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Extratos de algas marinhas como agentes antioxidantes e antimicrobianos e seus efeitos na qualidade de Minced de tilápia (Oreochromis niloticus) / Seaweeds extracts as antioxidants and antimicrobial agents and their effects on quality of tilapia Minced (Oreochromis niloticus)Ingridy Simone Ribeiro Cabral 04 May 2012 (has links)
A extração de Carne Mecanicamente Separada de tilápia tem se destacado como um processo atraente pela possibilidade de maior recuperação da carne após a filetagem. Porém, a separação mecânica aumenta a superfície de exposição, levando à incorporação de oxigênio e consequentemente ao \"off flavor\" devido à rancidez, tornando necessário o uso de aditivos para sua conservação. A tendência é utilizar produtos naturais como alternativas aos aditivos químicos. Entre os produtos naturais, as algas marinhas apresentam, em sua composição, metabólitos secundários com alta atividade antioxidante e antimicrobiana. Esta pesquisa teve como objetivo avaliar a composição química e a atividade biológica de quatro algas marinhas e seus efeitos, quando aplicados em Minced de tilápia. As algas Nori, Kombu, Hijiki e Wakame foram extraídas por 2 e 7 dias, em temperatura ambiente, com etanol 60, 80 e 100%. O teor de compostos fenólicos, a atividade antioxidante e a antimicrobiana in vitro foram determinados. A atividade antioxidante por métodos acelerados, Rancimat e Oxipres, também foi avaliada. As algas bioativas tiveram seu perfil químico elucidado por cromatografia líquida e gasosa. Essas algas foram aplicadas em Minced de tilápia, que, em seguida, foi armazenado a -18ºC por 180 dias. No Minced foram analisadas a composição centesimal, características de frescor por pH, BNVT e TBARS; cor instrumental, cor e aroma (de ranço) pela análise sensorial, padrões microbiológicos e composição de ácidos graxos, nos tempos 0, 60, 120 e 180 dias de armazenamento. Observou-se que a extração de 2 dias, com etanol 60%, foi a mais eficiente para extrair os compostos fenólicos. As algas Nori e Hijiki apresentaram os maiores teores desses compostos e, consequentemente, maior atividade antioxidante in vitro. Os extratos de algas não apresentaram atividade antimicrobiana contra Salmonella Enteritidis, Escherichia coli e Staphylococcus aureus. Para Klebsiella pneumoniae e Listeria monocytogenes, os extratos mais eficientes foram os extraídos com etanol 100%. As algas Nori e Hijiki foram selecionadas como as mais bioativas e submetidas aos testes de oxidação acelerada. Quando aplicadas em óleo de soja, no Rancimat, e em Minced de tilápia, no Oxipres, estas algas demonstraram efetiva atividade antioxidante. Pela cromatografia, os principais compostos identificados foram, na Nori, os ácidos clorogênico, vanílico e caféico, além dos aminoácidos alanina, glicina, valina e prolina; bem como, glicose e sorbitol. Na alga Hijiki foram detectados os ácidos clorogênico, caféico e cinâmico; alanina e prolina, bem como, xilitol e ribitol, frutose e ácido linoléico. No teste de armazenamento congelado do Minced, por 180 dias, a aplicação dos extratos de Nori e Hijiki não infuenciou na composição centesimal e pH. As algas mostraram-se eficientes no controle da produção das BNVT. No entanto, por TBARS, apesar de estarem dentro dos limites aceitáveis, as algas apresentaram comportamento pró-oxidante. Sob os aspectos microbiológicos, os minceds atenderam à legislação vigente. Sensorialmente, os provadores não detectaram diferença no \"aroma de ranço\", apenas mínimas diferenças na cor do produto. Pode-se concluir que o Minced de tilápia, adicionado de algas marinhas, manteve-se dentro dos padrões de qualidade durante o armazenamento congelado. Ressalta-se, ainda, a diferença na resposta antioxidante de acordo com o método utilizado / The extraction of minced tilapia has emerged as an attractive process due to the possibility of greater recovery of the tissue after filleting. However, mechanical separation increases the surface exposure, leading to incorporation of oxygen and consequently to the \"off-flavor\" due to rancidity, making necessary the use of additives for its preservation. The tendency is to use natural products as alternatives to chemical additives. Among the natural products, seaweeds present in its composition secondary metabolites with high antioxidant and antimicrobial activities. This research aimed to evaluate the chemical composition and biological activities of four seaweeds varieties and their effects when applied to the tilapia Minced. Nori, Kombu, Hijiki and Wakame seaweeds were extracted by 2 and 7 days, at room temperature, with ethanol 60, 80 and 100%. The phenolic content, antioxidant and antimicrobial activities in vitro were determined. The antioxidant activity by accelerated methods, Oxipres and Rancimat, were also evaluated. Seaweeds bioactive profiles were elucidated by liquid and gas chromatography. These seaweeds were applied to the tilapia Minced, that was stored at -18 °C for 180 days. The Minced were analyzed as to their composition, freshness characteristics by pH, TBARS and TVB-N, instrumental color, color and rancidity aroma by sensory analysis, microbiological standards, and fatty acid composition, at 0, 60, 120 and 180 days of storage. It was observed that the extraction after two days, with 60% ethanol, was the most efficient way to extract the phenolic compounds. Hijiki and Nori showed the highest levels of these compounds and therefore higher antioxidant activity in vitro. The extracts showed no antimicrobial activity against Salmonella Enteritidis, Escherichia coli and Staphylococcus aureus. For Klebsiella pneumoniae and Listeria monocytogenes, more efficient extracts were extracted with ethanol 100%. Hijiki and Nori seaweeds were selected as the most bioactive and submitted to accelerated oxidation tests. When applied in soy oil in the Rancimat, and tilapia Minced, in the Oxipres, these seaweeds have demonstrated effective antioxidant activity. By chromatography, the most important compounds identified were, in Nori, chlorogenic, caffeic and vanillic acids, besides the amino acids alanine, glycine, proline and valine, glucose and sorbitol. In the Hijiki seaweed, it was detected chlorogenic, caffeic and cinnamic acids, alanine and proline, as well as, ribitol and xylitol, fructose, and linoleic acid. In the test of frozen Minced storage, for 180 days, the application of Nori and Hijiki extracts did not modify neither the composition nor the pH. Seaweeds were effective in controlling the TVB-N production, but, for TBARS, although they were within acceptable limits, the seaweeds showed pro-oxidant activities. As to the microbiological aspects, the minceds complied with legal requirements. Sensorially, the tasters detected no difference in the \"rancid aroma\", only small differences in the product color. It can be concluded that the Minced tilapia, seaweed added, remained within the standards of quality during frozen storage. It should be noted, moreover, the difference in the antioxidant response, according to the method used.
