Orientador: Peterson Bueno de Moraes / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Tecnologia / Made available in DSpace on 2018-08-23T06:55:03Z (GMT). No. of bitstreams: 1 Oliveira_CleliaAparecidadaSilva_M.pdf: 1434373 bytes, checksum: 2411a1ce9b13fbe4e8f7d778c6dfd3ea (MD5) Previous issue date: 2013 / Resumo: A indústria têxtil gera elevados volumes de efluentes com alta carga orgânica e compostos recalcitrantes, os quais são tratados por sistemas baseados em processos físicos, químicos e biológicos convencionais. Entretanto, o caráter não destrutivo dos tratamentos convencionais representa um sério problema no setor. Nos últimos 20 anos, os Processos Oxidativos Avançados (POA) têm estado em evidência devido à sua capacidade em degradar inúmeros compostos orgânicos contidos em águas e efluentes. Uma grande quantidade de trabalhos utilizando luz UV a partir de lâmpadas de vapor de mercúrio tem resultado em elevada eficiência de degradação de substratos recalcitrantes incluindo efluentes têxteis; entretanto, demandam elevado consumo de energia elétrica, encarecendo o tratamento. Em contrapartida, o surgimento de Diodos Emissores de Luz Ultravioleta (LED UV) abre novas fronteiras de aplicação no campo de tratamento de águas residuárias, quanto a custo, operacionalidade e tamanho dos sistemas. Nesse trabalho estudou-se a degradação de um efluente têxtil simulado contendo o corante Remazol Azul Brilhante (C.I. Reactive Blue 19) através de processos eletroquímicos e fotoeletroquímicos que utilizam LED UV, utilizando-se dois reatores: um operando em batelada contendo o fotocatalisador TiO2 e o outro, em fluxo, contendo um cátodo (tela cilíndrica de aço-inoxidável), um tubo de quartzo contendo os LED UV e o Anodo Dimensionalmente Estável (ADE 70%TiO2/30%RuO2). Os resultados demonstraram que, no reator de bancada, a eficiência de remoção de cor foi de 100% para concentração inicial de 50 mg L-1 do corante, em 24 horas de tratamento. No reator em fluxo, utilizando Na2SO4 como eletrólito, o processo eletrolítico resultou em eficiência de 65%; o fotoeletrocatalítico, em 68%, operando a 750 L h-1 e em 57,3 mA cm-2. Quando foi utilizado o eletrólito NaCl, obteve-se remoção de 100% da cor em 5 minutos de tratamento a 750 L h-1, independente da concentração inicial do corante utilizada (50 mg L-1 ou 100 mg L-1), da concentração do eletrólito (0,05 M ou 0,1 M), da densidade de corrente (14,3 mA cm-2 , 28,7 mA cm-2 ou 57,3 mA cm-2) e do processo utilizado / Abstract: The textile industry generates large amount of wastewater containing significant organic load and recalcitrant compounds, which in most cases are treated by conventional systems involving physical, chemical and biological processes, the latter represented mainly by activated-sludge treatment. However, the non-destructive profile of conventional treatments is a serious problem for textile-based industry. Over the past 20 years, the study of Advanced Oxidation Processes (AOP) has been carried out due to its high capacity degradation of numerous organic pollutants contained in waters and wastewaters. Research using UV light from mercury vapor lamps usually has resulted in high efficiency degradation of recalcitrant substrates including textile effluents but requires high electrical power consumption besides other drawbacks. In contrast, the emergence of Ultraviolet Light Emitting Diodes (UV LED) opens new perspectives for application on wastewater treatment, concerning efficiency, footprint and costs of the systems. In this work we studied the degradation of a simulated wastewater containing a textile dye, Remazol Brilliant Blue (C.I. Reactive Blue 19) through electrochemical and photoelectrochemical processes using UV LED as ultraviolet radiation source. The experimental apparatus consisted of two systems: the first, a