Septinas constituem uma família de proteínas de ligação a GTP que foram inicialmente identificadas em levedura Saccharomyces cerevisiae, mas também estão presentes em outros eucariotos com exceção de plantas. Septinas são purificadas de leveduras, Drosophila e cérebros de mamíferos na forma de filamentos, porém o mecanismo através do qual acorre a formação destes filamentos ainda não é muito bem compreendido. Septinas são constituídas de três regiões principais: um N-terminal variável, um domínio central GTPase altamente conservado e um domínio coiled-coil C-terminal. O gene SEPT4 foi identificado por M. Tanaka e colaboradores a partir do cDNA de cérebro humano e apresentou duas distintas transcrições: Bradeiona ? e ?. Interessantemente, além de cérebro e coração, as proteínas Bradeiona Α e Β. são detectadas somente em câncer do cólon, reto, próstata e melanoma maligno. Neste trabalho, o gene da proteína Bradeiona Β foi subclonado em um vetor de expressão bacteriano, produzido em E. coli e purificado com sucesso. O espectro de dicroísmo circular (CD) mostrou o perfil característico de proteínas com hélices a na estrutura secundária. Resultados de cromatografia de exclusão molecular (SEC) e espalhamento dinâmico de luz (DLS) indicam que a septina Bradeina foi produzida na forma de um estável oligômero com características monodispersivas, que foi subseqüentemente cristalizado em PEG6000. A atividade GTPase da Bradeiona Β foi comprovada através da técnica de eletroforese capilar (CE), mostrando-se absolutamente dependente de íons Mg2+. Inibição da atividade GTPase foi verificada em altas concentrações de Mg2+ (maiores que 5 mM). Com a finalidade de caracterizar os domínios preditos da Bradeiona Β (Fragmento Conservado e domínio GTPase), essas regiões foram previamente definidas, expressas em E. cozi e purificadas com sucesso. Resultados de CD, SEC, espectroscopia de fluorescência e NMR-600MHz indicam que o FC foi produzido na forma de um estável monômero com pouca estrutura secundária regular. Resultados de DLS e CD indicam que a fusão 6xHis-DGTPase foi produzida na forma de um oligômero com a presença de hélices a na estrutura secundária. A fusão 6xHis-DGTPase mostrou-se instável a altas concentrações na ausência de imidazol. A atividade GTPase da fusão GST+DGTPase foi comprovada, similarmente a Bradeiona , através da técnica de CE. Novamente, verificou-se dependência de íons Mg2+ (para a atividade catalítica) e inibição em altas concentrações de Mg2+. A fusão GST+DGTPase também foi capaz de hidrolisar ATP. Espera-se que as informações relatadas neste estudo proporcionem um alicerce para estudos estruturais/funcionais futuros das proteínas Bradeiona Α e Βoutras septinas. / Septins form a class of eukaryoyic guanine nucleotide-binding proteins that were first identified in budding yeast. Septins purified from yeast, Drosophila and mammalian brain form filaments, however the mechanism by which the filaments assemble is unclear. Septins have a highly conserved structure, which includes a central GTP-binding domain, a variable N-terminal region, and most septins also contain a coiled coil domain at the Cterminus. Bradeion p is one of the splice variants of the human septin gene, SEPT4, recently isolated by expression screening of an adult human brain cDNA library. The Bradeion gene resides at 17q23, and has been shown to present specific expression in both human colorectal cancer, urologic cancers and malignant melanoma. In order to characterize the GTPase activity of Bradeion Β , the protein was successfully expressed in E. coli and purified. The recombinant protein was characterized by circular dichroism (CD), dynamic light scattering (DLS) and a novel non-radioactive enzyme assay, which utilizes capillary electrophoresis (EC) to monitor GTP hydrolysis. The CD spectrum exhibited the typical shape characteristic of the presence of helical elements of secondary structure and the DLS pattern was indicative of a monodisperse solution, which was readily crystallized in the presence of PEG6000. GTP hydrolysis was shown to be Mg2+ dependent within the low millimolar range but at 5 mM was inhibitory. In order to characterize the predicted domains of Bradeion Β, these defined regions were successfully expressed in E. cozi and purified. The CD spectrum of CF exhibited the shape typically found for non-regular structure. The results of fluorescence spectroscopy, gel filtration (SEC) and NMR-600MHz also corroborate with the CD results indicating an irregular structure. The fusion protein 6xHis-DGTPase exhibited a CD spectrum with the typical shape characteristic of the presence of helical elements but was show to be instable at high concentrations in the absence of imidazole. To characterize the GTPase activity of the fusion protein GST+DGTPase, the CE technique was used to monitor GTP hydrolysis. Analysis by CE showed that GST+DGTPase was functional, since both GTP and ATP hydrolysis was observed in a Mg2+ dependent manner. This work provides novel approaches, which should aid in the fbture study of the structure and fùnction of Bradeion Α e Β, others septins and related GTPases.

