Eine seneszente Zelle erfährt proteotoxischen Stress durch einen Prozess, der als “Seneszenz-assoziierter sekretorischer Phänotyp (SASP)” bezeichnet wird. SASP Faktoren involvieren vermehrte Produktion von Cytokinen und Chemokinen, die die Proteinhomöostase stören. Das führt zu Stress im Endoplasmatischen Retikulum (ER), Unfolded Protein Response (UPR) sowie erhöhter Autophagie in seneszenten Zellen. In dieser Arbeit zeigen wir, dass Therapie-induzierte Seneszenz (TIS), ausgelöst durch Adriamycin (ADR) oder Cyclophosphamid (CTX), zu einer erhöhten Expression von Alzheimer-assoziierten Genen führt. Das ist allerdings nur der Fall, wenn TIS mit SASP und Proteotoxizität korreliert. Außerdem zeigen wir, dass transgene Mäuse mit Alzheimer ebenso Eigenschaften von Seneszenz aufweisen. Mutationen in Genen wie Amyloid-Precursor-Protein (APP) und Presenilin 1 (PSEN1) erzeugen nicht nur Alzheimer, sondern verstärken auch Seneszenz. In APPPS1+/- transgenen Mäusen treten seneszente Zellen zusammen mit Amyloid β (Aβ) Plaques auf. Gleichfalls führt die Überexpression von APP in der Neuroblastom Zelllinie SH-SY5Y zu einer Erhöhung von Seneszenz-Markern, einschließlich ER Stress und Autophagie-assoziierten Lysosomen. Diese Beobachtungen implizieren einen Zusammenhang zwischen Seneszenz und Alzheimer. Wir präsentieren Proteotoxizität als gemeinsamen Nenner dieser beiden Pathologien. Allerdings kommen wir zu dem Ergebnis, dass APP und PSEN1 keine Regulatoren von TIS sind. APP trägt zur TIS-assoziierten Proteotoxizität bei, da ein knock-down von APP zu einer Reduzierung des ER Stress führt. Außerdem, können seneszente Zellen mittels Blockierung der APP Spaltung und somit Bildung von Aβ Plaques durch den Gamma-Sekretase Inhibitor Semagacestat, selektiv getötet werden. Das deutet auf ein senolytisches Potential hin. Allerdings hat Semagacestat keinen Einfluss auf die Expression von ER Stress Marker. Dies suggeriert, dass APP in Eμ-myc Lymphomen Aβ-unabhängig zu ER Stress beiträgt, während diese in Alzheimer eine zentrale Rolle in der Phathophysiologie spielen. / A senescent cell experiences proteotoxic stress in consequence to senescence-associated secretory phenotype (SASP). SASP factors involve increased production of cytokines and chemokines, which overload and disrupt protein homeostasis. This induces endoplasmic reticulum (ER) stress, unfolded protein response (UPR) as well as elevated autophagic-lysosomal burden in senescent cells, further promoting the formation of misfolded proteins. In this thesis we found, in Eμ-myc transgenic mice lymphomas, therapy-induced senescence (TIS) by Adriamycin or Cyclophosphamide treatment leads to an up regulation of genes involved in Alzheimer’s disease (AD). However, this is true only when TIS correlates with SASP, leading to proteotoxicity. On the other hand, we demonstrate that transgenic AD inflicted mice also present with features of senescence. Mutations of genes such as amyloid precursor protein (APP) and presenilin1 (PSEN1), not only engender AD, but also augment senescence. In APPPS1+/- transgenic mice, the senescent cells co-occur with amyloid β (Aβ) plaques. Likewise, in neuroblastoma cell line SH-SY5Y, APP overexpression results in upregulation of senescence markers, including markers for ER stress and autophagic-lysosomal burden. These observations imply that there is a crosstalk between senescence and AD. We demonstrate proteotoxicity as a common denominator between the two pathologies. However, we found that APP and PSEN1 are not regulators of TIS. APP contributes to proteotoxic stress associated with TIS as knocking down of APP leads to a reduction of ER stress. Inhibiting Aβ formation by targeting the processing of APP using Semagacestat- a gamma-secretase inhibitor, selectively targets senescent cells resulting in cell death. This indicates its senolytic potential. However, Semagacestat has no impact on the expression of ER stress markers. This suggested that in Eμ-myc lymphomas, APP contributes to ER stress in an Aβ-independent fashion, unlike in AD, where Aβ is considered central to its pathophysiology.

