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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
241

Eletroforese das proteínas séricas e urinárias de cães com erliquiose subclínica

Coelho, Stefanie Bertti [UNESP] 02 July 2015 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:02:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-02. Added 1 bitstream(s) on 2015-10-06T13:19:18Z : No. of bitstreams: 1 000848480.pdf: 612627 bytes, checksum: 36cf043f291e693b992eabd762503b7b (MD5) / A erliquiose é uma hemoparasitose que acomete cerca de 20 a 30% dos cães atendidos nas clínicas veterinárias do Brasil, podendo levá-los ao óbito. Predomina nas regiões mais quentes do planeta devido ao fato de seu hospedeiro intermediário, um artrópode, prevalecer nos locais de altas temperaturas. Em função da gravidade da enfermidade para os animais e ao potencial zoonótico da doença, estudos envolvendo as possíveis alterações renais são essenciais para o diagnóstico e prognóstico da doença. Neste ensaio, objetivou-se avaliar as proteínas séricas e urinárias de cães com erliquiose na fase subclínica, por meio da técnica de eletroforese em gel de poliagrilamida contendo dodecil sulfato de sódio (SDS-PAGE). Os animais foram devidamente distribuídos em grupo controle (GC), 18 cães saudáveis e grupo doente (GD), integrado por 17 cães acometidos por erliquiose subclínica, sendo que o diagnóstico da infecção foi confirmado ou excluído levando-se em conta a associação de exames clínicos, sorológicos e PCR. Os resultados mostraram que os cães na fase subclínica da erliquiose apresentaram alterações laboratoriais como, trombocitopenia, hiperglobulinemia com hipoalbuminemia compensatória e aumento de proteínas totais, além de anemia. A partir do traçado eletroforético sérico, concluiu-se que os cães infectados na fase subclínica apresentaram elevação na concentração de transferrina, IgG de cadeia pesada, haptoglobina, alfa 1-glicoproteína ácida e proteína de peso molecular 23 kDa. A análise do traçado eletroforético urinário dos animais experimentais, associada com o resultado da UPC, o qual revela proteinúria limítrofe nos indivíduos doentes, demonstraram que existem lesões renais, tanto em nível glomerular quanto tubular, em cães com erliquiose na fase subclínica, entretanto, mais estudos se fazem necessários para esclarecer e detalhar melhor as referidas lesões / Ehrlichiosis is a hemoparasitosis that affects around 20 to 30% of dogs treated at veterinary clinics in Brazil, which may lead the animal to death. It predominate in the warmer regions of planet because of its intermediate host, an arthropod, that lives in high temperature regions. Due to the severity of the disease to animals and its zoonotic potencial, studies involving the possibles renal disorders are essential for the diagnosis and patient's prognosis. In this trial aimed to evaluate serum and urinary proteins of dogs with ehrliquiosis in the subclinical stage, by electrophoresis in poliacrilamida gel containing sodium dodecyl sulfate (SDS-PAGE). The animals were divided into control group (CG), 18 health dogs and sick group (SG), the last one with 17 dogs with diagnosis of erlichiosis subclinical, and the diagnosis of infection was conformed or excluded by association of clinical exams, serological tests and PCR. The results showed that dogs on the subclinical stage of ehrlichiosis had laboratory changes as thrombocytopenia, hyperglobulinemia associated with compensatory hypoalbuminemia and increased total protein, and anemia. From the serum electrophoresis, it was concluded that the infected dogs in subclinical phase had increased concentration of transferrin, the heavy chain of IgG, haptoglobin, alpha 1-acid glycoprotein and protein molecular weight 23 kDa. The analysis of urinary electrophoretic trace of the experimental animals, the result associated with the UPC, which shows borderline proteinuria in diseased dogs, showed that there renal lesions, both as tubular glomerular level, in dogs with erliquiosis in the subclinical stage, however, more studies are needed to clarify and better detail these injuries
242

Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda

Contin, Catarina Micali [UNESP] 02 July 2015 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:02:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-02. Added 1 bitstream(s) on 2015-10-06T13:19:16Z : No. of bitstreams: 1 000848474.pdf: 572372 bytes, checksum: 3047ed378c8d8d3cc0c615fe38af8715 (MD5) / A erliquiose canina é uma das hemoparasitoses mais frequentes entre os cães domésticos e apresenta alta prevalência nas regiões de clima tropical. Apesar das inúmeras investigações e avanços na compreensão da patogênese e diagnóstico da referida doença, pouco se sabe a respeito das alterações glomérulo-tubulares de cães acometidos por erliquiose. No presente estudo objetivou-se associar o perfil eletroforético das proteínas sanguíneas e urinárias com as possíveis alterações glomérulo-tubulares encontradas em cães com erliquiose na fase aguda. O Grupo Controle (GC) foi integrado por 18 cães saudáveis e o Grupo Doente (GD), por 24 animais. Ambos de qualquer gênero, raça e idade. O protocolo experimental incluiu avaliação das condições clínicas de cães sadios e daqueles acometidos pela doença, neste último caso com base nos sinais clínicos e presença de mórula em esfregaços sanguíneos de ponta de orelha. Dos animais controle e daqueles enfermos, foram obtidas amostras de sangue periférico e urina para análises eletroforéticas das proteínas pela técnica de eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE). Foram selecionadas 11 frações proteicas no traçado densitométrico dos soros sanguíneos, cujos pesos moleculares variaram entre 23 e 139 kDa (kilodáltons). Os resultados mostraram que algumas proteínas séricas de fase aguda se alteram nos cães com erliquiose na fase aguda. Observou-se diferença significativa entre os grupos estudados na concentração sérica de transferrina, haptoglobina, IgG (cadeia leve) e albumina, sendo que somente esta última apresentou níveis inferiores no Grupo Doente. A análise eletroforética da urina exibiu um número superior de frações proteicas excretadas pelos cães com erliquiose na fase aguda (295), quando comparados àqueles encontrados nos cães do GC (174). Foram encontradas proteínas de alto (>60 kDa) e baixo... / Canine ehrlichiosis is one of the most frequent haemoparasitosis between domestic dogs and has high prevalence in tropical regions. Despite the innumerous investigations and progresses in the pathogenesis comprehension and diagnosis of the disease, little is known on the glomerulo-tubular alterations of dogs stricken by ehrlichiosis. The aim of this study was to associate the electrophoretic profile of blood and urinary proteins with the possible glomerulo-tubular alterations found in dogs with acute-phase ehrlichiosis. The Control Group (CG) was formed by 18 healthy dogs and the Sick Group (SG), by 24 animals, both groups with unspecified genders, races and ages. The experimental protocol included assessment of the dogs' clinical conditions, and regarding the sick ones, based on the clinical signs and the presence of morula in the ear-tip blood smears. From the animals of both groups, peripheral blood and urine samples were obtained for protein electrophoretical analysis through the polyacrylamide gel with dodecyl sodium sulfate (SDS-PAGE) electrophoresis technique. Eleven protein fractions were selected in the blood sera densitometry trace, with molecular weights ranging from 23 to 123 kDa (kilodaltons). The results showed that some acute-phase proteins change in dogs with acute-phase ehrlichiosis. Significant difference between the studied groups was observed in serum concentrations of transferrin, haptoglobin, IgG (light chain) and albumin, the latter with lower levels in the Sick Group. The electophoretical analysis of urine showed a larger number of protein fractions excreted by dogs with acute-phase ehrlichiosis (295), when in comparison with the ones in the CG (174). High (>60 kDa) and low (<60 kDa) molecular weight proteins were found in the sick dogs' urine, suggesting that the disease in its acute phase causes lesions in both glomeruli and tubules, respectively
243

Uso de viscum album no ponto de acupuntura VG14 como terapia adjuvante à mastectomia radical em cadelas com neoplasias mamárias

