Diatoms are single-celled microalgae with silica-based cell walls (frustules) that are abundantly present in aquatic habitats, and form the basis of the food chain in many ecosystems. Many benthic diatoms have the remarkable ability to glide on all natural or man-made underwater surfaces using a carbohydrate- and protein-based adhesive to generate traction. Previously, three glycoproteins, termed FACs (Frustule Associated Components), have been identified from the common fouling diatom Craspedostauros australis and were implicated in surface adhesion through inhibition studies with a glycan-specific antibody. The polypeptide sequences of FACs remained unknown, and it was unresolved whether the FAC glycoproteins are indeed involved in adhesion, or whether this is achieved by different components sharing the same glycan epitope with FACs. Here we have determined the polypeptide sequences of FACs using peptide mapping by LC–MS/MS. Unexpectedly, FACs share the same polypeptide backbone (termed CaFAP1), which has a domain structure of alternating Cys-rich and Pro-Thr/Ser-rich regions reminiscent of the gel-forming mucins. By developing a genetic transformation system for C. australis, we were able to directly investigate the function of CaFAP1-based glycoproteins in vivo. GFP-tagging of CaFAP1 revealed that it constitutes a coat around all parts of the frustule and is not an integral component of the adhesive. CaFAP1-GFP producing transformants exhibited the same properties as wild type cells regarding surface adhesion and motility speed. Our results demonstrate that FAC glycoproteins are not involved in adhesion and motility, but might rather act as a lubricant to prevent fouling of the diatom surface.

No description available.

Cette thèse est scindée en deux parties. Dans un premier temps, nous présentons deux schémas volumes finis pour la discrétisation des problèmes de diffusion-convection-réaction sur les surfaces mouvantes. Le premier schéma présente une extension du schéma volumes finis avec flux à deux points sur les surfaces mouvantes. Le deuxième développe une méthode de type O-méthode. Cette dernière consiste à construire à partir des inconnus au centre des mailles, des solutions linéaires autour des nœuds de maillage qui intègrent la continuité des flux aux interfaces de mailles. La méthode permet aussi la construction des décentrages amont d'ordre 2 et ainsi, offre au procédé l'ordre 2 de convergence sur tout maillage non dégénéré. Ensuite, nous modélisons l'écoulement du couplage filme mince-surfactant (surface active agent) sur les surfaces mouvantes et simulons à l'aide des schémas volumes finis précédemment définis. Ici, l'utilisation du calcul tensoriel et de la méthode d'approximation par la lubrification permettent de réduire les équations de Navier-Stokes caractérisant le mouvement du filme mince en dimension 3 en un système d'équations définies sur la surface courbe mouvante dont l'inconnu est la hauteur du fluide. Le surfactant supposé insoluble est modélisé par une équation de diffusion convection à la l'interface fluid-air. Nous simulons l'ensemble en utilisant une méthode dite de capture d'interface (Interface tracking method) dérivée des volumes finis définis plus haut. Plusieurs exemples illustrent à suffisance l'efficacité et la précision des différentes méthodes.

