A utilização da eletroestimulação muscular é uma estratégia utilizada para investigação da fadiga neuromuscular. Entretanto, esta técnica é pouco aplicada na musculatura extensora do cotovelo em função de limitações biomecâncias (atuação de músculos sinergistas), neurais (influência de reflexo neural) e fisiológicas (facilidade em atingir estados de potencialização pós-ativação e fadiga). Portanto, o objetivo deste estudo foi padronizar procedimentos para avaliação neuromuscular da musculatura extensora do cotovelo. Para isso, foram realizados três estudos que envolveram a adequação da técnica de eletroestimulação e um \"estudo principal\" para validar um novo protocolo de avaliação neuromuscular. Após resultados positivos em estudos complementares, a presente dissertação propôs a validação de um protocolo para análise neuromuscular com contrações submáximas para a musculatura extensora do cotovelo. Vinte participantes com idade de 25,1 ± 6,8 anos foram submetidos, em dias diferentes, a dois esforços, um a fim de promover fadiga central e fadiga periférica respectivamente. Antes e após os esforços envolvendo fadiga (periférica ou central) foram realizadas avaliações de fadiga neuromuscular para musculatura extensora do joelho (protocolo já validado) e musculatura extensora do cotovelo (protocolo proposto). Verificou-se que: (i.) o modelo de análise proposto não promove nenhum tipo de fadiga ou potencialização pós-ativação; (ii) a resposta de um pulso controle no início e no fim do modelo de análise proposto promovem respostas diferentes; (iii) o modelo proposto foi capaz de identificar redução da resposta neuromuscular; (iv) o esforço envolvendo fadiga central não promoveu fadiga central significante; (v) a utilização de um modelo matemático para investigação de fadiga neuromuscular (coeficientes resultantes de uma regressão linear) com os valores de estimulação em contrações submáximas foi sensível a fadiga mas não foi capaz de discriminar fadiga central e periférica. É possível concluir que o modelo de análise proposto para a musculatura extensora do cotovelo apresentou sensibilidade para verificar a fadiga neuromuscular. Entretanto, este não foi capaz de discriminar fadiga central e periférica. A utilização da regressão linear parece ser uma boa estratégia para diferenciar os dois modelos de fadiga e deve ser estudada com mais profundidade futuramente. / Muscular electrostimulation is a strategy used to investigate neuromuscular fatigue. However, this technique is poorly applied in the elbow extensor muscles due to the limitations of biomechanics (acting of synergistic muscles), neural (influence of neural reflex) and physiological (easiness to achieve post-activation and fatigue states of potentiation). Therefore, the purpose of this study was to standardize a neuromuscular evaluation strategy for the elbow extensor musculature. Thus, three complementary studies aimed at adjusting the electrostimulation technique and one \"main study\" to validate a new neuromuscular assessment protocol were realized. After positive results in complementary studies, the present dissertation proposed a protocol validation for neuromuscular analysis with submaximal contractions on the elbow extensor musculature. Twenty participants with 25.1 ± 6.8 years old performed, on different days, two efforts, one to promote central fatigue and peripheral fatigue respectively. Before and after the exercise involving fatigue (peripheral or central) there were realized neuromuscular fatigue evaluations for knee extensor muscles (protocol already validated) and elbow extensor musculature (protocol). The results indicate that: (i) the proposed analysis model does not promote any type of fatigue or post-activation potentiation; (ii) the response of a control pulse at the beginning and end of the proposed analysis model promotes different responses; (iii) the proposed model was able to identify reduction of neuromuscular response; (iv) the effort involving central fatigue did not promote significant central fatigue; (v) the use of a mathematical model to investigate neuromuscular fatigue (coefficients resulting from a linear regression) with the values of stimulation in submaximal contractions was sensitive after fatigue but was not able to discriminate central and peripheral fatigue. It is possible to conclude that the proposed analysis model for the elbow extensor musculature presented sensitivity to verify the neuromuscular fatigue. However, it was not able to discriminate central and peripheral fatigue. The use of linear regression seems to be a good strategy to differentiate the two models of fatigue and should be studied in more depth in the future.