Figueiredo, Renata Nogueira [UNESP] 19 August 2014 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:03:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-08-19. Added 1 bitstream(s) on 2015-10-06T13:19:01Z : No. of bitstreams: 1 000849244.pdf: 624171 bytes, checksum: 87b755a945d322c151256c67ed4aabbe (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Acupuncture should be seen as an adjuvant treatment modality, because it improves the quality of life of patients with cancer, in addition to controlling these metabolic changes. Concomitantly, Viscum album extract used in anthroposophical medicine can be used to cause cytotoxicity and apoptosis of tumor cells and therefore has been widely applied. The data showed an increase of these effects when applied at point Governor Vessel. (VG14), one of the immunity points. For decades numerous therapies have been used to combat cancer, wherein the surgery still is the primary form of treatment protocols which are set according to the type and place of occurrence thereof. Other therapies are also applied as chemotherapy employing cytotoxic agents, but they produce side effects and adverse effects. We conclude that adjuvant therapy made the bitches presented lower values of globulins, calcium and FA to assist in preventing the development of paraneoplastic syndromes. However, further research should be conducted to more accurately assess the beneficial effects of using these complementary therapies, as this chapter shows
244

Avaliação da quimioterapia metronômica em carcinomas mamários de cadelas por imunomarcações

Mendes, Analy Ramos [UNESP] 15 August 2014 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:03:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-08-15. Added 1 bitstream(s) on 2015-10-06T13:18:49Z : No. of bitstreams: 1 000848999.pdf: 1544826 bytes, checksum: 3fb73afedffc7650ac4620d6a79c29e9 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / The canine mammary tumor (CMT) is the neoplasia that most commonly affects not spayed bitches. Therapy for CMT is a challenge since there are not described effective treatments for high-grade tumors. A therapeutic option that slows down tumor angiogenesis is metronomic chemotherapy (MC). Quantification of tumor angiogenesis has been proposed by measuring the vascular endothelial growth factor (VEGF) and microvessel density (MVD). Thus, in this study, the response of canine mammary carcinomas by the MC was evaluated by the measurement of MVD and tissue VEGF detection, apoptotic index (AI) and cell proliferation index (PI). Twenty-eight dogs with malignant CMT, equally distributed into a control group (CG) treated with mastectomy and a treated group (TG) treated with MC (cyclophosphamide 15mg/m2 and piroxicam 0.3 mg/kg) both orally and daily for 28 days followed by mastectomy. Mammary tumors were classified and graded histologically. MVD, tissue VEGF, AI and PI of all malignancies were obtained by immunostaining. The analysis showed statistical difference between groups in MVD and AI (p <0.05), showing quantitative reduction in tumor microvasculature and increase in tumor cells apoptosis. Based on this result, we can affirm that MC has antiangiogenic and proapoptotic effects in mammary carcinomas of bitches and can be used as a new therapeutic option
245

Efeito das toxinas urêmicas guanidina e ácido guanidinoácetico sobre o metabolismo oxidativo e apoptose em neutrófilos de cães

Pereira, Priscila Preve [UNESP] 17 January 2014 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:03:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-17. Added 1 bitstream(s) on 2015-10-06T13:18:35Z : No. of bitstreams: 1 000848874.pdf: 475629 bytes, checksum: 8f3083521bd5dbb20833e54b2e295e0b (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / It has been recently reported that in canine chronic renal failure (CRF) there occurs oxidative stress, and that neutrophil dysfunction is associated with the increase of apoptosis. Among the many uremic toxins found in higher concentrations due to CRF, there is evidence - in humans - that guanidines inhibit the oxidative metabolism of neutrophils, affecting their bactericidal function. In this study, a systematic review of the relationship between guanidine compounds and neutrophils in dogs was undertaken. It was found that the literature on this subject is scarce and conflicting, which prompted this investigation of the relationship between the plasmatic concentration of guanidine, the production of superoxide, and the apoptosis of neutrophils in dogs with CRF. Morever, the effect of the isolated compound guanidine acetic acid (GAA) on the production of superoxide and on neutrophil apoptosis in healthy dogs was investigated in vitro. It was possible to verify that the large increase of plasmatic guanidine observed in dogs with CRF is not associated with the altered oxidative metabolism and the apoptosis of the neutrophils. The GAA inhibited the oxidative metabolism of the neutrophils in healthy dogs without affecting the viability of these cells
246