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Atividade de neutrofilos e estresse oxidativo em cordeiros infectados experimentalmente com Haemonchus contortus e suplementados com selênio e vitamina E / Neutrophils activity and oxidative stress of experimentally infected lambs by Haemonchus contortus and supplemented with selenium and vitamin ECamargo, Emmanuel Veiga de 22 February 2010 (has links)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The present work had as its objective to assess the oxidizing metabolism of neutrophils, the complete blood count (CBC) and the oxidizing profile of experimentally infected lambs with Haemonchus contortus and supplemented with selenium and vitamin E. 20 male lambs were used, of the Corriedale breed, distributed in four experimental groups with 5 animals: G1 larvae infected animals and supplemented with 0.2mg/kg living weight (PV) of selenite via intramuscular (IM); G2 larvae infected animals and supplemented with 0.2mg/kg PV of selenite IM and 2000 UI per animal of vitamin E IM; G3 larvae infected animals and supplemented with 2000 UI per animal of E IM; G4 larvae infected animals. For the CBC and the biochemical analysis blood collection on the day zero (T0) were carried out, 20 (T1), 40 (T2) and 60 (T3) through jugular venipuncture using vacutainer® tubes. The weighing and the egg determination by gram of animal feces occurred in the same experimental times. For the NBT tests heparinized blood samples were collected in the days zero, 30, 60. Smaller leukocyte values were detected in the supplemented group exclusively with selenium (G1) in relation to the control group (G4) in time 4. Concerning the lymphocytes it was observed a decrease in the G1 in relation to the supplemented only with vitamin E (G3) and G4 in time 3 (T3). For both the tests, NBT-NE and NBT-E there was a decrease in the dye reduction capacity at 60 days in relation to the other times in the groups treated with selenium (G1 and G2). The result of the oxidizing profile showed a significant elevation in the GSH-Px enzyme in the supplemented groups with selenium. Still, the correlation of Pearson revealed the existence of a negative correlation between the concentrations of GSH-Px and TBARS and between this enzyme and the OPG values. Increments were also observed for the catalase enzyme in animal which received the selenium supplementation or when this element was associated to the vitamin E. Smaller values of lipid peroxidation (TBARS) were detected in supplemented animals when these were compared to the control group. These results suggest that the reduced reserves of this antioxidant may exacerbate the generation of free radicals with an increase of the lipid peroxidation and the increase of the environment contamination by the eggs of Haemonchus contortus. Facing these results it is possible to conclude that the selenium supplementation provides a larger protection to the cell antioxidant and to the organism as a whole in lambs experimentally infected by Haemonchus contortus. / O presente trabalho teve como objetivo avaliar o metabolismo oxidativo dos neutrófilos, o hemograma e o perfil oxidativo de cordeiros experimentalmente infectados com Haemonchus contortus e suplementados com selênio e vitamina E. Foram utilizados 20 cordeiros machos, da raça Corriedale, distribuídos em quatro grupos experimentais com 5 animais: G1- animais infectados com larvas e suplementados com 0,2mg/kg de peso vivo (PV) de selenito de sódio por via intramuscular (IM); G2- animais infectados com larvas e suplementados com 0,2mg/kg PV de selenito de sódio IM e 2000 UI por animal de Vitamina E IM; G3- animais infectados com larvas e suplementados com 2000 UI por animal de Vitamina E IM; G4-animais infectados com larvas. Todos os grupos foram infectados, pela via oral, com 500 larvas L3 de Haemonchus contortus por animal a cada dois dias, pelo período de vinte dias a partir do dia zero. Para o hemograma e as análises bioquímicas foram realizadas coletas de sangue nos dias zero (T0), 20 (T1), 40 (T2) e 60 (T3) por venopunção da jugular utilizando-se tubos vacutainer®. As pesagens e a determinação dos ovos por grama de fezes dos animais ocorreram nesses mesmos tempos experimentais. Para as provas de NBT foram coletadas amostras de sangue heparinizadas nos dias zero, 30 e 60. Menores valores de leucócitos totais foram detectados no grupo suplementado exclusivamente com selênio (G1) em relação ao grupo controle (G4) no tempo 4. Em relação aos linfócitos observou-se diminuição no G1 em relação ao suplementado somente com vitamina E (G3) e G4 no tempo 3 (T3). Para ambos os testes, NBT-NE e NBT-E houve uma diminuição da capacidade de redução do corante aos 60 dias em relação aos demais tempos nos grupos tratados com selênio (G1 e G2).Os resultados do perfil oxidativo demonstraram significativa elevação da atividade da enzima GSH-Px nos grupos suplementados com selênio. Ainda, a correlação de Pearson revelou a existência de correlação negativa entre as concentrações de GSH-Px e TBARS e entre esta enzima e os valores de OPG. Incrementos também foram observados para a enzima catalase nos animais que receberam suplementação com selênio ou quando este elemento foi associado a vitamina E. Menores valores de lipoperoxidação lipídica (TBARS) foram detectados nos animais suplementados quando estes foram comparados ao grupo controle. Esses resultados sugerem que as reservas diminutas deste antioxidante podem exacerbar a geração de radicais livres com um aumento da peroxidação lipídica e o aumento da contaminação ambiental pelos ovos de Haemonchus contortus. Diante dos resultados é possível concluir que a suplementação com selênio proporciona uma maior proteção antioxidante celular e ao organismo como um todo de cordeiros experimentalmente infectados pelo Haemonchus contortus.
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Efeito do pH e dureza da água em juvenis de Rhamdia quelen infectados com Ichthyophthirius multifiliis (Fouquet, 1876) / Effect of water pH and hardness in Rhamdia quelen juveniles infected with Ichthyophthirius multifiliis (Fouquet, 1876)Garcia, Luciano de Oliveira 20 February 2008 (has links)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The aim of this study was to determine the intensity of Ichthyophthirius multifiliis infection, as well as net
ion fluxes (Na+, K+ and Cl-), in silver catfish juveniles exposed to different pHs (5, 6, 7, 8, and 9 for
sixteen days), pH (5.0 and 7.0) and hardness (20, 60 and 120 mg CaCO3.L-1 for sixteen days) and the
oxidative stress parameters in liver, gill and muscle of this species and submitted to different pH (5.0 and
7.0 for three days). Net Na+, K+, and Cl- fluxes were determined at different times, trophonts in the skin
and gill were counted, and mortality was registered daily. After six days fish kept at pH 6.0, 7.0, 8.0 and
9.0-hardness 20 mg CaCO3.L-1 showed significantly higher cumulative mortality (100% after eight days)
and number of trophonts on the skin and gill compared to pH 5.0-hardness 20 mg CaCO3.L-1. Infected
silver catfish showed significantly higher Na+ and K+ effluxes in the first day, and there was a recovery
(influx) after the second day compared to asymptomatic juveniles. Silver catfish juveniles infected with
