CUNHA, Aline Gonzaga. Atividade de enzimas da via de síntese dos fenólicos durante o desenvolvimento de pedúnculos de clones de cajueiro (Anacardium occidentale L.). 2015. 60 f. Dissertação (Mestrado em Bioquímica)-Universidade Federal do Ceará, Fortaleza, 2015. / Submitted by Eric Santiago (erichhcl@gmail.com) on 2016-08-24T14:29:59Z No. of bitstreams: 1 2015_dis_agcunha.pdf: 1915595 bytes, checksum: ddc88d25dd660aa93b503d82263a5624 (MD5) / Approved for entry into archive by Jairo Viana (jairo@ufc.br) on 2016-08-26T18:37:01Z (GMT) No. of bitstreams: 1 2015_dis_agcunha.pdf: 1915595 bytes, checksum: ddc88d25dd660aa93b503d82263a5624 (MD5) / Made available in DSpace on 2016-08-26T18:37:01Z (GMT). No. of bitstreams: 1 2015_dis_agcunha.pdf: 1915595 bytes, checksum: ddc88d25dd660aa93b503d82263a5624 (MD5) Previous issue date: 2015 / The dwarf cashew apple (Anacardium occidentale L.) is a tropical plant, originated in Brazil, which is fruit species with high potential for fresh consumption and for industrial processing. With the discovery of bioactive compounds in the cashew apple are necessary studies of the activity of enzymes of the synthesis of phenolic compounds, such as phenylalanine ammonia lyase (PAL), UDP-glucose: trans-cinnamate β-D-glycosyltransferase (UGT), flavonol synthase (FLS) and leucoanthocyanidin reductase (LAR). Soon, this study aimed to determine the activity of these enzymes in CCP 76 and BRS 189 dwarf cashew clones in three different stages of maturation, and to establish the phenolic profile in these stages. Reaction products were identified and quantified by LC-DAD. PAL activity decreases with maturation for dwarf cashew clones. UGT enzyme in the CCP 76 clone stage 4 showed the highest enzyme specific activity than in other stages of development for that clone. Whereas BRS 189 clone, the highest activity was in the mature cashew apple. Quercetin synthesis was evidenced in stage 4 for the two clones analyzed. The enzymes involved in the metabolism phenolic behave differently, showing the complexity of this biosynthetic pathway that varies between clones and during the development of cashew apple. Further studies, with mass spectrometer linked HPLC, will elucidate the profile of the compounds present in cashews apple lyophilized for chemical characterization of these in different stages of maturation. Therefore, the understanding of the enzymatic metabolism between the clones and during cashew apple development represent a real potential for the development of new products with functional properties and promotion of human health. / O cajueiro (Anacardium occidentale L.) é uma planta tropical, originária do Brasil, que se destaca como espécie frutífera com elevada potencialidade para o consumo in natura e para o processamento industrial. Com a descoberta de compostos bioativos presentes nos pedúnculos, se fazem necessários estudos da atividade de enzimas da síntese dos compostos fenólicos, tais como a fenilalanina amônia liase (PAL), UDP-glicose: trans-cinamato β-D- glicosiltransferase (UGT), flavonol sintase (FLS) e leucoantocianidina redutase (LAR). Logo, esse estudo objetivou determinar a atividade dessas enzimas, em pseudofrutos de cajueiro-anão-precoce dos clones CCP 76 e BRS 189 em três diferentes estádios de maturação, além de estabelecer o perfil de fenólicos nestes referidos estádios. Os produtos resultantes das reações enzimáticas foram identificados e quantificados por LC-DAD. A atividade da PAL decresce com o amadurecimento dos pseudofrutos. A enzima UGT no estádio 4 do clone CCP 76 apresentou a maior atividade enzimática específica que nos outros estádios de desenvolvimento para o referido clone. Enquanto no clone BRS 189, a maior atividade deteve-se no caju maduro. A maior síntese de quercetina no caju foi evidenciada no estádio 4 para os dois clones estudados. As enzimas envolvidas no metabolismo de fenólicos se comportam diferentemente, demonstrando a complexidade dessa rota biossintética que varia entre os clones e durante o desenvolvimento dos pedúnculos de cajueiro. Estudos posteriores, com CLAE acoplada a espectrômetro de massa, irão elucidar melhor o perfil dos compostos presentes em cajus liofilizados para caraterização química destes em diferentes estádios de maturação. Portanto, o entendimento do metabolismo enzimático entre os clones e durante o desenvolvimento do pedúnculo representam um potencial real para o desenvolvimento de novos produtos com propriedades funcionais e promoção da saúde humana.

