Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-12-04T20:39:16Z No. of bitstreams: 1 AleidaMariaDaSilvaLima_DISSERT.pdf: 1394996 bytes, checksum: b7dfff2dc66acf246836f373fbcdae6b (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-12-06T23:54:36Z (GMT) No. of bitstreams: 1 AleidaMariaDaSilvaLima_DISSERT.pdf: 1394996 bytes, checksum: b7dfff2dc66acf246836f373fbcdae6b (MD5) / Made available in DSpace on 2017-12-06T23:54:37Z (GMT). No. of bitstreams: 1 AleidaMariaDaSilvaLima_DISSERT.pdf: 1394996 bytes, checksum: b7dfff2dc66acf246836f373fbcdae6b (MD5) Previous issue date: 2012-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A candis?ase vulvovaginal (CVV) ? uma doen?a inflamat?ria, no tecido vaginal, causada principalmente por leveduras patog?nicas de Candida albicans. E atualmente CVV ? um problema significativo na sa?de da mulher. As ? glucanas s?o polissacar?deos estruturais da parede celular da levedura Saccharomyces cerevisiae e existem v?rios relatos que demonstram o efeito imunomodulador desta estrutura em infec??es bacterianas, virais, f?ngicas e parasit?rias. Sabendo disso, o presente estudo avaliou se a ?-glucana tem atividade imunomoduladora em camundongos com candid?ase vulvovaginal sob a influ?ncia do estr?geno. Os animais foram inoculados com C. albicans, por via intravaginal, e tratados com glucana, vaginal e intraperitonealmente, e os animais do grupo de controle receberam por via intraperitoneal, solu??o salina. Os animais tratados com glucana intraperitoneal e vaginal mostraram menor n?mero de UFC, no fluido vaginal, em compara??o com animais controles. No entanto, os resultados mais marcantes foram do intraperitoneal, confirmando com o histopatol?gico, por?m sem diferen?a estatisticamente significante. Em rela??o ao influxo dos neutr?filos polimorfonucleares (PMNs), os grupos tratados com glucana mostraram maior infiltra??o desses. Al?m disso, observou-se que os animais tratados com glucana apresentaram uma maior quantidade de IFN-?, no lavado vaginal, em compara??o com o controle. Os dados sugerem que glucana pode ter uma atividade importante na prote??o contra C. albicans. / Vulvovaginal candidiasis (VVC) is an inflammatory disease, on vaginal tissue, caused mainly by pathogenic yeasts of Candida albicans. And actually, VVC is currently a significant problem in women's health. The ?-glucans are structural polysaccharides of the cell wall of the fungi Saccharomyces cerevisiae and there are several reports that demonstrate the immunomodulatory effect this structure in infections of bacterial, viral, fungal and parasitic. Knowing this, the present study evaluated whether ?- glucan has immunomodulatory activity in mice with vulvovaginal candidiasis under the influence of estrogen. The animals were inoculated with C. albicans, intravaginally, and treated with glucan, vaginally and intraperitoneally, and the control group animals received saline, intraperitoneally. The animals treated with intraperitoneal and vaginal glucan showed smaller number of the CFU, in the vaginal fluid, compared with control animals. However the results more marked was of the intraperitonel, confirmed with histopathological, but in neither case the results were statistically significant. Relative to influx of the polymorphonuclear neutrophilis (PMNs), the groups treated with glucan showed greater infiltration. Moreover, it was observed that animals treated with glucan showed a higher quantity of IFN-?, in vaginal washed, compared with the control. The data suggest that glucan may have an important activity in protection against C. albicans.

Submitted by Viviane Lima da Cunha (viviane@biblioteca.ufpb.br) on 2016-06-17T14:05:17Z No. of bitstreams: 1 arquivototal.pdf: 1836345 bytes, checksum: 07b2bb2384050a2c29d3e783883db121 (MD5) / Made available in DSpace on 2016-06-17T14:11:00Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 1836345 bytes, checksum: 07b2bb2384050a2c29d3e783883db121 (MD5) Previous issue date: 2015-05-21 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Leishmaniasis is a disease transmitted by protozoans of the Leishmania gender. A huge number of species are responsible for the infection in human beings, resulting in three clinical phenotypes. The World Health Organization – WHO classify them as neglected diseases, and encourages the search of new medical drugs or formulations that are effective and that have low toxicity to humans. All the medical drugs actually used for the treatment of the leishmaniasis present some kind of restriction such as high toxicity, severe side effects, elevated cost, parenteral administration or teratogenicity. That being said, the development of more effective and selective drugs is very important, and the identification of exclusive metabolic pathways of the parasites that can be targeted might be an interesting starting point. Medicinal plants have been a rich source to the obtaining of molecules to be therapeutically explored. The Bixa Orellana L., popularly know as “annatto”, presents different pharmacological activities that are already proven. The objective of this work was to verify the anti-leishmaniasis potential and the toxicity of the phytochemicals of the oily fraction of Bixa Orellana and bixin extracted of its seeds, aiming to verify and prove its biological activity, security and therapeutic effectiveness in order to develop a phytotherapic drug. The bixin was characterized through a nuclear magnetic resonance spectrum of ¹H and ¹³C, and the oily fraction by a gas chromatography within a mass spectrometer. It can be inferred that the bixin and the oily fraction are presented as toxic because they have shown values of LD50 of 353,64±67,54 and 285,41±35,81μg/mL, respectively, and present low toxicity against swiss mouse erythrocytes and low toxicity after acute administration of the 2000 mg/mL doses. The bixin and the oily fraction inhibited the growth of promastigote forms, generating an IC50 of 2,16 μg/mL and 1,26 μg/mL, respectively, for the Leishmania major. The Trypan blue exclusion test has shown a significant cytotoxicity of the studied substances over murine macrophages. The calculated CC50 for the murine macrophages was 59,51 μg/mL and 111,41 μg/mL, for the bixin and oily fraction, respectively, so, they are