O presente trabalho tem como premissa investigar diretrizes, técnicas, métodos e padrões de acessibilidade existentes no contexto do governo eletrônico, via desktop e dispositivos móveis, de forma a identificar e analisar o que pode ser aplicado no processo de desenvolvimento para tornar acessíveis suas aplicações. Diante do crescimento da tecnologia e da necessidade de inclusão digital, o foco do trabalho destina-se a pessoa com deficiência, visando algo que possa atingir a todos de forma mínima. Assim, o trabalho teve como objetivo elaborar um conjunto de 35 recomendações de acessibilidade para aplicações móveis, adaptado do Modelo de Acessibilidade em Governo Eletrônico (e-MAG), padrão que consiste em recomendações específicas para o desenvolvimento de sítios e portais web do governo brasileiro. Após a adaptação das recomendações, foi realizado uma avaliação de 12 aplicativos do governo eletrônico, a avaliação foi uma inspeção por revisão de guidelines feita por um especialista, no caso, o próprio autor. A avaliação teve como objetivo validar as recomendações propostas, verificando a forma como o conteúdo e a informação são disponibilizados, isto é, se os aplicativos são efetivamente acessíveis. Pelos resultados obtidos, foi possível identificar que todas as aplicações apresentaram problemas de acessibilidade, prejudicando assim, a interação do usuário com a aplicação móvel. Também foi elaborado um questionário de acessibilidade formado pelas mesmas recomendações adaptadas, onde foi solicitado que pessoas voluntárias com deficiência o respondessem. Desta forma, o usuário atribuía um nível de relevância para cada recomendação do questionário, identificando ser muito ou pouco relevante para ele. Ao todo obtivemos 103 respostas, e de acordo com as análises realizadas, foi identificada variações de relevância entre os grupos e subgrupos de usuários que responderam o questionário, principalmente de pessoas com deficiência visual, nosso grupo mais expressivo neste trabalho, variando de usuários com visão próximo do normal a total cegueira. Com isso, foi possível elaborar uma lista de prioridades de recomendações de acessibilidade. A lista de prioridades tem como objetivo garantir que os principais requisitos de acessibilidade sejam implementados nas primeiras versões do software, ou seja, as recomendações consideradas mais relevantes pelos usuários, são consideradas prioritárias para o processo de desenvolvimento de aplicações móveis do governo eletrônico / The present work has as premise to investigate guidelines, techniques, methods and standards of accessibility existing in the context of e-government, via desktop and mobile devices, in order to identify and analyze what can be applied in the development process to make its applications accessible. Faced with the growth of technology and the need for digital inclusion, the focus of the work is aimed at people with disabilities, aiming at something that can reach everyone in a minimal way. The purpose of this study was to elaborate a set of 35 accessibility recommendations for mobile applications, adapted from the Electronic Government Accessibility Model (e-MAG), a standard that consists of specific recommendations for the development of websites and web portals of the Brazilian government . After adapting the recommendations, an evaluation of 12 e-government applications was carried out, the evaluation was a revision review of guidelines made by a specialist, in this case, the author himself. The evaluation aimed to validate the proposed recommendations, verifying how content and information are made available, that is, whether applications are effectively accessible. From the results obtained, it was possible to identify that all the applications presented accessibility problems, thus impairing the user interaction with the mobile application. An accessibility questionnaire was also prepared, which was made up of the same adapted recommendations, where volunteers with disabilities were asked to respond. In this way, the user assigned a level of relevance to each recommendation of the questionnaire, identifying it to be very or slightly relevant to it. In all, we obtained 103 responses, and according to the analyzes, we identified variations of relevance among the groups and subgroups of users who answered the questionnaire, mainly of people with visual impairment, our most expressive group in this study, ranging from users with vision close to normal to total blindness. This has made it possible to draw up a list of priorities for accessibility recommendations. The list of priorities aims to ensure that the main accessibility requirements are implemented in the first versions of the software, that is, the recommendations considered most relevant by the users, are considered as priorities for the process of developing e-government mobile applications

