*‘Pasja’ (Brassica campestris x napus) and kale (Brassica oleracea var. acephala L.) were grown at Lincoln, Canterbury, New Zealand in 2008 with different levels of phosphorus (P) fertiliser. Banded or broadcast P fertiliser was applied at 0, 20, 40 and 60 kg P/ha at establishment. Total dry matter (DM) production, the proportion of the leaf and stem and leaf area development were measured over time and related to the biophysical environment. For ‘Pasja’, final DM increased with P rate from 3730 kg DM/ha to ~4900 kg DM/ha at 60 kg P/ha. For kale the increase was from 8710 kg DM/ha for the control to ~11000 kg DM/ha for all P treatments. The leaf to stem ratio declined from 22-31 at 17 days after emergence to 10.4 at the final harvest for ‘Pasja’, which meant the crop was effectively made up mainly of leaf (~90%). The ratio for kale declined from 2.7 at 24 days after emergence to 0.64 at the final harvest. The leaf to stem ratio for both species did not respond to either the method of application or rate of P. Seedling DM accumulation increased with applied P over the first 10 to 17 DAE for ‘Pasja’ and kale respectively. The crops went from shoot growth priority to root growth. The phyllochron of both species was unaffected by P application but responded linearly to the temperature above 0°C. For ‘Pasja’ the phyllochron was 60°Cd compared with 109°Cd for kale. As a consequence ‘Pasja’ developed its canopy and reached critical leaf area index (LAIcrit) earlier than kale. Leaf area index (LAI) for the control crops of both species was lower than for P fertiliser treatments with a maximum of 3.6 for ‘Pasja’ and 3.8 for kale. There was no difference in leaf area indices among the P fertiliser treatments for ‘Pasja’, while kale LAI differed with the rate of P application up to 40 kg P/ha. Total accumulated intercepted solar radiation (RIcum) was 8 and 11% greater for ‘Pasja’ and kale crops respectively when P was applied compared with the control. Thus, the difference in total dry matter yield due to P application was attributed to the difference in RIcum. Neither the method of application or rate of P applied affected the radiation use efficiency (RUE) of either crop. For ‘Pasja’ the RUE was 1.1 g DM/MJ PAR and for kale 1.33 g DM/MJ PAR. Based on this research, it was concluded that P application increased RIcum as a result of increased LAI. The difference in total DM yield was attributed to differences in RIcum. It is recommended that farmers growing ‘Pasja’ and kale under similar conditions to this experiment should apply 40 kg P/ha for ‘Pasja’ and band 20 kg P/ha for kale. *‘Pasja’ is considered both as a species and cultivar in this document as it marketed as such in New Zealand. Technically ‘Pasja’ is a leaf turnip.

NEVES, Antônia Leila Rocha. Irrigation of cowpea with saline water at different growth stages and their effects on the plant and soil. 2008. 119 f. : Dissertação (mestrado) - Universidade Federal do Ceará, Centro de Ciências Agrárias, Departamento de Engenharia Agrícola. 2008 / Submitted by demia Maia (demiamlm@gmail.com) on 2016-06-13T14:53:10Z No. of bitstreams: 1 2008_dis_alrneves.pdf: 903212 bytes, checksum: 4298d42aacb4a0815d2c06c72d764de8 (MD5) / Approved for entry into archive by demia Maia (demiamlm@gmail.com) on 2016-06-13T14:57:22Z (GMT) No. of bitstreams: 1 2008_dis_alrneves.pdf: 903212 bytes, checksum: 4298d42aacb4a0815d2c06c72d764de8 (MD5) / Made available in DSpace on 2016-06-13T14:57:22Z (GMT). No. of bitstreams: 1 2008_dis_alrneves.pdf: 903212 bytes, checksum: 4298d42aacb4a0815d2c06c72d764de8 (MD5) Previous issue date: 2008 / The sensitivity of crops to salinity often changes from one growth stage to another. Cowpea [Vigna unguiculata (L.) Walp.] is an important crop specie cultivated in different semi-arid regions of the world. The objective of this work was to evaluate the effect of the irrigation with saline water, applied at different growth development stages of cowpea. The experiment was set up in the field, during the dry season. A completely randomized block design, with five treatments (T1, T2, T3, T4 and T5) and five repetitions, was adopted. The distance between lines and plants were 0.8 m and 0.3 m, respectively, with two plants per hole. The treatments studied were: T1 - Groundwater with electrical conductivity (ECw) of 0.8 dS m-1 during the whole crop cycle; T2 - Saline water (ECw = 5.0 dS m-1) during the whole crop cycle, starting after germination; T3 - Saline water (ECw = 5.0 dS m-1) from sowing until the 22th day after sowing (germination and initial growth) and groundwater in remaining stages of the crop cycle; T4 - Saline water (ECw = 5.0 dS m-1) from the 23th to the 42nd day after germination (intense growth and pre-flowering), and groundwater irrigation for the remaining growth stages. T5 - Groundwater from sowing to the 42nd day after sowing and saline water (ECw = 5.0 dS m-1) during flowering and pod-filling stages. Soil coverage, gas exchange, vegetative growth and crop yield were measured. The mineral concentration and quantity of nutrient removed from the soil, as well as the nutrient use efficiency and salt accumulation in soil were also determined. The irrigation with combination of groundwater and saline water reduced the salt accumulation in soil, in relation to continuous use of saline water. The continuous application of water with ECw of 5.0 dS m-1 (T2) reduced the rates of photosynthesis and transpiration, in relation to the well water (T1). Plants of the treatment 3 showed reduction in gas exchanges only in the first measurement, while the saline water application in other growth stages (T 4 and T5) did not provoke significant reductions in leaf in the gas exchanges of the plants. It was found that the continuous use of saline water inhibits plant growth, while the irrigation with saline water during germination and initial growth causes retardation in plant development. For treatments 2 and 3, reduction in the number of pods and in seed production was verified. Irrigation with saline water from the 23rd to the 42nd day (T4) and from the 43rd to the 63rd (T5) day after sowing did not affect reproductive and vegetative growth, but the saline water application in the pre-flowering (T4) caused anticipation of reproductive cycle. Cowpea plants removed the minerals analyzed in the following decreasing sequence: N > K > Cl > Ca > Na > P > Fe > Zn > Mn > Cu, but the continuous use of saline water (T2) reduced the total extracted for the most nutrients, except for Na e Cl. The minerals Na, Cl, K, Ca, Fe and Mn were distributed preferentially to vegetative plant parts, while the most of N and P were exported in the pods. The irrigation with saline water during germination and initial growth stage (T3) reduced the mineral use efficiency of most of analyzed nutrients. The continuous application of saline water (T2) reduced the agronomic efficiency of the fertilizer application (N, P and K), but it did not affect the nutrient use efficiency by the plants. / A sensibilidade à salinidade varia com o estádio de desenvolvimento da cultura. O feijão-de-corda [Vigna unguiculata (L.) Walp.] constitui uma das principais culturas, em diversas regiões semi-áridas do mundo. Avaliou-se o efeito da irrigação com água salina, aplicada em diferentes estádios de desenvolvimento de plantas de feijão-de-corda. O experimento foi conduzido no campo, e obedeceu ao delineamento em blocos ao acaso, com cinco tratamentos (T1, T2, T3, T4 e T5) e cinco repetições. O feijão-de-corda foi cultivado no espaçamento de 0,8 m entre linhas e 0,3 m entre plantas, com duas plantas por cova. Os tratamentos utilizados foram: T1 - plantas irrigadas com água do poço (CEa de 0,8 dS m-1) durante todo o ciclo; T2 - Água salina com CEa de 