bench-scale reactor containing TiO2 photocatalyst (P25 DEGUSSA) in solution, and another pilot-scale system operated in batch recirculation mode composed of an tubular stainless-steel screen cathode, a quartz tube containing the UV LED and a oxide-coated titanium anode (DSA©30%TiO2/70%RuO2). The results showed total decolorization of a solution containing 50 mg L-1 of RB in 24-hour treatment in the bench-scale reactor. Tests on flow reactor using Na2SO4 as supporting electrolyte resulted in 65% of color removal using electrolytic process and 68% for photoelectrocatalytic process operating at 750 L h-1 and 57.3 mA cm-2. In experiments using the electrolyte NaCl it was obtained 100% in the color degradation within 5 minutes of treatment at 750 L h-1, regardless of the: initial concentration of dye used (50 mg L-1; 100 mg L-1), concentration of the electrolyte (0.05 M; 0.1 M) and current density value (14.3; 28.7; 57.3 mA cm-2) / Mestrado / Tecnologia e Inovação / Mestra em Tecnologia

Résumé : Depuis l'interdiction en 2013 des tests sur les animaux par le Règlement cosmétique n°1223/2009, différentes méthodes in vitro ont été développées. Toutefois, selon un consensus scientifique, aucune méthode ne peut couvrir à elle seule l’ensemble des événements clés (KE) définis pour la sensibilisation cutanée.Après un état de l’art des méthodes alternatives relatives à la sensibilisation cutanée, nous avons sélectionné et comparé 3 tests pour ensuite déterminer la meilleure stratégie à suivre. Dans le but de proposer un nouveau test intégré, nous avons adressé l’ensemble des KEs au sein d’un même type cellulaire. La cellule dendritique (DC) jouant un rôle clé dans le développement de la dermatite de contact allergique (DCA), notre choix s’est porté sur la lignée humaine pro-monocytaire THP-1. Nous avons étudié comme événements initiaux (KE1) les formes réactives à l’oxygène (FRO) et le glutathion (GSH), la voie Nrf2-Keap1 (voie centrale de détoxication) et l’expression génique pour le KE2, ainsi que les modifications phénotypiques pour le KE3.Nous avons montré que les allergisants forts induisent une production précoce des FRO associée à une réduction du GSH. Ils activent également la voie Nrf2-Keap1 et induisent l’expression des marqueurs de surface cellulaire CD54 et CD86, ainsi qu’une production de cytokines spécifiques (IL-8, IL-18,...).Pour conclure, ce travail a permis de proposer un test intégrant l’ensemble des mesures biologiques comme différents KE au sein d’un même type cellulaire. / Abstract : Since the animal testing ban in 2013 by Cosmetics Regulation n°1223/2009, various in vitro methods have been developed. However, according to a scientific consensus, no single method can stand-alone to cover the different key events (KE) defined for skin sensitization.After a state of the art of alternative methods relating to skin sensitization, we selected and compared 3 tests to determine the best strategy to follow. In order to propose a new integrated test, we wanted to address all KE within the same cell line. Because dendritic cell (DC) plays a key role in the development of allergic contact dermatitis (ACD), we have chosen the pro-monocytic human line THP-1. We have studied as initial events (KE1), reactive oxygen species (ROS) and glutathione (GSH), Nrf2-Keap1 pathway (central detoxification pathway) and gene expression for KE2 as well as phenotypic modifications for KE3.We have shown that strong allergens are correlated with early production of FRO associated with GSH reduction. They also activate the Nrf2-Keap1 pathway and induce the expression of CD54 and CD86 cell surface markers as well as production of specific cytokines (IL-8, IL-18, etc.).To conclude, this work propose a new assay integrating all the biological measures as different KEs within the same cell.