more toxic for the parasites than for the relieved macrophages. The bixin and the oily fraction did not present an in vivo anti-Leishmaniasis effect in swiss mice infected with the L. major. It can be concluded that both bixin and oily fraction presented anti-Leishmaniasis activity (leishmaniostatic and leishmanicide) against promastigote forms of L. major. / A leishmaniose é uma doença transmitida por protozoários do gênero Leishmania. Numerosas espécies são responsáveis pela infecção em humanos, resultando em três fenótipos clínicos. A OMS as classificam como doenças negligenciadas, e incentiva a busca de novos fármacos ou formulações que sejam eficazes e que tenham baixa toxicidade ao homem. Todos os fármacos atualmente em uso para o tratamento da leishmaniose apresentam restrições, como toxicidade, graves efeitos colaterais, custo elevado, administração parenteral ou teratogenicidade. Desta forma, o desenvolvimento de fármacos mais eficazes e seletivos é de suma importância, e a identificação de vias metabólicas exclusivas do parasita que possam ser usadas como alvo pode ser um ponto de partida interessante. As plantas medicinais têm sido uma rica fonte para obtenção de moléculas para serem exploradas terapeuticamente. A Bixa orellana L. popularmente conhecido como “urucum” apresenta diversas atividades farmacológicas comprovadas. O objetivo do presente estudo foi verificar o potencial antileishmania e a toxicidade dos fitoconstituintes da fração oleosa da semente de B. orellana L. e da bixina, visando comprovar sua atividade biológica, seguranças e eficácia terapêutica. A bixina foi caracterizada através de espectros de ressonância magnética nuclear de ¹H e ¹³C e a fração oleosa por cromatógrafo a gás acoplado a espectrômetro de massas. Pode-se inferir que a bixina e a fração oleosa apresentam-se como tóxicos para Artemia salina por apresentar valor de DL50, respectivamente, de 353,64±67,54 e 285,41±35,81μg/mL, baixa toxicidade frente eritrócitos de camundongos suíços e baixa toxicidade após administração aguda na dose de 2000 mg/mL.A bixina e a fração oleosa inibiram o crescimento das formas promastigotas, gerando uma IC50 de 2,16 μg/mL e 1,26 μg/mL, respectivamente, para Leishmania major. O teste de exclusão do azul de Trypan demonstrou uma significativa citotoxicidade das substâncias em estudo sobre macrófagos murinos. A CC50 calculada para os macrófagos murinos foi de 59,51 μg/mL e 111,41 μg/mL, para a bixina e fração oleosa, respectivamente, portanto, são mais tóxicas para os parasitos que para os macrófagos avaliados. A bixina e a fração oleosa não apresentaram um efeito antileishmania in vivo em camundongos suíços infectados com L. major. Pode-se concluir que a bixina e a fração oleosa apresentaram atividade antileishmania (leishmaniostática e leishmanicida) sobre formas promastigotas de L.major.

Made available in DSpace on 2014-12-17T14:16:22Z (GMT). No. of bitstreams: 1 ValeriaCRD.pdf: 561661 bytes, checksum: db7bb8f80270953b3297a614e463feeb (MD5) Previous issue date: 2003-03-21 / Infections for intestinal parasites are one of the main morbidade causes in humans and, its relationships with socioeconomic levels and hygiene conditions in countries in development are already very established. Many works, even so, they are being accomplished to elucidate the complex interactions among nutrition, these infections and answer imunol?gica, because it is seen that malnutrition commits the immunity increasing the susceptibilidade for infectious diseases and these for its time can harm the state human nutricional. It is known that sponge helm?nticos they stimulate synthesis of IgE so much policlonal as specific for the same ones and that IgA secretora, main imunoglobulina of defense of the mucous ones, can act against protozoa as the Giardia lamblia and against helmintos as Trichuris tichiura and Strongyloides stercorales. Some studies show that the malnutrition energy prot?ica influences in the production of these answers, but some authors show results divergentes. In this work it was evaluated the levels of total IgE, IgA s?rica and secretora, contagem of sanguine eosin?filos, levels of proteins s?ricas and state nutricional, in 103 children of low socioeconomic level, to discover a correlation between those and infection for enteroparasitas. They participated in the study children of both sexes, with age of 3 to 6 years, visitors of the same creche and residents in a neighborhood with precarious hygiene conditions and basic saneamento, in the city of Christmas. The obtained results showed that the faulty environmental and socioeconomic conditions favored to a high infection frequency for enteroparasitas, mainly Trichuris trichiura and Ascaris lumbricoides between the helmintos and Endolimax sleep and Gi?rdia lamblia among the protozoa. Light malnutrition without deficit prot?ico was observed in 30% of the children, which didn't also present significant deficiencies of IgA s?rica and secretora. The sponged children / Infec??es por parasitas intestinais s?o uma das principais causas de morbidade em humanos e, suas rela??es com n?veis s?cio-econ?micos e condi??es de higiene em pa?ses em desenvolvimento j? s?o bem estabelecidas. Muitos trabalhos, por?m, est?o sendo realizados para elucidar as complexas intera??es entre nutri??o, estas infec??es e resposta imunol?gica, pois ? visto que desnutri??o compromete a imunidade aumentando a susceptibilidade para doen?as infecciosas e estas por sua vez podem prejudicar o estado nutricional humano. Sabe-se que parasitas helm?nticos estimulam s?ntese de IgE tanto policlonal como espec?fica para ant?geno dos mesmos e que IgA secretora, principal imunoglobulina de defesa das mucosas, pode atuar contra protozo?rios como a Giardia lamblia e contra helmintos como Trichuris tichiura e Strongyloides stercorales. Alguns estudos mostram que a desnutri??o energ?tico prot?ica influencia na produ??o destas respostas, mas outros autores mostram resultados divergentes. Neste trabalho avaliou-se os n?veis de IgE total, IgA s?rica e secretora, contagem de eosin?filos sangu?neos, n?veis de prote?nas s?ricas e estado nutricional, em 103 crian?as de baixo n?vel s?cio-econ?mico, para se averiguar uma correla??o entre esses e infec??o por enteroparasitas. Participaram