5,0 dS m-1, com aplicação iniciada após a germinação e permanecendo até o final do ciclo; T3 - Água salina com CEa de 5,0 dS m-1, da semeadura até 22 dias após o plantio (DAP), correspondendo às fases de germinação e crescimento inicial, e água do poço no restante do ciclo; T4 - Água salina com CEa de 5,0 dS m-1, aplicada de 23 a 42 DAP (fase de intenso crescimento vegetativo até a pré-floração), e água do poço nas demais fases do ciclo; T5 - Água do poço da semeadura até 42 DAP e água salina (CEa de 5,0 dS m- 1) aplicada a partir dos 43 DAP (floração e frutificação). Foram avaliadas a cobertura vegetal do solo, crescimento vegetativo, fotossíntese, transpiração, condutância estomática, produtividade, partição de matéria seca, os teores, os totais extraídos e a distribuição dos nutrientes na planta, a eficiência nutricional, eficiência no uso da água e o acúmulo de sais no solo. A irrigação com a combinação de água do poço com água salina reduziu o acúmulo de sais no solo, em relação ao T2. O T2 reduziu as taxas de fotossíntese e transpiração, em relação ao T1. O T3 provocou redução das trocas gasosas somente na primeira medição, enquanto, que similar aplicação nos outros estádios (T4 e T5) não provocou reduções significativas nas trocas gasosas das plantas. O T2 inibiu o crescimento vegetativo da planta, enquanto o T3 provocou retardo no desenvolvimento da planta. Os tratamentos T2 e T3 provocaram reduções significativas no número de vagens e na produção de sementes por planta, em relação aos demais tratamentos. Por outro lado, os tratamentos T4 e T5 não afetaram o crescimento e a produtividade da cultura, sendo que o T4 causou aceleração no ciclo reprodutivo da cultura. Os minerais foram extraídos pelo feijão-de-corda na seguinte ordem decrescente: N > K > Cl > Ca > Na > P > Fe > Zn > Mn > Cu, no entanto, o T2 reduziu a extração da maioria dos elementos analisados, com exceção do Na e Cl. Os minerais Na, Cl, K, Ca, Fe e Mn permaneceram preferencialmente nas partes vegetativas enquanto N e P foram exportados em maiores proporções pelos frutos. O T3 reduziu a eficiência de utilização da maioria dos nutrientes. O T2 reduziu a eficiência agronômica de utilização de N, P e K, entretanto não afetou a eficiência de utilização dos nutrientes extraídos pelas plantas.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The common bean (Phaseolus vulgaris L.) is an important calcium source for human diet, thus the evaluation of the calcium use efficiency in common bean a relevant tool for crop sustainability. The aims of this study were to evaluate the response in common bean cultivars with different calcium concentrations in nutrient solution on dry matter production, yield components and calcium, potassium and magnesium accumulations; to investigate the association between these characters; to evaluate the genetic variability of lines for calcium availability in the solution; and identify lines of common bean-efficient and responsive to calcium application by different indexes. For this, three experiments were conducted from March 2011 to July 2012 in greenhouse, in a completely randomized design, in splitplots. In the first two experiments were tested calcium concentrations in nutrient solution from 1.10 to 4.95 mmol L-1 as components of the main plot and the BRS Expedito, Carioca and Iraí cultivars on subplots in the first experiment, with the exclusion of the cultivar Iraí in the second experiment. The third experiment evaluated the efficiency and the response of the calcium use of 12 different common bean lines at low (1.10 mmol L-1) and high (3.85 mmol L-1) calcium concentrations in the nutrient solution. Higher values for dry matter and grain yield components were observed for 3.30 mmol L-1 and 4.95 mmol L-1 calcium concentrations in the solution. The common bean cultivars acumulate greater quantity of calcium in the leaves, followed by stems, pods and grains. Highest accumulation of calcium in the leaves was obtained at a 4.95 mmol L-1 calcium concentration in the nutrient solution, and the calcium concentration in the leaves was 21 times higher than the value obtained in the bean grains. The increase of calcium concentration in the nutrient solution up to the concentration of 4.95 mmol L-1 does not change the absorption and the accumulation of potassium and magnesium in leaves and grains. Indirect selection by greater dry matter and higher values of the variables that form the grain yield in common bean will be efficient to increase the accumulation of calcium in grains. The lines L 15, L 234, L 246 and L 77 respond to the increase on calcium supply in the solution, because more calcium accumulates in grain in the cultivation with high calcium concentration. The lines L 246 and L 15 are calcium use efficient in the plant, in the acquisition and grain production, and are not responsive to calcium application. The line L 77 is calcium use efficient in the plant and in the grain production and is responsive to the calcium application in the nutrient solution. / O feijão (Phaseolus vulgaris L.) é uma importante fonte de cálcio para a alimentação humana, sendo a avaliação da eficiência no uso de cálcio em feijão uma ferramenta relevante para a sustentabilidade da cultura. Foram objetivos desse trabalho avaliar a resposta de cultivares de feijão a diferentes concentrações de cálcio em solução nutritiva na produção de massa seca, nos componentes da produtividade de grãos e no acúmulo de cálcio, potássio e magnésio; investigar a associação entre esses caracteres; avaliar a variabilidade genética de linhagens de feijão quanto a disponibilidade de cálcio na solução e identificar linhagens eficientes no uso de cálcio e responsivas a aplicação deste mineral por diferentes índices. Para isso foram realizados três experimentos no período de março de 2011 a julho de 2012, em casa-devegetação, em delineamento inteiramente casualizado, com parcelas subdivididas. Nos dois primeiros experimentos foram testadas concentrações de cálcio na solução nutritiva de 1,10 a 4,95 mmol L-1 como componentes da parcela principal e as cultivares BRS Expedito, Carioca e Iraí nas subparcelas no primeiro experimento, com a exclusão da cultivar Iraí para o segundo experimento. No terceiro experimento foram avaliadas a eficiência no uso e a resposta de 12 linhagens de feijão em baixa (1,10 mmol L-1) e em alta (3,85 mmol L-1) concentração de cálcio na solução nutritiva. Maiores valores para massa seca e para os componentes da produtividade de grãos foram observados para as concentrações de 3,30 mmol L-1 e de 4,95 mmol L-1. As cultivares de feijão acumulam maior quantidade de cálcio nas folhas, seguido pelos caules, vagens e grãos. Maior acúmulo de cálcio nas folhas foi obtido na concentração de 4,95 mmol L-1 de cálcio na solução nutritiva, quando a concentração de cálcio nas folhas é 21 vezes superior ao valor obtido nos grãos de feijão. O aumento da concentração de cálcio na solução nutritiva até a concentração de 4,95 mmol L-1 não altera a absorção e o acúmulo de potássio e de magnésio nas folhas e nos grãos de feijão. A seleção indireta pela maior massa seca de parte aérea e maiores valores das variáveis que formam a produtividade de grãos em feijão será eficiente para aumentar o acúmulo de cálcio nos grãos de feijão. As linhagens L 15, L 234, L 246 e L 77 respondem ao aumento do suprimento de cálcio na solução, pois acumulam mais cálcio nos grãos em cultivo com alta concentração deste mineral. As linhagens L 246 e L 15 são eficientes no uso de cálcio na planta, na aquisição de cálcio e na produção de grãos, e não são responsivas à aplicação de cálcio. A linhagem L 77 é eficiente no uso de cálcio na planta e na produção de grãos e é responsiva à aplicação de cálcio na solução nutritiva.

