Cyclic and extreme ecological disturbances have the capacity to alter resources and thereby animal populations. Interactions between disturbance and resource availability can influence predator-prey interactions. Predator-prey responses to ecological disturbance may be more pronounced in herbivores and their predators as herbivores track food resources that are often augmented by ecological disturbance. My objective with this dissertation was to examine how various forms of ecological disturbance influence predator-prey interactions through the lens of a case study – white-tailed deer (Odocoileus virginianus) and Florida panther (Puma concolor coryi) in southwestern Florida public and conservation lands. I quantified species-specific behavior of deer to an extreme disturbance event (i.e., Hurricane Irma), examined behavior of females with different fate outcomes to varied ecological disturbances and predation, investigated how ecological disturbance mediates the influence of human disturbance on predator-prey interactions, and quantified deer spatial ecology in response to fire, hydrology, panther and human activity. I found that deer behaviorally mediated the negative fitness impacts of Hurricane Irma. Further, I found that female deer with different fate outcomes selected areas of different ecological disturbance and the ecological disturbance type conferred different fitness costs (through differences in predation risk). Finally, I found that South Florida deer utilize diurnal times when humans are the most active to temporally reduce predation risk as panthers were more nocturnal in response to humans. My work here suggests that ecological disturbance regimes have the capacity to influence predator-prey interactions through nuanced mechanisms. Outcomes of these nuanced species-specific and predator-prey responses should be examined further. More practically, if disturbance influences aspects of animal fitness, a deeper understanding of species-specific and predator-prey responses to disturbance will improve management and conservation efforts as some regimes can be manipulated (e.g., prescribed fire). More broadly, consideration of ecological disturbance when examining predator-prey interactions may yield novel insight that deviates from predictions based on inference suggested in systems without disturbance. Highlighting nuanced predator-prey interactions mediated by ecological disturbances will improve predictions regarding species and community responses to global changes such as climate change and ecological restoration. / Doctor of Philosophy / Events that cause temporary changes to ecosystem structure and function (ecological disturbances) have the capacity to influence resources (i.e., food and shelter) for wild animals. Changes in resources as a function of ecological disturbance has the capacity to influence prey and predator species interactions. Predator-prey responses to ecological disturbance may be more pronounced in plant-eating animals (herbivores) and their predators as herbivores utilize food resources that are often altered by ecological disturbance. My objective with this dissertation was to examine how various forms of ecological disturbance influence interactions between predators and prey by using the white-tailed deer (Odocoileus virginianus) and Florida panther (Puma concolor coryi) in southwestern Florida wildlands as a case study. I quantified species-specific behavior of deer to an extreme climate event (i.e., Hurricane Irma). Next, I quantified and compared behavioral differences in responses to ecological disturbance (flooding and fire) and panther predation risk between female deer that survived and those killed by panthers during the offspring rearing season. I also investigated how ecological disturbance and human use of wildlands influenced predator-prey interactions. Finally, I characterized deer behavior in response to fire, hydrology, and panther and human activity. I found that deer changed their behavior during Hurricane Irma, presumably to offset the negative impacts of the storm as all our monitored deer survived the event. Further, I found that different ecological conditions generated by fire and flooding, respectively, influenced female behavior during the offspring rearing season in response to predation risk, and those behavioral differences may explain differences in mortality outcomes. Finally, I found that South Florida deer utilize daylight hours when humans are the most active to minimize encounters with predators as panthers were shown to be more active at night in areas with greater human use. My findings suggest that ecological disturbances have the capacity to influence predator-prey interactions in novel ways not suggested elsewhere. Outcomes of novel predator and prey interactions in response to ecological disturbance should be investigated further. More practically, if disturbance influences aspects of animal livelihood, a deeper understanding of species-specific and predator-prey responses to disturbance will improve management and conservation efforts as some disturbances can be manipulated (e.g., prescribed fire). More broadly, consideration of ecological disturbance when examining predator-prey interactions may yield novel insight that deviates from predictions based on inference suggested in systems without disturbance. Highlighting novel predator-prey interactions that is changed as a result of ecological disturbances will improve predictions regarding species and community responses to global changes through climate change and ecological restoration.

