The subsidized island biogeography hypothesis proposes that nutrient subsidies, those translocated from one ecosystem to another, can indirectly influence species richness on islands by directly increasing terrestrial productivity. However, the lack of a formal statistical model makes it difficult to assess the strength of the hypothesis. I created a formal subsidized island biogeography model to determine how nutrient subsidies, in addition to area and distance from mainland, influence tree species richness. My model showed that an increase in terrestrial nitrogen abundance results in a decrease of tree species richness. Soil and plant δ 15N values were higher than expected and it is likely that nutrient subsidies from the marine environment are responsible for 15N enrichment. However, the range of observed nitrogen abundance is similar to inland coastal-zone forests, indicating that islands are similarly nitrogen deprived and may not be receiving enough nutrient subsidies to alter productivity. Tree species decline may therefore be more strongly related to the environmental conditions leading to patterns of nitrogen abundance rather than the abundance of nitrogen itself. Additionally, I proposed that bald eagles (Haliaeetus leucocephalus) are vectors of nutrient subsidies, depositing nutrient-rich guano at nest sites, which could alter soil chemistry and vegetation composition. In an exploratory study of seven nest sites, I found higher soil phosphorous at eagle nest sites relative to control sites (~ 33% higher). Phosphorous is a limiting nutrient in coastal temperate forests, additions help to alleviate chlorosis and slow growth especially when paired with nitrogen. Higher potassium concentration also occurred on eagle-inhabited islands but was not associated specifically with current nest sites, perhaps reflecting differential persistence of macronutrients in the soil. Despite expectations, soil δ 15N abundance was not statistically higher at eagle nest sites. Total soil nitrogen was also not statistically higher at eagle nest sites. There were no significant differences between vegetation composition at eagle nest sites and reference sites, but reference sites tended to be dominated by shrub species. Additionally, I proposed that bald eagles (Haliaeetus leucocephalus) are vectors of nutrient subsidies, depositing nutrient-rich guano at nest sites, which could alter soil chemistry and vegetation composition. In an exploratory study of seven nest sites, I found higher soil phosphorous at eagle nest sites relative to control sites (~ 33% higher). Phosphorous is a limiting nutrient in coastal temperate forests, additions help to alleviate chlorosis and slow growth especially when paired with nitrogen. Higher potassium concentration also occurred on eagle-inhabited islands but was not associated specifically with current nest sites, perhaps reflecting differential persistence of macronutrients in the soil. I expected to observe elevated nitrogen isotope signatures (δ 15N) given bald eagles’ position in the trophic web and the potential for volatilization of guano but soil δ 15N abundance was not statistically higher at eagle nest sites. Total soil nitrogen was also not statistically higher at eagle nest sites. There were no significant differences between vegetation composition at eagle nest sites and reference sites, but reference sites tended to be dominated by shrub species / Graduate

Esta pesquisa se situa no campo do planejamento territorial e da sustentabilidade urbana, com foco na conservação da biodiversidade e na provisão de serviços ecossistêmicos. Parte-se do pressuposto que a urbanização, embora estimule o progresso econômico, social e cultural, tem impulsionado problemas como a degradação ambiental e a falta de saneamento e que é necessário um planejamento do território em nível metropolitano, visando a manutenção do capital natural e a provisão de serviços ecossistêmicos essenciais ao bem-estar humano e à qualidade de vida, de forma sustentável. Assim, o objetivo deste estudo foi propor um conjunto de indicadores de avaliação de capital natural e de serviços ecossistêmicos para regiões metropolitanas, tendo como recorte empírico a Região Metropolitana de Curitiba. O conjunto de indicadores foi construído a partir de uma base teórica e posteriormente validado por especialistas utilizando-se o método Delphi. O conjunto de indicadores proposto e validado por especialistas apresenta embasamento teórico e flexibilidade para aplicação em diferentes contextos. A situação na RMC demonstra uma tendência de degradação do capital natural e consequente comprometimento na provisão de água em longo prazo, com destaque para a bacia do rio Ribeira. Verificou-se a necessidade de se ampliar, em grande parte da RMC, a implementação das políticas de proteção da biodiversidade, estimular a criação de novas unidades de conservação e fortalecer o monitoramento do uso do solo. / This research is situated in the field of territorial planning and urban sustainability, with focus on biodiversity conservation and ecosystem service provision. The assumption is that urbanization, while stimulating economic, social and cultural progress, has driven problems such as environmental degradation and lack of sanitation and that it is necessary to plan the territory at the metropolitan level, aiming at maintaining natural capital and providing ecosystem services essential to human well-being and quality of life in a sustainable way. Thus, the objective of this study was to propose a set of indicators for the evaluation of natural capital and ecosystem services on metropolitan regions, having as an empirical cut the Metropolitan Region of Curitiba. The set of indicators was built from a theoretical basis and then validated by experts using the Delphi method. The set of indicators proposed and validated presents flexibility for application in different contexts. The situation in the MRC demonstrates a trend of degradation of natural capital and consequent impairment in long term water provision, with emphasis in the Ribeira river basin. It is necessary to expand implementation of policies to protect biodiversity, to stimulate the creation of new conservation units and to strengthen the monitoring of land use.