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / The dwarf cashew apple (Anacardium occidentale L.) is a tropical plant, originated in Brazil, which is fruit species with high potential for fresh consumption and for industrial processing. With the discovery of bioactive compounds in the cashew apple are necessary studies of the activity of enzymes of the synthesis of phenolic compounds, such as phenylalanine ammonia lyase (PAL), UDP-glucose: trans-cinnamate β-D-glycosyltransferase (UGT), flavonol synthase (FLS) and leucoanthocyanidin reductase (LAR). Soon, this study aimed to determine the activity of these enzymes in CCP 76 and BRS 189 dwarf cashew clones in three different stages of maturation, and to establish the phenolic profile in these stages. Reaction products were identified and quantified by LC-DAD. PAL activity decreases with maturation for dwarf cashew clones. UGT enzyme in the CCP 76 clone stage 4 showed the highest enzyme specific activity than in other stages of development for that clone. Whereas BRS 189 clone, the highest activity was in the mature cashew apple. Quercetin synthesis was evidenced in stage 4 for the two clones analyzed. The enzymes involved in the metabolism phenolic behave differently, showing the complexity of this biosynthetic pathway that varies between clones and during the development of cashew apple. Further studies, with mass spectrometer linked HPLC, will elucidate the profile of the compounds present in cashews apple lyophilized for chemical characterization of these in different stages of maturation. Therefore, the understanding of the enzymatic metabolism between the clones and during cashew apple development represent a real potential for the development of new products with functional properties and promotion of human health. / O cajueiro (Anacardium occidentale L.) Ã uma planta tropical, originÃria do Brasil, que se destaca como espÃcie frutÃfera com elevada potencialidade para o consumo in natura e para o processamento industrial. Com a descoberta de compostos bioativos presentes nos pedÃnculos, se fazem necessÃrios estudos da atividade de enzimas da sÃntese dos compostos fenÃlicos, tais como a fenilalanina amÃnia liase (PAL), UDP-glicose: trans-cinamato β-D- glicosiltransferase (UGT), flavonol sintase (FLS) e leucoantocianidina redutase (LAR). Logo, esse estudo objetivou determinar a atividade dessas enzimas, em pseudofrutos de cajueiro-anÃo-precoce dos clones CCP 76 e BRS 189 em trÃs diferentes estÃdios de maturaÃÃo, alÃm de estabelecer o perfil de fenÃlicos nestes referidos estÃdios. Os produtos resultantes das reaÃÃes enzimÃticas foram identificados e quantificados por LC-DAD. A atividade da PAL decresce com o amadurecimento dos pseudofrutos. A enzima UGT no estÃdio 4 do clone CCP 76 apresentou a maior atividade enzimÃtica especÃfica que nos outros estÃdios de desenvolvimento para o referido clone. Enquanto no clone BRS 189, a maior atividade deteve-se no caju maduro. A maior sÃntese de quercetina no caju foi evidenciada no estÃdio 4 para os dois clones estudados. As enzimas envolvidas no metabolismo de fenÃlicos se comportam diferentemente, demonstrando a complexidade dessa rota biossintÃtica que varia entre os clones e durante o desenvolvimento dos pedÃnculos de cajueiro. Estudos posteriores, com CLAE acoplada a espectrÃmetro de massa, irÃo elucidar melhor o perfil dos compostos presentes em cajus liofilizados para caraterizaÃÃo quÃmica destes em diferentes estÃdios de maturaÃÃo. Portanto, o entendimento do metabolismo enzimÃtico entre os clones e durante o desenvolvimento do pedÃnculo representam um potencial real para o desenvolvimento de novos produtos com propriedades funcionais e promoÃÃo da saÃde humana.