O presente trabalho tem como premissa investigar diretrizes, técnicas, métodos e padrões de acessibilidade existentes no contexto do governo eletrônico, via desktop e dispositivos móveis, de forma a identificar e analisar o que pode ser aplicado no processo de desenvolvimento para tornar acessíveis suas aplicações. Diante do crescimento da tecnologia e da necessidade de inclusão digital, o foco do trabalho destina-se a pessoa com deficiência, visando algo que possa atingir a todos de forma mínima. Assim, o trabalho teve como objetivo elaborar um conjunto de 35 recomendações de acessibilidade para aplicações móveis, adaptado do Modelo de Acessibilidade em Governo Eletrônico (e-MAG), padrão que consiste em recomendações específicas para o desenvolvimento de sítios e portais web do governo brasileiro. Após a adaptação das recomendações, foi realizado uma avaliação de 12 aplicativos do governo eletrônico, a avaliação foi uma inspeção por revisão de guidelines feita por um especialista, no caso, o próprio autor. A avaliação teve como objetivo validar as recomendações propostas, verificando a forma como o conteúdo e a informação são disponibilizados, isto é, se os aplicativos são efetivamente acessíveis. Pelos resultados obtidos, foi possível identificar que todas as aplicações apresentaram problemas de acessibilidade, prejudicando assim, a interação do usuário com a aplicação móvel. Também foi elaborado um questionário de acessibilidade formado pelas mesmas recomendações adaptadas, onde foi solicitado que pessoas voluntárias com deficiência o respondessem. Desta forma, o usuário atribuía um nível de relevância para cada recomendação do questionário, identificando ser muito ou pouco relevante para ele. Ao todo obtivemos 103 respostas, e de acordo com as análises realizadas, foi identificada variações de relevância entre os grupos e subgrupos de usuários que responderam o questionário, principalmente de pessoas com deficiência visual, nosso grupo mais expressivo neste trabalho, variando de usuários com visão próximo do normal a total cegueira. Com isso, foi possível elaborar uma lista de prioridades de recomendações de acessibilidade. A lista de prioridades tem como objetivo garantir que os principais requisitos de acessibilidade sejam implementados nas primeiras versões do software, ou seja, as recomendações consideradas mais relevantes pelos usuários, são consideradas prioritárias para o processo de desenvolvimento de aplicações móveis do governo eletrônico / The present work has as premise to investigate guidelines, techniques, methods and standards of accessibility existing in the context of e-government, via desktop and mobile devices, in order to identify and analyze what can be applied in the development process to make its applications accessible. Faced with the growth of technology and the need for digital inclusion, the focus of the work is aimed at people with disabilities, aiming at something that can reach everyone in a minimal way. The purpose of this study was to elaborate a set of 35 accessibility recommendations for mobile applications, adapted from the Electronic Government Accessibility Model (e-MAG), a standard that consists of specific recommendations for the development of websites and web portals of the Brazilian government . After adapting the recommendations, an evaluation of 12 e-government applications was carried out, the evaluation was a revision review of guidelines made by a specialist, in this case, the author himself. The evaluation aimed to validate the proposed recommendations, verifying how content and information are made available, that is, whether applications are effectively accessible. From the results obtained, it was possible to identify that all the applications presented accessibility problems, thus impairing the user interaction with the mobile application. An accessibility questionnaire was also prepared, which was made up of the same adapted recommendations, where volunteers with disabilities were asked to respond. In this way, the user assigned a level of relevance to each recommendation of the questionnaire, identifying it to be very or slightly relevant to it. In all, we obtained 103 responses, and according to the analyzes, we identified variations of relevance among the groups and subgroups of users who answered the questionnaire, mainly of people with visual impairment, our most expressive group in this study, ranging from users with vision close to normal to total blindness. This has made it possible to draw up a list of priorities for accessibility recommendations. The list of priorities aims to ensure that the main accessibility requirements are implemented in the first versions of the software, that is, the recommendations considered most relevant by the users, are considered as priorities for the process of developing e-government mobile applications

