Made available in DSpace on 2014-12-17T14:07:09Z (GMT). No. of bitstreams: 1 HaroldoRAM_TESE.pdf: 4102900 bytes, checksum: e74f8a9122bb5cd84cbeadae3a476ce1 (MD5) Previous issue date: 2012-02-03 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The titanium and titanium alloys are widely used as biomaterial in biomedical device and so research have been developed aiming to improve and/or better to understand interaction biomaterial/biological environment. The process for manufacturing of this titanium implants usually involves a series of thermal and mechanical processes which have consequence on the final product. The heat treatments are usually used to obtain different properties for each application. In order to understand the influence of these treatments on the biological response of the surface, it was done, in this work, different heat treatments in titanium and analyzed their influence on the morphology, adhesion and proliferation of the pre-osteoblastic cells (MC3T3-E1). For such heat-treated titanium disks were characterized by optical microscopy, contact angle, surface energy, roughness, microhardness, X-ray diffraction and scanning through the techniques (BSE, EDS and EBSD). For the analysis of biological response were tested by MTT proliferation, adhesion by crystal violet and β1 integrin expression by flow cytometry. It was found that the presence of a microstructure very orderly, defined by a chemical attack, cells tend to stretch in the same direction of orientation of the material microstructure. When this order does not happen, the most important factor influencing cell proliferation is the residual stress, indicated by the hardness of the material. This way the disks with the highest level state of residual stress also showed increased cell proliferation / O tit?nio e suas ligas s?o amplamente utilizados como biomaterial em dispositivos biom?dicos e devido a isso pesquisas t?m sido desenvolvidas visando aperfei?oar e/ou compreender melhor a intera??o biomaterial/meio biol?gico. O processo de fabrica??o desses dispositivos de tit?nio geralmente envolve uma s?rie de processos t?rmicos e mec?nicos e que t?m consequ?ncias no produto final. Os tratamentos t?rmicos s?o usualmente utilizados para obten??o de propriedades diferenciadas para cada aplica??o. Com o intuito de entender a influ?ncia desses tratamentos sobre a resposta biol?gica da superf?cie, foram realizados, no presente trabalho, diferentes tratamentos t?rmicos em tit?nio e analisadas suas influ?ncias na morfologia, ades?o e prolifera??o de c?lulas pr?-osteobl?stica (MC3T3-E1). Para tanto os discos de tit?nio tratados termicamente foram caracterizados por microscopia ?tica, ?ngulo de contato, energia de superf?cie, rugosidade, microdureza Vickers, difra??o de raios-X e microscopia eletr?nica de varredura atrav?s das t?cnicas de EBS, EDS e EBSD. Para an?lise da resposta biol?gica foram realizados teste de prolifera??o por MTT, ades?o por cristal violeta e express?o da integrina β1 por citometria de fluxo. Foi verificado que na presen?a de uma microestrutura muito ordenada, definida atrav?s de um ataque qu?mico, as c?lulas tendem a se alongar no mesmo sentido da orienta??o microestrutural do material. Quando essa ordem n?o acontece, o fator mais importante a influenciar na prolifera??o celular ? a tens?o residual, indicada pela dureza do material. Deste modo os discos que apresentaram maior estado de tens?o residual apresentaram tamb?m maior prolifera??o celular

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 lopes.pdf: 4584389 bytes, checksum: 490508b2570ae9d7329524ffc42068ef (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:36:20Z (GMT) No. of bitstreams: 5 lopes.pdf: 4584389 bytes, checksum: 490508b2570ae9d7329524ffc42068ef (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:36:20Z (GMT). No. of bitstreams: 5 lopes.pdf: 4584389 bytes, checksum: 490508b2570ae9d7329524ffc42068ef (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / A presente disserta??o consiste em dois artigos cient?ficos, sendo um artigo original e outro de revis?o. Em um dos artigos, os autores fizeram uma revis?o da literatura sobre c?ncer e pr?polis, abordando a carcinog?nese experimental induzida quimicamente pelo 9,10-dimetil-1,2-benzantraceno (DMBA), a composi??o qu?mica da pr?polis e seus efeitos quimiopreventivos. O objetivo do artigo de pesquisa foi avaliar a rea??o tecidual da mucosa lingual de hamsters, submetida ? aplica??o di?ria e alternada de DMBA e extrato etan?lico de pr?polis (EEP) de uma apresenta??o comercial. Materiais e m?todos: 60 hamsters foram divididos em tr?s grupos, com dois per?odos experimentais, 13 e 20 semanas. A borda lateral da l?ngua foi submetida ? aplica??o t?pica, di?ria e alternada de DMBA 0,5 % e EEP 30 % (grupo EEP, n = 20), DMBA 0,5 % e extrato aquoso de pr?polis (EAP) (grupo EAP, n = 20), DMBA 0,5 % e soro fisiol?gico (grupo DMBA, n = 20). Realizou-se an?lise da ocorr?ncia dos tipos de altera??es cl?nicas e histol?gicas. Mensurou-se a ?rea e volume das altera??es cl?nicas, a ocorr?ncia das altera??es estruturais (AE) e citol?gicas (AC) do tecido epitelial escamoso com atipias e a ?rea histol?gica dos carcinomas de c?lulas escamosas (CCE). Para a an?lise estat?stica, aplicaram-se os testes ANOVA, qui-quadrado, Kruskal-Wallis e Mann-Whitney. Resultados: Para todas as vari?veis analisadas, n?o houve diferen?as significativas na compara??o entre os grupos nos dois tempos experimentais. Em 13 semanas ocorreu uma ?nica les?o de CCE no grupo EEP. Em 20 semanas, a maior ocorr?ncia de CCE tamb?m foi no grupo EEP. Conclus?o: o EEP, com teor alco?lico de 30 %, favoreceu a rea??o tecidual da inicia??o e da promo??o da carcinog?nese, por mecanismos ainda n?o elucidados. / ABSTRACT This dissertation consists of two papers, the first one is a review and the second is an original paper. In the first article, the authors reviewed the literature on cancer and propolis, addressing experimental carcinogenesis, chemically induced by 9,10-dimethyl-1,2-benzanthracene (DMBA), the chemical composition of propolis and its chemopreventive effects. The aim of the research paper was to evaluate the?tissue response?of tongue mucosa of hamsters, subjected to daily and alternating application of ethanolic extract of propolis (EEP) of a commercial presentation. Materials and