Polyurethane and the analogous ‘polyurethane-urea’ are high performance polymeric materials having remarkable properties such as high stiffness, abrasion and tear strengths. In many studies, the low strength rubbers have been blended with various types of polyurethanes for new and improved materials. However, until now, the reported heterogeneous blends offer only a narrow temperature range of application due to the high temperature softening of their polyurethane (-urea) phase. In addition, the conventional solution-or melt-blending methods are time and energy intensive, which tend to forfeit the economical realization of the reported blends. In contrast to earlier studies, a simplified reactive blending process is suggested to synthesize polyurethane-urea via a prepolymer route during blending with rubbers to obtain novel elastomeric materials having extended performance characteristics. The reactive blending process is opted to prepare blends based on nitrile butadiene rubber (NBR) and in-situ synthesized polyurethane-urea (PUU). The blending is carried out in an internal mixer at a preset temperature of 100°C. The critical temperatures of the reactive blending process are determined from the chemo-rheological analysis of a premix, composed of a 4,4′-diphenylmethane diisocyanate (MDI)/polyether (PTMEG) based prepolymer admixed with 1,3-phenylene diamine (mPD). The prepared NBR/PUU blends exhibit highly improved mechanical properties. Contrary to previous reports, the reinforced dynamic-mechanical responses of the novel blends remain stable till very high temperatures (≥180°C). The influence of diamine type on the in-situ synthesized polyurethane-urea and the performance of prepared blends are investigated. Four different diamines, namely 1,3-Phenylene diamine, 1,4-Bis(aminomethyl)benzene, 4,4′-Methylene-bis(2-chloroaniline) and 4,4ʹ-(1,3-Phenylenediisopropylidene)bisaniline, are selected to chain extend the prepolymer to PUU during blending with NBR. The chemical and domain structure of the PUUs are found to greatly influence the reinforced tensile and dynamic-mechanical responses of the NBR/PUU 70/30 blends. The PUU (based on MDI/PTMEG prepolymer and mPD) is blended with polar (CR, XNBR) and nonpolar (NR, EPDM, sSBR) rubbers. PUU compatibilizes with all the rubbers irrespective of their polarity and reinforces their tensile and dynamic-mechanical characteristics. The use of blends in industrial applications, for example, in a truck tire tread compound and as a roller covering material, is examined. In a simplified tire tread formulation, the carbon black for NR-CB composite is partially replaced with an equivalent quantity of PUU for NR/PUU-CB composite of similar hardness. The dynamic mechanical investigations reveal that the energy dissipation and strain dependent softening is high in NR-CB as compared to the NR/PUU-CB composite. In another application, NBR/PUU blend is successfully tested as a rubber roller covering material. The tested blend-covered roller retains its structural integrity and develops less heat build-up as compared to the silica filled NBR-covered roller. This shows a substantial suitability of the blend-covered rollers for film, printing and textile processing machinery. These novel blends are considered to be the promising new materials for many commercial applications including wheels, rubber rollers, belts or pump impellers.

Polyurethane and the analogous ‘polyurethane-urea’ are high performance polymeric materials having remarkable properties such as high stiffness, abrasion and tear strengths. In many studies, the low strength rubbers have been blended with various types of polyurethanes for new and improved materials. However, until now, the reported heterogeneous blends offer only a narrow temperature range of application due to the high temperature softening of their polyurethane (-urea) phase. In addition, the conventional solution-or melt-blending methods are time and energy intensive, which tend to forfeit the economical realization of the reported blends. In contrast to earlier studies, a simplified reactive blending process is suggested to synthesize polyurethane-urea via a prepolymer route during blending with rubbers to obtain novel elastomeric materials having extended performance characteristics. The reactive blending process is opted to prepare blends based on nitrile butadiene rubber (NBR) and in-situ synthesized polyurethane-urea (PUU). The blending is carried out in an internal mixer at a preset temperature of 100°C. The critical temperatures of the reactive blending process are determined from the chemo-rheological analysis of a premix, composed of a 4,4′-diphenylmethane diisocyanate (MDI)/polyether (PTMEG) based prepolymer admixed with 1,3-phenylene diamine (mPD). The prepared NBR/PUU blends exhibit highly improved mechanical properties. Contrary to previous reports, the reinforced dynamic-mechanical responses of the novel blends remain stable till very high temperatures (≥180°C). The influence of diamine type on the in-situ synthesized polyurethane-urea and the performance of prepared blends are investigated. Four different diamines, namely 1,3-Phenylene diamine, 1,4-Bis(aminomethyl)benzene, 4,4′-Methylene-bis(2-chloroaniline) and 4,4ʹ-(1,3-Phenylenediisopropylidene)bisaniline, are selected to chain extend the prepolymer to PUU during blending with NBR. The chemical and domain structure of the PUUs are found to greatly influence the reinforced tensile and dynamic-mechanical responses of the NBR/PUU 70/30 blends. The PUU (based on MDI/PTMEG prepolymer and mPD) is blended with polar (CR, XNBR) and nonpolar (NR, EPDM, sSBR) rubbers. PUU compatibilizes with all the rubbers irrespective of their polarity and reinforces their tensile and dynamic-mechanical characteristics. The use of blends in industrial applications, for example, in a truck tire tread compound and as a roller covering material, is examined. In a simplified tire tread formulation, the carbon black for NR-CB composite is partially replaced with an equivalent quantity of PUU for NR/PUU-CB composite of similar hardness. The dynamic mechanical investigations reveal that the energy dissipation and strain dependent softening is high in NR-CB as compared to the NR/PUU-CB composite. In another application, NBR/PUU blend is successfully tested as a rubber roller covering material. The tested blend-covered roller retains its structural integrity and develops less heat build-up as compared to the silica filled NBR-covered roller. This shows a substantial suitability of the blend-covered rollers for film, printing and textile processing machinery. These novel blends are considered to be the promising new materials for many commercial applications including wheels, rubber rollers, belts or pump impellers.