The development and application of unnatural amino alcohols,prepared via hetero-Diels-Alder reactions,in synthesis and catalysis is described.The studies are concerned with the [i]scope of the hetero-Diels-Alder reaction and preparation of important intermediates in the synthesis of antiviral agents,[ii ]application of amino alcohols in the ruthenium transfer hydrogenation of ketones,[iii ]use of similar precursors in the in situ generation of oxazaborolidines for reduction of ketones,and [iv] development and application of new chiral auxiliaries for dialkylzinc additions to activated imines, respectively. [i ]The use of chiral exo -2-azanorbornyl-3-carboxylates in the preparation of enantiopure cyclopentyl-amines is described.At the same time the scope of the hetero-Diels-Alder reaction,used in their preparation,is extended by manipulations of the dienophiles. [ii ]Application of 2-azanorbornyl-3-methanol as a very efficient ligand in the ruthenium-catalysed asymmetric transfer hydrogenation of aromatic ketones.This ligand (2 mol%)in combination with [RuCl2(p -cymene)]2 (0.25 mol%)gave rise to a very fast reaction (1.5 h)leading to the reduced products in excellent yields and enantioselectivities (up to 97%ee ). [iii ]Preparation of α-disubstituded 2-azanorbornyl-3-methanols,in situ generation of the corresponding oxazaborolidines,and use of the latter in reduction of aromatic ketones.Concentration, solvent,and temperature effects on the reaction outcome are described. [iv ]Development of two generations of chiral auxiliaries for the addition of dialkylzinc reagents to N - (diphenylphosphinoyl)imines.Studies using density functional computations allowed the rationalisation of the reaction mechanism and the development of a second generation of ligands that improved the previously reported results.Up to 98%ee could be obtained with these new ligands. Solvent effects on the outcome of the reaction and extension of the work to a larger variety of N - (diphenylphosphinoyl)imines are described.

Recently, wireless access to Internet applications and services has attracted a lot of attention. However, there is no single wireless network that can meet all mobile users’ requirements. Con-sequently, integrated heterogeneous wireless networks are introduced to meet diverse wireless Internet applications and services requirements. On the other hand, integrated heterogeneous wireless networks pose new challenges to the design and development of reliable transport mechanisms. Wireless Application Protocol version 2 (WAP 2.0) is one of the promising trans-port mechanisms. It uses wireless profiled TCP (WP-TCP), which is fully compatible with TCP, as one of the reliable transport protocols to cope with the wireless link impairments. For WAP 2.0 to continue providing reliable and efficient transport services in the future, one of the key is-sues is to thoroughly study, understand, and improve its performance in integrated heterogeneous wireless networks. In this thesis, we develop analytical frameworks and propose a solution to respectively study and improve the performance of WP-TCP in integrated heterogeneous wireless networks. Spe-cifically, we consider WP-TCP short- and long-lived flows over integrated wireless local area network (WLAN) and wireless wide area network (WWAN), where WLAN can be static or mo-bile. In order to facilitate the analysis of WP-TCP performance in integrated WLAN and WWAN, we first construct a novel WLAN link model, which captures the impact of both uncor-related and correlated transmission errors, and derive mathematical expressions that describe packet loss probability and packet loss burst length over WWAN-WLAN link. Then, we develop analytical frameworks for studying the performance of WP-TCP short- and long-lived flows. Differently from those reported in the literature, our analytical framework for WP-TCP short-lived flows takes into account both correlated and uncorrelated packet losses. Furthermore, our analytical framework for long-lived flow can be used to study the short-term (during vertical handover) and long-term performances of WP-TCP and it captures the effects of vertical handover, such as excessive packet losses and sudden change in network characteristics, which are commonly experienced in integrated static WLAN and WWAN. By using the devel-oped analytical frameworks, we extensively analyze the performance of WP-TCP flows and in-vestigate the optimal protocol design parameters over a wide range of network conditions. Finally, based on our analytical studies, we propose a receiver-centric loosely coupled cross-layer design along with two proactive schemes, which significantly improve the vertical hand-over performance. The proposed solution is easy to implement and deploy, compatible with tra-ditional TCP, and robust in the absence of cross-layer information. Extensive simulations have been conducted to confirm the effectiveness and practicability of our schemes.