The main objective of this thesis work was to gain understanding of the principles of biolubrication, focusing on synergistic effects between biolubricants. To this end surface force and friction measurements were carried out by means of Atomic Force Microscopy, using hydrophilic and hydrophobic model surfaces in salt solutions of high ionic strength (≈ 150 mM) in presence of different biolubricants. There was also a need to gain information on the adsorbed layers formed by the biolubricants. This was achieved by using a range of methods such as Atomic Force Microscopy PeakForce imaging, Quartz Crystal Microbalance with Dissipation, Dynamic Light Scattering and X-Ray Reflectometry. By combining data from these techniques, detailed information about the adsorbed layers could be obtained.The biolubricants that were chosen for investigation were a phospholipid, hyaluronan, lubricin, and cartilage oligomeric matrix protein (COMP) that all exist in the synovial joint area. First the lubrication ability of these components alone was investigated, and then focus was turned to two pairs that are known or assumed to associate in the synovial area. Of the biolubricants that were investigated, it was only the phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) that was found to be an efficient lubricant on its own. Deposited DPPC bilayers on silica surfaces were found to be able to provide very low friction coefficients (≈ 0.01) up to high pressures, ≈ 50 MPa. A higher load bearing capacity was found for DPPC in the liquid crystalline state compared to in the gel state.The first synergy pair that was explored was DPPC and hyaluronan, that is known to associate on the cartilage surface, and we also noticed association between hyaluronan and DPPC vesicles as well as with adsorbed DPPC bilayers. By combining these two components a lubrication performance similar to that of DPPC alone could be achieved, even though the friction coefficient in presence of hyaluronan was found to be slightly higher. The synergy here is thus not in form of an increased performance, but rather that the presence of hyaluronan allows a large amount of the phospholipid lubricant to accumulate where it is needed, i.e. on the sliding surfaces.The other synergy pair was lubricin and COMP that recently has been shown to be co-localized on the cartilage surface, and thus suggested to associate with each other. Lubricin, as a single component, provided poor lubrication of PMMA surfaces, which we utilized as model hydrophobic surfaces. However, if COMP first was allowed to coat the surface, and then lubricin was added a low friction coefficient (≈ 0.03) was found. In this case the synergy arises from COMP facilitating strong anchoring of lubricin to the surface in conformations that provide good lubrication performance. / Huvudsyftet med det här avhandlingsarbetet var att öka förståelsen för den låga friktion som finns i vissa biologiska system, med fokus på synergistiska effekter mellan de smörjande molekylerna. För detta ändamål studerades ytkrafter och friktion med hjälp av atomkraftsmikroskopi. Mätningarna utfördes med hydrofila och hydrofoba modellytor i lösningar med hög salthalt (≈ 150 mM) i närvaro av smörjande biomolekyler. Det var också nödvändigt att få information om de adsorberade skikten av biomolekyler. Det åstadkoms med hjälp av en rad tekniker så som AFM PeakForce avbildning, kvartskristallmikrovåg, dynamisk ljusspridning och röntgen reflektometri. Genom att kombinera data från dessa tekniker erhölls detaljerad information om de smörjande skikten.De smörjande biomolekyler som valdes ut för studierna var en fosfolipid, hyaluronan, lubricin, and cartilage oligomeric matrix protein (COMP) vilka alla finns i synovialledsområdet. Först undersöktes den smörjande förmågan hos dessa komponenter var för sig, och sedan fokuserade vi på två par av biomolekyler som man vet eller antar bildar associationsstrukturer i synovialleder. Av de enskilda biomolekyler som undersöktes var det endast fosfolipiden 1,2-dipalmitoyl-sn-glycero-3-fosfokoline (DPPC) som visade sig vara en effektivt smörjande molekyl. Deponerade biskikt av DPPC på silikaytor gav upphov till mycket låga friktionskoefficienter (≈ 0.01) upp till höga pålagda tryck, ≈ 50 MPa. DPPC bilager i flytande kristallin fas visade sig ha högre lastbärande förmåga än DPPC bilager i geltillstånd.Det första synergistiska par som undersöktes var DPPC och hyaluronan vilka man vet associerar på broskytan, och vi visade att hyaluronan associerar med såväl DPPC vesiklar som med DPPC bilager. Genom att kombinera dessa två komponenter uppmättes en smörjande förmåga som var jämförbar med den som DPPC ensam uppvisar. Även om friktionskoefficienten var något högre i närvaro av hyaluronan. Synergieffekten här består inte av en bättre smörjande förmåga, utan istället gör närvaron av hyaluronan att de smörjande fosfolipiderna kan ansamlas i stora mängder där de behövs, dvs. på de glidande ytorna.Det andra synergiparet var lubricin och COMP vilka nyligen har visats vara lokaliserade på samma platser på broskytan, vilket tyder på att de associerar med varandra. På egen hand var lubricins smörjande förmåga av PMMA, våra hydrofoba modellytor, dålig. Emellertid, om COMP först adsorberades på PMMA och sedan lubricin tillsattes uppmättes en låg friktionskoefficient (≈ 0.03). I det här fallet består synergin av att COMP möjliggör en stark inbindning till ytan av lubricin i konformationer som ger god smörjande förmåga. / <p>QC 20141202</p> / Stiftelsen för strategisk forskning - SSF

Dans l’industrie, les fabricants tendent à limiter la lubrification conventionnelle lors de l’usinage et s’orientent de plus en plus vers la microlubrification (MQL : Minimum Quantity Lubrication) pour des raisons économiques, écologiques et pour respecter la santé des opérateurs. Une très faible quantité de lubrifiant de faible pression nécessite une conception optimale des canaux internes de l’outil afin de limiter les pertes de charges et avoir les avantages de la microlubrification. Premièrement, une revue bibliographique introduit l’étude en présentant les avantages qu’induit le passage à la microlubrification. Par la suite, quelques conceptions d’outils avec une lubrification interne qui vise la face de coupe ou la face de dépouille de la plaquette sont passées en revue. / In machining industry, manufacturers tend to limit conventional lubrication and are moving increasingly towards Minimum Quantity Lubrication (MQL) for economic and ecological reasons, and with a view to safeguarding operator health and safety. A very small amount of lubricant at low pressure requires optimized design of the tools internal channels in order to minimize pressure drops and gain benefit from MQL. First, a literature review introduces the study by presenting the benefits induced by switching to MQL. Thereafter, some tool designs with internal lubrication for rake face or flank face lubrication are presented.