Dans les domaines de la modélisation et de la simulation numérique, les simulateurs développés prennent parfois en compte de nombreux paramètres dont l'impact sur les sorties n'est pas toujours bien connu. L'objectif principal de l'analyse de sensibilité est d'aider à mieux comprendre comment les sorties d'un modèle sont sensibles aux variations de ces paramètres. L'approche la mieux adaptée pour appréhender ce problème dans le cas de modèles potentiellement complexes et fortement non linéaires repose sur la décomposition ANOVA et les indices de Sobol'. En particulier, ces derniers permettent de quantifier l'influence de chacun des paramètres sur la réponse du modèle. Dans cette thèse, nous nous intéressons au problème de l'estimation des indices de Sobol'. Dans une première partie, nous réintroduisons de manière rigoureuse des méthodes existantes au regard de l'analyse harmonique discrète sur des groupes cycliques et des tableaux orthogonaux randomisés. Cela nous permet d'étudier les propriétés théoriques de ces méthodes et de les généraliser. Dans un second temps, nous considérons la méthode de Monte Carlo spécifique à l'estimation des indices de Sobol' et nous introduisons une nouvelle approche permettant de l'améliorer. Cette amélioration est construite autour des hypercubes latins et permet de réduire le nombre de simulations nécessaires pour estimer les indices de Sobol' par cette méthode. En parallèle, nous mettons en pratique ces différentes méthodes sur un modèle d'écosystème marin. / In the fields of modelization and numerical simulation, simulators generally depend on several input parameters whose impact on the model outputs are not always well known. The main goal of sensitivity analysis is to better understand how the model outputs are sensisitive to the parameters variations. One of the most competitive method to handle this problem when complex and potentially highly non linear models are considered is based on the ANOVA decomposition and the Sobol' indices. More specifically the latter allow to quantify the impact of each parameters on the model response. In this thesis, we are interested in the issue of the estimation of the Sobol' indices. In the first part, we revisit in a rigorous way existing methods in light of discrete harmonic analysis on cyclic groups and randomized orthogonal arrays. It allows to study theoretical properties of this method and to intriduce generalizations. In a second part, we study the Monte Carlo method for the Sobol' indices and we introduce a new approach to reduce the number of simulations of this method. In parallel with this theoretical work, we apply these methods on a marine ecosystem model.

The solar radiation in general and UV radiation in particular has been recognized to stimulate plant litter decomposition through photochemical mineralization of photosensitive organic molecules, such as lignin, facilitating microbial decomposition, with great relevance role in dryland ecosystems where microbial activity is low, however little is known about how photodegradation could influence other ecosystems without moisture limitations and under what conditions may be favored, therefore the mechanisms has not yet been established. Decomposition in tropical ecosystem is a complex process and can be induced by a number of environmental factors with certain differences when compared to arid and semi-arid ecosystems. To assess the mechanisms underlying UV-B photodegradation, we designed a 300 days field experiment at a tropical ecosystem with high levels of annual precipitation and exposure litter to three levels of radiation combined with a biocide treatment. Results show that the removal of UV-B radiation decelerated plant litter decomposition during the later stage compared to litter exposure to full sun, however shaded litter had similar mass loss compared to litter exposed to full sun. Furthermore, differences in the decay constant among radiation treatments due to UV-B effect is independent of lignin loss. Overall, our study suggest that UV-B contributes to the plant litter decomposition