Submitted by PPG Gerontologia Biom?dica (geronbio@pucrs.br) on 2017-08-18T13:53:17Z No. of bitstreams: 1 GOMES_SONIA_BEARIZ_DA_SILVA_TESE.pdf: 2053994 bytes, checksum: c7c7fbed6de25882df49a1d81fc9d5c2 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-08-21T12:59:52Z (GMT) No. of bitstreams: 1 GOMES_SONIA_BEARIZ_DA_SILVA_TESE.pdf: 2053994 bytes, checksum: c7c7fbed6de25882df49a1d81fc9d5c2 (MD5) / Made available in DSpace on 2017-08-21T13:05:29Z (GMT). No. of bitstreams: 1 GOMES_SONIA_BEARIZ_DA_SILVA_TESE.pdf: 2053994 bytes, checksum: c7c7fbed6de25882df49a1d81fc9d5c2 (MD5) Previous issue date: 2017-05-26 / The 21st century reserves a change in the world population profile, we will have more people over 60 years of age, the proportion of the population "older", that is, more than 80 years of the Brazilian population, is also increasing. This "older" contingent needs, daily, care and assistance to carry out the activities of daily living - ADLs. Part of this long-lived population are consecrated religious who dedicated their lives to God from a religious congregation and thus depend on the structure of the congregation to live this period of the Life Cycle described as finitude. The general objective of this research was to understand the social construction of finitude of life through the meanings of the world lived and not lived by the Marist Brothers in the perspective of understanding the finite phase of the life cycle, starting from the construction, a posteriori, of emerging categories of the Process articulated with the great themes of the formation of consecrated life: Communion with Nature, Communion with others, Communion with oneself and Communion with God. The approach of this research is qualitative, and it follows the ethnographic technique, based on the records systematized from the field journals, ethnographic interview, photos and filming performed from September 2012 to December 2013, of the Project developed by the Pro-Rectory of Extension - PROEX / PUCRS, for the residents of Casa S?o Jos? de Viam?o. The research took as reference the method of analysis of Husserl's Transcendental Phenomenology, which may be aimed at understanding the process of social construction of the world lived and not lived by the Marist Brothers, characterizing it as an interdisciplinary project. The study included 13 Marist Brothers, aged between 76 and 94, and 50 and 74 years of Consecrated Life. The analysis of ethnographic records indicated that the biological, psychological and social aspects of this period of life described as finitude of the participants portray the meanings of life constructed from Marist values, and are overcome in particular by the constitution and preservation of Faith lived , Permeated by the vicissitudes of the aging process. / O s?culo XXI reserva uma mudan?a no perfil populacional mundial, teremos cada vez mais pessoas com idade superior aos 60 anos de idade, as propor??es da popula??o ?mais idosa?, ou seja, de mais de 80 anos da popula??o brasileira, tamb?m est? aumentando. Este contingente ?mais idoso? necessita, diariamente, de cuidados e aux?lio para realizarem as atividades de vida di?ria ? AVDs. Fazem parte desta popula??o longeva, religiosos consagrados que dedicaram sua vida a Deus a partir de uma congrega??o religiosa e, assim, dependem da estrutura da congrega??o para viver este per?odo do Ciclo Vital, descrito como finitude. O objetivo geral desta investiga??o foi compreender a constru??o social de finitude de vida atrav?s dos significados do mundo vivido e n?o vivido pelos Irm?os Maristas na perspectiva do entendimento da fase de finitude do ciclo vital, a partir da constru??o, a posteriori, de categorias emergentes do processo articuladas com os grandes temas da forma??o da Vida Consagrada: Comunh?o com a Natureza, Comunh?o com os outros, Comunh?o consigo mesmo e Comunh?o com Deus. A abordagem desta investiga??o ? qualitativa, e segue a t?cnica etnogr?fica, baseada nos registros sistematizados a partir dos di?rios de campo, entrevista etnogr?fica, fotos e filmagens realizados no per?odo de setembro de 2012 a dezembro de 2013, do Projeto desenvolvido pela Pr?-Reitoria de Extens?o ? PROEX/PUCRS, para os moradores da Casa S?o Jos? de Viam?o. A pesquisa tomou como refer?ncia o m?todo de an?lise a Fenomenologia Transcendental de Husserl, que pode estar voltada para a compreens?o do processo de constru??o social do mundo vivido e n?o vivido pelos Irm?os Maristas, caracterizando-o como um projeto interdisciplinar. Participaram do estudo 13 Irm?os Maristas, com idade entre 76 e 94 anos, e 50 e 74 anos de Vida consagrada. A an?lise dos registros etnogr?ficos indicou que os aspectos biol?gicos, psicol?gicos e sociais deste per?odo de vida descrito como finitude dos participantes, retrata os significados da vida constru?da a partir dos valores Maristas, e s?o superados, em especial, pela constitui??o e preserva??o da F? vivida, permeado pelas vicissitudes do processo de envelhecimento.