Recently, wireless access to Internet applications and services has attracted a lot of attention. However, there is no single wireless network that can meet all mobile users’ requirements. Con-sequently, integrated heterogeneous wireless networks are introduced to meet diverse wireless Internet applications and services requirements. On the other hand, integrated heterogeneous wireless networks pose new challenges to the design and development of reliable transport mechanisms. Wireless Application Protocol version 2 (WAP 2.0) is one of the promising trans-port mechanisms. It uses wireless profiled TCP (WP-TCP), which is fully compatible with TCP, as one of the reliable transport protocols to cope with the wireless link impairments. For WAP 2.0 to continue providing reliable and efficient transport services in the future, one of the key is-sues is to thoroughly study, understand, and improve its performance in integrated heterogeneous wireless networks. In this thesis, we develop analytical frameworks and propose a solution to respectively study and improve the performance of WP-TCP in integrated heterogeneous wireless networks. Spe-cifically, we consider WP-TCP short- and long-lived flows over integrated wireless local area network (WLAN) and wireless wide area network (WWAN), where WLAN can be static or mo-bile. In order to facilitate the analysis of WP-TCP performance in integrated WLAN and WWAN, we first construct a novel WLAN link model, which captures the impact of both uncor-related and correlated transmission errors, and derive mathematical expressions that describe packet loss probability and packet loss burst length over WWAN-WLAN link. Then, we develop analytical frameworks for studying the performance of WP-TCP short- and long-lived flows. Differently from those reported in the literature, our analytical framework for WP-TCP short-lived flows takes into account both correlated and uncorrelated packet losses. Furthermore, our analytical framework for long-lived flow can be used to study the short-term (during vertical handover) and long-term performances of WP-TCP and it captures the effects of vertical handover, such as excessive packet losses and sudden change in network characteristics, which are commonly experienced in integrated static WLAN and WWAN. By using the devel-oped analytical frameworks, we extensively analyze the performance of WP-TCP flows and in-vestigate the optimal protocol design parameters over a wide range of network conditions. Finally, based on our analytical studies, we propose a receiver-centric loosely coupled cross-layer design along with two proactive schemes, which significantly improve the vertical hand-over performance. The proposed solution is easy to implement and deploy, compatible with tra-ditional TCP, and robust in the absence of cross-layer information. Extensive simulations have been conducted to confirm the effectiveness and practicability of our schemes.

