A reduction in voluntary feed intake is a major factor in the lost productivity of grazing lambs infected by gastrointestinal parasites yet the mechanisms involved are poorly understood. Potential pathways involved in parasite-induced feed intake depression were investigated in lambs with minimal previous exposure to parasites and artificially infected by the small intestinal parasite Trichostrongylus colubriformis. Six in vivo experiments were conducted on lambs housed in individual pens or metabolism crates with similar feeding and experimental procedures. In Experiment 1 (Chapter 4) the effect of T. colubriformis infection on short term feed intake in lambs and of some pharmacological agents on feed intake depression were investigated. Prior to and for the duration of infection, lambs were fed once per day and feed intake recorded at regular intervals over the day (8 h). Following the onset of feed intake depression in the infected group (9 weeks after commencing dosing), all animals were treated with an analgesic (codeine phosphate per os), an anti-inflammatory agent (indomethacin per os), a CCK antagonist (L364-718 by subcutaneous injection) or saline (control) in a replicated Latin square design (n = 8). Although the pattern of feed consumption was similar in infected and non-infected lambs, average daily intake was reduced 32 % and short term intake (recorded at 10 minute intervals for the first hour of feeding, 15 minute intervals for the second hour and hourly for the next 6 hours of feeding) reduced 40 % by infection. This identified the key component by which intake was depressed and enabled the use of a short term intake model and short duration of action compounds to identify the pathways involved in intake depression in this sequence of experiments. None of the pharmacological treatments increased intake in the infected group. These results suggest a reduction in the rate of consumption due to reduced hunger signals, rather than change of meal eating patterns, is the major cause of feed intake depression. Specific conclusions about the pathways investigated using the pharmacological agents could not be obtained. Experiment 2 (Chapter 5) was designed to investigate the roles of pain and osmolality on feed intake depression. Digesta samples collected prior to and during parasite infection and before and after feeding had similar osmolalities (240-260 mosmol/l) which indicated that feeding or infection had no effect on osmolality of digesta. Following the onset of feed intake depression in infected animals, all animals were treated in a Latin square design (n = 4) with no treatment, saline, local anaesthetic (xylocaine) or analgesic (codeine phosphate) solution 15 minutes before feeding, by slow injection into the duodenum. There was no effect of these treatments on food intake. In the second part of the experiment, hyperosmotic solutions (mannitol and NaCI) markedly depressed short term intake in non-infected animals, suggesting a role for osmoreceptors in intake regulation. However these effects were not blocked by local anaesthetic so the depressed intake may have resulted from generalised malaise rather than from specific osmoreceptor effects. In Experiment 3 (Chapter 6) the role of peripheral CCK on intake depression was examined by a dose-response study utilising the CCK antagonist, loxiglumide. Intravenous injection of 5, 10 or 20 mg/kg LW of loxiglumide to infected lambs 10-15 minutes before feeding (n = 6) had no effect on feed intake at any of the dose levels. In experiment 4 (Chapter 7) loxiglumide was infused intravenously for 10 minutes (30 mg/kg/h) before feeding and for the first 2 h (10 mg/kg/h) after feed was offered to minimise any effect of the rate of clearance of loxiglumide on the lack of feed intake response. As well, the rate of marker disappearance from the abomasum was recorded in both infected and non-infected animals. Continuous infusion of loxiglumide did not attenuate parasite induced intake depression nor did it have any effect on abomasal emptying. Abomasal volume was reduced by infection (66.3 vs 162 ml) as was the fractional outflow rate (2.2 vs 2.8 ml/min) but these differences were accounted for by the lower level of feed intake in the infected animals. In Experiment 5 (Chapter 8) brotizolam, a benzodiazepine appetite stimulant, thought to act on the hypothalamus, was administered in a dose-response study to infected and non-infected animals (n = 4) immediately prior to feeding or following termination of the first meal (45 minutes after feeding) and the feed intake response recorded. Brotizolam elevated both the short term (0-0.75 h), daily (22 h) intake and all time intervals in the first 5 h after feeding in infected and non-infected animals when administered after the first meal but when administered prior to feeding elevated intake only over the first 6 h of feeding. In both cases the magnitude of the response was greater in infected