The Penman-Monteith (PM) equation is a state-of-the-art modelling approach to simulate evapotranspiration (ET) at site and local scale. However, its practical application is often restricted by the availability and quality of required parameters. One of these parameters is the canopy conductance. Long term measurements of evapotranspiration by the eddy-covariance method provide an improved data basis to determine this parameter by inverse modelling. Because this approach may also include evaporation from the soil, not only the ‘actual’ canopy conductance but the whole surface conductance (gc) is addressed. Two full cycles of crop rotation with five different crop types (winter barley, winter rape seed, winter wheat, silage maize, and spring barley) have been continuously monitored for 10 years. These data form the basis for this study. As estimates of gc are obtained on basis of measurements, we investigated the impact of measurements uncertainties on obtained values of gc. Here, two different foci were inspected more in detail. Firstly, the effect of the energy balance closure gap (EBCG) on obtained values of gc was analysed. Secondly, the common hydrological practice to use vegetation height (hc) to determine the period of highest plant activity (i.e., times with maximum gc concerning CO2-exchange and transpiration) was critically reviewed. The results showed that hc and gc do only agree at the beginning of the growing season but increasingly differ during the rest of the growing season. Thus, the utilisation of hc as a proxy to assess maximum gc (gc,max) can lead to inaccurate estimates of gc,max which in turn can cause serious shortcomings in simulated ET. The light use efficiency (LUE) is superior to hc as a proxy to determine periods with maximum gc. Based on this proxy, crop specific estimates of gc,maxcould be determined for the first (and the second) cycle of crop rotation: winter barley, 19.2 mm s−1 (16.0 mm s−1); winter rape seed, 12.3 mm s−1 (13.1 mm s−1); winter wheat, 16.5 mm s−1 (11.2 mm s−1); silage maize, 7.4 mm s−1 (8.5 mm s−1); and spring barley, 7.0 mm s−1 (6.2 mm s−1).

MESHWR / Department of Hydrology and Water Resources / Irrigation uses the largest amount of water, estimating to 60 % of the total consumption in South Africa. For this reason, the efficient and reasonable use of water by irrigators is of paramount importance. Thus, this study was carried out to assess the suitability of Wetting Front Detectors (WFDs) in improving water management. The study involved an on-farm survey; field installations; testing of WFD technology on selected plots within the scheme; identification of the crops grown; documentation of the current water supply and documentation of the challenges faced by farmers in relation to irrigation. These were carried out to identify the ideal situations in the scheme. Irrigation scheduling helps farmers to know when to irrigate and amount of water required supplying for crop need. The study presented WFD, as a means of improving irrigation efficiency. The WFD is a simple tool that helps farmers to identify what is occurring around the root zone. Four plots with a representative farmer in each of the plot were identified in four Blocks (Block 1 farmer 1, Block 1 farmer 2; Block 2 farmer 1; Block 3 farmer 1 and Block 4 farmer 1). On-farm experiment of the WFD was carried out. However, with Block 4 farmer 1, insufficient data was collected due to absence of LongStop equipment. This also involved field installation, observation and measurements of the LongStops (LSs) and FullStops (FSs) WFDs at placement depth of 30 cm, 45 cm and 60 cm. The efficiency of an irrigation system depends on different performance indicators including Irrigation Efficiency (IE), Conveyance Efficiency (CE), Application Efficiency (AE), Storage Efficiency (SE), Distribution Uniformity (DU) and Coefficient uniformity (CU). In this study, attention was focused only on DU; CU and SE, as represented by water moisture availability. All the DU for all plots in blocks were below the standard DU of furrow, which is 65%. Farmer 2, in Block 1, had a higher DU and CU, which were 60% and 68%, respectively- considered closer to the standard DU value. For the other farmers, their DU and CU prior to irrigation were very low, which indicated that there was uneven distribution of water in these plots. The poor DU in Block 1 farmer 1, indicated by the uneven infiltrated water, resulted in excessive watering. Analysing the WFD showed that farmers were performed well in all the Blocks, except for farmer 1 in Block 1. Average soil moisture content result indicated high water loss through deep percolation. The highest volumes of water recorded before and after irrigation were 131 ml and 159 ml, respectively, for LS90 placed at a depth of 90 cm in Block 2 farmer 1. High volumes of water were collected in Block 1 farmer 2, Block 2 farmer 1 and Block 3 farmer 1 before and after irrigation. The result showed that, the more placement depth down the soil profile, the more accumulation of water in the LSs. Therefore, it was recommended that farmers continue to use the WFD as a tool for irrigation efficiency. However, there is need for improvement and capacity building in using the tool.

Text in English with abstracts in English, Tsonga and Sepedi and keywords in English / This study was carried out to investigate the performance and quality of Cucumis metuliferus E. Mey. Ex Naudin (African horned cucumber) under protected and open environment with the aim of comparing yield and quality for the purpose of commercialisation of the crop. Therefore, the overall objective was to determine a suitable growing environment for C. metuliferus between greenhouse, shade net and open field, so that a comparative yield and quality analysis could be done for the purpose of commercialisation of the crop. Cucumis metuliferus seeds are difficult to germinate under the normal suitable environmental conditions in which most farmers operate. Germination was evaluated with respect to growth medium, scarification and seed certification. These factors ultimately control yield and fruit quality. The main aim of the study was to investigate the effect or impact of seed certification, growth medium (sand and vermiculite, peat TS1 and seedling mix) and scarification on germination success of C. metuliferus seeds. The seeds were classified under four different categories viz. treated certified, non-treated certified, treated uncertified, and non-treated uncertified. Of the 540 certified and uncertified seeds sown in the three-growth media, 80% germinated, significantly more that those that failed. The treatment combination of treated certified seeds (TC) and peat demonstrated high germination success rate of 93.6%, followed by the treatment combination of treated certified seeds (TC) and sand+ vermiculite with germination success rate of 91.3%. The treatment combination of uncertified untreated (UTU) seeds and potting mix illustrated low germination success rate at 37.2%. In general, the study results revealed that certified seeds scarified with warm water combined had a higher germination rate that unscarified seeds, irrespective of the growth media. Since the seedling root-ball