methods: 60 hamsters were divided into three groups, and two experimental periods, 13 and 20 weeks. The lateral edge of the tongue underwent daily and alternate topical application of DMBA 0.5% and 30% EEP (EEP group, n = 20), DMBA and 0.5% aqueous extract of propolis (EAP) (EAP group; n = 20), and DMBA 0.5%?saline (DMBA?group, n =?20). The occurrence of types of clinical and histological changes was analysed. The area and volume of clinical changes, the occurrence of structural (AE) and cytological (CA) changes of squamous epithelial tissue with atypia and the histological area of squamous cell carcinoma (SCC) were measured. For statistical analysis, ANOVA, chi-square and Kruskal-Wallis and Mann-Whitney test were applied. Results: For all variables, there were no significant differences when comparing the groups in the two experimental periods. In 13 weeks, a single lesion of SCC in EEP group was observed. At 20 weeks, the highest occurrence of SCC was also in the group EEP. Conclusion: the EEP, with an alcohol content of 30% favored tissue reaction of initiation and promotion of carcinogenesis by mechanisms not yet elucidated.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 paulo.pdf: 587935 bytes, checksum: 87aa85ba53d81494c9eabf55750f07b7 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:41:39Z (GMT) No. of bitstreams: 5 paulo.pdf: 587935 bytes, checksum: 87aa85ba53d81494c9eabf55750f07b7 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:41:39Z (GMT). No. of bitstreams: 5 paulo.pdf: 587935 bytes, checksum: 87aa85ba53d81494c9eabf55750f07b7 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Uma sa?de bucal prec?ria pode afetar aspectos da vida di?ria das crian?as, como a capacidade mastigat?ria, o sono, o desempenho escolar, a intera??o social, a autoestima, bem como o peso e o crescimento. No entanto, h? poucas evid?ncias sobre a influ?ncia de problemas bucais no bem-estar biopsicossocial de pr?-escolares e suas fam?lias, sobretudo comparando aqueles que vivem em zonas urbanas e rurais. O objetivo deste estudo foi avaliar os fatores determinantes da qualidade de vida relacionada ? sa?de bucal (OHRQoL) de crian?as pr?-escolares e suas fam?lias residentes em zona urbana e em zonas rurais de extrema pobreza. Foi realizado um estudo transversal com 391 crian?as, entre dois e cinco anos de idade, das zonas urbana e rural de Diamantina/MG, selecionadas aleatoriamente durante a Campanha Nacional de Vacina??o Infantil. Os pais das crian?as responderam ? vers?o brasileira do Early Childhood Oral Health Impact Scale (B-ECOHIS) e forneceram dados socioecon?micos. Exames cl?nicos das crian?as foram realizados para avaliar c?rie dent?ria, defeitos de desenvolvimento de esmalte, fluorose dent?ria, les?es ou varia??es de normalidade da mucosa bucal e m? oclus?o. An?lise estat?stica envolveu an?lise descritiva, teste de Mann-Whitney, teste do qui-quadrado e regress?o linear m?ltipla. O n?vel de signific?ncia estabelecido foi de 5%. Os valores m?dios do ECOHIS para cada condi??o bucal avaliada foram: c?rie dent?ria 4.52 (DP=6.84), defeitos de desenvolvimento de esmalte 3,82 (DP=6,86), fluorose dent?ria 4,56 (DP=7,53), les?es ou varia??es da normalidade da mucosa bucal 2,84 (DP=5,46) e m? oclus?o 2,16 (DP=4,56). Diferen?as significativas foram encontradas entre o local de resid?ncia e: g?nero (P = 0,008), n?mero de filhos (P = 0,001) e defeitos de desenvolvimento de esmalte (P = 0,035). Fam?lias da zona rural apresentaram valores significativamente inferiores nos aspectos socioecon?micos (P < 0,005), quando comparadas ? fam?lias da zona urbana. Na zona urbana, foram encontradas associa??es significativas entre impacto na OHRQoL e: idade da crian?a em meses (P = 0,004), g?nero (feminino) (P = 0,034), n?mero de dentes cavitados (P < 0,001) e presen?a de defeitos de desenvolvimento de esmalte (P = 0,004). Na zona rural foi encontrada associa??o significativa somente entre impacto na OHRQoL e n?mero de dentes cavitados (P <0,001). Problemas bucais como c?rie dent?ria e defeitos de desenvolvimento de esmalte afetaram negativamente a qualidade de vida de crian?as pr?-escolares e suas fam?lias. Na zona urbana, o n?mero de dentes cavitados, defeitos de desenvolvimento de esmalte, al?m da idade e g?nero (feminino) contribu?ram para diminui??o da qualidade de vida das crian?as pr?-escolares e suas fam?lias. Na zona rural, somente o n?mero de dentes cavitados comprometeu significativamente a vida di?ria dos pr?-escolares e suas fam?lias. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT Poor oral health can affect aspects of children's daily lives, such as the ability to chew, sleep, school performance, social interaction, self-esteem as well as weight and growth. However, there is little evidence about the influence of oral health problems on biopsychosocial well-being of preschool children and their families, especially comparing those living in urban and rural areas. The aim of this study was to assess the determinants of oral health-related quality of life (OHRQoL) of preschool children and their families living in urban and rural areas of extreme poverty. A cross-sectional study was conducted with 391 children between two and five years of age, from urban and rural areas in Diamantina/MG randomly selected during the National Immunization Campaign. The children's parents answered the Brazilian version of the Early Childhood Oral Health Impact Scale (B-ECOHIS) and provided socioeconomic data. Clinical examination of the children was conducted to assess dental caries, developmental defects of enamel, dental fluorosis, injury or damage to the oral mucosa and malocclusion. Statistical analysis involved descriptive analysis, Mann-Whitney test, chi-square test and multiple linear regression. Significance level was set at 5%. The mean ECOHIS total scores for each oral condition were: dental caries 4.52 (SD = 6.84), developmental defects of enamel, 3.82 (SD = 6.86), dental fluorosis 4:56 (SD = 7.53), injury or oral mucosa lesions 2.84 (SD = 5.46) and malocclusion 2.16 (SD = 4.56). Significant differences were found between place of residence and: gender (P = 0.008), number of children (P = 0.001) and developmental defects of enamel (P = 0.035). Families from rural area had significantly lower socioeconomic aspects (P <0.005), when compared to families from urban area. In urban areas, significant associations were found between the impact on OHRQoL and children's age in months (P = 0.004), gender (female) (P = 0.034), number of cavitated teeth (P <0.001) and presence of developmental defects of enamel (P = 0.004). In rural areas significant association was found only between the impact on OHRQoL and the number of cavitated teeth (P <0.001). Oral health problems such as dental caries and developmental defects of enamel negatively affected the quality of life of preschool children and their families. In the urban area, number of cavitated teeth, developmental defects of enamel, in addition to age and gender contributed to decreased quality of life of preschool children and their families. In rural areas, only the number of cavitated teeth impaired significantly the daily lives of preschool children and their families.