Uppsatsen handlar om tolv unga högstadietjejers syn på sig själva som varandes tjejer och hur detta påverkar deras skolsituation. De övergripande frågorna har rört sig kring vilka könsmarkörer tjejerna använder för att definiera sig som tjejer men också hur dessa används normativt samt vilken betydelse de får i relation till andra tjejer. Det är tjejernas röst kring frågor om att vara tjej idag som ska få träda fram i denna uppsats. Studien är en kvalitativ studie, baserad på frivilligt medverkande informanter i en förortsskola i Stockholm under läsåret 2007/2008. Intervjuerna genomförda enskilt och i par, de är inspelade på band och efter transkriberade till text för att skydda informanternas anonymitet.  Informanterna talar om en norm som är svårgripbar för dem att beskriva men den unga tjej som bryter mot denna norm ber om kommentarer och betraktas kanske inte som ens som tjej. Normen skapas genom ett samspel mellan tjejerna där alla inblandade vet vad som gäller och vilket pris man betalar om man bryter mot normen. Normen skapas i det homosociala rummet, mellan tjejerna i deras vardag och är ständigt rådande. Studien utgår från intervjuer och den genomgående tanken är att studera det socialt konstruerade könet, hur det ser ut i denna grupp av unga tjejer och hur det sedan påverkar tjejernas beteenden gentemot varandra, den sociala interaktionen. Utifrån intervjuerna har svaren sedan analyserats och jämförts mot nyare forskning och olika genus teorier. Under intervjuerna användes frågor som rörde flera olika områden och arbetet är upplagt på så sätt att varje frågeområde redovisas för sig. Efter varje frågeområde följer en analys av svaren som också anknyter till den forskning som i dagsläget finns inom området. Varje delområde redovisas med en analysdel som kopplas till den forskning jag har funnit på området samt till de aktuella genusteorier som finns. Sist i arbetet ligger en sammanfattande analys av alla de delområden som analyserats under varje avsnitt. / Slapper, geek, bimbo or just a regular girl –identity  construction for young Swedish females. The aim of this dissertation is to study twelve young schoolgirls, to see how they view themselves as girls, and how this affects their situation at school. The main aim is to let the girls themselves voice how they perceive what it is to be a young girl today; what defines a girl, what are the specific gender markers, underlying norm system, and how does this in turn affect behaviour within the group with regards to responsibility, fashion, sub groups and role models. The emphasis of the study is to observe and analyse socially constructed gender norms, do they exist among this group, and if so, how does this affect the girls’ behaviour and social interaction? The study is a qualitative study, based on a selected group of volunteers from a secondary school in a Stockholm suburb during the academic year of 2007-2008. The students were interviewed separately and in groups, and the transcribed interviews have then been compared to other resent studies of similar demographic groups, and viewed and analysed in the light of resent research theories about gender and gender construction. Every cluster of questions regarding each specific research area is dealt with separately, with an analysis of the answers and comparisons to other theories and findings finishing off each section. The main claims from analysing the material, would be that it is important to live up to the socially created and culturally perceived norm, to adhere to the gender markers and not break the invisible mould. All the interviewed girls were very aware of the norm system surrounding them, and they also know that if you break the rules of what is expected of a girl, you can expect negative consequences, and might not even be perceived as a “real” girl. The norms are created and sustained in a social interaction, between the girls but also in interaction with the opposite sex. Some of the rules can appear arbitrary to the detached observer, but for the group of girls the rules are self evident and unquestionable. The norms are mostly created in the homo-social sphere, between the girls in their everyday lives, and can be hard to describe in detail, even for the informants themselves, although they can describe it clear enough when they perceive that the rules brought on by the norm has been broken. The thesis ends with a summary and analysis of all the previous sections, and with suggestions for possible further areas of research.

Un défi mondial fondamental est de développer des technologies peu coûteuses et stables pour récolter efficacement l'énergie solaire et la transformer en formes pratiques. Ainsi pour la conversion photovoltaïque plusieurs générations de cellules solaires ont émergé. En général, on peut diviser les types existants de cellules solaires en deux classes distinctes: les photovoltaïques inorganiques conventionnels (IPV), comme les jonctions silicium p-n, et les cellules solaires excitoniques (XSCs). Selon le type de matériaux utilisés les cellules solaires excitoniques sont classées en deux catégories: les cellules solaires à colorant (DSC) et les cellules organiques (OPV) développées en couche unique, ou en bi-couche, et les hétérojonction en volume (BHJ). Les cellules solaires à base de points quantiques (QDSC) sont un autre type de cellules solaires qui ont une configuration similaire aux DSCs ou OPVs.Bien que la performance des cellules solaires excitoniques ait été un thème central de la communauté scientifique pendant de nombreuses années, des approches théoriques facilitant sa compréhension sont nécessaires. Les théories semi-classiques son inadaptées pour traiter les phénomènes quantiques dans les cellules solaires nano-structurées. De plus, en raison de