animals than in non-infected animals. Brotizolam appeared to increase the rate of eating without having a major impact on meal eating patterns when administered before feeding. Where administration was after the first meal, the effect was due to an "extra" meal being consumed. These findings showed that infected animals can respond to central stimulators of intake although the mechanism of the response is not known. Opioids were implicated in intake depression as the rate of intake rather than meal patterns appeared to be the major parameter depressed under parasitism. This was examined in experiment 6 (Chapter 9) where animals (n = 6) were fasted for 26 h or not fasted, then treated with saline (control), brotizolam (intake stimulant) or naloxone (opioid antagonist) immediately prior to feeding. Fasting stimulated feed intake in the short term (100 % increase in 75 min) and over the day (12 % increase) in both infected and non-infected animals. Following fasting, infected animals ate a similar amount of feed to the non-infected, fasted animals and more than the non-infected, non-fasted animals. The signals resulting from a one day fast were sufficient in the short term to override parasite induced mechanisms causing feed intake depression. Naloxone suppressed the intake stimulatory effects of a 26 h fast in both infected and non-infected animals, which supports a role for endogenous opioids as hunger signals. Where animals were not fasted, naloxone reduced intake only in the non-infected animals which suggested endogenous opioid levels may be lower in infected animals than in non-infected animals. In the final experiment (Experiment 7, Chapter 10) the role of central hunger and satiety mechanism were investigated. Infected and non-infected animals (n = 6) were treated with naloxone or saline by intravenous injection, or saline and met-enkephalinamide (an opioid analogue) by intracerebral infusion, or naloxone and the opioid analogue simultaneously to investigate the role of central opioids in feed intake depression. To determine the role of CCK induced satiety signals on feed intake at a central level, loxiglumide and CCK were infused separately and in combination for 30 minutes prior to feeding and for the first 60 minutes of feed on offer, into a lateral cerebral ventricle of the brain of infected and control animals (n = 6). The opioid analogue tended to increase intake in infected animals but the effect was not significant probably because the dose used was too low to elicit a response in sheep. Naloxone depressed intake only in the infected animals, which conflicted with the results of Experiment 4. As a consequence these results were inconclusive because of the single low dose of opioid analogue used and the conflicting naloxone responses. CCK alone depressed intake by 39-52 % only in infected animals and this effect of the 90 minute infusion was evident over the 8 h short term recording period. Loxiglumide attenuated the feed intake depressive effects of CCK in the infected animals to the extent that intake was elevated above control levels. Loxiglumide alone was an intake stimulant in both infected and non-infected animals. Intake was increased over the entire 8 h but mostly in the second hour when intake was increased by 188 % in infected animals and by 16 % in the non-infected animals and resulted in almost continuous eating. These results showed loxiglumide will temporarily block the effect of parasite infection on feed intake in sheep when administered centrally and the fact that it blocked the effects of exogenous CCK on intake indicated that the effect is mediated via CCK receptors. In conclusion GIT parasite infection reduced both short term and daily feed intake apparently by a change in rate of intake rather than any alteration in meal patterns. It was further suggested that anyone of a number of potential peripheral pathways, including changes to osmolality, gut emptying, pain and inflammation of the gut, alone is not involved in anorexia in sofar as the compounds used could block these factors and the results support the idea that intake depression is mediated via a central mechanism. Intake in infected animals responded to a much greater extent when fasting, i.c.v. loxiglumide or brotizolam were employed. Feed intake thus appears to be regulated through the same mechanisms in infected and non-infected animals. The results from compounds affecting the central mechanism suggest central CCK receptors are important in parasite induced anorexia, possibly by changing the onset of satiety or by interacting with endogenous opioids to reduce the rate of feed intake. Secondly reduced endogenous opioids may be causing the reduction in the rate of feed consumption alone or as a result of other interactions. It was concluded that intake in parasitised animals could be increased to that of control animals by employing procedures and compounds thought to act on the hypothalamus.

