More and more schools worldwide provide their students with an individual digital tool, investments called 1:1 programs. However, there is still limited knowledge about the causal effects of such programs on students’ education outcomes, especially for high-income countries. This paper examines how the implementation of 1:1 programs affects pupils’ average academic performance in primary school (grade 3) in Sweden between 2014/2015-2018/2019. The data is collected from the Swedish National Agency for Education and the Institute for evaluation of labour market and education policy (IFAU) on school level. I use a difference-in-difference design to estimate the effect of 1:1 programs on pupils’ academic performances. The main results in this study indicate that 1:1 initiatives have a small positive effect on student's academic performances in Swedish and mathematics on average, although the effect varies depending on the subtests investigated.

The dissertation is focused on the issue of implementation and evaluation of the laboratory tests fiber reinforced concrete. The issue of steel fiber is solved in terms of their physico-mechanical parameters, which are an important part of their design and reliable operation in the product design. The experimental part of the thesis is focused on laboratory tests of steel fiber of different strength classes and different weight benefits of steel fibers, the default reference mixture of plain concrete are certified mixtures commonly used as structural concrete in construction. The evaluation of the testing of samples produced steel fiber is made by three selected recommending regulations for the design and testing of structural steel fiber. In conclusion, the individual obtained experimental results are compared.

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The challenges of at-risk students are not new. Newspaper articles from the 1860s presented information about communities seeking to help students to complete school and find employment to provide a livable wage. Today's solutions focus on legislation intended to affect societal change and provide equitable opportunities for at-risk students. Much research regarding how to improve academic outcomes for at-risk students addresses high school level, identifying those factors that encourage secondary learners to remain in school. However, less work has been done investigating whether earlier intervention can obviate later retention efforts by improving students' learning outcomes in the elementary grades. In this vein, engagement is a factor found to positively influence learning, particularly when students are actively engaged with instructional content. Technology can facilitate such interactions between students and content; however, research is needed to better understand the relationship between student engagement and technology, particularly with at-risk students in elementary settings. Seeking to address the gap, this qualitative study examined the occasion of a fifth-grade school that recently implemented 1:1 technology. Using a case study approach, researchers explored the effects of the 1:1 Chromebook implementation on teacher-perceived student engagement at the elementary level. This study sought to better understand how this school technology application influenced student engagement including constructs such as relevance, novelty, and gamification. Teachers in the study expressed that their students' engagement levels increased with Chromebook use. They identified relevance, autonomy, and novelty as reasons for students' engagement with the technology.

A variação do número de cópias (CNV) de genes e cromossomos é uma característica comum do genoma plástico de Leishmania major, que pode estar associada à resistência do parasita à quimioterapia das leishmanioses. Em outros eucariotos, alterações na replicação do DNA ou na resposta a danos no DNA (DDR) pode levar à CNV. Nestes organismos, o complexo de checkpoint 9-1-1 (RAD9, RAD1 e HUS1) é essencial para a detecção e a sinalização do estresse de replicação e para o recrutamento de uma apropriada DDR. Já demonstramos que L. major expressa um homólogo 9-1-1 funcional. Aqui, avaliamos a deficiência de subunidades de 9-1-1 na variação do número de cópias em células selecionadas em metotrexato (MTX), um inibidor da enzima diidrofolato redutase timidilato sintetase (DHFR-TS). A seleção em MTX facilita o isolamento de células que carregam amplificações contendo o locus da DHFR-TS. Assim, selecionamos células deficientes de HUS1 ou RAD9 para resistência ao MTX sem e com exposição previa a hidroxiureia (HU), uma droga que causa estresse de replicação por inibição da ribonucleotídeo redutase, e avaliamos o efeito da deficiência destas proteínas na CNV e no tipo de amplificação gerada. Avaliamos também o efeito da deficiência destas proteínas no processo de síntese do DNA medido pela incorporação de IdU e observamos que a deficiência destas proteínas levou a um incremento na síntese do DNA na ausência de estresse de replicação e a perfis opostos de síntese do DNA após a remoção do estresse replicativo. Análises da detecção de