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-08-25T18:56:36Z No. of bitstreams: 1 TES_DANIEL_DOLIVEIRA_SILVA_CONFIDENCIAL.pdf: 262553 bytes, checksum: 4a53c6ec3ea6da2ddc6cd64d93f5925c (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-08-25T18:56:47Z (GMT) No. of bitstreams: 1 TES_DANIEL_DOLIVEIRA_SILVA_CONFIDENCIAL.pdf: 262553 bytes, checksum: 4a53c6ec3ea6da2ddc6cd64d93f5925c (MD5) / Made available in DSpace on 2017-08-25T18:56:55Z (GMT). No. of bitstreams: 1 TES_DANIEL_DOLIVEIRA_SILVA_CONFIDENCIAL.pdf: 262553 bytes, checksum: 4a53c6ec3ea6da2ddc6cd64d93f5925c (MD5) Previous issue date: 2017-05-19 / Purpose: to determine the usefulness of serum TF as a potential marker for patients with clear cell RCC. Materials and Methods: prospective study of 30 patients with clear cell RCC submitted to nephrectomy and 16 controls without clear cell RCC treated surgically for other conditions. TF is a endothelium marker that was correlated with worse prognosis in a variety of solid tumors including RCC. Serum TF was collected before surgery at the operating room and in the postoperative setting after at least four weeks. Serum samples were analyzed with a commercial ELISA kit for human TF (R&D Systems ?). Results: Mean preoperative serum TF levels in clear cell RCC patients and in controls were 66.8 pg/dl and 28.4 pg/dl, respectively (p<0.001). Mean postoperative serum TF levels in clear cell RCC patients were 26.3 pg/dl. In all patients with clear cell RCC postoperative serum levels of TF were lower, with a mean reduction of 41.6 pg/dl in the postoperative setting (p<0.001). Linear regression revealed that tumor size was correlated with the postoperative reduction of serum TF levels (p=0.037). Conclusions: We have shown a 3-fold reduction in the median preoperative serum levels of TF in patients with clear cell RCC after surgery. We have also shown a difference of the same magnitude in the serum levels of TF compared with those of a control group of patients with benign diseases. TF appears to be a useful serum marker for the presence of clear cell RCC. Further studies are needed to validate these findings. / O c?ncer renal ? uma das neoplasias malignas mais letais. As taxas de mortalidade t?m crescido mundialmente a despeito da evolu??o do diagn?stico precoce e do surgimento de novos f?rmacos ativos na terapia da doen?a sist?mica. Atualmente, para se estabelecer riscos de morbimortalidade em c?ncer renal os pacientes s?o classificados de acordo com escalas e nomogramas, que ainda n?o conseguem predizer com grande acur?cia a evolu??o cl?nica dos mesmos. Nosso estudo foi realizado com o objetivo de determinar a utilidade da dosagem do Fator Tecidual (FT) no soro como potencial marcador para pacientes comCarcinoma de C?lulas Claras de Rim (CCCr).Realizamos um estudo prospectivo com 30 pacientes com CCCr submetidos ? nefrectomia e 16 controles sem CCCr tratados cirurgicamente para outras condi??es. O FT s?rico foi coletado na sala cir?rgica antes do procedimento e no p?s-operat?rio ap?s pelo menos quatro semanas. Os n?veis s?ricos detectados no pr?-operat?rio foram tr?s vezes mais altos em pacientes com CCCr do que nos controles, e dininu?ram em todos os pacientes com CCCr no p?s-operat?rio, com uma redu??o m?dia de 41,6 pg/ml (p <0,001). Nossos achados sugerem que oFTpode ser utilizado como um marcador s?rico para a presen?a de CCCr.

Submitted by PPG Pediatria e Sa?de da Crian?a (pediatria-pg@pucrs.br) on 2017-10-09T18:06:32Z No. of bitstreams: 1 DISSERTA??O_KANAMA_04102017.pdf: 951565 bytes, checksum: 5231156d8d70ba29deeb6acef8d2d3fc (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-10-10T13:27:13Z (GMT) No. of bitstreams: 1 DISSERTA??O_KANAMA_04102017.pdf: 951565 bytes, checksum: 5231156d8d70ba29deeb6acef8d2d3fc (MD5) / Made available in DSpace on 2017-10-10T13:28:41Z (GMT). No. of bitstreams: 1 DISSERTA??O_KANAMA_04102017.pdf: 951565 bytes, checksum: 5231156d8d70ba29deeb6acef8d2d3fc (MD5) Previous issue date: 2017-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Introduction: acute bronchiolitis is one of the most common respiratory diseases of early childhood. It is the main cause for hospitalization of children in developed countries during winter time. Currently, despite advances in medicine, there is still no evidence of specific and effective treatment for acute bronchiolitis. In order to prevent the disease, the Brazilian Ministry of Health, in July 2013, has made palivizumab available to high-risk children throughout Brazil. Objective: to assess the trend of hospitalization for acute bronchiolitis in infants under 1 year of age, before and after the implementation of the palivizumab immunization program in Brazil. Methods: retrospective analysis of data from infants under 1 year of age hospitalized with a diagnosis of acute bronchiolitis between 2008 and 2015 in Brazil, using a database of the Brazilian National Health System. The incidence of pre-implementation (2008-2012) and post-implementation (2014-2015) admissions was analyzed. Results: between January 2008 and December 2015, 263,654 hospitalizations for bronchiolitis were registered in infants under 1 year of age, 60% represented by boys. The incidence of