Made available in DSpace on 2017-07-10T16:28:03Z (GMT). No. of bitstreams: 1 Denila Coelho.pdf: 3007094 bytes, checksum: 28befae07ccd55db5b2dbb6206ebd27d (MD5) Previous issue date: 2015-08-28 / The working class organization throughout history played a key role in winning rights and better work conditions. In order to better understand the struggle story and organization of Paraná education workers, we carried out this research aiming to know and understand the influence and impact caused by the Training School of APP-Union in political and trade union training of the Trade Union in Francisco Beltrão Center - NSFB educators, from 2007 to 2014. The research method was dialectical historical materialism, and the research sources were bibliographies, documents produced by APP-Union, namely, articles in the institutional website, August 30 newspaper, videos, training books, projects and study reports, registration forms and training attendance lists, NSFB minutes, status of the entity, data taken from the Ministry of Education website - SEED, Paraná and Brazil legislation, as well as questionnaires answered by fifty NSFB educators. The union political education was shaped in an important tool for knowledge socialization, consciousness expansion and education workers organization in the State of Paraná. We pointed out the limits and possibilities of the training offered by the School of Education of APP-Union and from the results we present a proposal of training for NSFB. We conclude that despite the difficulties faced by the school, the experience was/is an important work that needs to be intensified to reach the largest possible number of educators. We understand that the training of teachers is strategic because these professionals play an important task which is schooling, that is, they act directly on the education of the whole society. We emphasize that only an education designed by the working class is capable of enabling conditions for the realization of social change long sought by workers. / A organização da classe trabalhadora ao longo da história teve papel fundamental na conquista de direitos e de melhores condições de trabalho. No intuito de conhecer melhor a história de luta e a organização das/os trabalhadoras/es em educação do Paraná, realizamos esta pesquisa, com o objetivo de conhecer e entender as influências e os impactos provocados pela Escola de Formação da APP-Sindicato na formação política e sindical das/os educadoras/es do Núcleo Sindical de Francisco Beltrão - NSFB, no período de 2007 à 2014. O método de pesquisa utilizado foi o materialismo histórico dialético e as fontes de investigação foram: bibliografias; documentos produzidos pela APP-Sindicato, sendo eles: matérias no site institucional, jornal 30 de Agosto, vídeos, cadernos de formação, projetos e relatórios de estudos, fichas de inscrição e listas de presença da formação, atas do NSFB, estatuto da entidade; dados retirados do site da Secretaria de Estado da Educação SEED, legislação do Estado e do Brasil, além de questionários respondidos por cinquenta educadoras/es do NSFB. A formação política sindical se configurou em um importante instrumento de socialização de conhecimento, ampliação de consciência e organização das/os trabalhadoras/es em educação no Estado do Paraná. Apontamos os limites e possibilidades da formação oferecida pela Escola de Formação da APP-Sindicato e a partir dos resultados apresentamos uma proposta de formação para o NSFB. Concluímos que apesar das dificuldades enfrentadas pela escola, a experiência foi/é um importante trabalho que precisa ser intensificado para que atinja o maior número possível de educadoras/es. Entendemos que a formação das/os educadoras/es é estratégica, pois estes profissionais desempenham uma tarefa importante que é a escolarização, ou seja, atuam diretamente na educação de toda a sociedade. Enfatizamos que somente uma educação pensada pela classe trabalhadora será capaz de possibilitar condições para a realização da transformação social tão almejada pelas/os trabalhadoras/es.