do estudo crian?as de ambos os sexos, com idade de 3 a 6 anos, freq?entadoras da mesma creche e residentes em um bairro com prec?rias condi??es de higiene e saneamento b?sico, na cidade do Natal. Os resultados obtidos mostraram que as deficientes condi??es ambientais e s?cio-econ?micas favoreceram a uma alta freq??ncia de infec??o por enteroparasitas, principalmente Trichuris trichiura e Ascaris lumbricoides entre os helmintos e Endolimax nana e Gi?rdia lamblia entre os protozo?rios. Desnutri??o leve sem d?ficit prot?ico foi observada em 30% das crian?as, as quais tamb?m n?o apresentaram defici?ncias significativas de IgA s?rica e secretora. As crian?as parasitadas apresentaram eosinofilia sangu?nea e n?veis s?ricos de IgE total elevados confirmando a importante participa??o das mesmas na resposta imune contra helmintos. Pode-se, portanto, sugerir que as crian?as apesar de poliparasitadas n?o estavam com sua resposta imune de mucosa contra parasitas, prejudicada, provavelmente por ainda n?o estarem intensamente infectados, como observado na contagem de ovos por grama de fezes e tamb?m por n?o terem seu estado nutricional gravemente comprometido

Made available in DSpace on 2014-12-17T14:16:24Z (GMT). No. of bitstreams: 1 ElaineCSF.pdf: 1327914 bytes, checksum: 0b888358d22fd2b15c2d80ebfffe69dd (MD5) Previous issue date: 2007-04-30 / Diabetes mellitus has been associated with bone metabolism alterations, such as osteopenia and osteporosis. So, the search of new anabolic agents promote bone mass gain can be important to prevent osteoporosis. The aim of this study was evaluate zinc anabolic effect over bone in diabetic and post-menopausal osteopenic models. Diabetes was induced by STZ (45mg/Kg of body weight) administration and post-menopausal by bilateral ovariectomy. Adults female Wistar rats (n=65) were divided in 5 groups: control group (n=15), ovariectomized without (n=15) and with zinc supplementation (n=10) groups, diabetic and ovarioctomized without (n=15) and with zinc supplementation (n=10) groups. Studied periods had been untill 90 days. Diabetic condition was confirmed hiperglicemic state and alterations of state with polyuria, polyphagia, polydipsia and glucosuria. Histomorphometric analysis showed that zinc supplementation increased trabecular thickness and reduced trabecular distance significantly in diabetic groups with similar values to those showed in control group. Correlation analysis of histomorphometric parameters with serum glucose concentration showed that more time in hyperglycemia more bone damage, as well as, zinc supplementation contributed to prevent this damage. Elevated serum glucose caused hyperzincuria, phosphaturia and calciuria. Zinc supplementation promoted increased levels of calcium and phosphorous ions in 90th days diabetic group. No alteration was observed by ovariectomy in mineral (Ca, P and Zn) serum and urine concentrations. Total serum Alkaline Phosphatase activity increased in diabetic groups, supplemented or not, compared with control group. However, Tartarate-Resistant Acid Phosphatase, magnesium and serum zinc did not altered in studied groups. Serum albumin was reduced only in diabetic groups. Serum creatinine was unaltered. These results support the hypotesis that zinc can be used to prevent and treat diabetic and post-menopausal osteopenia / Diabetes mellitus est? associado com altera??es do metabolismo ?sseo (osteopenia e/ou osteoporose). Identificar agentes anab?licos que promovam o ganho de massa ?ssea ? de fundamental import?ncia. Assim, o presente trabalho tem por objetivo avaliar o efeito anab?lico do zinco sobre o tecido ?sseo em modelo de osteopenia diab?tica e p?s-menopausa. O diabetes foi induzido pela administra??o de STZ (45 mg/Kg de peso corp?reo) e a p?s-menopausa pela ooforectomia bilateral. Ratas Wistar adultas (n= 65) foram divididas em 5 grupos: grupo controle (n=15), grupo ooforectomizado sem (n =15) e com zinco suplementar (n=10), grupo diab?tico e ooforectomizado sem (n=15) e com zinco suplementar (n=10). O per?odo de estudo foi de at? 90 dias. A condi??o diab?tica foi evidenciada pela hiperglicemia e pelo quadro metab?lico caracter?stico de poli?ria, polifagia, polidpsia e glicos?ria. As an?lises histomorfom?tricas mostraram que a suplementa??o com zinco promoveu um aumento da espessura e diminuiu a dist?ncia trabecular ?ssea significativamente nos grupos diab?ticos para valores pr?ximos aos dos controles e que o volume ?sseo aumentou significativamente, tanto na defici?ncia de estr?geno como nos animais com diabetes ao longo do estudo. A an?lise de correla??o entre par?metros histomorfom?tricos e glicemia evidenciaram que quanto maior o tempo de hiperglicemia maior a a??o delet?ria sobre o tecido ?sseo e que a suplementa??o com zinco minimizou esta a??o com um ganho significativo da massa ?ssea. Os grupos diab?ticos apresentaram hiperzinc?ria, fosfat?ria e calci?ria. A suplementa??o com zinco promoveu um aumento na concentra??o dos ?ons c?lcio e f?sforo s?rico para o grupo diab?tico em 90 dias. A ooforectomia n?o promoveu altera??o nas concentra??es s?ricas e urin?rias dos minerais Ca, P e Zn. A atividade da fosfatase alcalina s?rica total aumentou somente para os grupos diab?ticos, independente da suplementa??o. J? a fosfatase ?cida tartarato-resistente, o magn?sio e o zinco s?rico n?o alteraram para os grupos estudados. Apenas os grupos diab?ticos apresentaram diminui??o da albumina s?rica, embora a creatinina s?rica tenha se apresentado dentro dos valores de refer?ncia. O conjunto de resultados obtidos suportam que zinco poder? ser utilizado na preven??o e tratamento da osteopenia diab?tica e p?s-menopausa