hospitalization for bronchiolitis increased 49,4% during this period (from 8.5 to 12.7 per 1000 inhabitants per year), with an annual incidence rate of 10.9 per 1000 inhabitants per year. Between 2013 and 2014, the incidence rate of hospitalization for acute bronchiolitis decreased 8% (from 12.5 to 11.5 per 1000 inhabitants per year). In the second year of the program, the hospitalization rate increased 10% again (from 11.5 to 12.7 per 1000 inhabitants? year). Conclusion: the incidence of acute bronchiolitis showed an increasing trend. Hospitalizations declined one year after implementation of palivizumab and returned to increasing trend in the second year of the program. / Introdu??o: a bronquiolite aguda ? uma das doen?as respirat?rias mais comuns da primeira inf?ncia e constitui o principal motivo de interna??o de crian?as em unidades de emerg?ncia e interna??o pedi?tricas em pa?ses desenvolvidos, no per?odo de inverno. Atualmente, apesar dos avan?os na medicina, ainda n?o existe evid?ncia de tratamento especifico eficaz para bronquiolite aguda. Visando prevenir a doen?a, desde julho de 2013, o Minist?rio da Sa?de do Brasil disponibilizou a imuniza??o com palivizumabe para crian?as de alto risco em todo o territ?rio brasileiro. Objetivo: avaliar a tend?ncia de hospitaliza??o por bronquiolite aguda em lactentes menores de 1 ano de idade, antes e ap?s a implementa??o do programa de imuniza??o por palivizumabe no Brasil. M?todos: an?lise retrospectiva dos dados de lactentes menores de 1 ano de idade, hospitalizados com diagn?stico de bronquiolite aguda entre 2008 e 2015 no Brasil, utilizando o banco de dados do Sistema ?nico de Sa?de (DATASUS). Foram avaliadas as taxas de hospitaliza??o nos per?odos pr?- (2008-2012) e p?s-implementa??o (2014-2015) do programa de imuniza??o por palivizumabe. Resultados: entre janeiro de 2008 a dezembro 2015 foram registradas 263.654 interna??es por bronquiolite aguda em lactentes menores de 1 ano de idade, 60% representado por meninos. A incid?ncia de hospitaliza??o por bronquiolite aumentou 49,4% ao longo desse per?odo (de 8,5 para 12,7 por 1000 habitantes por ano), com taxa de incid?ncia m?dia anual de 10,9 por 1000 hab/ano. Entre 2013 e 2014, a taxa de incid?ncia de hospitaliza??o por BA diminuiu 8% (de 12,5 para 11,5 por 1000 habitantes por ano). Por?m, no segundo ano da implementa??o do programa, a taxa de interna??o aumentou novamente 10% (para 12,7 por 1000 habitantes por ano). Conclus?o: a bronquiolite aguda apresentou taxas de hospitaliza??o crescente ao longo do per?odo estudado. A incid?ncia de hospitaliza??es apresentou um decl?nio um ano ap?s implementa??o de palivizumabe e retornou a tend?ncia crescente no segundo ano do programa.

Submitted by PPG Biotecnologia Farmac?utica (mpbf@pucrs.br) on 2017-10-05T18:16:51Z No. of bitstreams: 1 DAIANE_BRIDI-DIS.pdf: 2089124 bytes, checksum: c80421954ce799cf7f5b1206f22ec59a (MD5) / Rejected by Caroline Xavier (caroline.xavier@pucrs.br), reason: Devolvido devido ? falta de capa institucional no arquivo PDF, e a publica??o est? cadastrada como "tese" on 2017-10-06T13:39:56Z (GMT) / Submitted by PPG Biotecnologia Farmac?utica (mpbf@pucrs.br) on 2017-10-18T11:48:05Z No. of bitstreams: 1 DAIANE_BRIDI-DIS.pdf: 2205656 bytes, checksum: 0d6301199a5443bf6ca3d070c2d27d6e (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-10-20T12:39:52Z (GMT) No. of bitstreams: 1 DAIANE_BRIDI-DIS.pdf: 2205656 bytes, checksum: 0d6301199a5443bf6ca3d070c2d27d6e (MD5) / Made available in DSpace on 2017-10-20T12:43:25Z (GMT). No. of bitstreams: 1 DAIANE_BRIDI-DIS.pdf: 2205656 bytes, checksum: 0d6301199a5443bf6ca3d070c2d27d6e (MD5) Previous issue date: 2017-08-11 / Glyphosate has become the most widely used herbicide in the world, due to the wide scale adoption of resistant crops, after its introduction in 1996. Glyphosate can be used alone, but is commonly used as an active ingredient of the Roundup? herbicide. This herbicide contains several adjuvants in addition to glyphosate, which may promote an unknown e.g. toxicity Polioxietilenamida (POEA). Zebrafish is gaining popularity in behavioral research, because of its physiological similarity to mammals, ease of manipulation, robust performance, low cost, external fertilization, transparency of embryos larval stages and rapid development. The aim of this study was to evaluate the effects of glyphosate and Roundup? on behavioral and morphological parameters in zebrafish larvae and adult. Zebrafish larvae at 3 days post-fertilization (dpf) and adults were exposed to glyphosate (0.01, 0.065, and 0.5 mg/L) and Roundup? (0.01, 0.065, and 0.5 mg/L) for 96 hours. Immediately after the treatment, behavioral parameters such as locomotor activity and aversive behavior and morphology for the larvae and locomotion, agressive behavior and memory for the adults were analyzed. Zebrafish larvae, the results indicated that there were significant differences in the locomotor activity and aversive behavior by glyphosate and Roundup? when compared to the control. However, there was a decrease in distance traveled and the time spent in zone without stimulation for exposed larvae at doses of glyphosate and Roundup?. A significant decrease in body lenght was observed for larvae exposed to Roundup? in all concentrations tested. Our findings demonstrated that glyphosate and Roundup? exposure reduced the distance traveled, the mean speed and the line crossings in the highest concentration of glyphosate (0.5 mg / L) and 0.065 and 0.5mg/L Roundup? in animals adults. We verified that Roundup?