I.multifiliis and exposed to pHs 5.0 and 7.0 presented significantly higher TBARS levels in the liver and
gills compared to asymptomatic juveniles. The activity of catalase in the liver of silver catfish juveniles
infected and exposed to both pHs was significantly lower (1st and 3rd day) than in asymptomatic
juveniles. The GST activity in the liver and gills of infected juveniles increased throughout all
experimental period compared to asymptomatic juveniles. The muscle of infected juveniles maintained at
pH 5.0 showed significantly lower TBARS levels at day three compared to asymptomatic juveniles. The
CAT activity was significantly lower in the muscle of infected juveniles at pH 5.0 and 7.0 at all
experimental days except day 1 at pH 7.0 compared to asymptomatic juveniles. The muscle of infected
juveniles presented significantly lower GST activity in all experimental period at both pH 5.0 and 7.0
compared to asymptomatic juveniles. These results allowed us to conclude that infection by I. multifiliis is
less severe in silver catfish maintained at pH 5.0-hardness 20 mg CaCO3.L-1. Increase of water hardness
increases trophonts infection and impairs survival in silver catfish kept at pH 5.0, but the opposite is
observed when juveniles are at pH 7.0. There was no clear evidence of a relationship between mortality
and trophonts number in infected silver catfish with net ion fluxes. Infection with I. multifiliis induces
liver and gill damage via lipid peroxidation products, but the same is not observed in the muscle. / O objetivo deste estudo foi determinar a intensidade da infecção pelo Ichthyophthirius multifiliis, assim
como o fluxo líquido de íons (Na+, K+ e Cl-), em juvenis de jundiá expostos a diferentes pHs (5,0; 6,0; 7,0;
8,0 e 9,0 por dezesseis dias), pH (5,0 e 7,0) e dureza (20, 60 e 120 mg CaCO3/L por dezesseis dias) e os
parâmetros de estresse oxidativo no fígado, brânquias e músculo nesta espécie e submetida a diferentes
pHs (5,0 e 7,0 por 3 dias). O fluxo dos íons Na+, K+ e Cl- foi determinado em diferentes tempos, o número
de trofontes na pele e nas brânquias foi contado e a mortalidade foi registrada diariamente. Após seis dias
os peixes submetidos aos pHs 6,0; 7,0; 8,0 e 9,0-dureza de 20 mg CaCO3/L apresentaram mortalidade
cumulativa (100% após oito dias) e numero de trofontes na pele e nas brânquias significativamente maior
que os mantidos em pH 5,0-dureza de 20 mg CaCO3/L. Jundiás infectados apresentaram efluxo de Na+ e
K+ significativamente maior no primeiro dia, havendo uma recuperação (influxo) a partir do segundo dia
em relação aos juvenis assintomáticos. Juvenis de jundiá infectados com I.multifiliis e expostos aos pHs
5,0 e 7,0 apresentaram significativo aumento dos níveis de TBARS no fígado e nas brânquias em relação
aos juvenis assintomáticos. A atividade da catalase no fígado dos juvenis de jundiás infectados e expostos
a ambos pHs foi significativamente maior e menor (1º e 3º dia), em relação aos juvenis assintomáticos. A
atividade da GST no fígado e nas brânquias aumentou durante todo o período experimental em relação aos
juvenis assintomáticos. O músculo dos juvenis infectados e mantidos em pH 5,0 apresentou significativa
diminuição nos níveis de TBARS no terceiro dia comparado aos juvenis assintomáticos. A atividade da
catalase foi significativamente menor no músculo dos juvenis infestados e submetidos ao pH 5,0 e 7,0 em
todos os dias experimentais, exceto no primeiro dia em pH 7,0 quando comparada aos juvenis
assintomáticos. O músculo dos juvenis infectados apresentou atividade da GST significativamente menor
em todo o período experimental em ambos pH 5,0 e 7,0 quando comparados aos juvenis assintomáticos.
Estes resultados nos permitem concluir que a infecção pelo I. multifiliis é menos severa em jundiás
mantidos em pH 5,0-dureza de 20 mg CaCO3/L. O aumento da dureza da água aumenta a infecção pelos
trofontes e afeta a sobrevivência dos jundiás mantidos em pH 5,0, mas o oposto é observado quando os
juvenis estão no pH 7,0. Não houve uma evidência clara da relação entre a mortalidade e o número de
trofontes nos juvenis de jundiá infectados com o fluxo líquido de íons. A infecção por I. multifiliis induz
danos no fígado e brânquias, via produtos da peroxidação lipídica, o mesmo não ficando evidenciado no
músculo.
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Physiochemical and Rheological Properties of Alkaline Isolated Poultry ProteinsMoayedi Mamaghani , Vida Unknown Date
No description available.
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Physiochemical and Rheological Properties of Alkaline Isolated Poultry ProteinsMoayedi Mamaghani , Vida 06 1900 (has links)
Chicken dark meat has been considered as a major underutilized commodity due to the increasing demand for further processed breast meat products. Alkali aided protein extraction is an option to increase the utilization of chicken dark meat. First, the effect of pH (10.5-12.0) on alkaline extraction of chicken dark meat has been studied, and protein yield, composition, color, and TBARs of the extracted meat have been determined. Second, textural and rheological properties and water holding capacity (WHC) of alkali extracted chicken dark meat have been evaluated. The highest protein yield (94.2%) was obtained at pH 12.0. Lipid content of the extracted meat decreased by 50% compared to chicken dark meat. WHC, hardness and chewiness of extracted meat were greater at higher pH. The gel from recovered meat with added cryoprotectants showed more stability. This process may offer the possibility to use the underutilized poultry resources for preparation of functional foods. / Food Science and Technology
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Estresse oxidativo em pacientes beta talassêmicos heterozigotos e com deficiência de glicose-6-fosfato desidrogenaseOndei, Luciana de Souza [UNESP] 28 August 2009 (has links) (PDF)
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000603676.pdf: 889558 bytes, checksum: df390d92da0411515e31635f99e1d76d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Ministério da Saúde / Na talassemia beta, o acúmulo das cadeias alfa livres, bem como a liberação do grupo heme e do ferro durante o processo hemolítico, ocasionam aumento de danos oxidativos que podem resultar em lipoperoxidação de membranas celulares, desnaturação de proteínas e oxidação da hemoglobina. Na deficiência de glicose- 6-fosfato desidrogenase (G6PD), esse aumento é decorrente da diminuição da produção de nicotinamida adenina dinucleotídeo fosfato reduzido (NADPH) que pode resultar em hemólise intravascular. Diante da possibilidade de estresse oxidativo nos portadores de beta talassemia heterozigota e nos indivíduos com deficiência de G6PD, neste trabalho avaliou-se a expressão fenotípica das afecções genéticas por meio da identificação das mutações e análise de marcadores para estresse oxidativo. Para o estabelecimento dos grupos controle e com deficiência de G6PD foram avaliadas 544 amostras de sangue periférico de indivíduos da região Noroeste do Estado de São Paulo, sendo 426 doadores de sangue e 118 indivíduos de uma instituição de ensino superior. Para a composição do