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 george.pdf: 1321962 bytes, checksum: 4431a511a9e919efedb58883c89d3635 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:47:26Z (GMT) No. of bitstreams: 5 george.pdf: 1321962 bytes, checksum: 4431a511a9e919efedb58883c89d3635 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:47:26Z (GMT). No. of bitstreams: 5 george.pdf: 1321962 bytes, checksum: 4431a511a9e919efedb58883c89d3635 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / O objetivo deste estudo foi avaliar a influ?ncia da qualidade do tratamento de canal radicular (TCR), de fatores relacionados ? restaura??o coron?ria (RC), les?es por c?rie e par?metros cl?nicos periodontais no estado periapical de dentes tratados endodonticamente. Os pacientes foram tratados por estudantes de gradua??o na Cl?nica de Endodontia da Universidade Federal dos Vales do Jequitinhonha e Mucuri, entre o per?odo de 2004 a 2009. A reavalia??o cl?nica e radiogr?fica foi realizada entre Setembro de 2010 e Fevereiro de 2011. Foram coletados dados contidos nos prontu?rios odontol?gicos (idade, g?nero, dente tratado, estado periapical pr?-operat?rio, tipo de tratamento, t?cnicas de instrumenta??o e obtura??o empregadas, radiografia final da obtura??o e tipo de restaura??o coron?ria). Clinicamente, foram considerados fatores relacionados a sinais e sintomas endod?nticos, les?es por c?rie, RC e par?metros periodontais. Radiograficamente avaliou-se o estado periapical, les?es por c?rie, RC e pino intrarradicular. Os resultados foram analisados estatisticamente usando os testes qui-quadrado de Pearson, exato de Fisher, McNemar e regress?o log?stica bivariada e multivariada (p<0,05). Foram reavaliados 122 pacientes ap?s per?odo de acompanhamento de 12 a 66 (37,7?16,4) meses, e estes apresentaram 154 dentes tratados endodonticamente. Os dados foram organizados e analisados conforme o desenho de estudo e vari?veis estudadas, sendo apresentados em dois artigos cient?ficos. No geral a taxa de sucesso cl?nico-radiogr?fico do TCR foi de 73,4%. No estudo longitudinal observou-se frequ?ncias elevadas de obtura??es perfeitas (41,6%) e satisfat?rias (46,1%) e frequ?ncia reduzida de obtura??es deficientes (12,3%). A qualidade das obtura??es e das restaura??es coron?rias, analisadas isoladamente, influenciou no sucesso cl?nico-radiogr?fico (p<0,05); a presen?a de pr?via les?o periapical (OR=10,24; IC=3,15-33,22) foi o fator decisivo para o insucesso do tratamento. No estudo transversal observou-se que a presen?a de les?es por c?rie (p=0,017), sangramento gengival ? sondagem (p=0,043) e mobilidade dent?ria (p=0,022) foram os fatores associados ao insucesso do TCR. As outras vari?veis relacionadas ?s les?es por c?rie, RC, qualidade da obtura??o e par?metros cl?nicos periodontais, n?o influenciaram no insucesso do tratamento (p>0,05). Concluiu-se que, no estudo longitudinal, o pr?vio estado periapical foi o principal fator que influenciou o progn?stico do TCR e, no estudo transversal, a presen?a de les?es por c?rie e aspectos periodontais influenciaram negativamente no estado periapical dos dentes tratados endodonticamente. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The aim of this study was to evaluate the influence of root canal treatment (RCT) quality, factors related to the coronal restoration (CR), carious lesions and periodontal clinical parameters in the periapical status of endodontically treated teeth. The patients were treated by undergraduate students at the Clinic of Endodontics, Federal University of Jequitinhonha and Mucuri Valleys (UFVJM) in the period between 2004-2009. The clinical and radiographic recall was performed between September 2010 and February 2011. Data were collected in records dentistry (age, gender, treated tooth, periapical status preoperative, type of treatment, instrumentation and obturation techniques, final radiography of the filling and the type of coronal restoration). Clinically, factors related to carious lesions, CR and periodontal parameters were considered. Radiographic parameters were evaluated the periapical status, carious lesions, CR and cast post. The results were statistically analyzed using Pearson chi-square, Fisher?s exact and McNemar tests, bivariate and multivariate logistic regression (p<0.05). A total of 122 patients were reassessed after a follow-up of 12-66 (37.7?16.4) months which had 154 endodontically treated teeth. Data were organized and analyzed according to the study design and variables, and are presented in two papers. Overall, the success rate of the RCT was 73.4%. In the longitudinal study it was observed a high frequency of perfect (41.6%) and satisfactory (46.1%) fillings and a reduced frequency of deficient (12.3%) fillings. The quality of fillings and coronal restorations, when analyzed individually, influenced the clinical and radiographic success (p<0,05); preoperative periapical lesion was the main factor for the treatment insuccess (OR=10.24, CI=3.15-33.22). In cross-sectional study it was observed that the presence of carious lesions (p=0.017), gingival bleeding (p=0,043) and tooth mobility (p=0.022) were the factors associated with insuccess of RCT. The other variables related to carious lesions, CR, quality of fillings and periodontal clinical parameters, did not influence the insuccess treatment (p>0.05). In conclusion, the prior periapical status was the main factor affecting the prognosis of the RCT in the longitudinal study and, in the cross-sectional study the presence of carious lesions and periodontal aspects negatively influenced the periapical status of endodontically treated teeth.