Approved for public release, distribution is unlimited / Test and evaluation of system performance has been a critical part of the acceptance of combat weapon systems for the Department of Defense. As combat weapon systems have become more complex, evaluation of system performance has relied more heavily on recorded test data. As part of the on-going transformation of the Defense department, Commercial-Off- The-Shelf (COTS) technology is being integrated into the acquisition of combat weapon systems. An Analysis Control Board (ACB) was created in response to these factors to support the AEGIS Weapon System Program Office. The focus of this ACB was to investigate and provide potential solutions to Data Dictionary, Data Recording and Data Reduction (R2D2) issues to the AEGIS Program Manager. This thesis discusses the history of the R2D2 ACB and its past, present and future directions. Additionally, this thesis examines how the R2D2 ACB concept could be applied to the DD(X) Next Generation Destroyer program. / Civilian, United States Navy

The activity of networks subserving memory and learning in the hippocampus is under the control of GABAergic interneurons. In order to test the contribution of distinct cell types, I have recorded extracellularly, labelled, and identified different types of interneuron in area CA3 of the hippocampus, a region implicated in the generation of gamma and theta oscillations, and the initiation of sharp-waves. I present here a detailed analysis of the spike timing of parvalbumin-positive (PV) basket and physiologically identified pyramidal cells in area CA3, relative to various network states recorded in area CA3 and CA1 simultaneously. Additionally, I have shown by detailed analysis that five classes of previously recorded and identified CA1 interneuron fired with cell type specific firing patterns relative to local gamma oscillations. In CA3, PV basket cells fired phase locked to theta and gamma oscillations recorded in CA1 as well as in CA3, and increased their firing rates during CA1 sharp-waves. Pyramidal cells in CA3 were also phase-locked, but fired at phases different from basket cells. During theta oscillations, CA3 pyramidal and PV basket cells were phase locked to both CA1 and CA3 theta equally, suggesting a wide coherence of these oscillations; in contrast, cells fired more strongly phase-locked to gamma oscillations in CA3 than in CA1, suggesting a specific role for CA3 in the generation of this rhythm. In contrast to theta and gamma oscillations, CA3 basket cells were phase-locked to ripples in area CA3 but not in CA1. Overall, my results show that the spike timing of several types of interneuron in CA1, and PV basket cells in CA3, is correlated in a cell- and area-specific manner with the generation of particular states of synchronous activity.

Ce travail s'inscrit dans le cadre général de l'étude structurale et magnétique de nanomatériaux magnétiques à base de terres rares (R) et de métaux de transition (T) dont le domaine d'application concerne les aimants permanents ou l'enregistrement magnétique à haute densité. Dans la recherche de nouvelles phases aux caractéristiques magnétiques performantes, nous nous sommes intéressés à l'alliage Pr2Co7 . Ce composé a des propriétés magnétiques dures intéressantes : température de Curie élevée et une anisotropie magnéto-cristalline uniaxiale importante. Du point de vue magnétique, les interactions d'échange Co-Co sont les plus fortes, suivies par les interactions R-Co, tandis que les interactions R-R sont très faibles. La dominance des interactions Co-Co induit des températures de Curie assez élevée dans les composés Pr2Co7 . L'anisotropie magnétocristalline résulte de la combinaison de deux anisotropies uniaxiales des réseaux de praséodyme et de cobalt. Afin de renforcer ces interactions, il s'avère nécessaire de substituer partiellement le cobalt dans les composés Pr2Co7 par un élément approprié tel que le fer qui a un rayon légèrement plus gros que celui du cobalt ou par insertion d'un élément léger tel qu’hydrogène ou le carbone qui peuvent augmenter les distances interatomiques et renforcer le moment magnétique. De plus, parallèlement à des propriétés magnétiques intrinsèques performantes, il est nécessaire d'optimiser les propriétés magnétiques extrinsèques de l'alliage par la recherche d'un état nonocristallin convenable correspondant aux applications potentielles. Les propriétés extrinsèques des composés Pr2Co7 n'ont été que très peu étudiées. Plusieurs méthodes ont été utilisées pour l'élaboration des nanomatériaux. Dans notre étude, nous avons mis en œuvre la technique de broyage à haute énergie suivie d'une recristallisation