l'attraction coulombienne entre les porteurs photo-générés, l'application du formalisme de la fonction de Green hors équilibre (NEGF) pose certaines difficultés. Par conséquent, dans cette thèse, nous développons un nouveau formalisme quantique, basé sur la théorie de la diffusion quantique et sur l'équation de Lippmann-Schwinger, pour fournir un cadre complet pour comprendre les processus fondamentaux intervenant dans le fonctionnement des cellules solaires excitoniques.En particulier, nous nous concentrons sur des aspects qui ont été peu pris en compte dans le passé et nous abordons, au travers d’un modèle à deux niveaux, l'interaction Coulombienne électron-trou à courte et à longue portée, la recombinaison électron-trou, l'existence de canaux d'évacuation supplémentaires, le couplage électron phonon et la formation de bandes polaroniques.Ici, les cellules solaires excitoniques à deux niveaux sont considérées dans les régimes permanents et transitoires d'injection de charge. Les photocellules moléculaires où le processus de conversion de l'énergie se déroule dans un seul complexe donneur-accepteur moléculaire attaché aux électrodes sont considérées comme étant représentatives des XSC dans le régime permanent. A titre d'exemple pour les dispositifs photovoltaïques dans le régime transitoire, nous considérons les cellules photovoltaïques organiques hétéro-jonctions massives (BHJ OPV) qui sont l'approche la plus courante des OPV et se composent d'espèces mixtes donneuses et accepteuses. Dans ces systèmes, l'exciton créé par l'absorption des photons dans le côté donneur doit atteindre d'abord l'interface donneur-accepteur. A partir de ce moment, seulement un régime transitoire commence où les charges peuvent être séparées et injectées dans leurs côtés respectifs.Nous démontrons que la séparation du porteur de charge est un processus complexe qui est affecté par différents paramètres, tels que la force de l'interaction électron-trou et le taux de recombinaison non radiative. En outre, en fonction de la structure de la cellule, l'interaction électron-trou peut normalement diminuer ou augmenter anormalement l'efficacité. Le modèle proposé aide à comprendre les mécanismes des cellules solaires excitoniques, et il peut être utilisé pour optimiser leur rendement. / A fundamental global challenge is to develop an inexpensive, stable and scalable technology for efficiently harvesting solar photon energy and converting it into convenient forms. Photovoltaic energy conversion is attracting great attention such that several generations of solar cells have emerged. The existing types of solar cells roughly fall into two distinct classes: conventional inorganic photovoltaics (IPVs), such as silicon p-n junctions, and excitonic solar cells (XSCs). The mechanistic distinction of IPVs and XSCs results in fundamental differences in their photovoltaic behavior.According to the type of materials used in their structure, excitonic solar cells are classified into two categories: dye-sensitized solar cells (DSC) and organic photovoltaics (OPV) developed in single-layer and bi-layer including planar and bulk hetero--junction configurations. Quantum dot solar cells (QDSC) are another type of solar cells that have a similar configurations to DSCs or OPVs.While understanding the performance of excitonic solar cells has been a central effort of the scientific community for many years, theoretical approaches facilitating the understanding of electron-hole interaction and recombination effects on the cell performance are needed. Semiclassical theories are inefficient tools to treat quantum phenomena in nano-structured solar cells, and on the other hand, due to the Coulomb attraction between the photo generated carriers, the application of standard Non-Equilibrium Green Function (NEGF) formalism presents some difficulties although some specific methods allow to circumvent this problem.In this thesis we develop a new quantum formalism, which is based on quantum scattering theory and on the Lippmann-Schwinger equation, to provide a comprehensive framework for understanding the fundamental processes taking place in the operation of excitonic solar cells. Considering simple two-level models we address important effects such as the short--range and long--range electron--hole Coulomb interaction, the electron--hole recombination, the existence of extra evacuation channels, and the electron--phonon coupling and polaronic bands formation.Here, the two-level excitonic solar cells are considered in the permanent and transitory regimes of charge injection. The molecular photocells where the energy conversion process takes place in a single molecular donor-acceptor complex attached to electrodes are considered as a representative of XSCs in the permanent regime. As an example for the photovoltaic devices in the transitory regime, we consider the bulk hetero--junction organic photovoltaic cells (BHJ OPVs) which are the most common approach to OPVs and consists of mixed donor and acceptor species that form interpenetrating connective networks. In these systems the exciton created by the photon absorption in the donor side must reach first the donor--acceptor interface. From this moment only a transitory regime begins where the charges can be separated and injected in their respective sides.We demonstrate that the charge carrier separation is a complex process that is affected by different parameters, such as the strength of the electron--hole interaction and the non--radiative recombination rate. Furthermore, depending on the cell structure, the electron-hole interaction can normally decrease or abnormally increase the cell efficiency. The proposed model helps to understand the mechanisms of excitonic solar cells, and it can be used to optimize their yield.

