Depuis plusieurs années il est devenu essentiel de comprendre le déterminisme sexuel des espèces à fort intérêt économique afin d’optimiser leur production au sein d’écloseries émergentes.L’objectif principal de cette thèse était de mettre en évidence les mécanismes impliqués dans la détermination et la différenciation sexuelle, et notamment du sexe femelle, chez l’huître perlière P. margaritifera, espèce hermaphrodite protandre et espèce clé de la perliculture, la seconde ressource économique pour la Polynésie française. Pour atteindre cet objectif, deux approches ont été menées : une approche transcriptomique visant à étudier les mécanismes moléculaires du déterminisme et de la différenciation sexuelle, et une approche expérimentale visant à comprendre le phénomène de la sexualisation par des forçages environnementaux et hormonaux en s’intéressant plus particulièrement au déterminisme et à la différenciation sexuelle femelle.Dans l’approche transcriptomique, le transcriptome de la gonade de P. margaritifera a été séquencé à partir de plusieurs échantillons gonadiques d’huîtres de sexe mâle et femelle à différents stades de développement. Après le séquençage Illumina et l'assemblage du transcriptome, 70 147 contigs ont été obtenus. L’analyse fonctionnelle de ces 70 147contigs, a permis d’identifier des gènes d’intérêt et ainsi de constituer un catalogue de 87 ARNm codant pour 67 protéines impliquées dans la détermination, la différenciation sexuelle et/ou la gamétogenèse. Ensuite une analyse stricte des données de quantification RNAseq a révélé 1 937 contigs exprimés de manière différentielle entre les catégories histologiques des gonades. À partir de l’analyse de leurs profils d’expression au sein de chaque échantillon, un nouveau modèle de la reproduction de P. margaritifera, basé sur une double approche analytique, eg. histo-moléculaire, a été proposé. Ce modèle révèle notamment que le déterminisme sexuel de P. margaritifera chez l’adulte se produirait durant une phase de régression de la gonade. Considérant ainsi les nouveaux stades définis par ce modèle, 9 gènes biomarqueurs de la voie sexuelle femelle ont pu être identifiés révélant un modèle prédictif de la voie sexuelle basé sur 3 rapports d’expressions de gènes impliquant 2 gènes inconnus pmarg-c43476 et pmarg-c54338 et 2 gènes connus pmarg-foxl2 et pmarg-fem1-like. Ce deuxième modèle suggère fortement l'implication de pmarg-foxl2 et pmarg-fem1-like dans le déterminisme du sexe de P. margaritifera. Dans l’approche expérimentale, deux expérimentations séparées ont été réalisées pour mettre en évidence l’effet i) de plusieurs combinaisons de température et de niveau trophique, et ii) de l’œstradiol-17β administré par injection directe dans la gonade ; sur le sexe, la gamétogenèse et l’expression des neuf gènes biomarqueurs de la voie sexuelle femelle identifiés précédemment. Les résultats ont montré que la condition combinant la température de 28°C et la concentration en algues de 40 000 cellules mL-1 était la plus favorable non seulement à la maturation des gonades mâles et femelles mais aussi au maintien du sexe femelle. Ce serait dans cette condition environnementale qu’il serait possible d’induire un changement de sexe de mâle vers femelle. Dans la seconde expérimentation, il a été clairement démontré que la reproduction de P. margaritifera pouvait être régulée par les hormones œstrogènes. Les résultats montrent un effet négatif de l’œstradiol sur le développement et la différenciation mâle. Enfin les résultats du modèle prédictif de la voie sexuelle de P. margaritifera, suggèrent une programmation génétique du sexe femelle qui toutefois resterait soumise aux conditions environnementales validant ainsi l’hypothèse d’un mode de détermination mixte du sexe chez P. margaritifera. / For several years it has become essential to understand sex determination of species with high economic interest to maximize their production in emerging hatcheries.The main objective of this thesis was to identify the mechanisms involved in sex determination and sex differentiation, and particularly in female sex, in the pearl oyster P. margaritifera, a protandrous hermaphrodite species and the key species of the pearl farming, the second economic resource for French Polynesia. To achieve this goal, two approaches were undertaken: a transcriptomic approach to investigate the molecular mechanisms of sex determinism and sex differentiation, and an experimental approach to understand the phenomenon of sexualization by environmental and hormonal forcing focusing especially on female sex determinism and female sex differentiation.In the transcriptomic approach, the gonad transcriptome of P. margaritifera was sequenced from several samples of male and female oyster gonads at different stages of development. After Illumina sequencing and assembly of the transcriptome, 70,147 contigs were obtained. Functional analysis of these 70,147 contigs identified genes of interest and allowed the constitution of a catalog of 87 mRNAs encoding 67 proteins involved in sex determination, sex differentiation and/or gametogenesis. Then a strict analysis of RNAseq quantification data revealed 1,937 contigs differentially expressed between the histological categories of gonad. From the analysis of their expression profiles in each sample, a new model of reproduction of P. margaritifera, based on dual analytical approach, i.e. histo-molecular, has been proposed. This model shows that sex determination of adult P. margaritifera pearl oysters occur during a regression phase of the gonad. And considering the new stages defined on this model, 9 biomarkers genes of the female sexual pathway have been identified revealing a 3-gene-pair expression ratio based model, which makes it possible to predict the sexual pathway in this hermaphrodite species. This predictive model involves two unknown genes pmarg-c43476 and pmarg-c54338 and 2 known genes pmarg-foxl2 and pmarg-fem1-like, and strongly suggests the involvement of pmarg-foxl2 and pmarg-fem1-like in sex determinism in P. margaritifera.In the experimental approach, two separated experiments were conducted to demonstrate the effect of i) various combinations of temperature and trophic level, and ii) 17β-estradiol administered by direct injection into the gonad; on sex, gametogenesis and expression of the nine biomarkers genes of the female sexual pathway previously identified. The results showed that the condition combining a temperature of 28 °C and a concentration of 40 000 cells of algae mL-1 was the most favorable not only for the maturation of the male and female gonads but also for the maintenance of the female sex. It would be in this environmental condition that it would be possible to induce a sex change from male to female. In the second experiment, it was clearly demonstrated that the reproduction of P. margaritifera could be regulated by estrogen hormones. The results show a negative effect of estradiol on male development and differentiation. Finally the results of the predictive model of the sexual pathway of P. margaritifera, suggest a genetic programming of the female sex, which however remain subject to environmental conditions, thus validating the hypothesis of a mixed sex determinism mode in P. margaritifera.

