Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-10T21:38:12Z No. of bitstreams: 1 LuanaGabrielleDeFrancaFerreira_DISSERT.pdf: 3743895 bytes, checksum: 6586155eff1318ab76c3be9fd110f030 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-17T22:48:47Z (GMT) No. of bitstreams: 1 LuanaGabrielleDeFrancaFerreira_DISSERT.pdf: 3743895 bytes, checksum: 6586155eff1318ab76c3be9fd110f030 (MD5) / Made available in DSpace on 2016-03-17T22:48:47Z (GMT). No. of bitstreams: 1 LuanaGabrielleDeFrancaFerreira_DISSERT.pdf: 3743895 bytes, checksum: 6586155eff1318ab76c3be9fd110f030 (MD5) Previous issue date: 2015-04-10 / Diversos estudos revelam que nas ?ltimas d?cadas ocorreu uma diminui??o na dura??o do sono. Os compromissos sociais, como o trabalho e a escola, muitas vezes n?o est?o alinhados ao ?tempo biol?gico? dos indiv?duos. Somada a isso, observa-se uma menor for?a do zeitgeber causada pela menor exposi??o ? luz durante o dia e maior ? noite. Isso gera um d?bito cr?nico de sono que ? compensado nos dias livres, ocorrendo semanalmente uma restri??o e extens?o do sono denominada de jet lag social. A priva??o de sono vem sendo associada ? obesidade, risco cancer?geno e cardiovascular. Desta-forma, sugere-se que o sistema nervoso auton?mico seja um caminho que relaciona os problemas do sono ?s doen?as cardiovasculares. No entanto, al?m das evid?ncias demonstradas por pesquisas com uso de modelos de priva??o de sono de forma aguda e controlada, s?o necess?rios estudos investigando efeitos da priva??o do sono de forma cr?nica como ocorre no jet lag social. O objetivo deste estudo foi investigar a influ?ncia do jet lag social em marcadores circadianos de atividade-repouso e card?acos em estudantes do Curso de Medicina. Trata-se de um estudo transversal e observacional realizado no Laborat?rio de Neurobiologia e Ritmicidade Biol?gica (LNRB) do Departamento de Fisiologia da UFRN. Participaram da pesquisa estudantes de medicina matriculados no 1? per?odo do curso da UFRN. Foram utilizados os seguintes instrumentos: Question?rio cronotipo de Munique (MCTQ); Question?rio para identifica??o de indiv?duos matutinos e vespertinos (MEQ ou HO); ?ndice de qualidade do sono de Pittsburgh; Escala de Sonol?ncia de Epworth; Act?metro; Cardiofrequenc?metro. Foram analisadas vari?veis de caracteriza??o do sono, n?o param?tricas (IV60, IS60, L5 e M10) e ?ndices card?acos no dom?nio do tempo, frequ?ncia (LF, HF, LF/HF) e n?o linear (SD1, SD2, SD1/SD2). Realizou-se an?lise estat?stica descritiva, comparativa e de correla??o com uso do programa SPSS vers?o 20. Participaram do estudo 41 estudantes, 48,8% (20) mulheres e 51,2% (21) homens, com 19,63 ? 2,07 anos. O jet lag social teve uma m?dia de 02:39h ? 00:55h, 82,9% (34) com jet lag social ? 1 hora e houve correla??o negativa com escore cronotipo de Munique evidenciando maior priva??o do sono em indiv?duos com tend?ncia ? vespertinidade. Qualidade do sono ruim foi detectada em 90,2% (37) (X 2 = 26,56, p < 0,001) e 56,1% (23) sonol?ncia diurna excessiva (X 2 = 0,61, p = 0,435). Observou-se diferen?a significativa dos valores de LFnu, HFnu e LF/HF entre os grupos de jet lag social < 2h e ? 2h e houve correla??o do jet lag social com LFnu (rs = 0,354, p = 0,023), HFnu (rs = - 0,354, p = 0,023) e LF/HF (rs = 0,355, p = 0,023). Verificou-se ainda associa??o negativa entre IV60 e ?ndices no dom?nio do tempo e n?o lineares. Sugere-se que a priva??o cr?nica de sono pode ter associa??o com maior atividade simp?tica promovendo aumento no risco cardiovascular. / Studies reveal that in recent decades a decrease in sleep duration has occurred. Social commitments, such as work and school are often not aligned to the "biological time" of individuals. Added to this, there is a reduced force of zeitgeber caused by less exposure to daylight and larger exposure to evenings. This causes a chronic sleep debt that is offset in a free days. Indeed, a restriction and extent of sleep called "social Jet lag" occurs weekly. Sleep deprivation has been associated to obesity, cancer, and cardiovascular risk. It is suggested that the autonomic nervous system is a pathway that connects sleep problems to cardiovascular diseases. However, beyond the evidence demonstrated by studies using models of acute and controlled sleep deprivation, studies are needed to investigate the effects of chronic sleep deprivation as it occurs in the social jet lag. The aim of this study was to investigate the influence of social jet lag in circadian rest-activity markers and heart function in medical students. It is a cross-sectional, observational study conducted in the Laboratory of Neurobiology and Biological Rhythmicity (LNRB) at the Department of Physiology UFRN. Participated in the survey medical students enrolled in the 1st semester of their course at UFRN. Instruments for data collection: Munich Chronotype Questionnaire, Morningness Eveningness Questionnaire of Horne and ?stberg, Pittsburgh Sleep Quality Index, Epworth Sleepiness Scale, Actimeter; Heart rate monitor. Analysed were descriptive variables of sleep, nonparametric (IV60, IS60, L5 and M10) and cardiac indexes of time domain, frequency (LF, HF LF / HF) and nonlinear (SD1, SD2, SD1 / SD2). Descriptive, comparative and correlative statistical analysis was performed with SPSS software version 20. 41 students participated in the study, 48.8% (20) females and 51.2% (21) males, 19.63 ? 2.07 years. The social jet lag had an average of 02: 39h ? 00:55h, 82.9% (34) with social jet lag ? 1h and there was a negative correlation with the Munich chronotype score indicating greater sleep deprivation in subjects prone to eveningness. Poor sleep quality was detected in 90.2% (37) (X2 = 26.56, p <0.001) and 56.1% (23) excessive daytime sleepiness (X2 = 0.61, p = 0.435). Significant differences were observed in the values of LFnu, HFnu and LF / HF between the groups of social jet lag <2h and ? 2h and correlation of the social jet lag with LFnu (rs = 0.354, p = 0.023), HFnu (rs = - 0.354 , p = 0.023) and LF / HF (r = 0.355, p = 0.023). There was also a negative association between IV60 and indexes in the time domain and non-linear. It is suggested that chronic sleep deprivation may be associated with increased sympathetic activation promoting greater cardiovascular risk.