-treated adult zebrafish showed a significant impairment in memory. Our results showed that glyphosate and Roundup? had an effect on agressive behavior. Our findings demonstrated that the effects of isolated and commercial forms of glyphosate promoted differences on locomotion, behavior and morphology of the treated animal, suggesting similar mechanisms of toxicity and cellular response. / O glifosato tornou-se o herbicida mais utilizado no mundo, devido ? ado??o ampla de culturas resistentes, ap?s sua introdu??o em 1996. O glifosato pode ser usado sozinho, mas ? comumente utilizado como ingrediente ativo do herbicida Roundup?. Este herbicida cont?m v?rios adjuvantes, tal como a Polioxietilenamida (POEA), que podem promover uma toxicidade desconhecida. O peixe-zebra est? ganhando popularidade na pesquisa comportamental, devido ? similaridade fisiol?gica com os mam?feros, facilidade de manipula??o, baixo custo, fertiliza??o externa, transpar?ncia de embri?es nos est?gios larvais e desenvolvimento r?pido. O objetivo deste estudo foi avaliar os efeitos do glifosato e do Roundup? sobre par?metros comportamentais e morfol?gicos em peixe-zebra no est?gio larval e adulto. As larvas com 3 dias p?s-fertiliza??o (dpf) e adultos foram expostos ao glifosato (0,01, 0,065 e 0,5 mg/L) e Roundup? (0,01, 0,065 e 0,5 mg/L) por 96 horas. Imediatamente ap?s o tratamento, realizamos a an?lise de par?metros comportamentais, como atividade locomotora, comportamento aversivo e morfologia para larvas e locomo??o, comportamento agressivo e mem?ria aversiva para adultos. Nas larvas houveram diferen?as significativas na atividade locomotora e comportamento aversivo nos animais tratados com glifosato e Roundup? quando comparado ao controle. Foi observada uma diminui??o na dist?ncia percorrida e na resposta aversiva nas larvas expostas ao glifosato e Roundup?. Observou-se uma diminui??o significativa no comprimento corporal das larvas expostas ao Roundup? em todas as concentra??es testadas. Nossos resultados demonstraram que a exposi??o ao glifosato ou Roundup? reduziu a dist?ncia percorrida, a velocidade m?dia e o n?mero de cruzamentos na maior concentra??o de glifosato (0,5mg/L) e 0,065 e 0,5mg/L de Roundup? em animais adultos. Verificamos que peixe-zebra adulto tratado com Roundup? apresentou um comprometimento significativo na mem?ria. Nossos resultados demostraram que o glifosato e o Roundup? tiveram efeito sobre o comportamento agressivo. Assim, nossos achados demonstraram que os efeitos das formas isoladas e comerciais de glifosato promoveram diferen?as na locomo??o, comportamento e morfologia do animal tratado, sugerindo mecanismos semelhantes de toxicidade e resposta celular.

Submitted by PPG Pediatria e Sa?de da Crian?a (pediatria-pg@pucrs.br) on 2018-01-02T10:33:31Z No. of bitstreams: 1 Tese Doutorado Felipe Kalil Final.pdf: 7524023 bytes, checksum: 981fc086520772a8909e9bd61eeb31df (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-01-02T16:33:29Z (GMT) No. of bitstreams: 1 Tese Doutorado Felipe Kalil Final.pdf: 7524023 bytes, checksum: 981fc086520772a8909e9bd61eeb31df (MD5) / Made available in DSpace on 2018-01-02T16:42:48Z (GMT). No. of bitstreams: 1 Tese Doutorado Felipe Kalil Final.pdf: 7524023 bytes, checksum: 981fc086520772a8909e9bd61eeb31df (MD5) Previous issue date: 2016-08-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Objective/Background: Either epilepsy or attention deficit hyperactivity disorder (ADHD) can influence in different ways sleep organization. The aim of this study is to evaluate quality of sleep in children and adolescents with ADHD and epilepsy and to analyze the influence of methylphenidate on sleep organization. Methods: This is an observational, cross sectional study, with children and adolescents with epilepsy, seizure free for at least 3 months, and with ADHD, selected from the epilepsy and child neurology outpatient clinic of a tertiary hospital in Brazil. Patients were divided into four different groups with 21 patients each: ADHD as comorbidity of epilepsy using methylphenidate; ADHD as comorbidity of epilepsy not using methylphenidate; only ADHD and a health control group. All participants were evaluated with the Sleep Disturbance Scale for Children and monitored with actigraphy for five nights. Results: Actigraphic analysis showed a higher number of night awakenings at the epilepsy/ADHD groups, most prominent at the group without methylphenidate (p=0,001). Parental reports demonstrated a higher risk for sleep disturbances at the epilepsy/ADHD without methylphenidate and ADHD groups (p<0,001). Conclusion: Primary ADHD or ADHD as a comorbidity of