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:03:29Z No. of bitstreams: 5 lindomar.pdf: 4197857 bytes, checksum: 529e21e13238b7b71e5ddffb7f4e7b91 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2111 bytes, checksum: f5c843397da71f5d32b775fd8c4cbf56 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T09:29:04Z (GMT) No. of bitstreams: 5 lindomar.pdf: 4197857 bytes, checksum: 529e21e13238b7b71e5ddffb7f4e7b91 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2111 bytes, checksum: f5c843397da71f5d32b775fd8c4cbf56 (MD5) / Made available in DSpace on 2015-02-20T09:29:04Z (GMT). No. of bitstreams: 5 lindomar.pdf: 4197857 bytes, checksum: 529e21e13238b7b71e5ddffb7f4e7b91 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2111 bytes, checksum: f5c843397da71f5d32b775fd8c4cbf56 (MD5) Previous issue date: 2013-12-17 / Eletrodos constitu?dos de ?-PbO2 foram preparados via eletrodeposi??o, mediante inclus?o dos ?ons Fe3+ e F- na mistura precursora, usando como substratos tela de a?o (ASTM 316) e tecido de carbono, visando a produ??o eletroqu?mica de oz?nio (PEO) em um reator filtro-prensa conduzida na aus?ncia de eletr?lito l?quido. A an?lise das micrografias de MEV para ambos substratos revelaram que em baixas concentra??es dos dopantes houve a forma??o de gr?os bem definidos com uma ?rea superficial uniforme. A an?lise de Raios-X para ambos substratos revelou o desfavorecimento da forma??o da fase ?-PbO2 para a maioria da concentra??o dos dopantes. Estudos cronopotenciom?tricos evidenciaram a estabilidade dos anodos quando sujeitados a intensa evolu??o de gases. Para o eletrodo dopado com 1 mM do ?on Fe3+, a efici?ncia de corrente da PEO foram de 10% e 9,5% com uma velocidade de gera??o de oz?nio de 1,35 g h-1 e 1,40 g h-1 para os substratos de tela de a?o e de tecido de carbono, respectivamente. Os resultados encontrados s?o promissores indicando uma viabilidade do uso desses eletrodos para aplica??o no tratamento de ?gua, por isso, empregou-se o oz?nio produzido eletroquimicamente para tratar ?gua residu?ria proveniente da produ??o de biodiesel na planta piloto da UFVJM. O tratamento oxidativo da ?gua residu?ria foi conduzido num reator em coluna de bolhas via rea??o direta (oz?nio molecular), indireta (radical hidroxila) e mista (O3/HO?) manipulando-se o pH do meio reacional.A caracteriza??o do efluente ozonizado foi efetuada monitorando-se o processo de degrada??o da mat?ria org?nica dissolvida via espectrofotometria. A cin?tica do processo de degrada??o seguiu o modelo de pseudo-primeira ordem. Foi verificado que a constante cin?tica sofre varia??o com o tempo de ozoniza??o revelando, portanto, uma varia??o da recalcitr?ncia com o tempo de rea??o. A demanda de energia requerida no processo de ozoniza??o foi caracterizada por uma energia el?trica por ordem (EEO) de 139 kW h m-3 ordem-1. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Electrodes comprised of ?-PbO2 were prepared by electrodeposition, by moans of the inclusion of Fe3+ and F- ions in the precursor mixture, using as substrates steel screen (ASTM 316) and carbon cloth in order to produce ozone in an filter-press electrochemical reactor conducted in the absence of liquid electrolyte. The analysis of SEM micrographs revealed that for both substrates at low dopants concentrations resulted in the formation of well-defined grains with a uniform surface area. X-rays analysis for both substrates showed the inhibition of formation of the ?-PbO2 phase for most of the dopant concentration. Cronopotenciometric studies revealed a good stability of the anodes when they were subjected to the intense gas evolution. For the electrode doped with 1 mM Fe3+, the current efficiency of EOP were 10% and 9.5% for an ozone generation rate of 1.35 g h-1 and 1.40 g h -1 using the steel screen and carbon cloth substrates, respectively. These results are promising, indicating a feasibility of using these electrodes for application in water purification, so the electrochemical ozone produced was applied to treat residuary water obtained from the biodiesel produced in the pilot plant of UFVJM. The oxidative chemical treatment of wastewater was conducted in a bubble column reactor via direct (molecular ozone), indirect (hydroxyl radical) and mixed (O3/HO?) oxidation reaction thrush manipulating the pH of the reaction medium. The characterization of ozonated effluent was assessed by means of monitoring the degradation of the dissolved organic matter using spectrophotometry. The kinetics of the degradation process followed a pseudo-first order model. It was found that the rate constant changes on the ozonation time thus indicating a recalcitrance variation with the reaction time. The energy demand required in the ozonation process was described by an electric energy per order (EEO) of 139 kW h m-3 order-1.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-12-19T15:57:03Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) vitor_antunes_martins_costa.pdf: 1528193 bytes, checksum: f3bfb92e98d475f9f7d9906fb470b3cd (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-01-03T17:19:33Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) vitor_antunes_martins_costa.pdf: 1528193 bytes, checksum: f3bfb92e98d475f9f7d9906fb470b3cd (MD5) / Made available in DSpace on 2018-01-03T17:19:33Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) vitor_antunes_martins_costa.pdf: 1528193 bytes, checksum: f3bfb92e98d475f9f7d9906fb470b3cd (MD5) Previous issue date: 2017 / O estabelecimento de esp?cies ex?ticas em ?reas degradados p?s fogo ? recorrente na Mata Atl?ntica. Estas esp?cies modificam a estrutura e fun??o do ecossistema resultando em significativa altera??o no fornecimento dos servi?os ecossist?micos. Assim, formas de recupera??o das condi??es nativas para desenvolver o ecossistema que existia anteriormente s?o aplicadas. Desta forma, em uma ?rea de Mata Atl?ntica com hist?rico de dist?rbio provocado por inc?ndio seguido de elevada infesta??o de plantas ex?ticas avaliou-se a sobreviv?ncia e desenvolvimentos de esp?cies nativas transplantadas na ?rea e o comportamento das plantas ex?ticas ap?s a aplica??o dos tratamentos, em dois experimentos. Utilizou-se as seguintes