simples fita do DNA (ssDNA) e da histona H2A fosforilada (?H2A) como indicadores do processo de estresse de replicação e dano no DNA também foram conduzidas. Em conjunto, nossos resultados indicam que (i) os níveis alterados das proteínas HUS1 e RAD9 afetam o padrão da CNV após a seleção no MTX, assim como a natureza da amplificação; (ii) HUS1 e RAD9 parecem possuir mecanismos distintos para mediar a CNV; (iii) a função destas proteínas na CNV deve envolver o processo de replicação e (iv) HUS1 e RAD9 são requeridas para a manutenção da estabilidade genômica em Leishmania. Estes resultados contribuem para uma melhor compreensão não só da evolução da via de sinalização mediada pelo complexo de checkpoint 9-1-1 nos eucariotos, mas também da bases moleculares da plasticidade genômica e do fenômeno de amplificação gênica em Leishmania. / The copy number variation (CNV) of genes and chromosomes is a common feature of the plastic genome of Leishmania major, which is normally associated with resistance of the parasite to the chemotherapy of leishmaniasis. In other eukaryotes, alteration in DNA replication and DNA damage response (DDR) causes CNV. In these organisms, the RAD9-RAD1-HUS1 (9-1-1) checkpoint complex is essential for detection and signaling of replication stress and recruitment of an appropriate DDR. We have already demonstrated that L. major expresses a functional 9-1-1 homolog. Here we evaluated the effect of 9-1-1 subunit deficiency in CNV of cells selected in methotrexate (MTX), an inhibitor of the dihydrofolate reductase thymidylate synthetase (DHFR-TS) enzyme. Selection in MTX facilitates the isolation of cells that carry amplicons containing the DHFR-TS locus. Thus, we selected HUS1 or RAD9 deficient cells for MTX resistance without and prior exposure to hydroxyurea (HU), a drug that causes replication stress due to inhibition of ribonucleotide reductase, and evaluated not only CNV, but also the nature of the amplification generated. We also evaluated the effect of deficiency of these proteins in the DNA synthesis process measured by IdU incorporation and observed that the deficiency of these proteins led to an increase in DNA synthesis in the absence of replication stress, and to opposite profiles of DNA synthesis after removal of replicative stress. Analyzes of single-stranded DNA (ssDNA) and phosphorylated histone H2A (?H2A) as indicators of replication stress and DNA damage were also conducted in both presence and absence of replicative stress. Taken together, our results indicate that (i) altered levels of HUS1 and RAD9 proteins affect the CNV pattern after selection in MTX, as well as the nature of amplification; (ii) HUS1 and RAD9 possibly have different mechanisms to mediate CNV; (iii) the function of these proteins in CNV seems to involve replication process and (iv) HUS1 and RAD9 are required for the maintenance of genomic stability in Leishmania. These findings contribute to a better understanding not only of the evolution of the signaling pathway mediated by 9-1-1 checkpoint complex in eukaryotes, but also of the molecular basis of the genome plasticity and the gene amplification phenomenon in Leishmania.

Este trabalho tem como objetivo apresentar um estudo detalhado dos fundamentos da Geometria Complexa, ressaltando seus aspectos geométricos, topológicos e analíticos. Começando com materiais preliminares, como resultados básicos sobre funções holomorfas de uma ou mais variáveis e a definição e primeiros exemplos de variedades complexas, passamos a uma introdução à teoria de feixes e sua cohomologia, ferramenta indispensável para o restante do trabalho. Após um estudo sobre fibrados de linha e divisores damos atenção à Geometria de Kähler e alguns de seus resultados centrais, como por exemplo o Teorema da Decomposição de Hodge, o Teorema ``Difícil\'\' e o Teorema das $(1,1)$-classes de Lefschetz. Em seguida, nos dedicamos ao estudo dos fibrados vetoriais complexos e sua geometria, abordando os conceitos de conexões, curvatura e Classes de Chern. Terminamos o trabalho descrevendo alguns aspectos da topologia de variedades complexas, como o Teorema dos Hiperplanos de Lefschetz e algumas de suas consequências. / The main goal of this work is to present a detailed study of the foundations of Complex Geometry, highlighting its geometric, topological and analytical aspects. Beginning with a preliminary material, such as the basic results on holomorphic functions in one or more variables and the definition and first examples of a complex manifold, we move on to an introduction to sheaf theory and its cohomology, an essential tool to the rest of the work. After a discussion on divisors and line bundles we turn attention to Kähler Geometry and its central results, such as the Hodge Decomposition Theorem, the Hard Lefschetz Theorem and the Lefschetz Theorem on $(1,1)$-classes. After that, we study complex vector bundles and its geometry, focusing on the concepts of connections, curvature and Chern classes. Finally, we finish by describing some aspects of the topology of complex manifolds, such as the Lefschetz Hyperplane Theorem and some of its consequences.