grupo com talassemia beta heterozigota foram avaliadas 46 amostras de sangue de indivíduos com diagnóstico clínico de talassemia beta da cidade de São Carlos/SP. Foram realizados métodos de triagem e confirmatórios para a identificação da talassemia beta heterozigota e da deficiência de G6PD, e dosagens bioquímicas para quantificação das espécies reativas ao ácido tiobarbitúrico (TBARS), utilizado como marcador de estresse oxidativo, e para a determinação da capacidade antioxidante em equivalência ao Trolox (TEAC). Os polimorfismos da glutationa S-transferase (GST) GSTM1 e GSTT1 foram avaliados por PCR multiplex o de GSTP1 por PCR/RFLP. No grupo com talassemia beta heterozigota foram encontradas 18 (39%) amostras com a mutação CD39; 22 (48%) com a mutação... / In beta thalassemia, the excess of unpaired alpha chains, as well as the heme group and iron released during the hemolytic process increase the oxidative damage. In G6PD deficiency, this increase is caused by a reduced production of NADPH that results in an intravascular hemolysis. Thus, facing the oxidative stress possibility in beta thalassemia carriers and G6PD deficiency individuals, it was aimed to evaluate the fenotypic expression of this genetic disorders through the mutation identification, as well as the oxidative stress marker analysis. We used 544 peripheral blood samples of individuals from São Paulo’s northwestern to control group and to G6PD deficiency group establishment. For beta thalassemia heterozygote group were evaluated 48 blood samples of São Carlos/SP city. Tests were carried out aiming the screening and confirmation of beta thalassemia and G6PD deficiency, as well as the analysis of lipid peroxidation products measured as thiobarbituric acid reactive species (TBARS) and Trolox equivalent antioxidant capacity (TEAC). Were determined the frequencies of GSTM1, GSTT1 and GSTP1 polymorphisms. The analysis with beta thalassemia carriers allowed to establish in the study group a frequency of 39% for CD39 mutation, 48% for IVS-I-110 mutation and 2% for IVS-I-6 mutation. For G6PD deficiency was founded a frequency of 3.86%. The beta thalassemic group evaluation showed an increase of TBARS and TEAC values, when compared to the control group. There was a tendency to increase lipid peroxidation in beta0 CD39 mutants compared to beta+ IVS-I-110 mutants, because there is more free chains amount in beta0 thalassemia than beta+ thalassemia. In the G6PD deficiency analysis was found a lower G6PD activity in men than in women, but there was no interference of gender in the TBARS and TEAC assays results. The comparison between the control group and the G6PD deficiency group... (Complete abstract click electronic access below)
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Envolvimento da proteína, carboidrato, lipídio e selênio sobre as alterações metabólicas e bioquímicas em frangos submetidos ao calorHada, Fabricio Hirota [UNESP] 15 August 2008 (has links) (PDF)
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hada_fh_me_jabo.pdf: 456451 bytes, checksum: 872a572717a21550d72964f8a222f7fa (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo do trabalho foi averiguar quais seriam as possíveis alterações metabólicas e bioquímicas, principalmente relacionadas à capacidade antioxidante muscular, quando frangos de corte são submetidos a diferentes alterações nos macronutrientes e da adição do selênio na dieta, bem como o desempenho de frangos de corte ao serem submetidas ao estresse térmico de calor de forma aguda. Foram utilizados pintos de corte de um dia de idade, criados até o 7º dia com dieta comercial, no 8° dia as aves foram submetidas às dietas experimentais. A alteração realizada na proteína causou maior influência sobre o desempenho e nos cortes comerciais, quando comparados com as alterações no carboidrato e lipídeo. A adição de selênio influenciou positivamente a viabilidade criatória em aves arraçoadas com baixa proteína, porém não influenciou o rendimento de carcaça, peito, coxa+sobre-coxa. Estas alterações causam alterações metabólicas e bioquímicas nos frangos, sendo que o nível protéico causou grande impacto sobre os níveis de triglicérides e ácido úrico. Frangos submetidos a estresse por calor apresentaram alterações nas concentrações dos metabólitos plasmáticos, e na atividade da catalase em aves arraçoadas com diferentes alterações na dieta. A adição de selênio não influenciou os parâmetros sanguíneos dos 14 aos 42 dias de idade, porém houve influência no ácido úrico aos 28 dias, triglicérides, ácido úrico e CK aos 35 dias e para glicose aos 42 dias quando as aves foram submetidas a estresse por calor. Mas não houve efeito sobre a catalase, superóxido dismutase e glutationa peroxidase, mas observou-se influência sobre o TBARS. / The objective of the work was to check out which would be the possible metabolic and biochemical alterations, mainly those concerning the muscular anti-oxidant capacity as a broiler chicken is submitted to different changes in the nutrients, and the addition of selenium in the diet, as well as the performance of the broiler chicken being submitted to an acute heating stress. It was used broilers with one day of life, raised up to their seventh day with a conventional diet at the eight; the birds were submitted to experimental diets. The change brought about in protein impacted somewhat the performance and the commercial cuts, if compared with the alterations in the carbohydrate and lipids. The selenium addition influenced positively the breeding viability in birds feed with low protein, however the influences over the carcass yield, trunk and legs were not significant. This different changes gave rise to both metabolic and biochemical in broiler. The protein levels offered great impact on the levels of uric acid, triglycerides. Broilers submitted to stress due to heat presented alterations in the concentrations of the plasmatic metabolites, and also in the catalase in birds fed with different alterations in their diets. The addition of the selenium didn’t influence the sanguineous parameters from the 14th to the 42nd days of age. However, there was a change in the uric acid at the 28 days, triglycerides, uric acid and CK at the 35th days. As to the glucose, at the 42nd days as the birds were stressed by the heat. However did not alter the catalase enzymes, superóxide dismutase and glutationa peroxidase but an influence was held once the TBARS.
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Utilização de extratos comerciais derivados de plantas em produtos cárneos : avaliação da atividade antioxidante / Plant-derived commercial extracts on meat products : study on the antioxidant activityPaglarini, Camila de Souza, 1989- 04 June 2015 (has links)
Orientador: Marise Aparecida Rodrigues Pollonio / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-27T13:09:44Z (GMT). No. of bitstreams: 1