esp?cies: Tapirira guianenses Aubl, Platycyamus regnelli Benth, Inga sessilis (Vell.) Mart., Enterolobium contortisiliquum (Vell.) Morong., Anadenanthera colubrina (Vell.) Brenan, Copaifera langsdorffii Desf, Dalbergia nigra (Vell.) Fr. All. Ex Benth, , Hymenaea courbaril L., Melanoxylon brauna Schott VU, Joanesia princeps Vell., e Eugenia uniflora L. Avaliou-se sobreviv?ncia, altura, di?metro ao n?vel do solo, ?rea de copa das mudas transplantadas e cobertura das esp?cies ex?ticas (Samambaia e Capim meloso). O experimento 1, que consistia de esp?cies Pioneiras, foi plantado nas densidades de 2000, 2500 e 4000 plantas ha-1. O experimento 2, que consistia de esp?cies de grupos distintos, foi implantado nas densidades de 2000, 3000 e 4000 plantas ha-1. Para os tratamentos, combinou-se formas de remo??o das esp?cies ex?ticas (gradagem ou ro?ada) nas diferentes densidades de plantio. Entre as duas avali??es realizadas, a maior sobreviv?ncia das esp?cies nativas foi registrada na primeira avalia??o. No entanto, para altura, di?metro ao n?vel do solo e ?rea de copa as maiores m?dias foram registradas na segunda avalia??o. Em rela??o ?s formas de remo??o, a utiliza??o da gradagem tem promovido maior sobreviv?ncia das mudas. As esp?cies ex?ticas est?o em alta densidade na ?rea. Em algumas parcelas, estas ocupam quase 100% do solo. O plantio sofreu aumento de mortalidade decorrente do per?odo de estiagem entre as avalia??es. A samambaia, adaptada a condi??es de elevado pH e baixa qualidade nutricional, pode ter alterado o ambiente e proporcionado o desenvolvimento de capim meloso. Esp?cies pioneiras, como Anadenanthera colubrina, pode ser uma competidora equivalente das esp?cies ex?ticas, visto seu r?pido crescimento e boa adapta??o ?s condi??es locais impostas. Verificou-se necessidade de controle das plantas daninhas, mesmo em parcelas onde as esp?cies arb?reas est?o se desenvolvendo bem, em fun??o do aumento de densidade das infestantes. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The establishment of exotic species in degraded areas after fire is recurrent in the Atlantic Forest. These species modify the structure and function of the ecosystem resulting in a significant change in the provision of ecosystem services. Thus, ways of recovering the native conditions to develop the ecosystem that existed previously are applied. Thus, in an area of Atlantic Forest with a history of fire-induced disturbance followed by high infestation of exotic plants the survival and development of native species transplanted in the area and the behavior of exotic plants after the application of the treatments were evaluated in two experiments. The following species were used: Tapirira guianenses Aubl, Platycyamus regnelli Benth, Inga sessilis (Vell.) Mart., Enterolobium contortisiliquum (Vell.) Morong., Anadenanthera colubrina (Vell.) Brenan, Copaifera langsdorffii Desf, Dalbergia nigra (Vell.) Fr. All. Ex Benth., Hymenaea courbaril L., Melanoxylon brauna Schott VU, Joanesia princeps Vell., and Eugenia uniflora L. survival, height, diameter at ground level, crown area of transplanted seedlings and cover of exotic species (Fern and Mellow Grass). Experiment 1, which consisted of Pioneer species, was planted at densities of 2000, 2500 and 4000 plants ha-1. Experiment 2, which consisted of species of distinct groups, was implanted at densities of 2000, 3000 and 4000 plants ha-1. For the treatments, forms of removal of the exotic species (harrowing or mowing) at the different planting densities were combined. Among the two evaluations, the highest survival of native species was recorded in the first evaluation. However, for height, diameter at ground level and crown area the highest averages were recorded in the second evaluation. Regarding the forms of removal, the use of harrowing has promoted greater survival of the seedlings. Exotic species are in high density in the area. In some plots, these occupy almost 100% of the soil. Planting increased mortality due to the dry season between evaluations. The fern, adapted to conditions of high pH and low nutritional quality, may have altered the environment and provided the development of mellow grass. Pioneering species, such as Anadenanthera colubrina, may be an equivalent competitor of exotic species because of their rapid growth and good adaptation to local conditions imposed. There was a need for weed control, even in plots where tree species are developing well, due to the increase in weeds density.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-30T15:49:24Z No. of bitstreams: 1 2010 - Juliana M?ller Freire.pdf: 12006121 bytes, checksum: 145a6bd3be51d71faac2dfdc0a20e5c4 (MD5) / Made available in DSpace on 2016-08-30T15:49:24Z (GMT). No. of bitstreams: 1 2010 - Juliana M?ller Freire.pdf: 12006121 bytes, checksum: 145a6bd3be51d71faac2dfdc0a20e5c4 (MD5) Previous issue date: 2010-07-01 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / This study aimed to evaluate the structure and floristics of shrubs and trees in areas of northern and southern slopes in the southern portion of the Maci?o da Pedra Branca, Rio de Janeiro, RJ. We tested the hypothesis that different component orientations may influence the structure and floristic changes. In a sample area of 9.700 m2, divided into ten areas, were surveyed 1508 individuals, distributed in 324 species, 154 genera and 52 families. The families richest in species were: Myrtaceae, Lauraceae, Fabaceae, Rubiaceae and Euphorbiaceae. The Diversity Index Shannon-Wiener (H ') was 5.093 nats / indiv., And the evenness (J) was 0.881. Horizontal structure in the species Joannesia princeps, Piptadenia gonoacantha e Pseudopiptadenia contorta, Meternichia princeps, Apuleia leiocarpa, Astrocaryum aculeatissimum, Chrysophyllum flexuosum reached the highest value of phytosociological importance. A phytosociological analysis of each individual area indicated significant floristic and structural variations, with a trend toward greater diversity is found in areas of higher altitude and farther from the urban perimeter. Of the 324 species found in the study, 124 were found exclusively on the north side, 100 on the southern slope and 100 occurred in both strands. The exclusive species and more frequent on the north side were Brosimum guianense e Annona cacans, Zollernia ilicifolia, Couratari