Submitted by Automação e Estatística (sst@bczm.ufrn.br) on 2018-05-03T00:00:27Z No. of bitstreams: 1 IsabelleCristinaClementeDosSantos_DISSERT.pdf: 2438763 bytes, checksum: 4b6084ab5320a59121b13b7e45b91126 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-05-08T22:15:39Z (GMT) No. of bitstreams: 1 IsabelleCristinaClementeDosSantos_DISSERT.pdf: 2438763 bytes, checksum: 4b6084ab5320a59121b13b7e45b91126 (MD5) / Made available in DSpace on 2018-05-08T22:15:39Z (GMT). No. of bitstreams: 1 IsabelleCristinaClementeDosSantos_DISSERT.pdf: 2438763 bytes, checksum: 4b6084ab5320a59121b13b7e45b91126 (MD5) Previous issue date: 2018-03-09 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O câncer de tireoide (CT) é o tumor endócrino mais comum e corresponde a 1% de todas as neoplasias malignas. O número de casos diagnosticados vem aumentando e estima-se que surjam 212 mil novos casos anuais no mundo. Atualmente, não há nenhum estudo de predisposição genética ao CT realizado na população miscigenada do Rio Grande do Norte, local cuja indecência é elevada em comparação à média da população brasileira. Desta forma, o presente projeto busca avaliar a predisposição genética da população local em desenvolver o CT, a fim de compreender melhor a fisiopatologia desta doença e estabelecer potenciais biomarcadores de predisposição. Neste intuito, foi selecionado material histopatológico do banco de amostras do laboratório de Patologia da LNRCC para compor o grupo de estudo, e sangue periférico de doadores do banco de sangue Hemovida, como grupo controle. O DNA genômico das glândulas tireoide emblocadas em parafina e sangue periférico dos controles foram extraídos utilizando o conjunto de reagentes QIAamp® DNA FFPE Tissue e QIAamp® genomic DNA, respectivamente. A genotipagem de um painel de 84 SNPs foi executada através do espectrofotômetro de massa MALDI-TOF Sequenom® MassARRAY iPLEX Gold, em colaboração com o Centro Nacional de Genotipagem de Santiago de Compostela – Espanha. Trinta e seis SNPs mostraram diferenças significativas em suas frequências alélicas quando comparados entre os casos e controles. Destes, 23 SNPs foram associados ao risco de desenvolvimento do CT, destacando-se entre eles as variantes rs2997312 (OR= 6,33), rs7024345 (OR= 2,97), rs10788123 (OR= 2,78), rs116909374 (OR= 3,40) e rs965513 (OR= 2,91) por conferir maior risco de desenvolver CT aos pacientes. Adicionalmente, 13 SNPs se apresentaram como um fator de proteção para o não desenvolvimento desta neoplasia. Desta forma, o presente estudo, inédito na população do Rio Grande do Norte, sugere a associação destes SNPs como biomarcadores de predisposição de CT nesta população. / Thyroid cancer (CT) is the most common endocrine tumor and accounts for 1% of all malignancies. The number of cases diagnosed has been increasing and it is estimated that 212 thousand new cases will appear each year in the world. Currently, there is no study of genetic predisposition to CT performed in the admixture population of Rio Grande do Norte, a place where its incidence is high compared to the Brazilian average. In this way, the present project aims to evaluate the genetic predisposition of the local population to develop the CT, to better understand the pathophysiology of this disease and to establish potential biomarkers of predisposition. To this purpose, histopathological material was selected from the sample bank of the LNRCC Pathology Laboratory to compose the study group, and venous blood from donors from Hemovida blood bank as a control group. Genomic DNA from thyroid gland paraffin blocks and controls blood samples were extracted using the QIAamp® DNA FFPE Tissue kit and QIAamp® genomic DNA kit, respectively. The genotyping of a panel of 84 SNPs was performed using the MALDI-TOF Sequenom® MassARRAY iPLEX Gold Mass Spectrophotometer, in collaboration with the National Center for Genotyping of Santiago de Compostela - Spain. Thirty-six SNPs showed significant differences in their allelic frequencies when compared cases and controls. Of these, 23 SNPs were associated with risk of developing CT, mainly the variants rs2997312 (OR= 6,33), rs7024345 (OR= 2,97), rs10788123 (OR= 2,78), rs116909374 (OR= 3,40) and rs965513 (OR= 2,91) once they confer greater risk to patients. Additionally, 13 SNPs presented as a protective factor for the non-development of this neoplasia. Thus, the present study, unprecedented in the population of Rio Grande do Norte, suggests the association of these SNPs as biomarkers of CT predisposition in this population.

Made available in DSpace on 2015-03-03T14:03:53Z (GMT). No. of bitstreams: 1 MariaMCA_DISSERT.pdf: 1294142 bytes, checksum: 64dee49ca0e00aab7f3d623528da9204 (MD5) Previous issue date: 2006-05-17 / Considering that osteopenia and osteoporosis are diabetes mellitus complications, and that tamoxifen (TAM) is an anti-estrogenic drug used in breast cancer treatment, this drug may have a beneficial action preventing accentuaded bone loss associated to diabetes. Female Wistar rats (n=60) weighting 180-250g were divided in four groups: Group C, control animals (n=5); Group T, animals treated with TAM (n=5); Group D, diabetic animals (n=5); and Group DT, diabetic animals treated with TAM (n=5). Oestrus cycle was evaluated before the beggining of experimental period to select the animals with regular cycle. This evaluation continued throughout the study period and for all studied groups. Diabetes was induced by a intra perithoneal injection of streptozotocin (STZ) in a concentration of 45 mg/Kg of body weight. Those animals with serum glicose levels 250 mg/dL were considered diabetics. Animals were sacrificed in the periods of 30, 60 and 90 days after diabetes onset. Left femur histomorphometric measurements and serum biochemical analysis (glycemia, alkaline phosphatase, tartaric-resistant acid phosphatase, calcium, phosphorous, magnesium, total proteins, albumin, globulins, urea and creatinine) were done. Histomorphometric results showed a progressive bone loss in Group D animals when compared to those from Group C all over the experimental period, becoming accentuaded in the 90 days period. In relation to Groups T and DT, values approcimated to those obtained for control group were found during the whole period of study. Those data may indicate a bone mass recovery or a diminished bone loss due to diabetes when animals were treated with TAM. During the whole experimental period animals of groups D and DT maintained glycemic levels above 250 mg/dL whereas animals of groups C and T maintained those levels below 150mg/dL. Alkaline phosphatase activity was increased in all study periods for groups D and DT when compared to group C animals over the 90 days period. Tartarate-resistant acid phosphatase activity was showed unaltered in all periods of study and for all groups. Calcium and magnesium results were also