Caracterização das espécies de leishmania em sangue periférico de cães por PCR-RFLP NA área endêmica de Bauru/SP

Sanches, Letícia da Cruz [UNESP] 16 June 2014 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:03:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-06-16. Added 1 bitstream(s) on 2015-10-06T13:18:30Z : No. of bitstreams: 1 000849239.pdf: 306516 bytes, checksum: cab162ea96c4456d9e47531798b2c3eb (MD5) / Leishmaniasis is a major public health problem in the world. The protozoa of the genus Leishmania has worldwide distribution and epidemiology of the disease depends on the characteristics of the parasites. Leishmaniasis are divided into visceral leishmaniasis and cutaneous. The dog plays a key role in the transmission of L. infantum to humans and in the epidemiology of the disease. Due to the adaptation of Leishmania to new hosts or vectors is important to know the current etiologic agent in dogs. Molecular techniques have been used for the diagnosis of leishmaniosis. The PCR-RFLP detects and distinguishes the different species of the parasite. The objective of this study was to identify the Leishmania species found in 103 samples of peripheral blood of dogs naturally infected with this protozoan, the city of Bauru - SP. For the diagnosis of leishmaniosis was determined by parasitological examination, indirect ELISA and PCR was performed. The determination of Leishmania species the DNA amplified intergenic region ITS1 was digested with the restriction enzyme HaeIII. Positive samples for Leishmania ssp. showed an identical restriction profile of L. amazonensis in 77/103 samples, 17/103 were similar to L. infantum, and 09/103 were mixed profile. In conclusion, we identified L. amazonensis greater number of dogs than L.infantum in Bauru city, SP
247

Avaliação da administração de bupivacaína à altura da primeira vértebra lombar, sobre parâmetros cardiorrespiratórios, bloqueios sensitivo e anestésico, em cadelas submetidas à ovariohisterectomia

Silva, Paloma do Espírito Santo [UNESP] 21 July 2015 (has links) (PDF)
Made available in DSpace on 2015-10-06T13:03:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-21. Added 1 bitstream(s) on 2015-10-06T13:18:22Z : No. of bitstreams: 1 000849618.pdf: 1481058 bytes, checksum: 223cf15b98a011bfbc3974f15475fffa (MD5) / A anestesia epidural é uma técnica disseminada, tanto pela facilidade de execução quanto pela segurança e conforto pós-operatório para o paciente. Entretanto, a administração de fármacos em vértebras craniais à sétima vértebra lombar com o uso de cateter epidural é pouco estudada. A presente pesquisa utilizou 16 cadelas, distribuídas aleatoriamente em 2 grupos, os quais se diferenciaram pela dose epidural de bupivacaína, 1mg/kg (G1) e 2 mg/kg (G2). A medicação pré anestésica foi feita com butorfanol e após a indução com etomidato, o cateter epidural foi posicionado à altura da primeira vértebra lombar. Após 30 minutos, foi administrada a bupivacaína e o bloqueio sensitivo foi avaliado durante 30 minutos. Em seguida, foi realizada a cirurgia de ovariohisterectomia eletiva, durante a qual, a eficácia do bloqueio anestésico foi avaliada. Também foram avaliados parâmetros cardiovasculares, ventilométricos e hemogasométricos. Os resultados das variáveis paramétricas foram analisados pelo software SAS 9.4 (2010) e não houve diferenças relevantes entre os grupos em relação aos parâmetros fisiológicos estudados. OS resultados referentes ao bloqueio sensitivo demonstram que o G2 apresentou bloqueio superior para todas as variáveis. Do mesmo modo, o G2 apresentou melhor bloqueio anestésico durante o transcirúrgico. Conclui-se que as duas doses de bupivacaína administradas à altura da primeira vértebra lombar não interferem significativamente nos parâmetros cardiovasculares, ventilométricos e hemogasométricos. Além disso, a dose de 2mg/kg determina melhor bloqueio sensitivo e anestésico que a dose de 1mg/kg nestas condições experimentais. A dose de 2 mg/kg permitiu a ovariohisterectomia em cadelas / The epidural anesthesia is a widespread technique, presenting easy execution, safety and postoperative comfort for the patient. However, administration of drugs cranial to the seventh lumbar vertebrae using an epidural catheter is little studied. This study used 16 bitches that were separated randomly into 2 groups, which differed by epidural bupivacaine dose, 1 mgkg-1 (G1) or 2 mgkg-1 (G2). They were premedicated with butorphanol and and after the induction with etomidate, the epidural catheter was positioned at the first lumbar vertebra. After 30 minutes, bupivacaine was administered and sensory block was evaluated for 30 minutes. The anesthetic block was evaluated during an ovariohisterectomy elective surgery. The cardiovascular, ventilometric and blood gas parameters were also evaluated. The results of parametric variables were analyzed by SAS 9.4 software (2010). There were few differences between the groups in relation to physiological parameters but they remained near the normal range for the specie. The analysis of sensory block showed that G2 had better blocking for all variables. Similarly, the G2 showed better anesthetic block during the surgery. It concludes that the two bupivacaine doses administered up to the first lumbar vertebra, do not interfere significantly in the cardiovascular, ventilometric and blood gas parameters. Furthermore, the dose of 2mgkg-1 results in a better anesthetic and sensitive block than the dose of 1mgkg-1 in these experimental conditions. The dose of 2mgkg-1 was effective to ovariohysterectomy in bitches
248

