A construção do paradigma socioambiental é resultado da compreensão de que não é possível a proteção isolada, implicando o cuidado conjunto. O ser humano, ente integrante da biodiversidade, está amparado pelas legislações que lhe são próprias, assim como pelas legislações ambientais. O genoma humano é próprio de cada indivíduo e o distingue dos demais entes da mesma espécie. O desenvolvimento do Projeto Genoma Humano possibilitou o acesso e o uso das informações genéticas. A evolução da ciência deve ser regulamentada para evitar a utilização indevida das informações genéticas, assim como para que os benefícios proporcionados por essa evolução sejam acessíveis a toda população. O ser humano não pode sofrer discriminação em razão da sua carga genética. O acesso às informações contidas nos genes deve ocorrer unicamente para melhorar a saúde dos indivíduos. O consentimento informado é imprescindível para acesso e uso das informações genéticas. A bioinformática possibilita a descoberta das funções de cada gene específico. A farmacogenômica, por sua vez, proporciona o tratamento e a cura de doenças de acordo com a carga genética de cada indivíduo. Os biorepositórios e os biobancos são importantes para conservar o material genético destinado à pesquisa, bem como a ser utilizado no futuro tratamento médico do próprio doador. As pesquisas genéticas devem ser conduzidas de forma transparente e regulada a fim de evitar a detenção do biopoder. O acesso do genoma humano pode permitir a manipulação desse com finalidades bioterroristas de modo a atingir à população em geral ou a determinado grupo específico. Alguns países já estão patenteando os genes, muito embora sejam considerados descoberta e não invenção. A não permissão do patenteamento de genes no Brasil deixa o país em desvantagem quanto aos demais que permitem, pois futuramente, nosso país, poderá ter que pagar royalties pela utilização dos genes já patenteados no desenvolvimento de pesquisas genéticas. Todas essas situações demonstram a importância de proteger o genoma humano para que as atuais e futuras gerações não tenham sua carga genética alterada. A criação de uma legislação nacional e, principalmente, internacional é indispensável. / Submitted by Marcelo Teixeira (mvteixeira@ucs.br) on 2014-05-29T18:15:58Z No. of bitstreams: 1 Dissertacao Monica Souza Liedke.pdf: 1347371 bytes, checksum: 041ecbd172b0c26294c008a919ffc003 (MD5) / Made available in DSpace on 2014-05-29T18:15:58Z (GMT). No. of bitstreams: 1 Dissertacao Monica Souza Liedke.pdf: 1347371 bytes, checksum: 041ecbd172b0c26294c008a919ffc003 (MD5) / The build of the socio-environment paradigm is resultant from the comprehension that isolated protection is not possible, implying collective care. The human being, as a biodiversity integrant, is supported by his laws, but also by environment laws. The human genome is unique for each individual and distinguishes itself from the others beings of the same species. The development of The Human Genome Project made possible the access to and the use of genetic information. Science evolution must be ruled to avoid improper use of genetic information, but also for granting universal access to it. The human being must not be discriminated by its genetic information. Genetic information access should only be for individual health improvement. Informed assent is essential for the access and the use of this information. Pharmacogenomics, in its turn, provides treatment and cure for diseases in agreement with every individual genetic information. The biorepositories and biobanks are important to preserve genetic material destined to research, such as future use in the medical treatment of the donor. Genetic researches must be lead in a clear and ruled form in order to avoid retention of biopower. The access to the human genome can permit its manipulation with bioterrorist intents of reaching general population or a specific group. Some countries are already patenting the genes, although they are considered findings and not inventions. The non permission of gene patenting in Brazil put the country in disadvantage with the others that permit, because, in the future, our country could pay royalties for the already patented in the development of genetic researches. All these situations show the importance of protecting human genome for this and future generations. The creation of national and, mainly, international laws is indispensable.