integrity is essential for transplant survival, this study suggests peat and certified seeds as the best combination for propagation and good quality plants. Water scarcity, population growth and climate change are the major factors affecting agricultural productivity in the 20th century. Cucumis metuliferus grows naturally in the wild; however, its yield response to water stress, different cultivation environment and soil types, has not been assessed. A study was carried out to determine water use efficiency of the C. metuliferus grown the greenhouse, shade net and open field under varying soil types and irrigation water levels, so that a comparative analysis could be done on productivity levels. The research was conducted at the University of South Africa (Unisa) Science Campus, in Florida, Gauteng (-26.157831 S, 27.903364 E) during the 2017/2018 and 2018/2019 growing seasons. A factorial experiment with two factors – soil (loamy soil and sandy loam soil) and water stress levels (no water stress, moderate water stress and severe water stress). The pot experiments were a completely randomised design with nine (9) replicates per treatment. Data collected included total biomass, aboveground biomass, harvest index and water use efficiency. Results illustrated that treatment of moderate water stress combined with loamy soil and shade net decreased WUE from 6.2 to 1.4 kg m-3, whereas treatment combination of no water stress combined with sandy loam and open field environment demonstrated increase in WUE from 1.4 to 6.2 kg m-3. Nutritional concentration of most crops depends on factors such as amount of water, growing environment, light intensity and soil types. However, factors influencing nutritional concentration of C. metuliferus fruits is not yet known. Another objective of the study was to determine the effect of different water stress levels, soil types and growing environment (greenhouse, shade net and open field) on the concentration of nutrients in C. metuliferus fruit. Freeze-dried fruit samples were used in the quantification of ꞵ-carotene, vitamin C, vitamin E, total soluble sugars, crude proteins, total flavonoids, total phenols, macro-nutrients (Ca, Mg, P, K, Na and S), and micro-nutrients (Cu, Fe, Mn and Zn). Results demonstrated that plants grown under shade net, combined with severe water stress level and loamy soil, had increased total soluble sugars (15.8 ˚Brix) compared to other treatments. Plants under shade net environment, combined with moderate water level and loamy soil, resulted in increased crude protein content (6.31 ˚Brix). The severe water stress treatment combined with loamy soil under greenhouse conditions resulted in increased ꞵ-carotene content (1.65 mg 100 g-1 DW) when compared to other treatments. Regarding vitamin C, the treatment of no water stress combined with loamy soil under shade net environment showed higher content of (33.1 mg 100 g-1 DW). The severe water stress treatment combined with sandy loam soil under greenhouse environment, increased vitamin E content (35.1 mg 100 g-1 DW) when compared to other treatments. The treatment of open field under severe water stress level and loamy soil increased total flavonoids content (0.85 mg CE/g-1 DW) in the fruit when compared to other treatments. The results thus imply that this plant bears better-quality fruit in terms of concentration of nutrients and biochemical constituents when grown under no to moderate water stress treatment on the loamy or sandy loam substrate in the shade net and open field environment. Primary metabolites are biological compounds that are essential to the growth and development of a plant during its life cycle. They have a direct impact on the yield and biochemical constituents in plants. Quantities of the primary metabolites were determined using the LC-MS-8040 triple quadrupole mass spectrometer (Shimadzu) from fruits harvested from treatments mentioned above. The results showed that the no water stress treatment combined with sandy loam under shade net environment significantly (P≤0.05) increased asparagine content from 10×106 to 80x106 peak intensity when compared to other treatments. The severe water stress treatment combined with sandy loam soil under open field environment during the 2017/2018 season, significantly increased dopa content from 12,030 to 324,240 peak intensity, while during the 2018/2019 season, 4-hydroxyproline from 10×106 to 90x106 peak intensity the was significantly increased. The study suggests that the treatment combination of water stress levels (no water stress and severe water stress) and soil substrates (loamy soil and sandy loam) under greenhouse and shade net significantly affected the shift of primary metabolites profile of C. metuliferus fruit as opposed to individual factors, respectively. There is therefore great potential to commercialise this crop; however, there is still a great deal that is not well understood of its growth habits and biological/biochemical constituents as a future alternative crop. / Ndzavisiso lowu wu endliwe ku lavisisa hi matirhelo na khwaliti ya Cucumis metuliferus E. Mey. Ex Naudin (African horned cucumber) eka mimbangu na mavala lama sirheleriweke na hi xikongomelo xa ku kotlanisa ntshovelo na vuxopaxopi bya khwaliti hi xikongomelo xa ku endla minxaviso ya ximila. Xikongomelonkulu xa ku vona ku faneleka ku kula ka C. metuliferus exikarhi ka ti-greenhouse, nete ya ndzhuti na mimbangu ya le rivaleni ku endlela ku pfuneta nxopaxopo. Timbewu ta C. metuliferus ta nonon'hwa ku tihlukisa ehansi ka swiyimo swa mbangu leswi faneleke laha varimi va tirhaku eka tona. Ku hlukisa swi kamberiwe hi ku langutana na midiyamu ya ku kula, skarifikhexini na switifiketi swa timbewu. Swilo leswi swi lawula ntshovelo na khwaliti ya muhandzu. Xikongomelonkulu xa ndzavisiso lowu a ku ri ku lavisa hi vuyelo bya ku nyikiwa ka switifiketi, midiyamu ya ku kula (sand + vermiculite, peat TS1 and seedling mix) na skarifikhexini eka ku humelela ku hlukisa timbewu ta C. metuliferus E. Mey. ex naudin. Timbewu ti klasifayiwile ehansi ka tikhathegori ta mune to hambana, ku nga, treated certified, non-treated certified, treated uncertified, na non-treated uncertified.Hi vunharhu ka timediya leti ti ve na nhlukiso wa xiyenge hi 80%. Vuyelo byi kombise leswo treated certified na non-treated certified ti ve na ku humelela ka le henhla ka nhlukiso hi 93.6% na 91.3% hi ku landzelelana. Vuhumeleri bya nhlukiso wa le hansi ku ve timbewu ta treated uncertified hi vuyelo bya 37%. Vulehi bya 12 cm byi voniwe eka certified seedlings tanihi bya le henhla swinene. Swimilani swa unscarified na swa uncertified swi ve na timbewu ta le hansi, ta vulehi bya 3.44 cm eka vhiki ra vumune. Hikokwalaho, seed certification swi ve na vuyelo ngopfu ku tlula scarification hi majini ya le henhla swinene. Ku khomaniseka ka ximila eka bolo ya misava i swa nkoka eka ku pona no ya emahlweni ka ximila loko xi transplantiwa, kasi ndzavisiso lowu wu tlakusa leswo ku va na peat na timbewu leti nga na switifiketi tanihi ndlela yo antswa swinene ya ku kurisa swimila na ku va na swimila swa khwaliti. Ku pfumaleka ka mati, nkulo wa swilo hinkwaswo na ku cinca ka tlayimete i swa nkoka leswi khumbaka ku tirheka ka vurimi eka malembexidzana