De så kallade donationer som erhålls via streaming på Twitch är ett område i svensk skatterätt där oklarhet fortfarande råder. Lagstiftning finns gällande hur gåvor skall behandlas skattemässigt, dock är vägledning till just dessa så kallade donationer som erhålls vid streaming begränsad. Den vägledning som finns i rättskällor är ett förhandsavgörande från Skatterättsnämnden, DNR 85-15/D. Där fastslog nämnden att donationer som erhålls vid streaming inte utgör benefika förvärv enligt 8:2 inkomstskattelagen (1999:1229) (IL) och att de utgör en skattepliktig inkomst. Det är just det som undersökts i denna uppsats. Skall så kallade donationer som erhålls från streaming via Twitch ses som en skattepliktig inkomst och, om så är fallet, hur bör de beskattas enligt svensk lag? Uppsatsen beskriver vad Twitch är och dess ekonomiska aspekter. Efter det sker en genomgång av vad som utgör skattepliktiga inkomster. Därefter sker även en genomgång av vad som utgör skattefria inkomster samt gåvor enligt svensk rätt. Slutligen analyseras all den fakta som har samlats för att besvara problemformuleringen i denna uppsats. I uppsatsen konstateras att de så kallade donationerna erhållna via streaming inte utgör skattefria inkomster enligt 8:2 IL, på grund av att överföringarna utgör ersättning för streamingen. Sedan undersöks det om överföringarna kan utgöra skattepliktiga inkomster enligt IL, då det inte utgör skattefria gåvor. Resultatet av analysen visar att överföringarna kan utgöra skattepliktiga inkomster i inkomstslagen näringsverksamhet och tjänst. Vilket inkomstslag de så kallade donationerna skall hänföras till beror på hur streamingen bedrivs.

Orientador: Fausto Berzin / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-15T03:24:24Z (GMT). No. of bitstreams: 1 Sakabe_FabianaForti_D.pdf: 2084817 bytes, checksum: b0340d79b05862a9990b4042a871cf3b (MD5) Previous issue date: 2010 / Resumo: Tem sido demonstrado que os sinais eletromiográficos e a histomorfometria diferem ao longo do ventre muscular e entre os gêneros, porém a relação entre esses parâmetros não está bem estabelecida. O objetivo deste trabalho foi avaliar a eletromiografia (EMG) em diferentes intensidades de contração (IC) isométrica voluntária (10 a 100%) e posicionamentos de eletrodos (PE) sobre o músculo vasto lateral proximal (VLP) e distal (VLD), assim como a histomorfometria em ambos os gêneros. Participaram 11 mulheres e 7 homens (22,0+2,1 e 23,3+2,5 anos, respectivamente), sem lesões nos membros inferiores. Inicialmente foi obtida uma linha de base para determinação da força máxima de extensão da perna (célula de carga MM-100 (KRATOS®)). Para a coleta da EMG foi utilizado o módulo de aquisição EMG-1000 da Lynx® (16 bits de resolução, filtro passa-banda de 20-1000 Hz e freqüência de amostragem 2000 Hz). Dois eletrodos de superfície ativos simples diferencial (Lynx®, ganho 20x) com distância intereletrodo de 10mm foram posicionados sobre o VLP e VLD. O eletrodo de referência foi fixado à tuberosidade da tíbia. O sinal foi coletado simultaneamente nos eletrodos e na célula de carga em diferentes IC por 5 segundos, repetida por 3 vezes e com intervalo de 1 minuto. A EMG foi processada no software Matlab® 6.5.1, sendo determinados o RMS e a freqüência mediana (FM). Após a EMG, foi realizada a biópsia do VLP e VLD com agulha de Bergström. Os cortes (12um) foram realizados em criostato (MICRON HM 505E a -25°C). Os principais tipos de fibras (TF) musculares (I, IIA e IID) foram delineados pela técnica dxa mATPase e a área das fibras (um2) foi analisada no software Image Manager (Leica Microsystems®). Foram utilizados os testes ANOVA com post-hoc de Tukey, o teste T de Student pareado e não pareado e a correlação de Pearson. Com o aumento da IC (10100% CIVM) houve elevação significativa do RMS (em ambos os PE e gêneros). Para a FM, de maneira geral, não houve variação com o aumento da IC (nos PE e gêneros). A força e o RMS bruto foram maiores