Ce manuscrit présente l'intégration conjointe d'un banc de mesure de bruit de phase et de résonateurs BAW sur lesquels doit s'effectuer la mesure. Une tendance actuelle vise à intégrer à côté de systèmes plus ou moins complexes, des circuits permettant d'en faciliter les tests. L'intégration du banc de mesure de bruit de phase permet de nous affranchir des contraintes provenant de la mesure externe sous pointes et du coût élevé associé. L'intégration simultanée des circuits de tests avec les systèmes à mesurer, permet également d'exploiter pleinement les possibilités d'appariement de composants disponibles sur un même substrat. Ce type de mesure On-Chip simplifie considérablement la procédure de test, en minimisant l'utilisation de matériel de mesure externe encombrant et de coût élevé. Elle évite aussi les dispersions inhérentes à l'utilisation de composants discrets externes, offrant la possibilité de suivre facilement l'évolution des caractéristiques du système, soit dans le temps, soit après divers types de dégradations. Cette mesure intégrée conduit naturellement à la conception de circuits autotestables, et donc autoreconfigurables. Notre travail de thèse a consisté à définir l'architecture, ainsi que le dimensionnement des différents éléments du banc de mesure, en fonction de la précision de mesure souhaitée. Nous avons montrer qu'un système d'instrumentation performant peut s'intégrer dans une technologie SiGe standard.

A presença de antibióticos no ecossistema representa um sério risco à saúde humana e animal em virtude do desenvolvimento crescente de resistência bacteriana. Uma vez que a maioria dos antibióticos é persistente à degradação biológica, os processos oxidativos avançados são apontados como uma das tecnologias mais efetivas para decompor esses compostos em águas residuárias. A bacitracina zíncica (Bc-Zn) é um potente antibiótico constituído por uma mistura complexa de peptídeos não-biodegradáveis, conjugados ao zinco. Apesar de ser um antibiótico amplamente consumido na medicina humana e animal, é preocupante a escassez de estudos que investigam sua degradação e destino ambiental. O presente trabalho analisou a degradação da Bc-Zn através dos processos de fotólise direta e UV/H2O2 em diferentes condições de radiação UVC e concentração inicial de H2O2. Os parâmetros cinéticos rendimento quântico da fotólise, constantes cinéticas de pseudo-primeira ordem e constante cinéticas de segunda ordem foram satisfatoriamente estimados pela modelagem do sistema fotoquímico experimental. Os resultados revelaram que a fotólise direta permitiu degradar todos os congêneres da mistura de Bc-Zn nas maiores doses de radiação UVC empregadas. No entanto, não houve remoção de TOC após 120 minutos de irradiação. A adição de H2O2 acelerou substancialmente a fotodegradação do antibiótico, apresentando constantes cinéticas de pseudo-primeira ordem uma ordem de grandeza superiores às obtidas por fotólise direta. Além disso, remoção considerável de até 71% do TOC foi alcançada. A análise estatística demonstrou que a radiação UV foi um fator bem mais significativo para a fotodegradação da Bc-Zn em relação à concentração inicial de H2O2, sendo as melhores condições do processo alcançadas para a maior taxa específica de emissão de fótons (1,11×10-5 Einstein L-1 s-1). Ensaios biológicos com soluções tratadas por fotólise direta e UV/H2O2 indicaram remoção completa da atividade antimicrobiana residual, ainda que os produtos da fotodegradação tenham se mostrado não-biodegradáveis. Análises de toxicidade indicaram que o metal zinco presente no antibiótico é responsável pela a toxicidade no micro-organismo-teste Vibrio fischeri. Estudos adicionais devem ser realizados para identificar os sub-produtos formados, bem como para investigar a degradação da Bc-Zn em efluentes industriais reais. / The presence of antibiotics in ecosystems represents a serious risk to human and animal health, caused by the increase in bacterial resistance. Since most antibiotics resist to biological degradation, advanced oxidation processes are pointed out as the most effective technologies for degrading these compounds in wastewater. Zinc bacitracin (Bc-Zn) is a potent antibiotic with a complex mixture of non-biodegradable peptides conjugated to zinc. Despite being a widely used antibiotic in human and animal medicine, the scarcity of studies dealing with its degradation and environmental fate is a matter of concern. In this work, Bc-Zn degradation by direct photolysis and the UV/H2O2 process was investigated for different UVC radiation conditions and initial H2O2 concentrations. Kinetic parameters, namely the photolysis quantum yield, pseudo-first order kinetic constants and second-order kinetic constants, were satisfactorily estimated from experimental data by modeling the photochemical system. The results showed that all the congeners of the Bc-Zn mixture were photolyzed at the highest UVC doses applied, while no TOC removal was observed after 120 minutes of irradiation. The addition of H2O2 substantially accelerated Bc-Zn photodegradation, with pseudo-first order kinetic constants of one order of magnitude higher than those observed under direct photolysis. In addition, a remarkable removal of up to 71% of TOC was achieved. Statistical analyses showed that UV radiation had a much more important effect on Bc-Zn photodegradation in comparison with initial H2O2 concentration, with the best process conditions achieved for the highest specific photon emission rate (1.11×10-5 Einstein L-1 s-1). Biological assays carried out with the solutions treated by direct photolysis and UV/H2O2 revealed no residual antimicrobial activity, though photodegradation products remained non-biodegradable. In addition, toxicity analyses indicated that the zinc metal present in the antibiotic is responsible for the toxic effect on the test microorganism Vibrio fischeri. Finally, further studies should be performed to identify the by-products formed and to investigate Bc-Zn degradation in real industrial wastewater.

