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Análises bioquímicas e hsitológicas na micropropagação de abacaxizeiro 'Gomo de Mel' submetido a reguladores vegetais /Fráguas, Chrystiane Borges, 1976- January 2006 (has links)
Orientador: Giuseppina Pace Pereira Lima / Banca: João Domingos Rodrigues / Banca: Moacir Pasqual / Banca: Antonio Natal Gonçalves / Banca: Alessandra Marcondes Feijó Viu / Resumo: Este trabalho teve como objetivo avaliar os efeitos da aplicacao exogena de poliaminas e TIBA (antiauxina) na micropropagacao e anatomia foliar de plantulas de abacaxizeiro eIAC Gomo-de-melf e analisar os teores de poliaminas endogenas, atividade de peroxidase, proteina, IAA-oxidase e a provavel relacao com as diferentes fases da micropropagacao. Inicialmente, retiraram-se as gemas da coroa de frutos sadios e a assepsia foi realizada com hipoclorito de sodio comercial (2 a 2,5% de cloro ativo) a 30% por 20 minutos e lavadas por 3 vezes em agua destilada e autoclavada. Em seguida, inocularam-se as gemas em meio MS solido contendo diferentes combinacoes de BAP (0; 0,5; 1,0 e 1,5 mg L-1) e NAA (0; 0,5 e 1,0 mg L-1). Apos 60 dias, foram selecionados os melhores tratamentos para proliferacao das brotacoes, que foram individualizadas e transferidas para estes meios por mais trinta dias, em meio MS liquido. Plantas oriundas do experimento anterior tiveram as folhas cortadas e apenas segmentos de 1 cm foram inoculados em meio MS liquido contendo os diferentes tratamentos: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPD, T4-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPM, T5-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM PUT, T6-MS + 10 mM SPD, T7-MS + 10 mM SPM e T8-MS + 10 mM PUT. Na ultima fase, segmentos foram inoculados em meio MS liquido contendo os tratamentos: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 0,5 ÊM TIBA, T4-MS + 1,0 ÊM TIBA, T5-MS + 2,0 ÊM TIBA, T6-MS + 4,0 ÊM TIBA, T7-MS + 8,0 ÊM TIBA e T8-MS + 16,0 ÊM TIBA. Apos, as plantas foram aclimatizadas em substrato Plantmax e houve 100% de sobrevivencia, independente do tratamento. Foram realizados cortes histologicos foliares para estudar a influencia das poliaminas e TIBA na anatomia foliar...(Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The effects of exogenous polyamines and TIBA (antiauxin) application in the micropropagation, leaf anatomy of pineapple plants IAC Gomo-de-mel and the contents of endogenous polyamines, peroxidase activity, protein, IAA-oxidase and probable relationship with the different micropropagation phases were studied. Initially, the axillary bud explants were excised from the crown of healthy fruits and the asepsis was accomplished with sodium hypochloride (2 to 2,5% of active chlorine) 30% for 20 minutes and washed 3 times in distilled and autoclaveted water. After, the axillary buds were inoculated in MS solid medium containing the different BAP (0; 0,5; 1,0 and 1,5 mg L-1) and NAA (0; 0,5 and 1,0 mg L-1) combinations. After 60 days, the best treatments were selected for shoot proliferation that were individualized and transferred to these media for more 30 days, in MS liquid medium. Plants from the previous experiment had the leaves cut and just 1 cm segments were inoculated in MS liquid medium containing the different treatments: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPD, T4- MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM SPM, T5-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA + 10 mM PUT, T6-MS + 10 mM SPD, T7-MS + 10 mM SPM and T8-MS + 10 mM PUT. In the last phase, segments were inoculated in MS liquid medium containing the treatments: T1-MS, T2-MS + 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, T3-MS + 0,5 mM TIBA, T4-MS + 1,0 mM TIBA, T5-MS + 2,0 mM TIBA, T6-MS + 4,0 mM TIBA, T7-MS + 8,0 mM TIBA and T8-MS + 16,0 mM TIBA. After, the plants were acclimatizated in Plantmax substrate and there were 100% survival, independent of the treatment. Leaf cuts were made to study polyamines and TIBA influence in the leaf anatomy. The highest shoot number wasobserved with 1,0 mg L-1 BAP + 0,5 mg L-1 NAA, however with hyperhydricity...(Complete abstract click electronic access below) / Doutor
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Regulatory theory (specially regarding the grounds for regulating) / Teoría de la regulación (en especial, acerca de los fundamentos de la regulación)Sacristán, Estela Beatriz 10 April 2018 (has links)
This study analyzes the foundation and the meaning of regulation, specifically from the legal and economic perspective. A basic budget of public interest theories of regulation arises, in a context of freedom. Thus, the role of law as a regulator in society and the various justifications presented for it will be verified. / El presente estudio analiza los fundamentos y el concepto de regulación, vista esta última específicamente desde la perspectiva jurídico- económica. Se propone la existencia de un presupuesto básico de las teorías de interés público de regulación, en un contexto de libertad. De esta manera, se comprobarán la función del derecho como regulador en la sociedad y las diversas justificaciones que se presentan para ello.