unaltered, maintaining reference levels for all groups in all experimental periods. Phosphorous levels were increased in groups D and DT when compared to groups C and T in the 30 days period. However no difference was found in the periods of 60 and 90 days for this test. No difference was found for total proteins levels for groups C, T, D and DT over the study period. Albumin levels were reduced in DT group in the 60 days period and in D and DT groups in the 90 days period. Urea levels were significantly increased in the 30, 60 and 90 days study periods in groups D and DT when compared to groups C and T. Creatinine results showed a significantly increase in the 90 days period for groups D and DT when compared to groups C and T, and maintaining unaltered in the 30 and 60 days periods. These results suggest that the treatment with TAM may reduce bone loss caused by diabetes mellitus / A influ?ncia do tratamento com Tamoxifeno (TAM) na densidade mineral ?ssea foi estudada em ratos Wistar f?meas diab?ticos. Foram utilizados 60 ratos Wistar f?meas (180-250g), sendo divididos em quatro grupos: controle C (n=5), tratado com TAM T (n=5), diabete D (n=5) e diabete tratado com TAM DT (n=5). Foi feita avalia??o do ciclo estral durante 15 dias antes do in?cio do experimento para selecionar os animais com ciclos regulares e durante os per?odos de 30, 60 e 90 dias, em todos os grupos estudados. O diabete foi induzido com inje??o intraperitoneal de estreptozotocina STZ (45 mg/Kg) e os animais foram sacrificados em per?odos de 30, 60 e 90 dias ap?s a instala??o do diabete melito (glicemia ?? 250 mg/dL). Foram realizadas medidas histomorfom?tricas dos f?mures esquerdos e an?lises bioqu?micas de glicose, fosfatase alcalina, fosfatase ?cida tartarato resistente, c?lcio, f?sforo, magn?sio, prote?nas totais, albumina, globulina, ur?ia e creatinina em amostras de soro. Os resultados histomorfom?tricos mostraram uma perda progressiva de massa ?ssea, nos f?mures dos animais do grupo D comparados ao grupo C em todos os per?odos estudados. O grupo T se manteve sem altera??o nos per?odos e em rela??o ao grupo DT observou-se que os valores da an?lise histomorfom?trica mantiveram-se pr?ximos ao grupo C em todos os per?odos estudados, indicando um aumento de massa ?ssea induzido pelo uso de TAM. Durante todo o experimento a glicemia dos animais D e DT manteve-se sempre acima de 250mg/dL e do grupo C e T inferior a 150mg/dL. A atividade da fosfatase alcalina esteve aumentada em todos os per?odos estudados para os grupos D e DT quando comparados ao grupo C. O f?sforo nos grupos D e DT encontrou-se aumentado quando comparado ao grupo C e T no per?odo 30 dias. J? nos per?odos 60 e 90 dias n?o foram observadas altera??es significativas nos grupos estudados. A albumina encontrou-se diminu?da no grupo DT per?odo 60 dias e nos grupos D e DT no per?odo 90 dias. Em rela??o a ur?ia encontramos aumento significativo nos per?odos 30, 60 e 90 dias para os grupos D e DT em rela??o aos grupos C e T. A creatinina teve aumento significativo no per?odo 90 dias para os grupos D e DT quando comparados ao C e T, mantendo-se inalterada em todos os grupos nos per?odos 30 e 60 dias. Para a fosfatase ?cida tartaratoresistente, c?lcio, magn?sio e prote?nas totais n?o houve altera??o nos per?odos estudados para todos os grupos. Os resultados obtidos confirmam a a??o ben?fica de TAM no osso indicando um aumento de massa ?ssea em ratos wistar diab?ticos tratados com TAM

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-25T22:57:38Z No. of bitstreams: 1 GabrielaDinizFonseca_DISSERT.pdf: 1765873 bytes, checksum: 5a2297e7f8e6f38144397041114ffaff (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-27T23:04:29Z (GMT) No. of bitstreams: 1 GabrielaDinizFonseca_DISSERT.pdf: 1765873 bytes, checksum: 5a2297e7f8e6f38144397041114ffaff (MD5) / Made available in DSpace on 2016-04-27T23:04:29Z (GMT). No. of bitstreams: 1 GabrielaDinizFonseca_DISSERT.pdf: 1765873 bytes, checksum: 5a2297e7f8e6f38144397041114ffaff (MD5) Previous issue date: 2015-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / A quitosana ? um co-pol?mero biocompat?vel e biodegrad?vel amplamente usado em sistemas de libera??o de f?rmacos. A co-reticula??o da quitosana com sulfato de s?dio e genipina para forma??o de sistemas particulados ? capaz de tornar estes mais resistentes ao pH ?cido e modificar a cin?tica de libera??o de f?rmacos para a via oral. A triancinolona ? um glicocortic?ide de ampla aplica??o terap?utica por suas a??es antiinflamat?ria e imunossupressora. Os sistemas particulados foram preparados por co-reticula??o e caracterizadas quanto ao tamanho de part?cula, PDI, potencial zeta, grau de reticula??o, taxa de encapsula??o, MEV, espectroscopia de infravermelho, an?lise t?rmica, cin?tica de libera??o e estudo em c?lulas tumorais. Inicialmente foram preparadas nanopart?culas com sulfato de s?dio, sem genipina, contendo triancinolona, avaliando a propor??o do volume das fases f?rmaco/pol?mero, concentra??o de sulfato de s?dio e polissorbato 80, tempo e modo de imers?o das fases. O sistema definido para a reticula??o com a genipina apresentou tamanho de 312,20 ? 5,70 nm, PDI 0,342 ? 0,013 e potencial zeta 20,18 ? 2,28 mV. A genipina foi introduzida no sistema em diferentes concentra??es (0,5; 1,0 e 2,0 mM) e tempos de reticula??o (3, 6, 12 e 24 h). Na an?lise do tempo de reticula??o com genipina 0,5 mM, os sistemas obtiveram tamanho de 235,1 a 334,4 nm, PDI de 0,321 a 0,392 e potencial zeta de 20,92 a 30,39 mV, com grau de reticula??o que variou de 14 a 30 %. As nanopart?culas sem genipina, 6 h e 24 h de reticula??o foram escolhidas para a secagem por spray-drying, formando micropart?culas de nanopart?culas. A an?lise por micrografia eletr?nica de varredura revelou que as micropart?culas apresentaram morfologia esf?rica. A taxa de encapsula??o foi de 75 ? 