through carbon losses, however, had no effect on nitrogen, neither lignin nor cellulose loss. However, more studies are needed in order to investigate the positive and negative effects of UV exposure on microbial activity in tropical ecosystems. / A radiação solar em geral e a radiação ultravioleta (UV) em particular têm sido reconhecida por estimular a decomposição da serapilheira através da mineralização fotoquímica de moléculas fotossensíveis, como a lignina, facilitando a decomposição microbiana, com um papel de grande relevância em ecossistemas áridos onde a atividade microbiana é baixa, no entanto pouco se sabe como a fotodegradação pode influenciar outros ecossistemas como por exemplo os mais úmidos e sob quais condições a fotodegradação é favorecida, portanto os mecanismos ainda não foram estabelecidos. Decomposição em ecossistemas tropicais é um processo complexo e pode ser influenciado por vários fatores ambientais e com certas diferenças quando comparada com ecossistemas áridos e semiáridos. Para avaliar os mecanismos subjacentes à fotodegradação via radiação UV-B, um experimento de campo de 300 dias foi estabelecido em um ecossistema tropical com alto índice de precipitação anual onde a serapilheira foi exposta a três níveis de radiações diferentes, combinada com um tratamento com biocida. Resultados mostram que a remoção da radiação UV-B desacelerou a decomposição da serapilheira durante o último estágio do experimento comparado com a serapilheira exposta a radiação ambiente, no entanto a serapilheira quando sombreada teve perda de massa similar à exposta a radiação ambiente. Além disso, diferenças na taxa de decaimento entre os tratamentos de radiação devido ao efeito da radiação UV-B foram independentes da perda de lignina. No geral, nosso estudo sugere que a radiação UV-B contribui com a decomposição da serapilheira através da perda de carbono, no entanto não teve efeito na perda de massa de nitrogênio, lignina e celulose. Portanto, mais estudos são necessários para investigar o efeito positivo e negativo da exposição à radiação UV-B na atividade microbiana e na decomposição da serapilheira em ecossistemas tropicais.

L’aquaculture des ormeaux (Haliotis spp.) fournit un bon cas étude des défis techniques, sociaux et environnementaux concernant l'introduction d’organismes marins nouvellement domestiqués sur le marché. En effet, l'élevage de l'ormeau a débuté au Japon au cours des années 1960 et, d'après la FAO (2012), est aujourd'hui l'une des industries aquacoles les plus dynamiques dans le monde. Pourtant, toutes les tentatives n'ont pas connues le même succès. Cette dissertation vise à comprendre les facteurs qui ont permis ou entravé le succès environnemental, social et technique de l’halioticulture, en mettant l'accent sur la France. Plus précisément, nous examinons trois grandes catégories de facteurs qui peuvent expliquer son évolution: - L'importance des facteurs socio-écologiques dans la gestion de la ressource en ormeaux - Les facteurs socioculturels qui étayent le statut de l'ormeau en tant que ressource éligible pour l'aquaculture. - Le rôle de la science dans l'industrialisation de l'élevage d'ormeaux. / Abalone aquaculture (Haliotis spp.) or halioticulture provides a good case study of the technical, social and environmental challenges of introducing newly domesticated marine organisms into the market. Abalone farming began in Japan in the 1960s and, according to FAO (2012), is now one of the most dynamic aquaculture industries in the world. Yet all attempts have not met with the same success. This essay aims to understand the factors that have allowed or hindered the environmental, social and technical success of halioticulture, with an emphasis on France.More specifically, we examine three main categories of factors that may explain its evolution:- The importance of socio-ecological factors in the management of abalone resource.- Socio-cultural factors that support the status of the abalone as an eligible resource for aquaculture.- The role of science in the industrialization of abalone farming.