A estabilidade genômica é condição essencial à sobrevivência e ao funcionamento dos organismos vivos. No entanto, várias situações podem provocar danos no DNA. Por exemplo, cerca de 104 lesões podem ocorrer no material genético de uma célula de mamífero a cada dia. No intuito de preservar a integridade genômica e contornar os efeitos deletérios destas modificações, uma maquinaria constituída de proteínas especializadas em reconhecer e reparar estes danos foi selecionada ao longo do curso evolutivo. Defeitos em proteínas destas maquinarias causam instabilidade genômica e pode resultar em elevada taxa de mutações e quebras do DNA que resultam em eventos de amplificação gênica, como em células cancerosas. De uma maneira aparentemente contrária ao requerimento de estabilidade genômica como condição primordial para a perpetuação da vida, Leishmania apresenta um genoma notavelmente maleável e explora a amplificação gênica como recurso de sobrevivência. Ainda que a plasticidade genômica em Leishmania seja facilmente demonstrada, nós não conhecemos os mecanismos precisos pelos quais este parasita coordena a ação da maquinaria de detecção de danos no DNA e a consumação dos eventos de amplificação gênica. No intuito de contribuir para a compreensão deste processo, nós identificamos proteínas homólogas do complexo 9-1-1 (Rad9-Hus1-Rad1) em Leishmania major. As proteínas LmHus1 e LmRad9 apresentam marcada divergência estrutural em relação aos seus homólogos em outros eucariotos e nenhuma proteína obviamente homóloga a Rad1 foi identificada neste parasita. Análises filogenéticas indicam que LmHus1 e LmRad9 são relacionadas ao complexos heterotriméricos envolvidos na detecção de danos no DNA. Em acordo com isso, nossos experimentos demonstram que alteração nos níveis destas proteínas interfere na capacidade do parasita em lidar com estresse genotóxico. LmHus1 localiza-se no núcleo, é requerida para o crescimento normal deste parasita e a diminuição de sua expressão compromete mecanismos de controle de ciclo celular e manutenção de telômeros. LmRad9 também localiza-se no núcleo e sua superexpressão causa defeito de crescimento e de resposta ao estresse genotóxico em L. major. Nós observamos que LmHus1 e LmRad9 formam um complexo responsivo ao dano no DNA in vivo, uma forte indicação de que o complexo 9-1-1 tenha sido conservado em L. major. As peculiaridades estruturais destas proteínas sugerem que o complexo 9-1-1 de L. major possua uma arquitetura distinta em comparação aos eucariotos superiores. Em adição a isto, outras proteínas, tais como a LmRpa1, também apresentam uma marcante divergência estrutural. Isso sugere que a via de sinalização de danos no DNA envolvendo o complexo 9-1-1 e Rpa1 de L. major possua mecanismos peculiares de ação. Estas observações podem permitir entender como ocorreu o processo evolutivo da sinalização mediada pelo complexo 9-1-1 nos eucariotos, além de ajudar para o entendimento das bases moleculares de como este parasito conduz os eventos de amplificação gênica. / Genome stability is a essential condition for survival and proper functioning of living organisms. However, a broad range of elements may lead to DNA damage. For instance, about 104 DNA lesions may be inflicted upon any given mammalian cell everyday. In order to maintain the genome integrity and circumvent the deleterious effects of these lesions, a molecular machinery composed of proteins specialized in detecting and repairing DNA damage has been selected in evolution. Defects of the proteins that constitute such machineries may result not only in a high mutation rate, but also in breaks in the DNA structure that can mediate gene amplification as observed in cancer cells. In an apparent opposition to such requirement for stability as an essential condition to life, the protozoan Leishmania presents a highly malleable genome and explores genome amplification as a survival and adaptation tool. Despite of the fact that the Leishmania genome plasticity can be easily demonstrated, the precise mechanisms that coordinate the molecular machineries involved in the detection and signaling of DNA damage, and in the regulation of gene amplification is still largely unknown. In order to contribute to a better understanding of these processes, we identified and studied the Leishmania major proteins that are homologues of those proteins that compose the 9-1-1 complex (Rad9-Hus1-Rad1). The proteins LmHus1 and LmRad9 present a high structural divergence when compared to its homologues from other eukaryotes and no obvious homologue of Rad1 was identified in the parasite genome. Phylogeny analysis indicated that LmHus1 and LmRad9 are closely related to heterotrimeric complexes involved in the detection of DNA damage. In accordance to that, our experiments demonstrated that altered levels of these proteins interfere with the parasite ability to deal with genotoxic stress. Moreover, LmHus1 was localized to the parasite nucleus and is a required protein for normal parasite proliferation. Besides, we showed that decreased levels of LmHus1 compromise cell cycle regulation and the maintenance of telomeres. LmRad9 was also shown to be localized to the cell nucleus and its overexpression led to growth defects and affected the L. major response to genotoxic stress. We also observed that LmHus1 and LmRad9 interact with each other to for a protein complex that is responsive to DNA damage in vivo, which strongly suggested that the 9-1-1 complex was conserved in L. major. The structural peculiarities of these proteins indicate that the possible L. major 9-1-1 complex has a different architecture when compared to the complex found in higher eukaryotes. In addition to that, other proteins, such as LmRpa1, also present a marked structural divergence. Altogether, these findings suggest that the DNA damage signaling pathway involving the 9-1-1 complex and LmRpa1 in L. major, may present a peculiar mode of action. These observations may contribute to a better understanding not only of the evolution of the signaling pathway mediated by the 9-1-1 complex in eukaryotes, but also of the molecular basis of the genome plasticity and the gene amplification phenomenon.