Submitted by Aelson Maciera (aelsoncm@terra.com.br) on 2017-06-14T18:45:04Z No. of bitstreams: 1 DissGAO.pdf: 2694531 bytes, checksum: 5a6ae41e5754d53d9fb2b7a3bc00ea15 (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-06-26T19:26:58Z (GMT) No. of bitstreams: 1 DissGAO.pdf: 2694531 bytes, checksum: 5a6ae41e5754d53d9fb2b7a3bc00ea15 (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-06-26T19:27:05Z (GMT) No. of bitstreams: 1 DissGAO.pdf: 2694531 bytes, checksum: 5a6ae41e5754d53d9fb2b7a3bc00ea15 (MD5) / Made available in DSpace on 2017-06-26T20:13:29Z (GMT). No. of bitstreams: 1 DissGAO.pdf: 2694531 bytes, checksum: 5a6ae41e5754d53d9fb2b7a3bc00ea15 (MD5) Previous issue date: 2012-10-30 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / As an ethnography about interspecific relationships – between humans and animals – in urban contexts, this dissertation aims to reflect about the actions related to the management and protection of animal life within institutional and domestic scopes. From the contact with activist groups in two cities in the state of São Paulo it was possible to observe situations that alternate between containment and propitiation measures and their consequent interferences in a conviviality supported by the principle of animal protection. Government and activism – as well as the perceptions and conceptions of the domestic animal resulting from their respective actions – allow a theoretical and methodological reflection on the binomial established between culture and nature, when they reveal conflicts and possible ambivalences of animal protection, situated between control and hosting measures that, consequently, are linked to affectivities and legal issues. Ethical arguments, contained in laws and public policies, as well as the appeal to the sensitive dimension, eventually triggered by activism, also provide subsidies to understanding other concepts - such as welfare and animal rights – and these interspecific relationships mediated by emotions and reason, both tangents to the moral condition of the animal as part of a predominantly human socius. / Como uma etnografia acerca de relações interespecíficas – entre humanos e animais – em contextos urbanos, este trabalho visa refletir sobre as ações envolvidas na gestão e tutela da vida animal no âmbito institucional e doméstico. A partir do contato com grupos ativistas em dois municípios paulistas, foram observadas situações que se alternam entre medidas de contenção e de propiciação e suas consequentes interferências num convívio amparado pelo princípio da proteção aos animais. Tanto o poder público como o ativismo – bem como as percepções e concepções sobre o animal doméstico resultantes de suas respectivas ações – possibilitam uma reflexão de ordem teórico-metodológica do binômio estabelecido entre cultura e natureza, ao apresentarem os conflitos e as possíveis ambivalências da proteção animal, situados entre medidas de controle e de acolhimento e, por conseguinte, imbricados a afetividades e juridicidades. Argumentos éticos, presentes em legislações e em políticas públicas, e o apelo à dimensão do sensível, eventualmente acionado pelas atividades militantes, também fornecem subsídios à compreensão de outros conceitos – como bem-estar animal e direitos animais – e dessas relações interespecíficas mediadas por emoções e razões, ambas tangentes à condição moral do animal enquanto parte de um socius predominantemente humano.