nos homens em relação às mulheres para os dois eletrodos. A FM não diferiu entre os gêneros. Para a distribuição (%) dos TF, não houve variação tanto nos gêneros quanto nos PE. Entretanto, a área dos diferentes TF foi maior nos homens do que nas mulheres e de maneira geral, não houve variação entre os PE. Verificou-se correlação positiva entre força x área I e área II, RMS x área I e área II. Não houve correlação entre área das fibras e FM, nem entre força e FM. De acordo com os resultados obtidos, pode-se verificar que o percentual de fibras não variou entre os PE e gêneros, porém os homens apresentaram os TF, a força e o RMS maiores do que as mulheres. A FM não diferiu entre os gêneros. Deste modo, a área das fibras musculares influenciou o RMS e a força do músculo vasto lateral, sendo a diferença entre os gêneros provavelmente devido a essa variável. / Abstract: It has been shown that electromyography signal (EMGs) and histomorphometry are different along the muscle belly and between genders, although the relationship between these parameters is not clear. The aim of this study was to evaluate the EMGs in different intensities of volunteer isometric muscle contraction (10-100%) and electrodes positions over the belly of vastus lateralis muscle [proximal (VLP) and distal (VLD) portions], as well as the histomorphometry for both genders. Eleven women (22,0 ± 2,1 years) and seven men (23,3 ± 2,5 years), took part in the study. All subjects had no lesions in lower limbs. At first a baseline was made to determine the maximum force of leg extension [MM-100 load cell (KRATOS®)]. For EMG recording an EMG-1000 acquisition module (Lynx®) was used (16 bits of resolution, band-pass filter of 201000Hz and sampling frequency of 2000Hz). Two simple differential surface active electrodes (Lynx®, 20 times gain) separated by 10mm were placed over VLP and VLD muscles bellies. Reference electrode was attached to the anterior tibial tuberosity of the evaluated leg. EMGs was recorded simultaneously in both electrodes in different intensities of contraction for 5 seconds, repeated for 3 times and with one-minute intervals among contractions. Load cell signals were also recorded. EMGs were analyzed in Matlab® 6.5.1 software and RMS and median frequency (MF) values were obtained. After EMGs recordings, the muscle biopsies of VLP and VLD were performed using a Bergström needle. Histological sections (12um) were performed in cryostat (MICRON HM 505E at -25°C). The main types of muscle fibers (I, IIA and IID) were identified by mATPase technique and fibers' area (um2) was analyzed in Image Manager software (Leica Microsystems®). ANOVA test and Tukey post-hoc test, paired and non-paired Student t test, paired Student t test and Pearson test (correlations). A significant elevation of RMS was observed with the rise of contraction intensity (10-100% maximum voluntary isometric contraction - MVIC) for both genders and electrodes positions. In general, there were no variations in MF with the rise of contraction intensity (for both genders and electrodes positions). Force and absolute RMS values were higher in men compared to women for both electrodes. MF was not different between genders. For fiber types distribution (%) there was no difference between genders and between electrodes positions. However, male's fibers' areas were greater than women's and, as a general matter, there was no difference between the electrodes. A positive correlation was observed between force x type I and II fiber areas, RMS x type I and II fiber areas. There was no significant correlation between fibers' area and MF and between force and MF. According to our results the fiber type's distribution was not different between genders and electrodes; nevertheless, higher RMS and force values, as well as greater fibers' areas, were found in male group. MF was not different between genders. Finally, muscle fibers' area did influence RMS and force of vastus lateralis muscle and we suggest that this could be the reason of the observed differences between genders. / Doutorado / Anatomia / Doutor em Biologia Buco-Dental