A presença de antibióticos no ecossistema representa um sério risco à saúde humana e animal em virtude do desenvolvimento crescente de resistência bacteriana. Uma vez que a maioria dos antibióticos é persistente à degradação biológica, os processos oxidativos avançados são apontados como uma das tecnologias mais efetivas para decompor esses compostos em águas residuárias. A bacitracina zíncica (Bc-Zn) é um potente antibiótico constituído por uma mistura complexa de peptídeos não-biodegradáveis, conjugados ao zinco. Apesar de ser um antibiótico amplamente consumido na medicina humana e animal, é preocupante a escassez de estudos que investigam sua degradação e destino ambiental. O presente trabalho analisou a degradação da Bc-Zn através dos processos de fotólise direta e UV/H2O2 em diferentes condições de radiação UVC e concentração inicial de H2O2. Os parâmetros cinéticos rendimento quântico da fotólise, constantes cinéticas de pseudo-primeira ordem e constante cinéticas de segunda ordem foram satisfatoriamente estimados pela modelagem do sistema fotoquímico experimental. Os resultados revelaram que a fotólise direta permitiu degradar todos os congêneres da mistura de Bc-Zn nas maiores doses de radiação UVC empregadas. No entanto, não houve remoção de TOC após 120 minutos de irradiação. A adição de H2O2 acelerou substancialmente a fotodegradação do antibiótico, apresentando constantes cinéticas de pseudo-primeira ordem uma ordem de grandeza superiores às obtidas por fotólise direta. Além disso, remoção considerável de até 71% do TOC foi alcançada. A análise estatística demonstrou que a radiação UV foi um fator bem mais significativo para a fotodegradação da Bc-Zn em relação à concentração inicial de H2O2, sendo as melhores condições do processo alcançadas para a maior taxa específica de emissão de fótons (1,11×10-5 Einstein L-1 s-1). Ensaios biológicos com soluções tratadas por fotólise direta e UV/H2O2 indicaram remoção completa da atividade antimicrobiana residual, ainda que os produtos da fotodegradação tenham se mostrado não-biodegradáveis. Análises de toxicidade indicaram que o metal zinco presente no antibiótico é responsável pela a toxicidade no micro-organismo-teste Vibrio fischeri. Estudos adicionais devem ser realizados para identificar os sub-produtos formados, bem como para investigar a degradação da Bc-Zn em efluentes industriais reais. / The presence of antibiotics in ecosystems represents a serious risk to human and animal health, caused by the increase in bacterial resistance. Since most antibiotics resist to biological degradation, advanced oxidation processes are pointed out as the most effective technologies for degrading these compounds in wastewater. Zinc bacitracin (Bc-Zn) is a potent antibiotic with a complex mixture of non-biodegradable peptides conjugated to zinc. Despite being a widely used antibiotic in human and animal medicine, the scarcity of studies dealing with its degradation and environmental fate is a matter of concern. In this work, Bc-Zn degradation by direct photolysis and the UV/H2O2 process was investigated for different UVC radiation conditions and initial H2O2 concentrations. Kinetic parameters, namely the photolysis quantum yield, pseudo-first order kinetic constants and second-order kinetic constants, were satisfactorily estimated from experimental data by modeling the photochemical system. The results showed that all the congeners of the Bc-Zn mixture were photolyzed at the highest UVC doses applied, while no TOC removal was observed after 120 minutes of irradiation. The addition of H2O2 substantially accelerated Bc-Zn photodegradation, with pseudo-first order kinetic constants of one order of magnitude higher than those observed under direct photolysis. In addition, a remarkable removal of up to 71% of TOC was achieved. Statistical analyses showed that UV radiation had a much more important effect on Bc-Zn photodegradation in comparison with initial H2O2 concentration, with the best process conditions achieved for the highest specific photon emission rate (1.11×10-5 Einstein L-1 s-1). Biological assays carried out with the solutions treated by direct photolysis and UV/H2O2 revealed no residual antimicrobial activity, though photodegradation products remained non-biodegradable. In addition, toxicity analyses indicated that the zinc metal present in the antibiotic is responsible for the toxic effect on the test microorganism Vibrio fischeri. Finally, further studies should be performed to identify the by-products formed and to investigate Bc-Zn degradation in real industrial wastewater.

