (AATIS) is today's primary data acquisition system at the Air Force Flight Test Center (AFFTC). Digital Recorder (DR) 1995 is planned to provide full support for AATIS output capabilities and satisfy initial recording requirements for the Common Airborne Instrumentation System (CAIS). The follow-on to the AATIS, the CAIS is a tri-service development to satisfy future DOD flight test data acquisition requirements. DR 2000 is planned as the future recording solution for CAIS and will be able to fully satisfy the 50 Mbps recording requirement. In the developments of DR 1995 and DR 2000, commonality and interoperability have emerged as significant issues. This paper presents an overview of these recording solutions and examines commonality and interoperability issues.

International Telemetering Conference Proceedings / October 29-November 02, 1990 / Riviera Hotel and Convention Center, Las Vegas, Nevada / Increased data throughput demands in military and avionics systems has led to the development of an advanced, All-Bus MIL-STD-1553 Instrumentation Monitor. This paper discusses an airborne unit which acquires the information from up to 8 dual-redundant buses, and formats the data for telemetry, recording or real-time analysis according to the requirements of IRIG-106-86, Chapter 8. The ALBUS-1553 acquires all or selected 1553 messages which are formatted into IRIG-compatible serial data stream outputs. Data is time tagged to microsecond resolution. The unit selectively transmits entire or partial 1553 messages under program control. This results in reduced transmission bandwidth if prior knowledge of 1553 traffic is known. The ALBUS also encodes analog voice inputs, discrete userword inputs and multiplexed analog (overhead) inputs. The unit is provided in a ruggedized airborne housing utilizing standard ATR packaging,

International Telemetering Conference Proceedings / October 30-November 02, 1995 / Riviera Hotel, Las Vegas, Nevada / The MARS-II digital recorder is one of the new technologies that will eventually replace the labor intensive and hardware dependent methods associated with traditional analog-based telemetry ground systems. The Standardized MARS-II Analysis and Reduction Tool (SMART) is one of the first software systems developed to take advantage of this new digital recording capability. It processes pulse code modulated (PCM) encoded data and MIL-STD-1553B message traffic, outputting time-tagged PCM frames or 1553 messages to file. The goal of this software is to provide a portable application that utilizes state-ofhe-art, general purpose hardware for rapid telemetry data processing to meet the needs of operational users, telemetry engineers, and data analysts. To satisfy these goals, the software was developed using the C language with VMS and OSF operating systems as the initially targeted platforms. In addition, an X Window System/Motif graphical user interface supporting three tiers of user interaction (operator, telemetry engineer, and telemetry analyst) was layered onto the decommutator functions.