As the earth’s landscape becomes increasingly urbanized, local wildlife must adapt to urban conditions or migrate to areas that are more rural. Urban wildlife face challenges such as direct loss of habitat, competition with non-native species, disturbance due to anthropogenic noise, and micro-climatic changes. Factors such as temperature, relative humidity, and noise affect the acoustical environment and may affect the ability of many animals, including birds, to communicate. Understanding how urbanization affects birds’ singing behavior is critical because singing often plays a vital role in attracting mates and defending territories. In addition, as global climate change occurs it will become increasingly important to understand the influence of abiotic factors such as temperature on the singing behavior of birds. Developing a deeper understanding of how those abiotic factors affect singing and other associated behaviors may help guide future conservation actions. Carolina Wrens are often used to study the role of singing in defending territories. They are also a common subject for ranging studies. However, surprisingly little research has been conducted on the factors influencing Carolina Wren song activity over the course of a day. No studies have compared the singing activity of Carolina Wrens in urban and rural environments. The purpose of this study was to compare the singing activity of Carolina Wrens in urban and rural areas. Specifically, this study looked for patterns in the timing of songs throughout the day and examined the effects of season, temperature, and anthropogenic noise on singing activity in urban and rural settings. I analyzed data from 4 SongMeter© SM2 data loggers (2 located at an urban park in Bowling Green, KY and 2 located at a rural preserve in Hart County, KY). The data loggers were programmed to record ambient sound for 3 minutes at the beginning of every hour. A Hobo® data logger was deployed with each SongMeter© SM2 data logger to record temperature and relative humidity at 15-minute intervals. One week of data in May and one week of data in July were analyzed. Both weeks had similar photoperiods, temperatures, and amounts of precipitation. For each recording, I documented the number of Carolina Wren songs I heard and a categorical assessment of the precipitation and anthropogenic noise due to traffic or machinery. I conducted Chi-square analyses to compare the seasonal or site-based observed frequencies of singing activity to the corresponding expected frequencies. In May, Carolina Wrens at the urban site sang more than their rural counterparts. Daily activity patterns were visually analyzed using the average number of songs detected for each hour’s sampling period. No Carolina Wrens were heard singing the hour before sunrise or the hour after sunset. In May, the dawn chorus lasted nearly twice as long as in July. Zero-inflated Poisson regressions were used to determine the correlations between Carolina Wren singing activity and temperature or anthropogenic noise. Carolina Wren singing activity had significant correlations with both variables.

O objetivo da presente pesquisa foi fornecer subsídios, visando ao controle de Diatraea saccharalis (Fabricius, 1794) em milho, com o parasitóide de ovos Trichogramma galloi Zucchi, 1988. Foram realizados estudos do parasitóide, incluindo seleção de linhagens, efeito de fatores abióticos (temperatura e alimento) sobre parâmetros biológicos e de parasitismo, bem como comportamento de postura de D. saccharalis em milho. A seleção de linhagens foi conduzida nas temperaturas de 25 e 30°C e o efeito da temperatura sobre a longevidade e a capacidade de parasitismo foi estudado em 15 condições térmicas (na faixa de 10 a 38°C). O efeito de quatro tipos de alimentos foi avaliado sobre a longevidade e a capacidade de parasitismo. O comportamento de postura de D. saccharalis foi observado em dois níveis de infestação (dois e dez casais por planta). Constatou-se que a linhagem G16080 foi a mais adequada para liberações visando ao controle de D. saccharalis, com base na capacidade de parasitismo e na duração do ciclo e por