隨著行銷方式由大眾行銷演進至區隔行銷，現今已進入1-1行銷的時代。由於媒體廣告的過多，其實際的廣告效果已漸受質疑。行銷的手法不再是拍一支好的廣告片就可以擄獲所有消費者的購買，加上社會結構、型態的改變，科技的突飛猛進，消費者可選擇的產品種類愈來愈多，因此行銷競爭壓力更甚以往，而如何「有效」吸引消費者的購買便成為今日企業的重要課題。「資料庫行銷」的導入，可以透過有系統的分析，更精確地鎖定目標客群，進而從節省行銷成本與提升銷售業績雙方面，替公司帶來良好的利潤貢獻，也解決了企業銷售上的困境。 本研究屬於探索性研究，全文共分為六個章節。從資料庫行銷的相關議題方面探究，經過文獻探討、融入實務經驗、產生研究架構，並做為個案研究的訪談依據，以了解現行金融業在資料蒐集、資料庫軟硬體上的選用考量與管理、資料分析方法的運用程度、行銷計劃的執行及售後顧客管理上的態度與手法。整體架構在透過四家銀行：陽信商業銀行、中國信託商銀、玉山銀行及匯豐銀行的實證後，發現架構的適用性極高，充分兼顧了學理與實用性。另一面，環顧過去許多文獻中有關資料庫行銷的整體規劃模式，在構面上均多少有所不足。因此本研究乃融合文獻中之許多說法及實務運作所發展出，以補坊間書籍之缺。而在行銷作法上乃將傳統行銷學理的精神轉化為實際可執行的行動，分別從「潛在顧客開發」與「現有顧客滲透」兩方面依序開列步驟，更利從業人員落實資料庫行銷的執行。 經由第四章的研究分析可發現，目前國內在資料庫行銷的導入上尚未臻成熟，以金融業為例，中國信託商銀挾龐大信用卡顧客資料，加上公司本身的積極投入與耕耘，目前在這方面的發展腳步居領先優勢。因此資料庫行銷帶來的績效使金融同業莫不領會到導入此作法的必要性。以目前行銷手法觀之，外國銀行原先的資料庫行銷優勢將漸被本國銀行趕上，而本國銀行在售後顧客管理上又不如外國銀行佳。 理論架構的發展雖極具實用性，惟發展之過程仍不免有抽樣及深度等客觀因素上的限制。在後續研究上，建議未來的研究學人可自架構中個別議題上深入研究、或分析不同銀行對相同金融商品採資料庫行銷後的成果差異、或選個別單一公司做導入前後的研究，真正驗證資料庫行銷模式引進後的實際差異。

Turismaktiviteter till havs har kommit att få ett ökat intresse de senaste åren, där människor har upptäckt fördelen med kryssningsfärjor som transportmedel. De erbjuder ett varierat utbud av aktiviteter och upplevelser ombord, som både privat- och affärsresenärer kan ta del av. Detta omfattar flera kundgrupper med olika behov, vilket innebär att rederierna måste uppfylla kundernas förväntningar och vad de efterfrågar. I nuläget finns det en bred konkurrens på marknaden, där de olika aktörerna försöker skapa en egen nisch genom olika marknadsföringsstrategier i syfte att skapa långsiktiga relationer. Syftet med vår studie är att utreda hur Stockholms tre största rederier, Tallink Silja AB, Viking Line AB och Birka Cruises AB, arbetar för att behålla och vårda sina befintliga resenärer, samt vilka strategier som används för att nå nya kundsegment. Vi har även utgått ifrån kundens perspektiv för att undersöka hur de förhåller sig till företagens produkter och tjänster. Studien baseras på intervjuer med ledningsgruppen på respektive bolag, samt kundundersökningar. Relevanta teorier har valts ut för att analysera hur marknadsföringsstrategier i teorin stämmer överens med verkligheten. Trots att de undersökta kryssningsbolagen erbjuder likartade produkter och tjänster, visade resultatet att företagens affärsidé och varumärke skapade ett specifikt behov hos deras kunder. Detta medförde att företagets marknadsföringsstrategier var utmärkande på individnivå och därmed skapades ingen hög konkurrens mellan de berörda kryssningsbolagen. Samtliga rederier är under utveckling, där de fortfarande arbetar med att ständigt utveckla och anpassa sina kunderbjudanden för att bli ledande aktörer på marknaden. För att åstadkomma detta måste företaget upprätthålla sina imageskapande idéer genom att behålla bra service och erbjuda den kvalité som kunderna efterfrågar.