This study explores how contemporary Afrikaans churches represent gender in their visual culture. For these purposes, a Barthean semiotic analysis is done on visual material produced between 2007 and 2008 by three Afrikaans corporate churches in the Pretoria- Centurion area, namely the Dutch Reformed congregations Moreletapark and kerksondermure (“church without walls”), and Doxa Deo’s Brooklyn and East campuses – Afrikaans Apostolic Faith Mission congregations. The analysis seeks to demystify and denaturalise the material’s potentially mythical, ideological and hegemonic underpinnings. Operating from an interdisciplinary theoretical framework comprising aspects of Visual Culture Studies and Gender Studies, this study primarily provides a focused analysis of the representation of men and masculinity in the selected churches according to three themes, namely professional occupation and leadership; physical activity and adventurism; and fatherhood. This focus was adopted owing to the lack of available literature on men and masculinity in the church and Christianity, as opposed to the more ready availability of research on women and femininity. The representation of gender in Moreletapark, kerksondermure and Doxa Deo is conceptualised in broad terms through a comparison of the representation of masculinity with femininity as its foil. In this regard gender is analysed in the three churches according to notions of gendered ontology and matters of work, marriage and family. Exscripted, or non-represented, themes are also problematised. It is argued that the churches under investigation represent gender in dualistic, essentialist and often stereotypical terms. This particular depiction of gender attests to the churches‟ participation in the biological essentialising of gender, polarising men and women into strict binary dualisms, whilst also visually denying the existence of homosexuality and alternative sexualities. This tendency is problematic, not only because it fails to provide a realistic portrayal of men and women in the three churches, but also because it visually participates in conservative and fundamentalist gender discourses. / Dissertation (MA)--University of Pretoria, 2013. / Visual Arts / unrestricted