Consumo de amido e proteína, excreção de oxalato e características da urina de gatos alimentados com ração seca

Pedreira, Raquel Silveira [UNESP] 17 July 2015 (has links) (PDF)
Made available in DSpace on 2015-12-10T14:22:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-17. Added 1 bitstream(s) on 2015-12-10T14:28:50Z : No. of bitstreams: 1 000855341.pdf: 1074140 bytes, checksum: 98940109d9d06c8c7f2bf99d2642cb2b (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Gatos são animais carnívoros, com elevada necessidade de aminoácidos. O aproveitamento de carboidratos por esses animais já foi estudado, contudo existem informações, relacionadas ao metabolismo deste nutriente, que ainda não são bem esclarecidas para a espécie. O objetivo deste estudo foi avaliar o efeito do consumo de dietas com diferentes relações entre amido e proteína sobre a formação e excreção do oxalato e o índice de supersaturação relativa da urina para oxalato de cálcio (SSR OxCa). Foram avaliados alimentos extrusados com diferentes proporções de amido (CHO) e Proteína (PB): Dieta A 47 com 47% de CHO e 25% de PB; dieta A 32 com 31,9% de CHO e 38% de PB; dieta A 19 com 19,2% de CHO e 52% de PB e dieta A 8 com 8,3 % de CHO e 62% de PB (na matéria seca). O estudo foi organizado em 4 blocos de 18 dias, cada um com quatro rações e oito gatos, totalizando 32 gatos e oito repetições por ração. Os gatos permaneceram em gaiolas metabólicas durante 10 dias de adaptação à dieta, seguidos de sete dias de coleta total de urina e fezes. Durante os dois primeiros dias de coleta a urina foi recolhida em garrafas com 1mL de H2SO4 (1N) e nos cinco dias seguintes em garrafas com 100mg de timol. Na urina produzida em cada período de 24 horas foi aferido o volume, densidade e pH. A urina de 5 dias de cada gato (colhida com timol) foi homogeneizada, sendo determinados os teores de cálcio, fósforo, cloro, enxofre, magnésio, sódio, potássio, citrato, oxalato, ácido úrico, creatinina e ureia. Na urina acidificada foram determinados o nitrogênio e energia bruta. Amostras de sangue para obtenção do plasma foram colhidas no 18º dia de cada período, sendo analisadas para oxalato e creatinina. Os alimentos e as fezes foram avaliados para matéria seca, matéria mineral, amido, fibra bruta e energia bruta. Com base nesses resultados foi calculada a SSR OxCa e a excreção fracionada de oxalato. Os resultados obtidos... / Cats are carnivores and have high amino acids requirements. The use of carbohydrates by these animals has been evaluated; however, there are some information that is not well understood. The objective of this study was to evaluate the effect of consumption of diets with different relations between starch and protein on the formation and excretion of oxalate, and the relative supersaturation index of calcium oxalate in urine (SSR OxCa). Four diets were extruded with different starch (CHO) and protein (CP) ratios: diet 47 with 47% of CHO and 25% of CP; diet 32 with 31.9% of CHO and 38% of CP; diet 19 with 19.2% of CHO and 52% of CP; and diet 8 with 8.3% of CHO and 62% of CP (dry matter basis). The experiment was organized in 4 blocks of 18 days; there were 4 diets and 8 cats in each block, resulting in eight animals per diet. Animals were kept in metabolic cages during 10 days for diet adaptation followed by 7 days of total urine and feces collection. Urine was collected in bottles with 1 ml of H2SO4 (1N) in the first two days of the collection period and the last five days in bottles with 100 mg of thymol. Volume, density and pH of urine were measured each 24 hours during the whole experiment. Urine harvested with thymol were homogenized and analyzed for calcium, phosphorus, chlorine, sulfur, magnesium, sodium, potassium, citrate, oxalate, uric acid and creatinine. In the acidic urine were determined nitrogen and gross energy. Blood samples were taken at 18th day of the experiment and evaluated for oxalate and creatinine. Food and feces were evaluated for dry matter, ash, starch, crude fiber and gross energy. Based on the results SSR OxCa and fractional excretion of oxalate were calculated. Data were submitted to analysis of variance and when significant differences were detected in the F test, multiple comparisons were performed using polynomial contrasts (P <0.05). Starch inclusion up to 47% did not restrict the diet consumption by ... / FAPESP: 2013/15766-9
249