epilepsy impairs sleep organization in children. The use of methylphenidate seems to improve sleep quality. / Objetivo: Sabe-se que tanto a epilepsia quanto o Transtorno do d?ficit de aten??o e hiperatividade (TDAH) podem influenciar a organiza??o do sono de diferentes maneiras. O objetivo deste estudo ? avaliar a qualidade do sono em crian?as e adolescentes com TDAH e epilepsia e analisar a influ?ncia do metilfenidato sobre a organiza??o do sono. M?todos: Estudo transversal, observacional, em crian?as e adolescentes com epilepsia, sem crises por pelo menos 3 meses, e com TDAH, selecionados dos ambulat?rios de epilepsia e neurologia infantil de um hospital terci?rio no Brasil. Os pacientes foram divididos em 4 diferentes grupos, cada um com 21 indiv?duos: TDAH como comorbidade de epilepsia em uso de metilfenidato; TDAH como comorbidade de epilepsia sem uso do metilfenidato; TDAH e grupo controle. Todos os participantes foram avaliados pela Escala de Dist?rbios de Sono em Crian?as e monitorados por actigrafia durante 5 noites. Resultados: A an?lise da actigrafia demonstrou maior n?mero de despertares noturnos para os grupos epilepsia/TDAH, mais proeminentes no grupo sem metilfenidato (p = 0,001). Os relatos dos pais/cuidadores evidenciaram um maior risco de dist?rbios do sono nos grupos epilepsia/TDAH sem metilfenidato e TDAH (p <0,001). Conclus?o: O TDAH prim?rio ou TDAH como comorbidade de epilepsia prejudicam a organiza??o do sono em crian?as. O uso de metilfenidato parece melhorar a qualidade do sono.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-11-03T11:11:53Z No. of bitstreams: 1 TES_DENIS_SOUTO_VALENTE_PARCIAL.pdf: 425112 bytes, checksum: da5a1914e0af743bea4b0e364bdd898a (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-11-03T11:12:35Z (GMT) No. of bitstreams: 1 TES_DENIS_SOUTO_VALENTE_PARCIAL.pdf: 425112 bytes, checksum: da5a1914e0af743bea4b0e364bdd898a (MD5) / Made available in DSpace on 2017-11-03T11:12:48Z (GMT). No. of bitstreams: 1 TES_DENIS_SOUTO_VALENTE_PARCIAL.pdf: 425112 bytes, checksum: da5a1914e0af743bea4b0e364bdd898a (MD5) Previous issue date: 2017-05-25 / Background- Perception of facial age is a health biomarker. Bariatric surgery provides benefits to obese people. Knowledge is scarce about the role of obesity in facial age. Objectives- The aim of this study is to verify whether there is a change in the perception of facial age when comparing people with morbid obesity before surgery and one year after the procedure, along with normal weight controls. Methods- Through a prospective cohort, subjects with morbid obesity were reassessed after undergoing bariatric surgery. In a transversal study, these individuals were compared to people of normal weight. Seven plastic surgeons estimated the age of each subject based solely on their photographs. The difference in each age perceived was calculated. Results- Before surgery the mean facial age perception was 40.8 years and after was 43.7 years (p = 0.004). In the control group a mean difference of -1.03 years was observed, different from morbid obesity (p = 0.045) and after bariatric surgery (p = 0.001). Men over 40 years of age, with a preoperative body mass index between 40 kg and 49.9 kg/m2, weight greater than 127.65 kg before surgery, and the percentage loss of excess weight greater than 75.13%, demonstrated greater perceived facial aging. Conclusion- Bariatric surgery produces facial aging. Morbidly obese people and individuals who have undergone bariatric surgery appear to be older than people who have always been thin. / Introdu??o- A percep??o da idade facial ? um biomarcador de sa?de. A cirurgia bari?trica proporciona benef?cios para pessoas obesas. O conhecimento ? escasso acerca do papel da obesidade na idade facial. Objetivos- Verificar se existe modifica??o na percep??o da idade facial ao comparar pessoas com obesidade m?rbida antes da cirurgia e um ano ap?s a realiza??o desta, bem como controles de peso normal. M?todos- Atrav?s de uma coorte prospectiva os sujeitos com obesidade m?rbida foram reavaliados ap?s passarem pela cirurgia bari?trica. No estudo transversal estes mesmos indiv?duos foram comparados com pessoas de peso normal. Sete cirurgi?es pl?sticos presumiram a idade de cada sujeito somente atrav?s de suas fotografias. Foi calculada a diferen?a de cada idade percebida. Resultados- Antes da cirurgia a m?dia de percep??o da idade facial foi de 40,8 anos e ap?s foi de 43,7 anos (p=0,004). No Grupo controle se observou a diferen?a na percep??o da idade facial de -1,03 anos sendo diferente da obesidade m?rbida (p= 0,045) e ap?s a cirurgia (p=0,001). Homens, maiores de 40 anos, ?ndice de massa corporal pr?-operat?rio entre 40 e 49,9 kg/m2, peso maior do que 127,65 kg antes da cirurgia, perda de peso menor do que 43,15 kg e perda do percentual de excesso de peso maior do que 75,13% apresentaram maior envelhecimento facial. Conclus?o- A cirurgia bari?trica produz envelhecimento facial. Pessoas com obesidade m?rbida e indiv?duos que se submeteram a cirurgia bari?trica aparentam ter mais idade do que pessoas que sempre foram magras.