O Paradigma Orientado a Objetos tem sido atualmente a abordagem dominante de desenvolvimento de software. Contudo, ela sofre da Tirania da Decomposição Dominante, pois não permite uma modularização adequada da implementação relativa a interesses estruturais. Como consequência, a implementação relativa a cada interesse estrutural fica espalhada pelos módulos do programa e entrelaçada com a implementação relativa a outros interesses estruturais. Outras abordagens de desenvolvimento de software, como o Desenvolvimento de Software Orientado a Aspectos com AspectJ e a Separação Multidimensional de Interesses em Hiperespaços com Hyper/J e CME, atingem sucesso moderado em oferecer mecanismos que permitem superar as deficiências do Paradigma Orientado a Objetos. No entanto, tais abordagens também possuem deficiências e omissões que devem ser reparadas para que elas possam se tornar utilizáveis em contextos típicos de desenvolvimento de software complexo. Este trabalho especifica uma nova abordagem, denominada Desenvolvimento de Software Orientado a Temas (DSOT), que tem como objetivo superar algumas deficiências das abordagens anteriores por meio de mecanismos que permitem a manipulação da implementação de cada interesse estrutural de forma separada e a manipulação da implementação de cada tipo de dado de forma separada. Além disso, DSOT possui operadores que são ortogonais, isto é, podem ser utilizados de forma combinada ou separada, para efetuar a composição de módulos do programa. Mostra-se o modelo conceitual do DSOT e descrevese um estudo de caso que consiste no desenvolvimento de um programa para demonstrar mais concretamente como o DSOT funciona na prática. Não se demonstra a superioridade do DSOT para o caso geral, mas os resultados alcançados evidenciam que o DSOT é uma abordagem promissora que merece ser investigada mais aprofundadamente em pesquisas futuras / The Object-Oriented Paradigm has currently been the dominant approach for developing software. However, it suffers from the Tyranny of the Dominant Decomposition, as it does not support a suitable modularization to the implementation relative to structural concerns. As a consequence, the implementation relative to each structural concern is scattered throughout the program modules and tangled with the implementation relative to other structural concerns. Some software development approaches, such as Aspect-Oriented Software Development with Aspect and Multidimensional Separation of Concerns in Hyperspaces with Hyper/J and CME, achieve moderate success in offering mechanisms that make it possible to overcome the deficiencies of the Object-Oriented Paradigm. However, such approaches also possess deficiencies and ommissions that must be corrected in order for them to get usable in typical complex software development contexts. This work specifies a new approach, named Theme- Oriented Software Development (TOSD), which aims at overcoming some deficiencies from previous approaches through mechanisms that support the handling of implementation for every structural concern separately and the handling of implementation for every data type separately. Moreover, TOSD contains operators which are orthogonal, that is, they can be used separately or as a combination, in order to perform composition of the program modules. We show the conceptual model of TOSD and describe a case study which consists in the development of a program to demonstrate more concretely how TOSD works in practice. We do not demonstrate the superiority of TOSD for the general case, but the results we have obtained suggest that TOSD is a promissing approach which deserves a deeper investigation in future research

O Paradigma Orientado a Objetos tem sido atualmente a abordagem dominante de desenvolvimento de software. Contudo, ela sofre da Tirania da Decomposição Dominante, pois não permite uma modularização adequada da implementação relativa a interesses estruturais. Como consequência, a implementação relativa a cada interesse estrutural fica espalhada pelos módulos do programa e entrelaçada com a implementação relativa a outros interesses estruturais. Outras abordagens de desenvolvimento de software, como o Desenvolvimento de Software Orientado a Aspectos com AspectJ e a Separação Multidimensional de Interesses em Hiperespaços com Hyper/J e CME, atingem sucesso moderado em oferecer mecanismos que permitem superar as deficiências do Paradigma Orientado a Objetos. No entanto, tais abordagens também possuem deficiências e omissões que devem ser reparadas para que