The aim of the study has been to examine women's participation in the reproduction of the system of heteronormative expectations of femininity and masculinity through narrative method, analyze books within the erotic women’s literature, as well as interviews with females. The study's focus was on the presentation and representations of the femininity(s) and masculinity(s). Through the questions , How represents heteronormative femininity(s) and masculinity(s) in the erotic women´s litterateur? How portray women, their views on femininity(s) and masculinity(s)? The study were linked with the radical feminist theory regarding the male patriarchate's role as a hegemony in society, as well as one of queer theory's definitions of the gender and heteronormativity. The study also included elements of feminist research on popular culture. On the basis of my purpose and the research question I have come to the conclusion that women are involved in the reproduction of the system of heteronormative expectations of femininity and masculinity. But the study also shows a shifting duality of " ideal woman" and "ideal man". / Studiens syfte har varit att undersöka kvinnors delaktighet i reproduktion av heteronormativa förväntningar på kvinnlighet och manlighet genom narrativmetod, analysera böcker inom erotisk kvinnolitteratur samt intervjuer med kvinnor. Studiens fokus handlade om samtidens framställande samt representationer av femininitet(er) och maskulinitet(er). Genom frågeställningarna, Hur framställs heteronormativ femininitet(er) och maskulinitet(er) i erotisk kvinnolitteratur? Hur framställer kvinnor sin syn på femininitet(er) och maskulinitet(er)? Studien kopplades samman med radikal feministisk teori rörande det manliga patriarkatets roll som hegemoni i samhället samt queerteorins definitioner av genus och heteronormativitet. Studien innefattade också inslag av feministisk forskning rörande populärkulturen. Utifrån mitt syfte samt frågeställning har jag kommit fram till att kvinnor är delaktiga i reproduktionen av heteronormativa förväntningar på kvinnlighet och manlighet. Men studien visar också på en skiftande dubbelhet rörande "idealkvinnan" och "idealmannen".

In recent years, the use of first-principles based atomistic modeling technique has become extremely popular to gain better insights on the various locally modulated electronic properties of nano materials and structures. Atomistic modeling offers the benefit of predicting crystal structures, visualizing orbital distribution and electron density, as well as understanding material properties which are hard to access experimentally. The single layer MoS2 has emerged as a suitable choice for the next generation nano devices, owing to its distinctive electrical, optical and mechanical properties like, better electrostatics, increased photo luminescence, higher mechanical flexibility, etc. The realization of decananometer scale digital switches with the single layer MoS2 as the channel may provide many significant advantages such as, high On/Off current ratio, excellent electrostatic control of the gate, low leakage, etc. However, there are quite a few critical issues such as, forming low resistance source/drain contacts, achieving higher effective mobility, ensuring large scale controlled growth, etc. which need to be addressed for successful implementation of the atomically thin transistors in integrated circuits. Recent experimental demonstration showing the coexistence of metallic and semiconducting phases in the same monolayer MoS2, has attracted much attention for its use in ultra-low contact resistance-MoS2 transistors. Howbeit, the electronic structures of the metallic-to-semiconducting phase boundaries, which appear to dictate the carrier injection in such transistors, are not yet well understood. In this work, we first develop the geometrically optimized atomistic models of the 2H-1T′ hetero-phase structures with two distinct phase boundaries, β and γ. We then apply density functional theory to calculate the electronic structures for those optimized geometries. Furthermore, we employ non equilibrium Green’s function formalism to evaluate the transmission spectra and the local density of states in order to assess the Schottky barrier nature of the phase boundaries. Nonetheless, the symmetry of the source-channel and drain-channel junction, is a unique property of a metal-oxide semiconductor field effect transistor (MOSFET), which needs to be preserved while realizing sub-10 nm channel length devices using advanced technology. Employing experimental-findings-driven atomistic modeling technique, we demonstrate that such symmetry might not be preserved in an atomically thin phase-engineered MoS2- based MOSFET. It originates from the two distinct atomic patterns at phase boundaries (β and β*) when the semiconducting phase (channel) is sandwiched between the two metallic phases (source and drain). Next, using first principles based quantum transport calculations we demonstrate that due to the clusterization of “Mo” atoms in 1T′ MoS2, the transmission along the zigzag direction is significantly higher than that in the armchair direction. Moreover, to achieve excellent impedance matching with various metal contacts (such as, “Au”, “Pd”, etc.), we further develop the atomistic models of metal-1T′ MoS2 edge contact geometries and compute their resistance values. Other than the charge carrier transport, analysing the heat transport across the channel is also crucial in designing the ultra-thin next generation transistors. Hence, in this thesis work, we have investigated the electro-thermal transport properties of single layer MoS2, in quasi ballistic regime. Besides the perfect monolayer in its pristine form, we have also considered various line defects which have been experimentally observed in mechanically exfoliated MoS2 samples. Furthermore, a comprehensive study on the phonon thermal conductivity of a suspended monolayer MoS2, has been incorporated in this thesis. The studies presented in this thesis could be useful for understanding the carrier transport in atomically thin devices and designing the ultra-thin next generation transistors.