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A pós-graduação stricto sensu na forma associativa: o multicêntrico em bioquímica e biologia molecular no campus Governador ValadaresGuimarães, Joicy da Fonseca 25 July 2018 (has links)
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Previous issue date: 2018-07-25 / A presente dissertação é desenvolvida no âmbito do Mestrado Profissional em Gestão e Avaliação da Educação (PPGP) do Centro de Políticas Públicas e Avaliação da Educação da Universidade Federal de Juiz de Fora (CAEd/UFJF). O caso de gestão estudado discutiu os desafios do processo de implementação do Programa Multicêntrico de PósGraduação em Bioquímica e Biologia Molecular na Universidade Federal de Juiz de Fora campus Governador Valadares (PMBqBM/UFJF-GV). Os objetivos definidos para este estudo foram descrever e analisar os processos de regulamentação e de funcionamento do programa e propor um Plano de Ação Educacional para o fortalecimento do PMBqBM em âmbito geral e no campus GV. Assumimos como hipótese a estruturação das responsabilidades e competências nas instâncias de decisão e no âmbito do funcionamento dos cursos como ação necessária ao fortalecimento do programa, o que compreende a melhoria da capacidade de definir e regulamentar as atribuições dos representantes de cada instância e de aperfeiçoar os sistemas de interação e comunicação entre seus membros. Utilizamos como metodologia o estudo de caso e a pesquisa qualitativa e como instrumentos a análise documental e a realização de entrevistas com roteiro semiestruturado. Os resultados nos mostraram que questões relacionadas à promoção da interação nas instâncias de decisão e de atuação, à efetividade da atividade de nucleação e à definição de responsabilidades sobre a gestão e operacionalização de trâmites acadêmico-administrativos merecem atenção. Esses temas são abordados no Plano de Ação Educacional elaborado com vistas ao fortalecimento do PMBqBM como um todo e no campus GV. / The present dissertation was developed on the Professional Master’s Degree in Educational Management and Assessment (PPGP) at the Center for Public Policies and Assessment in Education of the Federal University of Juiz de Fora (CAEd/UFJF). The case study in question discussed the challenges in the process of implementation of the Multicentric Graduate Program in Biochemistry and Molecular Biology at UFJF, Governador Valadares campus (PMBqBM/UFJF-GV). The objectives defined are to describe and analyze the processes of regulating and functioning of the program and to propose an educational action plan to strengthen the PMBqBM as a whole and within the GV campus. We assume as hypothesis the structuring of responsibilities and competences in the decision-making spheres as a necessary course of action to the strengthening of the program. Such conditions comprehend the capacity of defining and regulating the attributions of the representatives of each sphere and the capacity to improve interactional and communicational systems among its members. We have applied the methodology of case study and qualitative research and, as instruments, document analysis and semi structured interviews. The results have shown that the issues related to the promotion of interaction in the instances of decision-making and operationalization of academicadministrative proceedings deserve attention. These themes are dealt with on the education action plan elaborated aiming at the strengthening of the PMBqBM as a whole and within the GV campus.