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-06-06T20:36:54Z No. of bitstreams: 1 GiovannaKarinnyPereiraCruz_DISSERT.pdf: 2053427 bytes, checksum: f046a7ecc9bfbda6858a768715f96404 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-06-08T20:02:03Z (GMT) No. of bitstreams: 1 GiovannaKarinnyPereiraCruz_DISSERT.pdf: 2053427 bytes, checksum: f046a7ecc9bfbda6858a768715f96404 (MD5) / Made available in DSpace on 2016-06-08T20:02:03Z (GMT). No. of bitstreams: 1 GiovannaKarinnyPereiraCruz_DISSERT.pdf: 2053427 bytes, checksum: f046a7ecc9bfbda6858a768715f96404 (MD5) Previous issue date: 2015-10-15 / Objetivou-se caracterizar cl?nica e epidemiologicamente os pacientes em fila de espera e os transplantados com tecido corneano em um servi?o de refer?ncia para transplantes de c?rnea no estado do Rio Grande do Norte. Trata de um estudo epidemiol?gico de abordagem quantitativa, de corte transversal, descritivo e anal?tico que incluiu todos os pacientes em fila de espera para o transplante (popula??o A) e os transplantados com tecido corneano em um servi?o de refer?ncia (popula??o B). Na popula??o A foi realizado o censo dos pacientes em lista de espera para o transplante de c?rnea (n=62 pacientes). Na popula??o B a amostragem foi n?o probabil?stica e correspondeu a todos os transplantes de c?rnea realizados no servi?o no per?odo de 2010 a 2014 (n= 258). Estudo aprovado pelo Comit? de ?tica em Pesquisas da Universidade Federal do Rio Grande do Norte, sob Parecer n? 876.177 e CAAE n? 37533014.8.0000.5537. Os dados foram coletados em sua totalidade no per?odo de janeiro a abril de 2015, mediante dois instrumentos constru?dos para sistematizar a coleta dos dados necess?rios. Ap?s serem codificados e tabulados, foram analisados por meio do software Statistical Package for the Social Sciences, vers?o 20.0. A descri??o das vari?veis e seus padr?es de distribui??o foram apresentados por frequ?ncias e medidas de tend?ncia central, enquanto que para an?lise multivariada foram aplicadas medidas de magnitude de efeito (raz?o de preval?ncia) e medidas de associa??o (teste qui-quadrado ou de exato de Fisher), para um n?vel de signific?ncia de 0,05. Os resultados s?o apresentados em dois artigos cient?ficos oriundos dos dados da pesquisa de campo. Verificou-se que o perfil epidemiol?gico dos pacientes em fila de espera (n=62) apresentou preval?ncia de indiv?duos com idade superior a 50 anos, do sexo feminino (54,84%) e residentes da mesorregi?o do Leste Potiguar (66,13%). O perfil cl?nico dos pacientes submetidos ao transplante de c?rnea (n=258) foi caracterizado por serem do sexo masculino (51,16%), com idade m?dia de 49,33 anos e 57,75% provenientes do Leste Potiguar. O tempo m?dio em fila de espera foi 172,63 e 9,03 dias em transplantes eletivos e de urg?ncia, respectivamente. A principal condi??o indicadora para realiza??o do transplante foi o ceratocone. Para os pacientes em fila de espera a vari?vel ?tipo de dist?rbio da c?rnea? apresentou associa??o estatisticamente significativa com rela??o ?s vari?veis sexo e faixa et?ria, enquanto que para a amostra referente aos transplantes de c?rnea a vari?vel ?tipo de dist?rbio da c?rnea? apresentou associa??o com as vari?veis sexo, faixa et?ria, cirurgia pr?via, fal?ncia do enxerto anterior, classifica??o do olho e glaucoma. Mediante a caracteriza??o do perfil epidemiol?gico e cl?nico dos transplantes de c?rneas, torna-se poss?vel problematizar a realidade, pontuar sobre o cuidado que deve ser ofertado e desenvolver interven??es direcionadas ?s necessidades coletivas e individuais intr?nsecas aos pacientes que necessitam desse procedimento cir?rgico como op??o terap?utica. / This study aimed to characterize in a clinical and epidemiological way the patients who are on a waiting list for transplantation and the patients transplanted with corneal tissue in a corneal transplants reference service in the state of Rio Grande do Norte. It is an epidemiological study of a quantitative approach, with cross-sectional, descriptive and analytical cut including all patients on the waiting list for transplantation (population A) and the patients already transplanted with a corneal tissue (population B) in a reference service. In population A, there was a census conducted of patients on the waiting list for corneal transplantation (n=62 patients). In population B, the sample was non-probabilistic and corresponded to all corneal transplants performed in the service in the period from 2010 to 2014 (n=258). This study is approved by the Research Ethics Committee of the Federal University of Rio Grande do Norte, in Opinion 876 177 and CAAE 37533014.8.0000.5537. Data were collected in full in the period from January to April 2015, by two instruments built to systematize the necessary data collection. After being coded and tabulated, data were analyzed using the Statistical Package for Social Sciences software, version 20.0. The definition of variables and their distribution patterns were presented as frequencies and measures of central tendency while, for multivariate analysis, the effect of magnitude measures were applied (prevalence ratio) and measures of association (chi-square test or Fisher's exact test) for a 0.05 significance level. The results are shown in two scientific articles coming from the field survey data. It was found that the epidemiological profile of patients on the waiting list (n=62) showed a prevalence of individuals aged over 50 years old, female (54.84%) and residents of the middle region of East Rio Grande do Norte (66.13%). The clinical profile of patients with corneal transplantation (n=258) was characterized by being male (51.16%) with an average age of 49.33 years old and 57.75% were coming from East Rio Grande do Norte. The average time on the waiting list was 172.63 days in elective transplants and 9.03 days in urgent transplants. Keratoconus was the main indicator condition to perform the transplant. For patients on the waiting list, the variable ?type of disorder of the cornea? showed statistically significant association with gender and age. For patients with corneal transplants, the variable "type of disorder of the cornea" was associated with the variables gender, age, previous surgery, failure of previous graft, classification of the eye and glaucoma. By characterizing the clinical and epidemiological profile of corneal transplants, it is possible to question the reality, pointing about the care that should be offered and develop targeted interventions to collective and individual needs intrinsic to patients who need this surgery as a treatment option.