Meloidogyne incognita est le nématode causant le plus de dégâts en agriculture. Sa particularité est d'être un organisme à reproduction asexuée obligatoire. Une femelle engendre des clones a priori 100% identiques génétiquement. Pourtant, M. incognita est capable de faire preuve d'une grande plasticité phénotypique lui permettant de répondre à de nouveaux environnements. Un exemple est le déterminisme du sexe, un phénotype lié aux conditions environnementales et semblant impliquer des régulations chromatiniennes. Un autre exemple est la capacité à contourner les résistances des plantes (virulence), un caractère héréditaire mais non-Mendelien. Dans le cadre de cette thèse, j'ai cherché à tester l'implication des mécanismes épigénétiques dans la plasticité phénotypique en absence de sexe de M. incognita. A ces fins, j'ai évalué la conservation des mécanismes épigénétiques chez les nématodes à galles. Cette approche a permis de pointer que les mécanismes connus chez C. elegans sont conservés chez les nématodes parasites de plantes. Puis, une méthodologie de ChIP-seq a été mise en place afin de comparer les profils d'accumulation des marques d'histones chez M. incognita au cours de la réponse aux conditions environnementales. Cette stratégie a permis la mise en évidence 1- de patrons d'histones modifiées marquant le développement du parasite et 2- de régions génomiques comportant plus de 300 gènes dont des candidats facteurs d'avirulence déjà décrits dans la littérature spécifiquement perdue entre M. incognita (a)virulents. Ces travaux de thèse présentent un intérêt fondamental sur la compréhension de l'évolution d'un organisme en absence de reproduction sexuée. / Meloidogyne incognita is the most damaging plant-parasitic nematode in agriculture. M. incognita reproduces in an asexual way by obligatory parthenogenesis. Genetically identical individuals develop from females and form clonal populations. Although these clones share the same genetic heritage, modifications of their phenotype can be observed when they are exposed to unfavorable environments. This phenotypic plasticity is characterized through two phenotypes of interest: sex-differentiation and virulence (i.e. capacity to parasite a resistant crop). Sex-differentiation varies among environmental conditions and was reported to be linked to decondensed chromatin regions. Virulence is an heritable character transmitted in a non-Mendelian way. Our study focuses on identifying the role of epigenome in the generation of phenotypic variability. To this end we detailed the presence of proteins involved in epigenetic regulations in Meloidogyne spp. We also developed a ChIP-seq assay to compare histone modifications between different developmental stages and between virulent and avirulent parasites. Our results allow to detect specific histone patterns associated with M. incognita development. These results lead us to propose a model that could explain sex determination in M. incognita. We also could link virulence acquisition with the loss of some specific genomic regions that contains more than 300 genes including already described potential avirulence factors. This study opens the way for analyzing the role of epigenetic mechanisms at a whole genome scale, and allows to identify novel biological processes involved in phenotypic variation in asexual organisms.

My doctoral thesis examines the broad question of whether appropriately designed institutions and policies can address the short- and long-term consequences of determinants of human capital which are "immutable'' by nature or are perceived as such. I consider three different types of immutable determinants of human capital: male versus female biology; colonization; and traditional norms of gender roles. In Chapter 1, I examine whether, and how, change in political regime type affects excess male infant mortality. Analyzing data on more than 3 million live births from sub-Saharan African countries, I exploit within-mother variation in political regime type to find that excess male infant mortality significantly decreases following a transition to democracy. I identify competitiveness of executive recruitment, constraints on the chief executive, and political participation as the features of democracy that matter most. Examining causal mechanisms, I find that democracy fosters the provision of health inputs, including maternal education, tetanus immunization, breastfeeding, and normal birth weight, all of which have stronger health benefits for boys than for girls, despite being found to be ex-ante "gender-neutral'' in my setting. In Chapter 2, I examine how colonial reproductive laws interact with market incentives to shape long-term fertility behavior in Africa. Exploiting the arbitrary division of ancestral ethnic homelands and the resulting discontinuity in institutions across the British-French colonial borders, I find that women in former British areas are more likely to delay sexual debut and marriage, and have fewer children. However, these effects disappear in areas close to sea, where market access and the opportunity cost of childbearing appear to be high irrespective of the colonizer identity. This heterogeneous impact of colonial origins extends to measures of local economic development and household welfare. Examining causal mechanisms, I argue that the fertility effect of colonial origins is directly linked to colonial population policies and reproductive laws and their impact on the use of modern methods of birth control. I find little evidence that the fertility effect of British colonization operates through education or income. While British colonization is linked to higher female education levels, this occurs mainly close to the sea while the fertility effects do not. Again, while income levels differ, the fertility gap between British and French colonies opened prior to 1980, while the income gap opened-up after 1990. This chapter highlights the heterogeneous nature of the colonial origins of comparative fertility behavior and economic development, and implies that economic incentives may overcome historical determinism. In Chapter 3, I examine the interplay between legal origins and pre-colonial cultural norms of gender roles in determining female economic empowerment in sub-Saharan Africa. Taking advantage of the arbitrary division of ancestral ethnic homelands across countries with different legal origins, I directly compare women among the same ethnic group living in civil law countries and common law countries. I find that women in common law countries are significantly more educated, are more likely to work in the professional sector, and are less likely to marry at young age. However, these effects are either absent or significantly lower in settings where ancestral cultural norms do not promote women's rights and empowerment. In particular, I find little effect in bride price societies, patrilocal societies, and societies where women were not involved in agriculture in the past.