Generating a video signal for ATtiny85 , authoring software worldwide for video game console ATARI2600 on the theme of UAV attacks and their media image - a reinterpretation of using "low res" generated video.

The purpose of my study is to analyze Assyrian sculptures in the palace of Nimrud from throne room of Aššur-nāṣir-apli northwest palace. My research will be to compare sculptures with a theoretical as well as practical issue in the design of the galleries. Images of supernatural beings would be set up at entrances to palaces and temples. Assyrians called sculptures Lamassū (from written Sumerian references LAM(M) (A) lord of horizons, guardian of the Assyrian Gate. Lamassū are both flanked at the doorways from the throne room of Aššur-nāṣir-apli`s northwest palace. The exact meaning is not clear, but Lamassū can be taken as representing an Assyrian protective divinity. The first method in my analysis combines the need to establish the subjective meaning of objects as objective reality that is their meaning for digital design. My thesis aims to define a specific iconographic theme, centered around sculptures based on similarities in the composition of each scene in the selection of images on monuments in Nimrud. Digitalization projects provide an angle from mergers areas as architecture and archeology through images. With the project historians can use traditional documents and images to reconstruct the past and palaces. The analysis takes as an example of the importance of Digital archeology in understanding the role of artefacts and the role as a function of the specific purpose or activities for which such present or used in museums. We construct meaning as the basis for action, and not only from concrete material, but also from the matrix of symbols that are available from within culture to interpret the substantive conditions. Digital archeology is associated with technology that provides a picture of the physical environment. The digital images show the limit between design of the architectural spaces from the British Museum and the old spaces in Nimrud.

Los residuos lignocelulósicos son los sub-productos agroindustriales de mayor abundancia en el sector agrícola, por esta razón, se ha propuesto el uso de estos en diferentes aplicaciones de materiales compuestos. El objetivo de este trabajo es explorar el potencial del uso de la hoja caulinar del bambú (HCB) y el peciolo envainador de la hoja de la palmera (PHP), para la producción de laminados decorativos, con el fin de ser usados en revestimientos de interiores de edificios. Se realizó primero la caracterización de la HCB y el PHP en estado natural, luego se realizó un prototipo de fabricación del revestimiento y por último, la caracterización del laminado que se obtuvo en este proceso. Los resultados muestran que el comportamiento termodinámico de la HCB y el PHP, en el proceso de laminación no debe superar los ~135°. El análisis físico-químico muestra que la porosidad y la presencia de minerales son mayores en las HCB en relación a la PHP. En el envejecimiento acelerado por CUV, se determinó que las HCB son más susceptibles al cambio de color en su superficie. En cuanto al proceso de laminación, las HCB necesariamente deben pasar por el proceso de rectificado antes de la laminación. En el proceso de laminación, se determinó que el uso del substrato de papel kraft impregnado es el más conveniente para la conservación de la estética de la superficie de ambos residuos. De acuerdo con la topografía de la superficie, podemos afirmar que los patrones de rugosidad disminuyen con respecto al proceso de laminación, en la HCB pasan de 0,017mm para 0,008mm y de 0,015mm para 0,027mm en la superficie externa e interna respectivamente; en el PHP pasan de 0,076 para 0,016 y de 0,106mm para 0,029mm. Aunque en la HCB, se observe el perfil (y) un aumento de la ondulación. Después del proceso de laminación la porosidad disminuye en ambos residuos y los valores de absorción de agua disminuyen 7,25% en la HCB y aumentan 1,33% en el PHP. Podemos concluir que ambas superficies son aptas para uso en revestimientos. / Lignocellulosic residues are the most abundant agri-bussiness product in the agricultural sector, therefore, it has been proposed for use in various applications of these composites. The aim of this paper is to explore the potential of using sheath bamboo leaf (SBL) and the petiole leaf palm (PLP), for the production of decorative laminates, in order to be used in coatings inside buildings. Characterization of SBL and PLP unprocessed was performed, then a prototype process manufacturing and finally characterization laminate coating obtained was performed. The results show that the thermodynamic behavior of SBL and PLP in the lamination process should not exceed ~ 135 °C. The physico-chemical analysis shows that the porosity and the presence of mineral on SBL are greater than PHP. In the accelerated aging by CUV method, it was determined that SBL are suseptible to color change on the surface. The SBL necessarily have to go through the process of grinding prior to lamination. In the lamination process, it was determined that the use of kraft paper impregnated substrate is most suitable for preserving the aesthetics of the surface of both residues. According to the topography of the surface, we can say that the patterns of roughness decrease with respect to the lamination process, on SBL change from 0,017mm to 0,008mm and 0,015mm to 0,027mm in the outer and inner surface, respectively; PLP roughness change from 0.076 to 0.016 and 0,106mm to 0,029mm. Although the profile (y) increased ripple is observed at the SLB. After the lamination process the porosity decreases in both waste and water absorption values decrease on SLB 7.25% and 1.33% increase in the PLP. We concluded that both surfaces are suitable for use in coatings.