ter apresentado desempenho equivalente na faixa de 25 a 30°C. A maior capacida de de parasitismo de T. galloi linhagem G16080 ocorreu entre 20 e 28°C, embora ten ha havido parasitismo em todas as temperaturas na faixa de 10 a 38ºC. Não houve correlação entre a longevidade e parasitismo, havendo uma concentração deste parasitismo quando o inseto viveu menos. A presença do hospedeiro interferiu na longevidade de T. galloi linhagem G16080. Considerando-se o parasitismo, mel puro e a solução de pólen são os alimentos mais adequados para T. galloi linhagem G16080. Com ou sem o hospedeiro, mel + pólen foi o alimento que proporcionou a maior longevidade para T. galloi linhagem G16080. Independentemente do nível populacional de D. saccharalis, houve uma acentuada preferência pela região do colmo para postura, em relação às folhas de milho de 45 dias de idade, o que pode definir a amostragem e a forma de liberação do parasitóide. / The objective of this study was to provide information aiming to control Diatraea saccharalis (Fabricius, 1794) in corn with the egg parasitoid Trichogramma galloi Zucchi, 1988. Studies realized on the parasitoid included strain selection; effect of abiotic factors (temperature and food) on biological parameters and parasitism as well as oviposition behavior of D. saccharalis in corn. Strain selection was conducted at a temperature of 25 and 30°C and the effect of temper ature on longevity and parasitism capacity was studied at 15 temperature conditions (in the range of 10 to 38°C). The effect of four types of food sources was evaluated on longevity and parasitism capacity. The oviposition behavior of D. saccharalis was observed in two levels of infestation (two and ten couples per plant). It was verified that the strain G16080 was more suitable for release with the aim to control D. saccharalis based on the parasitism capacity and duration of the life cycle and having equivalent performance at a temperature range of 25 to 30°C. The highest rate of parasitism by the T. galloi strain G16080 occurred between 20 and 28°C, althoug h there was parasitism in all other temperature ranges of 10 to 38ºC. There was no correlation between longevity and parasitism, with concentration of this parasitism when the insect lived less. The presence of the host interfered with longevity of the T. galloi strain G16080. Considering parasitism, pure honey and the pollen solution are more suitable as food for T. galloi strain G16080. With or without the host, honey + pollen was the food that produced the highest longevity for T. galloi strain G16080. Irrespective of the population level of D. saccharalis, there was a clear preference for oviposition on the stem in relation to 45 day old corn leaves that could define sampling and the form of release of the parasitoid.

A utilização de sistemas de bateria de gaiolas é assunto de grande polêmica nos países da Europa, sendo que a maior preocupação com o uso de gaiolas se dá quanto ao espaço oferecido às aves poedeiras, o qual certamente afeta seu bem-estar. Sendo assim este trabalho teve como objetivos avaliar os aspectos relacionados ao comportamento e bem-estar das aves, comparando o sistema de criação convencional (bateria de gaiolas) com um sistema de criação em cama, ninho e poleiro, bem como avaliar a influencia das condições ambientais (estresse e conforto) na qualidade final dos ovos, associando técnicas da zootecnia de precisão (análise de imagens), para avaliar as respostas comportamentais das diferentes linhagens nas diferentes condições. Dois grupos de 20 aves (10 Hy-line W36 e 10 Hy-line Brown) em inicio de postura foram submetidas a duas condições ambientais durante duas semanas consecutivas (26°C e 60% UR e 35°C e 70%UR), sendo também submetidas a dois sistemas de criação (bateria de gaiolas e cama). Durante o período de avaliação um sistema de câmeras de vídeo registrava o comportamento das aves. Os ovos produzidos durante todo o período da pesquisa foram avaliados pela análise dos seguintes parâmetros de qualidade: peso do ovo, espessura da casca, gravidade especifica, unidades Haugh, além de análise da colorimetria da gema, porosidade