AudioApps är ett företag som tillverkar mobilapplikationer till bland annat museer och gallerier. Deras företagsidé är att göra museibesök roligare för besökare genom mobila audioguider. I den här undersökningen studeras utvecklingen av en sådan tjänst som tagits fram för Nationalmuseum, ett av Sveriges ledande museer. Syftet med studien har varit att undersöka hur AudioApps kan hjälpa Nationalmuseums kunder att attrahera fler besökare, samt hur AudioApps egna varumärke kan utnyttjas i eller påverka tjänsterna. Studien avgränsas av Nationalmuseum som ensam representant för hela den bransch som AudioApps vill jobba med och av den enskilda målgrupp som undersökts. Målgruppen består av personer mellan 20-30 år som anses vara ”något intresserade” av kultur och konst. De frågor som har undersökts är hur AudioApps varumärkeskommunikation kan formas för att attrahera fler besökare till Nationalmuseum och hur AudioApps tjänst kan formas för att göra ett museibesök mer attraktivt för den valda målgruppen. Kvalitativa metoder med strukturerade intervjuer och tester, efter en förberedande fallstudie av Nationalmuseum, har använts för att undersöka frågeställningarna. Det framgick av undersökningen att målgruppen gärna ville ha hjälp av extra information vid ett museibesök. Kostnad och frihet var viktiga aspekter angående sådan extra information. Att öka attraktionen för ett museibesök var också något som kunde förbättras, som målgruppen själv gärna ville. Det visade sig att ett problem låg i bristen på tydlig marknadsföring. Den tjänst som testades på målgruppen togs emot positivt. Den uppfyllde kraven på kostnad, smidighet och tillät en tydligare kommunikation mellan museet och dess besökare. Det framgick även av testerna att AudioApps varumärkeskommunikation förhöjde den visuella upplevelsen av tjänsten. / AudioApps produces mobile applications to use at museums and galleries. As a company, they want to make a visit to a museum more attractive for visitors through mobile audio systems. In this investigation the development of such an application which has been made for Nationalmuseum, one of Swedens top museums, was studied. The purpose of this thesis was to study how AudioApps can help their customers attract more visitors and how AudioApps own brand can be of use and affect the products. The thesis is limited by Nationalmuseum as a sole representative of the industry AudioApps aims to address and by the target group that’s chosen for the study. The target group consists of people in the age of 20 to 30 years old and who are "somewhat interested" in arts and culture. The main questions of this thesis are how AudioApps’ brand communication can be formed to attract motre visitors to Nationalmuseum and how AudioApps’ product can make a museum visit more attractive. Qualitative methods, which started off with a case study of Nationalmuseum, has been used for this study, with mainly structured interviews and tests. The study has shown that the target group enjoys extra information during a visit. Cost and freedom were important aspects regarding that kind of information. Making a visit in the first place was something that needed improvement, which the target group itself wanted. The problem was the communication which needed to be more clear. The product was well received by the target group during tests. It met the requirements of cost, freedom and allowed a direct and clear communication between the museum and its customers. The tests also showed that AudioApps’ brand communication enhanced the visual experience of the product.

The analysis of acute phase protein serum amyloid A (SAA) has recently been brought into clinical use in veterinary medicine. Some of the difficulties with incorporating the SAA method in clinical practice have been the expensive and rather large equipment required for the method. Due to these difficulties only larger clinics can afford to use the SAA analysis. The company Equinostic has recently developed a smaller instrument that costs one-tenth of a larger instrument. The instrument is named EVA1 and has so far only been used to analyze SAA in horses. The aim of this study was to investigate if the EVA1 instrument could be used to analyze SAA in cats. This study included 24 serum samples from cat, which were first analyzed twice on the EVA1 instrument and then sent to the Strömsholm Referral Animal Hospital in Sweden where they reanalyzed the samples using a validated reference method. Both instruments are based on an immunoturbidimetric assay. The correlation between the two instruments was good (r=0.97) but the EVA1 instrument showed constantly lower results than the reference method. The difference between the duplicates when analyzed on the EVA1 instrument was larger than expected. The conclusion is that EVA1 could be used to analyze SAA in cats. However, before it could be used clinically in veterinary practice an extended study is recommended.