2,3% usando metodologia CLAE validada. A caracteriza??o dos sistemas por infravermelho evidenciou as intera??es entre os componentes da formula??o. A an?lise t?rmica mostrou que os sistemas com maior grau de reticula??o, apresentaram uma maior estabilidade t?rmica. Na cin?tica de libera??o, o aumento do grau de reticula??o foi capaz de diminuir a velocidade de libera??o da triancinolona. Nos estudos com c?lulas HepG2 e HT-29 os sistemas microparticulados contendo triancinolona e genipina com tempo de reticula??o de 24 h apresentaram um potencial para atividade antitumoral nas c?lulas de linhagem hep?ticas HepG2. Com isso, um novo sistema de libera??o a base de quitosana obtido por co-reticula??o com sulfato de s?dio e genipina contendo triancinolona foi obtido com potencial antitumoral hep?tico. / Chitosan is a polymer biocompatibility and biodegradability widely used in drug delivery systems. The co-crosslinking of chitosan with sodium sulfate and genipin, to form particulate systems is related of making them more resistant to acidic pH and to modulate the release kinetics for the oral route. Triamcinolone is a glucocorticoid with anti-inflammatory and immunosuppressive actions. The nanoparticles were prepared by co-crosslinking and characterized for particle size, PDI, zeta potential, crosslinking degree, encapsulation rate, morphology, infrared spectroscopy, thermal analysis, release kinetics and cells studies. The nanoparticles were prepared initially without genipin with sodium sulphate and the particles parameters were monitored in function of different ratio of drug / polymer, different concentrations of sodium sulfate and polysorbate 80 and the drip mode of crosslinkers on polymers. After optimizing conditions, the chosen system parameters without genipin included mean diameter of 312.20 ? 5.70 nm, PDI 0.342 ? 0.013 and zeta potential of 20.18 ? 2.28 mV. The genipin was introduced into the system analyzing different concentrations (0.5, 1.0 and 2.0 mM) and crosslinking times (3, 6, 12 and 24 h). Evaluating crosslinking time with genipin (0.5 mM) it was showed that varying the genipin reaction time the systems size ranged from 235.1 to 334.4 nm, the PDI from 0.321 to 0.392 and zeta potential 20.92 to 30.39 mV. The crosslinking degree that coud vary from 14 to 30 %. Nanoparticles without genipina, 6 h and 24 h crosslinking time were dried by spray-drying method. Analysis by scanning electron micrograph (SEM) revealed that the microparticles showed spherical morphology. The encapsulation rate was 75 ? 2.3 % using validated HPLC methodology. The infrared analysis showed chemical interactions between the components of the formulation. Thermal analysis showed that systems with a higher degree of crosslinking had a higher thermal stability. On release kinetics, increasing the degree of crosslinking was able to decrease the concentration and rate of release of triamcinolone. In studies with liver cancer cells (HepG2) and colon (HT-29), the microparticulate prepared with triamcinolone and 24 h of crosslinking with genipin showed a potential for antitumor activity in hepatic cell line HepG2. Therefore, a new delivery system for triamcinolone on polymeric nanoparticles of chitosan cocrosslinked with genipin and sodium sulfate was obtained with hepatic antitumor potential.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-10-26T20:21:40Z No. of bitstreams: 1 JoseQueirozFilho_DISSERT.pdf: 1098383 bytes, checksum: 472cf48097095810703203b62b883630 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-12-20T17:53:06Z (GMT) No. of bitstreams: 1 JoseQueirozFilho_DISSERT.pdf: 1098383 bytes, checksum: 472cf48097095810703203b62b883630 (MD5) / Made available in DSpace on 2016-12-20T17:53:06Z (GMT). No. of bitstreams: 1 JoseQueirozFilho_DISSERT.pdf: 1098383 bytes, checksum: 472cf48097095810703203b62b883630 (MD5) Previous issue date: 2006-04-26 / A candid?ase vulvovaginal (CVV) ? uma das infec??es mais comuns na pr?tica cl?nica de um ginecologista, e o controle dessa patologia ? realizado por v?rios mecanismos de defesa, onde a resposta imune celular parece ser fundamental. Com a finalidade de investigar a resposta imune celular em mulheres com e sem CVV, foram avaliadas c?lulas do sangue perif?rico da mucosa vaginal, concentra??es de IgE s?rica total e espec?fica para Candida albicans de 60 mulheres sendo 40 com CVV e 20 como grupo controle, na faixa et?ria de 10 aos 60 anos atendidas no servi?o de ginecologia do complexo de sa?de HAPVIDA em Natal (RN) no per?odo de janeiro a mar?o de 2006. As coletas de sangue perif?rico foram realizadas por pun??o venosa e a contagem celular de cada paciente foi realizada por automa??o e contagem manual em esfrega?os sanguineos corados por Giemsa. A quantifica??o de linf?citos TCD4+ e TCD8+ foi realizada por citometria de fluxo e os esfrega?os vaginais foram preparados com raspado da parede vaginal lateral, as l?minas foram fixadas imediatamente em ?lcool absoluto e coradas posteriormente com hematoxilina-eosina, foram analisados 10 campos microsc?picos por l?minas em objetivas 10X e 40X. As concentra??es de IgE total s?rica e espec?fica para Candida albicans foram obtidas por quimiluminesc?ncia e fluoroimunoensaio. No sangue perif?rico e no raspado vaginal estudou se as c?lulas tipo neutr?filos, linf?citos, eosin?filos e mon?citos . A an?lise estat?stica foi feita utilizando se o teste exato de Fisher e teste n?o param?trico de Kruskal-Wallis, Mann-Whitney e o teste de correla??o de Spermann. No grupo de mulheres com CVV a an?lise celular no sangue perif?rico houve predomin?ncia de eosin?filos (m?dia: 302,60) sendo esse achado significativo com p=0,037 em rela??o ao grupo controle. O estudo das c?lulas nos raspados vaginais demonstrou diferen?as significativas para neutr?filos (m?dia: 230.70) e linf?citos (m?dia: 11,50) com signific?ncia de P=0,0001. Com rela??o a quantifica??o de linf?citos TCD4+ e TCD8+ n?o houve significado estat?stico. As concentra??es de IgE total s?rica e espec?fica n?o apresentaram significado estat?stico e para ambos grupos os resultados encontrados foram semelhantes nas diferentes metodologias aplicadas. Ao correlacionar as concentra??es de IgE total s?rica (m?dia=308,83) com eosin?filos no sangue perif?rico de mulheres com CVV apresentou um ?ndice de correla??o de 0,25 considerado baixo. / Vulvovaginal candidiasis (VVC) is one of the most common infections in the clinical practice of a gynecologist. and control of this disease is carried out by various defense mechanisms, where the cellular immune response appears to be fundamental. In order to investigate the cellular immune response in women with and without CVV, Peripheral blood cells of vaginal mucosa