da casca e análises microbiológicas, para a verificação de uma possível ocorrência de contaminação por Salmonella sp na gema e casca dos ovos. Os resultados obtidos revelaram uma redução significativa (P<0,05) nos parâmetros de qualidade do ovo para a condição de estresse térmico, principalmente para o sistema de criação em gaiolas. Quanto as análises de comportamento, o sistema de criação em cama proporcionou a expressão de todos os comportamentos naturais e de conforto das aves, favorecendo assim melhores condições de bem-estar. Para o sistema de criação convencional (gaiolas) foi possível verificar que mesmo sem nenhuma condição, as aves ainda tentavam executar seus comportamentos naturais, sendo que a impossibilidade de expressarem estes comportamentos certamente agravou a condição de estresse provocada por este sistema de criação. Pela análise dos dois sistemas de criação e das duas condições ambientais a linhagem Hy-Line W36. foi a que obteve um melhor desempenho. / The battery cage system is a very polemic issue in European countries, being the most commonly raised concerns focused on the spatial restrictions of the hens, which might compromise important comfort movements affecting their welfare conditions. This work aimed to evaluate aspects related to behavior and welfare of hens comparing a conventional housing system (laying cages in battery) with another system using litter, lair and roost. In addition, the effects of environmental conditions (stress or comfort) on final egg quality and behavior responses (analyzed by precision techniques) were evaluated in different laying hen lines. Two groups of 20 birds (10 Hyline W36 and 10 Hy-line Brown) at the beginning of production phase were submitted to two environmental conditions (26°C with 60% RU or 35°C with 70% RU) and two housing systems (cages in battery or litter) during two consecutive weeks. During the evaluation period, bird behaviors were recorded by video cameras. Total egg production was analyzed according to egg weight, shell thickness, specific gravidity, Haugh unit, yolk colorimetric parameters and shell porosity. Eggs were microbiologically analyzed in order to detect Salmonella sp contamination in yolk and eggshell. A significant (P<0.05) reduction in quality parameters was observed in eggs from laying hens raised under heat stress, mainly in those from laying cage system. In relation to behavior, the housing system in litters proportioned the expression of all natural behaviors and those related to bird comfort, which permitted to improve welfare conditions. In conventional system (laying cages) was verified that birds tried to manifest their natural behavior in spite of lacking condition for it. This impossibility for executing natural behaviors promoted an increase of stress provoked by the housing system. Analyzing both systems and environmental conditions, hens from Hy-Line W36 showed the better performances.

Os fatores idade e sexo modificam a experiência dolorosa em animais e seres humanos. Os efeitos dos hormônios gonadais têm sido estudados em diversos modelos experimentais de dor, no entanto, o efeito do envelhecimento na percepção à dor carece de mais investigação. O efeito do envelhecimento na dor neuropática ainda não está bem estabelecido. Neste estudo se procurou avaliar possíveis variações na percepção da dor da hiperalgesia mecânica, em função da idade, presença e ausência de hormônios gonadais e sexo em ratos Wistar machos e fêmeas, jovens e idosos no modelo de dor neuropática, ligadura da quinta raiz lombar. Os animais foram divididos nos seguintes grupos: ratos jovens orquiectomizados e não-orquiectomizados, ratos idosos orquiectomizados e não-orquiectomizados, ratas jovens ooforectomizadas com ou sem reposição de 17beta-estradiol e ratas idosas. Foi testado o limiar de retirada da pata após estímulo mecânico antes da ligadura e no 7º, 14º, 21º e 28o dias após a ligadura. Os resultados mostraram que todos os animais apresentaram comportamento hiperalgésico após ligadura da quinta raiz lombar durante os 28 dias de observação. A hiperalgesia ocorreu independentemente do sexo do animal, da