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Previous issue date: 2015 / Resumo: Os produtos cárneos são muito susceptíveis à oxidação lipídica, uma das principais reações de deterioração e a causa principal de sabor e odor desagradáveis, redução do valor nutricional e da vida útil, além da formação de compostos tóxicos. O consumo excessivo de produtos cárneos está relacionado com o aumento do risco de algumas doenças, tais como doenças cardiovasculares, câncer, hipertensão e obesidade e assim pesquisas vem sendo desenvolvidas para elaboração de produtos mais saudáveis, dentre os quais destacam-se aqueles com redução de aditivos sintéticos. Neste contexto, o objetivo do presente estudo foi avaliar a atividade antioxidante de extratos comerciais derivados de plantas em matéria-prima cárnea (carne de frango mecanicamente separada - CFMS) e em produto cárneo reestruturado elaborado com diferentes matérias-primas (carne bovina, suína, de frango e CFMS). As concentrações dos extratos naturais foram de 0,125, 0,25, 0,5 e 1,0%, m/m. Os extratos foram caracterizados quanto ao teor de compostos fenólicos, flavonóides totais, atividade antioxidante ORAC, DPPH e ABTS. A oxidação lipídica foi avaliada pela análise de substâncias reativas ao ácido tiobarbitúrico - TBARS. A matéria-prima cárnea foi avaliada crua em ambiente refrigerado (4 °C) nos dias 0, 2, 4, 6, 8 e 10 e o produto cárneo foi avaliado cozido refrigerado (4 °C) nos dias 0, 3 e 6 e cru congelado (-18 °C) nos dias 0, 30 e 60 de vida útil. Quando caracterizados, todos os extratos naturais apresentaram atividade antioxidante, com destaque para os extratos de semente de uva e chá verde. Na CFMS todos os extratos apresentaram potencial antioxidante, sendo que o extrato de romã foi o menos efetivo e assim não foi aplicado no produto cárneo. O extrato de chá verde foi o mais efetivo contra a oxidação nos hambúrgueres cozidos e crus. No entanto os extratos de semente de uva, alecrim e mate também aumentaram a vida útil dos hambúrgueres cozidos. Nos hambúrgueres congelados a vida útil foi aumentada pelos extratos de semente de uva e alecrim. Os extratos naturais apresentaram maior potencial antioxidante nos hambúrgueres quando comparados com o antioxidante sintético BHT. Os resultados obtidos sugerem que extratos comerciais derivados de plantas podem ser utilizados como antioxidantes naturais em produtos cárneos, no entanto, estudos sensoriais tornam-se necessários para viabilizar sua adição. Com relação à oxidação lipídica, é possível a utilização de extratos comerciais derivados de plantas em produtos cárneos, melhorando a sua qualidade nutricional / Abstract: Meat products are very susceptible to lipid oxidation, a major degradation reaction and primary cause of off-flavors, reduction in the nutritional value and shelf life, and formation of toxic compounds. Increased consumption of meat products has been associated with a high risk of developing cardiovascular disease, cancer, hypertension and obesity. Therefore, several studies have focused on the manufacture of healthier products, among which the products with less synthetic additives have stood out. In this context, the aim of this study was to evaluate the antioxidant activity of plant-derived commercial extracts on meat raw materials (mechanically separated poultry - MSP) and restructured meat product made with different raw materials (beef or pork or chicken or MSP). The natural extracts concentrations were 0.125, 0.25, 0.5, and 1.0% w / w. The extracts were characterized for phenolics content, total flavonoids, and antioxidant activity using ORAC, DPPH, and ABTS assays. Lipid oxidation was evaluated by measuring thiobarbituric acid reactive substances - TBARS. The chilled (4 °C) fresh raw material was evaluated at days 0, 2, 4, 6, 8, and 10, while the cooked product (4 °C) was evaluated at days 0, 3, and 6, and the fresh frozen product (-18 °C) was evaluated at days 0, 30 and 60 of storage. All natural extracts exhibited antioxidant activity, especially the grape seed and green tea extracts. In the MSP, all extracts presented antioxidant potential, and the pomegranate extract was less effective and therefore has not been used in the product formulation. The green tea extract was the most effective against oxidation in cooked and raw burgers. However, the grape seed, rosemary, and mate extracts also increased shelf life of the cooked burgers. With respect to the frozen hamburgers, the shelf life was also increased by grape seed and rosemary extracts. A higher antioxidant potential of the natural extracts was observed when compared to the synthetic antioxidant BHT. The results suggest that despite commercial plant-derived extracts can be used as natural antioxidants in meat products, sensory studies are necessary to enable their addition. With regard to lipid oxidation, it is possible to use commercial plant-derived extracts in meat products, improving nutritional quality / Mestrado / Tecnologia de Alimentos / Mestra em Tecnologia de Alimentos
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Einfluss von Moderat-Intensivem Kontinuierlichem Ausdauertraining und Hochintensivem Intervalltraining auf die HDL- Funktion bei Patienten mit Herzinsuffizienz mit erhaltener linksventrikulärer Ejektionsfraktion (HFpEF)Sowa, Pamela Weronika 12 April 2022 (has links)
Hintergrund: Bei der Herzinsuffizienz mit erhaltener Ejektionsfraktion (HFpEF) handelt es sich um ein komplexes Krankheitsbild, das mit steigenden Hospitalisierungsraten an immer zunehmender Bedeutung im Gesundheitssystem gewinnt. Trotz hoher Mortalitätsraten hat sich keine Standardtherapie etabliert und bisher existieren keine erfolgreichen Behandlungsmaßnahmen. Zudem macht die HFpEF die Hälfte aller Herzinsuffizienzfälle aus, was die Zweckmäßigkeit der Erforschung einer effektiven Therapie hervorhebt. Als ein der entscheidenden Faktoren in der Pathophysiologie der Erkrankung wird die endotheliale Störung gestellt. Mit Reduktion der NO-Bioverfügbarkeit im Endothelium führt die endotheliale Störung zur LV -Versteifung mit diastolischer Dysfunktion des Herzens. Das körperliche Training und daraus resultierende Scherkräfte im Blutgefäß triggern eine HDL-vermittelte Stickstoffmonoxid Synthese. Die vasodilatative, eNOS-steigernde Funktion des HDL kann bei Herzinsuffizienten gestört sein. Eine entsprechende Trainingsmodalität könnte sich jedoch als Endothelium-schützend zeigen. Fragestellung: Ziel dieser Dissertation ist die Erforschung, ob die HDL-Funktion von Patienten mit HFpEF durch das Hochintensive Intervalltraining (90-95 % der maximalen HF) oder Moderat-Intensives Kontinuierliches Ausdauertraining (60-70 % der maximalen HF) beeinflussbar ist. Im Fokus der Untersuchungen stehen die HDL-gesteuerte eNOS- Funktion (Funktion der endothelialen Stickstoffmonoxid-Synthase), die durch die Phosphorylierung an unterschiedlichen Aminosäureresten im Molekül ausgewertet wird, die PON-1 Aktivität (Paraoxonase-1), die für anti-oxidative Rolle des HDL spricht, sowie die Konzentrationen von TBARS (Thiobarbitursäure-reaktive Substanzen), die als Indikatoren der gesteigerten Lipidperoxidation dienen. Material und Methoden: Um die Fragen zu klären, wurden die Probanden in drei Gruppen randomisiert: 1) HIIT (Hochintensives Intervalltraining), 2) MCT (Moderat-Intensives Kontinuierliches Ausdauertraining) und 3) CG (Kontrollgruppe). Vor, während und nach den verschiedenen Trainingsinterventionen wurde das HDL durch Ultrazentrifugation aus dem Blutserum der Probanden isoliert. Im Anschluss daran erfolgte die Inkubation