<p>Bovine respiratory disease (BRD) is an ongoing health and economic issue in the dairy and beef cattle industry. Also, there are multiple risk factors that make an animal susceptible to BRD and it's diagnosis and treatment is a challenge for producers. Four bacterial species, <em>Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, </em>and<em> Mycoplasma bovis</em> have been associated with BRD mortalities. Hence, this study aims to characterize the cattle nasal microbiome as a potential additional diagnostic method to identify animals suspected to have a lung infection. Quantitative PCR and 16S rRNA gene sequencing were used to determine the bacterial load of these four bacterial pathogens in the nasal microbiome of apparently healthy (N=75) and (N=58) affected by BRD Holstein steers. We then sought to identify a value or equation that could be used to discriminate between BRD and healthy animals using a Linear Discriminant Model (LDA). Additionally, co-occurrence between commensal bacterial and BRD-pathogens were also identified. Cattle diagnosed with BRD presented lower richness, evenness and phylogenetic diversity than healthy pen-mates. Bacterial species and genera <em>Truperella pyrogenes </em>and <em>Bibersteina</em> were increased in the BRD group, and the species <em>Mycoplasma bovirhinis</em> and <em>Clostridium sensu stricto</em> increased in the healthy group. Prevalence of <em>H. somni </em>(98%)<em> </em>and <em>P. multocida </em>(97%) were the highest regardless of disease diagnosis in all the samples. Prevalence of <em>M. haemolytica </em>(81 vs. 61%) and<em> M. bovis </em>(74 vs. 50.7%) were higher in the BRD group. The bacterial density of <em>M. haemolytica</em> and<em> M. bovis </em>was also higher in the BRD group, whereas <em>Histophilus somni</em> was lower in the BRD group. Five different models were tested using LDA, and one model produced a sensitivity and specificity of 60% and 81% agreement with diagnosis based on animal symptoms. Co-occurrence analysis demonstrated that the nasal microbiome members are more likely to interact with each other than associations between BRD-pathogens and nasal microbiome members. This study offers insight into the BRD-pathogens prevalence and difference in nasal microbiome between healthy and BRD animals and provides a potential platform for future studies and potential pen-side diagnostic testing.</p>

Humane education (HE) is a specialized niche in higher education and adult learning. HE provides a curricular framework positioning environmental ethics, animal protection, human rights, media literacy, culture, and change processes as the nexus for understanding and inspiring social change. Research-derived experiences illuminating how educators conceptualize and implement HE in U.S. schools are absent from the scholarly literature. Facing this gap, practitioners and administrators of HE programs cannot access nor apply research-derived practices to inform instruction. To address this gap, a conceptual framework was advanced weaving together HE teaching experience, Freirean philosophy, hyphenated selves, reflection-in-action, transformative learning, and transformative education to explore and understand what it means to be a practitioner teaching through the lens of HE in U.S. primary, secondary, and post secondary classrooms. A qualitative, multi case study was designed wherein purposeful and maximum variation sampling resulted in the recruitment of 9 practitioners working in Kindergarten to post secondary contexts. Eight practitioners were alumni of HE programming, and 1 practitioner engaged self-study of HE pedagogy. Each bounded system included the HE practitioner, his or her classroom context, and local school community. Interviews, document review, within-case analysis, and cross-case analysis resulted in key themes illuminating the need to design a comprehensive system of field-based learning and ongoing professional support to benefit HE practitioners. A policy recommendation is provided to shape programming, policy development, and resource allocation to improve and sustain HE as a field of study and professional practice.