Os ecossistemas costeiros do litoral do Estado de São Paulo apresentam-se ambientalmente degradados, em conseqüência da concentração populacional e instalação de pólos industriais e petroquímicos. O lançamento de rejeitos urbanos, industriais e portuários nos corpos d’água resulta no aporte indiscriminado de poluentes nos ambientes estuarinos. O metal pesado mercúrio (Hg) constitui um dos poluentes de maior risco para o equilíbrio ecológico e saúde humana, justificado pela elevada toxicidade e potencial de bioacumulação e biomagnificação através da cadeia alimentar. O presente trabalho teve por objetivos: (a) determinar o conteúdo de Hg total em solos de manguezais da Baixada Santista (rio Cascalho, Mariana, Crumahú, Irirí e Canal da Cosipa) e Ilha do Cardoso; (b) verificar a influência do preparo de amostras na determinação de Hg em solos estuarinos; (c) analisar parâmetros químicos relacionados à geoquímica do Hg; (d) avaliar a distribuição do conteúdo total de Hg nas frações mais relevantes em solos de manguezais por meio de extração seqüencial. Foi determinado um enriquecimento de Hg nas camadas superficiais dos solos da Baixada Santista e Ilha do Cardoso, porém não foi verificado um padrão uniforme de distribuição das concentrações de Hg total nos perfis amostrados. O manguezal do rio Cascalho e Canal da COSIPA apresentaram elevados teores de Hg (5,65 mg.kg-1 e 1,64 mg.kg-1, respectivamente), caracterizando áreas altamente poluídas. Em uma nova amostragem nos manguezais do rio Cubatão, próximo ao rio Cascalho, os teores de Hg total permaneceram em concentrações superiores ao valor de alerta estabelecido pela CETESB. Em função da variabilidade dos resultados, a influência do preparo de amostras desta natureza foi analisada comparando-se teores totais em amostras úmidas, sem tratamentos prévios de secagem e moagem; secas pelo processo de liofilização, sem posterior moagem; amostras submetidas aos pré-tratamentos de secagem em estufa a 25°C e moagem; amostras submetidas aos pré-tratamentos de secagem em estufa a 180°C e moagem. Uma vez que a umidade em cada sub-amostra pode diferir, a determinação do conteúdo de Hg total na amostra úmida pode ser a causa primária da variabilidade entre os resultados, somada à distribuição desuniforme de raízes e matéria orgânica, e variações texturais. A utilização de amostras secas pelo processo de liofilização e posteriormente moídas pode contribuir para a precisão analítica dos resultados gerados da extração de Hg, em solos de ambientes estuarinos. Com base nos resultados de Eh e pH foi verificado que a quase totalidade das amostras se situam no campo de estabilidade do Hg elementar e em menor proporção no campo de estabilidade do HgS, favorecendo a formação de Hg elementar e o processo de piritização. De acordo com a extração seqüencial, o Hg encontra-se associado principalmente à fração pirítica, enquanto que nas frações mais reativas dos solos, foram determinadas menores concentrações de Hg (frações trocável e solúvel, carbonatos, ferridrita e lepidocrocita), e concentrações ainda relativamente inferiores na fração correspondente às formas cristalinas de ferro, em todas as amostras. / Coastal ecosystems at São Paulo State show a condition of environmental degradation, due to high density of population and presence of industrial and petrochemical poles. The disposal of urban, industrial and harbor wastes into water bodies result in indiscriminate accumulation of pollutants in estuarines soils. Mercury (Hg) constitutes one of the most dangerous pollutants for the ecological balance and human health, because of its high toxicity and potential for bioaccumulation and biomagnification through the food chain. The current study aimed to: (a) determine total content of Hg in mangroves soils from Baixada Santista region (Cascalho, Mariana, Crumahú and Irirí rivers and COSIPA Channel) and Cardoso Island; (b) verify the effects of sample pre-treatments to determine Hg in mangrove soils; (c) analyze chemical parameters related to the geochemistry of Hg; (d) evaluate the distribution of total Hg in the most relevant fractions of mangroves soils using sequential extraction technique. An enrichment in total Hg was observed in the superficial layers of Baixada Santista and Cardoso Island soils, although there was no regular pattern of total Hg distribution at the studied profiles. Mangroves soils from Cascalho River and COSIPA Channel showed high Hg concentrations (5,65 mg.kg-1 e 1,64 mg.kg-1, respectively), characterizing highly polluted areas. Soil samples from Cubatão River, close to Cascalho River, showed total Hg values greater than the alert level established by CETESB. Due to the great data variability the effects of sample pre-treatment were analyzed by comparing the total Hg contents in fresh samples without previous drying and grinding treatments with samples dried by liofilization without later grinding; samples submitted to pre-drying treatments at 25°C and grinding, samples submitted to pre-drying treatments at 180°C and grinding. Since water content may vary from one sample to another, the establishment of total Hg content in fresh samples can be the main cause of results variability, in addition to the non-uniform distribution of root, organic matter and textural variations. The use of liofilized and ground samples may contribute to the analytical precision. Regarding Hg geochemistry, data obtained from Eh-pH correlation evidence that most samples are located in the elemental Hg stability field and, in a smaller proportion, in the HgS stability field, favoring elemental Hg formation and pyritization process. Sequential extraction data show that Hg is mainly associated to the pyritic fraction, followed by the most reactive fractions (soluble, exchangeable, carbonates, ferrihydrite and lepdocrocite fractions), and the fraction corresponding to crystalline iron oxides.