No description available.

Metastatic breast cancer treatment has seen few advances in recent years, yet treatment resistance continues to rise, causing disease recurrence. A pilot study was performed to determine the efficacy of ex vivo expansion and reprogramming of tumor-reactive immune cells from experimental metastatic tumor-sensitized mice. Also, phenotypic changes in tumors due to metastasis or tumor microenvironment influences were characterized. Metastatic neu+ mouse mammary carcinoma (mMMC) and its distant relapsing neu-antigen-negative variant (mANV) were investigated in FVBN202 mice. Tumor-reactive central memory CD8+ T cells and activated NK/NKT cells were successfully reprogrammed and expanded during 6-day expansion from mMMC- and/or mANV-sensitized mice, resulting in tumor-specific cytotoxicity. mMMC exhibited a flexible neu-expression pattern and acquired stem-like, tumorigenic phenotype following metastasis while mANV remained stable except decreased tumorigenicity. Myeloid-derived suppressor cell (MDSC) levels were not increased. Adoptive cellular therapy (ACT) with reprogrammed tumor-reactive immune cells may prove effective prophylaxis against metastatic or recurrent breast cancer.

In dieser Arbeit werden die Folgen der Calvo-Annahme in dynamischen makroökonomischen Modellen untersucht. Dafür wird die Calvo-Annahme unter Anwendung des Konzepts der statistischen Hazardfunktion verallgemeinert. Ich untersuche zwei mögliche Anwendungen dieses Ansatzes innerhalb von DSGE-Modellen. Im ersten Artikel zeige ich, dass der Zugewinn an Handhabbarkeit, der aus der Calvo-Annahme für Neu-Keynesianische Modelle folgt, mit unerwünschten Folgen in Bezug auf die Inflationsdynamiken einher geht. Der zweite Artikel schätzt die aggregierte Hazardfunktion unter Verwendung des theoretischen Rahmens des ersten Artikels. Es zeigt sich, dass die Annahme einer konstanten Hazardfunktion, die aus der Calvo-Annahme folgt, von den Daten eindeutig abgelehnt wird. Im dritten Artikel analysiere ich die Implikationen der empirisch geschätzten Hazardfunktion für die Persistenz von Inflation und die Geldpolitik. Die Untersuchungen zeigen, dass mittels der empirisch plausiblen aggregierten Hazardfunktion Zeitreihen simuliert werden können, die mit der Persistenz der inflatorischen Lücke im US Verbraucherpreisindex konsistent sind. Anhand dieser Ergebnisse komme ich zu dem Schluss, dass die Hazardfunktion eine entscheidende Rolle für die dynamischen Eigenschaften von Inflation spielt. Der letzte Artikel wendet den selben Modellierungsansatz auf ein Real-Business-Cycle Model mit rigidem Arbeitsmarkt an. Unter Verwendung eines allgemeineren stochastischen Anpassungsprozess stelle ich fest, dass die Arbeitsmarktdynamiken von einem Parameter beinflusst werden, der das Monotonieverhalten der Hazardfunktion bestimmt. Insbesondere steigt die Volatilität des Beschäftigungsniveaus, wohingegen dessen Persistenz mit zunehmendem Parameterwert abnimmt. / The Calvo assumption (Calvo, 1983) is widely used in the macroeconomic literature to model market frictions that limit the ability of economic agents to re-optimize their control variables. In spite of its virtues, the Calvo assumption also implies singular adjustment behavior at the firm level as well as a restrictive aggregation mechanism for the whole economy. In this study, I examine implications of the Calvo assumption for macroeconomic dynamics. To do so, I extend the Calvo assumption to a more general case based on the concept of the statistical hazard function. Two applications of this approach are studied in the DSGE framework. In the first essay, I apply this approach to a New Keynesian model, and demonstrate that tractability gained from the Calvo pricing assumption is costly in terms of inflation dynamics. The second essay estimates aggregate price reset hazard function using the theoretical framework constructed in the first essay, and shows that the constant hazard function implied by the Calvo assumption is strongly rejected by the aggregate data. In the third essay, I further explore implications of the empirically based hazard function for inflation persistence and monetary policy. I find that the empirically plausible aggregate price reset hazard function can generate simulated data that are consistent with inflation gap persistence found in the US CPI data. Based on these results, I conclude that the price reset hazard function plays a crucial role for generating inflation dynamics. The last essay applies the same modeling approach to a RBC model with employment rigidity. I find that, when introducing a more general stochastic adjustment process, the employment dynamics vary with a parameter, which determines the monotonic property of the hazard function. In particular, the volatility of employment is increasing, but the persistence is decreasing in the value of the parameter.