Vad innebär egentligen social hållbarhet? Forskningen presenterar en rad olika förklaringar av begreppet, menar att det bland annat är möjligt att se begreppet som ett ramverk eller verktyg, men det kan även ses som ett sätt att uppnå hållbarhet i generell mening - men är dessa sätt att betrakta social hållbarhet något som även används i kommunernas praktiska verksamhet? Syftet med denna uppsats har varit att undersöka hur begreppet social hållbarhet används och beskrivs på kommunal nivå. Undersökningen har utgått från ett diskursteoretiskt perspektiv och en diskursanalytisk metod som syftar till att dekonstruera och analysera användningen av social hållbarhet på kommunal nivå. Inom ramen för denna uppsats är det två kommuner som har undersökts; Haninge och Huddinge kommun. Undersökningen och analysen av de båda kommunerna har visat att det finns många likheter i hur de båda kommunerna väljer att beskriva social hållbarhet. Analysen visar att det hos kommunerna finns en tilltro till att den fysiska planeringen ska kunna hjälpa kommunerna att skapa social hållbarhet, detta mestadels på liknande sätt. Beskrivningarna för hur social hållbarhet ska skapas i de båda kommunerna är likartade och visar tendenser på att vara ”stigberoende”.

The diploma thesis The Images of a City: the Resident's View of the Town of Nymburk deals with the issue of why people behave differently. The thesis is inspired by the approach of behavioral geography, which is represented by Kevin Lynch's work. Lynch has proposed the concept of the image of the city, the people's vision about the surroundings of a place where they live. The image of the city consists of its visible features evoking a strong impression and having an effect on people's sensory perception. Moreover, the direct influence of the city represented by architectural-urbanistic determinism and environmental psychology is also taken into account. I have found out during the research that both of theoretical approaches are important for identifying the relation between the town and his residents which relates to the behavior in the town. The aim of the diploma thesis is to find out which images of the town of Nymburk its inhabitants have, which components create their images and what is the composition of these components. The thesis focuses both on universal image of Nymburk and on images of the town with regard to the current lifecycle stage and respondents' sex. The research is made by the qualitative method using the mental maps and the semi-structured interview with sixteen respondents...

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Master's thesis is dealing with translation based on coupled finite automaton model. Coupled finite automaton contains input and output automaton. The input automaton makes syntactic analysis with an input string. Used rules from the input automaton control the output automaton, which generates an output string. In thesis is described a way of determinisation of the input automaton without loss of information about rules used in original automaton. The determinizitaion is divided into two parts - for finite and infinite translation specified by transducers. Then is presented a new pair automaton with increased computing power. This increased computing power consists in replace of input or output or just a part of automaton by context free grammar.

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Convencido que el desconocimiento de la historia y de nuestro entorno, nos conduce a resolver por caminos que restan y no aportan; olvidando que la naturaleza provee; más no castiga. El siguiente proyecto de tesis; ``Museo de los Fenómenos Naturales – Malecón Rivera Monserrate``, es una investigación arquitectónica que pretende unir las tipologías de museo y malecón. El proyecto busca ser un ejemplo de la prevención del ``desastre``, tanto por las exposiciones que no pretenden una falsa ecología, zonas de investigación y por sobretodo la volumetría. Esta última, contempla relacionarse conteniendo las crecidas del río Rímac, sin imponerse ni al entorno natural ni al contexto urbanístico histórico de la ciudad de Lima. Para ello utilizará elementos constructivos, como la tajamar, techos caminables y equipamientos como huertos inundables. La idea de utilizar elementos y conceptos constructivos, hoy olvidados; se basa en el uso de tres énfasis; Determinismo ambiental, Edificaciones calendario y Ciclos de retroalimentación. El solar para desarrollar el proyecto, está ubicado en la zona abandonada de la estación Monserrate, Cercado de Lima. La selección del terreno se fortalece con dos planes Municipales; La iniciativa de recuperación de la plaza Monserrate y la restauración de viviendas patrimoniales Lima 2035. El proyecto pretende ser una opción viable tras el desarrollo de Lima 2035. / Convinced that the ignorance of history and our environment, leads us to resolve by paths that remain and do not contribute; forgetting that nature provides; more does not punish. The following thesis project; `` Museum of Natural Phenomena - Malecón Rivera Monserrate '', is an architectural investigation that aims to unite the typologies of museum and boardwalk. The project seeks to be an example of the prevention of the `` disaster '', both for the exhibitions that do not pretend a false ecology, research areas and above all the volumetry. The latter, contemplates relating by containing the floods of the Rímac river, without imposing either the natural environment or the historical urban context of the city of Lima. For this, it will use construction elements, such as the cutwater, walkable roofs and equipment such as flooded orchards. The idea of using elements and constructive concepts, today forgotten; it is based on the use of three emphases; Environmental determinism, Calendar buildings and Feedback loops. The site to develop the project is located in the abandoned area of the Monserrate station, Cercado de Lima. The selection of the land is strengthened with two Municipal plans; The initiative for the recovery of Plaza Monserrate and the restoration of Lima 2035 heritage homes. The project aims to be a viable option after the development of Lima 2035. / Tesis