A perspectiva de elaborar planejamentos em áreas de floresta a partir da concepção de serviços ecossistêmicos, principalmente serviços hídricos, expandiu-se em muito pouco tempo, principalmente porque essa estratégia evidencia, claramente, como a perda desse sistema natural por atividades do homem pode causar perdas irreparáveis ao seu próprio bem-estar. Trabalhar planejamento ambiental sob esse enfoque exige adoção de uma forte base teórica sobre serviços hídricos; um modelo que permita comparar paisagens em diferentes condições de uso e conservação de floresta; e clareza sobre o tipo de valoração empregado na identificação dos limites de perdas de floresta, de forma a não comprometer a oferta necessária desses serviços. Este estudo defende que em Mata Atlântica é possível reconhecer a dependência direta entre quantidades de floresta, de usos humanos e de serviços hídricos em bacias hidrográficas e, por ela, valorar a oferta desses serviços e comparar paisagens. Para fortalecer esse argumento foram selecionadas 27 unidades territoriais da região do Vale do Paraíba (São Paulo, Br) a partir da aplicação de um modelo espacial baseado na extração de bacias hidrográficas originadas da intersecção de hexágonos com percentuais de floresta, campo antrópico e silvicultura. Em cada unidade foram obtidos dados de 13 parâmetros físico-quimico-biológicos nas estações seca e chuvosa, que foram hierarquizados pelo Random Forest de forma a aferir suas relações com a quantidade de floresta e formular índices de sete serviços hídricos. Os parâmetros que se mostraram mais adequados para monitoramento dos serviços hídricos frente aos ganhos de floresta, com grande peso hierárquico na formulação dos índices, foram turbidez, condutividade e sólidos totais dissolvidos. Os índices obtidos permitiram apontar que para manter 50% do potencial total dos sete serviços hídricos em paisagens com campos antrópicos são necessárias mais de 40% de cobertura florestal, mas os melhores ganhos potenciais de serviços ocorreram a partir de 60%. Quando a unidade territorial é a combinação entre floresta e silvicultura há maior oferta de serviços hídricos com menor quantidade de floresta, porém o manejo da madeira e a presença de áreas degradadas mudaram completamente esse cenário favorável / The possibility of elaborate planning in forest areas from notion of ecosystem services, especially water services, expanded in a short time, mainly because this strategy shows how the loss of this natural system by human activities can cause losses irreparable to their own well-being. Working environmental planning under this approach requires the adoption of a strong theoretical basis for water services; a model for comparing landscapes under different conditions of use and conservation of forest; and insight about the type of valuation used in the identification of the limits of forest loss in order not to compromise the necessary supply of these services. This study argued that in the Atlantic Forest is possible to recognize the direct dependency between amounts of forest, human uses and water services in watersheds and, through it, assess the provision of these services and compare landscapes. In order to defend this argument were selected 27 territorial units of the Vale do Paraíba region (Sao Paulo, Brazil) from the application of a spatial model based on the extraction of river basins originating hexagons intersection with percentages of forest, anthropic field and forestry. In each unit were obtained data from 13 physical-chemical-biological parameters in two seasons, which were ranked by Random Forest in order to support its relations with the amount of forest as well as the index formulation for seven water services. The parameters more suitable for monitoring water in front of services to forest gains, and with large hierarchical influence the formulation of the indices, were turbidity, conductivity and total dissolved solids. The indices obtained allowed to point out that to keep 50% of the total potential of the seven water services in landscapes with anthropic fields (pasture) are required more than 40% forest cover, but the best servi4e potential gains occurred from 60%. When the territorial unit is a combination of forest and forestry there is more supply of water services with less forest, but the management of timber and the presence of degraded areas completely changed this favorable scenario

Este trabalho teve por objetivo avançar no entendimento acerca do constructo \"ecossistema\" quando aplicado na área da teoria organizacional, especificamente dentro do campo da gestão da inovação. A investigação desse termo foi considerada importante por despertar o interesse tanto da comunidade científica quanto dos empreendedores. Após uma revisão de literatura com as principais publicações sobre esse tema, percebeu-se que essa abordagem utilizava conceitos de outras teorias, principalmente da teoria de sistemas, cadeia de valor e teoria de rede. A partir dessa constatação, o estudo chegou a seguinte questão de pesquisa: \"Como a abordagem de Ecossistema explica - de maneira original em relação à cadeia de valor e a teoria de rede - a atividade empreendedora de base tecnológica em sua fase nascente?