Made available in DSpace on 2014-12-17T15:03:24Z (GMT). No. of bitstreams: 1 RaulysonFA_DISSERT.pdf: 2784918 bytes, checksum: f9eec0ac84786bd83f2f7e5f5d700dcc (MD5) Previous issue date: 2009-11-06 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Although the good performance in organic matter and suspended solids removal, the anaerobic reactors are unable to remove ammonia nitrogen from sewage, which makes indispensable to include a step of post-treatment for removal of ammonia or nitrate as necessary. This paper presents the performance of a new variant technology, where the nitrification unit, preceded by anaerobic units, is a submerged aerated biological filter, without continuous sludge discharge in their daily operation. The oxygenation system is very simple and inexpensive, consisting of perforated hoses and compressors. The anaerobic reactors are a septic tank with two chambers followed (8.82 m?) and two parallel anaerobic filters (36 m? each) filled with ceramic bricks and conics plastic parts. Both followed aerated filters were filled with cut corrugated conduit. The study evaluated the behavior of the system with constant domestic sewage flow (10 m?/d) and different aeration conditions, are these: stage 01, when applied air flow of 0.01 m? air/min in both aerated filter; stage 02, remained in the initial air flow rate in the second aerated filter and increased at the first to 0.05 m? air/min; at last, at last, in stage 03, the air flow rate of first aerated filter was 0.10 m? air/min and on the second remained at 0.01 m? air/min. The filter FA1 received load of 0.41 kg COD/m?.d, 0.37 kg COD/m?.d and 0.26 kg COD/m?.d on phases 01, 02 and 03, respectively. The FA2 received loads of 0.25 kg COD/m?.d, 0.18 kg COD/m?.d and 0.14 kg COD/m?.d on phases 01, 02 and 03, respectively. During stage 01, were found the following results: 98% removals of BODtotal and 92% of CODtotal, with effluent presenting 9 mg/L of BODtotal final average and 53 mg/L of CODtotal average; suspended solids removals of 93%, with a mean concentration of 10 mg/L in the final effluent; 47% reduction of ammonia of FA2 to FAN 's, presenting average of 28 mg NNH3/ L of ammonia in the effluent with; the dissolved oxygen levels always remained around 2.0 mg/L. During stage 02, were found removals of 97% and 95% to BODtotal and suspended solids, respectively, with average final concentrations of 8 and 7 mg/L, respectively; was removed 60% of ammonia, whose final concentration was 16.3 mg NNH3/ L, and nitrate was increased to a final average concentration of 16.55 mg N-NO3/L. Finally, the stage 03 provided 6 mg/L of DBOtotal (98% removal) and 23 mg/L of CODtotal (95% removal) of final effluent concentrations average. At this stage was identified the higher ammonia oxidation (86%), with final effluent showing average concentration of 6.1 mg N-NH3/L, reaching a minimum of 1.70 mg N-NH3/L. In some moments, during stage 03, there was a moderate denitrification process in the last aerated filter. The average turbidity in the effluent showed around 1.5 NTU, proving the good biomass physical stability. Therefore, the results demonstrate the submerged biological filters potential, filled with high void ratio material (98%), and aerated with hoses and compressor adoption, in the carbonaceous and nitrogenous matter oxidation, also generating an effluent with low concentration of solids / Apesar de bom desempenho no tocante ? remo??o de mat?ria org?nica e s?lidos suspensos, os reatores anaer?bios s?o incapazes de remover nitrog?nio amoniacal dos esgotos, o que torna indispens?vel a inclus?o de uma etapa de p?s-tratamento para a remo??o da am?nia ou do nitrato, sempre que necess?rio. Neste trabalho ? apresentado o desempenho de uma nova variante tecnol?gica, em que a unidade de nitrifica??o, precedida por unidades anaer?bias, ? um filtro biol?gico submerso aerado, sem cont?nuas descargas de lodo em sua opera??o cotidiana. O sistema de oxigena??o ? muito simples e de baixo custo, constitu?do por mangueiras perfuradas e compressores. Os reatores anaer?bios s?o um decanto-digestor de duas c?maras em s?rie (8,82 m?) e dois filtros anaer?bios em paralelo (cada um com 3,36 m?) preenchidos com tijolo cer?mico e pe?as pl?sticas c?nicas. Os dois filtros aerados, em s?rie, foram preenchidos com eletrodutos corrugados cortados. No estudo avaliou-se o comportamento do sistema com vaz?o de esgoto dom?stico constante (10 m?/d) e diferentes condi??es de aera??o, quais sejam: fase 01, quando se aplicou vaz?o de 0,01 m? ar/min nos dois filtros aerados; fase 02, manteve-se a vaz?o inicial no segundo filtro aerado e aumentou a do primeiro para 0,05 m? ar/min; por fim, na fase 03, a vaz?o do primeiro filtro aerado foi 0,10 m? ar/min e a do segundo permaneceu ainda em 0,01 m? ar/min. O filtro FA1 recebeu carga de 0,41 kg DQO/m?.d, 0,37 kg DQO/m?.d e 0,26 kg DQO/m?.d nas fases 0,1, 02 e 03, respectivamente. O FA2 recebeu cargas de 0,25 kg DQO/m?.d, 0,18 kg DQO/m?.d e 0,14 kg DQO/m?.d nas fases 01, 02 e 03, respectivamente. Durante a fase 01, foram observados os seguintes resultados: remo??es de 98% de DBOtotal e 92% de DQOtotal, com efluente apresentando DBOtotal m?dia final de 9 mg/L e DQOtotal m?dia de 53 mg/L; remo??o em torno de 93% de s?lidos suspensos, com concentra??o m?dia de 10 mg/L no efluente final; redu??o de 47% de am?nia do FA2/FAN s, apresentando am?nia no efluente final com m?dia de 28 mg N-NH3/L; os n?veis de oxig?nio dissolvido sempre mantiveram-se em torno de 2,0 mg/L. Durante a fase 02, foram verificados para DBOtotal e s?lidos suspensos remo??es de 97% e 95%, respectivamente, com concentra??es finais m?dias de 8 e 7 mg/L, respectivamente; o nitrog?nio amoniacal foi removido em 60%, cuja concentra??o final foi de 16,3 mg N-NH3/L, e o nitrato foi incrementado para uma concentra??o m?dia final de 16,55 mg N-NO3 -/L. Por fim, a fase 03 proporcionou efluente com concentra??es finais m?dias de 6 mg/L de DBOtotal (remo??o de 98%) e 23 mg/L de DQOtotal (remo??o de 95%). Nesta fase foi identificada a maior oxida??o de nitrog?nio amoniacal (86%), com efluente final apresentando concentra??o m?dia de 6,1 mg NNH3/ L, chegando a alcan?ar m?nimo de 1,70 mg N-NH3/L. Em alguns momentos, na fase 03, verificou-se um moderado processo de desnitrifica??o no ?ltimo filtro aerado. A turbidez m?dia no efluente final se mostrou da ordem de 1,5 NTU, comprovando a boa estabilidade f?sica da biomassa. Portanto, os resultados demonstram o potencial dos filtros biol?gicos submersos, preenchidos com material de elevado ?ndice de vazios (98%), e aerados com uso de mangueiras e compressor, na oxida??o de mat?ria carbon?cea e nitrogenada, gerando tamb?m um efluente com baixa concentra??o de s?lidos