der gewonnenen HDL-Isolate mit humanen Aortenendothelzellen. Mittels Western Blot wurde die HDL-induzierte eNOS-Phosphorylierung an der aktivierenden Position Ser1177 (die zur Steigerung der eNOS-Aktivität führt) und an der deaktivierenden Position Thr 495 (die zur Hemmung der eNOS-Aktivität führt) ermittelt. Aktivierende Phosphorylierung der eNOS geht mit einer gesteigerten NO-Produktion einher. Zur Klärung, wie weit modifizierbar die Endothelium-schützende Rolle des HDL ist und welche Korrelationen damit einhergehen, wurde anti-oxidative Funktion des HDL gemessen. Dazu diente die Messung der Aktivität des assoziierten Enzymes - Paraoxonase-1 (PON-1), das die Lipoproteine vor oxidativer Modifikation schützt. Die protektive Eigenschaft des HDL lässt sich unter anderem durch reaktive Sauerstoffspezies modifizieren. Die reduzierte PON-1 Aktivität kann mit gesteigerter Lipidperoxidation mit daraus entstehenden Fettsäure-Radikale einhergehen. Als Indikator wurde in der vorliegenden Studie die Konzentration an Thiobarbitursäure- reaktiver Substanzen (TBARS) gemessen. Ergebnisse/ Beobachtungen: Durch das körperliche Training konnte man einen Anstieg der eNOS-Phosphorylierung an Ser1177 hervorrufen. Nach dem ersten supervidierten Teil des HIIT kam es zu einer signifikanten HDL-induzierten eNOS-Phosphorylierung an Ser 1177. Weder nach HIIT noch nach MCT konnte jedoch ein signifikanter Rückgang der eNOS Phosphorylierung an der deaktivierenden Position Thr495 festgestellt werden. Bei der Betrachtung der eNOS-Phosphorylierung in der MCT- und Kontrollgruppe fielen keine signifikanten Unterschiede auf. Bei HIIT Probanden ergaben sich signifikant erhöhte Werte von PON-1 Aktivität sowohl im Serum als auch im HDL. In MCT- und Kontrollgruppe konnten hingegen keine signifikante Steigerung der PON-1 Aktivität nachgewiesen werden. Bei der Betrachtung von erhobenen Daten dieser Arbeit fallen erhöhte Werte von TBARS- Konzentrationen bei allen Probanden auf. Es kam allerdings zu keinen signifikanten Unterschieden nach den Trainingsinterventionen. Schlussfolgerungen: Die vorliegende Studie liefert die Erkenntnisse, dass das Hochintensive Intervalltraining eine signifikant gesteigerte HDL-vermittelte eNOS Phosphorylierung, die ebenfalls mit signifikant erhöhter PON-1 Aktivität einhergeht, bei HFpEF-Erkrankten induziert. Ferner aus den erhobenen Daten lässt sich ableiten, dass die Compliance bei allen Probandengruppe jeweils höher während eines überwachten Trainings als während einer home-basierten Intervention war. Insgesamt kann man daraus spekulieren, dass HIIT eine verbesserte endotheliale Funktion des HDL hervorrufen könnte und eine mögliche effektive Therapiemethode der HFpEF in Zukunft darstellen würde. Eine Nutzung dieser Methode bleibt offen und bedarf weiterer Forschungen.:Inhaltsverzeichnis I
Tabellenverzeichnis IV
Abbildungsverzeichnis V
Abkürzungsverzeichnis VI
1 Einleitung 3
1.1 Die Herzinsuffizienz mit erhaltener Ejektionsfraktion 3
1.1.1 Definition der Herzinsuffizienz, Klinisches Bild, Einteilung nach der Pathophysiologie 3
1.1.2 Epidemiologie der HFpEF 3
1.1.3 Pathomechanismus der HFpEF 4
1.1.4 HFpEF vs. HFrEF 11
1.1.5 Diagnose der HFpEF 12
1.1.6 Behandlungsmöglichkeiten 12
1.2 Körperliches Training 14
1.2.1 Belastungsintoleranz bei HFpEF 14
1.2.2 Hochintensives Intervalltraining (HIIT) und Moderat-Intensives
Kontinuierliches Ausdauertraining (MCT) 15
1.3 High density lipoproteins 16
1.3.1 HDL bei Herzinsuffizienz 16
1.3.2 Funktionen und Bedeutung von HDL-Partikeln 17
1.4 Zielsetzung und Fragestellung 19
2 Material 20
2.1 Serum 20
2.2 Zellkulturmaterial 20
2.3 Kulturmedium 20
2.4 Chemikalien und Lösungen 20
2.5 Färbelösungen 20
2.6 Antikörper 20
2.7 Chemilumineszenz 20
2.8 Sonstige Materialien 21
2.9 Geräte 21
2.10 Software 21
3 Methoden 22
3.1 Studiendesign 22
3.2 Trainingsintervention 23
3.3 Isolation des HDL 24
3.3.1 Vorarbeiten 24
3.3.2 HDL Isolation 24
3.3.3 HDL Aufreinigung 25
3.4 Bestimmung der Proteinkonzentration nach der BCA-Methode 25
3.5 Stimulation der Zellen 26
3.6 Western Blot 26
3.7 Gelelektrophorese 27
3.8 Proteintransfer 28
3.9 Immundetektion 29
3.10 Chemilumineszenz 29
3.11 PON-1 Aktivität 30
3.12 TBARS 30
3.13 Statistische Analyse 30
4 Ergebnisse 31
4.1 Charakterisierung der Patienten 31
4.2 Patientendaten 32
4.3 Qualität des isolierten HDL 38
4.4 Die HDL-induzierte eNOS-Phosphorylierung 40
4.5 Aktivität der Paraoxonase 1 (PON-1) 44
4.6 TBARS-Konzentration im Serum 47
5 Diskussion 49
5.1 Hauptaussagen 49
5.1.1 Endotheliale Effekte des Trainings via HDL 50
5.1.2 Trainingsmodalitäten als potentielle Therapie 52
5.1.3 Denkbarer molekularer Mechanismus für die Regulation von
endothelialen Funktionen des HDL 55
5.1.4 Quantitative vs. qualitative Auswertung der HDL Funktion 57
5.1.5 Unterschiede bei der Einhaltung der verschiedenen
Trainingsprotokollen 59
5.1.6 Ausblick für zukünftige Forschungsarbeiten 61
5.1.7 Studienlimitationen 63
6 Zusammenfassung 65
7 Summary 67
8 Literaturverzeichnis 69
9 Anlage 1 88
10 Anlage 2 89
11 Curriculum vitae 90
12 Danksagung 91
Tabellenverzeichnis
Tabelle 1: Reagenzien zur Vorbereitung eines 8 % und 12 % SDS-Polyacrylamidgels 27
Tabelle 2: Die verwendeten Antikörper zur Detektion der Proteine 29
Tabelle 3: Patientendaten zu Beginn der Studie -V1 32
Tabelle 4: Patientendaten zu den Zeitpunkten V1, V2, V3 33
Tabelle 5: Medikation 34
Tabelle 6: Lipidprofil 36
Tabelle 7: Ergospirometrie 37
Abbildungsverzeichnis
Abbildung 1: Pathomechanismus der myokardialen Dysfunktion (übernommen aus Paulus & Tschöpe, 2013) 6
Abbildung 2: Entstehung der myokardialen Dysfunktion durch Veränderungen innerhalb extrazellulärer Matrix und Kardiomyozyten bei Übergewicht und Diabetes mellitus (DM) 7
Abbildung 3: Komorbiditäten bei HFpEF als Trigger für diastolische Dysfunktion 9
Abbildung 4: NO Synthese als Antwort auf Shear Stress 10
Abbildung 5: Schematische Darstellung, wie ein körperliches Training das Krankheitsfortschreiten sowie HDL-induzierte NO-Produktion beeinflusst (übernommen aus Adams et al., 2013) 18
Abbildung 6: Studiendesign 22
Abbildung 7: Trainingsprotokoll, MCT-Moderat-Intensives Kontinuierliches Ausdauertraining, HIIT- Hochintensives Intervalltraining, HRpeak- maximale Herzfrequenz (übernommen aus Suchy et al., 2014) 23
Abbildung 8: Positionieren der Kanülenspitze und Entnahme des HDL 25
Abbildung 9: SDS-Page nach Färbung mit Coomassie-Blau zum Nachweis von HDL mit Größenmarker, BSA-Standard und den HDL-Proben (10 μg, 20 μg und 30 μg des Proteins) 38
Abbildung 10: Die Immundetektion der gebundenen Antikörper Apo-Protein A 1 durch eine Chemilumineszenz-Reaktion mit Größenmarker (M) zum Nachweis von HDL. Apo-AI, das Hauptprotein des HDL, mit einer Molekülgröße von 28 kDa wurde in 1,95 μg, 0,99 μg, 0,5 μg und 0,099 μg des HDL-Isolates identifiziert 39
Abbildung 11: Die eNOS-Phosphorylierung an Ser1177 zu den Zeitpunkten V1, V2, V3 bei der HIIT-(C), MCT-(B) und CG-Gruppe-(A) 40
Abbildung 12: Die eNOS-Phosphorylierung an Thr495 zu den Zeitpunkten V1, V2, V3 bei der HIIT-(F), MCT-(E) und CG-Gruppe(D) 42
Abbildung 13: Aktivität der Paraoxonase-1 im HDL 45
Abbildung 14: Aktivität der Paraoxonase-1 im Serum 46