Abstract In the world where every sector of industrial manufacturing is being converted toautomated systems, surface finishing processes like sanding and polishing seem to lag.This phenomenon is not surprising as these processes are complex to optimize. Therehave been projects going on with the support of European Commission to findsolutions under SYMPLEXITY (Symbiotic Human-Robot Solutions for ComplexSurface Finishing Operations). One of the projects in under this include poliMATIC(Automated Polishing for the European Tooling Industry). Halmstad University isinvolved in doing projects. This project took a portion of this study in aim to understand a foam material’s behavior used for sanding tool at the tip of a robotic arm. This is studied using a forcemeasurement system developed at Halmstad University. The project has two sectionsand starts with one; Understanding the force measurement system and upgrading innecessary ways. Two; studying how the foam material compressive hardness propertyis affected when the material is fit with sandpaper for sanding operation using theforce measurement system. The study finally revealed how the combination of thefoam with sandpaper affects the robustness of the material, and significantlyimproved the output of the system with by reducing the noise level with 40%.

O presente trabalho discute a influência da microestrutura dos ferros fundidos, cinzento (FFC) e vermicular (FFV), na ocorrência de metal dobrado (FM) em superfícies brunidas e no comportamento tribológico em ensaio lubrificado do tipo anel sobre cilindro. As amostras de ferro fundido foram extraídas diretamente de um bloco de motor de combustão interna, em regiões de diferentes espessuras. O anel de pistão utilizado foi de aço inoxidável martensítico nitretado com perfil assimétrico e o óleo lubrificante foi o SAE 30 CF monoviscoso. A quantificação de sulcos e de metal dobrado (FM) foi realizada empregando-se uma metodologia baseada na literatura. Para a execução dos ensaios tribológicos foi projetada e construída uma adaptação para a montagem das amostras e controle de temperatura do óleo lubrificante. Um parâmetro para avaliação do desempenho tribológico foi proposto – índice de mérito tribológico (IMT) – que leva em conta coeficiente de atrito (COF) e alterações de topografia. Os ferros fundidos apresentaram microestruturas típicas. Contudo, observaram-se diferenças de morfologia e distribuição da grafita; com maior número de grafitas e menor fração de grafita para os materiais de parede fina. Observou-se FM nos platôs e nos sulcos das superfícies brunidas em todas as amostras. Quantificações mostraram que as larguras dos sulcos foram estatisticamente iguais e com alto percentual de obstrução. A quantificação de FM dos materiais mostrou maiores valores presentes na região do Spk (FMspk) para os FFC's. Os materiais de parede grossa apresentaram maiores valores de % FM dentro do sulco (%FM/Sulco) e este parâmetro teve forte e positiva correlação estatística com a fração de grafita. Evidências, obtidas em avaliações de seções transversais, corroboraram a relação entre FM e grafita. Constatou-se que o FM pode ser formado diretamente sobre a grafita ou, ainda, de forma indireta devido à extrusão desta fase. Ademais, verificou-se que a morfologia e orientação da grafita têm influência na formação do FM. Realizaram-se ensaios preliminares de curta duração de anel sobre cilindro para a avaliação da repetibilidade dos resultados de COF, os quais indicaram menor dispersão para condições com menores frequências e maiores forças normais. Avaliações da dinâmica do ensaio tribológico permitiram apontar relações de redução de COF durante os semi-ciclos de movimento e associá-las à teoria de lubrificação hidrodinâmica. O COF para ensaios de longa duração apresentou comportamento cíclico com transições. Estas transições foram associadas a mecanismos de formação-remoção-formação de tribofilmes de ZDDP. Os ensaios impuseram alterações nos parâmetros de rugosidade (principalmente