presença ou ausência de hormônios gonadais ou idade. Não houve diferença entre ratos jovens e idosos não-orquiectomizados (p = 0,420), entre ratos jovens e idosos orquiectomizados (p = 0,560). Entre os ratos idosos com e sem orquiectomia houve diferença no 14º (p = 0,038) e 28º (p = 0,002) dias. Ratas jovens ooforectomizadas sem reposição de 17beta-estradiol apresentaram menor hiperalgesia que ratas ooforectomizadas com reposição durante todo o período (p = 0,001). Não houve diferença entre ratos idosos orquiectomizados e ratas idosas (p = 0,09). Ratos jovens não-orquiectomizados apresentaram menor hiperalgesia mecânica que ratas jovens ooforectomizadas com reposição de 17beta-estradiol (p = 0,001), o mesmo não ocorreu entre machos e fêmeas jovens gonadectomizados sem reposição hormonal (p = 0,511). Ratas jovens ooforectomizadas sem reposição de 17beta-estradiol e ratas idosas mostraram diferença apenas no 7º dia (p = 0,002). Os resultados permitiram concluir que a reposição de 17beta-estradiol aumentou a hiperalgesia mecânica aos estímulos mecânicos após ligadura da quinta raiz lombar em ratas jovens ooforectomizadas, a hiperalgesia mecânica não diferiu entre os sexos em ratos jovens machos e fêmeas, a presença da gônada masculina diminuiu a hiperalgesia mecânica em ratos idosos e que houve diminuição da hiperalgesia mecânica em ratas idosas / Age and sex modify the pain experience in animals and humans. The effects of gonadal hormones have been studied in various experimental pain models, however, the effect of aging on pain perception needs further investigation. The effect of aging on neuropathic pain is not well established. In this study, we sought to determine how aging and gonadal hormones affect mechanical hyperalgesia using spinal nerve ligation as a neuropathic pain model in aged and young male and female Wistar rats. Animals were divided into seven groups: aged female, ovariectomized young females with 17beta-estradiol replacement, ovariectomized young females without 17beta-estradiol replacement, orchiectomized and non-orchiectomized aged and young males. Rats were tested for mechanical hyperalgesia in the plantar surface of the left hindpaw before nerve ligation and on days 7, 14, 21 and 28 after nerve ligation. All animals of all groups showed mechanical hyperalgesic behavior after spinal nerve ligation during entire period of 28 days. Hyperalgesia was independent of the sex of the animal, the presence or absence of gonadal hormones or age. There was no difference between non-orchiectomized aged and young males (p = 0.420), and between orchiectomized aged and young males (p = 0.560). There was difference between aged male rats with and without orchiectomy in days 14 (p = 0.038) and 28 (p = 0.002). Young ovariectomized female rats without 17beta-estradiol replacement had less hyperalgesia than young ovariectomized female rats with replacement (p = 0.001). There was no difference between aged orchiectomized male rats and old female rats (p = 0.09). Young non-orchiectomized male rats showed less mechanical hyperalgesia than young ovariectomized female rats with 17beta-estradiol replacement (p = 0.001), that did not occur between young orchiectomized males rats and young ovariectomized females rats without 17beta-estradiol replacement (p = 0.51). Young ovariectomized female rats without 17beta-estradiol replacement and old female rats showed differences only on day 7 (p = 0.002). These data suggest that estradiol presented a pronociceptive effect in young female rats in mechanical hyperalgesia in the neuropathic pain model, spinal nerve ligation, withdrawal threshold did not differ between sex, the presence of male gonadal hormones reduces mechanical hyperalgesia in old male rats and there is reduced mechanical hyperalgesia in aged female rats