Storskaliga öppna nätbaserade kurser, MOOCar, med självstyrd studietakt skapar en lärandesituation där kursdeltagaren står i centrum och aktivt kan påverka sin egen studieprocess. Ett problem som kursdeltagare upplever är att de får begränsat stöd och hjälp av lärare eller andra kursdeltagare. Möjligheten till social interaktion är avgörande för främjandet av lärandeprocessen i nätbaserade utbildningar visar tidigare forskning. Studien ämnar därför att utforska om direktuppspelning (livestreaming) kan användas som ett pedagogiskt verktyg för att främja den sociala interaktionen mellan kursdeltagare samt mellan kursdeltagare och lärare i MOOCar med självstyrd studietakt. Utifrån en systematisk litteraturstudie utformades ett moment med direktuppspelning via Twitch som testades i praktiken i en MOOC som gavs av Kungliga Tekniska högskolan (KTH). Vi utvärderade sedan momentet genom en enkät till kursdeltagarna och intervju med läraren. En intervju genomfördes också med ytterligare en lärare på KTH som tidigare genomfört ett antal kursmoment där direktuppspelning använts. MOOCar karakteriseras av att de skall vara tillgängliga för alla, därav valdes användarvänlig och kostnadsfri plattform och programvara. På grund av att en direktuppspelning inom MOOCar ska kunna stödja ett stort deltagarantal gavs kursdeltagarna enbart möjlighet att kommunicera via chatt medan läraren kunde kommunicera via såväl chatt som ljud och video. Studien visade att såväl kursdeltagare som lärarna upplevde direktuppspelning som ett givande komplement till de kommunikationsmöjligheter som redan ges i MOOCar med självstyrd studietakt men att läraren bär ett stort ansvar i att möjliggöra de sociala interaktionerna som direktuppspelning ger möjlighet till. Direktuppspelning visades även ge en personlig kontakt som kan hjälpa minimera känslan av att vara isolerad. Baserat på studien utformades en manual för att underlätta implementeringen för lärare i framtida kurser. / Self-paced massive open online courses, MOOCs, provide individual learners with the opportunity to take control of their learning. However, MOOCs require learners to be able to fulfil the course with limited help from teachers and peers - something that is highlighted as an essential factor to enhance the learning experience in distance education. Therefore this study aims to explore the use of live streaming as a pedagogic tool in self-paced MOOCs to enhance communication between learners and between learners and the teacher. Based on a systematic literature study, a live streaming event was designed and conducted via Twitch in a self-paced MOOC given by KTH Royal Institute of Technology. To evaluate the event surveys were handed out to the learners and the teacher was interviewd. An interview was also conducted with another teacher at KTH who had experience with planning and executing live streaming events. Since MOOCs are characterized as being accessible to everyone a user-friendly and free platform plus software were selected. Due to the fact that a live streaming event in MOOCs must be able to support a large number of learners, we chose to allow learners to communicate only via chat while the teacher was able to communicate via chat, audio and video. The study showed that both the learners and the teacher perceived this moment as a rewarding complement to the opportunities for communication already provided in self-paced MOOCs but that the teacher has a major responsibility in enabling the interactions that live streaming allows. The teacher also felt that live streaming provided a personal contact and could therefore be a means to minimize the sense of isolation that is often associated with self-paced MOOCs. The study further designed a manual to facilitate implementation in future courses.