A construção do paradigma socioambiental é resultado da compreensão de que não é possível a proteção isolada, implicando o cuidado conjunto. O ser humano, ente integrante da biodiversidade, está amparado pelas legislações que lhe são próprias, assim como pelas legislações ambientais. O genoma humano é próprio de cada indivíduo e o distingue dos demais entes da mesma espécie. O desenvolvimento do Projeto Genoma Humano possibilitou o acesso e o uso das informações genéticas. A evolução da ciência deve ser regulamentada para evitar a utilização indevida das informações genéticas, assim como para que os benefícios proporcionados por essa evolução sejam acessíveis a toda população. O ser humano não pode sofrer discriminação em razão da sua carga genética. O acesso às informações contidas nos genes deve ocorrer unicamente para melhorar a saúde dos indivíduos. O consentimento informado é imprescindível para acesso e uso das informações genéticas. A bioinformática possibilita a descoberta das funções de cada gene específico. A farmacogenômica, por sua vez, proporciona o tratamento e a cura de doenças de acordo com a carga genética de cada indivíduo. Os biorepositórios e os biobancos são importantes para conservar o material genético destinado à pesquisa, bem como a ser utilizado no futuro tratamento médico do próprio doador. As pesquisas genéticas devem ser conduzidas de forma transparente e regulada a fim de evitar a detenção do biopoder. O acesso do genoma humano pode permitir a manipulação desse com finalidades bioterroristas de modo a atingir à população em geral ou a determinado grupo específico. Alguns países já estão patenteando os genes, muito embora sejam considerados descoberta e não invenção. A não permissão do patenteamento de genes no Brasil deixa o país em desvantagem quanto aos demais que permitem, pois futuramente, nosso país, poderá ter que pagar royalties pela utilização dos genes já patenteados no desenvolvimento de pesquisas genéticas. Todas essas situações demonstram a importância de proteger o genoma humano para que as atuais e futuras gerações não tenham sua carga genética alterada. A criação de uma legislação nacional e, principalmente, internacional é indispensável. / The build of the socio-environment paradigm is resultant from the comprehension that isolated protection is not possible, implying collective care. The human being, as a biodiversity integrant, is supported by his laws, but also by environment laws. The human genome is unique for each individual and distinguishes itself from the others beings of the same species. The development of The Human Genome Project made possible the access to and the use of genetic information. Science evolution must be ruled to avoid improper use of genetic information, but also for granting universal access to it. The human being must not be discriminated by its genetic information. Genetic information access should only be for individual health improvement. Informed assent is essential for the access and the use of this information. Pharmacogenomics, in its turn, provides treatment and cure for diseases in agreement with every individual genetic information. The biorepositories and biobanks are important to preserve genetic material destined to research, such as future use in the medical treatment of the donor. Genetic researches must be lead in a clear and ruled form in order to avoid retention of biopower. The access to the human genome can permit its manipulation with bioterrorist intents of reaching general population or a specific group. Some countries are already patenting the genes, although they are considered findings and not inventions. The non permission of gene patenting in Brazil put the country in disadvantage with the others that permit, because, in the future, our country could pay royalties for the already patented in the development of genetic researches. All these situations show the importance of protecting human genome for this and future generations. The creation of national and, mainly, international laws is indispensable.