Avaliação da resposta imune celular de sangue periférico e medula óssea pela técnica de imunocitoquímica em cães vacinados para Leishmaniose visceral canina

Paiva, Milla Bezerra [UNESP] 14 August 2012 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:25:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-08-14Bitstream added on 2014-06-13T19:53:36Z : No. of bitstreams: 1 paiva_mb_me_araca.pdf: 916442 bytes, checksum: 4f58624ad7c03912e0dcd44c4e383d66 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O objetivo dessa pesquisa foi analisar a resposta imune celular de cães negativos (GI), vacinados (GII) e positivos (GIII) para a LVC por meio da imunocitoquímica para os anticorpos anti-CD4, anti-CD8 e FOXP3 na capa leucocitária e medula óssea. Na capa leucocitária, quando comparados os grupos entre si, não houve diferença estatística para as células T CD4. Os grupos I e III apresentaram maior imunomarcação para anti-CD8. Embora não tenha sido observada diferença estatística (p>0,05), a imunomarcação para FOXP3 mostrou os menores valores percentuais nos três grupos estudados. Os grupos I e II apresentaram maior imunomarcação para anti-CD4, seguida do anti-CD8, quando comparados os anticorpos em cada grupo separadamente. Nos animais do grupo III, foi observada maior expressão de células imunorreativas ao anti-CD8 seguida do anti-CD4, e valores reduzidos para FOXP3. Nas células da medula óssea não foi verificada diferença estatística na expressão dos anticorpos anti-CD4, anti-CD8 e FOXP3 quando comparados os grupos entre si. Na análise comparativa entre os anticorpos testados, entretanto, foi observada maior marcação para o anticorpo anti-CD4 em GI, ausência de diferença estatística em GII e maior percentual de células positivas para anti-CD8 em GIII. Os valores de FOXP3 também se mantiveram reduzidos em todos os grupos. A técnica de cito inclusão foi padronizada com sucesso, obtendo 68% de viabilidade para amostras da capa leucocitária e medula óssea. Os resultados obtidos neste estudo indicam que a presença de células T CD4+ e CD8+ em animais vacinados, em associação à expressão reduzida do marcador de células T regulatórias FOXP3, sugerem que a vacina Leishmune® exerce um efeito estimulatório na resposta imune celular de cães, promovendo um incremento da resposta protetora Th1 / The aim of this research was to analyze the cellular immune response of negative (GI), vaccinated (GII) and positive (GIII) dogs for VCL by immunocytochemistry for CD4, CD8 and FOXP3 antibodies on the buffy coat and bone marrow. In the buffy coat, when compared the three groups together, there was no statistical difference in CD4+ T cells. Groups I and III showed higher immunostainning for anti-CD8. Although no statistical difference was observed, immunostainning for FOXP3 showed the lowest percentages in all three groups. Groups I and II showed higher immunoreactivity to anti-CD4 followed by anti-CD8 antibodies when compared each group separately. In group III, we observed increased expression of immunoreactive cells to anti- CD8 followed by anti-CD4, and reduced values for FOXP3. In bone marrow cells there was no statistical difference in the expression of anti-CD4, anti-CD8 and FOXP3 antibodies in the three groups. The comparative analysis between the antibodies tested, however, revealed greater cellularity expressing anti-CD4 in GII, and higher percentage of anti-CD8 positive cells in GIII. The group II showed no statistical difference between the antibodies analyzed. FOXP3 values also remained low in all groups. The results of this study indicate that the presence of CD4 + and CD8 + T lymphocytes in the vaccinated animals, combined with reduced expression of the marker of regulatory T cells FOXP3 suggest that Leishmune ® vaccine exerts a stimulatory effect on immune response of dogs, promoting an increase in the protective Th1 response
250