were evaluated, the concentrations of total and specific IgE to Candida albicans, 60 women and 40 with VVC and 20 as a control group, aged 10 to 60 years years served in the gynecology service Hapvida health complex in Natal (RN) in the period January-March 2006. Peripheral blood samples were taken by venipuncture and cell count of each patient was performed by automation and manual counting in blood smears stained with Giemsa. Quantification of CD4 + and CD8 + lymphocytes was performed by flow cytometry Vaginal smears were prepared with shaved lateral vaginal wall, The slides were fixed immediately in absolute ethanol and then stained with hematoxylin-eosin, 10 were analyzed by microscopic fields blades eyepiece 10X and 40X objective. The serum IgE concentrations and total specific for Candida albicans, They were obtained by chemiluminescence and fluoroimmunoassay. In peripheral blood and vaginal shaved studied cell type cell neutrophils, lymphocytes, eosinophils and monocytes. Statistical analysis was performed using the Fisher exact test and nonparametric Kruskal-Wallis, Mann-Whitney and Spearman correlation test In the group of women with VVC cell analysis in peripheral blood eosinophil predominated, (average 302.60) and this significant finding with p = 0.037 in the control group. . The cell study the cells in vaginal scrapings showed significant differences in neutrophils neutrophils, (average 230.70) and lymphocytes (average 11.50) with significance of p = 0.0001. .With Respect to quantification of CD4 + T lymphocytes and CD8 + there was no statistical significance. IgE serum concentrations and specific overall did not show statistical significance and and both groups the results were similar in the different methodologies. By relating the total serum IgE concentrations (mean = 308.83), with eosinophils in the peripheral blood of women with VVC had a 0.25 considered low correlation index.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-13T19:44:50Z No. of bitstreams: 1 RayllanDeOliveiraRodrigues_DISSERT.pdf: 1576056 bytes, checksum: 9d1ce8b10b111bf188478ab4f15607ca (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-02-14T21:27:05Z (GMT) No. of bitstreams: 1 RayllanDeOliveiraRodrigues_DISSERT.pdf: 1576056 bytes, checksum: 9d1ce8b10b111bf188478ab4f15607ca (MD5) / Made available in DSpace on 2017-02-14T21:27:05Z (GMT). No. of bitstreams: 1 RayllanDeOliveiraRodrigues_DISSERT.pdf: 1576056 bytes, checksum: 9d1ce8b10b111bf188478ab4f15607ca (MD5) Previous issue date: 2016-01-26 / A esp?cie Kalanchoe brasiliensis Cambess, pertencente ? fam?lia Crassulaceae, apresenta em sua composi??o qu?mica flavonoides, polissacar?deos e ?cido asc?rbico. Esses ativos s?o comumente utilizados em formula??es cosm?ticas com propriedades antioxidantes, hidratantes e antienvelhecimento. Essa pesquisa teve como objetivos o desenvolvimento de emuls?es hidratantes aditivadas com o extrato hidroetan?lico de folhas de Kalanchoe brasiliensis e avaliar a efic?cia cl?nica hidratante das mesmas. O extrato obtido foi avaliado quanto ?s concentra??es de a??cares totais, prote?nas e polifenois totais. Foram desenvolvidas formula??es com diferentes concentra??es de Sodium Acrylates/ Beheneth-25 Methacrylate Crosspolymer (and) Hydrogenated Polydecene (and) Lauryl Glucoside (Novemer? EC-2), adicionadas ao gel de Acrylates/C10-30 Alkyl Acrylate Crosspolymer (Carbopol? Ultrez-20). As formula??es foram aditivadas ou n?o de 0,5% (p/p) do extrato vegetal em estudo e submetidas aos testes de estabilidade preliminar e acelerada. No estudo de estabilidade acelerada, as amostras foram estocadas em diferentes temperaturas durante 90 dias. As caracter?sticas organol?pticas, valor de pH e comportamento reol?gico foram avaliados. A formula??o contendo 3,0% Novemer? EC-2 e 0,4% de Carbopol? Ultrez-20 aditivada com 0,5% de extrato hidroetan?lico de K. brasiliensis foi considerada est?vel. A efic?cia cl?nica foi avaliada por meio das metodologias de capacit?ncia e da perda de ?gua transepidermal. A formula??o contendo o extrato comparado ao ve?culo promoveu aumento estatisticamente significativo do conte?do h?drico do estrato c?rneo da pele e diminui??o da perda de ?gua transepidermal promovendo efeito barreira at? cinco horas ap?s uma ?nica aplica??o da formula??o. A emuls?o desenvolvida com 0,5% do extrato hidroetan?lico das folhas de K. brasiliensis, apresentou efic?cia hidratante atuando possivelmente pelos mecanismos de oclus?o e umecta??o, demonstrando potencial uso como produto cosm?tico hidratante. / Kalanchoe brasiliensis Cambess specie, belonging to the Crassulaceae family, presents flavonoids, polysaccharides and ascorbic acid in its chemical composition. These compounds are generally used in cosmetics formulations as antioxidants, moisturizers and anti-aging properties. This research aimed to development moisturizing emulsions add or not with hydroethanolic extract of the Kalanchoe brasiliensis leaves and evaluated its moisturizing clinical efficacy. Formulations have been developed with different concentrations of Sodium Acrylates/ Beheneth-25 Methacrylate Crosspolymer (and) Hydrogenated Polydecene (and) Lauryl Glucoside (Novemer?EC-2 polymer), incorporated to Acrylates/C10-30 Alkyl Acrylate Crosspolymer (Carbopol? Ultrez-20). The formulations were added or not with plant extract 0.5% (w/w) and subjected to accelerated stability tests. In accelerated stability study, the samples were stored at different temperatures for 90 days. The organoleptic characteristics, pH and rheological behavior were evaluated. The formulation containing 3.0% Novemer?EC-2 and 0.4% Carbopol? Ultrez-20 added with hydroethanolic extract of K. brasiliensis 0.5% was considered stable. Moisturizing clinical efficacy was evaluated by capacitance and transepidermal water loss methodologies. The formulation containing extract compared to vehicle promoted increase significantly statistical of the water content of the stractum corneum and decrease of transepidermal water loss, promoting barrier effect until five hours after single application of the formulation. The emulsion developed with 0.5% of hydroethanolic extract of K. brasiliensis leaves presented moisturizing efficacy acting possibly by occlusion and humectation mechanisms, demonstrating potential use as a moisturizing cosmetic product.