contrôlée, méthode de synthèse qui jusque-là n'avait pas encore été utilisée pour synthétiser ce type de composé. À cette échelle, la taille des grains devient de l'ordre de grandeur de la longueur d'échange. Cette méthode qui constitue un processus de synthèse hors équilibre, permet l'obtention de poudres nanostructurées métastables à partir d'un mélange de poudres élémentaires. Cette technique est particulièrement efficace dans le cas des alliages à base de terre rares (Pr, Sm...) qui sont extrêmement volatil. En effet, elle permet d'éviter l'état liquide puisque la réaction a lieu en dessous de la température de fusion et conduit à des quantités importantes reproductibles et homogènes d'alliages. Nous avons utilisé différentes méthodes de caractérisation à savoir : la diffraction de rayon X (DRX), la microscopie électronique à transmission (MET) couplée avec l'analyse EDX,la Magnéto/susceptométre Manics DSM-8 / This work falls within the general framework of the structural and magnetic nanomaterials based magnetic rare earth (R) and transition metal (T) whose domain of application concerns the permanent magnets or magnetic recording high density. In search of new magnetic phases in performance characteristics, we were interested in the alloy Pr2Co7. This compound has interesting hard magnetic properties: high Curie temperature and uniaxial magnetocrystalline anisotropy important. From the perspective of magnetic exchange interactions Co-Co are the strongest, followed by R-Co interaction, while the RR interactions are very weak. The dominance of Co-Co interactions induces relatively high Curie temperatures in compounds Pr2Co7.The magnetocrystalline anisotropy results from the combination of two networks uniaxial anisotropy praseodymium and cobalt. To strengthen these interactions, it is necessary to partially substitute cobalt in compounds Pr2Co7 by an appropriate element such as iron which has a radius slightly larger than that of cobalt or by insertion of a light element the hydrogen and carbon that can increase the interatomic distances and enhance the magnetic moment. Moreover, along with intrinsic magnetic properties improves, it is necessary to optimize the extrinsic magnetic properties of the alloy by the search for a suitable nanocrystalline state corresponding to the potential applications. The extrinsic properties of compounds Pr2Co7 have been little studied.Several methods were used for the development of nanomaterials. In our study, we have W arranty the technique of high energy milling followed by recrystallization controlled synthesis method which until then had not yet been used to synthesize this type of compound. At this scale, the grain size becomes of the order of magnitude of the exchange length. This method which is a non-equilibrium synthesis process, allows the production of nanostructured powders metastable from a mixture of elemental powders. This technique is particularly effective in the case of alloys based on rare earth (Pr, Sm ...) that are extremely volatile. Indeed, it avoids the liquid state since the reaction takes place below the melting temperature and led to reproducible and large quantities of homogeneous alloys. We used different characterization methods, namely: the X-ray diffraction (XRD), transmission electron microscopy (TEM) coupled with EDX analysis, the magneto / susceptometer Manics DSM-8

Interception and Recording of the Telecommunication Operation Diploma thesis, Hana Valentová, 2013 Abstract This thesis describes legislation of two criminal procedure institutes: the interception and recording of telecommunication operation and the data identification of telecommunication traffic. These institutes are covered in the Code of Criminal Procedure. Purpose of this thesis is to analyze this legislation considering protection of human rights and suggest possible improvements of this legislation, which would prevent inappropriate interventions to human rights, particularly personal privacy. The thesis is composed of introduction, conclusion and five chapters. Introduction explains basic issues of this topic. Primarily it concerns relation between these institutes and human rights and general requirements on such legislation given by The Charter of Fundamental Rights and Freedoms. Subsequently, it describes structure and basic aims of this thesis and secondary sources. Chapter One explains history of development of these institutes. It consists of two subchapters. The first subchapter is concerned with the section 88 of the Code of Criminal Procedure, which governs the interception and recording of telecommunication operation. The second subchapter contains relating institute of the data...