Wie sieht gute und alltagstaugliche Wohnarchitektur aus Sicht der Bewohner aus? 1.600 Mieterhaushalte in städtischen Wohnungen von acht deutschen Städten und eine kleine Kontrollgruppe von Eigentümern wurden in Face-To-Face-Interviews befragt. Ein Standardfragebogen wurde mit Computergraphiken und Animationen kombiniert. Der Blick gilt nicht der Gebäudehülle, sondern dem Inneren, dem Raumkonzept, der inneren Erschließung und der Freiraumzuordnung. Die Ergebnisse sind ein Leitfaden zum bedarfsgerechten Neu- und Umbau von städtischen Wohnungen für Bauherren und Planer. Die Architekturnutzer, überwiegend städtische Mieter, erhalten eine Stimme im Qualitätsdialog. Dem interessierten Laien wird eine Übersicht über vorhandene und mögliche Typologien des städtischen Wohnens vorgelegt. In den raumsoziologischen Diskurs wird ein Beleg dafür eingebracht, dass das Gebaute die Wohnweise und die Vorstellungswelt über richtiges Wohnen prägt. Die Studie ist ein Brückenschlag zwischen wohnsoziologischer Grundlagen- und anwendungsorientierter Marktforschung. Der Wunsch nach intelligenten Verbindungen von besonntem privaten Außenraum und Wohnung durchzieht die Ergebnisse wie ein grüner Faden. Es ist aus Sicht der Nutzer das Qualitätskriterium Nummer Eins und eine Kritik an vielen Defiziten städtischen Wohnens. Dem Planer zeigt es die Prioritäten eines bedarfsgerechten Neu- und Umbaus von städtischen Wohnungen auf. Das Gewohnte prägt das Gewünschte. Bewohnergruppen mit vergleichbaren soziodemographischen Merkmalen urteilen in Berlin anders als in Dresden oder Bochum. Der vorhandene Wohnungsbestand ist nicht nur das Ergebnis einer historisch spezifischen Wohnweise, sondern zugleich Prägestock und Begrenzung für das gelebte und das gewünschte Wohnen. / What has good housing architecture to be like, when the occupants are questioned.This leading question guidelines a survey among 1,600 tenants-households in eight German cities. Mainly designed as a post occupancy-evaluation the study contributes guidelines for a more userfriendly planning in urban housing construction and renovation. The survey was carried out by face-to-face interviews assisted by a standardized questionnaire, computer graphics and animation. It focuses on the inside, on floorplans, the idea of the floor plan, interior access and the combination of the interior and the private space outdoors. Space- sociology benefits from the results, as they prove, that the way of construction determines housing habits and housing needs. By means of a catalogue showing several common types of floor plans the occupants were consulted and got basic informations in order to distinguish, to evaluate and to choose among existing types of apartments. This work builds a bridge between basic resarch in housing sociology and user oriented market surveys. Unlike most studies on housing needs and demands, this survey does not operate by the fiction of a transparent line of products at housing markets and freedom of choice. Both, the design of the questionnaire and the shown types of floor-plans take the restrictions of the urban housing market into consideration as well as they mark the boundary of lower income demand and a limited knowledge about housing architecture. Many results underline the importance of intelligent links between interior and private space outdoors. Like a “green thread” running through the evaluation it’s a lesson about Number One quality issue from the view of users. We want, what we are used to. The existing housing stock engraves and restricts both, the historical residential manner and housing needs.