<p>Thyroid hormone is essential for regulating adult metabolism and proper fetal development. Under normal conditions, maternal and fetal thyroid hormones are subject to metabolism at the placenta and within fetal tissues through deiodination and sulfation to regulate fetal exposure to the bioactive hormone. Disruptions of the thyroid hormone system can result in non-thyroidal illness syndrome (NTIS), which is classified as the dysregulation of thyroid hormone homeostasis. The exact cause of the alterations in circulating thyroid hormone levels during NTIS is not well- known. In comparison, hypothyroidism results from the absence of thyroid hormone production and presents as low thyroid hormone levels.</p> <p>Porcine reproductive and respiratory syndrome virus (PRRSV) crosses the late gestation placenta and causes suppression of circulating maternal and fetal thyroid hormone. Chapter 2 investigates the potential role of thyroid hormone metabolism in this disruption. Pregnant gilts were challenged with PRRSV2 (n=22) or sham inoculated (n=5) at gestation day 85. Samples were collected on day 106, and viral load was assessed in fetal serum and thymus. From the entire fetal population, three distinct subsets of fetuses representing biological extremes were identified, including uninfected with no detectable viral load (UNIF), high viral load viable (HV-VIA), or high viral load with severe meconium staining (HV-MEC). In addition, control fetuses from sham inoculated gilts (CON) were used as a reference group. Samples of fetal liver, kidney, and the corresponding fetal placenta and maternal endometrium for n=10 fetuses per group were then used to evaluate gene expression. A total of 11 genes associated with thyroid hormone metabolism including deiodinases (DIO1,2,3), sulfotransferases (SULT1A3,1B1,1C2,1E1,2A1), sulfatase (STS), and solute carriers (SLC16A2,16A10) were quantified using absolute quantification qPCR. Evidence of fetal decompensation was observed within the high viral fetuses in the form of decreased DIO1 expression within the fetal liver and increased DIO3 expression in both components of the placenta. Circulating levels of T4 and inactive thyroid hormone metabolites, reverse-triiodothyronine (rT3) and two diiodothyronines (3,5-T2 and 3,3’-T2), were measured in fetal serum. While T4 was depressed, no change was observed in circulating rT3 levels, and neither T2 metabolite reached the lower detection limit. This may suggest that alterations in thyroid hormone metabolism generate a localized effect on hormone metabolites in the respective tissues.</p> <p>Alternatively, the low levels of available T3 and T4 limit the production of downstream metabolites to be found in serum.</p> <p>The cause-and-effect relationship between PRRSV infection, fetal thyroid disruption, and the effects on fetal thyroid hormone metabolism are unclear. Therefore, Chapter 3 developed a non-pathogenic model using methimazole (MMI) to induce hypothyroidism in the late gestation fetus and evaluate the impact on fetal development and thyroid hormone metabolism. Pregnant gilts were either treated with oral methimazole or equivalent sham from gestation day 85-106 (n=4/group), followed by classification of all fetuses as live, live but meconium stained, or dead. Fetuses exposed to MMI in-utero were notably hypothyroid with significantly suppressed serum T3 and T4 and histological evidence of goiter. Surprisingly, fetuses from MMI-treated dams were substantially larger but appeared to exhibit non-allometric growth with an increase in girth but not length. The liver, kidney, and the corresponding fetal placenta and maternal endometrium were collected from a subset of 16 fetuses per group to evaluate the relative expression of five genes associated with thyroid hormone metabolism, including three deiodinases and two solute carriers known to transport thyroid hormone. Compensatory transcription of DIO3 was observed in all tissues evaluated, suggesting increased vertical transfer of maternal thyroid hormone at the placenta and decreased breakdown of thyroid hormone within fetal organs.</p> <p>The evaluation of thyroid hormone metabolism within the fetus and within the placenta has allowed us to differentiate suppressed thyroid hormone levels of the pig fetus under pathogenic and non-pathogenic conditions. In the context of PRRSV infection, the observed decompensation of thyroid hormone metabolism would further exacerbate the hypothyroid state and is therefore consistent with NTIS. In contrast, fetuses with induced thyroid hormone suppression following maternal exposure to MMI showed compensatory thyroid hormone metabolism in the same tissues. This indicates true hypothyroidism and clearly demonstrates a fetal capacity to respond to such endocrine disruption.</p>

非營利組織之事業企劃書：以台灣動物平權促進會為例 / With the high level of economic development, there are more and more social issues caused and some nonprofit organizations have founded for advocating core values to influence people and achieve social changes. However, high organizational performance and good organizational effectiveness are not universal in nonprofit organizations; therefore, nonprofit organizations are recommended to refer to marketing knowledge for increasing their organizational effectiveness. This business plan is using a Taiwan nonprofit organization as a case to study how to transfer commercial marketing knowledge into nonprofit organizations for contributing organizational success.