Seguindo uma tendência internacional, o uso de animais no ensino tem sido progressivamente substituído por alternativas, tais como programas de computador, manequins, simuladores, vídeos e atendimento de casos clínicos de rotina. Em consonância com a legislação vigente e em resposta a uma demanda crescente, por parte de alunos e professores, a combinação de tais métodos pode melhor atender aos objetivos de ensino, reduzir custos e evitar o impacto pedagógico e social negativo da experimentação animal. O presente trabalho teve como objetivo propor e avaliar uma alternativa ao método tradicional de ensino de procedimentos com animais de laboratório, através da produção de material didático completo e cientificamente correto, com a finalidade de ser utilizado como parte do programa de ensino da disciplina de Animais de Laboratório do curso de medicina veterinária da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. O material didático demonstrando a execução dos principais procedimentos experimentais em ratos de laboratório foi produzido em mídia digital com filmes e imagens, que por sua vez foi hospedado no portal Boas Práticas em Experimentação Animal (BPEA), localizado no endereço http://www.bpeanimal.iq.usp.br, alberga os vídeos, assim como materiais complementares sobre cada procedimento. Foram capturados os principais procedimentos de manipulação e contenção de ratos de laboratório, assim como as vias de administração de substâncias, sendo elas oral (gavagem), injeções intramuscular, intraperitoneal, intravenosa, subcutânea e intradérmica. Também foram capturadas as vias e técnicas de coleta de sangue, sendo elas: veia lateral da cauda, veia safena, veia gengival e punção intracardíaca. Ainda foi demonstrada a técnica anestésica de bloqueio do nervo ciático. O conteúdo foi disponibilizado para acesso via internet e utilizado como recurso didático na disciplina de Animais de Laboratório. Desta forma, o presente trabalho atende à normativa do CONCEA, que regulamenta o uso de animais no ensino. A metodologia foi muito bem recebida, e evita tanto o estresse desnecessário do estudante quanto do animal durante um procedimento corriqueiro, resultando em melhor assimilação do conteúdo, comprovada pelo bom desempenho dos alunos. Atendendo às pressões econômicas nas universidades, o método proposto representa uma economia financeira substancial em relação ao uso de animais vivos. Conclui-se que este trabalho desenvolveu uma nova metodologia alternativa ao uso de animais vivos em ensino. Todo conteúdo produzido foi postado no portal BPEA, estando já disponível para acesso dos alunos de graduação, com a finalidade de promover conteúdo educacional, proporcionando um legado inédito para o ensino em medicina veterinária no Brasil. / Following an international trend, the animals use in education has been replaced by alternatives such as computer programs, mannequins, simulators, videos and routine clinical case management. According to current legislation and in response to the growing demand from students and professors, alternative methods can better achieve educational objectives, reduce costs, and avoid the negative pedagogical and social impact of animal research. The present study aimed to propose and evaluate an alternative method for the traditional teaching of procedures on laboratory animals. For this purpose, a complete teaching content with scientific basis was produced to be used as part of the Laboratory Animal Science course for the undergraduate students at Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. The content demonstrating how to perform the main experimental procedures on laboratory rats was produced in digital media, including videos and images, which were hosted in Good Practice in Animal Experimentation Portal (BPEA) (http://www.bpeanimal.iq.usp.br). The main handling and restrain techniques of laboratory rats, and drug administration procedures, through different routes, such as oral (gavage), intramuscular, intraperitoneal, intravenous, subcutaneous and intradermal were performed. The blood collection routes and techniques were also recorded, as follows: lateral tail vein, saphenous vein, gingival vein and intracardiac puncture. The sciatic nerve anesthetic blocking technique was also been demonstrated. The content was available online in the BPEA portal, and used as a teaching content for the Laboratory Animals Science course. Thus, the present study complies with the National Council for Animal Experimentation Control (CONCEA) regulations, which regulates animals use in education, resulting in better assimilation of content, as evidenced by the good performance of the students. The method was well received by the veterinary students, and could avoid both students and animals stress during a routine procedure. Attending to the economic pressures of the government in the universities, the method proposed here provides a substantial financial saving when compared to living animals use. In conclusion, this study developed a new alternative methodology for the use of living animals. All content produced was posted in BPEA portal, being available for veterinary students, providing an unprecedented legacy for teaching of veterinary science in Brazil.