Etiquetas de sequências expressas (ESTs) são fundamentais para a identificação de genes no genoma humano e para definir características de expressão gênica. Neste trabalho, descrevemos uma nova abordagem para a geração de bibliotecas de cDNA, utilizando iniciadores arbitrários para a produção, por PCR, de mini-bibliotecas a partir de mRNA derivado de câncer de mama. Clones destas bibliotecas foram sequenciadas para gerar 6029 ESTs. Utilizando esta abordagem, foi possível observar uma significante normalização das diferentes sub-populações de mRNA e amplificação preferencial de porções centrais dos genes. Análise bioinformática destas sequências mostra que 3.350 ESTs (56%) tem similaridade significante a sequências de DNA e/ou cDNA já conhecidas (sequências anotadas) descritas em diferentes organismos, e 1509 ESTs (25%) não possuem qualquer similaridade a diferentes bancos de dados. Dentre as sequências anotadas, identificamos algumas sequências com alta similaridade a genes conhecidos em diferentes organismos, indicando a descoberta de alguns genes homólogos possivelmente envolvidos com processos carcinogênicos. Como exemplo, isolamos e caracterizamos parcialmente (i) uma nova isoforma do gene NABC1 (novel amplified sequence in breast carcinoma 1), o qual é pouco expresso em tumores coloretais, (ii) um novo gene da família de semaforinas (moléculas de motilidade axonal) que apresenta uma baixa expressão em linhagens celulares de glioblastoma tratadas com ácido retinóico, um agente antitumoral e (iii) o gene ortólogo humano Notch 2, aparentemente superexpresso em tumores mamários com maior malignidade. / Expressed sequence tags (ESTs) are of fundamental importance for the identification of genes within the human genome and defining gene expression characteristics. In this work, we describe a new approach for generating cDNA libraries using essentially arbitrary primers to construct PCR-based minilibraries from breast tumor mRNA. Clones from these libraries were sequenced to generate 6,029 ESTs. Using this approach, we were able to observe a significant normalization of the different mRNA subpopulations and a preferential amplification of the central portions of the genes. Bioinformatic analysis of these sequences shows that 3,350 ESTs (56%) have significant similarity to known DNA and/or cDNA sequences (annotated sequences) from different organisms and 1,509 ESTs (25%) show no similarity to any sequences on different databases. From the annotated sequences, we have identified some sequences with high similarity to known genes from different organisms, indicating the discovery of some homologous genes possibly correlated with carcinogenic processes. For instance, we have isolated and partially characterized (i) a new NABC1 (novel amplified sequence in breast carcinoma 1) isoform which is downregulated in colorectal tumors, (ii) a novel semaphorin member of axon guidance molecules that is down-regulated in glioblastoma cell lines treated with all-trans-retinoic acid, an anti-tumor agent and (iii) the ortolog Notch 2 human gene, apparenty overexpressed in breast tumors with higher malignancy.

Le séquençage du génome humain, il y a maintenant 12 ans, a mis en lumière la complexité des mécanismes des processus nucléaires tels que la transcription, la réplication ou l'organisation de la chromatine. Depuis, afin de mieux comprendre ces processus, un ensemble sans cesse croissant de données sur le noyau cellulaire a été produit et mis en ligne par un nombre important de laboratoires de par le monde. Ces données sont à la fois d'une richesse extraordinaire et d'une complexité embarrassante. Dans cette thèse, nous mettons à profit l'ensemble de ces données afin de mieux comprendre les déterminants nucléaires du programme spatio-temporel de réplication. Pour cela nous utilisons pas moins d'une centaine de profils épigénétiques ChiP-seq le long des chromosomes humains et dans diverses lignées cellulaires pour caractériser la structure primaire de la chromatine. Nous démontrons, à l'aide d'outils issus des statistiques multivariées, que l'immense complexité potentielle de ces jeux de données peut être réduite à quatre états chromatiniens principaux et ce dans toutes les lignées cellulaires somatiques étudiées. Cette classification simple, robuste et néanmoins complète est un excellent point d'appui pour l'étude de la réplication. Les quatre états principaux de chromatine sont répliqués à des moments distinct de la phase S (leur « timing » de réplication est différent) et ont un contenu en gènes drastiquement différents. Leur répartition spatiale le long du génome est structurée et est particulièrement visible dans les domaines où le « timing » de réplication dessine un U comme signature de l'existence d'un gradient de polarité des fourches de réplication. Ces U-domaines de la taille du Mpb recouvrent 50% du génome humain et les quatre états chromatiniens principaux se succèdent du bord au centre de ces U-domaines. Les mêmes techniques statistiques appliquées au cas d'une lignée embryonnaire révèlent aussi l'existence de quatre états principaux de chromatine mais de nature différente. La classification en quatre états s'avèrent alors très utile pour comparer l'épigénétique d'une lignée somatique à celle d'une lignée embryonnaire. Aussi, les spécificités du programme de réplication embryonnaire sont mises en rapport avec les spécificités de l'organisation de la chromatine dans cette lignée cellulaire. En particulier, notre étude révèle le rôle majeur de l'histone variant H2AZ dans la pluripotence. / The sequencing of the human genome, twelve years ago, revealed the complexity of the mechanisms underlying nuclear process such as transcription, replication and chromatin organization. In the past few years, to delineate better these processes, datasets on the cell nucleus were gathered and made available online by numerous laboratories around the world. These datasets are, at once, extraordinarily rich and daunting to handle. In this thesis, we take advantage of these datasets to understand better the nuclear determinants of the replication program. We analyze not less than a hundred ChiP-seq profiles along human chromosomes in several cell lines to characterize the primary structure of chromatin. We demonstrate, when using tools from multivariate statistics, that the immense potential complexity of these datasets can be reduced to four prevalent chromatin states in all studied somatic cell lines. This simple and comprehensive classification is an excellent starting point for the study of replication. The four prevalent chromatin states are replicated at different moments of the S-phase (they have a different replication “timing”) and have drasticaly different gene contents. Their spatial repartition along the genome is structured, especially in domains where the timing replication is U-shaped. These megabase sized U-domains cover 50% of the human genome and the four prevalent chromatin states succeed each other from their borders to their center. The same statistical techniques applied on an embryonic stem cell (ESC) also reduced the epigenetic complexity to four prevalent chromatin states which are qualitatively different from the ones in somatic cells. We further show that the specificities of embryonic replication program are link to the specificities of embryonic chromatin. Importantly, our study reveals that the histone variant H2AZ plays a major role in pluripotency.