Abbildung 15: TBARS-Konzentration im Serum [μmol/l] 48 / Background: Heart failure with preserved ejection fraction (HFpEF) is a complex disease. Due to its increasing hospitalization rates, HFpEF is gaining importance in the healthcare system. Despite the high mortality rate, there is no successful treatment procedure and no standard therapy has been established. Moreover, HFpEF makes up half of all heart failure cases, which underlines the usefulness of finding the effective therapy. One of the determining factors of HFpEF pathophysiology is endothelial dysfunction. The reduction of NO-bioavailability in endothelium and consequently endothelial dysfunction provides to LV stiffness and diastolic dysfunction of the heart. The exercise training triggers shear stress inside the blood vessels and results in HDL-mediated nitric oxide synthesis. In heart failure patients, the eNOS-increasing and vasodilative function of HDL can be impaired. However, a suitable training modality could work as endothelium-protective. Aims: The present doctoral thesis aims to investigate the influence of high-intensity interval training (peak HR 90-95%) and moderate-intensity continuous training (peak HR 60-70%) on the function of HDL by HFpEF patients. This study focuses on HDL-regulated eNOS function (function of endothelial nitric oxide synthesis), which is evaluated by phosphorylation of different aminoacids residues in the molecule. Furthermore, we determined the activity of PON-1 (paraoxonase-1), which speaks of the antioxidative role of HDL, as well the concentration of TBARS (thiobarbituric acid reactive substances), which indicate increased lipid peroxidation. Material and methods: To clarify the questions, the probands were randomized to three groups: 1) HIIT (high-intensity interval training, 2) MCT (moderate-intensity continuous training) and 3) CG (control group). Before, during, and after different training interventions the HDL was isolated from the blood serum of probands by ultracentrifugation. Further, the HDL isolates were incubated with human aortic endothelial cells. The HDL-induced eNOS phosphorylation at the activating position Ser1177 (which results in increased activity of eNOS) and deactivating Thr495 (which results in inhibition of eNOS activity) were assessed by western blotting. The activated phosphorylation of eNOS leads to increased NO production. Subsequently, to investigate how modifiable the endothelial-protective role of HDL is and to find the possible correlation, the antioxidative function of HDL was evaluated. The latter was assessed by measuring the activity of the associated enzyme PON-1 (paraoxonase-1), which protects the lipoproteins against oxidative modifications. The protective property of HDL might be among others modified by reactive oxygen species. The reduced activity of PON-1 can be associated with increased lipid peroxidation and therefrom resulting fatty acids radicals. In this study, the amount of fatty acids radicals was indicated by the concentration of TBARS (thiobarbituric acid-reactive substances). Results and observations: Exercise training can increase phosphorylation at Ser1177. Indeed, the first supervised part of HIIT resulted in a significant increase of HDL-induced eNOS-Phosphorylation at Ser1177. However, neither after HIIT nor after MCT any significant decline of eNOS phosphorylation at deactivated position Thr495 could be demonstrated. Regarding the eNOS phosphorylation after MCT and in CG, no significant changes were observed. Furthermore, significantly increased activity of PON-1 in serum as well as in HDL was observed after HIIT. But, neither in MCT nor in the control group any significant increase in PON-1 activity was observed. The increased concentration of TBARS was detected in all groups. After the interventions, no significant differences concerning the concentration of TBARS were demonstrated in any group. Conclusions: High-intensity interval training by HFpEF patients results in increased HDL- induced eNOS phosphorylation, which is accompanied by significantly increased activity of PON-1. Therefore, we can speculate that HIIT could enhance the endothelial function of HDL and would be in the future one of the possible effective treatment methods for HFpEF. Furthermore, this study derives that compliance in all groups was higher during the supervised training than during home-based intervention. Eventually, the usefulness of HIIT in clinical settings requires further investigation.:Inhaltsverzeichnis I
Tabellenverzeichnis IV
Abbildungsverzeichnis V
Abkürzungsverzeichnis VI
1 Einleitung 3
1.1 Die Herzinsuffizienz mit erhaltener Ejektionsfraktion 3
1.1.1 Definition der Herzinsuffizienz, Klinisches Bild, Einteilung nach der Pathophysiologie 3
1.1.2 Epidemiologie der HFpEF 3
1.1.3 Pathomechanismus der HFpEF 4
1.1.4 HFpEF vs. HFrEF 11
1.1.5 Diagnose der HFpEF 12
1.1.6 Behandlungsmöglichkeiten 12
1.2 Körperliches Training 14
1.2.1 Belastungsintoleranz bei HFpEF 14
1.2.2 Hochintensives Intervalltraining (HIIT) und Moderat-Intensives
Kontinuierliches Ausdauertraining (MCT) 15
1.3 High density lipoproteins 16
1.3.1 HDL bei Herzinsuffizienz 16
1.3.2 Funktionen und Bedeutung von HDL-Partikeln 17
1.4 Zielsetzung und Fragestellung 19
2 Material 20
2.1 Serum 20
2.2 Zellkulturmaterial 20
2.3 Kulturmedium 20
2.4 Chemikalien und Lösungen 20
2.5 Färbelösungen 20
2.6 Antikörper 20
2.7 Chemilumineszenz 20
2.8 Sonstige Materialien 21
2.9 Geräte 21
2.10 Software 21
3 Methoden 22
3.1 Studiendesign 22
3.2 Trainingsintervention 23
3.3 Isolation des HDL 24
3.3.1 Vorarbeiten 24
3.3.2 HDL Isolation 24
3.3.3 HDL Aufreinigung 25
3.4 Bestimmung der Proteinkonzentration nach der BCA-Methode 25
3.5 Stimulation der Zellen 26
3.6 Western Blot 26
3.7 Gelelektrophorese 27
3.8 Proteintransfer 28
3.9 Immundetektion 29
3.10 Chemilumineszenz 29
3.11 PON-1 Aktivität 30
3.12 TBARS 30
3.13 Statistische Analyse 30
4 Ergebnisse 31
4.1 Charakterisierung der Patienten 31
4.2 Patientendaten 32
4.3 Qualität des isolierten HDL 38
4.4 Die HDL-induzierte eNOS-Phosphorylierung 40
4.5 Aktivität der Paraoxonase 1 (PON-1) 44
4.6 TBARS-Konzentration im Serum 47
5 Diskussion 49
5.1 Hauptaussagen 49
5.1.1 Endotheliale Effekte des Trainings via HDL 50
5.1.2 Trainingsmodalitäten als potentielle Therapie 52
5.1.3 Denkbarer molekularer Mechanismus für die Regulation von
endothelialen Funktionen des HDL 55
5.1.4 Quantitative vs. qualitative Auswertung der HDL Funktion 57
5.1.5 Unterschiede bei der Einhaltung der verschiedenen
Trainingsprotokollen 59
5.1.6 Ausblick für zukünftige Forschungsarbeiten 61
5.1.7 Studienlimitationen 63
6 Zusammenfassung 65
7 Summary 67
8 Literaturverzeichnis 69
9 Anlage 1 88
10 Anlage 2 89
11 Curriculum vitae 90
12 Danksagung 91
Tabellenverzeichnis
Tabelle 1: Reagenzien zur Vorbereitung eines 8 % und 12 % SDS-Polyacrylamidgels 27
Tabelle 2: Die verwendeten Antikörper zur Detektion der Proteine 29
Tabelle 3: Patientendaten zu Beginn der Studie -V1 32
Tabelle 4: Patientendaten zu den Zeitpunkten V1, V2, V3 33
Tabelle 5: Medikation 34
Tabelle 6: Lipidprofil 36
Tabelle 7: Ergospirometrie 37
Abbildungsverzeichnis
Abbildung 1: Pathomechanismus der myokardialen Dysfunktion (übernommen aus Paulus & Tschöpe, 2013) 6
Abbildung 2: Entstehung der myokardialen Dysfunktion durch Veränderungen innerhalb extrazellulärer Matrix und Kardiomyozyten bei Übergewicht und Diabetes mellitus (DM) 7