Um componente considerado crítico para o manejo bem sucedido de populações silvestres é a manutenção da variação genética. No intuito de avaliar a magnitude e a distribuição da variabilidade genética existente em populações de Caiman latirostris, nove populações distribuídas ao longo do eixo latitudinal da distribuição da espécie no Brasil e uma população em cativeiro foram estudadas com auxílio de onze locos microssatélites. A diversidade gênica média (He=h) e a heterozigosidade média observada (Ho) apresentaram valores elevados, 0,628 e 0,567, respectivamente, denotando existência de elevada variabilidade genética para esta espécie nas regiões de estudo. A população paulista mesmo estando na zona intermediária de distribuição geográfica no Brasil não possui a maior variabilidade genética. O valor FST estimado foi 0,270 e o RST foi 0,342. Ambas as medidas de diferenciação entre as populações foram significativas (P<0,05). As altas estimativas de FST e RST sugerem a ausência ou fluxo gênico restrito entre essas populações, exceção feita entre as populações de Natal (RN) e João Pessoa (PB), onde não houve diferenciação significativa entre as populações, sugerindo assim que há fluxo gênico entre elas, fato confirmado pelo coeficiente de parentesco. Pôde-se concluir neste trabalho que a distância genética entre a população do litoral é afetada pela existência da Serra do Mar como barreira geográfica pelo fato desta população apresentar as maiores diferenciações genéticas e não se agrupar a nenhuma população pelo método de agrupamento UPGMA, mesmo estando próxima das populações paulista. A distância genética entre as populações parece não acompanhar a distância geográfica, em termos de gradiente latitudinal (r=0,206). Entretanto quando retiramos a população da Ilha do Cardoso esta correlação aumenta significativamente (r=0,540), indicando haver um certo padrão espacial da variabilidade genética entre as populações. O coeficiente médio de parentesco foi baixo entre e dentro das populações estudadas. Com estes resultados podemos começar a entender a dinâmica e estrutura social de populações de Caiman latirostris, e quanto mais se compreende sobre a biologia destes animais mais precisa serão decisões visando condições que permitam a existência continua da espécie. / A component considered critical for the managment well succeed of wild populations it is the maintenance of genetic variation. In the intention of evaluating the extend and the distribution of the existent genetic variability in populations of Caiman latirostris, nine populations distributed along the latitudinal axis of the distribution of the species in Brazil and a population in captivity they were studied with aid of eleven locos microsatellite. The genic mean diversity (He=h) and the observed mean heterozygosity (Ho) across all loci for all populations ranged from 0,628 and 0,567, respectively, denoting existence high genetic variability. The population from São Paulo being in the intermediate zone of geographical distribution in Brazil doesn't possess the largest genetic variability. The value dear FST was 0,270 and RST it was 0,342. Both differentiation measures among the populations were significant (P < 0,05). The higher estimates of FST and RST suggested a absence or low gene flow among those populations, exception done between the populations of Natal (RN) and João Pessoa (PB), where there was not significant differentiation among the populations, suggesting a gene flow pattern among them, fact confirmed by the related coefficient. It could be concluded in this work that the genetic distance among the population of the coast is affected by the existence of the Mountain of the Sea as geographical barrier for the fact of this population to present the largest genetic differentiations and not to group the any population for the grouping method UPGMA, same being close of the populations from São Paulo. The genetic distance among the populations seems not to accompany the geographical distance, in terms of latitudinal gradient (r=0,206). However when we removed the population of Cardoso's Island this correlation it increases significantly (r= 0,540), indicating there to be a certain space pattern of the genetic variability among the populations. The medium coefficient of related was low among and inside of the studied populations. With these results we can begin to understand the dynamics and it structures social of populations of Caiman latirostris.