Made available in DSpace on 2015-04-14T13:47:35Z (GMT). No. of bitstreams: 1 433597.pdf: 15467920 bytes, checksum: 9f51c8872afcde7d0ee58dd5e8cbe752 (MD5) Previous issue date: 2011-08-26 / A presente disserta??o tem por objetivo central analisar os murais elaborados por Aldo Locatelli, entre os anos de 1951 e 1955, nas salas e sal?es do segundo piso da Ala Governamental do Pal?cio Piratini. Ao serem trabalhados as pinturas A forma??o hist?rico-etnogr?fica do povo rio-grandense, A funda??o de Rio Grande (ambos elaborados no Sal?o Alberto Pasqualini), O Negrinho do Pastoreio (localizado no Sal?o que leva o mesmo nome) e O Estado (executado na Antessala do Gabinete do Governador), pretende-se, fundamentalmente, estabelecer a rede de rela??es que envolveram tanto o artista quanto os elementos externos ? sua produ??o pict?rica. Nesse sentido, a partir do estudo dos murais de car?ter hist?rico, formativo e folcl?rico que aludem ao Rio Grande do Sul, buscar-se-? analisar as representa??es e as quest?es formais presentes nas citadas obras, em conjunto com os elementos do contexto cultural e intelectual do per?odo.

Los residuos lignocelulósicos son los sub-productos agroindustriales de mayor abundancia en el sector agrícola, por esta razón, se ha propuesto el uso de estos en diferentes aplicaciones de materiales compuestos. El objetivo de este trabajo es explorar el potencial del uso de la hoja caulinar del bambú (HCB) y el peciolo envainador de la hoja de la palmera (PHP), para la producción de laminados decorativos, con el fin de ser usados en revestimientos de interiores de edificios. Se realizó primero la caracterización de la HCB y el PHP en estado natural, luego se realizó un prototipo de fabricación del revestimiento y por último, la caracterización del laminado que se obtuvo en este proceso. Los resultados muestran que el comportamiento termodinámico de la HCB y el PHP, en el proceso de laminación no debe superar los ~135°. El análisis físico-químico muestra que la porosidad y la presencia de minerales son mayores en las HCB en relación a la PHP. En el envejecimiento acelerado por CUV, se determinó que las HCB son más susceptibles al cambio de color en su superficie. En cuanto al proceso de laminación, las HCB necesariamente deben pasar por el proceso de rectificado antes de la laminación. En el proceso de laminación, se determinó que el uso del substrato de papel kraft impregnado es el más conveniente para la conservación de la estética de la superficie de ambos residuos. De acuerdo con la topografía de la superficie, podemos afirmar que los patrones de rugosidad disminuyen con respecto al proceso de laminación, en la HCB pasan de 0,017mm para 0,008mm y de 0,015mm para 0,027mm en la superficie externa e interna respectivamente; en el PHP pasan de 0,076 para 0,016 y de 0,106mm para 0,029mm. Aunque en la HCB, se observe el perfil (y) un aumento de la ondulación. Después del proceso de laminación la porosidad disminuye en ambos residuos y los valores de absorción de agua disminuyen 7,25% en la HCB y aumentan 1,33% en el PHP. Podemos concluir que ambas superficies son aptas para uso en revestimientos. / Lignocellulosic residues are the most abundant agri-bussiness product in the agricultural sector, therefore, it has been proposed for use in various applications of these composites. The aim of this paper is to explore the potential of using sheath bamboo leaf (SBL) and the petiole leaf palm (PLP), for the production of decorative laminates, in order to be used in coatings inside buildings. Characterization of SBL and PLP unprocessed was performed, then a prototype process manufacturing and finally characterization laminate coating obtained was performed. The results show that the thermodynamic behavior of SBL and PLP in the lamination process should not exceed ~ 135 °C. The physico-chemical analysis shows that the porosity and the presence of mineral on SBL are greater than PHP. In the accelerated aging by CUV method, it was determined that SBL are suseptible to color change on the surface. The SBL necessarily have to go through the