Abbildung 3: Komorbiditäten bei HFpEF als Trigger für diastolische Dysfunktion 9
Abbildung 4: NO Synthese als Antwort auf Shear Stress 10
Abbildung 5: Schematische Darstellung, wie ein körperliches Training das Krankheitsfortschreiten sowie HDL-induzierte NO-Produktion beeinflusst (übernommen aus Adams et al., 2013) 18
Abbildung 6: Studiendesign 22
Abbildung 7: Trainingsprotokoll, MCT-Moderat-Intensives Kontinuierliches Ausdauertraining, HIIT- Hochintensives Intervalltraining, HRpeak- maximale Herzfrequenz (übernommen aus Suchy et al., 2014) 23
Abbildung 8: Positionieren der Kanülenspitze und Entnahme des HDL 25
Abbildung 9: SDS-Page nach Färbung mit Coomassie-Blau zum Nachweis von HDL mit Größenmarker, BSA-Standard und den HDL-Proben (10 μg, 20 μg und 30 μg des Proteins) 38
Abbildung 10: Die Immundetektion der gebundenen Antikörper Apo-Protein A 1 durch eine Chemilumineszenz-Reaktion mit Größenmarker (M) zum Nachweis von HDL. Apo-AI, das Hauptprotein des HDL, mit einer Molekülgröße von 28 kDa wurde in 1,95 μg, 0,99 μg, 0,5 μg und 0,099 μg des HDL-Isolates identifiziert 39
Abbildung 11: Die eNOS-Phosphorylierung an Ser1177 zu den Zeitpunkten V1, V2, V3 bei der HIIT-(C), MCT-(B) und CG-Gruppe-(A) 40
Abbildung 12: Die eNOS-Phosphorylierung an Thr495 zu den Zeitpunkten V1, V2, V3 bei der HIIT-(F), MCT-(E) und CG-Gruppe(D) 42
Abbildung 13: Aktivität der Paraoxonase-1 im HDL 45
Abbildung 14: Aktivität der Paraoxonase-1 im Serum 46
Abbildung 15: TBARS-Konzentration im Serum [μmol/l] 48
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O papel da enzima Na+,K+-ATPase no déficit cognitivo e no efeito profilático induzido pelo exercício físico após o Traumatismo Crânio-Encefálico / The role of Na+,K+-ATPase enzyme on cognitive deficit and in the prophylactic effect induced by exercise after Traumatic Brain InjuryLima, Frederico Diniz 17 September 2009 (has links)
Traumatic Brain Injury (TBI) is the major cause of death or cognitive deficits in industrialized countries. Although studies have indicate that the oxidative stress and functional deficits after TBI are connected events, the mechanisms that outline the development of these cognitive deficits are, still, limited. In this context, we
investigated the involvement of oxidative stress markers (thiobarbituric acid reactive species; TBARS and protein carbonylation) and the Na+,K+-ATPase enzyme activity
on the spatial learning after one and three months from a fluid percussion injury (FPI) in rats. The results revealed that FPI increase the latency of escape and the number of the errors on the Barnes Maze Test one and three months after FPI. We also found an increase of TBARS and protein carbonylation in parietal cortex after one and three months FPI. In addition, statistical analysis revealed a decrease of the Na+,K+- ATPase enzyme activity in the parietal cortex after FPI (time-dependent). These
results suggest that cognitive impairment following FPI may result, at least in part, from increase of two oxidative stress markers, protein carbonylation and TBARS that occurs concomitantly to a decrease in Na+,K+-ATPase activity. Physical exercise, despite the involvement on the generation of the reactive oxygen species (ROS), is
used on the rehabilitation of TBI. However, although the favorable effects of physical exercise on traumatic brain injury (TBI) patients is well known, the specific
mechanisms involved in this protection after TBI has been limited. Thus, we investigated whether physical training protects against oxidative damage (measured by protein carbonylation and TBARS) and neurochemical alterations represented by immunodetection of alpha subunit and activity of Na+,K+-ATPase after FPI in cerebral cortex of rats. The results revealed that physical training protected against oxidative damage induced by FPI. In addition, physical training was effective against Na+,K+-
ATPase enzyme activity inhibition and α subunit level decrease after FPI. The Pearson correlation showed that the decrease of the catalytical levels of the Na+,K+-
ATPase enzyme α subunit is related with the increasing on oxidative stress markers. Moreover, the physical activity-related protection against free radicals induced by FPI
links with maintenance of α subunit immunocontent. These results suggest that the effective protection stimulated by physical exercise on the neuronal damage induced
by TBI has connection with the protection of the specific targets from the free radicals action, like Na+,K+-ATPase enzyme. / O Traumatismo crânio-encefálico (TCE) é uma das maiores causas de morte ou déficits cognitivos nos países industrializados. Apesar de os estudos indicarem que o
estresse oxidativo e os déficits funcionais que ocorrem após TCE serem eventos interrelacionados, os mecanismos que delineiam o desenvolvimento destes déficits cognitivos
são, ainda, limitados. Neste contexto nós investigamos o envolvimento de marcadores de estresse oxidativo (espécies reativas ao ácido tiobarbitúrico; TBARS e carbonilação
protéica) e a atividade da enzima Na+,K+-ATPase no aprendizado espacial um e três meses após um dano de percussão por fluído (FPI) em ratos. Os resultados revelaram que o FPI aumentou o tempo de latência e o número de erros no teste do labirinto de Barnes em um e três meses após FPI. Também encontramos aumento no conteúdo de TBARS e proteína carbonil no córtex parietal em um e três meses após FPI. Além disso, a análise estatística revelou uma diminuição na atividade da enzima Na+,K+-ATPase no córtex cerebral após FPI tempo dependente, sugerindo que o déficit cognitivo induzido pelo FPI se deva pela perda de funcionabilidade de enzimas presentes na células como Na+,K+-ATPase. Perda esta induzida pelo aumento na geração de radicais livres após TCE. Apesar de estar envolvido no aumento da produção de espécies reativas ao Oxigênio (ERO), exercício físico tem sido
utilizado na reabilitação de após TCE. Por outro lado, ainda são escassos na literatura estudos que evidenciam a especificidade dos mecanismos envolvidos na proteção induzida pelo exercício físico após TCE. Desta forma, investigamos se o treinamento físico protege contra o dano oxidativo bem como das alterações neuroquímicas representadas pela imunodetecção da subunidade α e da atividade da enzima Na+,K+-ATPase no córtex cerebral de ratos. Os resultados revelaram que o treinamento físico protegeu contra o dano oxidativo induzido por FPI. Além disso, o treinamento físico foi efetivo contra a inibição da
enzima Na+,K+-ATPase e a diminuição dos níveis da sua subunidade α após FPI. A correlação de Pearson revelou que a diminuição dos níveis catalíticos da subunidade α da
enzima Na+,K+-ATPase se correlaciona com o aumento dos marcadores de estresse oxidativo. Além disso, a proteção exercida pela atividade física contra os radicais livres
induzidos por FPI tem relação com a manutenção do imunoconteúdo da subunidade α. A partir destes achados, sugere-se que a efetiva proteção exercida pelo exercício físico no dano neuronal causado induzido pelo TCE se deva pela proteção de alvos específicos a ação de radicais livres, como a enzima Na+, K+-ATPase.
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