Metabolic Syndrome Insulin Resistance Is Associated with Discordant Distribution of GLUT4 and the Insulin Receptor in Fast-Twitch and Slow-Twitch Muscle Fiber Types We have previously shown that We have previously shown that strength training alone improved insulin responsiveness in sedentary controls but not in metabolic syndrome subjects. Immunoblots of metabolic syndrome subjects[apos] muscle homogenates showed training-related increases in GLUT4 and mitochondrial enzymes was half that seen in the controls. To determine if this was due to changes primarily in fast-twitch fibers (strength fibers), we performed immunohistochemical (IHC) studies on muscle sections from these subjects to quantify fiber-specific changes in GLUT4, phospho-AMPK, phospho-mTOR, ATP synthase, and the insulin receptor. Signal intensity in confocal microscopic images was digitally quantified and the amount in each fiber type was adjusted by the fiber composition and the average size of each fiber type. Fiber type was classified using monoclonal antibodies against slow-twitch (type 1 fibers) and fast-twitch (type 2a and 2b fibers) myosin heavy chains. At baseline, both groups had slightly more insulin receptor in slow-twitch fibers, and most of the ATP synthase (mitochondrial marker) was in fast-twitch fibers. In controls, 55% of GLUT4 was in slow-twitch fibers, whereas metabolic syndrome subjects had only 33% of their GLUT4 in slow-twitch fibers. The IHC data showed modest increases in GLUT4 (9-25%), and substantial increases of ATP synthase (55-95%), and insulin receptors (44-104%) in both fiber types in both groups. Training-related increases were seen in phospho-AMPK (25% in slow-twitch, 15% in fast-twitch) only in the control subjects but no change in phospho-mTOR in either subject group. At baseline, metabolic syndrome subjects[apos] muscle had 56% of insulin receptors expressed in slow-twitch fibers, but only 33% of the GLUT4 was in these fibers. Thus, the untrained muscle composition of the metabolic syndrome subjects exhibited a mismatch between insulin receptors and GLUT4 in their fiber-specific distributions. This mismatch may contribute to the insulin resistance seen in the metabolic syndrome and may be involved in the diminished insulin sensitivity response to strength training in these subjects.

Att försörja sig som streamingpersonlighet på Internet är något som vuxit explosionsartatpå senare tid. Detta leder också till att man blir ett tydligt mål för attacker. Detta arbetehar belyst den problematik som DDoS-attacker skapar när de riktas mot hemanvändare.Olika lösningar på detta problem gås igenom och analyseras. Tester har utförts för attbelysa enkelheten i attacken samt hur den påverkar hemanvändares hårdvara. Testernahar resulterat i att VPN är en kompetent lösning men innehåller även nackdelar. / To make a living as streaming personality on the Internet is something that has grownexponentially in recent times. This also leads to one becoming a clear target for attacks.This work has highlighted the problems that DDoS-attacks create when they are aimedat home users. Various solutions to this problem are reviewed and analyzed. Tests havebeen conducted to illustrate the simplicity of the attack and how it affects home usershardware. The result of the tests has shown that VPN is a competent solution but also hasits disadvantages.

En undersökning av hur online-exponering påverkar kreativa processer. Genom att arbeta i en öppen live-stream har Jerker Österlind inför publik skapat en EP under 30 Twitch-sändningar. Denna text utforskar effekterna av att bjuda in åskådare till vad som brukar ske bakom stängda dörrar, hur det musikaliska resultatet påverkas, hur arbetsprocessen förändras och funderingar om hur livestreaming kan ge nya möjligheter för marknadsföring och karriär. https://open.spotify.com/album/18uGeyxD00Ssayi1exCCJo?si=qbFel0xUTaeF4rXiwlR2GQ / An inquiry about the impacts of online exposure on creative processes. By working in a public livestream, Jerker Österlind has made an EP in front of an audience during 30 Twitch broadcasts. This text explores the effects of inviting an audience to things that usually take place behind closed doors, how the musical result is affected, how work methods are changed and how livestreaming can open up new opportunities for marketing and careers.  https://open.spotify.com/album/18uGeyxD00Ssayi1exCCJo?si=qbFel0xUTaeF4rXiwlR2GQ / <p>Arbetet presenteras i form av en EP med fem spår. Alla instrument är spelade av J. Österlind.</p><p>Musiken kan höras här:</p><p>https://open.spotify.com/album/18uGeyxD00Ssayi1exCCJo?si=qbFel0xUTaeF4rXiwlR2GQ</p><p>och här:</p><p>https://mudbody.bandcamp.com/album/underachieve-ep</p>