process of grinding prior to lamination. In the lamination process, it was determined that the use of kraft paper impregnated substrate is most suitable for preserving the aesthetics of the surface of both residues. According to the topography of the surface, we can say that the patterns of roughness decrease with respect to the lamination process, on SBL change from 0,017mm to 0,008mm and 0,015mm to 0,027mm in the outer and inner surface, respectively; PLP roughness change from 0.076 to 0.016 and 0,106mm to 0,029mm. Although the profile (y) increased ripple is observed at the SLB. After the lamination process the porosity decreases in both waste and water absorption values decrease on SLB 7.25% and 1.33% increase in the PLP. We concluded that both surfaces are suitable for use in coatings.

Cette thèse se compose en deux parties: Première Partie: La conception et la synthèse d’analogues pyrrolidiniques, utilisés comme agents anticancéreux, dérivés du FTY720. FTY720 est actuellement commercialisé comme médicament (GilenyaTM) pour le traitement de la sclérose en plaques rémittente-récurrente. Il agit comme immunosuppresseur en raison de son effet sur les récepteurs de la sphingosine-1-phosphate. A fortes doses, FTY720 présente un effet antinéoplasique. Cependant, à de telles doses, un des effets secondaires observé est la bradycardie dû à l’activation des récepteurs S1P1 et S1P3. Ceci limite son potentiel d’utilisation lors de chimiothérapie. Nos précédentes études ont montré que des analogues pyrrolidiniques dérivés du FTY720 présentaient une activité anticancéreuse mais aucune sur les récepteurs S1P1 et S1P3. Nous avons soumis l’idée qu’une étude relation structure-activité (SARs) pourrait nous conduire à la découverte de nouveaux agents anti tumoraux. Ainsi, deux séries de composés pyrrolidiniques (O-arylmethyl substitué et C-arylmethyl substitué) ont pu être envisagés et synthétisés (Chapitre 1). Ces analogues ont montré d’excellentes activités cytotoxiques contre diverses cellules cancéreuses humaines (prostate, colon, sein, pancréas et leucémie), plus particulièrement les analogues actifs qui ne peuvent pas être phosphorylés par SphK, présentent un plus grand potentiel pour le traitement du cancer sans effet secondaire comme la bradycardie. Les études mécanistiques suggèrent que ces analogues de déclencheurs de régulation négative sur les transporteurs de nutriments induisent une crise bioénergétique en affamant les cellules cancéreuses. Afin d’approfondir nos connaissances sur les récepteurs cibles, nous avons conçu et synthétisé des sondes diazirine basées sur le marquage d’affinité aux photons (méthode PAL: Photo-Affinity Labeling) (Chapitre 2). En s’appuyant sur la méthode PAL, il est possible de récolter des informations sur les récepteurs cibles à travers l’analyse LC/MS/MS de la protéine. Ces tests sont en cours et les résultats sont prometteurs. Deuxième partie: Coordination métallique et catalyse di fonctionnelle de dérivés β-hydroxy cétones tertiaires. Les réactions de Barbier et de Grignard sont des méthodes classiques pour former des liaisons carbone-carbone, et généralement utilisées pour la préparation d’alcools secondaires et tertiaires. En vue d’améliorer la réaction de Grignard avec le 1-iodobutane dans les conditions « one-pot » de Barbier, nous avons obtenu comme produit majoritaire la β-hydroxy cétone provenant de l’auto aldolisation de la 5-hexen-2-one, plutôt que le produit attendu d’addition de l’alcool (Chapitre 3). La formation inattendue de la β-hydroxy cétone a également été observée en utilisant d’autres dérivés méthyl cétone. Étonnement dans la réaction intramoléculaire d’une tricétone, connue pour former la cétone Hajos-Parrish, le produit majoritaire est rarement la β-hydroxy cétone présentant la fonction alcool en position axiale. Intrigué par ces résultats et après l’étude systématique des conditions de réaction, nous avons développé deux nouvelles méthodes à travers la synthèse sélective et catalytique de β-hydroxy cétones spécifiques par cyclisation intramoléculaire avec des rendements élevés (Chapitre 4). La réaction peut être catalysée soit par une base adaptée et du bromure de lithium comme additif en passant par un état de transition coordonné au lithium, ou bien soit à l’aide d’un catalyseur TBD di fonctionnel, via un état de transition médiée par une coordination bidenté au TBD. Les mécanismes proposés ont été corroborés par calcul DFT. Ces réactions catalytiques ont également été appliquées à d’autres substrats comme les tricétones et les dicétones. Bien que les efforts préliminaires afin d’obtenir une enantioselectivité se sont révélés sans succès, la synthèse et la recherche de nouveaux catalyseurs chiraux sont en cours. / This thesis consists of two parts: Part 1: Design and synthesis of constrained azacyclic pyrrolidine analogues of FTY720 as anticancer agents FTY720 is presently marketed as a drug (GilenyaTM) for the treatment of relapsing-remitting multiple sclerosis. It functions as an immunosuppressant due to its effect on sphingosine-1-phosphate (S1P) receptors. At higher doses, FTY720 also has antineoplastic actions. However, at such doses it induces bradycardia due to the activation of the S1P1 and S1P3 receptors. This limits its potentical to be used as a cancer therapy in humans. Our previous studies have shown that some constrained pyrrolidine analogues of FTY720 have anticancer activity but no activity toward S1P1 and S1P3 receptors. We reasoned that a study of the structure-activity relationships (SARs) could lead to the discovery of new effective antitumor agents. Thus, two series of constrained analogues (O-arylmethyl-substituted pyrrolidines and C-aryl-substituted pyrrolidines) were designed and synthesized (Chapter 1). These analogues showed excellent cytotoxic activity against various human cancer cells (prostate, colon, breast, pancreas and leukemia). Especially, several active analogues, which cannot be phosphorylated by SphK, have the potency to be further studied in the treatment of cancer without inducing bradycardia. Mechanistic studies suggest that these constrained analogues trigger down-regulation of nutrient transporters, which induce a bioenergetic crisis and the cancer cells starve to death. To further investigate their target receptors, we have designed and synthesized diazirine based photo-affinity labeling (PAL) probes (Chapter 2). Aided by the PAL technique, information regarding the target receptor could be obtained through LC/MS/MS protein analysis. These tests are in progress and the preliminary results appear promising. Part 2: Metal coordination-controlled and bifunctional catalysis toward tertiary β-ketols The Barbier and Grignard reactions are classical methods to form carbon-carbon bonds, and generally used to prepare secondary or tertiary alcohols. In an attempt to perform a Grignard reaction with n-butyl iodide under Barbier one-pot conditions, we obtained major product β-hydroxyl ketol from the self-aldol reaction of 5-hexen-2-one, rather than the expected addition alcohol product (Chapter 3). The unusual β-ketol formation was also observed using other methyl ketone substrates. Interestingly, in an intramolecular reaction of a triketone substrate, which is well known to give the Hajos-Parrish ketone, the favored product was a rarely studied β-ketol with the hydroxyl group at axial position. Intrigued by these results, after systematic reaction condition studies, we developed two new methods toward the catalytic synthesis of specific β-ketols by intramolecular cylcization in high yield and selectivity (Chapter 4). The reaction can be catalyzed either by a suitable base and lithium bromide as the additive, through a lithium pre-organized transition state or by a bifunctional catalyst TBD (triazabicyclodecene), through a TBD mediated bidentate transition state. The proposed mechanisms were corroborated by DFT computation. These catalytic reactions were also extended to other triketone and diketone substrates. Although the initial efforts to achieve enantioselectivity were not successful, they merit further study of the synthesis and investigation of new chiral catalysts.