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-20T21:49:24Z No. of bitstreams: 1 AlannaDeSousaRodrigues_DISSERT.pdf: 996440 bytes, checksum: d75b3da15e011ba5988e8e2aad479a21 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-23T18:33:46Z (GMT) No. of bitstreams: 1 AlannaDeSousaRodrigues_DISSERT.pdf: 996440 bytes, checksum: d75b3da15e011ba5988e8e2aad479a21 (MD5) / Made available in DSpace on 2017-03-23T18:33:46Z (GMT). No. of bitstreams: 1 AlannaDeSousaRodrigues_DISSERT.pdf: 996440 bytes, checksum: d75b3da15e011ba5988e8e2aad479a21 (MD5) Previous issue date: 2003-08-29 / Embora o aumento na concentra??o de Esp?cies Reativas de Oxig?nio (Eros), devido ao diabetes mellitus, pode alterar o metabolismo normal do osso, acarretando assim a osteopenia. Uma suplementa??o com c?lcio, f?sforo e vitamina E pode ter a??o ben?fica e prevenir as complica??es associadas ao estado diab?tico. Foram utilizados ratos Wistar machos (n=125), peso corporal 250-350g, sendo divididos em tr?s grupos: grupo 1- controles (n=45) grupo 2- diab?ticos experimentais (n=45), e grupo 3 - diab?ticos suplementados (n=35) em c?lcio, f?sforo e vitamina E. O diabetes experimental foi induzido por estreptozotocina (STZ) (45 mg/Kg) e sacrificados em per?odos de 1 e 5 dias ap?s a indu??o e 5, 30, 60 e 90 dias ap?s a instala??o do diabetes mellitus. Animais com glicemia ? 250 mg/dL foram considerados diab?ticos. Realizaram-se an?lises de Subst?ncias Reativas ao ?cido Tiobarbit?rico (SRAT) e Glutationa (GSH) em sangue e c?rebro, medidas histomorfom?tricas dos f?mures esquerdos, al?m de dosagens bioqu?micas de fosfatase alcalina, fosfatase ?cida, fosfato, magn?sio, c?lcio ioniz?vel, c?lcio total, creatinina e albumina em amostras de soro e glicose, fosfato, magn?sio, creatinina e clearence de creatinina em amostras de urina dos animais. As SRAT no plasma e c?rebro, apresentaram-se significativamente elevadas no grupo 2 em rela??o ao grupo 1, e diminu?da para o grupo 3, no per?odo de 90 dias. O conte?do de GSH em c?rebro, no per?odo de 90 dias, diminuiu no grupo 2 e aumentou no grupo 3, enquanto em sangue n?o foram observadas altera??es. Os resultados histomorfom?tricos mostraram uma perda em m?dia de 50% de osso trabecular avaliadas pela dist?ncia entre as trab?culas, espessura das trab?culas e volume ?sseo trabecular, nos animais diab?ticos experimentais em rela??o aos controles e, com a suplementa??o, essa perda diminuiu significativamente alcan?ando valores pr?ximos aos encontrados no grupo controle. A atividade da fosfatase alcalina apresentou valores superiores para os grupos 2 e 3 em rela??o ao grupo 1 e a fosfatase ?cida aumentou significativamente nos grupos 2 e 3, em rela??o aos controles, no per?odo de 90 dias. As concentra??es s?ricas de fosfato, magn?sio, c?lcio ioniz?vel, c?lcio total e creatinina, n?o apresentaram altera??es uma vez que os resultados est?o dentro dos valores de intervalos de refer?ncia. A albumina s?rica mostrou-se diminu?das para o grupo 2 e 3. A an?lise da urina mostrou glicos?ria significativa para os grupos 2 e 3 em rela??o ao grupo 1. Em rela??o a fosfat?ria observou-se um aumento significativo principalmente para o grupo 3 e em rela??o ao clearence de creatinina n?o foram observadas diferen?as significativamente para o grupo 2 e 3. Os resultados observados sugerem que a suplementa??o de c?lcio, f?sforo e vitamina E diminuiu as altera??es oxidativas causadas pelo diabetes, al?m de ter exercido a??o ben?fica sobre o metabolismo ?sseo, diminuindo a perda ?ssea que ocorre durante esta s?ndrome. / The increase of EROs concentration, through diabetes mellitus, can alter the normal bone metabolism, causing osteopenia. A calcium, fosforum and vitamin E supplementation, can be a very good action in bone. In thi study were used male Wistar rats (n=125), weight ranging from 250 ? 350g, and were divided in three groups: group 1 control (n=45) group 2 experimental diabetic (n=45) and group 3 supplementadies diabetic (n=35) in calcium, fosforum and vitamin E. The animals were inuced with streptozotocin (45mg/Kg ) and killed in periods of 1 to 5 days after the indution e 5, 30, 60 and 90 days after installation of experimental diabetes mellitus. Animals with glycemia ? 250 mg/ dL was considered diabetics.In each period was realized determinations of Thiobarbituric Acid Reactive Substances and levels of reduced glutathione in blood and brain, histomorfometric measurement of left fermurs, and dosages of alkaline phosphatase, acid phosphatase, ionizable calcium, total calcium, phosphorus, magnesium, glycemia, creatinin and albumin in serum and glucose, phosphorus, magnesium, creatinine and clearence of creatinin in urine. Thiobarbituric Acid Reactive Substances at brain and plasma, choose higher at group 2 in relation group 1, and reduced in group 3, in period of 90 days. The levels of reduced glutathione in brain and blood, at 90 days, was decreased in group 2 and increased at group 3. The histomorphometric results show a less in middle of 50% in trabecular bone volume analysed per distance between the trabeculaes thickness of trabeculae and bone quantity in experimental diabetic animals in comparison to the control group and for controls group and, supplemented animals this lose reduced very much arriving sizes near found in control group. The activity of alkaline phosphatase showed superior values in relation of groups 2 and 3, about comparing group 1 and acid phosphatase increased higher in groups 2 and 3, about all controls, in a 90 days. The serum concentration of phosphorus, magnesium, ionazle calcium, total calcium and creatinine, didn?t show alterations, and the results are well if compare the reference result. The albumin serum showed less to group 2 and 3. The urine analyse showed significative glycos?ria to groups 2 and 3 if compared with group 1. In relation phosphatury, observed a significative increase principally to group 3 and creatinine clearance relation wasn?t observed differences to group 2 and 3. The results observed suggest that calcium, phosphorus and vitamin E supplementation, decreased the oxidative alterations which the diabetics were the cause, and helped bone metabolism, decreasing the lose bone that ocurr in this syndrome