Submitted by PPG Biotecnologia Farmac?utica (mpbf@pucrs.br) on 2017-12-20T19:17:22Z No. of bitstreams: 1 ANA_SOFIA_OLIVEIRA-DIS.pdf: 959707 bytes, checksum: 29ee77d841ca692f44c6db51a03d5a23 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-12-26T17:46:20Z (GMT) No. of bitstreams: 1 ANA_SOFIA_OLIVEIRA-DIS.pdf: 959707 bytes, checksum: 29ee77d841ca692f44c6db51a03d5a23 (MD5) / Made available in DSpace on 2017-12-26T17:48:46Z (GMT). No. of bitstreams: 1 ANA_SOFIA_OLIVEIRA-DIS.pdf: 959707 bytes, checksum: 29ee77d841ca692f44c6db51a03d5a23 (MD5) Previous issue date: 2017-06-20 / Dengue fever is an arboviral infection highly common in Brazil, and it corresponds to a major public health problem. Annually, its incidence overcomes 50,000,000, from which about 25,000 are associated to death cases. Due to this high incidence and number of death, it is of great urgency to identify this disease while in an early state. That way, would be possible to avoid any progression of the disease to more severe cases. A major problem related to dengue is that the virus is represented by four subtypes, which are phylogenetically distinct (DENV1, DENV2, DENV3 and DENV4). This means that, although the manifestations and the forms of dissemination are the same, an infection caused by one of the serotypes will not protect against the others. In fact, an infection caused by one of the four serotypes may intensify even more the disease caused by a secondary infection by the three others. One hypothesis that could explain this relationship between heterotypic infections and severe cases of the disease is the ADE hypothesis (antibody-dependent enhancement), which suggests that a secondary infection would cause a cross-reaction between antigen and antibody, preventing the virus of being inactivated. As a consequence, it would result in an increase of the production of inflammatory mediators and vascular permeability that would intensify the disease. Therefore, it?s very important to identify the disease while it is still in an initial stage. However, this early identification is hard and its diagnosis is still limited. Thus, a serological marker would be highly valuable. Recently, serological biomarkers for early diagnosis have become a topic of great interest. These biomarkers have advantages of not being invasive to the patient and inexpensive to produce and analyze. Thus, in this project, we introduce a new technology for the search of such markers, the peptoids. Peptoids are synthetic oligomers, composed of N-substituted glycine units, and can be used for several biological utilities that provide an alternative technology for the investigation and the elucidation of the immune response. Moreover, they have been used and reported as a potential candidate for the search of serological biomarkers due to their chemical stability in fluids where degradative enzymes can be found. In addition to that, the fact that there is no need for a prior knowledge about the target only make them even more attractive for such ?job?. In this project, a combinatorial library of about one million of peptoids was screened in sera from dengue positive patients, as well as negative patients. Hereby we report the potential of this synthetic molecules for the identification of antibodies with clinical relevance present in the patients' serum. The results here reported were generated by 3 consecutive screening steps, where 33 reactive peptoids were identified as potential biomarkers. Because of the impartiality of this technique, we believe that at least one of these 33 peptoids are extremely specific for the studied disease. Currently, they have been sent for the sequencing and re-synthesis step, so that new experiments can be done and the study can continue. We are very excited and we don?t expect nothing less than promising results in the field of diagnosis and dengue. / A dengue ? uma arbovirose de alta incid?ncia no Brasil, e corresponde a um grande problema de sa?de p?blica. Ela possui uma incid?ncia anual de mais de 50.000.000 e resulta num elevado n?mero de casos de ?bito, cerca de 25.000. Devido a isso, ? de grande urg?ncia que possamos identificar esta doen?a enquanto ela ainda se encontra em um estado inicial, para que, deste modo, possamos evitar a progress?o da doen?a para casos mais severos. Um grande problema relacionado a dengue ? que o v?rus ? representado por quatro subtipos, os quais s?o filogen?ticamente distintos (DENV1, DENV2, DENV3 e DENV4). Isso quer dizer que, por mais que as manifesta??es e formas de dissemina??o sejam iguais, a contamina??o por um dos sorotipos n?o ir? proteger contra as demais. Ao contr?rio do que muitos acreditam, a infec??o por um sorotipo pode at? assentuar uma infec??o secund?ria pelos demais. Uma hip?tese para explicar casos mais severos da doen?a devido a infec??es heterot?picas ? a hip?tese de ADE (antibody-dependent enhancement), a qual sugere que a rea??o cruzada resulta na falta da inativa??o viral, aumentando assim a produ??o de mediadores inflamat?rios e permeabilidade vascular devido a uma estimula??o da replica??o do v?rus. Devido a isso, ? de grande necessidade a identifica??o da doen?a antes das manifesta??es severas, e, porque essa identifica??o cl?nica, principalmente no est?gio inicial da doen?a, ? dif?cil, um marcardor sorol?gico seria muito valioso. Marcadores sorol?gicos para diagn?stico de doen?as v?m se tornando um t?pico de recente interesse. Esses biomarcadores t?m a vantagem de n?o serem invasivos para o paciente e baratos de produzir e analizar. Sendo assim, aqui, apresentamos uma nova tecnologia para a busca desses marcadores, os pept?ids. Pept?ids s?o olig?meros sint?ticos, compostos por unidades de glicina N-substitu?da e possuem uma variada utilidade biol?gica que proporcionam uma tecnologia alternativa para a investiga??o e elucida??o da resposta imune. O mesmo, v?m se destacando para busca de biomarcadores sorol?gicos devido a sua estabilidade qu?mica em flu?dos onde enzimas degradativas podem estar presentes e devido a resultados promissores sem a necessidade de um conhecimento pr?vio sobre o ant?geno natural e o seu anticorpo correspondente. Neste projeto, foi realizado a triagem de uma biblioteca combinatorial com cerca de um milh?o de pept?ids, os quais passaram por etapas de triagens em soros de pacientes positivos para dengue, bem como para pacientes negativos. Aqui provamos o potencial dessas mol?culas sint?ticas na identifica??o de anticorpos com relev?ncia cl?nica presente no soro dos pacientes. Os resultados obtidos foram gerados atrav?s de 3 etapas de triagem consecutivas, onde 33 pept?ids reativos foram identificados. Devido a imparcialidade desta t?cnica, acreditamos que pelo menos um desses 33 pept?ids seja extremamente espec?fico para a doen?a estudada. Os mesmos foram enviados para sequenciamento e re-s?ntese para que novos experimentos sejam feitos e para que os estudos continuem. Espera-se resultados muito promissores na ?rea de diagn?stico para dengue.

Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2017-12-26T13:35:05Z No. of bitstreams: 1 GIOVANNA_MEDEIROS_TAVARES_DE_OLIVEIRA_TES.pdf: 3690052 bytes, checksum: e6e9b4fea2305637782a214e8f45af2d (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-12-29T10:45:02Z (GMT) No. of bitstreams: 1 GIOVANNA_MEDEIROS_TAVARES_DE_OLIVEIRA_TES.pdf: 3690052 bytes, checksum: e6e9b4fea2305637782a214e8f45af2d (MD5) / Made available in DSpace on 2017-12-29T10:47:48Z (GMT). No. of bitstreams: 1 GIOVANNA_MEDEIROS_TAVARES_DE_OLIVEIRA_TES.pdf: 3690052 bytes, checksum: e6e9b4fea2305637782a214e8f45af2d (MD5) Previous issue date: 2017-08-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Initially used in magnetic resonance imaging in the late 1970s, iron oxide nanoparticles (IONPs) have wide application in the medical field today, in diagnostics, drug delivery, cellular therapies and theragnostic. The high biocompatibility, small size, functionalization and ability to respond to an applied magnetic field gives this nanoparticle great advantage over other nanomaterials. Studies have demonstrated the low toxicity and high applicability of this nanomaterial in the clinic, however some questions remain unanswered. When in contact with the in vivo metabolism, nanomaterials can behave in a way to degrade their coating and release the ions contained in their nucleus. In fact, side effects related to exposure to IONPs are mainly related to the release of its elemental nucleus; which, when endocyted, can be degraded inside the lysosomes and release [Fe] ions. Changes in iron homeostasis can be very damaging to the cell, causing inflammation, lipid peroxidation, and oxidative stress. Organs more sensitive to iron accumulation, such as the heart, may demonstrate apoptosis and tissue degeneration. Such toxic effects are easily detected in studies using zebrafish as an animal model. Protocols with zebrafish embryos for toxicological analysis have the advantage of allowing large-scale screening on development, survival, behavior, gene expression and cardiotoxicity. Under this scenario, this thesis aims to evaluate the toxicity of commertial and "in house" synthesis of Iron Oxide Nanoparticles in zebrafish. Behavioral analysis of locomotion and gene expression of zebrafish larvae exposed to uncoated and dextran-coated iron oxide nanoparticles indicated a toxicity at low concentrations of nanoparticle exposure, contrary to what is reported in the literature. In addition, changes in the apoptotic pathway suggest that this route is closely linked to the behavioral effects found. Subsequent analyzes, targeting cardiotoxicity, suggested that concentrations above 100 ?g/ml are damaging for the heart. Molecular analyzes in the groups exposed to the iron oxide nanoparticle and to iron solution helped to establish a parallel between the toxicity of these nanoparticles and the pathways of iron metabolism. / Inicialmente utilizadas em exames de resson?ncia magn?tica no final dos anos 1970 as nanopart?culas de ?xido de ferro (IONPs) possuem hoje vasta aplica??o na ?rea m?dica, em exames de diagn?stico, sistema de envio de drogas, terapias celulares e como agente teragn?stico. Sua alta biocompatibilidade, pequeno tamanho, facilidade de manipula??o e capacidade de responder a aplica??o externa de campo magn?tico lhe oferece grande vantagem sobre outros nanomaterias. Estudos vem demostrando a baixa toxicidade e alta aplicabilidade deste nanomaterial na cl?nica, entretanto algumas quest?es ainda se encontram sem resposta. Quando em contato com o metabolismo in vivo, nanomateriais podem se comportar de forma a degradar sua estrtutura externa e liberar os ?oins contidos no seu n?cleo. De fato, efeitos adversos relacionado a exposi??o a IONPs est?o majoritariamente relacionadas a libera??o do seu n?cleo elementar; que, quando endocitado, pode ser degradado nos lisossomos e liberar ?ons [Fe]. Altera??es na homeostase de ferro podem ser muito prejudiciais ? c?lula, causando inflama??o, peroxida??o lip?dica e estresse oxidativo. ?rg?os mais sens?veis ao ac?mulo do ferro, como o cora??o, podem apresentar apoptose e degenera??o tecidual. Tais efeitos t?xicos s?o facilmente detectados em estudos utilizando zebrafish como animal modelo. Protocolos com embri?es de zebrafish para an?lise toxicol?gica possuem a vantagem de permitir estudos em grande escala de efeitos no desenvolvimento, sobreviv?ncia, comportamento, express?o g?nica e cardiotoxicidade. Sob esse cen?rio, esta tese tem como objetivo avaliar a toxicidade de Nanopart?culas de Oxido de Ferro (IONPs) de s?ntese pr?pria e comerciais no modelo experimental zebrafish. An?lise comportamental de locomo??o e express?o g?nica de larvas de zebrafish expostas a Nanopart?culas de ?xido de Ferro puras (sem envolt?rio) e revestidas com dextran indicou uma toxicidade em baixas concentra??es de exposi??o ? nanopart?culas, contrario ao que ? relatado na literatura. Al?m disso, altera??es na via apopt?tica sugere que esta rota esteja intimamente ligada aos efeitos comportamentais encontrados. An?lises posteriores, direcionadas ? cardiotoxicidade sugerem efeitos t?xicos acima de 100 ?g/mL. An?lises gen?mica de express?o nos grupos expostos ? nanopart?cula de ?xido de ferro e ? solu??o de ferro met?lico (usado como controle de positivo de excesso de ferro) permitiram a identifica??o de um paralelo entre toxicidade destas nanopart?culas e as vias de metaboliza??o do ferro.