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Vnímání ekosystémových služeb v městském prostředí / Perception of ecosystem services in an urban environmentNožková, Markéta January 2017 (has links)
This thesis deals with city ecosystem services and how are these services perceived by the visitors of the city nature areas. Specifically, the thesis deals with ecosystem services provided in the city Carlsbad. As the city Carlsbad is the spa city, great deal of visitors of the city nature areas are tourists and that is why are included in the research both city residents and also temporary visitors of the city. From the point of view of the usual division of the ecosystem services, as it was defined by Millennium ecosystem assessment (provisioning services, regulating services, cultural and supporting services), this thesis focuses mainly on the cultural and supporting ecosystem services, because this spectrum of services is presented in the greatest measure in cities in general and these services are overall the most appreciated in the urban areas. As a methodology was chosen a quantitative research, which was conducted by standardized questionnaire consisting of 19 questions. The survey was carried out in electronical form and it took part from November to December 2016. In total, 206 questionnaires were collected. Through the survey was found out, that people are aware of the services provided by nature environment in city as well as the respondents agree, that city nature areas influence the...
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Návrh tepelně-regulačního systému pro přesnou stabilizaci teploty s využitím Peltierových termoelektrických modulů / Design of thermal system for accurate temperature stabilization using Peltier thermoelectric modulesMotl, Jakub January 2011 (has links)
This master’s thesis describes design procedure of a heat-regulating system to stabilize its temperature using a Peltier module. The implementation uses eight-bit microcontroller ATmega16 made by company ATMEL for the local and remote control system. The temperature sensors communicate with the microcontroller by serial SPI interface. To display user data, it has a DEM16217SYH type character LCD display with HD44780 control circuit. To collect data from the cooling areas of Peltier module, accurate ADT7320 temperature sensors are used, made by company Analog Devices. The Control of the Peltier module happens in two points of the electrode system and a bistable hysteresis software regulator is used. The first part summarizes the theory, which concerns with the design. Next part concerned with issues of the Peltier module, overview of temperature sensors, description of the microcontroller, the display unit and the used regulator. Last part describes the hardware and software implementation.
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Samverkan mellan aktörer som arbetar med personer med funktionsnedsättning inom LSSFarzanehfar, Forogh January 2021 (has links)
En person som har insatser enligt LSS (Lagen om stöd och service till vissa funktionshindrade) kan möta många olika aktörer i sin vardag. Studien avsåg att undersöka hur samverkan fungerar mellan aktörerna inom LSS. Frågeställningar behandlade två frågor som handlade om främjande faktorer och hämmande faktorer för samverkan. Urvalet begränsades till personer som arbetar nära brukare och mer eller mindre på daglig basis ser dennes behov av stöd. Verksamhetschef, boendestödjare och platsansvarigchef är de aktörer som har en övergripande bild av samverkan. Utifrån semistrukturerade intervjuer och en tematisk analys genererades 7 teman. Främjande faktorer var gemensam värdegrund, tydlighet och strukturerade rutiner, verksamhetsmål, ömsesidigt samförstånd och förtroende. Hämmande faktorer var lagar och förordningar, brist på kommunikation samt brist på kunskap. Resultatet av undersökningen visar att för en välfungerande samverkan, behövs det samsyn kring aktörernas olikheter. Goda relationer och respekt för varandras uppdrag och regelverk är central i samverkan.