As sequencing technology continues to produce better quality genomes at decreasing costs, there has been a recent surge in the variety of data that we are now able to analyze. This is particularly true with regards to our understanding of the human genome—where the last decade has seen data advances in primate epigenomics, ancient hominid genomics, and a proliferation of human polymorphism data from multiple populations. In order to utilize such data however, it has become critical to develop increasingly sophisticated tools spanning both bioinformatics and statistical inference. In population genetics particularly, new statistical approaches for analyzing population data are constantly being developed—unfortunately, often without proper model testing and evaluation of type-I and type-II error. Because the common Wright-Fisher assumptions underlying such models are generally violated in natural populations, this statistical testing is critical. Thus, my dissertation has two distinct but related themes: 1) evaluating methods of statistical inference in population genetics, and 2) utilizing these methods to analyze the evolutionary history of humans and our closest relatives. The resulting collection of work has not only provided important biological insights (including some of the first strong evidence of selection on human-specific epigenetic modifications (Shulha, Crisci, Reshetov, Tushir et al. 2012, PLoS Bio), and a characterization of human-specific genetic changes distinguishing modern humans from Neanderthals (Crisci et al. 2011, GBE)), but also important insights in to the performance of population genetic methodologies which will motivate the future development of improved approaches for statistical inference (Crisci et al, in review).

Indiana University-Purdue University Indianapolis (IUPUI) / The human genome encodes 19 members of the aldehyde dehydrogenase (ALDH) superfamily, critical enzymes involved in the metabolism of aldehyde substrates. A major function of the ALDH1A subfamily is the oxidation of retinaldehyde to retinoic acid, a key regulator of numerous cell growth and differentiation pathways. ALDH1A1 has been identified as a biomarker for both normal stem cells and cancer stem cells. Small molecule probes are needed to better understand the role of this enzyme in both normal and disease states. However, there are no commercially available, small molecules that selectively inhibit ALDH1A1. Our goal is to identify and characterize small molecule inhibitors of ALDH1A1 as chemical tools and as potential therapeutics. To better understand the basis for selective inhibition of ALDH1A1, we characterized N,N-diethylaminobenzaldehyde (DEAB), which is a commonly used inhibitor of ALDH1A1 and purported to be selective. DEAB serves as the negative control for the Aldefluor assay widely utilized to identify stem cells. Rather than being a selective inhibitor for ALDH1A1, we found that DEAB is a slow substrate for multiple ALDH isoenzymes, and depending on the rate of turnover, DEAB behaves as either a traditional substrate or as an inhibitor. Due to its very slow turnover, DEAB is a potent inhibitor of ALDH1A1 with respect to propionaldehyde oxidation, but it is not a good candidate for the development of selective ALDH1A1 inhibitors because of its promiscuity. Next, to discover novel selective inhibitors, we used an in vitro, high-throughput screen of 64,000 compounds to identify 256 hits that either activate or inhibit ALDH1A1 activity. We have characterized two structural classes of compounds, CM026 and CM037, using enzyme kinetics and X-ray crystallographic structural data. Both classes contained potent and selective inhibitors for ALDH1A1. Structural studies of ALDH1A1 with CM026 showed that CM026 binds at the active site, and its selectivity is achieved by a single residue substitution. Importantly, CM037 selectively inhibits proliferation of ALDH+ ovarian cancer cells. The discovery of these two selective classes of ALDH1A1 inhibitors may be useful in delineating the role of ALDH1A1 in biological processes and may seed the development of new chemotherapeutic agents.