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-01-03T19:56:08Z No. of bitstreams: 1 CinthyaMontenegroDeVasconcelosSilva_DISSERT.pdf: 1465896 bytes, checksum: 0fbac94f7a38a84105e55f2ee7340731 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-01-05T22:10:42Z (GMT) No. of bitstreams: 1 CinthyaMontenegroDeVasconcelosSilva_DISSERT.pdf: 1465896 bytes, checksum: 0fbac94f7a38a84105e55f2ee7340731 (MD5) / Made available in DSpace on 2017-01-05T22:10:42Z (GMT). No. of bitstreams: 1 CinthyaMontenegroDeVasconcelosSilva_DISSERT.pdf: 1465896 bytes, checksum: 0fbac94f7a38a84105e55f2ee7340731 (MD5) Previous issue date: 2016-05-13 / A doen?a de Parkinson (DP) ? uma desordem neurodegenerativa e progressiva com espectro cl?nico variado. Al?m dos sintomas motores cl?ssicos tamb?m podem surgir complica??es n?o-motoras, destacando-se a? problemas cognitivos, psiqui?tricos e auton?micos. Evid?ncias demonstram que tais sintomas n?o-motores frequentemente precedem o aparecimento dos sinais motores e s?o extremamente relevantes, dado o impacto negativo que causam na qualidade de vida dos indiv?duos. Os sintomas n?o-motores podem apresentar m?ltiplas causas, dentre as quais uma poss?vel disfun??o do sistema circadiano. Dessa forma, diversos processos fisiol?gicos influenciados pelo sistema de temporiza??o circadiano (STC), como o ciclo sono/vig?lia podem se mostrar alterados em pacientes acometidos pela DP. O STC ? respons?vel pela gera??o e manuten??o dos ritmos circadianos, que s?o oscila??es end?genas manifestadas pelos seres vivos em diversos processos fisiol?gicos e comportamentais, com per?odo em torno de 24 horas. Assim, ? fundamental a compreens?o dos efeitos da progress?o do processo patog?nico da DP sobre o perfil circadiano do ciclo sono/vig?lia e tamb?m de componentes do STC, em particular no n?cleo supraquiasm?tico (NSQ), o principal marcapasso do sistema. No presente estudo, ratos wistar jovens (6 meses) e de meia-idade (10 meses) foram submetidos a um modelo animal cr?nico de DP com administra??o de reserpina durante 20 dias. Ao longo do tratamento foram realizadas an?lises comportamentais do sono, bem como a avalia??o motora dos animais. Ap?s o fim do tratamento, foram realizadas an?lises imunoistoqu?micas no NSQ dos animais. Nossos resultados mostraram que o tratamento cr?nico com reserpina promoveu comprometimento motor progressivo tanto nos animais jovens quanto nos de meia-idade. Al?m disso, as an?lises comportamentais revelaram perturba??es no ciclo sono/vig?lia dos animais tratados em compara??o aos indiv?duos controle, incluindo avan?o na fase de sono e aumento na fragmenta??o do sono. As an?lises imunistoqu?micas n?o permitiram observar efeitos significativos do tratamento com reserpina sobre a composi??o neuroqu?mica do NSQ, contudo novos estudos s?o necess?rios para a avalia??o neuroqu?mica e morfom?trica desse importante marcapasso circadiano na DP. / Parkinson?s disease (PD) is a neurodegenerative and progressive disorder with varied clinical spectrum. In addition to the classic motor symptoms may also emerge non-motor complications, highlighting cognitive, psychiatric and autonomic problems. Evidence shows that such non-motor symptoms often precede the onset of motor signs and are extremely relevant given the negative impact they have on the quality of life of the individuals. Non-motor symptoms may present multiple causes, among which a possible dysfunction of the circadian system. Therefore, many physiological processes influenced by the circadian timing system (CTS) as the sleep / wake cycle can show alterations in PD patients. In this study we sought to evaluate the profile of sleep/wake behavior in a chronic model of PD in rats compared with possible neurochemical changes in the suprachiasmatic nucleus(SCN), the main pacemaker of the CTS. To this end, young (6 months) and middle-age (10 months) wistar rats were subjected to a treatment with repeated administration of reserpine (0.1 mg / kg) for 20 days. During treatment sleep behavioral analysis were performed as well as the motor assessment of the individuals. After the end of treatment, immunohistochemical analyzes were performed in the SCN of the animals. Our results showed that chronic treatment with reserpine promoted progressive motor impairment both in young as in middle-age animals. It is noticed significant losses from the 12th day of treatment. Furthermore, the behavioral analyzes revealed disturbances in sleep / wake cycle of the treated animals compared to control subjects, including advanced sleep phase and increased sleep fragmentation. Such changes were observed from the 6th day of treatment, prior to the onset of motor symptoms. The immunohistochemical analysis not allowed to observe significant effects of treatment with reserpine on the neurochemical composition of the SCN. Thus, our data support the observation that non-motor symptoms precede the onset of motor symptoms in PD and are extremely important for early clinical diagnosis of the disease.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-03T22:03:33Z No. of bitstreams: 1 ThamiresCasagrande_DISSERT.pdf: 5017698 bytes, checksum: d1b39db5bf0fea4e08ace5ef9a725ffc (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-10T21:03:13Z (GMT) No. of bitstreams: 1 ThamiresCasagrande_DISSERT.pdf: 5017698 bytes, checksum: d1b39db5bf0fea4e08ace5ef9a725ffc (MD5) / Made available in DSpace on 2017-04-10T21:03:13Z (GMT). No. of bitstreams: 1 ThamiresCasagrande_DISSERT.pdf: 5017698 bytes, checksum: d1b39db5bf0fea4e08ace5ef9a725ffc (MD5) Previous issue date: 2016-04-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A exist?ncia de um padr?o repetitivo peri?dico de atividade vocal de baleias jubarte ao longo da temporada reprodutiva pode apoiar a hip?tese da exist?ncia de um ritmo biol?gico end?geno, ou ainda, elucidar como os zeitgebers bi?ticos ou abi?ticos poderiam modular a express?o do comportamento vocal desses animais, que ? t?o importante para o sucesso reprodutivo dos indiv?duos e manuten??o do tamanho e da sa?de da popula??o. Muitos estudos sobre atividade vocal de baleias jubarte tem investigado as altera??es no padr?o temporal e geogr?fico das can??es dos machos. Tal conhecimento pode contribuir para o manejo, elucidando como a express?o comportamental pode ser modulada sob a a??o dos fatores externos sobre o meio em que indiv?duo esteja inserido. Este conhecimento fornece, juntamente com outras informa??es sobre a hist?ria de vida, biologia e ecologia da esp?cie, subs?dios para a elabora??o de medidas de conserva??o que sejam mais bem-sucedidas. O objetivo desse trabalho foi verificar a exist?ncia de ritmo na ocorr?ncia de displays vocais de machos de baleia jubarte no entorno do Parque Nacional Marinho dos Abrolhos e se a presen?a de ru?do de barco afeta a atividade vocal. Para isso, foram utilizados dados ac?sticos anteriormente