A utilização de sistemas de bateria de gaiolas é assunto de grande polêmica nos países da Europa, sendo que a maior preocupação com o uso de gaiolas se dá quanto ao espaço oferecido às aves poedeiras, o qual certamente afeta seu bem-estar. Sendo assim este trabalho teve como objetivos avaliar os aspectos relacionados ao comportamento e bem-estar das aves, comparando o sistema de criação convencional (bateria de gaiolas) com um sistema de criação em cama, ninho e poleiro, bem como avaliar a influencia das condições ambientais (estresse e conforto) na qualidade final dos ovos, associando técnicas da zootecnia de precisão (análise de imagens), para avaliar as respostas comportamentais das diferentes linhagens nas diferentes condições. Dois grupos de 20 aves (10 Hy-line W36 e 10 Hy-line Brown) em inicio de postura foram submetidas a duas condições ambientais durante duas semanas consecutivas (26°C e 60% UR e 35°C e 70%UR), sendo também submetidas a dois sistemas de criação (bateria de gaiolas e cama). Durante o período de avaliação um sistema de câmeras de vídeo registrava o comportamento das aves. Os ovos produzidos durante todo o período da pesquisa foram avaliados pela análise dos seguintes parâmetros de qualidade: peso do ovo, espessura da casca, gravidade especifica, unidades Haugh, além de análise da colorimetria da gema, porosidade da casca e análises microbiológicas, para a verificação de uma possível ocorrência de contaminação por Salmonella sp na gema e casca dos ovos. Os resultados obtidos revelaram uma redução significativa (P<0,05) nos parâmetros de qualidade do ovo para a condição de estresse térmico, principalmente para o sistema de criação em gaiolas. Quanto as análises de comportamento, o sistema de criação em cama proporcionou a expressão de todos os comportamentos naturais e de conforto das aves, favorecendo assim melhores condições de bem-estar. Para o sistema de criação convencional (gaiolas) foi possível verificar que mesmo sem nenhuma condição, as aves ainda tentavam executar seus comportamentos naturais, sendo que a impossibilidade de expressarem estes comportamentos certamente agravou a condição de estresse provocada por este sistema de criação. Pela análise dos dois sistemas de criação e das duas condições ambientais a linhagem Hy-Line W36. foi a que obteve um melhor desempenho. / The battery cage system is a very polemic issue in European countries, being the most commonly raised concerns focused on the spatial restrictions of the hens, which might compromise important comfort movements affecting their welfare conditions. This work aimed to evaluate aspects related to behavior and welfare of hens comparing a conventional housing system (laying cages in battery) with another system using litter, lair and roost. In addition, the effects of environmental conditions (stress or comfort) on final egg quality and behavior responses (analyzed by precision techniques) were evaluated in different laying hen lines. Two groups of 20 birds (10 Hyline W36 and 10 Hy-line Brown) at the beginning of production phase were submitted to two environmental conditions (26°C with 60% RU or 35°C with 70% RU) and two housing systems (cages in battery or litter) during two consecutive weeks. During the evaluation period, bird behaviors were recorded by video cameras. Total egg production was analyzed according to egg weight, shell thickness, specific gravidity, Haugh unit, yolk colorimetric parameters and shell porosity. Eggs were microbiologically analyzed in order to detect Salmonella sp contamination in yolk and eggshell. A significant (P<0.05) reduction in quality parameters was observed in eggs from laying hens raised under heat stress, mainly in those from laying cage system. In relation to behavior, the housing system in litters proportioned the expression of all natural behaviors and those related to bird comfort, which permitted to improve welfare conditions. In conventional system (laying cages) was verified that birds tried to manifest their natural behavior in spite of lacking condition for it. This impossibility for executing natural behaviors promoted an increase of stress provoked by the housing system. Analyzing both systems and environmental conditions, hens from Hy-Line W36 showed the better performances.

Thesis (Ph.D.)--University of Aberdeen, 2005. / Includes bibliographical references. Available in PDF format via the World Wide Web.

Diss. (sammanfattning) Linköping : Univ., 2003. / Härtill 4 uppsatser.