pyramidata, Lecythis pisonis e Gallesia integrifolia. On the southern slope, the exclusive species that stood out were Cariniana estrellensis, Cabralea canjerana, Eugenia microcarpa. Cluster analysis among the ten areas indicated, in general, greater similarity due to the proximity between areas than for the orientation of the slope, however comparing the north and south closer together, it is observed that the highest diversity indices, most proportion of rare species and unique and the most advanced stages of succession were found on the southern slope. The cases where this did not happen were observed in areas closer to the urban boundaries that regardless of the slope were less floristic diversity. The diameter distribution model presented inverted J, with a diameter range quite variable between areas. No pattern was found between the diameter distribution and orientation of the slope. Comparing the flora of Pedra Branca with other forest areas in Rio de Janeiro, there was generally a low floristic similarity. The major floristic identity occurred with the work in-house Pedra Branca, Serra da Tiririca, and Tijuca Forest. Works out of the city showed floristic similarity below 25%. The high diversity of the studied area is a reflection of its high environmental heterogeneity, related to differences in altitude, slope orientation, successional stage and history of use, which puts the Pedra Branca as a strategic area for species conservation in Rio de Janeiro. / Este trabalho teve como objetivo avaliar a estrutura e a flor?stica do estrato arbustivo e arb?reo em ?reas de encostas norte e sul, na por??o meridional do maci?o da Pedra Branca, Rio de Janeiro, RJ. Foi testada a hip?tese que diferentes orienta??es de vertente podem influenciar a estrutura e a flor?stica da vegeta??o. Em uma ?rea amostral de 9.700 m2, distribu?das em dez ?reas, foram inventariados 1.508 indiv?duos, distribu?dos em 324 esp?cies, 154 g?neros e 52 fam?lias. As fam?lias mais ricas em esp?cies na foram: Myrtaceae, Lauraceae, Fabaceae, Rubiaceae e Euphorbiaceae. O ?ndice de Diversidade de Shannon- Wiener (H?) foi de 5,093 nats/indiv., e a equabilidade (J) foi de 0,881. Na estrutura horizontal as esp?cies Joannesia princeps, Piptadenia gonoacantha e Pseudopiptadenia contorta, Meternichia princeps, Apuleia leiocarpa, Astrocaryum aculeatissimum, Chrysophyllum flexuosum alcan?aram o maior valor de import?ncia fitossociol?gico. A an?lise fitossociol?gica de cada ?rea indicou importantes varia??es estruturais e flor?sticas, com tend?ncia ? maior diversidade ser encontrada nas ?reas de maior altitude e mais distantes do per?metro urbano. Das 324 esp?cies encontradas no trabalho, 124 ocorreram exclusivamente na vertente norte, 100 na vertente sul e 100 ocorreram em ambas as vertentes. As esp?cies exclusivas e mais freq?ente na vertente norte foram o Brosimum guianense e Annona cacans, Zollernia ilicifolia, Couratari pyramidata, Lecythis pisonis e Gallesia integrifolia. Na vertente sul, as esp?cies exclusivas que mais se destacaram foram Cariniana estrellensis, Cabralea canjerana, Eugenia microcarpa. A an?lise de agrupamento entre as dez ?reas indicou, em geral, maior semelhan?a em decorr?ncia da proximidade entre ?reas do que pela orienta??o da encosta, entretanto comparando as vertentes norte e sul mais pr?ximas, observase que os maiores ?ndices de diversidade, a maior propor??o de esp?cies raras e exclusivas e os est?gios de sucess?o mais avan?ados foram encontradas na vertente sul. Os casos em que isso n?o ocorreu foram observados nas ?reas mais pr?ximas ao per?metro urbano que, independente da vertente apresentaram menor diversidade flor?stica. A distribui??o diam?trica apresentou modelo do J invertido, sendo a amplitude diam?trica bastante vari?vel entre ?reas. N?o foi encontrado um padr?o entre distribui??o diam?trica e a orienta??o da encosta. Comparando a flor?stica do trecho estudado do Maci?o da Pedra Branca com outras ?reas florestais do Rio de Janeiro, observou-se uma baixa similaridade flor?stica. A maior identidade flor?stica ocorreu com trabalhos realizados na pr?pria Pedra Branca, na Serra da Tiririca, e na Floresta da Tijuca. Trabalhos fora do munic?pio apresentaram similaridade flor?stica inferior a 25%. A elevada diversidade da ?rea estudada ? reflexo da sua alta heterogeneidade ambiental, relacionada a diferen?as na altitude, orienta??o de encosta, est?gio sucessional e hist?rico de uso, o que coloca a Maci?o da Pedra Branca como ?rea estrat?gica para conserva??o de esp?cies do munic?pio do Rio de Janeiro.

Made available in DSpace on 2014-12-17T14:13:58Z (GMT). No. of bitstreams: 1 KalineDM_DISSERT.pdf: 984274 bytes, checksum: 8150a2011f0eac140bc8113ffa4bc1ea (MD5) Previous issue date: 2011-12-02 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / In the present study, six families of sulfated polysaccharides were obtained from seaweed Dictyopteris delicatula (Lamouroux, 1809) and their anticoagulant, antioxidant and antitumor activities were evaluated. All fractions showed anticoagulant activity on aPTT assay, but not on PT assay. Fractions also exhibited total antioxidant activity, superoxide radical scavenging capacity and ferric chelating property. Thus, six fractions (F0.5v, F0.7v, F1.0v, F1.3v, F1.5v e F2.0v) we obtained by proteolytic digestion, followed by acetone fractionation and molecular sieving on Sephadex G-100. Chemical analyses demonstrated that all polysaccharides contain heterofucans composed mainly of fucose, xylose, glucose, galactose, uronic acid, and sulfate. Any fractions changed the PT. However, all fractions were able on double the aPPT on a dose-dependent manner. The heterofucans F0.7v and F1.0v showed low anticoagulant activity while F1.5v presented the most prominent anticoagulant activity .When compared to Clexane?, a low molecular weight heparin, at same concentration F1.5v presented similar anticoagulant activity. The fucans F0.5v and F0.7v at 1.0 mg/mL showed high ferric chelating activity (~45%), whereas fucans F1.3v (0.5 mg/mL) showed considerable reducing power, about 53.2% of the activity of vitamin C. The fucan F1.5v presented the most prominent anticoagulant activity. The best antiproliferative activity was found with fucans F1.3v and F0.7v. However, F1.3v activity was much higher than F0.7v inhibiting almost 100% of HeLa cell