coletados no entorno do parque durante os anos de 2003, 2004 e 2005. Em 2005 o esfor?o foi dividido entre uma ?rea tratamento, similar aos anos anteriores e onde passam barcos de turismo frequentemente e uma ?rea controle com pouco tr?fego de barcos. As grava??es foram visual e auralmente inspecionadas atrav?s da ferramenta para MATLAB, XBAT, a cada 2 minutos e categorizadas como: presen?a ou aus?ncia de vocaliza??es de baleias jubarte e tamb?m de ru?dos de embarca??es. Foram realizadas an?lises espectrais, cosinor e teste de correla??o atrav?s de uma rotina elaborada em MATLAB. Nossos resultados mostram a exist?ncia de um padr?o de atividade vocal para todos os anos mas ausente na ?rea controle em 2005. Uma maior atividade vocal foi observada entre os hor?rios de 15:00 da tarde e 10:00 da manh?. Por?m o mesmo padr?o n?o p?de ser observado para todos os meses amostrados da ?rea controle de 2005, na qual o tr?fego de embarca??es era bem reduzido. Quando analisado o padr?o temporal das embarca??es, o maior n?mero de ru?dos se encontravam entre as 10:00 e 15:00 revelando uma correla??o negativa entre o ru?do de embarca??es e a atividade vocal. O que pode sinalizar que devido ao ru?do dos motores das embarca??es mascarar energeticamente os sinais vocais baleias jubarte em resposta evitam vocalizar em hor?rios de maior intensidade sonora, organizando-se temporalmente. / Many studies of vocal activity of humpback whales have focused on investigating the temporal and geographical pattern changes in the male song. This knowledge can contribute to the species management, explaining how the behavioral traits are modulated by external factors of the environment in which the individual is inserted. This knowledge associated with information about the life history, biology and ecology of the species, provides the development of more successful conservation measures. Determining the existence of a periodic pattern of vocal activity of male humpback whales along the breeding season may support the hypothesis of endogenous biological rhythms, or how the biotic or abiotic zeitgebers could modulate the expression of the vocal behavior of these animals, which is so important for their reproductive success and maintainance of a increasing and healthy population. The aim of this study was to verify the existence of such rhythm in the occurrence of male vocal displays in the vicinity of the National Marine Park of Abrolhos. For this, we used acoustic data previously collected during the years 2003, 2004 and 2005. In 2005 the effort was divided into two areas, a treatment area where whale watching boat traffic is frequent similar located in the same general area to the previous years and a control area with rare boat traffic. The recordings were visually and aurally inspected every 2 minutes using XBAT an application that runs in MATLAB. These 2-minute samples were categorized as: presence or absence of humpback vocal activity and also vessel noise. Spectral analyzes were performed using Cosinor and a correlation test through a routine developed in MATLAB. Our results show the existence of a vocal activity pattern for all years in the treatment area, but not in the control area. Vocal activity concentrated between 15:00 pm and 10:00 am. The same pattern was not observed in all months of 2005 in the control area where the boat traffic is reduced. Coincidentally, the temporal pattern of vessel noise was highest between 10:00am and 15:00pm revealing a negative correlation with male vocal displays. Motorboat noise not only mask humpback male vocal displays but also the endogenous rhythm of vocal activity of humpback whales. Because of the masking caused by motorboat noise in male vocal displays, they avoid higher sound intensity schedules, organizing themselves in time.

Made available in DSpace on 2016-04-26T19:39:29Z (GMT). No. of bitstreams: 1 2007- Liamara Perin.pdf: 5126010 bytes, checksum: 0973e83f604e8d397ec95ef00c19da99 (MD5) Previous issue date: 2007-02-15 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The sugarcane crop in Brazil occupies almost six million hectares and it is economically important for the generation of jobs and energy production. Among the Poaceas species, it is the crop that receives more contributions from the biological nitrogen fixation, however until this moment it is unknown what organisms are responsible for the best results observed. Innumerable genuses of diazotrophic bacteria were found in association with sugarcane, among them the genus Burkholderia. However little is known about this genus in association with sugarcane. The objective of this study was to isolate and to characterize diazotrophic Burkholderia bacteria in association with sugarcane, and to describe a new species of diazotrophic Burkholderia bacteria. For that, samples of plants were collected in four crop areas, in different Brazilian states, for counting and isolating the bacteria community using the culture medias LGI and JMV. The isolates were characterized physiological, morphologically and molecularly. Sixty-four Burkholderia diazotrophic isolates were gotten that presented versatile metabolism, and the majority differing from the analyzed species patterns. Only five isolates were identified by the ARDRA technique, two belonging to B. tropica species and three to B. unamae. A big group, with 32 isolates, presented the same restriction profile by the ARDRA technique and differed from the analyzed species. The sequencing of the 16S rDNA region of two of these isolates showed that they don t belong to the already described species. This group, from sugarcane plants together with similar maize and pineapple isolates, was described as a new species named B. silvatlantica. The characterization of this new Burkholderia specie was confirmed by the hybridization DNA: DNA technique, presenting only 30% of similarity with the closest species, and the sequencing of the 16S rDNA region. B. Silvatlantica fixed nitrogen in microaerophilic conditions, and it had no capacity of inorganic phosphate solubilization, it did not produce vegetal hormones and had no nodulation capacity in leguminous. These results confirmed that Burkholderia is a rich genus in diazotrophics species and colonize different habitats. / A cultura da cana-de-a??car no Brasil ocupa mais de seis milh?es de hectares sendo importante economicamente pela gera??o de empregos e bastante promissora para a produ??o de energia. Dentre as Poaceas (gram?neas), ? a cultura que mais recebe contribui??es da fixa??o biol?gica de nitrog?nio, por?m ainda n?o se sabe ou n?o se conhece qual ou quais organismos s?o respons?veis pelos melhores resultados observados. In?meros g?neros de bact?rias diazotr?ficas foram encontrados em associa??o com cana-de-a??car, dentre eles o g?nero