Os fatores idade e sexo modificam a experiência dolorosa em animais e seres humanos. Os efeitos dos hormônios gonadais têm sido estudados em diversos modelos experimentais de dor, no entanto, o efeito do envelhecimento na percepção à dor carece de mais investigação. O efeito do envelhecimento na dor neuropática ainda não está bem estabelecido. Neste estudo se procurou avaliar possíveis variações na percepção da dor da hiperalgesia mecânica, em função da idade, presença e ausência de hormônios gonadais e sexo em ratos Wistar machos e fêmeas, jovens e idosos no modelo de dor neuropática, ligadura da quinta raiz lombar. Os animais foram divididos nos seguintes grupos: ratos jovens orquiectomizados e não-orquiectomizados, ratos idosos orquiectomizados e não-orquiectomizados, ratas jovens ooforectomizadas com ou sem reposição de 17beta-estradiol e ratas idosas. Foi testado o limiar de retirada da pata após estímulo mecânico antes da ligadura e no 7º, 14º, 21º e 28o dias após a ligadura. Os resultados mostraram que todos os animais apresentaram comportamento hiperalgésico após ligadura da quinta raiz lombar durante os 28 dias de observação. A hiperalgesia ocorreu independentemente do sexo do animal, da presença ou ausência de hormônios gonadais ou idade. Não houve diferença entre ratos jovens e idosos não-orquiectomizados (p = 0,420), entre ratos jovens e idosos orquiectomizados (p = 0,560). Entre os ratos idosos com e sem orquiectomia houve diferença no 14º (p = 0,038) e 28º (p = 0,002) dias. Ratas jovens ooforectomizadas sem reposição de 17beta-estradiol apresentaram menor hiperalgesia que ratas ooforectomizadas com reposição durante todo o período (p = 0,001). Não houve diferença entre ratos idosos orquiectomizados e ratas idosas (p = 0,09). Ratos jovens não-orquiectomizados apresentaram menor hiperalgesia mecânica que ratas jovens ooforectomizadas com reposição de 17beta-estradiol (p = 0,001), o mesmo não ocorreu entre machos e fêmeas jovens gonadectomizados sem reposição hormonal (p = 0,511). Ratas jovens ooforectomizadas sem reposição de 17beta-estradiol e ratas idosas mostraram diferença apenas no 7º dia (p = 0,002). Os resultados permitiram concluir que a reposição de 17beta-estradiol aumentou a hiperalgesia mecânica aos estímulos mecânicos após ligadura da quinta raiz lombar em ratas jovens ooforectomizadas, a hiperalgesia mecânica não diferiu entre os sexos em ratos jovens machos e fêmeas, a presença da gônada masculina diminuiu a hiperalgesia mecânica em ratos idosos e que houve diminuição da hiperalgesia mecânica em ratas idosas / Age and sex modify the pain experience in animals and humans. The effects of gonadal hormones have been studied in various experimental pain models, however, the effect of aging on pain perception needs further investigation. The effect of aging on neuropathic pain is not well established. In this study, we sought to determine how aging and gonadal hormones affect mechanical hyperalgesia using spinal nerve ligation as a neuropathic pain model in aged and young male and female Wistar rats. Animals were divided into seven groups: aged female, ovariectomized young females with 17beta-estradiol replacement, ovariectomized young females without 17beta-estradiol replacement, orchiectomized and non-orchiectomized aged and young males. Rats were tested for mechanical hyperalgesia in the plantar surface of the left hindpaw before nerve ligation and on days 7, 14, 21 and 28 after nerve ligation. All animals of all groups showed mechanical hyperalgesic behavior after spinal nerve ligation during entire period of 28 days. Hyperalgesia was independent of the sex of the animal, the presence or absence of gonadal hormones or age. There was no difference between non-orchiectomized aged and young males (p = 0.420), and between orchiectomized aged and young males (p = 0.560). There was difference between aged male rats with and without orchiectomy in days 14 (p = 0.038) and 28 (p = 0.002). Young ovariectomized female rats without 17beta-estradiol replacement had less hyperalgesia than young ovariectomized female rats with replacement (p = 0.001). There was no difference between aged orchiectomized male rats and old female rats (p = 0.09). Young non-orchiectomized male rats showed less mechanical hyperalgesia than young ovariectomized female rats with 17beta-estradiol replacement (p = 0.001), that did not occur between young orchiectomized males rats and young ovariectomized females rats without 17beta-estradiol replacement (p = 0.51). Young ovariectomized female rats without 17beta-estradiol replacement and old female rats showed differences only on day 7 (p = 0.002). These data suggest that estradiol presented a pronociceptive effect in young female rats in mechanical hyperalgesia in the neuropathic pain model, spinal nerve ligation, withdrawal threshold did not differ between sex, the presence of male gonadal hormones reduces mechanical hyperalgesia in old male rats and there is reduced mechanical hyperalgesia in aged female rats