Syftet med denna studie är att bidra med kunskap om svenskundervisning ur ett hbtq-perspektiv i fyra olika grundskolor, varav två är hbtq-certifierade, samt att dessutom närmare undersöka lärares kunskaper om och upplevelse av att arbeta med hbtq-perspektiv i svenskundervisning. Jag har studerat hur lärarna arbetade, vilka som representerades i läromedlen och i klassrumsmiljöerna, hur klassrummen var möblerade samt hur eleverna var grupperade. Fyra skolor, varav två genomgått RFSL:s hbtq-certifiering, ingår i undersökningen. Studien visar att det råder brist på kunskap om hbtq hos två av de fyra lärarna. Normkritiskt arbetssätt är inte något som praktiseras kontinuerligt förutom av en lärare som ibland hade inslag av normkritiskt arbete. Ingen av de fyra lärarna hade berört hbtq under sina lärarutbildningar. Slutsatsen av mitt arbete är att det krävs en kompetenshöjning när det gäller hbtq-relaterade frågor hos personal som jobbar i skolan. Att hbtq-certifiera verksamheten verkar inte ge bestående effekt; det krävs uppföljning och kontinuerligt arbete för att ändra på de starka normer som råder i samhället. Då styrdokumenten tydligt förespråkar en inkludering av alla elever oavsett kön, könsöverskridande identitet eller uttryck, etnisk tillhörighet, religion eller annan trosuppfattning, funktionsnedsättning, sexuell läggning eller ålder borde kraven på kompetenshöjning i ett normkritiskt arbetssätt komma via styrning uppifrån för att nå ett snabbare resultat. / The purpose of this article is to study four primary school teachers and how they work with LGBTQ-perspectives. Four schools, of which two are LGBTQ-certified by RFSL, are included in this study. The study reveals a lack of knowledge regarding LBTQ issues in three out of the four teachers. Norm critical practices are not continuously applied by the teachers in their work, with the exception of one who does apply them. None of the teachers had come in to contact with LGBTQ through their own education. The conclusion I have drawn from my work on this article is that an improvement of competence concerning LGBTQ related issues among personnel working in school environments is required. LGBTQ-certification does not seem to come with a lasting effect; follow-ups and continuous work are required to change the strong norms that are currently present in society. As Skolverket clearly advocates for the inclusion of all students, regardless of gender, cross-gender identity or expression, ethnic background, religion or other faith, disability, sexual orientation, or age, the requirements of competency improvement regarding the application of norm critical practices should come through guidance from above to assure quick results.