Burkholderia, at? o momento pouco estudado em associa??o com esta cultura. O objetivo deste estudo foi isolar e caracterizar bact?rias diazotr?ficas do g?nero Burkholderia em associa??o com cana-de-a??car e descrever uma nova esp?cie de bact?ria diazotr?fica do g?nero Burkholderia. Para tal, foram coletadas amostras de plantas em canaviais de quatro estados brasileiros para contagem e isolamento da comunidade de bact?rias utilizando os meios de cultura LGI e JMV. Os isolados foram caracterizados fisiologicamente, morfologicamente e molecularmente. Foram obtidos 64 isolados diazotr?ficos do g?nero Burkholderia, que apresentaram metabolismo vers?til e diferiram dos padr?es das esp?cies analisadas. Apenas cinco isolados foram identificados pela t?cnica de ARDRA, dois pertenceram a esp?cie B. tropica e tr?s a B. unamae. Um grande grupo, com 32 isolados, apresentou mesmo perfil de restri??o pela t?cnica de ARDRA e diferiu das esp?cies analisadas. O sequ?nciamento da regi?o 16S rDNA de dois destes isolados mostrou que eles n?o pertencem ?s esp?cies j? descritas. Este grupo obtido de plantas de cana-de-a??car, juntamente com isolados similares, obtidos de milho e abacaxi, foi descrito como uma nova esp?cie de nome B. silvatlantica. A nova esp?cie de Burkholderia foi confirmada pelos experimentos de hibridiza??o DNA:DNA, com apenas 30% de similaridade com a esp?cie mais pr?xima. B. silvatlantica fixou nitrog?nio em condi??es microaerof?licas, n?o apresentou capacidade de solubiliza??o de fosfato inorg?nico, n?o produziu horm?nios vegetais e n?o apresentou capacidade de nodula??o em leguminosas. Estes resultados confirmaram que o g?nero Burkholderia ? rico em esp?cies diazotr?ficas e est?o presentes em diferentes habitats.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-06-07T14:26:09Z No. of bitstreams: 1 2015 - Tassia Torres Furtado.pdf: 14895763 bytes, checksum: 7d611b6398d83fa875abc58ca88acab3 (MD5) / Made available in DSpace on 2017-06-07T14:26:09Z (GMT). No. of bitstreams: 1 2015 - Tassia Torres Furtado.pdf: 14895763 bytes, checksum: 7d611b6398d83fa875abc58ca88acab3 (MD5) Previous issue date: 2015-02-27 / The ascomycete yeast C.guttulatus, has long been recognized as a component of the normal microflora of rabbits and other herbivores. More recently, a possible association between this yeast and gastro-intestinal illness in dogs has been reported by researchers in Europe, EUA, Japan and Brazil. The current study combined morpho-phenotypic and molecular (PCR-RFLP and nucleotide sequencing), methods to examine C.guttulatus cultures recovered from Brazilian rabbits (15 isolates) and dogs (7 isolates), with the objectives of developing a means for the differential identification of C. guttulatus and to improve existing knowledge of the biology of this organism. Microscopic and macroscopic examination of isolated colonies in combination with zymogram analysis (carbohydrate fermentation tests), was unable to provide a definitive identification for the suspect cultures. In silico restriction fragment length polymorphism (RFLP) analysis of virtual PCR amplicons (corresponding to the D1-D2 domain of the gene encoding 26S ribosomal RNA of C.guttulatus and other yeast species known to be associated with dogs), indicated that digestion with the restriction enzymes DdeI, HaeIII and MspI would allow the differential identification of C. guttulatus. In vitro restriction analysis of PCR amplicons confirmed the predictions of the in silico analysis and demonstrated that differential identification of C.guttulatus could be a made using only two enzymes (DdeI and MspI). Sequencing of D1-D2 amplicons demonstrated the presence of substantial nucleotide variation within the cultures examined and divided them into three groups, denominated sequence types (ST?s). Two of these groups were closely related to each other. The third sequence type showed extensive nucleotide variation and may represent a novel species or subspecies within the genus Cyniclomyces / A levedura, Cyniclomycesguttulatus ? um ascomiceto e tem sido reconhecida como um componente da microbiota natural de coelhos e outros herb?voros. Mais recentemente, uma poss?vel associa??o entre esta levedura e doen?as gastrintestinais em c?es tem sido relatada por pesquisadores na Europa, EUA, Jap?o e Brasil. O presente estudo combinou m?todos de an?lises morfol?gica e molecular (PCR-RFLP e sequenciamento de nucleot?deos), para examinar culturas de C. guttulatus recuperados de coelhos brasileiros (15 isolados) e c?es (7 isolados), com o objetivo de desenvolvimento de um m?todo para a identifica??o diferencial de C. guttulatus e melhorar o conhecimento existente sobre a biologia desse organismo. O exame microsc?pico e macrosc?pico das col?nias isoladas em combina??o com an?lise do zimograma (testes de fermenta??o de carboidratos), n?o foi capaz de fornecer uma identifica??o definitiva para as culturas suspeitas. As an?lises in silico de polimorfismo de comprimento de fragmentos de restri??o (RFLP) de produtos de PCR virtuais (correspondentes ao dom?nio D1/D2 do gene 26S RNA ribossomal de C.guttulatus e outras esp?cies de levedura conhecidas como associadas a c?es), indicou que a digest?o com a enzimas de restri??o Ddel, HaeIII e MspI permitiria a identifica??o diferencial de C. guttulatus. As an?lises das digest?es in vitro de produtos de PCR confirmaram as previs?es das an?lises in silico e demonstrou que a identifica??o diferencial de C.guttulatus poderia ser feita usando apenas duas enzimas (DdeI e MspI). O sequenciamento dos produtos de PCR do dom?nio D1/D2 demonstraram a presen?a de varia??es substanciais de nucleot?deos nas culturas analisadas e estas foram divididas em tr?s grupos, denominados de sequ?ncias tipos (ST's). Dois destes grupos estavam estreitamente relacionados uns aos outros. O terceiro tipo de sequ?ncia mostrou grande varia??o de nucleot?deos e pode representar uma nova esp?cie ou subesp?cie dentro do g?nero Cyniclomyces