I de fall enskilda aktieägare tillfogas skada, genom organledamots handlande, innehar dessa rättigheten att påkalla skadeståndsansvar enligt 29:1 1 st. 2 men. ABL. I den svenska rättstillämpningen ter sig dock tillämpningen av paragrafen, i samband med indirekta skador, problematisk. Problematiken hänför sig till vilka regler, vars åsidosättande, aktualiserar tillämpningen av paragrafen tillsammans med aktieägares talerätt. Två tolkningar kan i fallet identifieras, vilka framförts inom doktrinen, varvid en begränsad och en mer liberal. Även om den liberala tolkningen är förenad med viss problematik, är denna enligt mitt förmenande den vilken bör tillämpas. En sådan tillämpning skulle således innebära att enskilda aktieägare tillerkänns talerätt vid indirekt skada, då regler vilka ger uttryck för normskyddsläran och bolagsledningens lojalitetsplikt åsidosatts. Rättsutvecklingen har vidare givet upphov till frågan, huruvida en ”indirekt” indirekt skada kan omfattas av paragrafens tillämpningsområde. En sådan skada uppstår särskilt i koncernförhållanden då dotterbolaget tillfogas en direkt skada, vilken därigenom åsamkar aktieägarna i dess moderbolag en ”indirekt” indirekt skada. Skadan faller utanför paragrafens tillämpningsområde, då de skadedrabbade inte utgör aktieägare i dotterbolaget. En lösning enligt mitt förmenande, varigenom paragrafens tillämpning koncernanpassas, är att principen om ansvarsgenombrott i betydelsen genomsyn nyttjas. Principens aktualisering medför att ett moder- och dotterbolag betraktas som en juridisk enhet, om erforderliga rekvisit uppfylls, varigenom de skadedrabbade aktieägarna från ett teoretiskt perspektiv även ses som aktieägare i dotterbolaget. Effekten av principens tillämpning är sålunda att en ”indirekt” indirekt skada faller inom paragrafens tillämpningsområde. / Whenever individual shareholders inflict an injury, due to action taken by a corporate member, they possess the right to impose liability in accordance with 29:1 1 st. 2 men. ABL. The paragraph is though associated with some difficulties when it comes to an indirect injury. The difficulties regard which rules that have to be infringed, in order for the paragraph to be applicable and thereby providing shareholders with the right to sue. Two different interpretations can hereby be identified, whereby one limited and the other one more liberal. Even if the liberal interpretation is associated with some difficulties, I find that this one should be applied. Such an application would mean that an individual shareholder acquire the right to sue, for an indirect injury, when rules protecting a third person and the corporate member’s duty of loyalty have been infringed. Legal progress has also given rise to another question, whether an “indirect” indirect injury falls within the paragraphs application. Such an injury is mostly affiliated with corporate groups where the daughter company causes a direct injury, whereby the shareholders in the mother company causes an “indirect” indirect injury. Since the shareholder here is not an owner of the daughter company, the paragraph could not be applied. A solution to this problem is, according to me, an application of the principle piercing the corporate veil, whereby the paragraphs application extends to corporate groups. The effect of the principles application is that a mother- and daughter company becomes one legal entity, if the prerequisites are fulfilled. The outcome is hereby that a shareholder in the mother company, from a theoretical perspective, also is regarded to be an owner of the daughter company. Wherefore an “indirect” indirect injury hereby falls within the scope of the paragraphs application.