Nous avons étudié le couplage dépliement-transport de la Maltose Binding Protein (MBP ou MalE), une protéine périplasmique d'E. Coli et d'un mutant instable, le MalE219, en fonction de la concentration d'un agent dénaturant, le chlorure de guanidium (GdnHCl) à l'échelle de la molécule unique. La technique utilisée est basée sur la détection électrique du transport de macromolécules à travers un nanopore protéique (l'Aérolysine d'Aeromonas Hydrophila) inséré dans une bicouche lipidique plane. Les résultats obtenus ont été comparés à ceux obtenus lors d'une précédente étude réalisée à travers un autre nanopore protéique, l'alpha-hémolysine du Staphylocoque doré, de géométrie et de charge nette différente. Nous avons montré l'existence de temps courts et longs de blocage du courant associés à des protéines dépliées ou partiellement repliées. La fréquence des blocages du courant permet d'obtenir la fraction de protéine dépliée passant à travers le pore en fonction de la concentration en GdnHCl. Les courbes de dénaturation obtenues avec les deux pores montrent un comportement sigmoïdale très similaire. Le type de pore n'influence donc pas la dénaturation des protéines, mais uniquement leur dynamique de transport. En revanche, la courbe de dénaturation du mutant instable présente un déplacement vers les concentrations plus faibles en GdnHCl. Il a été montré également que la présence du maltose comme ligand sur le MalE219 stabilise nettement sa structure. Pour La MBP, les temps de blocages longs diminuent avec l'augmentation de la concentration de GdnHCl montrant une dynamique de transition vitreuse . Cette technique est appropriée à l'étude du dépliement et des changements de conformation de protéines, mais ne permet pas d'obtenir des informations structurales sur les états intermédiaires de repliement. Ainsi, la spectroscopie RMN a été utilisée pour tenter de caractériser ces états intermédiaires de repliement, notamment par la méthode d'échange proton-deutérium. Elle consiste à suivre les cinétiques d'échange des résidus de la protéine sur des spectres 2D 1H-15N HSQC à différentes concentrations de GdnHCl.Ainsi 180 résidus sur les 370 que compte la MBP ont été suivis lors de la dénaturation en présence de GdnHCl. Les deux hélices en C-terminal sont très accessibles au solvant et se dénaturent facilement. La MBP est composée de deux domaines globulaires, le domaine N-ter et le domaine C-ter. Les éléments de structures secondaires situés dans la zone intermédiaire entre les deux domaines (principalement des brins β) sont particulièrement affectés par l'agent dénaturant. D'autres structures secondaires dans les domaines globulaires sont très protégées et plutôt stables. Il est donc proposé que les protéines partiellement dépliées s'insèrent dans le pore par l'extrémité C-terminal et que des parties de structuration tertiaire restent stable entraînant le blocage du pore. / We study the unfolding-transport mechanism of the Maltose Binding Protein (MBP or MalE), a periplasm protein of E. Coli and a destabilised variant, the MalE219, as the function of the concentration of denaturing agent, Guanidine Hydrochloride(GdnHCl) at the single molecule level. The technique is based on the electrical detection of the macromolecule transport through a nanometer-scale channel, Aerolysin channel, inserted into a planar lipid bilayer. Results obtained were compared to previous data with another channel, the alpha-Hemolysin. Both channels have different geometry and net charge.We show that we can distinguish unfolded states from partially folded ones with aerolysin pore.Unfolded proteins induce short current blockades, their duration is constant as a function of the concentration of denaturing agent. Partially folded proteins exhibit long blockades whose life times decrease as the concentration of GdnHCl increase, this indicates a possible glassy dynamics.The frequency of the short current blockades increases as the concentration of denaturing agent increases, following a sigmoidal denaturation curve.The unfolding curve of native MBP with Aerolysin pore is similar to the one previously measured with Hemolysin channel. The denaturation curve of the destabilized variant obtained with Aerolysin is shifted towards lower value of GdnHCl concentration in agreement with bulk measurements. We show also that the addition of maltose stabilizes the structure of MalE219. This nanopore recording technique is also suitable for the study of unfolding and conformation changes of proteins.In order to obtain structural informations that nanopore recording cannot provides, the structure of MBP along its denaturation curve was studied by NMR spectroscopy. The Hydrogen-exchange method known to be sensitive to folding intermediates was specially used. It consists in tracking hydrogen-deuterium exchange rates for amino on the 2D 1H-15N HSQC spectra.Thus, 180 residus of 370 for MBP was followed during denaturation in the presence of GdnHCl. The two last helices in C-terminal of MBP are accessible to the solvent and are denaturated easily. MBP is a two domains protein, N-ter domain and C-ter domain. It was found out that the C- and D-domain of MBP (mainly alpha-helices) could be relatively stable in presence of denaturing agent and that beta strands which make the link between the two domains would be affected by the denaturing agent. It was proposed that partially unfolded proteins enter the pore by the C-terminal end and that stable tertiary structure still present block the pore.