O objetivo da presente pesquisa foi fornecer subsídios, visando ao controle de Diatraea saccharalis (Fabricius, 1794) em milho, com o parasitóide de ovos Trichogramma galloi Zucchi, 1988. Foram realizados estudos do parasitóide, incluindo seleção de linhagens, efeito de fatores abióticos (temperatura e alimento) sobre parâmetros biológicos e de parasitismo, bem como comportamento de postura de D. saccharalis em milho. A seleção de linhagens foi conduzida nas temperaturas de 25 e 30°C e o efeito da temperatura sobre a longevidade e a capacidade de parasitismo foi estudado em 15 condições térmicas (na faixa de 10 a 38°C). O efeito de quatro tipos de alimentos foi avaliado sobre a longevidade e a capacidade de parasitismo. O comportamento de postura de D. saccharalis foi observado em dois níveis de infestação (dois e dez casais por planta). Constatou-se que a linhagem G16080 foi a mais adequada para liberações visando ao controle de D. saccharalis, com base na capacidade de parasitismo e na duração do ciclo e por ter apresentado desempenho equivalente na faixa de 25 a 30°C. A maior capacida de de parasitismo de T. galloi linhagem G16080 ocorreu entre 20 e 28°C, embora ten ha havido parasitismo em todas as temperaturas na faixa de 10 a 38ºC. Não houve correlação entre a longevidade e parasitismo, havendo uma concentração deste parasitismo quando o inseto viveu menos. A presença do hospedeiro interferiu na longevidade de T. galloi linhagem G16080. Considerando-se o parasitismo, mel puro e a solução de pólen são os alimentos mais adequados para T. galloi linhagem G16080. Com ou sem o hospedeiro, mel + pólen foi o alimento que proporcionou a maior longevidade para T. galloi linhagem G16080. Independentemente do nível populacional de D. saccharalis, houve uma acentuada preferência pela região do colmo para postura, em relação às folhas de milho de 45 dias de idade, o que pode definir a amostragem e a forma de liberação do parasitóide. / The objective of this study was to provide information aiming to control Diatraea saccharalis (Fabricius, 1794) in corn with the egg parasitoid Trichogramma galloi Zucchi, 1988. Studies realized on the parasitoid included strain selection; effect of abiotic factors (temperature and food) on biological parameters and parasitism as well as oviposition behavior of D. saccharalis in corn. Strain selection was conducted at a temperature of 25 and 30°C and the effect of temper ature on longevity and parasitism capacity was studied at 15 temperature conditions (in the range of 10 to 38°C). The effect of four types of food sources was evaluated on longevity and parasitism capacity. The oviposition behavior of D. saccharalis was observed in two levels of infestation (two and ten couples per plant). It was verified that the strain G16080 was more suitable for release with the aim to control D. saccharalis based on the parasitism capacity and duration of the life cycle and having equivalent performance at a temperature range of 25 to 30°C. The highest rate of parasitism by the T. galloi strain G16080 occurred between 20 and 28°C, althoug h there was parasitism in all other temperature ranges of 10 to 38ºC. There was no correlation between longevity and parasitism, with concentration of this parasitism when the insect lived less. The presence of the host interfered with longevity of the T. galloi strain G16080. Considering parasitism, pure honey and the pollen solution are more suitable as food for T. galloi strain G16080. With or without the host, honey + pollen was the food that produced the highest longevity for T. galloi strain G16080. Irrespective of the population level of D. saccharalis, there was a clear preference for oviposition on the stem in relation to 45 day old corn leaves that could define sampling and the form of release of the parasitoid.