[ES] En las últimas décadas, los avances en la tecnología de secuenciación han producido cantidades significativas de datos genómicos, hecho que ha revolucionado nuestra comprensión de la biología. Sin embargo, la cantidad de datos generados ha superado con creces nuestra capacidad para interpretarlos. Descifrar el código de la vida es un gran reto. A pesar de los numerosos avances realizados, nuestra comprensión del mismo sigue siendo mínima, y apenas estamos empezando a descubrir todo su potencial, por ejemplo, en áreas como la medicina de precisión o la farmacogenómica. El objetivo principal de esta tesis es avanzar en nuestra comprensión de la vida proponiendo una aproximación holística mediante un enfoque basado en modelos que consta de tres artefactos: i) un esquema conceptual del genoma, ii) un método para su aplicación en el mundo real, y iii) el uso de ontologías fundacionales para representar el conocimiento del dominio de una forma más precisa y explícita. Las dos primeras contribuciones se han validado mediante la implementación de sistemas de información genómicos basados en modelos conceptuales. La tercera contribución se ha validado mediante experimentos empíricos que han evaluado si el uso de ontologías fundacionales conduce a una mejor comprensión del dominio genómico. Los artefactos generados ofrecen importantes beneficios. En primer lugar, se han generado procesos de gestión de datos más eficientes, lo que ha permitido mejorar los procesos de extracción de conocimientos. En segundo lugar, se ha logrado una mejor comprensión y comunicación del dominio. / [CA] En les últimes dècades, els avanços en la tecnologia de seqüenciació han produït quantitats significatives de dades genòmiques, fet que ha revolucionat la nostra comprensió de la biologia. No obstant això, la quantitat de dades generades ha superat amb escreix la nostra capacitat per a interpretar-los. Desxifrar el codi de la vida és un gran repte. Malgrat els nombrosos avanços realitzats, la nostra comprensió del mateix continua sent mínima, i a penes estem començant a descobrir tot el seu potencial, per exemple, en àrees com la medicina de precisió o la farmacogenómica. L'objectiu principal d'aquesta tesi és avançar en la nostra comprensió de la vida proposant una aproximació holística mitjançant un enfocament basat en models que consta de tres artefactes: i) un esquema conceptual del genoma, ii) un mètode per a la seua aplicació en el món real, i iii) l'ús d'ontologies fundacionals per a representar el coneixement del domini d'una forma més precisa i explícita. Les dues primeres contribucions s'han validat mitjançant la implementació de sistemes d'informació genòmics basats en models conceptuals. La tercera contribució s'ha validat mitjançant experiments empírics que han avaluat si l'ús d'ontologies fundacionals condueix a una millor comprensió del domini genòmic. Els artefactes generats ofereixen importants beneficis. En primer lloc, s'han generat processos de gestió de dades més eficients, la qual cosa ha permés millorar els processos d'extracció de coneixements. En segon lloc, s'ha aconseguit una millor comprensió i comunicació del domini. / [EN] Over the last few decades, advances in sequencing technology have produced significant amounts of genomic data, which has revolutionised our understanding of biology. However, the amount of data generated has far exceeded our ability to interpret it. Deciphering the code of life is a grand challenge. Despite our progress, our understanding of it remains minimal, and we are just beginning to uncover its full potential, for instance, in areas such as precision medicine or pharmacogenomics. The main objective of this thesis is to advance our understanding of life by proposing a holistic approach, using a model-based approach, consisting of three artifacts: i) a conceptual schema of the genome, ii) a method for its application in the real-world, and iii) the use of foundational ontologies to represent domain knowledge in a more unambiguous and explicit way. The first two contributions have been validated by implementing genome information systems based on conceptual models. The third contribution has been validated by empirical experiments assessing whether using foundational ontologies leads to a better understanding of the genomic domain. The artifacts generated offer significant benefits. First, more efficient data management processes were produced, leading to better knowledge extraction processes. Second, a better understanding and communication of the domain was achieved. / Las fructíferas discusiones y los resultados derivados de los proyectos INNEST2021 /57, MICIN/AEI/10.13039/501100011033, PID2021-123824OB-I00, CIPROM/2021/023 y PDC2021- 121243-I00 han contribuido en gran medida a la calidad final de este tesis. / García Simón, A. (2022). Understanding the Code of Life: Holistic Conceptual Modeling of the Genome [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/191432