Les années 1945-1960 correspondent en France à un moment important de réoralisation de la poésie : celle-ci est diffusée à la radio, à la télévision, sur disques, dans les cabarets. Les théâtres voient quant à eux se développer, en plus des spectacles poétiques, des tentatives de poèmes scéniques. La « question du livre » et de ses dehors se révèle ainsi centrale dans l’imaginaire et les débats poétiques de cette époque : il s’agit tantôt de repenser les modes de communication du poème, d’ouvrir le domaine poétique au grand public, d’explorer les possibilités offertes par les nouvelles techniques d’enregistrement et de diffusion des sons, tantôt d’user de l’espace médiatique comme d’un lieu de performance poétique. Ce travail, qui rassemble un grand nombre d’archives écrites et audiovisuelles, dresse un état des lieux de ces médiations orales de la poésie de 1945 aux années 1960 en France et propose, à travers l’étude détaillée de diverses formes de « poèmes hors du livre », de réévaluer la place et le rôle de ces pratiques dans l’histoire de la poésie du XXe siècle. / Between 1945 and 1960, French poetry tended to re-explore its oral roots with radio and television broadcasting, recording and live performance. Theatres developed, aside from poetical performances, some attempts for staged poems. The “question of the book” in its relation to other media turned out to be central in the poetical imagination and debates of the time: some endeavoured to rethink the communication modes of poems, to open the poetical field to a wider public, to explore the possibilities offered by new recording and sound broadcasting techniques, others tried to use the media as a poetical performance space. This dissertation, which collects many written and audiovisual archives, surveys the oralisation of French poetry between 1945 and the 1960s and attempts to reassess, through the detailed study of diverse forms of “poems outside the book”, the importance and the role of those practices in twentieth century poetry history.