殘酷時尚議題已成為當代的芒刺，對動物保護團體及人權組織形成挑戰。善待動物組織(People for the Ethical Treatment of Animals：PETA)是目前規模最大、發展最完善的動物權利組織，其廣告往往能在人們心中喚醒善待動物的意識。PETA首次在時尚雜誌上發起的反對毛皮廣告活動，即獲得「非營利組織最佳廣告獎(Best Ads Produced by a Nonprofit)」。 本研究的宗旨為，展示公益內容廣告運動可在任何市場導向的經濟體中運作。本研究列舉PETA的PSA廣告範例如下：「我寧願裸體」（“I’d rather go naked”, 1992年) 、「毛皮代表死亡」(“Fur is dead”, 2001年)、「對皮膚感到自在」(“Be comfortable in your own skin”, 2003年)、「拯救海豹」(“Save the seals”, 2009年)。「毛皮」 （“Skins”）主題上述廣告皆由知名女性代言。 本研究為質量研究，採用射影技術(projective technique)及深入訪談，調查結合訊息與圖像的PSA廣告在傳達動物權力相關議題時的效果。本研究將指出這些廣告可如何透過1.有創意、逼真的圖像、2.說服力的寓意訊息及3.知名代言人，加強傳遞人與自然關係的概念。 本研究發現，具說服力的訊息必須擁有逼真、富含寓意、簡潔、清楚等特性，且僅有在搭配適當的圖像時，才能發揮效力。此類圖像必須擁有不含混、非情色誘因、有創意、外來風或暗中使用性訴求等特性。廣告必須同時訴諸感性訴求及現實主義，並由知名、受人敬重的代言人出演。當公益廣告意圖以幽默感營造有利的風趣情境時，就不應使用嚴肅形象，或要求觀眾訴諸行動。至於訴諸恐懼類的公益廣告，則結合令人反感的圖像特徵及寓意訊息，仍可營造有利觀眾回應的情境，然而太過強烈的反感特徵有時會造成觀眾無法掌握寓意，導致觀眾無法回應。訴諸恐懼類平面廣告所引起的觀眾回應差異，以及本研究的局限性，可歸因於應答者的文化差異。 本研究做出結論為，擁有優良公眾形象及正面背景的知名代言人，可成功向大眾傳遞寓意訊息。再者，有創意、逼真，但不過度暴力、過度情色的圖像可吸引觀眾目光，促使大眾意識並做出反應。 / In the Modern Age the problem of killing fashion has become a thorny issue for the fashion industry, animal rights protection organizations, animal world and humanity. In this respect, People for the Ethical Treatment of Animals (PETA) performs as the largest and most highly developed animal rights institution in modern countries. PETA’s advertising has made a revolution in people’s minds concerning the ethical treatment of animals and already the first advertising campaign by PETA received the “Best Ads Produced by a Nonprofit” Award for its anti-fur advertising campaign in fashion magazines. The purpose of the study is to show how such social content advertising campaigns can work in all countries that are learning to live under market-driven economies. This research concerns properly selected print PSA samples of “I’d rather go naked” (launched in 1992), “Fur is dead” (launched in 2001), “Be comfortable in your own skin” (launched in 2003), and “Save the seals” (launched in 2009) campaigns presented by a number of celebrity spokeswomen posing for PETA’s “Skins” print advertisements. Based on qualitative research with the use of projective techniques and in-depth interviews, the study investigates the impact of a combination of PSA specific messages and image characteristics concerning animal rights using Public Service Print Advertising. The research will show how these ads hopefully can improve their effectiveness in order to achieve more respect for the human-nature relationship by using 1) Creative, realistic images, 2) A powerful educational message, and 3) A highly respected and appreciated celebrity spokesperson. The study found that a strong persuasive message is defined as realistic, educational, laconic, clear and the advertisement is ultimately effective only when used with proper image characteristics. Such image characteristics are non-vulgar, non-sexually distracting, but creative, exotic, or latent in sexual appeal. An advertisement should use emotional appeal and realism, presented by a celebrity spokesperson with a respected personal background. While PSA uses a high degree of humor to produce favorable and amused attitude, they do not present a serious image or promote a call to action. Regarding using fear appeal, strongly unpleasant image characteristics combined with educational messages still may produce a favorable and responsible attitude. However, it was found that unfavorable perceptions caused by strongly unpleasant images sometimes distracted viewers from the educational message or failed to produce a responsible reaction. The striking differences among public responses regarding fear appeal in print ads and study limitations can be attributed to cultural differences within respondents. The study has concluded that strong educational messages spoken by highly trustworthy celebrities with positive background activity makes the public listen. Furthermore, creative, realistic, but not overly-violent or overly-sexual images catch the viewer’s attention, makes the public more aware and encourages responsible thinking.