Proton therapy is a relatively new treatment modality for cancer, having recently been incorporated into hospitals in the last two decades. Although proton therapy has much higher start up and treatment costs than traditional methods of radiotherapy, it continues to expand in use today. One reason for this is that proton therapy has the advantage of a more precise localization of dose compared to traditional radiotherapy. Other proposed advantages of proton therapy in the treatment of cancer may lead to a faster expanse in its use if proven to be more effective than traditional radiotherapy. Therefore, much research must be done to investigate the possible negative and positive effects of using proton therapy as a treatment modality. In proton therapy, protons do account for the vast majority of dose. However, when protons travel through matter, secondary particles are created by the interactions of protons and matter en route to and within the patient. It is believed that secondary dose can lead to secondary cancer, especially in pediatric cases. Therefore, the focus of this work is determining both primary and secondary dose. In order to develop relevant simulations, the specifications of the treatment room and beam were based off of real-world facilities as closely as possible. Using available data from proton accelerators and clinical facilities, an accurate proton therapy nozzle was designed. Dose calculations were performed by MCNPX using a simple water phantom, and then beam characteristics were investigated to ensure the accuracy of the model. After validation of the beam nozzle, primary and secondary dose values were tabulated and discussed. By demonstrating the method of these calculations, the purpose of this work is to serve as a guide into the relatively recent field of Monte Carlo methods in proton therapy.

Die Lokalisierung von mRNA ist ein wichtiger regulativer Mechanismus in polarisierten Zellen und in frühen Embryonalstadien. Dort sind räumliche Muster maternaler mRNA für die korrekte Entwicklung der Körperachsen und die Spezifizierung der Keimzellen verantwortlich. Systematische Analysen dieser Prozesse wurden jedoch bisher limitiert durch einen Mangel an räumlicher und zeitlicher Auflösung von Einzelzell- Sequenzierungsdaten. Wir analysierten die Dynamik des räumlichen und zeitlichen Transkriptoms während frühen Embryonalstadien von Zebrafischen. Wir verbesserten Empfindlichkeit und Auflösung von tomo-seq und erfassten damit systematisch räumlich aufgelöste Transkriptome entlang der animal-vegetalen-Achse Embryonen im Einzell-Stadium und fanden 97 vegetal lokalisierte Gene. Außerdem etablierten wir eine Hochdurchsatz kompatible Variante der RNA-Markierungsmethode scSLAM-seq. Wir wendeten diese in Embryonen während der Gastrulation. Von den vegetal lokalisierten Genen waren 22 angereichert in Keimzellen, was eine funktionelle Rolle bei der Spezifizierung von Keimzellen nahelegt. Mit tomo-seq untersuchten wir die evolutionäre Konservierung der RNA-Lokalisierung zwischen Zebrafischen und gereiften Oozyten zweier Xenopus-Arten. Wir verglichen die lokalisierten Gene, suchten nach konservierten 3'UTR-Motiven, und fanden zum Teil überlappende Motive, was auf eine mögliche mechanistische Konservierung der Lokalisierungsmechanismen hinweist. Wir untersuchten auch RNA-Editierung von Adenin zu Inosin während der Embryonalentwicklung und in den Organen erwachsener Fische. In im Gehirn exprimierten Transkripten fanden wir 117 Editierstellen, die hauptsächlich für Ionentransporter kodieren und zum Teil zum Menschen konserviert sind. Die höchsten Editierraten konnten wir in Eierstöcken, Hoden und frühen Embryonen nachweisen, was auf eine mögliche Rolle bei der Regulierung der RNA-Stabilität hindeutet. / Subcellular localization of mRNA is an important regulatory mechanism in polarized cells. In early embryos of many species, spatial patterns of maternal mRNA are essential for the proper development of body axes and the specification of germ cells. These processes have been studied in zebrafish, but systematic analyses have been hindered by a lack of spatial and temporal information in single-cell RNA sequencing. We performed a spatial-temporal analysis of the zebrafish transcriptome during early embryonic development to systematically characterize localized mRNA and the fate of maternal transcripts until gastrulation stage. We enhanced sensitivity and resolution of the tomo-seq method and systematically acquired spatially-resolved transcriptomes along the animal-vegetal axis of one-cell stage zebrafish embryos, and found 97 genes to be localized vegetally. Furthermore, we established an in vivo and high-throughput compatible version of the single-cell RNA labeling method scSLAM-seq in gastrulation stage embryos. We followed localized transcripts until gastrulation and found transcripts of 22 of the vegetally localized genes enriched in primordial germ cells. We propose that these genes have a functional role in the early priming of the germ cell fate. To investigate the evolutionary conservation of vegetal RNA localization, we acquired tomo-seq datasets of mature oocytes of two xenopus species. We compared the pools of localized RNA and searched for conserved 3’UTR motifs. The resulting sets showed high similarity, possibly reflecting a mechanistic conservation of localization pathways. We also investigated RNA A-to-I editing during embryonic development and in organs of adult fish. Specifically, we identified 117 recoding editing sites in the brain that mainly encode for ion transporters and are partly conserved in humans. We detected the highest editing levels in ovary, testes and in early embryos, implicating a potential role in regulating RNA stability.

No description available.