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Metody rozptylu světla a kalorimetrie ve studiu systémů hyaluronan-albumin / Study of hyaluronan-albumin systems using light scattering methods and calorimetrySereda, Alena January 2016 (has links)
This thesis, which is a continuation of the previous Bachelor thesis, is dedicated to the study of polyelectrolyte complexes between hyaluronan (HA) and bovine serum albumin (BSA). Interactions between HA and BSA and a formation of complexes were studied by SEC-MALLS method, where a molar mass, a root mean square (rms) radius, a hydronamic radius and an intrinsic viscosity of particles of the system were defined. Furthermore the interactions were studied by calorimetric measurement ITC, where thermodynamic character of complex formation was determined. Additionally, dynamic and electrophoretic light scattering methods (DLS and ELS) were applied, where hydrodynamic radius and a value of the zeta potential were defined. Also the effect of higher temperature on the character and complex formation was examined by SEC-MALLS and ITC measurements. The interactions were confirmed at any of the used media, but with different efficiency. It was also proved that complexes become smaller in their radii and viscosity with growing BSA concentration. Furthermore it was proved, that the high ionic strength hinders surface charges of HA and BSA molecules and minimizes their mutual interactions. At the higher ionic strength the radii of the complexes, their intrinsic viscosity and zeta potential are increased. The higher temperature has only minimal effect on the formation of the complexes.
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Optimisation of adaptive localisation techniques for cognitive radioThomas, Robin Rajan 06 August 2012 (has links)
Spectrum, environment and location awareness are key characteristics of cognitive radio (CR). Knowledge of a user’s location as well as the surrounding environment type may enhance various CR tasks, such as spectrum sensing, dynamic channel allocation and interference management. This dissertation deals with the optimisation of adaptive localisation techniques for CR. The first part entails the development and evaluation of an efficient bandwidth determination (BD) model, which is a key component of the cognitive positioning system. This bandwidth efficiency is achieved using the Cramer-Rao lower bound derivations for a single-input-multiple-output (SIMO) antenna scheme. The performances of the single-input-single-output (SISO) and SIMO BD models are compared using three different generalised environmental models, viz. rural, urban and suburban areas. In the case of all three scenarios, the results reveal a marked improvement in the bandwidth efficiency for a SIMO antenna positioning scheme, especially for the 1×3 urban case, where a 62% root mean square error (RMSE) improvement over the SISO system is observed. The second part of the dissertation involves the presentation of a multiband time-of arrival (TOA) positioning technique for CR. The RMSE positional accuracy is evaluated using a fixed and dynamic bandwidth availability model. In the case of the fixed bandwidth availability model, the multiband TOA positioning model is initially evaluated using the two-step maximum-likelihood (TSML) location estimation algorithm for a scenario where line-of-sight represents the dominant signal path. Thereafter, a more realistic dynamic bandwidth availability model has been proposed, which is based on data obtained from an ultra-high frequency spectrum occupancy measurement campaign. The RMSE performance is then verified using the non-linear least squares, linear least squares and TSML location estimation techniques, using five different bandwidths. The proposed multiband positioning model performs well in poor signal-to-noise ratio conditions (-10 dB to 0 dB) when compared to a single band TOA system. These results indicate the advantage of opportunistic TOA location estimation in a CR environment. / Dissertation (MEng)--University of Pretoria, 2012. / Electrical, Electronic and Computer Engineering / unrestricted
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A Fully Analog Motion Artifacts and Baseline Wander Elimination Circuit for Ambulatory ECG Recording SystemsNazari, Masoud 01 May 2023 (has links)
No description available.
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Macroscopic and Microscopic surface features of Hydrogenated silicon thin filmsPepenene, Refuoe Donald January 2018 (has links)
Magister Scientiae - MSc (Physics) / An increasing energy demand and growing environmental concerns regarding the use of fossil
fuels in South Africa has led to the challenge to explore cheap, alternative sources of energy.
The generation of electricity from Photovoltaic (PV) devices such as solar cells is currently
seen as a viable alternative source of clean energy. As such, crystalline, amorphous and
nanocrystalline silicon thin films are expected to play increasingly important roles as
economically viable materials for PV development. Despite the growing interest shown in these
materials, challenges such as the partial understanding of standardized measurement protocols,
and the relationship between the structure and optoelectronic properties still need to be
overcome.