elas possam se tornar utilizáveis em contextos típicos de desenvolvimento de software complexo. Este trabalho especifica uma nova abordagem, denominada Desenvolvimento de Software Orientado a Temas (DSOT), que tem como objetivo superar algumas deficiências das abordagens anteriores por meio de mecanismos que permitem a manipulação da implementação de cada interesse estrutural de forma separada e a manipulação da implementação de cada tipo de dado de forma separada. Além disso, DSOT possui operadores que são ortogonais, isto é, podem ser utilizados de forma combinada ou separada, para efetuar a composição de módulos do programa. Mostra-se o modelo conceitual do DSOT e descrevese um estudo de caso que consiste no desenvolvimento de um programa para demonstrar mais concretamente como o DSOT funciona na prática. Não se demonstra a superioridade do DSOT para o caso geral, mas os resultados alcançados evidenciam que o DSOT é uma abordagem promissora que merece ser investigada mais aprofundadamente em pesquisas futuras / The Object-Oriented Paradigm has currently been the dominant approach for developing software. However, it suffers from the Tyranny of the Dominant Decomposition, as it does not support a suitable modularization to the implementation relative to structural concerns. As a consequence, the implementation relative to each structural concern is scattered throughout the program modules and tangled with the implementation relative to other structural concerns. Some software development approaches, such as Aspect-Oriented Software Development with Aspect and Multidimensional Separation of Concerns in Hyperspaces with Hyper/J and CME, achieve moderate success in offering mechanisms that make it possible to overcome the deficiencies of the Object-Oriented Paradigm. However, such approaches also possess deficiencies and ommissions that must be corrected in order for them to get usable in typical complex software development contexts. This work specifies a new approach, named Theme- Oriented Software Development (TOSD), which aims at overcoming some deficiencies from previous approaches through mechanisms that support the handling of implementation for every structural concern separately and the handling of implementation for every data type separately. Moreover, TOSD contains operators which are orthogonal, that is, they can be used separately or as a combination, in order to perform composition of the program modules. We show the conceptual model of TOSD and describe a case study which consists in the development of a program to demonstrate more concretely how TOSD works in practice. We do not demonstrate the superiority of TOSD for the general case, but the results we have obtained suggest that TOSD is a promissing approach which deserves a deeper investigation in future research

Cette thèse s’inscrit dans le domaine de la modélisation des circuits analogiques et mixtes.Le travail part d’une problématique industrielle concernant les circuits électroniques utilisés dansles systèmes de forage pétrolier pour des besoins d’instrumentation et mesures. Ce travail de recherche concerne les circuits du front-end analogique que nous trouvons dans cette application industrielle. Nous examinons et nous essayons de trouver des modèles pour décrire l’effet des hautes températures sur les circuits électroniques dans un forage pétrolier. Ces circuits font partie des circuits industriels conventionnels. Ils ont généralement une température maximale de fonctionnement qui ne dépasse pas 125°C. Même si la température modifie le comportement de ces circuits, il existe des techniques d’adaptation qui permettent de compenser l’effet de la température sur ces circuits. Cependant, pour bien réussir la phase de la conception, il faut d’abord bien caractériser le comportement des différents circuits industriels utilisés en haute température. Il faut également trouver des modèles exacts qui décrivent le comportement de ces circuits en haute température. Or nous savons que la majorité des circuits industriels analogiques et mixtes sont décrits par des modèles de type SPICE. Par un choix de l’entreprise Schlumberger, notre partenaire industriel qui a financé ce travail, nous nous sommes intéressés dans notre étude à un composantspécifique présent dans la majorité des circuits analogiques et mixtes d’instrumentation :l’amplificateur opérationnel (l’AOP).Le travail commence par une étude des spécifications du circuit ainsi que le modèle SPICE.Une étude de la structure de ce modèle et sa simulation ont montré