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Implementering och acceptans av skyfallspark i urban miljö : Gestaltningsförslag för två platser i GävleVillagran Ström, Amanda, Waern, Josefine January 2023 (has links)
Det finns flera utmaningar i att kunna hantera klimatförändringar och nederbörd som väntas öka i framtiden. Hårdgjorda ytor och fortsatt förtätning av urbana områden försvårar samt ställer högre krav på städers dagvattenhantering. Ytor som idag har andra funktioner behöver därför anpassas för att kunna fylla så många funktioner som möjligt på grund av det begränsade utrymmet i många städer. Multifunktionella ytor kan bland annat tillhandahålla grön och blå infrastruktur i kombination med lek och rekreation. Forskning och studier visar att det finns många sätt som grön och blå infrastruktur kan implementeras i staden. Kombinationen av kostnader, estetik, funktion och vad människor i omgivningen tycker går emellertid inte alltid hand i hand. Studien genomfördes i centrala Gävle. Målet med studien är att visa hur ett gestaltningsförslag kan användas för att öka acceptansen vid planering och genomförande när skyfallsparker implementeras i befintlig eller ny park och lekplats. Syftet med studien var att undersöka hur skyfallsparker kan införas i urbana parker samt lekplatser utan att påverka den mänskliga användningen av platsen negativt. För att möta syftet genomfördes sju metoder som validerade varandra. En skyfallskartering genomfördes först för att lokalisera två platser med översvämningsrisk som gestaltningsförslag skulle tas fram för, vilket blev lekplatsen Apelgatan samt Skogsfruparken. För att skapa en ökad kunskap om hur platser kan utformas för att kunna omhänderta vatten vid regn och skyfall genomfördes djupintervjuer, platsobservationer samt platsbesök i Uppsala och Stockholm. Platsobservationerna visade även tillsammans med intervjuer i fält hur platserna används i dagsläget och vad på platsen som uppskattas av användarna. Gestaltningsförslag för båda platserna utformades för att möjliggöra för både lek och rekreation samt med en ökad kapacitet omhänderta vatten. Slutligen validerade enkätsvaren om förslagen skulle påverka respondenternas användning av platsen och om förändringen accepterades av användarna. Resultatet visar att det går att utforma skyfallshantering på flera sätt, där dagvattendamm är ett. Genom att undersöka acceptansen för de två framtagna gestaltningsförslagen gick det att bedöma att en skyfallspark bland annat kan utformas som studiens förslag för att bli accepterad av användarna. Valet av att validera med en enkät var tillräckligt för att visa på acceptansen i denna studie eftersom den grundar sig på flera metoder. Enkäten kunde dock ha utformats på ett annat sätt för att ge respondenterna en bättre bild av gestaltningarna, eller ha kompletterats med en fokusgrupp för att fördjupa sig ytterligare i acceptansen. / There are several challenges in managing climate change and precipitation, which is expected to increase in the future. Impermeable surfaces and continued densification of urban areas makes it more difficult and set higher demands on urban stormwater management. Because cities have limited space, there is a need for areas that today have other functions to be adapted and to be able to fulfil as many functions as possible. Multifunctional spaces in the city can provide blue-green infrastructures in combination with play and recreation. Research and studies show that there are many ways that green and blue infrastructure can be implemented in the city, but the combination of costs, aesthetics, functions, and what people in the surroundings think do not always go hand in hand. The study was conducted in central Gävle. The goal of the study is to show how a design proposal can be used to increase acceptance during planning and implementation, when rain parks are implemented in existing or new parks and playgrounds. The purpose of the study was to investigate how stormwater management can be implemented in urban parks and playgrounds without negatively affecting the human use of the place. Seven methods, which validated each other, were implemented to meet the purpose. A cloudburst mapping was first carried out to locate which two places have risk of flooding, for which design proposals should be made. This resulted in the playground Apelgatan and the park Skogsfruparken. Interviews, site observations and site visits in Uppsala and Stockholm, contributed to an increased knowledge of how places can be designed to manage rain and cloudburst. The site observations showed, with interviews in the field, how the sites are currently used and what elements were appreciated by users. Design proposals for both sites were then designed to allow both play as well as to increase capacity to accumulate water. A survey validated whether the proposals would affect respondents' use of the site and whether the change were accepted by the users. The result shows that it is possible to design stormwater management in several ways, of which stormwater ponds are one. By investigating the acceptance of the two design proposals, it was possible to assess that stormwater management can for instance be designed as the study's proposal to be accepted by the users. The choice of a survey was sufficient to show the acceptability in this study as it is based on several methods. However, the survey could have been designed in a different way to give the respondents a better overall picture of the visualisations or could have been supplemented with a focus group to delve further into acceptance.
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The South African regulatory framework relating to illegal trade in rhino horn / Neil James de WetDe Wet, Neil James January 2015 (has links)
The purpose of this study is to determine whether the current South African regulatory framework related to the illegal trade in wildlife, provides the means to regulate the illegal trade in rhino horn effectively. In an effort to combat and eradicate the illegal trade in wild species and parts therein, South Africa has enacted numerous laws and it has ratified various international conservation Conventions. However, with more than 800 rhinos having been killed in 2013 alone, it is doubtful whether these laws and conventions are adequate.