Septinas constituem uma família de proteínas de ligação a GTP que foram inicialmente identificadas em levedura Saccharomyces cerevisiae, mas também estão presentes em outros eucariotos com exceção de plantas. Septinas são purificadas de leveduras, Drosophila e cérebros de mamíferos na forma de filamentos, porém o mecanismo através do qual acorre a formação destes filamentos ainda não é muito bem compreendido. Septinas são constituídas de três regiões principais: um N-terminal variável, um domínio central GTPase altamente conservado e um domínio coiled-coil C-terminal. O gene SEPT4 foi identificado por M. Tanaka e colaboradores a partir do cDNA de cérebro humano e apresentou duas distintas transcrições: Bradeiona ? e ?. Interessantemente, além de cérebro e coração, as proteínas Bradeiona Α e Β. são detectadas somente em câncer do cólon, reto, próstata e melanoma maligno. Neste trabalho, o gene da proteína Bradeiona Β foi subclonado em um vetor de expressão bacteriano, produzido em E. coli e purificado com sucesso. O espectro de dicroísmo circular (CD) mostrou o perfil característico de proteínas com hélices a na estrutura secundária. Resultados de cromatografia de exclusão molecular (SEC) e espalhamento dinâmico de luz (DLS) indicam que a septina Bradeina foi produzida na forma de um estável oligômero com características monodispersivas, que foi subseqüentemente cristalizado em PEG6000. A atividade GTPase da Bradeiona Β foi comprovada através da técnica de eletroforese capilar (CE), mostrando-se absolutamente dependente de íons Mg2+. Inibição da atividade GTPase foi verificada em altas concentrações de Mg2+ (maiores que 5 mM). Com a finalidade de caracterizar os domínios preditos da Bradeiona Β (Fragmento Conservado e domínio GTPase), essas regiões foram previamente definidas, expressas em E. cozi e purificadas com sucesso. Resultados de CD, SEC, espectroscopia de fluorescência e NMR-600MHz indicam que o FC foi produzido na forma de um estável monômero com pouca estrutura secundária regular. Resultados de DLS e CD indicam que a fusão 6xHis-DGTPase foi produzida na forma de um oligômero com a presença de hélices a na estrutura secundária. A fusão 6xHis-DGTPase mostrou-se instável a altas concentrações na ausência de imidazol. A atividade GTPase da fusão GST+DGTPase foi comprovada, similarmente a Bradeiona , através da técnica de CE. Novamente, verificou-se dependência de íons Mg2+ (para a atividade catalítica) e inibição em altas concentrações de Mg2+. A fusão GST+DGTPase também foi capaz de hidrolisar ATP. Espera-se que as informações relatadas neste estudo proporcionem um alicerce para estudos estruturais/funcionais futuros das proteínas Bradeiona Α e Βoutras septinas. / Septins form a class of eukaryoyic guanine nucleotide-binding proteins that were first identified in budding yeast. Septins purified from yeast, Drosophila and mammalian brain form filaments, however the mechanism by which the filaments assemble is unclear. Septins have a highly conserved structure, which includes a central GTP-binding domain, a variable N-terminal region, and most septins also contain a coiled coil domain at the Cterminus. Bradeion p is one of the splice variants of the human septin gene, SEPT4, recently isolated by expression screening of an adult human brain cDNA library. The Bradeion gene resides at 17q23, and has been shown to present specific expression in both human colorectal cancer, urologic cancers and malignant melanoma. In order to characterize the GTPase activity of Bradeion Β , the protein was successfully expressed in E. coli and purified. The recombinant protein was characterized by circular dichroism (CD), dynamic light scattering (DLS) and a novel non-radioactive enzyme assay, which utilizes capillary electrophoresis (EC) to monitor GTP hydrolysis. The CD spectrum exhibited the typical shape characteristic of the presence of helical elements of secondary structure and the DLS pattern was indicative of a monodisperse solution, which was readily crystallized in the presence of PEG6000. GTP hydrolysis was shown to be Mg2+ dependent within the low millimolar range but at 5 mM was inhibitory. In order to characterize the predicted domains of Bradeion Β, these defined regions were successfully expressed in E. cozi and purified. The CD spectrum of CF exhibited the shape typically found for non-regular structure. The results of fluorescence spectroscopy, gel filtration (SEC) and NMR-600MHz also corroborate with the CD results indicating an irregular structure. The fusion protein 6xHis-DGTPase exhibited a CD spectrum with the typical shape characteristic of the presence of helical elements but was show to be instable at high concentrations in the absence of imidazole. To characterize the GTPase activity of the fusion protein GST+DGTPase, the CE technique was used to monitor GTP hydrolysis. Analysis by CE showed that GST+DGTPase was functional, since both GTP and ATP hydrolysis was observed in a Mg2+ dependent manner. This work provides novel approaches, which should aid in the fbture study of the structure and fùnction of Bradeion Α e Β, others septins and related GTPases.