la non-précision du modèle audelàde 125°C. L’étude de validité du modèle a concerné le paramètre de la tension de décalage etle paramètre taux de rejection du mode commun. Nous avons interprété la différence des résultatsentre les mesures et la simulation de la tension de décalage. Nous avons constaté la limitation quereprésente l’approche structurelle par modélisation SPICE. Pour cette raison, nous avonssélectionné l’approche de modélisation comportementale pour les différents avantages qu’elleprésente. Ces avantages répondent à nos besoins et conviennent les mesures qui ont été effectuées.Nous avons sélectionné le langage VHDL-AMS et l’environnement Cadence ADVanceMS. Pourdéveloppé les modèles, nous avons alors énuméré les différents paramètres de performance d’unAOP. Nous avons validé la représentation de chaque paramètre par un circuit de test approprié.Dans un deuxième temps, nous avons approximé la variation de ces paramètres en température pardes équations polynomiales et exponentielles pour développer le modèle précis en HT. Le modèlea été validé par un circuit de test similaire au circuit expérimental. De bons résultats ont ététrouvés. L’erreur moyenne entre simulation VHDL-AMS et mesures n’a pas dépassé 3,11%. Dansle denier chapitre, nous avons simulé des circuits d’une chaine d’instrumentation. Nous avonssimulé l’effet de la température sur un capteur piézo-résistif (pont de Wheatstone). Trois architectures d’un amplificateur d’instrumentation ont été également modélisées e en se basant surle modèle VHD-AMS de l’AOP. / This work is dealing with the modelling of analogue and mixed signal circuits. Moreprecisely, we focus on modelling the circuits of an analogue front-end which is used in down-holedrilling industry for instrumentation and measurement purposes. This research had as a goal tomodel the temperature increasing effect in the behaviour of each circuit of the considered frontend.The studied circuits belong to the family of “conventional” circuits. Most of these circuitsoperate in a temperature which does not exceed 125°C. Even if the behaviour of the circuit changesdue to an increasing of the temperature, there are some well-know techniques that enable thecompensation of such effects. However, in order to obtain a precise simulation in the design phase,it is very important to have accurate models that describe the temperature increasing effect. Asmost of the commercial circuits models are written in SPICE, it is necessary first to review theaccuracy of SPICE models in high temperature (HT). This work focus on a specific circuit: theoperational amplifier (opamp). This device is present in many instrumentation circuits. Obtainingan accurate op-amp model in HT will help us develop accurate models of these circuits byconsidering their architectural description which is based on the opamp model.The work starts with the study of the structure of the SPICE model of the considered opamp.This study enables us to confirm the non-validity of the SPICE model in HT. The validity studyconsists in comparing the SPCE simulation results of two parameters (the voltage offset and thecommon mode rejection ratio) to measurement results. Moreover, we present an interpretation tothe difference that was observed in this comparison. After comparing different modellingapproaches, we select the behavioural modelling one. The VHDL-AMS was used to develop thenew precise opamp model in HT. The simulation is performance in Cadence/ADVanceMSenvironment. The representation of each opamp parameter is validated by a specific circuit. Thismodel is developed in two steps. In the first step, we develop an opamp model in which there is noconsideration of the temperature effect. In the second step, dependence of each parameter to thetemperature is described by a polynomial or exponential function. This function is the result of thefitting process of the measurement results. These equations are inserted in the VHDL-AMS model.All parameters are again validated in each temperature. The test-circuit is the same circuit used inthe experimental test of the opamp parameters. The average error between measurement andsimulation does not exceed 3.11%. In the last chapter, we simulate some circuits of the theanalogue front-end of an acquisition system. We simulate for example the effect of the temperatureeffect on the accuracy of a Wheatstone bridge. Three architecture of an instrumentation amplifierwere also modelled and simulated in different temperature of [20°C, 220°C] in the basis of thedeveloped opamp model.