proliferation. These fucans have been selected for further studies on structural characterization as well as in vivo experiments, which are already in progress / No presente estudo, seis popula??es de polissacar?deos sulfatados foram obtidas a partir da alga Dictyopteris delicatula (Lamouroux, 1809) e suas atividades anticoagulante, antioxidante e antitumoral avaliadas. Deste modo, as seis fra??es (F0.5v, F0.7v, F1.0v, F1.3v, F1.5v e F2.0v) foram obtidas por prote?lise seguida por fracionamento de acetona e gel filtra??o molecular Sephadex G-100. Todas as fra??es apresentaram atividade anticoagulante frente ao ensaio de aPTT, mas n?o sobre o ensaio de PT. As heterofucanas exibiram atividade antioxidante total, capacidade em sequestrar radicais super?xido e propriedade de quelar ferro. As an?lises qu?micas demonstraram que todos os polissacar?deos cont?m heterofucanas composta principalmente por fucose, xilose, glicose, galactose, ?cido ur?nico, e sulfato. Nenhuma das fra??es alterou o PT. Entretanto, todas as fra??es foram capazes de dobrar o aPTT de uma maneira dose dependente. As heterofucanas F0.7v e F1.0v demonstraram baixa atividade anticoagulante, enquanto a F1.5v apresentou a maior atividade anticoagulante e quando comparada com Clexane?, uma heparina de baixo peso molecular, em mesma concentra??o, apresentou antividade anticoagulante semelhante. As fucanas F0.5v e F0.7v a 1,0 mg/mL mostraram alta atividade de quela??o ferica (~ 45%), enquanto a fucana F1.3v (0,5 mg/mL) mostrou consider?vel potencial redutor, cerca de 53,2% da atividade da vitamina C. A melhor atividade antitumoral foi encontrada nas fucanas F1.3v e F0.7v. No entanto, a atividade F1.3v foi muito superior a F0,7v, quase 100% de inibi??o da prolifera??o de c?lulas HeLa (c?lula de c?ncer de colo de ?tero). Estas fucanas foram selecionadas para novos estudos sobre caracteriza??o estrutural, bem como em experimentos in vivo, que j? est?o em andamento / 2020-01-01

Made available in DSpace on 2014-12-17T14:16:23Z (GMT). No. of bitstreams: 1 DeboraAN.pdf: 367004 bytes, checksum: a62b214f06755ba9b9d2aedbea46c4f9 (MD5) Previous issue date: 2006-05-24 / This study aimed builds reference values for copper and zinc, of healthy adults in Natal-RN, and to identify the influence of the gender, age, body mass index (BMI) and diet, on those values. They were assessed 123 healthy students of the Universidade Federal do Rio Grande do Norte (UFRN), both genders, with age between 19 and 41 years. The project was approved by the Ethics Committee in Research of UFRN. BMI was determined and the food consume was accomplished through a 24h recordatory. Dietary was evaluated as the energy, macronutrients, copper and zinc, according to the recommendations of National Academy of Sciences (2001; 2002). Analyses of the copper and zinc concentrations in the plasma and erythrocytes were accomplished by flame atomic absorption spectrometry. The casuistic came quite homogeneous as for the distribution for gender and age, being the largest number of individuals between the 19 and 24 years old. Most of the volunteers presented anthropometric nutritional state inside of the normality patterns. Chronic diseases family antecedents and sedentarysm were observed. Diet was characterized with low consumption of zinc, appropriate of copper and of lipids. Average concentrations of plasma copper (p=0,002), erythrocyte copper (&#956;g/dL, p=0,036; &#956;g/gHb, p=0,038), and plasma zinc (p=0,022) were different among the genders, what was demonstrated by the largest values of copper in the female gender and larger of zinc in the masculine. Plasma copper values still suffered interference of the variables: energy, carbohydrate and copper consumption, all classified in agreement with the median, besides the protein classified according to the percentage contribution for the dietary total energy. The study allowed to establish reference values for erythrocyte zinc (1.261,6-1.344,0 &#956;g/dL e 51,0-54,3 &#956;g/gHb) and to suggest "indicative" of reference values for plasma (108,4 130,2 &#956;g/dL) and erythrocyte (female = 85,0 91,4 &#956;g/dL; masculine = 80,2 86,5 &#956;g/dL) copper and plasma zinc (female = 98,8 105,8 &#956;g/dL; masculine = 104,6 111,6 &#956;g/dL) / O estudo teve como objetivo construir valores de refer?ncia para cobre e zinco, de adultos saud?veis na cidade do Natal-RN, e identificar a influ?ncia do g?nero, idade, ?ndice de massa corporal (IMC) e dieta, sobre esses valores. Foram avaliados 123 estudantes saud?veis da Universidade Federal do Rio Grande do Norte (UFRN), de ambos os g?neros, com idade entre 19 e 41 anos. O projeto foi aprovado pelo Comit? de ?tica em Pesquisa da UFRN. Foi estimado o indice de massa corporal e realizado um recordat?rio alimentar de 24h. A dieta foi avaliada quanto a energia, macronutrientes, cobre e zinco, segundo as recomenda??es da National Academy of Sciences (2001; 2002). As concentra??es de cobre e zinco no plasma e no eritr?cito foram realizadas por espectrofotometria de absor??o at?mica de chama. A casu?stica apresentou-se bastante homog?nea quanto ? distribui??o por g?nero e idade, estando o maior n?mero de indiv?duos entre os 19 e 24 anos. A maioria dos volunt?rios apresentou estado nutricional antropom?trico dentro dos padr?es de normalidade. Antecedentes familiares de doen?as cr?nicas e sedentarismo foram observados. A dieta caracterizou-se com baixo consumo de zinco, adequado de cobre e de lip?deos. As concentra??es m?dias de cobre no plasma (p=0,002), cobre no eritr?cito (&#956;g/dL, p=0,036; &#956;g/gHb, p=0,038), e zinco no plasma (p=0,022), foram estatisticamente diferentes entre os g?neros, o que foi demonstrado pelos maiores valores de cobre do g?nero feminino e maiores de zinco no masculino. Os valores de cobre no plasma sofreram ainda interfer?ncia das vari?veis: ingest?o de calorias, carboidrato e cobre na dieta, todos categorizados de acordo com a mediana, al?m da prote?na categorizada segundo o percentual de contribui??o para o valor cal?rico total (VCT) da dieta. O estudo permitiu estabelecer valores de refer?ncia para zinco no eritr?cito, correspondentes a 1.261,6-1.344,0 &#956;g/dL e 51,0-54,3 &#956;g/gHb, e sugerir indicativos de valores de refer?ncia para cobre no plasma (108,4 130,2 &#956;g/dL), no eritr?cito (feminino = 85,0 91,4 &#956;g/dL; masculino = 80,2 86,5 &#956;g/dL) e zinco no plasma (feminino 98,8 105,8 = &#956;g/dL; masculino 104,6 111,6 = &#956;g/dL)