Des del 2003, any en què es va fer públic el desxiframent del Genoma Humà, hi ha hagut una allau de descobriments i aplicacions relacionats amb aquesta gran fita històrica i científica. Paral·lelament han anat sorgint diferents qüestionaments ètics; entre ells, aquells que es plantegen de quina manera es pot veure afectada la imatge de l'home en el desplegament de les possibilitats que ofereix aquesta singular troballa. En aquesta recerca s'estudien les diferents repercussions des d'un angle concret: el pensament de Hans Jonas. La proposta ètica desenvolupada per Hans Jonas permet fer una valoració acurada de la problemàtica que es dóna en alguns camps de la bioètica i, d'una manera especial, la que va associada amb la manipulació genètica. En aquesta tesi s'ofereix una sistematització dels plantejaments d'aquest autor per tal de facilitar la seva aplicació a situacions concretes.L'estudi del genoma i les pràctiques científiques, mèdiques i socials que se'n deriven, especialment les del genoma humà, han estat i constituiran en el futur una gran contribució per a la humanitat. Tanmateix, s'imposa la prudència en l'actuació de diferents estaments per preservar el bé de les persones, especialment d'aquelles més vulnerables.Un aspecte fonamental que es pot veure afectat per la tecnologia genètica aplicada a l'ésser humà és el mateix concepte d'home en la seva peculiaritat tridimensional: biològica, cultural i espiritual. En aquesta recerca es delimita l'especificitat humana en les seves diverses esferes tot oferint una orientació sobre l'eticitat de les intervencions genètiques per tal de preservar la dignitat de la persona humana.Es constata en aquest estudi que ciència i humanitats no són mons separats i que es fa necessari el diàleg constant entre les diverses disciplines perquè no és possible parlar de bioètica sense un clar i honest diàleg interdisciplinar. Les conclusions principals conflueixen en una qüestió que planteja una de les preocupacions principals de Jonas i també de l'autora: podrà la genètica arribar, amb el seu poder, a afectar allò més íntim que té la persona com és la seva essència, allò que li fa ser el que és? / Desde el 2003, año en que se hizo pública la descodificación del Genoma Humano, ha tenido lugar una ingente cantidad de descubrimientos y aplicaciones relacionados con este gran hito histórico y científico. Paralelamente han ido surgiendo diferentes cuestionamientos éticos; entre ellos, aquellos que plantean en qué medida puede verse afectada la imagen del hombre cuando se desplieguen las diversas posibilidades que ofrece el singular hallazgo. En investigación presente se estudian las diferentes repercusiones desde un ángulo concreto: el pensamiento de Hans Jonas. La propuesta ética desarrollada por Hans Jonas permite hacer una buena valoración de la problemática que se da en algunos campos de la bioética y, de una manera especial, la que va asociada con la manipulación genética. En esta tesis se ofrece una sistematización de los planteamientos del autor, con la pretensión de facilitar su aplicación a situaciones concretas. El estudio del genoma y las prácticas científicas, médicas y sociales que se desprenden de él, especialmente las que afectan al genoma humano, han sido y constituirán en el futuro una gran contribución para la humanidad. Aun así, se impone la prudencia en la actuación de diferentes estamentos si se quiere preservar el bien de las personas, especialmente el de las más vulnerables. Un aspecto fundamental que puede verse afectado por la tecnología genética aplicada al ser humano es el mismo concepto de hombre en su peculiaridad tridimensional: biológica, cultural y espiritual. En esta tesis se delimita la especificidad humana en sus diversas esferas ofreciendo diversas orientaciones sobre la eticidad de las intervenciones genéticas de manera que se pueda preservar la dignidad de la persona humana. Se constata en este estudio que ciencia y humanidades no son mundos separados y que se hace necesario el diálogo constante entre las diversas disciplinas porque no es posible hablar de bioética sin un claro y honesto diálogo interdisciplinar. Las conclusiones principales