Introduction: Acute enhancements of muscle contractile properties and performance subsequent to a maximal or near maximal conditioning contraction are often termed post activation potentiation (PAP). Although still controversial, PAP is commonly linked to enhancements in the myosin regulatory light chain phosphorylation, leading to improvements in the excitation–contraction coupling. The PAP seen after a conditioning task often coexists with fatigue and is known to depend on strength level, muscle fiber type and age. Less is known about how factors such as static and dynamic changes in muscle length affect PAP, and on the relative contribution of contractile and tensile components to PAP. Aim: To enhance our understanding of how, and under what conditions, a single maximal isometric contraction affects plantar flexor muscle contractile performance, and other muscle tendon properties, in power athletes. Methods: Supramaximal twitches were evoked via electrical stimulation of the tibial nerve of athletes before and on several occasions after a 6-second maximal voluntary isometric contraction (6-s MVIC) in both static muscle, and during passive muscle lengthening and shorting at different angular velocities. Several contractile variables were measured from the twitches. The effects of a 6-s MVIC on Achilles tendon stiffness was calculated from torque and ultrasonography based measurements of tendon length at two submaximal contraction intensities. Overall stiffness index was calculated by analyzing the passive lengthening torque/angle curve.Results: A single MVIC enhanced muscle contractile properties and electromechanical delay for up to 5 minutes. Plantar flexor twitch variables such as peak twitch, rate of torque development and rate of torque relaxation were enhanced during shortening compared to lengthening muscle actions, and in an extended as compared to a flexed knee position. Achilles tendon stiffness and overall stiffness index were not significantly modulated by a single 6-s MVIC. Conclusion: The results of this thesis imply that functional enhancements from a 6-s conditioning MVIC would mainly come from improvements in contractile rather than tensile components. Stiffness changes should be monitored in future PAP-related studies since they may still occur after more extensive conditioning protocols than the current one. Improvements in contractile components subserving muscle strength after a conditioning MVIC suggests that enhancements in muscle power after a conditioning task should be greatest in fast concentric muscle actions, though still present in muscle lengthening. Conditioning should be performed in a position where full activation is easy to achieve and tailored to mach an athlete or group of athlete’s current status and characteristics, maximizing performance in a specific sport event. / <p>The project recived financial support from the Swedish National Centre for Research in Sports (CIF). Paulo Gago also wishes to thank the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. Grant (SFRH/BD/103572/2014).</p><p>New version 2015-01-25 updates the previous one by correcting the errors described in the correction list file (errata).</p> / Doctoral project: Post activation potentiation - Modulating factors and mechanisms for muscle performance.

Multiple resources currently exist that provide tips, tricks, and hints on gaining greater success, or increasing one’s chances for success, in the field of live content creation. However, these resources often lack depth, detail, large sample size, or significant research on the topic. The purpose of this thesis is twofold: to aggregate and optimize the very best methods for live content creators to employ as they begin a streaming career, and how best to implement these methodologies for maximum success in the current streaming market. Through analysis of a set of semi-structured interviews, popular literature, and existing, ancillary research, repeating patterns will be identified to be used as the basis for a structured plan that achieves the stated objectives. Further research will serve to reinforce as well as optimize the common methodologies identified within the interview corpus.

The group II metabotropic glutamate receptors are known for their involvement in various psychiatric disorders. The mGluR2 in particular is linked with etiology of schizophrenia especially in the context of crosstalk with 5-HT2A. Thus, the mGluR2 has attracted attentions for its potential therapeutic applications. Despite numerous physiological evidences on the actions of mGluR2, its mechanism is still unclear to this day. It is partially due to the lack of understanding in characteristics of mGluR2 homodimer which is its functionally active form. Therefore, the characterization of dimeric interaction serves as a foundation to advanced understanding of the role of mGluR2. On that note, the role of the conserved cysteine residue (C121) in the ligand binding domain of mGluR2 has been evaluated in this study as they are known to play a critical part in homodimer formation. Collectively, C121 has been shown to affect the dimerization, subcellular localization, and pharmacokinetics of mGluR2. Lastly, the effect of mGluR2 on mouse behavior was examined in a partial effort to elucidate its role in crosstalk with 5-HT2A.