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-20T19:46:54Z No. of bitstreams: 1 2014 - Farley Alexandre da Fonseca Breda.pdf: 375196 bytes, checksum: 7001327c707e778b8396891ebff2285d (MD5) / Made available in DSpace on 2017-06-20T19:46:54Z (GMT). No. of bitstreams: 1 2014 - Farley Alexandre da Fonseca Breda.pdf: 375196 bytes, checksum: 7001327c707e778b8396891ebff2285d (MD5) Previous issue date: 2014-02-24 / CAPES / Currently with the constant demand for increased productivity and successive increasing in the prices of fertilizers, especially nitrogen, new technologies are being studied. One that has been highlighted due to low cost of deployment and promising results is the biological nitrogen fixation in grasses. The aim of this work was to study the effect of inoculation of diazotrophic bacteria Herbaspirillum seropedicae strain ZAE94 in two maize genotypes selected for the climatic conditions and low fertility soils, and the ability to reduce the use of nitrogen fertilizers, as well as increasing crop productivity. To this end, two experiments were implanted, one during the off-season and another in the harvesting of agricultural year 2012/2013. In both experiments the maize hybrids (BRS 1030 and BRS 1060) were inoculated with the ZAE94 strain, following the experimental design of a randomized block design with a factorial 2 x 2 x 3, comprising the inoculation, genotype and fertilization respectively, with six replicates. The variables analyzed were the average yield, weight of 1000 grains, content and accumulation of nitrogen in the grain and nitrogen use efficiency of fertilizer. The results were tested for normality and homogeneity, were subjected to analysis of variance, and when significant treatments, these were subjected to medium testing, using test t at 10%. For all variables the hybrid BRS 1030 was greater than the BRS 1060. For the inoculation factor and in the off-season, it was observed a triple interaction for weight of 1,000 grains; and when the inoculation is associated with the highest nitrogen rate it promoted an increase in the productivity of the hybrid BRS 1060. In harvesting period, regardless of genotype and fertilizer used, it was observed that inoculating the seed of hybrids was always better than without inoculation. For efficiency of nitrogen fertilizer usage it was observed that, in general, the BRS 1030 was more efficient than the BRS 1060. / Atualmente com a constante demanda pelo aumento de produtividade e os sucessivos aumentos nos pre?os dos fertilizantes, principalmente os nitrogenados, novas tecnologias vem sendo estudadas. Uma que vem se destacando devido ao seu baixo custo de implanta??o e os seus promissores resultados ? a fixa??o biol?gica de nitrog?nio em gram?neas. O objetivo dessa disserta??o foi estudar o efeito da inocula??o da bact?ria diazotr?fica Herbaspirillum seropedicae estirpe ZAE94, em dois gen?tipos de milho selecionados devido sua adapta??o a condi??es clim?ticas e solos de baixa fertilidade, e quanto ? capacidade de reduzir o uso de fertilizantes nitrogenados, bem como, incrementar a produtividade da cultura. Para tal, foram implantados dois experimentos um no per?odo da safrinha e outro na safra do ano agr?cola 2012/2013. Em ambos os experimentos os h?bridos de milho (BRS 1030 e BRS 1060) foram inoculados com a estirpe ZAE94, seguindo o delineamento experimental em blocos ao acaso com o fatorial de 2 x 2 x 3, sendo os fatores inocula??o, gen?tipo e aduba??o respectivamente, com 6 repeti??es. As vari?veis analisadas foram produtividade m?dia, peso de 1.000 gr?os, teor e ac?mulo de nitrog?nio nos gr?os e efici?ncia do uso do nitrog?nio fertilizante. Os resultados foram testados quanto a normalidade e homogeneidade, logo ap?s submetidos ? analise de vari?ncia e quando significativo, os mesmos foram submetidos ao teste de media. O teste de m?dia utilizado foi o teste t, a 10%. Para todas as vari?veis analisadas o h?brido BRS 1030, foi superior a BRS 1060. Para o fator inocula??o, no per?odo da safrinha, foi observada uma intera??o tripla para o peso de 1.000 gr?os, onde quando a inocula??o foi associada a maior dose de nitrog?nio ela promoveu um aumento na produtividade do h?brido BRS 1060. No per?odo da safra, independente do gen?tipo e da aduba??o utilizada observou-se que inocular a semente dos h?bridos e sempre melhor do que n?o realizar a inocula??o. Para efici?ncia do uso do nitrog?nio fertilizante foi observado que, de forma geral, o BRS 1030 foi mais eficiente do que o BRS 1060.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-07-07T13:07:27Z No. of bitstreams: 1 2010 - Fernanda Delgado Almeida.pdf: 626850 bytes, checksum: 3a7edfb868e6c7a04004c0ce79c36a91 (MD5) / Made available in DSpace on 2017-07-07T13:07:27Z (GMT). No. of bitstreams: 1 2010 - Fernanda Delgado Almeida.pdf: 626850 bytes, checksum: 3a7edfb868e6c7a04004c0ce79c36a91 (MD5) Previous issue date: 2010-02-11 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Seeds with high molybdenum (Mo) concentration can provide amounts of the nutrient highly enough to guarantee an adequate plant growth. An essay and two experiments were carried out in pots to evaluate the effect of Mo concentration in seeds of common bean (Phaseolus vulgaris L.) on the assimilation of N from the soil and the biological N2 fixation (BNF), through measuring the activities of the nitrogenase and nitrate reductase enzymes and the contribution of the BNF by the 15N isotope dilution, at different plant growth stages. The substrate was A horizon of Argisol (Hapludult) in 10 kg pots. Seeds of the cultivar Carioca enriched or not with Mo were tested. The essay had a 2x3 factorial design: two seed Mo concentrations (low and high) and three N sources (without N, inoculated and mineral N), with two replicates. Plants were harvested at 42 days after emergence (DAE). High concentration of Mo in seeds increased shoot dry mass, the nitrate reductase activity and shoot N accumulation, but reduced nodule number. The experiment 1 had a 2x2x4 factorial design: two seed Mo concentrations (low and high), two N sources (inoculated and mineral N) and four dates of harvest (20, 34, 45 and 55 DAE), with five replicates. An extra treatment was included, with seeds with high Mo concentration, inoculation and Mo added to the soil, with four harvests. No effect of Mo added to the soil was identified, as compared to the high seed Mo. High seed Mo increased the leaf area and shoot dry mass at the four times of harvest, and the pod mass at 55 DAE, at both N sources. Seeds with high Mo concentration increased the nitrate reductase activity at 45 DAE under mineral N, and nitrogenase activity at 20 and 45 DAE under inoculation. High Mo seeds increased shoot N accumulation at both N sources at pod filling stage. The experiment 2 had a 2x2x2 factorial design: two seed Mo concentrations (low and high), two N sources (inoculated and mineral N) and two dates of harvest (38 and 51 DAE), with five replicates. The soil was previously fertilized with 15N-enriched urea. Non-nodulating bean, sunflower and sorghum were used as control plants. At 51 DAE, plants originating from high Mo seeds had higher shoot mass at both N sources, but lower nodule number under mineral N. High Mo seed increased nitrate reductase activity at 51 DAE at both N sources, and nitrogenase activity at 38 DAE in inoculated plants. High Mo seeds increased shoot N accumulation at both N sources. High Mo seeds improved the contribution of the BNF at the pod filling stage, as estimated by the 15N isotope dilution technique. It is concluded that common bean seeds enriched with Mo stimulate the