confluyen en una cuestión que plantea una de las preocupaciones principales de Jonas y también de la autora: ¿podrá la genética llegar, con su poder, a afectar lo más íntimo que tiene la persona como es su esencia, aquello que le hace ser lo que es? / The human genome was first completed in 2003 and since then there has been a surge of discoveries and applications related to that historic and scientific deed. Different ethical issues have arisen in parallel posing the question of how man's image can be affected by the immense quantity of ramifications that the finding itself possesses. In this study we have focused on the impact of the human genome's applications from the perspective of Hans Jonas' thinking. His ethical approach will allow us to assess accurately the problems associated with genetic manipulation which occur in some fields of bioethics. This study aims to systematize points of view so as to make a future practical application less complex. The study of the human genome and its scientific, medical and social practices have been and will continue to be a great contribution to mankind. Nonetheless, caution and good sense should prevail when applying its results in order to protect those members of society who are more vulnerable.The concept of human being in its biological, cultural and spiritual could be negatively affected by genetic technology so this research highlights human uniqueness within different spheres while offering ethical guidance on how to preserve human dignity in genetic interventions.This study states both that science and humanities are not two worlds apart and that an ongoing dialogue within different disciplines is very much needed. It makes no sense to talk about bioethics without opening first an honest interdisciplinary dialogueThe main conclusions of this study point at an issue which is both mine and Jona's top priorities: Will genetic engineering eventually have a negative impact on the essence of human's core values?

It has been suggested that organismal complexity correlates with the complexity of gene regulation. Transcriptional control of gene expression is mediated by binding of regulatory proteins to cis-acting sequences on the genome. Hence, it is crucial to identify the chromosomal targets of transcription factors (TFs) to delineate transcriptional regulatory networks underlying gene expression programs. The development of ChIP-chip technology has enabled high throughput mapping of TF binding sites across the genome. However, there are many limitations to the technology including the availability of whole genome arrays for complex organisms such human or mouse. To circumvent these limitations, we developed the Sequence Tag Analysis of Genomic Enrichment (STAGE) methodology that is based on extracting short DNA sequences or “tags” from ChIP-enriched DNA. With improvements in sequencing technologies, we applied the recently developed ChIP-Seq technique i.e. ChIP followed by ultra high throughput sequencing, to identify binding sites for the TF E2F4 across the human genome. We identified previously uncharacterized E2F4 binding sites in intergenic regions and found that several microRNAs are potential E2F4 targets. Binding of TFs to their respective chromosomal targets requires access of the TF to its regulatory element, which is strongly influenced by nucleosomal remodeling. In order to understand nucleosome remodeling in response to transcriptional perturbation, we used ultra high throughput sequencing to map nucleosome positions in yeast that were subjected to heat shock or were grown normally. We generated nucleosome remodeling profiles across yeast promoters and found that specific remodeling patterns correlate with specific TFs active during the transcriptional reprogramming. Another important aspect of gene regulation operates at the post-transcriptional level. MicroRNAs (miRNAs) are ~22 nucleotide non-coding RNAs that suppress translation or mark mRNAs for degradation. MiRNAs regulate TFs and in turn can be regulated by TFs. We characterized a TF-miRNA network involving the oncofactor Myc and the miRNA miR-22 that suppresses the interferon pathway as primary fibroblasts enter a stage of rapid proliferation. We found that miR-22 suppresses the interferon pathway by inhibiting nuclear translocation of the TF NF-kappaB. Our results show how the oncogenic TF Myc cross-talks with other TF regulatory pathways via a miRNA intermediary. / text

Thesis (Ph. D.)--Ohio State University, 2007. / Full text release at OhioLINK's ETD Center delayed at author's request