\". Dessa discussão, intuiu-se que a lógica de ecossistema propiciava maiores benefícios em momentos de instabilidade de um empreendimento, onde o caminho a ser perseguido era incerto. Essa característica, se confirmada seria de particular proveito para os empreendimentos inseridos nos chamados mercados dinâmicos, que são ambientes constantemente envoltos em incertezas. A fim de testar essa hipótese, foram formuladas quatro proposições: (P1) A lógica de ecossistema tem boa aderência em empreendimentos nascentes, pois ela consegue adaptar-se às mudanças que não foram previstas no escopo inicial do planejamento; (P2) A cadeia de valor, pelo fato de não considerar atores complementadores perde capacidade de avaliar um empreendimento em fase inicial; (P3) A cadeia de valor, por se tratar de uma ferramenta de análise de melhoria contínua, tem dificuldades para lidar com mudanças disruptivas, que altere seu estado estável; (P4) O mapeamento de uma rede pode ser difícil e custoso, dificultando sua execução prática. A investigação dessas proposições foi conduzida por meio de entrevistas semiestruturadas realizadas com quatro empresas nascentes de base tecnológica. Em relação à proposição (P1), concluímos que além da adaptação esperada da abordagem ecossistema perante as mudanças ocorridas na trajetória planejada do empreendimento, o ecossistema consegue ainda sugerir que metas concretas a serem perseguidas pelo empreendimento a fim de conseguir sobreviver e evoluir para os próximos estágios. A proposição (P2) também foi confirmada na pesquisa de campo, sugerindo que os atores complementadores são sim de relevância significante para que o cliente reconheça valor à oferta da firma focal. Além disso, esta pesquisa sugere ainda que de todos os complementos da sua oferta, devem ser monitorados com especial cuidado aqueles que estão na fronteira da inovação tecnológica. A proposição (P3) não pode ser verificada neste trabalho e a proposição (P4) precisa de maior verificação para uma conclusão segura. A pesquisa conclui com um posicionamento otimista em relação à abordagem \"ecossistema\", acreditando ser uma abordagem promissora para o gerenciamento de empreendimentos inseridos em ambientes de alta velocidade. Por se tratar de um estudo exploratório, sugere que as investigações não se encerrem neste trabalho, apresentando por fim alguns caminhos que podem ser aprofundados. / This dissertation is an attempt to push forward the knowledge boundary concerning the construct \"ecosystem\" placed in the organizational theory, precisely within the innovation management field. The investigation was considered important because it arouses the interest both of the scientific community than the managers and entrepreneurs. After the literature review, which included the most important publications about the theme, it came to our sight that this approach use concepts of different theories, mainly the system theory, the value chain and the network theory. Along with this finding, this study arrive to the following research question: \"How does the Ecosystem approach explains - in an original basis facing the value chain and the network theory - the entrepreneurship activity in technological startups?\". From this debate, an insight sparkles, saying that the ecosystem logic offers more benefits during the instability phases of a business, when the way to follow is uncertain. This feature, assuming to be right, would be particular useful for organizations placed in dynamic markets, which are environments surrounded by uncertainties. In order to test this hypothesis, four propositions were formulated: (P1) The ecosystem logic has good adherence in startups, because it can adapt to the changes that were not expected in the first planning scope; (P2) because the value chain do not consider the complementors, it loses capability to evaluate a business startup; (P3) the value chain is a tool for continuous improvement , and therefore, have difficulties to deal with disruptive changes that modifies an organization stable state; (P4) mapping a network is complex and costly, hampering its practical execution. The investigation of these propositions was conducted through semi structured interviews performed with four startups. Concerning to the proposition (P1), we concluded that beyond the adaptation expected in the first place, the ecosystem can also suggest objective goals to be chased so that the ecosystem can survive and evolve to the next stage. The proposition (P2) was also confirmed in the field research, suggesting that the complementors have significant relevance on the client\'s value perception regarding the focal firms\' offer. Besides, this research also proposes that not every complementors should be tracked from the focal firm, but only the ones that are at the technological innovation boundary edge. The proposition (P3) couldn\'t be verified in this study while the proposition (P4) needs more investigation to a reliable conclusion. The research concludes with a positive perspective about the ecosystem, believing it is a promising approach for organizations residing in high velocity markets. Since this is an exploratory study we strongly suggest that the investigations do not end in this dissertation, where is presented some possible paths so that this work can be continued.