De março de 2010 a dezembro de 2012, o Observatório de Comunicação, Liberdade de Expressão e Censura (OBCOM) da Universidade de São Paulo (USP) desenvolveu a pesquisa Opinião Pública e Censura no Mundo Contemporâneo. Para abarcar a complexidade e a diversidade de pontos de vista, diferentes instrumentos de investigação foram empregados, dentre eles, a produção de vídeo-entrevistas. Consideramos relevante avivar o debate a incorporação da linguagem audiovisual ao trabalho de pesquisa empírica no âmbito das Ciências Humanas e Sociais tanto para ressaltar quanto justificar possibilidades renovadas de postura, abordagem, interação e divulgação de nossa produção científica. O processo desta proposta audiovisual em seus aspectos epistemológicos, teóricos e técnicos foi o objeto deste trabalho. Partindo da experiência de produção, a metodologia que adotamos converge com experiências contemporâneas da Antropologia, Ciências Sociais, Cinema e Documentário. Sendo assim, a construção da pesquisa colocou em relevo as questões: a) O debate sobre a incorporação das imagens ao trabalho científico e a validade de uma metodologia na pesquisa empírica com audiovisual; b) O questionamento do uso de mídias a partir das Ciências da Comunicação; c) Como o audiovisual foi incorporado em uma pesquisa sobre Opinião Pública e suas possibilidades de análises; d) As renovadas posturas de abordagem, interação e divulgação da produção científica contemporânea. / From March 2010 to December 2012, the Universitys of São Paulo Observatório de Comunicação, Liberdade de Expressão e Censura (OBCOM), developed the research Public Opinion and Censorship in Contemporary World (Opinião Pública e Censura no Mundo Contemporâneo). To arrest the complexity and the diversity of different points of view, multiple investigations apparatus were used, such as the video-recording production. We considered relevant, to enliven the debate, the implantation of audiovisual language in the empiric research work inside Humanities studies. This would justify the renewed possibilities of stance, approach, interaction and disclosing of scientific production and also, would highlight them. The process of this audiovisual proposal in its epistemological, technical and theoretical aspects is the object of this paper. Based on the production experience, we adopt a methodology that converges with contemporary experiences in Anthropology, Social Sciences, Cinema and Documentary. In light of this evidence, the main questions that the research points at are: a-) the debate about the images incorporation in scientific work and the validation of a methodology in empiric research with audiovisual resources; b-) the questioning of media uses in Communication Sciences; c-) how audiovisual resources were used in a research about public opinion and how we can analyze them; d-) the renewed stances of approach, interaction and divulgation of contemporary scientific production.

The Music Industry we knew 20 years ago has evolved into a completely different business. Major labels are scrambling to adapt to a new market created by digital streaming and the heavily dominated mobile environment. The purpose of this study is to explore the choice of remaining independent as a musician, and develop a systematic process that any aspiring artist or band manager can confidently follow to ensure their project has the best chance of success. Most of the published writings on this subject are either too broad, or too business-minded for the typical musician. This thesis provides a creative approach with the organization of the information. I present the process of band management in five chapters named after the five movements of traditional sonata form: The Introduction (Chapter 1) presents the various themes that are covered throughout the study, and describes the reasoning for using sonata form for organization and also elaborates on the author’s background. The Exposition (Chapter 2) describes the beginning stages of creating a band and establishing a creative project in the local music scene. This includes the initial formation of the band and its members, the process of networking within the local music scene, and an in depth explanation of how to effectively utilize all the “Essential Websites.” The Development (Chapter 3) describes the process of developing a band into a consistently gigging1 project. The main topics covered are booking shows, performing shows, recording and releasing an album, and what to focus on after it is released. The Recapitulation (Chapter 4) condenses the main topics of the thesis into a more palatable checklist of essential steps that musicians can easily reference throughout the process of managing their project. The Coda (Chapter 5) looks towards the future of the music industry, and serves as a prediction of how the previously effective methods apply to new technologies and website. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2015. / FAU Electronic Theses and Dissertations Collection

O presente trabalho foi realizado sobre a interpretação da obra para piano Milonga Sureña do compositor argentino Juan José Ramos (1930-1995), tomando como base sua relação com as primeiras milongas campeiras populares de tradição oral de mediados do século XIX, e pesquisando nelas recursos interpretativos aplicáveis à obra mencionada, que são característicos do estilo mas que não são expressos na partitura. Também se escutaram, analisaram e compararam três gravações da obra mencionada, duas em versão original e uma para arranjo de dois violões. Os elementos a analisar nelas foram andamento, dinâmica, articulação e forma, encontrando semelhanças e diferenças entre elas no uso dos mesmos, e também em relação com as milongas tradicionais. / This work deals with the interpretation of the piano work Milonga Sureña of argentine composer Juan Jose Ramos ( 1930-1995 ), based on his relationship with the first popular milongas camperas of oral tradition of the mid-nineteenth century, and researching on them interpretative resources applicable to the work mentioned, which are characteristic of the style but are not specified in the score. Also three recordings of the work were analyzed and compared, two in the original version and one arrenged for two guitars. The analyzed elements were tempo, dynamics, articulation, and form, founding similarities and differences between them and also with the traditional milongas.