assimilation of N from the soil and the BNF, increasing the accumulation of biomass / Sementes com elevadas concentra??es de molibd?nio (Mo) podem fornecer quantidades do nutriente suficientes para garantir adequado crescimento ?s plantas. Foram conduzidos um ensaio e dois experimentos com objetivo de avaliar o efeito do teor de Mo em sementes de feijoeiro (Phaseolus vulgaris L.) na assimila??o do N do solo e na fixa??o biol?gica do N2 (FBN), atrav?s da mensura??o das atividades das enzimas nitrogenase e nitrato redutase, e da contribui??o da FBN pela dilui??o isot?pica de 15N, em diferentes est?dios de desenvolvimento vegetal. O substrato foi horizonte A de Argissolo em vasos de 10 kg, utilizando-se sementes da cultivar Carioca enriquecidas ou n?o com Mo. O ensaio teve arranjo fatorial 2x3: dois teores de Mo na semente (baixo e alto) e tr?s fontes de N (sem N, inoculado e N mineral), com duas repeti??es e coleta aos 42 dias ap?s emerg?ncia (DAE). O alto Mo da semente aumentou a atividade da nitrato redutase, a massa e a acumula??o de N na parte a?rea, mas reduziu o n?mero de n?dulos. O experimento 1 teve arranjo fatorial 2x2x4: dois teores de Mo na semente (baixo e alto), duas fontes de N (inoculado e N mineral) e quatro ?pocas de coleta (20, 34, 45 e 55 DAE), com cinco repeti??es. Foi inclu?do um tratamento extra, com sementes com alto Mo, inocula??o e adi??o de Mo ao solo. N?o foi identificado efeito do Mo adicionado ao solo quando comparado com o alto Mo na semente. O alto Mo na semente aumentou a ?rea foliar e a massa de parte a?rea nas quatro ?pocas de coleta, e a massa de vagem aos 55 DAE, nas duas fontes de N. Sementes com alto Mo aumentaram a atividade da nitrato redutase aos 45 DAE sob N mineral, e a atividade da nitrogenase aos 20 e 45 DAE sob inocula??o. Sementes com alto Mo aumentaram a acumula??o de N na parte a?rea nas duas fontes de N no est?dio de enchimento das vagens. O experimento 2 teve arranjo fatorial 2x2x2: dois teores de Mo na semente (alto e baixo), duas fontes de N (inoculado e N mineral) e duas coletas (38 e 51 DAE), com cinco repeti??es. O solo foi previamente adubado com ur?ia enriquecida com 15N. O feij?o n?o nodulante, girassol e sorgo serviram como plantas controle. Aos 51 DAE, plantas oriundas de sementes com alto Mo tiveram maior massa de parte a?rea nas duas fontes de N, mas menor n?mero de n?dulos sob N mineral. O alto Mo nas sementes aumentou a atividade da nitrato redutase aos 51 DAE, nas duas fontes de N, e a atividade da nitrogenase aos 38 DAE nas plantas inoculadas. Sementes com alto Mo aumentaram a acumula??o de N na parte a?rea nas duas fontes de N. Sementes com alto Mo aumentaram a contribui??o da FBN no est?dio de forma??o das vagens. Conclui-se que sementes de feijoeiro enriquecidas com Mo estimulam tanto a assimila??o do N do solo quanto a FBN, aumentando a acumula??o de biomassa e de N do feijoeiro.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-27T22:26:12Z No. of bitstreams: 1 DISSERTA??O Val?ria Silva G?es.pdf: 1629398 bytes, checksum: 6e130737f6d57007b90ea3ae8de5042b (MD5) / Made available in DSpace on 2016-09-27T22:26:12Z (GMT). No. of bitstreams: 1 DISSERTA??O Val?ria Silva G?es.pdf: 1629398 bytes, checksum: 6e130737f6d57007b90ea3ae8de5042b (MD5) Previous issue date: 2015-03-31 / The Lamiaceae family has a great importance in the Brazilian semiarid region due to their species of pharmacological properties. Martianthus leucocephalus (Mart. Ex Benth.) JFB Pastore belongs to the Lamiaceae family and has had the antimicrobial activity of essential oil proven. However, this species lacks pharmacological studies that evaluate its biological potential. Thus, the aim of this study was to evaluate the effect on the central nervous system, the acute toxicity, as well as investigate the antinociceptive and anti-inflammatory of Martianthus leucocephalus extract. The possible mechanism of action of species was also studied. The experiments were performed in the Chemistry Laboratory of Bioactive Natural Products and in the Laboratory of Pharmacology at the State University of Feira de Santana. The effect on the central nervous system was evaluated through Behavioral Pharmacological Screening by Rota Rod test. The acute toxicity was determined using the protocol Organization for Economic Cooperation and Development (OECD - 420/2001). The antinociceptive activity was investigated in the tests of acetic acid, formalin and hot plate. The mechanism of action was determined using the opioid antagonist naloxone in the hot plate model. The study of anti-inflammatory activity was performed using the paw edema induced by carrageenan tests and capillary permeability induced by acetic acid. The results were analyzed by analysis of variance (ANOVA). Martianthus leucocephalus showed no acute toxicity or muscle relaxant effects action on the motor system. The antinociceptive and anti-inflammatory properties were found significantly compared to the control group animals. / A fam?lia Lamiaceae tem uma grande import?ncia na regi?o do semi?rido brasileiro devido ?s suas esp?cies detentoras de propriedades farmacol?gicas. Martianthus leucocephalus (Mart. ex Benth.) J.F.B. Pastore pertence ? fam?lia Lamiaceae e j? teve a atividade antimicrobiana do seu ?leo essencial comprovada. No entanto, a referida esp?cie carece outros de estudos farmacol?gicos que avaliem seu potencial biol?gico. Diante disso, o objetivo desse trabalho foi avaliar o efeito sobre o sistema nervoso central, a toxidade aguda, bem como investigar as atividades antinociceptiva e anti-inflamat?ria de extratos de Martianthus leucocephalus proveniente de cultivo. O poss?vel mecanismo de a??o da esp?cie tamb?m foi estudado. Os experimentos foram realizados no Laborat?rio de Qu?mica de Produtos Naturais e Bioativos e no Laborat?rio de Farmacologia da Universidade Estadual de Feira de Santana. O efeito sobre o sistema nervoso central foi avaliado por meio da Triagem Farmacol?gica Comportamental e pelo teste do Rota Rod. A toxidade aguda foi averiguada utilizando o protocolo Organization for Economic Cooperation and Development (OECD ? 420/2001). A atividade antinociceptiva foi investigada nos testes do ?cido ac?tico, formalina e placa quente. O mecanismo de a??o foi apurado utilizando o antagonista opioide naloxona no modelo da placa quente. O estudo da atividade anti-inflamat?ria foi realizado utilizando os testes do edema de pata induzido por carragenina e da permeabilidade capilar induzida por ?cido ac?tico. Os resultados obtidos foram analisados atrav?s da an?lise de vari?ncia (ANOVA). Martianthus leucocephalus n?o mostrou toxidade aguda nem a??o miorrelaxante sobre o sistema motor. As propriedades antinociceptiva e anti-inflamat?ria foram evidenciadas de forma significativa em rela??o aos animais do grupo controle.