Um componente considerado crítico para o manejo bem sucedido de populações silvestres é a manutenção da variação genética. No intuito de avaliar a magnitude e a distribuição da variabilidade genética existente em populações de Caiman latirostris, nove populações distribuídas ao longo do eixo latitudinal da distribuição da espécie no Brasil e uma população em cativeiro foram estudadas com auxílio de onze locos microssatélites. A diversidade gênica média (He=h) e a heterozigosidade média observada (Ho) apresentaram valores elevados, 0,628 e 0,567, respectivamente, denotando existência de elevada variabilidade genética para esta espécie nas regiões de estudo. A população paulista mesmo estando na zona intermediária de distribuição geográfica no Brasil não possui a maior variabilidade genética. O valor FST estimado foi 0,270 e o RST foi 0,342. Ambas as medidas de diferenciação entre as populações foram significativas (P<0,05). As altas estimativas de FST e RST sugerem a ausência ou fluxo gênico restrito entre essas populações, exceção feita entre as populações de Natal (RN) e João Pessoa (PB), onde não houve diferenciação significativa entre as populações, sugerindo assim que há fluxo gênico entre elas, fato confirmado pelo coeficiente de parentesco. Pôde-se concluir neste trabalho que a distância genética entre a população do litoral é afetada pela existência da Serra do Mar como barreira geográfica pelo fato desta população apresentar as maiores diferenciações genéticas e não se agrupar a nenhuma população pelo método de agrupamento UPGMA, mesmo estando próxima das populações paulista. A distância genética entre as populações parece não acompanhar a distância geográfica, em termos de gradiente latitudinal (r=0,206). Entretanto quando retiramos a população da Ilha do Cardoso esta correlação aumenta significativamente (r=0,540), indicando haver um certo padrão espacial da variabilidade genética entre as populações. O coeficiente médio de parentesco foi baixo entre e dentro das populações estudadas. Com estes resultados podemos começar a entender a dinâmica e estrutura social de populações de Caiman latirostris, e quanto mais se compreende sobre a biologia destes animais mais precisa serão decisões visando condições que permitam a existência continua da espécie. / A component considered critical for the managment well succeed of wild populations it is the maintenance of genetic variation. In the intention of evaluating the extend and the distribution of the existent genetic variability in populations of Caiman latirostris, nine populations distributed along the latitudinal axis of the distribution of the species in Brazil and a population in captivity they were studied with aid of eleven locos microsatellite. The genic mean diversity (He=h) and the observed mean heterozygosity (Ho) across all loci for all populations ranged from 0,628 and 0,567, respectively, denoting existence high genetic variability. The population from São Paulo being in the intermediate zone of geographical distribution in Brazil doesn't possess the largest genetic variability. The value dear FST was 0,270 and RST it was 0,342. Both differentiation measures among the populations were significant (P < 0,05). The higher estimates of FST and RST suggested a absence or low gene flow among those populations, exception done between the populations of Natal (RN) and João Pessoa (PB), where there was not significant differentiation among the populations, suggesting a gene flow pattern among them, fact confirmed by the related coefficient. It could be concluded in this work that the genetic distance among the population of the coast is affected by the existence of the Mountain of the Sea as geographical barrier for the fact of this population to present the largest genetic differentiations and not to group the any population for the grouping method UPGMA, same being close of the populations from São Paulo. The genetic distance among the populations seems not to accompany the geographical distance, in terms of latitudinal gradient (r=0,206). However when we removed the population of Cardoso's Island this correlation it increases significantly (r= 0,540), indicating there to be a certain space pattern of the genetic variability among the populations. The medium coefficient of related was low among and inside of the studied populations. With these results we can begin to understand the dynamics and it structures social of populations of Caiman latirostris.

This Diploma Thesis focuses on the issue of reproduction of animals and the non- profit organisations and subjects working in this field. The cat and dog reproduction is a primary cause of their overpopulation in the Czech Republic which becomes an ever- increasing issue as the abandoned animal shelter network fails to provide enough capacity for local demand. The thesis is focused on the activities of such organisations and tools they use for mobilising the necessary resources as well as the issue of animal reproduction itself. The theory basis is in the general theory of resource mobilisation supported with the explanation of the civil initiatives dedicated to this phenomenon as well as the overview of the phenomenon of the animal reproduction as well. The thesis is composed of five qualitative interviews with organisations or movements that are dedicated to effective reduction of animal reproduction. The empiric part of the thesis presents selected organisations and then analyses the collected data. The financial and human resources are mostly utilised by organisations which physically treat animals and the majority of the funds is consumed by providing care for them. On the other hand, organisations dedicated to enlightenment and education are able to function as self-funded and self-organised...