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Critical assessment of predicted interactions at atomic resolutionMendez Giraldez, Raul 21 September 2007 (has links)
Molecular Biology has allowed the characterization and manipulation of the molecules of life in the wet lab. Also the structures of those macromolecules are being continuously elucidated. During the last decades of the past century, there was an increasing interest to study how the different genes are organized into different organisms (‘genomes’) and how those genes are expressed into proteins to achieve their functions. Currently the sequences for many genes over several genomes have been determined. In parallel, the efforts to have the structure of the proteins coded by those genes go on. However it is experimentally much harder to obtain the structure of a protein, rather than just its sequence. For this reason, the number of protein structures available in databases is an order of magnitude or so lower than protein sequences. Furthermore, in order to understand how living organisms work at molecular level we need the information about the interaction of those proteins. Elucidating the structure of protein macromolecular assemblies is still more difficult. To that end, the use of computers to predict the structure of these complexes has gained interest over the last decades.<p>The main subject of this thesis is the evaluation of current available computational methods to predict protein – protein interactions and build an atomic model of the complex. The core of the thesis is the evaluation protocol I have developed at Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Université Libre de Bruxelles, and its computer implementation. This method has been massively used to evaluate the results on blind protein – protein interaction prediction in the context of the world-wide experiment CAPRI, which have been thoroughly reviewed in several publications [1-3]. In this experiment the structure of a protein complex (‘the target’) had to be modeled starting from the coordinates of the isolated molecules, prior to the release of the structure of the complex (this is commonly referred as ‘docking’).<p>The assessment protocol let us compute some parameters to rank docking models according to their quality, into 3 main categories: ‘Highly Accurate’, ‘Medium Accurate’, ‘Acceptable’ and ‘Incorrect’. The efficiency of our evaluation and ranking is clearly shown, even for borderline cases between categories. The correlation of the ranking parameters is analyzed further. In the same section where the evaluation protocol is presented, the ranking participants give to their predictions is also studied, since often, good solutions are not easily recognized among the pool of computer generated decoys.<p>An overview of the CAPRI results made per target structure and per participant regarding the computational method they used and the difficulty of the complex. Also in CAPRI there is a new ongoing experiment about scoring previously and anonymously generated models by other participants (the ‘Scoring’ experiment). Its promising results are also analyzed, in respect of the original CAPRI experiment. The Scoring experiment was a step towards the use of combine methods to predict the structure of protein – protein complexes. We discuss here its possible application to predict the structure of protein complexes, from a clustering study on the different results.<p>In the last chapter of the thesis, I present the preliminary results of an ongoing study on the conformational changes in protein structures upon complexation, as those rearrangements pose serious limitations to current computational methods predicting the structure protein complexes. Protein structures are classified according to the magnitude of its conformational re-arrangement and the involvement of interfaces and particular secondary structure elements is discussed. At the end of the chapter, some guidelines and future work is proposed to complete the survey. / Doctorat en Sciences / info:eu-repo/semantics/nonPublished
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Extrakce a modifikace vlastností číslicových zvukových signálů v dynamické rovině / Digital Audio Signal Feature Extraction and Modification in Dynamic PlaneKramoliš, Ondřej January 2010 (has links)
This thesis deals with basic methods of root mean square and peak value measurement of a digital acoustic signal, algotithms to measure audio programme loudness and true-peak audio level according to recommendation ITU-R BS.1770-1 and digital systems for control of signal dynamic range. It shows achieved results of root mean square and peak value measurement and results of implementation of dynamic processor with general piecewise linear non-decreasing static curve and algorithms according to recommendation ITU-R BS.1770-1.
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Relative or Discounted Cash Flow Valuation on the Fifty Largest US-Based Corporations on Nasdaq : Which of these valuation methods provides the most accurate valuation forecast?Öhrner, Marcus, Öhman, Otto January 2023 (has links)
The topic of this Bachelor Thesis is “Which of these valuation methods provides the most accurate valuation forecast”. Assuming that the year is 2020, the goal of this thesis is to forecast the future stock prices of the fifty largest US-based companies on the Nasdaq stock exchange for 2021 and 2022. By using a quantitative method and looking ten years back at historical data. We determine which valuation method provides the most accurate stock price when conducted in a non-sector specific sample by comparing predicted prices to actual stock prices and discussing the results. There are several ways to evaluate a company and the ones being utilized in this thesis are the discounted cash flow valuation method, the price-to-earnings ratio method (equity multiple), and enterprise value to enterprise value before interest, tax, and depreciation (firm multiple). Our results show that when reviewing the valuations of multiple companies in different sectors the relative valuation methods provide better predictions with EV/EBITDA rather than the discounted cash flow method. This thesis provides the reader with a comprehensive overview of these different valuation methods and their effectiveness in providing valuation forecasts. The result of this thesis is beneficial for policymakers, investors, and financial analysts when forecasting future stock prices.