Estudo comparativo entre as técnicas de video-toracoscopia e toracotomia para fenestração de disco intervertebral em cães

Almeida, Tiago Ladeiro de [UNESP] 01 June 2007 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:31:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-06-01Bitstream added on 2014-06-13T20:41:22Z : No. of bitstreams: 1 almeida_tl_dr_jabo.pdf: 626108 bytes, checksum: a2ec25d6198c9a4a04ead0e19b1e3d0b (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A estabilidade da coluna depende de estruturas ósseas e tecidos moles, e traumatismos ou degenerações destes levam a diversas enfermidades medulares. Os discos intervertebrais, por exemplo, quando degenerados podem causar protrusão ou extrusão de seu material para o canal medular, com subseqüente compressão da medula, principalmente na região tóraco-lombar, sendo o tratamento cirúrgico normalmente indicado. Dentre as técnicas descritas na literatura, a fenestração discal apresenta menor porcentagem de recidivas, porém, é extremamente traumática pela necessidade de toracotomia para acesso aos discos. Foi realizado um estudo comparativo entre este procedimento e o vídeo-assistido (VATS), que é uma cirurgia minimamente invasiva, para fenestração discal entre décima primeira e décima terceira vértebras torácicas (T11-T13), em 12 cães (seis por técnica). Após a cirurgia os animais foram submetidos a exames clínicos, neurológicos e radiográficos. Além desta comparação, colunas de 13 cadáveres (cinco operados por acesso padrão, cinco por VATS e três controle), foram avaliadas para comparação das estruturas envolvidas e material discal remanescente. Concluiu-se com base nos resultados obtidos que o procedimento de fenestração de discos intervertebrais por VATS proporciona resultados similares ao tradicional, em segurança e eficácia, porém apresenta vantagens no estágio de convalescença do animal. / Spinal stability is dependant of bone and tissue structures, and traumatisms or degenerations of these can lead to many spinal cord enfermities. Intervertebral discs, for example, when degenerated, can lead to protrusion or extrusion of their material to the medular space, with subsequent compression of this, specialy in the thoraco-lumbar region, being the surgical treatment often indicated. Among the literature described techniques, the discal fenestration has lower porcentual of recurrence, but is extremely traumatic by the necessity of a thoracotomy to access the discs. Comparative study was realized between this procedure and a video-assisted one (VATS), wich is a minimally invasive surgery, being realized discal fenestration of discs between eleventh and thirteenth vertebras (T11-T13), in 12 dogs (six each one). After surgery, these animals were submited to clinical, neurological and radiographic evaluations. Besides this comparation, spines of 13 dog corpses (five operated by tradicional technique, five by VATS and three controls) were avaliated to compare involved structures and remanescent disc material. By these data we can conclude that the disc fenestration by VATS has similar results to the tradicional procedure, in safety and eficacy, with some advantages in the animal's convalescence stage.

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