The first chapter is an introductory chapter summarising the latest statistics on rhinos fatalities as a result of poaching, an estimate on their numbers as well giving a brief overview of what laws and conventions will be discussed in this study. In the second chapter the historical development of the trade in rhino horn is dissected, who the culprits are and the reason why rhinos are poached for their horn. The third chapter analyses international conventions that have been adopted by South Africa, including conventions such as the Convention on International Trade in Enangered Species, the Convention on Biological Diversity and agreements such as the General Agreement on Tariffs and Trade. The fourth chapter will focus more on the regional contributions which the African Union and Southern African Developing Community have made in an effort to combat the illegal trade in endangered species and their products such as rhino. The fifth and final chapter discusses South African legislation which comprises of the National Environmental Management Act, National Environmental Management Biodiversity Act, National Environmental Management Protected Areas Act and the Criminal Procedure Act so as to determine what influence it has had in the effort to regulate the illegal trade in rhino horn.
The conclusion that can be drawn from this study is that the South African regulatory framework on paper is indeed adequate enough to regulate and even eradicate the illegal trade in rhino horn. The reason, however, for it’s currently failure is attributable to a lack of enforcement of these measures as a result of a lack of provisions holding entities accountable. / LLM (Import and Export Law), North-West University, Potchefstroom Campus, 2015
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The South African regulatory framework relating to illegal trade in rhino horn / Neil James de WetDe Wet, Neil James January 2015 (has links)
The purpose of this study is to determine whether the current South African regulatory framework related to the illegal trade in wildlife, provides the means to regulate the illegal trade in rhino horn effectively. In an effort to combat and eradicate the illegal trade in wild species and parts therein, South Africa has enacted numerous laws and it has ratified various international conservation Conventions. However, with more than 800 rhinos having been killed in 2013 alone, it is doubtful whether these laws and conventions are adequate.
The first chapter is an introductory chapter summarising the latest statistics on rhinos fatalities as a result of poaching, an estimate on their numbers as well giving a brief overview of what laws and conventions will be discussed in this study. In the second chapter the historical development of the trade in rhino horn is dissected, who the culprits are and the reason why rhinos are poached for their horn. The third chapter analyses international conventions that have been adopted by South Africa, including conventions such as the Convention on International Trade in Enangered Species, the Convention on Biological Diversity and agreements such as the General Agreement on Tariffs and Trade. The fourth chapter will focus more on the regional contributions which the African Union and Southern African Developing Community have made in an effort to combat the illegal trade in endangered species and their products such as rhino. The fifth and final chapter discusses South African legislation which comprises of the National Environmental Management Act, National Environmental Management Biodiversity Act, National Environmental Management Protected Areas Act and the Criminal Procedure Act so as to determine what influence it has had in the effort to regulate the illegal trade in rhino horn.
The conclusion that can be drawn from this study is that the South African regulatory framework on paper is indeed adequate enough to regulate and even eradicate the illegal trade in rhino horn. The reason, however, for it’s currently failure is attributable to a lack of enforcement of these measures as a result of a lack of provisions holding entities accountable. / LLM (Import and Export Law), North-West University, Potchefstroom Campus, 2015
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Organisatoriska förutsättningar för implementering av ekosystemtjänster i stadsplanering : - En kvalitativ studie av Nacka kommunPersson, Amanda, Stikå, Maria January 2016 (has links)
Denna studie syftar till att synliggöra och utforska vilka möjligheter och utmaningar som finns vid implementering av ekosystemtjänster istadsplanering. Studien bygger på Nacka kommuns arbete med ekosystemtjänster. Empiriskt har en intervjustudie genomförts med berördatjänstemän och ledande politiker kring kommunens praktiska arbete med att förhålla sig till och använda ekosystemtjänster i stadsplanering. Detempiriska materialet analyseras och sätt i relation till tidigare forskning inom frågan. Resultatet visar att det finns både möjligheter ochutmaningar i Nacka kommun med att implementera ekosystemtjänster i stadsplanering. Det framkommer att kommunikation, samverkan ochkunskap är tre viktiga faktorer för en lyckad implementering. För att kommunen ska ha en långsiktig stadsplanering där ekosystemtjänster spelaren viktig roll, krävs en kontinuerlig dialog mellan tjänstemän och politiker, liksom politisk enighet. Ett framträdande projekt är Grönytefaktornsom innefattar mestadels möjligheter med att integrera ekosystemtjänster i stadsplanering.
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