Seit dem 18. März diesen Jahres steht im Apple App-Store die SLUB-Katalog-App zum Download zur Verfügung. Am 26. April folgte die Android-Version bei Google Play. Wir sind stolz, dass die SLUB-App innerhalb weniger Wochen knapp dreitausend Mal herunter geladen wurde und die SLUB damit dem Trend zur mobilen und jederzeit verfügbaren Nutzung von Wissen Rechnung tragen konnte.

Mobile applications are becoming more and more important in the daily life and offer interesting and completely new aspects to the field of cartography. The technological possibilities provided by computer science have had a large impact on cartography and therefore cartographers must be familiar with the development of software to present geographic information and make it accessible. With the recent rise of mobile devices like smartphones, it is inevitable that this has to include mobile platforms as well. The main focus of this Bachelor thesis is on the conceptual design and implementation of tutorials to teach the development of mobile applications or 'apps' for the Android platform. The tutorials are going to be part of exercises in a practical course that accompanies the lecture 'Mobile Cartography' held at the Institute of Cartography at the Dresden University of Technology. Five exercises have been created covering key aspects of Android programming with Java. The theoretical part of this thesis starts with providing definitions of important terms of mobile computing and mobile cartography that will play an important role throughout the thesis. After that possibilities of interaction with mobile and web applications are introduced and discussed briefly. Location-based services and their usage in the context of social networks are discussed in the following section before the sensors of mobile devices are analyzed. A brief discussion of the potential for augmented reality applications is also given. The following second section deals with mobile software platforms and focuses on the Android operating system for mobile devices. Within this section, the features of the Android framework are described and some specific characteristics of software development are explained. The third section is all about the conceptual design and implementation of the tutorials. Considering the requirements for the practical course and different aspects of learning, the pedagogical approach is described. Additionally the ECLASS model which has evolved in the context of E-Learning is explained as it provided the structure of the exercises. After that, the content of the five exercises which include basics, layout, internal interaction of components, database access and the integration of maps is reflected. Finally, a brief outlook on further possible exercises is given.

App 內購買(In-App Purchase, IAP)已成為現今App 營收的主要模式，而過 去針對此一現象的研究大多集中於遊戲類的App，較少針對其他類型App(工 具、資訊、社交)進行探討。 本研究先利用文獻歸納的方式，整理出可能影響使用者App 內購買的因 素，再透過深度訪談法，針對曾經進行過App 內購買的使用者進行訪問，歸納 出App 使用動機、販售商品與App 的互補性與口碑情緒三個因素會影響使用者 對商品的知覺價值與購買意圖，並依此建構本研究的研究模型，並進行實驗設 計。在實驗中，本研究挑出四種不同類型的App，並以高低互補性、正面口碑 的有無為操弄變項，搭配App 使用動機來搜集資料並進行分析。 研究結果發現：(1) 部分App 使用動機會對知覺價值與購買意圖有顯著影響。 (2) App 與商品的互補性對於知覺價值與購買意圖皆有顯著影響。(3) 口碑對於 商品的知覺價值有顯著影響，但是對於購買意圖沒有顯著影響。顯示IAP 商品 是否能與App 有效整合，為App 帶來更多的價值，才是影響使用者購買意圖的 最大因素。 / In-App purchase(IAP) has become the main revenue model in App market. Previous studies on this issue are mostly focused on game Apps, and rarely explored other types of Apps such as productivity tools, information or social networking tools. In this study, we use literature analysis and in-depth interviews to find factors that may affect users’ in-app purchase intention. User motivation, product complementarity, and word-of-mouth are found to affect users’ perceived value and in-app purchase intention. We developed a theoretical model and designed an experiment to test the model. In the experiment, we chose four different types of Apps, designed with different levels of complementarity and different sentiment of word-of-mouth (user comments) as the treatment. The results indicated that: (1) Certain motivations significantly affected the perceived value and purchase intention of IAP products. (2) Complementarity between IAP products and the App significantly affected the perceived value and purchase intentions of IAP product. (3) word-of-mouth significantly affected perceived value but not affecting the purchase intention of IAP products.

No description available.