ya 20. Cucumis metuliferus yi kula hi ntumbuluko enhoveni; kambe ntshovelo wa yona wu angula eka ku kala ka mati, tindhawu to hambana ta ku rimiwa na mixaka ya misava, a swi si kamberiwa. Ku endliwe ndzavisiso ku vona ku faneleka ka mafambiselo ya ku kurisa ximila eka greenhouse, nete ya ndzhuti eka swiyimo swa mavala lama pfulekeke, leswo nxopaxopo wu ta kotlanisiwa eka tilevhele ta vuyelo bya ntshovelo loku nga endliwaka. Ndzavisiso wu endliwe eka greenhouse, nete ya ndzhuti na swiyimo swa mavala lama pfulekeke eKhempasi ya Sayense eUniversity of South Africa (Unisa) eFlorida, eGauteng (26.157831 S, 27.903364 E) hi nkarhi wa 2017/2018 na 2018/2019 hi tisizini ta ku byala. Ekspirimente leyi nga na swilo swimbirhi – ku nga misava ya loamy na misava ya misava ya sava ya loam) na levhele ya ncheleto wa mati (laha ku nga ri ku na mati kahle, laha ku nga na matinyana na laha ku kalaka mati). Xipirimente xa le mapotweni xi endliwe hi ndlela yo ka yi nga kunguhatiwangi hi ku tirhisa ku phindaphinda ka nkaye (9), na dizayini ya kona leyi nga kombisiwa laha henhla. Tipharamita ta ku pimiwa ti katsa chlorophyll content, stomatal conductance na xiyenge xa ntshovelo, xo fana na ku tirhisa mati, vuheleri bya biomass, biomass ehenhla ka bayomasi ya misava, indeksi ya ntshovelo, vulehi bya muhandzu, nhlayo ya mihandzu, na ku tirhisiwa ka mati hi ndlela yo hlayisa. Vuyelo byi kombise leswo tirhelo ra mavala lama pfulekeke swi pfanganisiwa na ndhawu yo kala mati na misava ya sava ya loam, swi ngetela nhlayo ya mihandzu. Ku tirhiwa ka swiyimo swa mavala lama nga pfuleka, swi hlanganisiwa na ndhawu yo kalanyana mati na misava ya sava ya loam, swi kombise ku tirhisiwa kahle ka mati ka le henhla hi (6. 2 kg m-3) loko swi kotlanisiwa na ku tirhiwa ku n'wana. I swa nkoka ku lemuka leswaku a ku va ngi na ku hambana ku kulu exikarhi ka misava ya sava ya loam na misava ya loam eka ntirhiso wa mati lowu ku nga water use efficiency (WUE). Kambe, misava ya sava ya loam yi kombise xiyenge xa le henhla xa WUE loko swi kotlangisiwa na misava ya loam. Hikokwalaho ku nga fikeleriwa eka mhaka ya leswo ku pfanganisa ku tirhana na mavala yo pfuleka, tilevhele ta ncheleto wa mati (kahle na le xikarhi) na misava ya sava ya loam swa bumabumeriwa eka varimi leswo ku ta fikelekeleriwa xiyenge xa le henhla xa WUE na ku humelela ka ntshovelo wa C. metuliferus. Ku hlengeletana ka tinutriyente eka ndhawu yin'we (nutritional concentration) ka swimila swi titshege hi swilo swo fana na leswi kumekaka eka mati, mbangu wa ku kula, masana ya dyambu na mixaka ya misava. Kambe, swilo swo fana na ku hlengeletana ka tinutriyente ta mihandu ya C. metuliferus a swi si tiveka. Xikongomelo xa ndzhavisiso a ku ri ku vona vuyelo bya tilevhele to hambana ta ku kala ka mati (ku pfumaleka ka mati, ku pfumalekanyana, na ku pfumaleka swinene ka mati), mixaka ya misava (misava ya loam na misava ya sava) mbangu wa ku kula (greenhouse, nete ya ndzhuti na mavala yo pfuleka) hi ku pfangana na tinutriyente eka mihandzu ya C. metuliferus E. Mey. ex naudin. Tisampuli ta mihandzu leyi nga omisiwa yi friziwa ti tirhisiwe eka ku endla vunyingi bya ꞵ-carotene, Vhitamini C, Vhitamini E, na total soluble sugars, ti-crude protein na ti-total flavonoids, total phenols, na micro-nutrients (Cu, Fe, Mn na Zn). Vuyelo bya ndzavisiso byi kombise leswo swimila leswi nga kurisiwa eka nete ya ndzhuti, swi pfanganisiwa na levhele ya nkalo wa mati swinene na misava ya loam, swi ngetele ti-soluble sugars hi (15.8 ˚Brix) loko ku kotlanisiwa na ku tirhiwa kun'wana. Swimila leswi nga hansi ka mbangu wa nete ya ndzhuti, swi pfanganisiwa na nkayivelonyana wa mati hi vuxikarhi na misava ya loam, swi ve na vuyelo bya ku ngetela crude protein content hi (6.31˚Brix). Ku tirhiwa ka nkayivelo wa mati swinene swi pfanganisiwa na misava ya loam ehansi ka swiyimo swa greenhouse swi ngetelele ꞵ-carotene content (1.65 mg/100 g-1 DW) loko swi kotlanisiwa na ku tirhiwa kun'wana. Ku tirhiwa ka ku kayivela ka mati swi pfanganisiwa na misava ya loam ehansi ka mbangu wa nete ya ndzhuti swi kombise ku ngeteleleka ka vhitamini C hi (33.1 mg 100 g-1 DW). Ku tirhiwa ka nkayivelo wa mati swinene swi pfanganisiwa na misava ya loam ehansi ka swiyimo swa mbangu wa greenhouse swi ngetelele vhitamin E hi (35.1 mg 100 g-1 DW) loko swi kotlanisiwa na ku tirhiwa kun'wana. Ku tirhiwa ka mavala lama nga rivaleni eha CE g-1 DW) loko swi kotlanisiwa na ku tirhiwa kun'wana. Ku tirhana na nkayivela mati ka levhela ya le xikarhi na misava ya sava ya loam ehansi ka mbangu wa nete ya ndzhuti swi kombise ku ngeteleleka ka Zn content (12.7 μg g-1 DW) loko swi kotlanisiwa na ku tirhiwa kun'wana. Vuyelo byi kombisa leswaku ximila lexi xi na mihandzu ya khwaliti yo antswa hi ku landza ku hlengeletana ka tinutriyeente na tikhonstituwenti ta bayokhemikali, loko xi kurisiwa ehansi na ku ka ku nga ri na nkayivela mati kumbe ku kayivelanyana ka mati, hi ku tirhisa misava ya loam kumbe misava ya sava eka nete ya ndzhuti na le ka mavala ya le rivaleni.Ti-primary betabolites ti tlhela titiviwa tanihi biological compounds leti ti faneleke eka ku kula na ku hluvuka ka ximila hi nkarhi wa vutomi bya xona. Ti na vuyelo byo kongoma eka ntshovelo na tikhonsticuwenti ta bayokhemikala eka swimila. Vunyingi bya primary metabolites swi vekiwe hi ku tirhisa LC-MS-8040 triple quadrupole mass spectrometer (Shimadzu) eka mihandzu leyi nga ntshovelo wa ku tirhiwa kun'wana loku ku nga vuriwa laha henhla. Vuyelo byi kombe leswo ku tirhana na nkala nkayivelo wa mati, swi pfanganisiwa na misava ya loam ehansi ka mbangu wa nete ya ndzhuti, swi ngetelele swinene asparagine content from 10×106 to 80x106 nsi ka nkayivelo swinene wa mati na misava ya loam, swi ngetelele ti-total mz loko swi kotlangisiwa na ku tirhiwa kun'wana. Ku tirhana na nkayivelo wa mati swinene, swi pfanganisiwa na misava ya sava ya loam ehansi ka mbangu wa mavala lama pfulekeke hi nkarhi wa sizini ya 2017/2018, swi ngetelele swinene dopa content ku suka eka 12,030 to 324,240 peak intensity, kasi hi nkarhi wa sizini ya 2018/2019 season, 4-hydroxyproline ku 10×106 to 90x106 peak intensity swi ngeteleleke swinene. Ku tirhana ko fanana ehansi ka mbangu wa greenhouse, swi ngetelele swinene acetylcarnitine content ku suka eka 3,761 to 82,841 area under the curve hi nkarhi wa sizini ya 2018/2019. Ku tirhiwa ka ku nga ri na ku kayivela ka mati ka le xikarhi swi pfanganisiwa na misava ya loam ehansi ka mbangu wa mavala lama nga rivaleni swi ngetelele swinene norepinephrine content from 71,577 to 256,1045 peak intensity. Ndzavisiso wu pimanyete leswo mpfanganyiso wa ku tirhana na tilevhele ta ncheleteo wa mati (laha ku nga ri ku na ku kayivela ka mati na le ku nga na nkayivelo wa mati) na misava ya loam na misava ya sava ya loam) ehansi ka greenhouse na nete ya ndzhuti swi khumbe swinene ku xifta ka mihandzu ya primary metabolites profile of C. metuliferus E. Mey. ex naudin loko ku langutaniwa na tifekthara ha yin'we yin'we hi ku landzelelana.flavonoids content (0.85 mg CE g-1 DW) loko swi kotlanisiwa na ku tirhiwa kun'wana. Ku tirhana na nkayivela mati ka levhela ya le xikarhi na misava ya sava ya loam ehansi ka mbangu wa nete ya ndzhuti swi kombise ku ngeteleleka ka Zn content (12.7 μg g-1 DW) loko swi kotlanisiwa na ku tirhiwa kun'wana. Vuyelo