Os manguezais compõem um bioma de transição entre o ambiente marinho e terrestre característico de regiões tropicais e subtropicais. Dentro deste ambiente, a diversidade microbiana desempenha um importante papel na ciclagem de nutrientes. No entanto, a diversidade de fungos em manguezais é pouco estudada, bem como o estabelecimento de relações ecológicas entre esses organismos e o ambiente. Neste intuito, o presente trabalho teve como objetivo comparar a diversidade fúngica no sedimento de dois manguezais localizados na cidade de Bertioga [um contaminado com petróleo (OilMgv), e um manguezal próximo (AntMgv)]. As análises se basearam em metodologias independentes de cultivo, como a técnica de PCR-DGGE baseada na região ITS (internal transcribed sequence), que mostrou padrões complexos e uma grande abundância de bandas, sendo que dentro dos perfis obtidos é possível observar grupos selecionados para cada manguezal e para cada região dentro de um mesmo manguezal. A construção e análise de bibliotecas de clones também desta região mostraram a dominância de fungos afiliados aos filos Basidiomycota e Ascomycota, com destaque para a ocorrência dos gêneros Epicoccum, Nigrospora e Cladosporium. Uma análise mais ampla, por meio de pirosequenciamento permitiu uma melhor visualização destas comunidades e corroborou os dados anteriores, comprovando a ocorrência de grupos fúngicos distintos nos manguezais estudados (sequenciamento baseado em amplicons da região ITS), e permitindo a observação de outros grupos eucarióticos nos manguezais avaliados (análise de metagenômica). Em resumo, os dados apresentados constituem a primeira descrição robusta destas comunidades que desempenham funções essenciais à manutenção e ao funcionamento do ambiente estudado. / Mangroves ecosystems make up a transition biome between land and marine environments, particular occurring in tropical and subtropical regions. Within this environment, the microbial diversity plays an important role in nutrient cycling. However, the diversity of fungi in mangrove is still poorly studied, as well as the establishment of ecological relationships between these organisms and the environment. In this sense, this study aimed to compare the fungal diversity in the sediment of two mangroves located in the city of Bertioga [an oil-spilled mangrove (OilMgv) and a nearby unaffected mangrove (AntMgv)]. Analyzes were based on culture-independent methods, such as PCR-DGGE based on the ITS region (internal transcribed sequence), which showed complex patterns and an abundance of bands, allowing the observations of groups selected for each mangrove and each region inside the same mangrove. The construction and analysis of clone libraries showed the dominance of fungi affiliated with the phyla Ascomycota and Basidiomycota, with a remark for the occurrence of the genera Epicoccum, Nigrospora e Cladosporium. A more robust approach, performed by pyrosequencing, allowed better visualization of these communities, corroborating the previous results, reinforcing the differential occurrence of fungi groups in assessed mangroves (based on sequencing of the ITS region amplicons), and allowing the observation of other eukaryotic groups in these areas (metagenômica analysis). In summary, the present data provide the first robust description of these communities that perform essential roles for the functioning and maintenance of the studied environment.

Les biotechnologies mobilisées aujourd'hui dans les bioindustries requièrent des compétences qu'une entreprise ne peut plus maîtriser seule. L'aboutissement d'innovations disruptives implique une approche pluridisciplinaire nécessitant l'intervention de plusieurs secteurs industriels ce qui s'oppose à une logique d'innovation propriétaire. La transversalité nécessaire à ces rapprochements entre entreprises privées et secteurs public et ou entre industries issues de secteurs d'activités différents modifie profondément la nature des modèles d'organisation. Parmi l'ensemble des modèles adoptés, celui des écosystèmes d'affaires (EA) occupe une place de plus en plus centrale dans les bioindustries. L'optimisation des logiques d'innovation associées est devenue un enjeu auquel même les institutions tentent de répondre en soutenant l'adoption de logiques d'innovation ouverte (IO) et le développement d'EA. Pourtant la nature de ces deux notions restent discutées et leur combinaison théorique mal comprise. Bien que les études de cas montrent que les EA appuient leur développement sur l'IO dans les domaines dont le développement repose sur la connaissance, la nature et la séquence de mise en œuvre des concepts mobilisés restent à déterminer. De plus, le débat demeure concernant la nature ontologique et les limites épistémologiques des notions d'IO et d'EA. Cette thèse s'efforce de préciser ces éléments puis elle détermine quel rôle est joué par l'IO dans l'émergence des EA dans les bioindustries françaises.L'analyse ontologique de la notion d'EA révèle la valeur purement métaphorique des transpositions effectuées par Moore depuis l'écologie pour en établir la définition. Par conséquent, l'approche analogique adoptée par une partie des scientifiques ne peut être retenue pour établir les limites épistémologiques de la notion d'EA. La nature ontologique de la notion d'IO reste incertaine. Nature des flux d'informations inter-organisationnels et capacités dynamiques des firmes sont conjointement mobilisés. Cette incertitude n'étant pas permissive à la réalisation d'une analyse épistémologique, les