Les attaques par canaux auxiliaires sont apparues dans la deuxième moitié des années 1990. Ces attaques exploitent différentes informations qu’il est possible de collecter lors de l’exécution d’un algorithme sur un appareil cryptographique. Il est ainsi possible, entre autres, de mesurer la consommation d’énergie d’un appareil cryptographique, ou encore d’observer le temps d’exécution d’un certain algorithme sur un appareil. C’est à ces deux sources d’in- formation que nous nous intéressons dans ce travail. Après une présentation des concepts utiles à la lecture du travail et de l’état de l’art des attaques et des contre-mesures du domaine, nous abordons les résultats de nos recherches effectuées lors de ce travail de thèse. Nous présentons d’abord nos contributions aux attaques par mesure de consommation d’énergie :(1) une approche com- binant apprentissage semi-supervisé et attaques par templates pour retrouver le poids de Hamming des différents bytes d’une clé de chiffrement et (2) une approche utilisant des techniques d’apprentissage automatique pour attaquer une implantation protégée d’AES. Ensuite, nous abordons les contre-mesures investiguées durant nos recherches qui se résument (1) en la possibilité de rendre l’ordre des instructions d’AES le plus aléatoire possible en jouant sur la relation de dépendance entre celles-ci ainsi qu’en (2) l’étude de l’application partielle (sur un sous-ensemble de données) de certaines contre-mesures, afin de protéger les données sensibles d’un algorithme. Enfin, nous terminons ce travail par l’emploi de la programmation orientée aspects comme manière d’implanter des contre-mesures pour les attaques temporelles (sur RSA) et pour les attaques par mesures de consommation d’énergie (sur AES). / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

Le secteur industriel du traitement de surface (TS), secteur d’excellence en Bourgogne Franche-Comté, est contraint de recourir à de nouvelles méthodes d’épuration des eaux du fait d’une règlementation européenne de plus en plus stricte. En effet, notamment dans le cadre de la Directive Cadre sur l’Eau, les industriels doivent sans cesse améliorer la qualité de leurs rejets et diminuer les impacts générés par ceux-ci. Ce travail de thèse s’inscrit dans cet objectif. Ainsi, trois grands types d’investigations ont été menés : le premier a permis de définir la composition qualitative et quantitative en substances dangereuses (SD) des rejets de TS et d’étudier leur variabilité temporelle ; le second a validé des modifications d’une station physico-chimique de traitement des eaux franc-comtoise pour diminuer le flux de SD ; le dernier a proposé des solutions innovantes par bioadsorption sur des matériaux de cyclodextrine et par procédé d’oxydation avancée (POA) soit par ozone/UV, soit par l’utilisation de catalyseurs Pd-Cu pour atteindre le même objectif.[...] / The industrial sector of surface treatment (ST), sector of excellence in Bourgogne Franche-Comté, is forced to resort to new methods for water treatment due to European regulations increasingly strict. Indeed, particularly in the context of the Water Framework Directive, industries must continually improve the quality of their discharge waters and reduce the impacts generated by them. This work is part of this. Thus, three types of investigations were conducted: the first has defined qualitative and quantitative composition of hazardous substances (HS) in ST discharge waters and study their temporal variability; the second has approved changes in a physicochemical wastewater decontamination station to decrease HS water flow; the last proposed innovative solutions by biosorption on cyclodextrin materials and advanced oxidation process (AOP) by ozone / UV, or by the use of Pd-Cu catalysts to achieve the same objective.[...]