com alisamento das superfícies) e redução do parâmetro de filme. O IMT evidenciou pouca influência da espessura do material; e melhor desempenho tribológico para os FFV's. A rotina de quantificação de FM mostrou limitação técnica devido ao alisamento da superfície, o que causa mudança na referência de alturas das superfícies brunidas. Ainda assim, verificou-se redução do %FM/Sulco e tendência de desobstrução do sulco. Contatou-se pouca influência do FM no comportamento do COF devido à similaridade dos parâmetros de FM entre os materiais. Contudo, o FM foi associado a um agente de abrasão à três corpos e apresentou forte correlação positiva do parâmetro de FMspk (no início do ensaio) com o parâmetro do IMT relacionado com a alteração de topografia. / The present work discusses the influence of the microstructure of gray cast iron (GCI) and compacted graphite iron (CGI) on the occurrence of folded metal (FM) on honed surfaces and its tribological behavior in ring-on-cylinder test under lubrication. The cast iron samples were taken directly from an internal combustion engine block in regions of different thicknesses. The piston ring used was a nitrided martensitic stainless steel with asymmetrical profile and the lubricant oil was the SAE 30 CF. The quantification of grooves and folded metal (FM) was carried out using a methodology based on the literature. For the execution of the tribological tests an adaptation was designed and built for assembling the samples and for controlling the oil temperature. One parameter to evaluate the tribological performance was proposed - tribological merit index (TMI) - which considers the coefficient of friction (COF) and changes in topography. Each cast iron presented typical microstructures. However, differences in morphology and graphite distribution were observed; the samples from thinner wall presented higher number of graphite and less fraction of graphite. FM was observed on the plateaus and grooves of the honed surfaces in all samples. Quantification showed that the widths of the grooves were statistically similar and with a high percentage of obstruction. GCIs present a higher amount of FM in the Spk region (FMspk).The thicker wall samples presented a higher amount of FM in the grooves (% FM / Groove) and this parameter had a strong and positive statistical correlation with the graphite fraction. Evidences, obtained in cross section evaluations, corroborated the relationship between FM and graphite. It was verified that FM can be formed directly on graphite or indirectly due to the extrusion of this phase. In addition, its morphology and orientation influenced the occurrence of FM. Preliminary short-term tests were performed to evaluate the repeatability of COF results. These tests indicated less dispersion for conditions with lower frequencies and higher normal forces. Evaluations of the dynamics of the tribological test allowed to point out conditions to reduce the COF during the half-cycles of movement and to associate them to the theory of hydrodynamic lubrication. The COF for long-term tests presented cyclical behavior with transitions. These transitions were associated with formation-removal-formation mechanisms of ZDDP tribofilms. The tribological tests imposed changes on the roughness parameters (mainly with smoothness of the surfaces) and reductions on the film parameter. The thickness of samples affected little the TMI; and a better tribological performance of CGIs was detected. The FM quantification routine showed a technical limitation due to surface smoothing, which causes a change in the reference of heights of honed surfaces. Nevertheless, there was a reduction in %FM/Groove and tendency to clear the grooves. There was little influence of FM in COF behavior due to the similarity of FM parameters among the materials. However, FM was associated as a three-body abrasive agent and a strong positive correlation between the FMspk parameter (at the beginning of the test) and the TMI parameter was described.