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Study On DC-Link Capacitor Current In A Three-Level Neutral-Point Clamped InverterGopalakrishnan, K S 07 1900 (has links) (PDF)
Three-level diode-clamped inverter is being widely used these days. Extensive research has been carried out on pulse width modulation (PWM) strategies for a three-level inverter. The most widely used PWM strategies are sine-triangle pulse width modulation (SPWM) and centered space vector pulse width modulation (CSVPWM). The influence of these PWM strategies on the DC-link capacitor current and voltage ripple is studied in this thesis.
The sizing of the DC capacitor depends on value of the maximum RMS current flowing through it. In this work, an analytical expression for capacitor RMS current is derived as a function of operating conditions like modulation index, power factor angle of the load and peak load current. The worst case current stress on the capacitor is evaluated using the analytical expression. The capacitor RMS current is found to be the same in SPWM and CSVPWM schemes. The analytical expression is validated through simulations and experiments on a 3kVA MOSFET based three-level inverter.
Harmonic analysis of the capacitor current is helpful in better evaluation of capacitor power loss. Therefore, harmonic analysis of the capacitor current is carried out, using the techniques of geometric wall model and double Fourier integral for SPWM and CSVPWM schemes. The theoretical predictions are validated through experiments.
The capacitor RMS current is divided into low-frequency RMS current (where low frequency component is defined as a component whose frequency is less than half the switching frequency) and high-frequency RMS current. The capacitor voltage ripple is estimated analytically for SPWM and CSVPWM schemes, using the low-frequency and high-frequency capacitor RMS current. The voltage ripples due to SPWM and CSVPWM schemes are compared. It is found that the voltage ripple with SPWM is higher than that with CSVPWM. A simplified method to estimate the capacitor power loss, without the requirement of FFT analysis of capacitor current, is proposed. The results from this simplified method agree reasonably well with the results from the detailed method.
A space vector based modulation scheme is proposed, which reduces the capacitor RMS current at high power factor angles. However, the proposed method leads to higher total harmonic distortion (THD) than CSVPWM. Simulation and experimental results, comparing CSVPWM and the proposed PWM, are presented.
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Měřič odstupu signálu od šumu obrazových signálů / BER Meter for Video SignalsŠimoník, Petr January 2008 (has links)
The diplomma thesis is dealing with possibilities of Signal to noise ratio measurement by method, which is based on direct measurement. It is chosen the most suitable method – signal and noise separation to two different parallel signal branches, where is measured signal strength in one branch and root mean square value in the other. The thesis is consisted of a concept of detail block scheme of Signal to noise ratio meter, which was designed in terms of theoretical knowledge. Particular functional blocks were circuit-designed, the active and passive parts were chosen and their function were described. There were made simulation and displayed input and output time flows. There is designed the whole connection of engineered Signal to noise ratio meter in the last part of my thesis. The double-sided board of printed circuit is contained too. It was created simple programme for supervisor micro-processor. Thereby were constructed complete bases for realization.
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Evaluation of Target Tracking Using Multiple Sensors and Non-Causal AlgorithmsVestin, Albin, Strandberg, Gustav January 2019 (has links)
Today, the main research field for the automotive industry is to find solutions for active safety. In order to perceive the surrounding environment, tracking nearby traffic objects plays an important role. Validation of the tracking performance is often done in staged traffic scenarios, where additional sensors, mounted on the vehicles, are used to obtain their true positions and velocities. The difficulty of evaluating the tracking performance complicates its development. An alternative approach studied in this thesis, is to record sequences and use non-causal algorithms, such as smoothing, instead of filtering to estimate the true target states. With this method, validation data for online, causal, target tracking algorithms can be obtained for all traffic scenarios without the need of extra sensors. We investigate how non-causal algorithms affects the target tracking performance using multiple sensors and dynamic models of different complexity. This is done to evaluate real-time methods against estimates obtained from non-causal filtering. Two different measurement units, a monocular camera and a LIDAR sensor, and two dynamic models are evaluated and compared using both causal and non-causal methods. The system is tested in two single object scenarios where ground truth is available and in three multi object scenarios without ground truth. Results from the two single object scenarios shows that tracking using only a monocular camera performs poorly since it is unable to measure the distance to objects. Here, a complementary LIDAR sensor improves the tracking performance significantly. The dynamic models are shown to have a small impact on the tracking performance, while the non-causal application gives a distinct improvement when tracking objects at large distances. Since the sequence can be reversed, the non-causal estimates are propagated from more certain states when the target is closer to the ego vehicle. For multiple object tracking, we find that correct associations between measurements and tracks are crucial for improving the tracking performance with non-causal algorithms.
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