byi kombisa leswaku ximila lexi xi na mihandzu ya khwaliti yo antswa hi ku landza ku hlengeletana ka tinutriyeente na tikhonstituwenti ta bayokhemikali, loko xi kurisiwa ehansi na ku ka ku nga ri na nkayivela mati kumbe ku kayivelanyana ka mati, hi ku tirhisa misava ya loam kumbe misava ya sava eka nete ya ndzhuti na le ka mavala ya le rivaleni.Ti-primary betabolites ti tlhela titiviwa tanihi biological compounds leti ti faneleke eka ku kula na ku hluvuka ka ximila hi nkarhi wa vutomi bya xona. Ti na vuyelo byo kongoma eka ntshovelo na tikhonsticuwenti ta bayokhemikala eka swimila. Vunyingi bya primary metabolites swi vekiwe hi ku tirhisa LC-MS-8040 triple quadrupole mass spectrometer (Shimadzu) eka mihandzu leyi nga ntshovelo wa ku tirhiwa kun'wana loku ku nga vuriwa laha henhla. Vuyelo byi kombe leswo ku tirhana na nkala nkayivelo wa mati, swi pfanganisiwa na misava ya loam ehansi ka mbangu wa nete ya ndzhuti, swi ngetelele swinene asparagine content from 10×106 to 80x106 mz loko swi kotlangisiwa na ku tirhiwa kun'wana. Ku tirhana na nkayivelo wa mati swinene, swi pfanganisiwa na misava ya sava ya loam ehansi ka mbangu wa mavala lama pfulekeke hi nkarhi wa sizini ya 2017/2018, swi ngetelele swinene dopa content ku suka eka 12,030 to 324,240 peak intensity, kasi hi nkarhi wa sizini ya 2018/2019 season, 4-hydroxyproline ku 10×106 to 90x106 peak intensity swi ngeteleleke swinene. Ku tirhana ko fanana ehansi ka mbangu wa greenhouse, swi ngetelele swinene acetylcarnitine content ku suka eka 3,761 to 82,841 area under the curve hi nkarhi wa sizini ya 2018/2019. Ku tirhiwa ka ku nga ri na ku kayivela ka mati ka le xikarhi swi pfanganisiwa na misava ya loam ehansi ka mbangu wa mavala lama nga rivaleni swi ngetelele swinene norepinephrine content from 71,577 to 256,1045 peak intensity. Ndzavisiso wu pimanyete leswo mpfanganyiso wa ku tirhana na tilevhele ta ncheleteo wa mati (laha ku nga ri ku na ku kayivela ka mati na le ku nga na nkayivelo wa mati) na misava ya loam na misava ya sava ya loam) ehansi ka greenhouse na nete ya ndzhuti swi khumbe swinene ku xifta ka mihandzu ya primary metabolites profile of C. metuliferus E. Mey. ex naudin loko ku langutaniwa na tifekthara ha yin'we yin'we hi ku landzelelana. / Thuto ye e dirilwe ka maikemišetšo a go nyakišiša tiragatšo le boleng bja Cucumis metuliferus E. Mey. Ex Naudin (phara ya seAfrika) mo tikologong yeo e šireleditšwego le ya mo lebaleng e le nepo ya go bapetša tshekatsheko ya kotollu le boleng go hola thekišo ya mabele. Maikemišetšo kakaretšo e le go humana tsela ya maleba ya go mediša C. Metuliferus dipakeng tša mokhukhutšhireletšo, nnete ya moriti le mo ditikologong tša mabala ao a bulegilego gore go nolofatšwe tshekatsheko. Go boima go mediša dipeu tša C. Metuliferus ka tlase ga maemo a tikologo ya maleba ya go tlwaelega yeo e šomišwago ke bontši bja balemi. Medišo ya dipeu e lekanyeditšwe go ya le ka sedirišwa sa go mediša dimela, go fala dipeu le go hlahlobo ya boleng bja dipeu. Dikokwana tše ke tšona di laolago kotollu le boleng bja dienywa. Nepokgolo ya thuto ye e be e le go nyakišiša khuetšo ya tlhahlobo ya polokego ya dipeu tše, sedirišwa sa go mediša dimela (mohlaba+vermiculite, peat TS1 le motswako wa dipeu) le phalo ya dipeu go kgonthišiša katlego ya go mela ga dipeu tša C. Metuliferus. Dipeu di ile tša arolwa go ya le ka magoro a mane, bjalo ka peu ya go okobatšwa ka dikhemikhale yeo e hlahlobilwego, peu yeo e sa okobatšwago gomme e hlahlobilwe, peu ya go okobatšwa e sa hlahlobjwago le peu yeo e sa okobatšwago gomme e se ya hlahlobjwa. Boraro bja didirišwa tše di laeditše katlego ya go mediša yeo e ka balelwago go 80%. Dipoelo di šupa gore dipeu tšeo di okobaditšwego di se a hlahlobjwa le tšeo di sa okobatšwago di hlahlobilwe di bile le katlego ya tlhogo yeo e ka balelwago go 93.6% le 91.3%. Tlhogo ya fase e bile go dipeu tšeo di okobaditšwego di sa hlahlobjwago ka poelo ya 37%. Dipeu tše di hlahlobilwego di laeditše botelele bja 12cm gomme e le bjona bja go di feta ka moka. Dipeu tšeo di sa falwago le go hlahlobjwa di bile le botelele bja fase bja go balelwa go 3.44 cm ka dibeke tše nne. Bjalo, tlhahlobo ya dipeu e tlišitše katlego go fetiša phalo. Ka ge mudu wa dipeu o le bohlokwa go tšhutišetšo ya maphelo a dimela, thuto ye e thekga mmutedi le tlhahlobo ya dipeu bjalo ka tlhakanyo ya go mediša dimela tša boleng bja maleba. Tlhokego ya meetse, go oketšega ga baagi, le diphetogo tša klaemete ke tšona dikokwana tše di amago tšwelelo go tša temo nakong ya bjale. C. Metuliferus E. Mey. ex naudin e mela ka lešokeng tlhagong ya yona; efela, kotullo ya yona go tlhokego ya meetse, go mehuta ya mašemo le mehuta ya mabu ga se e ahlaahlwe. Thuto e ile ya dirwa go humana mokgwa wa go bjala/mediša dimela dipakeng tša mokhukhutšhireletšo, nnete ya moriti le boemo bja lebala le le bulegilego, gore go tle go tšweletšwe tshekatsheko yeo e laetšago diphapano tša mabato a puno. Nyakišišo ye e diritšwe ka fase ga maemo a mokhukhutšhireletšo, nnete ya moriti le lebaleng le le bulegilego Yunibesithing ya Afrika Borwa (UNISA) Khamphasing ya tša Saense, go la Florida, Gauteng (-26.157831 S, 27.903364 E) ka nako ya sehla sa 2017/2018 le 2018/2019 ka dinako tša go mela. Teko ye e ithekgile godimo ga dikokwana tše pedi – mabu (monola le mohlaba) le mabato a taolo ya go nošetša (tlhokego ya meetse ya lebato la fase, tlhokego ya meetse ye e lekanetšego le tlhokego ya meetse ya lebato la godimo). Diteko di be di beilwe ka mokgwa wo o sa rulaganywago gomme teko ye nngwe le ye nngwe e boeleditšwe ga senyane (9) bjalo ka ge e laeditšwe godimo. Dipharametha tšeo di lekantšwego di akaretša dikagare tša chlorophyll, stomatal conductance le bjalo ka tšhomišo ya meetse, palomoka ya dimela, dimela tše di bonagalago ka godimo, lenaneo la puno, botelele bja enywa, palo ya enywa le tšhomišo ya meetse ke dimela. Dipoelo di tšweletša gore teko ya mo lebaleng le le bulegilego le meetse a a lekanetšego gammogo le monola di oketša palo ya dienywa. Teko ya mo lebaleng le le bulegilego go kopantšhwa le meetse ao a lekanetšego le monola, di laeditše tšhomišo ya meetse yeo e balelwago go (6.2 kg m-3) ge go bapetšwa le diteko tše di ngwe. Go bohlokwa go lemoga gore ga ga go na diphapano magareng ga mohlaba le monola tšhomišong ya meetse (WUE). Efela, mohlaba o laeditše (WUE) ya godimo ge go bapetšwa le monola. Se se bolela gore, go ka tšewa sephetho sa gore teko ya dipeu lebaleng le le bulegilego, taolo ya go nošetša dimela (ye gabotse le ye e lekanetšego) le mohlaba ke didirišwa tšeo go eletšwago balemi gore ba di šomiše go humana (WUE) ya godimo le tšweletšo ye e atlegilego ya C. metuliferus. Bontši bja phepo mo mabeleng bo hlohleletšwa ke dikokwana tša go swana le meetse, tikologo ya mo a melago gona, dihlase tša letšatši le mehuta ya mabu. Efela, dikokwana tše di huetšago bontši bja diphepo go dienywa tša C. metuliferus ga dišo di tsebjwa. Nepo ya thuto ye e be e le go nyakolla khuetšo yeo dikokwana tše di latelago; di nago le yona go bontši bja diphepo go enywa ya C. Metuliferus: mabato a meetse (tlhokego ya meetse ya lebato la fase, tlhokego ya meetse ye e lekanetšego le tlhokego ya godimo ya meetse), mehuta ya mabu (monola le mohlaba) le tikologo ya go mediša (mokhukhutšhireletšo, nnete ya moriti le lebala le le