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-05T23:00:04Z No. of bitstreams: 1 LiviaDeLourdesDeSousaPinto_DISSERT.pdf: 1563914 bytes, checksum: afff2cde0e6703ffa7b4fae03c2036ac (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-02-16T22:03:58Z (GMT) No. of bitstreams: 1 LiviaDeLourdesDeSousaPinto_DISSERT.pdf: 1563914 bytes, checksum: afff2cde0e6703ffa7b4fae03c2036ac (MD5) / Made available in DSpace on 2016-02-16T22:03:58Z (GMT). No. of bitstreams: 1 LiviaDeLourdesDeSousaPinto_DISSERT.pdf: 1563914 bytes, checksum: afff2cde0e6703ffa7b4fae03c2036ac (MD5) Previous issue date: 2015-03-13 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Condroitim sulfato (CS) ? um glicosaminoglicano natural presente na matriz extracelular de tecidos conectivos, podendo ser extra?do e purificado desses tecidos. O CS est? envolvido em diversas fun??es biol?gicas, o que pode estar relacionado ? variabilidade estrutural que possui, apesar da simplicidade da cadeia linear dessa mol?cula. Pesquisas na ?rea biotecnol?gica e farmacol?gica com rejeitos provenientes da aquicultura vem sendo desenvolvidas no Brasil. Nas ?ltimas d?cadas, a til?pia (Oreochromis niloticus), peixe nativo da ?frica, tem sido uma das esp?cies mais cultivadas em v?rias regi?es do mundo, inclusive no Brasil. A tilapicultura ? uma atividade economica cujo principal inconveniente ? a grande quantidade de res?duos que s?o descartados pelos produtores. Entende-se que o material descartado pode ser aproveitado em pesquisas como fonte de mol?culas com importante aplica??o biotecnol?gica, o que tamb?m contribui na redu??o de impactos ambientais e favorece o desenvolvimento dessa atividade de maneira ecologicamente correta. Dessa forma, v?sceras de til?pias nil?tica foram submetidas ? prote?lise, em seguida os glicosaminoglicanos foram complexados com resina de troca i?nica (Lewatit), fracionados com volumes crescentes de acetona e purificados atrav?s da cromatografia de troca i?nica DEAE-Sephacel. Na sequ?ncia, a fra??o foi analisada atrav?s de eletroforese em gel de agarose e resson?ncia magn?tica nuclear (RMN). O perfil eletrofor?tico do composto, a an?lise dos espectros 1H de RMN e a correla??o do HSQC permitem afirmar que o composto corresponde a uma mol?cula de condroitim sulfato. O ensaio de MTT foi utilizado para avalia??o da viabilidade celular na presen?a do CS isolado de til?pia e mostrou que o composto n?o ? citot?xico a c?lulas normais provenientes do fibroblasto de embri?o de camundongos (3T3). Ent?o, o composto foi testado quanto a capacidade de reduzir o influxo de leuc?citos em modelo de peritonite aguda (in vivo) induzida por tioglicolato de s?dio. Nesse contexto, foi realizada a contagem total e diferencial de leuc?citos do sangue e l?quido peritoneal coletadas, respectivamente, da veia cava e do lavado peritoneal de cada animal submetido ao experimento. O condroitim sulfato, pela primeira vez isolado de til?pia (CST ), foi capaz de reduzir a migra??o de leuc?citos ? cavidade peritoneal de camundongos inflamados em at? 80,4% na dose de 10?g/kg. Os resultados mostram ainda que houve redu??o significativa (p<0,001) da popula??o de polimorfonucleares do lavado peritoneal nas tr?s doses testadas (0,1?g/kg; 1?g/kg e 10?g/kg) quando comparado ao controle positivo (apenas tioglicolato). Portanto, uma vez que a estrutura e o mecanismo de a??o do CST tenham sido totalmente elucidados, esse composto pode apresentar potencial para uso terap?utico em doen?as inflamat?rias. / Chondroitin sulfate (CS) is a naturally glycosaminoglycan found in the extracellular matrix of connective tissues and it may be extracted and purified those tissues. CS is involved in various biological functions, which may be related to the having structural variability, despite the simplicity of the linear chain structure from this molecule. Researches in biotechnology and pharmaceutical field with wastes from aquaculture has been developed in Brazil. In recent decades, tilapia (Oreochromis niloticus), native fish from Africa, has been one of the most cultivated species in various regions of the world, including Brazil. The tilapia farming is a cost-effective activity, however, it generates large amount of wastes that are discarded by producers. It is understood that waste from tilapia can be used in research as a source of molecules with important biotechnological applications, which also helps in reducing environmental impacts and promote the development of an ecofriendly activity. Thus, nile tilapia viscera were subjected to proteolysis, then the glycosaminoglycans were complexed with ion exchange resin (Lewatit), it was fractionated with increasing volumes of acetone and purified by ion exchange chromatography DEAE-Sephacel. Further, the fraction was analyzed by agarose gel electrophoresis and nuclear magnetic resonance (NMR). The electrophoretic profile of the compound together the analysis of 1H NMR spectra and the HSQC correlation allow to affirm that the compound corresponds to a molecule like chondroitin sulfate. MTT assay was used to assess cell viability in the presence of CS tilapia isolated and showed that the compound is not cytotoxic to normal cells such as cells from the mouse embryo fibroblast (3T3). Then, this compound was tested for the ability to reduce the influx of leukocytes in model of acute peritonitis (in vivo) induced by sodium thioglycolate. In this context, it was done total and differential leukocytes counting in the blood and peritoneal fluid collected respectively from vena cava and the peritoneal cavity of the animals subjected to the experiment. The chondroitin sulfate for the first time isolated from tilapia (CST ) was able to reduce the migration of leukocytes to the peritoneal cavity of inflamed mice until 80.4 per cent at a dose 10?g/kg. The results also show that there was a significant reduction (p<0.001) of the population of polymorphonuclear leukocytes from peritoneal cavity in the three tested doses (0.1?g/kg; 1?g/kg and 10?g/kg) when it was compared to the positive control (just thioglycolate). Therefore, since the CST structure and mechanism of action has been completely elucidated, this compound may have potential for therapeutic use in inflammatory diseases