capacités dynamiques sont ici choisies comme fondement théorique de l'IO. L'analyse épistémologique de la notion d'EA démontre l'application d'une boucle récursive dans sa construction. De plus, elle révèle l'existence d'une théorie substantive derrière la notion d'EA, théorie mobilisant une séquence de concepts mise en œuvre successivement dans l'émergence des EA. L'IO étant l'une des notions mobilisées.La posture épistémologique adoptée dans cette thèse est celle du réalisme critique. Elle permet la prise en compte de la boucle récursive, est adaptée à l'approche par les théories ancrées, et intègre les circonstances intrinsèques et extrinsèques justifiant la manière dont les mécanismes générateurs sont activés. Elle autorise la formulation d'hypothèses fondatrices d'ordre ontologique. Ce choix permet de conserver la posture épistémologique séminale implicite de Moore, de légitimer la valeur de sa démarche ancrée, d'assumer l'hypothèse ontologique formulée à propos des fondements de l'IO, et de tenir compte des facteurs tant environnementaux qu'organisationnels justifiant de l'émergence des EA. La méthodologie d'analyse qui en découle est qualitative. Elle passe par une comparaison de deux études de cas réalisées sur la base d'analyses de données secondaires. Les facteurs contextuels de chaque cas sont corrélés afin de dévoiler les mécanismes générateurs justifiant du rôle de l'IO dans l'émergence des EA.Les résultats confirment la séquence de mise en œuvre des concepts proposés par Moore dans l'émergence des EA pour les bioindustries françaises. Ils précisent la place de l'IO dans cette séquence en spécifiant son rôle dans le passage de la collaboration à la coévolution des firmes au sein de l'EA. Ils confirment que l'EA ne constitue pas un modèle d'organisation en soi mais une posture inter-firmes favorisant l'adoption de modèles adaptés. / Biotechnologies mobilized today in bio-industries require skills that companies can no longer control alone. The development of disruptive innovations involves a multidisciplinary approach requiring the intervention of several industrial sectors that is opposed to proprietary innovation logic. Transversality necessary for these collaborations between private companies and public sectors and or between industries from different business sectors profoundly changes the nature of organizational models chosen by firms. Among all the models adopted, the business ecosystem (BE) occupies a more and more central place in bio-industries. The optimization of the associated logical innovation has become a challenge that even the institutions are trying to respond by supporting the adoption of open innovation logics (OI) and the development of BE. Yet, the nature of these two notions is still discussed and there theoretical combination remains poorly understood. Although case studies show that BE support their development on OI in knowledge based industries, nature and implementation sequence of underlying concepts remain to be determined. Moreover, the debate remains regarding the ontological and epistemological limits of OI and BE notions. This thesis seeks to clarify these elements and determines what role is played by the OI in the emergence of BE in the French bio-industries.The ontological analysis of BE notion reveals the purely metaphorical transpositions made by Moore from ecology to establish its definition. Therefore, the analogical approach supported by a part of the scientific community can't be applied to establish the epistemological limits of BE notion. The ontological nature of OI notion remains uncertain. Nature of inter-organizational information flows and dynamic capabilities of firms are jointly mobilized. This uncertainty is not permissive to the achievement of an epistemological analysis, therefore dynamic capacities here were chosen as theoretical foundations of the OI notion. The epistemological analysis of the development of BE notion demonstrates the application of a recursive loop in its construction. Moreover, it reveals the existence of a substantive theory behind the BE notion, theory which mobilizes a sequence of concepts implemented successively in the emergence of BE. The OI is one of mobilized notions.The epistemological posture adopted in this thesis is that of critical realism. It allows the inclusion of the recursive loop. It is suitable for the approach by grounded theories. It integrates intrinsic and extrinsic circumstances justifying how generating mechanisms are activated. It allows the formulation of founding ontological assumptions. This choice preserves Moore's implicit epistemological posture, legitimizes the value of its grounded approach, assumes the ontological assumption made about the foundations of OI notion, and takes into account both environmental and organizational factors justifying the emergence of BE. The resulting methodology is qualitative. It goes through a comparison of two case studies based on secondary data analysis. Contextual factors of each case are correlated to reveal the generative mechanisms justifying the role of OI in the emergence of BE.The results confirm the implementation sequence of concepts proposed by Moore in the emergence of BE in the French bio-industries. They precise the place of OI in this sequence by specifying its role in the transition from collaboration to co-evolution of firms within BE. They confirm that BE is not an organizational model in itself, but an inter-organizational stance promoting the adoption of appropriate models.