O presente trabalho discute a influência da microestrutura dos ferros fundidos, cinzento (FFC) e vermicular (FFV), na ocorrência de metal dobrado (FM) em superfícies brunidas e no comportamento tribológico em ensaio lubrificado do tipo anel sobre cilindro. As amostras de ferro fundido foram extraídas diretamente de um bloco de motor de combustão interna, em regiões de diferentes espessuras. O anel de pistão utilizado foi de aço inoxidável martensítico nitretado com perfil assimétrico e o óleo lubrificante foi o SAE 30 CF monoviscoso. A quantificação de sulcos e de metal dobrado (FM) foi realizada empregando-se uma metodologia baseada na literatura. Para a execução dos ensaios tribológicos foi projetada e construída uma adaptação para a montagem das amostras e controle de temperatura do óleo lubrificante. Um parâmetro para avaliação do desempenho tribológico foi proposto – índice de mérito tribológico (IMT) – que leva em conta coeficiente de atrito (COF) e alterações de topografia. Os ferros fundidos apresentaram microestruturas típicas. Contudo, observaram-se diferenças de morfologia e distribuição da grafita; com maior número de grafitas e menor fração de grafita para os materiais de parede fina. Observou-se FM nos platôs e nos sulcos das superfícies brunidas em todas as amostras. Quantificações mostraram que as larguras dos sulcos foram estatisticamente iguais e com alto percentual de obstrução. A quantificação de FM dos materiais mostrou maiores valores presentes na região do Spk (FMspk) para os FFC's. Os materiais de parede grossa apresentaram maiores valores de % FM dentro do sulco (%FM/Sulco) e este parâmetro teve forte e positiva correlação estatística com a fração de grafita. Evidências, obtidas em avaliações de seções transversais, corroboraram a relação entre FM e grafita. Constatou-se que o FM pode ser formado diretamente sobre a grafita ou, ainda, de forma indireta devido à extrusão desta fase. Ademais, verificou-se que a morfologia e orientação da grafita têm influência na formação do FM. Realizaram-se ensaios preliminares de curta duração de anel sobre cilindro para a avaliação da repetibilidade dos resultados de COF, os quais indicaram menor dispersão para condições com menores frequências e maiores forças normais. Avaliações da dinâmica do ensaio tribológico permitiram apontar relações de redução de COF durante os semi-ciclos de movimento e associá-las à teoria de lubrificação hidrodinâmica. O COF para ensaios de longa duração apresentou comportamento cíclico com transições. Estas transições foram associadas a mecanismos de formação-remoção-formação de tribofilmes de ZDDP. Os ensaios impuseram alterações nos parâmetros de rugosidade (principalmente com alisamento das superfícies) e redução do parâmetro de filme. O IMT evidenciou pouca influência da espessura do material; e melhor desempenho tribológico para os FFV's. A rotina de quantificação de FM mostrou limitação técnica devido ao alisamento da superfície, o que causa mudança na referência de alturas das superfícies brunidas. Ainda assim, verificou-se redução do %FM/Sulco e tendência de desobstrução do sulco. Contatou-se pouca influência do FM no comportamento do COF devido à similaridade dos parâmetros de FM entre os materiais. Contudo, o FM foi associado a um agente de abrasão à três corpos e apresentou forte correlação positiva do parâmetro de FMspk (no início do ensaio) com o parâmetro do IMT relacionado com a alteração de topografia. / The present work discusses the influence of the microstructure of gray cast iron (GCI) and compacted graphite iron (CGI) on the occurrence of folded metal (FM) on honed surfaces and its tribological behavior in ring-on-cylinder test under lubrication. The cast iron samples were taken directly from an internal combustion engine block in regions of different thicknesses. The piston ring used was a nitrided martensitic stainless steel with asymmetrical profile and the lubricant oil was the SAE 30 CF. The quantification of grooves and folded metal (FM) was carried out using a methodology based on the literature. For the execution of the tribological tests an adaptation was designed and built for assembling the samples and for controlling the oil temperature. One parameter to evaluate the tribological performance was proposed - tribological merit index (TMI) - which considers the coefficient of friction (COF) and changes in topography. Each cast iron presented typical microstructures. However, differences in morphology and graphite distribution were observed; the samples from thinner wall presented higher number of graphite and less fraction of graphite. FM was observed on the plateaus and grooves of the honed surfaces in all samples. Quantification showed that the