bulegilego). Diteko tša enywa yeo e omišitšwego ka setšidifatšing e ile ya šomišwa go tšweletša boleng bja ꞵ-carotene, vitamin C, vitamin E, total soluble sugars, crude proteins, total flavonoids, total phenols, le micro-nutrients (Cu, Fe, Mn le Zn). Dipoelo di šupa gore dimela tše di godišitšwego ka fase ga nnete ya moriti, go akaretša le tlhokego ya meetse ya godimo le monola di nyološitše diswikiri tše di humanegago mo dimeleng (15.8 ˚Brix) ge go bapetšwa le diteko tše dingwe. Dimela tikologong ya nnete ya moriti go akaretša le tlhokego ya meetse ye e lekanetšego le monola di ile tša nyološa phroteine (6.31 ˚Brix). Teko go tlhokego ya meetse ya godimo go akaretša le monola ka tlase ga boemo bja mokhukhutšhireletšo go nyološitše diteng tša ꞵ-carotene (1.65 mg 100 g-1 DW) ge e bapetšwa le diteko tše dingwe. Teko go tlhokego ya meetse go akaretša monola ka fase ga nnete ya moriti go nyološitše Vitamin C (33.1 mg100 g-1 DW). Teko go hlokego ya meetse ya godimo go akaretša mohlaba tikologong ya mokhukhutšhireletši go nyološitše diteng tša vitamin E (35.1 mg/100 g-1 DW) ge e bapetšwa le diteko tše dingwe. Teko ya go se hlokege ga meetse, go akaretša le monola tikologong ya lebala le le bulegilego e nyološitše palomoka ya diteng tša phenolic (6.4 mg GAE/g-1 DW) ge e bapetšwa le diteko tše dingwe. Teko lebala le le bulegilego ka fase ga hlokego ya meetse ye godimo go akaretša monola go okeditše diteng tša flavonoids (0.85 mg CE g-1 DW) mo dienyweng tša gona ge e bapetšwa le diteko tše dingwe. Teko go hlokego ya meetse ye e lekanetšego le mohlaba ka fase ga nnete ya moriti di laeditše go oketšega ga diteng tša Zn (12.7 μg g-1 DW) ge e bapetšwa le diteko tše dingwe. Dipoelo di laetša gore semela se se thunya boleng bjo bo kgodišago bja dienywa ge go lebeletšwe bontši bja diphepo le dikokwana tša dikhemikhale ge di medišwa mo go sa hlokegago meetse go yela go mo go hlokegago meetse ka go lekanela, go šomišitšwe monola goba mohlaba mo nneteng ya moriti le mo lebaleng le le bulegilego. Dimetabolite tša motheo di tsebjwa bjalo ka motswako wa tlhago wo o lego bohlokwa go kgolo le tlhabollo ya dimela maphelong a tšona. Di na le khuetšothwii go dikokwana tša puno le khemikhale ya hlago ya dimela. Bontši bja dimetabolites tša motheo di humanwe ka go šomiša LC-MS-8040 triple quadrupole mass spectrometer (Shimadzu) ya go tšwa dienyweng tšeo di bunnwego ditekong tše di šetšego di boletšwe. Dipoelo di laeditše gore teko ya hlokego ya meetse ya lebato la fase go akaretša le mohlaba tikologong ya nnete ya moriti; e nyološitše asparagine content go tloga go 10×106 go ya go 80x106 peak intensity ge e bapetšwa le diteko tše dingwe. Tlhokego ya meetse ya lebato la fase e akaretša le monola tikologong ya lebala le le bulegilego ka nako ya sehla sa 2017/2018, 4-hydroxyproline go tšwa go 10×106 go ya go 90x106 area under curve e ile ya nyušwa. Teko ya go swana le ye tikologong ya mokhukhutšhireletšo e ile ya oketša dikagare tša acetylcarnitine go tšwa go 3,761 go ya go 82, 841 peak intensity ka nako ya sehla sa 2018/2019. Teko go tlhokego ya meetse ye e lekanetšego go akaretšwa le monola tikologong ya lebala le le bulegilego e nyološitše dikagare tša norepinephrine go tloga go 71,577 go ya go 256,1045 peak intensity. Diteko di šupa gore ge go kopantšwe taolo ya mabato a go nušetša (tlhokego ya meetse ya lebato la fase le tlhokego ya meetse ya lebato la godimo) le (monola le mohlaba) ka fase ga boemo bja mokhukhutšhireletšo le nnete ya moriti go ile gwa ama katološo ya dimetabolites tša motheo tša enywa ya C. metuliferus ge di bapetšwa le kokwana ye nngwe le nngwe. / College of Agriculture and Environmental Sciences / Ph. D. (Agriculture)

The access of water globally is becoming more strained, why the focus on industrial water use is increasing. The present study examined how industries should approach water efficiency projects, what organizational constraints that should be addressed, and what effects water saving measures have on economic costs, environmental impact and influence from water related risks. The study has been conducted at Saab Group. Primary data for water supply amount and cost has been obtained from twelve sites for the year of 2018. Data from these sites has been used to estimate the water use for the other 43 sites included in this study. Interviews with employees across Saab´s organization and with external stakeholders have functioned as important sources of information, combined with investigations of internal company documents. To facilitate for companies to structurally address water efficiency projects, the concept of the Deming Cycle is developed in this study. The steps included are necessary to address major identified organizational constraints which are lack of communication, lack of incentives for employees, and lack of economic incentives. Furthermore, with water often having energy embedded into it, a new Water Management Hierarchy is developed to include the interrelated aspects of energy supply and energy recovery. The potential for pipe leakages and the challenge to detect these are also identified. If the time from leak occurrence to repair in 2018 was eliminated, the total water supply in Arboga could have been reduced with 10100 m3 which corresponds to 35% of total supply to the site, respectively 35900 m3 and 42% in Björkborn. In Tannefors, water saving measures are identified for a surface treatment process, a facility with testing equipment, and by utilization of groundwater. Not all water saving measures result in reduced annual operating costs, due to an increased energy demand. Furthermore, if neglecting the possibility of energy recovery when aiming for water use reduction, the results show that replacing a once-through cooling system using potable municipal water as a medium with a dry-cooling unit, can increase greenhouse gas emissions. In 2018, the simultaneously implementable water saving measures in Tannefors would have reduced the water supply with 40600 m3, which corresponds to 22% of the total supply to the site. The greenhouse gas emissions would simultaneously have been reduced with 0.4 tonnes CO2eq. If also addressing energy supply reduction and energy recovery, some measures achieves a reduction of over 35 tonnes CO2eq, which results in enhanced economic viability from reduced operating costs. This study suggest that organizational constraints have to be addressed to successfully implement identified water saving measures. To allow economic motivation for all water saving measures in Tannefors, a payback period of over 7 years has to be applied, which can be lowered if the measure also reduces energy demand or increases energy recovery. In order to avoid sub-optimization of water saving measures, the current Water Management Hierarchy has to include the aspects of energy supply and energy recovery. If the aim is to reduce a corporation’s water use, the largest sites with heavy industrial processes should be addressed first. However, the potential impact from water related risks at smaller sites should not be neglected, in order to ensure safe operations and avoid increased costs in the company´s supply chain. / Tillgången av vatten blir alltmer ansträngd globalt, varför fokus på industriell vattenanvändning