This diploma thesis treats the possibilities of the black-white picture depiction in pseudo colors (Pseudocolor). The individual methods, software or hardware are described there and the detailed block scheme of the system Pseudocolor is suggested. The block scheme is created on the basis of gained theoretical knowledge. In the thesis, individual functional blocks of the scheme are described and their circuit designs are realized. Some of the functional blocks are simulated by the PSpice program and accompanied by corresponding signal process data. The suitable choice of the active and passive components is performed, from which the general integration of the system Pseudocolor is made. All the source materials for the realization of the device are given there – double-sided drawing of the printed circuit, layout and specification of the components.

Esta dissertação realiza uma análise do livro A Máquina do Tempo, escrito por H.G. Wells em 1895, e sua transposição fílmica homônima realizada por George Pal em 1960 dentro do escopo do gênero ficção-científica. São analisadas as mudanças que foram realizadas no processo de transposição da literatura para o cinema. Este trabalho discute inicialmente as características do gênero ficção-científica na literatura, como o gênero se diferencia de outros gêneros como a literatura fantástica, por exemplo, e quais são os elementos que compõem a identidade do gênero ficção-científica. Em um segundo momento investigamos os problemas que o gênero ficção-científica apresenta quando transposto a mídia cinematográfica. Entre os fatores investigados estão como a verossimilhança funciona na literatura de ficção-científica e como o leitor se relaciona com ela. Também se analisou como o gênero utiliza o conhecimento científico para criar seus universos ficcionais, e as mudanças que os realizadores fílmicos às vezes precisam fazer para tornar o universo literário ficcional aceitável ao espectador em termos de verossimilhança, considerando que o que é verossímil na literatura não é necessariamente verossímil no cinema, especialmente na literatura de ficção-científica, um gênero que possui uma ligação íntima com a ciência e o progresso. Para finalizar essa pesquisa analisa a relação entre o gênero ficção-científica e o cinema no que diz respeito aos problemas que o gênero apresenta ao cinema em termos de tecnologia. Em outras palavras, o gênero literário ficção-científica traz aos realizadores fílmicos problemas e desafios que exigem a pesquisa e o desenvolvimento de novas tecnologias e técnicas visando resolvê-los. / The present work analyzes the book The Time Machine, written by H.G.Wells in 1895, as well as its transposition into a homonymous movie made by George Pal in 1960 as part of the science fiction genre. This work initially discusses the characteristics of the science fiction genre in literature, the way the genre differs from others such as the fantastic literature, for instance, and the fundamental elements of the science fiction genre. It then investigates the problems the science fiction genre presents when transposed to film media. Among the investigated factors are the problem of verisimilitude in science fiction literature and how the reader responds to it. This includes discussing the way the genre uses scientific knowledge to create its fictional universe, and the changes, in terms of verisimilitude, moviemakers need to consider when the fictional literary text is transferred into film. What sometimes is plausible in literature not always seems plausible in movies, especially in regard to science fiction literature, a genre that has a intimacy with science and progress. To conclude, this research analyzes the relation between the science fiction genre and film concerning the challenges the genre presents when transposed into another medium in terms of technology. These challenges require research and the development of new procedures for the cinematographic medium.