widths of the grooves were statistically similar and with a high percentage of obstruction. GCIs present a higher amount of FM in the Spk region (FMspk).The thicker wall samples presented a higher amount of FM in the grooves (% FM / Groove) and this parameter had a strong and positive statistical correlation with the graphite fraction. Evidences, obtained in cross section evaluations, corroborated the relationship between FM and graphite. It was verified that FM can be formed directly on graphite or indirectly due to the extrusion of this phase. In addition, its morphology and orientation influenced the occurrence of FM. Preliminary short-term tests were performed to evaluate the repeatability of COF results. These tests indicated less dispersion for conditions with lower frequencies and higher normal forces. Evaluations of the dynamics of the tribological test allowed to point out conditions to reduce the COF during the half-cycles of movement and to associate them to the theory of hydrodynamic lubrication. The COF for long-term tests presented cyclical behavior with transitions. These transitions were associated with formation-removal-formation mechanisms of ZDDP tribofilms. The tribological tests imposed changes on the roughness parameters (mainly with smoothness of the surfaces) and reductions on the film parameter. The thickness of samples affected little the TMI; and a better tribological performance of CGIs was detected. The FM quantification routine showed a technical limitation due to surface smoothing, which causes a change in the reference of heights of honed surfaces. Nevertheless, there was a reduction in %FM/Groove and tendency to clear the grooves. There was little influence of FM in COF behavior due to the similarity of FM parameters among the materials. However, FM was associated as a three-body abrasive agent and a strong positive correlation between the FMspk parameter (at the beginning of the test) and the TMI parameter was described.

I deatta arbete har olika nötningstyper identifierats vilka entreprenadmaskiners skopor utsätts för. Utifrån de dominerande nötningstyperna ska en nötningsprovrigg konstrueras som behandlar dessa. Detta då företaget Borox som förser entreprenad och vägindustrin med slitstarka stålmaterial ska kunna testa sina material och få en relativ uppskattning om nötningsbeständighet och lämplighet på sina produkter.  En produktutvecklingsprocess följdes under arbetet och en lämplig provrigg valdes för att sedan konstrueras och ritas..

Une des voies d’amélioration des véhicules hybrides et électriques est l’utilisation de moteurs tournant plus vite, jusqu’à plus de 42.000tr/min. Le but est d’augmenter la densité de puissance et le rendement des groupes motopropulseurs. Pour utiliser ces moteurs de nouveaux réducteurs mécaniques doivent être développés. Cela doit se faire sans générer de surcoût important face aux solutions utilisées à basse vitesse et en assurant un niveau de performance énergétique élevé. Cette thèse se situe en amont de la phase de conception d’un réducteur haute vitesse lubrifié par barbotage. Elle a pour but d’identifier les problèmes d’échauffement et de pertes de puissance ainsi que de proposer des pistes d’amélioration énergétique. Ce travaille propose la modélisation thermomécanique de l’étage grande vitesse (GV) du réducteur, réalisée à l’aide de la méthode des réseaux thermiques. Ce modèle couple les pertes de puissance avec les températures. Une attention particulière est portée sur la modélisation des roulements de l’arbre GV. Un nouveau modèle thermomécanique de roulement est développé. Les pertes par barbotage deviennent importantes à grande vitesse. Une méthode permettant de fortement les réduire est caractérisée. / A way to improve both electric and hybrid vehicles is to use high speed motors, operating over than 42.000rpm. The goal is to increase the power density and the efficiency of powertrains. Using these new motors, new gearboxes should be developed. This must be done without generating significant additional cost regarding already mastered low speed solutions. High energy performance level also has to be maintained. This PhD comes before the design phase of a high-speed oil bath lubricated gearbox. It aims to identify the warm-up and power loss problems, and propose ways to improve efficiency. This work proposes a thermomechanical modelling of the gearbox’s first stage, using the thermal network method. This model links power losses with temperatures. Particular attention is paid to high speed bearing modelling. A new thermomechanical model of rolling element bearing is developed. As churning losses being significant at high speeds, a method to greatly reduce this power loss is characterized.