ökar. Den här studien undersökte hur industrier bör förhålla sig till vatteneffektivitetsprojekt, vilka organisatoriska begränsningar som bör hanteras, och vilka effekter vattenbesparande åtgärder har på ekonomiska kostnader, miljöpåverkan och påverkan från vattenrelaterade risker. Studien har genomförts på Saab Group. Primärdata för vattentillförselmängd och kostnad har erhållits från tolv platser för år 2018. Data från dessa siter har används för att uppskatta vattenanvändningen för de övriga 43 siterna som ingår i denna studie. Intervjuer med anställda inom Saabs organisation och med externa intressenter har fungerat som viktiga informationskällor, i kombination med undersökningar av interna företagsdokument. För att underlätta för företag att strukturellt ta itu med vatteneffektivitetsprojekt, så utvecklas Demingcykel-konceptet i den här studien. De inkluderade stegen är nödvändiga för att hantera viktiga identifierade organisatoriska begränsningar som är brits på kommunikation, brist på incitament för anställda och brist på ekonomiska incitament. Vidare, då vatten ofta är en energibärare, utvecklas en ny vattenminskningshierarki för att inkludera de sammanhängande aspekterna av energitillförsel och energiåtervinning. Potentialen för rörläckage och utmaningen att upptäcka dessa identifieras också. Om tiden från läckage till reparation under 2018 eliminerades, kunde den totala vattentillförseln i Arboga ha minskat med 10100 m3 vilket motsvarar 35% av total vattentillförsel till siten, respektive 35900 m3 och 42% i Björkborn. I Tannefors identifieras vattenbesparingsåtgärder för en ytbehandlingsprocess, en anläggning med testutrustning, och genom utnyttjande av grundvatten. Alla vattenbesparande åtgärder resulterar inte i minskade årliga driftkostnader, på grund av ett ökat energibehov. Vidare, om möjligheten för energiåtervinning förbises när reducering av vattenanvändning är målet, visar resultaten att ersättningen av ett kylsystem som använder kommunalt dricksvatten utan recirkulering med en luftkyld enhet, att utsläppen av växthusgaser kan öka. Under 2018, så skulle de simultant implementerbara vattenbesparande åtgärderna i Tannefors ha minskat vattentillförseln med 40600 m3, vilket motsvarar 22% av den totala tillförseln till siten. Utsläppen av växthusgaser hade samtidigt minskats med 0.4 ton CO2eq. Om även energitillförsel och energiåtervinning tas i beaktande, uppnår vissa åtgärder en minskning på över 35 ton CO2eq, vilket resulterar i förbättrad ekonomisk lönsamhet från minskade driftkostnader. Denna studie föreslår att organisatoriska begränsningar måste hanteras för att framgångsrikt genomföra identifierade vattenbesparande åtgärder. För att möjliggöra ekonomisk motivering för alla vattenbesparande åtgärder i Tannefors, måste en återbetalningstid på över sju år tillämpas, vilken kan sänkas om åtgärden också minskar energibehovet eller ökar energiåtervinningen. För att undvika suboptimering av vattenbesparande åtgärder, måste den nuvarande vattenhierarkin inkludera aspekterna av energitillförsel och energiåtervinning. Om målet är att minska ett företags vattenanvändning, bör de största anläggningarna med tunga industriprocesser först adresseras. Dock bör den potentiella påverkan från vattenrelaterade risker på mindre siter inte försummas, för att säkerställa säker drift och undvika ökade kostnader i företagets värdekedja.

Stickstoffdüngung und Bodenbewirtschaftung sind die Hauptquellen für Treibhausgase aus Anbausystemen. Dennoch sind diese Maßnahmen in Ackerbaubetrieben unerlässlich. In Anbetracht der Notwendigkeit, rasch Maßnahmen gegen den Klimawandel zu ergreifen, ist es notwendig Minderungspotentiale und Kosten in diesen Betrieben zu ermitteln und vergleichen. Es wurden Fallstudien in den USA, in Brasilien und in Deutschland durchgeführt, wobei jeweils eine Kultur in jeder Region untersucht wurde. Wissenschaftliche Literatur und Fokusgruppen mit lokalen Experten wurden genutzt, um realistische Ergebnisse zu generieren, die den lokalen Kontext abbilden. Diese Arbeit zeigt, dass die Wirtschaftlichkeit der Minderungsstrategien von dem betrachteten Zeithorizont abhängt, der sich aus der Kohlenstoffdynamik im Boden ergibt. Kurzfristig bieten Strategien, die die Kohlenstoffbindung fördern, ein größeres Minderungspotenzial, was jedoch langfristig nicht zutrifft. Kurzfristig wurden in Brasilien und den USA die geringsten Minderungskosten durch die Optimierung der Stickstoffdüngung erreicht. Diese Kosten sind negativ, was bedeutet, dass die Anwendung dieser Strategien, nicht nur ihre Emissionen, sondern auch ihre Kosten senken würden. Weitere kosteneffiziente Strategien waren die Verringerung der Bodenbearbeitungsintensität und Zwischenfrüchte, die in allen Fällen mit vergleichbaren Minderungskosten durchführbar waren und die Kohlenstoffbindung fördern. Der Einsatz von Hemmstoffen schließlich, der in den USA und in Brasilien möglich war, hatte die höchsten Minderungskosten. Langfristig betrachtet stiegen die Minderungskosten von Strategien mit Kohlenstoffbindung an und waren ähnlich hoch oder höher als bei Strategien ohne Kohlenstoffbindung. Da in allen Fällen dieselbe Methodik angewandt wurde, sind die Ergebnisse vergleichbar. Darüber hinaus sind die Ergebnisse zwar spezifisch für den Kontext, in dem sie berechnet wurden, sie liefern jedoch Erkenntnisse für ähnliche Regionen. / Nitrogen fertilization and soil management are the main sources of greenhouse gases from cropping systems. Yet these operations are essential on arable farms. Considering the need to take quick action against climate change, it is necessary to understand which mitigation potentials and cost can be attained in these farms and how they compare. Case studies in the USA, Brazil and in Germany were conducted, assessing one crop in each region. Scientific literature and focus groups with local experts were used to generate realistic results which depict the local context. This thesis identified that the economics of the mitigation strategies depended on the time horizon considered, which results from the carbon dynamics in the soil. In the short term, strategies promoting carbon sequestration offer a larger mitigation potential, yet this is not valid in the long term. In the short term, the lowest mitigation costs were attained by optimizing the nitrogen rate, feasible in the USA and Brazil. These costs are indicated to be negative, implying that adopting the strategy would not only lower emissions, but also reduce their costs. The next most cost efficient strategies were the reduction of the tillage intensity and cover crops, which were feasible in all cases with comparable mitigation costs and promote carbon sequestration. Lastly, the adoption of inhibitors, feasible in the USA and in Brazil, had among the highest mitigation costs. Assuming the long term, the mitigation costs of strategies with carbon sequestration increased, becoming similar to or higher than strategies without carbon sequestration. By applying the same methodology in each case, the results are comparable. Moreover, while the findings are specific to the context in which they were calculated, they provide insights for similar regions.

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