Submitted by PPG Pediatria e Sa?de da Crian?a (pediatria-pg@pucrs.br) on 2018-01-02T17:12:00Z No. of bitstreams: 1 MESTRADO FINAL Dr Sapiro dez2017.pdf: 3077255 bytes, checksum: 57035e21d6aa183e40d7bc92f764603f (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-01-24T12:51:08Z (GMT) No. of bitstreams: 1 MESTRADO FINAL Dr Sapiro dez2017.pdf: 3077255 bytes, checksum: 57035e21d6aa183e40d7bc92f764603f (MD5) / Made available in DSpace on 2018-01-24T12:53:47Z (GMT). No. of bitstreams: 1 MESTRADO FINAL Dr Sapiro dez2017.pdf: 3077255 bytes, checksum: 57035e21d6aa183e40d7bc92f764603f (MD5) Previous issue date: 2017-10-04 / Introduction: There is a limited number of studies that assess students' profile and self-report on the participation of medical students in charitable activities. The most common design is related to extracurricular activities within the technical areas of health. Objective: To describe the profile and self-report of undergraduate medical students on volunteer charitable activities. Methodology: Cross-sectional study; It was a convenience sample with undergraduate medical students (School of Medicine, Pontificia Universidade Cat?lica do Rio Grande do Sul- PUCRS) enrolled from 2014 to 2016. Participants were divided in two groups; one with students that had participated in volunteer charitable activities supported by the university, and another group (control) that had not participated in any of the promoted charitable activities. Both groups answered a self-administered questionnaire, composed of items on sociodemographic data and participation in volunteer activities. We sought to investigate the students' vision regarding the motivation, values, and positive and negative aspects related to such activities. Questions were divided as follows: eight questions to characterize the sample, 16 questions about volunteering, 13 closed questions and 3 open questions. Results: Fifty-nine students were included in the study out of 90 that were invited to participate (66%). There were no significant differences among groups with regards to general characteristics of the participants (sociodemographic variables, course time and family participation in voluntary activities). Students who participated in activities promoted by the institution reported greater collaboration in other volunteer activities. When evaluating the characteristics of students who participated in volunteer activities, the main motivation to participate was personal training 19 (63%); regarding the values most of the students reported more than one. The dimensions that emerged from the testimonies were: Solidarity, Personal formation, Professional training and New experiences. The reports on motivation revealed the care and exchange of experiences with other people, particularly in situations of greater vulnerability, as a tool for personal and professional growth, as well as the opportunity to live new experiences. The reports were not different when one looks for the speech of the academics who did not participate in the activities. In the reports about the positive aspects of participating in activities, the students who participated used sentences with first-person verbs - "I feel better" -, action verbs - "seeing them happy" - and others as almost statements - "It is wonderful meeting new people, listening to your stories "-. The negative points reported did not appear to be related to the activity itself, but to the reasons or justifications for not being able to participate in the activities. The presence of phrases that reported the difficulty of having available time was very present. Conclusion: The present study evidenced that the profile and report of the students who participated in the volunteer actions of the School of Medicine and those who did not participate are similar in several aspects. Both groups consider that participation in voluntary activities is important for personal and professional training / Introdu??o: Existe um n?mero limitado de estudos que avaliam o perfil e os relatos dos alunos sobre as atividades volunt?rias com participa??o de estudantes de medicina relacionadas a car?ter beneficente. O desenho mais comum ? aquele relacionado ?s atividades complementares, extracurriculares, ligadas ?s ?reas t?cnicas da sa?de. Objetivo: Descrever o perfil e os relatos dos acad?micos de gradua??o em medicina sobre as atividades de voluntariado na ?rea social durante Curso da Escola de Medicina da Pontif?cia Universidade Cat?lica do Rio Grande do Sul (PUCRS). Metodologia: O estudo tem delineamento transversal e incluiu alunos de gradua??o da Escola de Medicina da PUCRS, matriculados no per?odo de 2014 a 2016. A inclus?o dos participantes foi feita por conveni?ncia, tendo envolvido um grupo de alunos, casos, que havia participado de pelo menos uma atividade de voluntariado na Escola e de outro grupo que n?o participou de nenhuma destas atividades propostas. O question?rio, auto administrado, foi composto por perguntas sobre dados sociodemogr?ficos e participa??o nas atividades de voluntariado. Buscou-se investigar a vis?o dos alunos em rela??o ? motiva??o, valores, pontos positivos e negativos relacionados ?s atividades. As quest?es foram divididas em oito perguntas para caracteriza??o da amostra e 16 perguntas sobre voluntariado, sendo 13 perguntas fechadas e tr?s abertas. Resultados: Foram convidados a participar da presente pesquisa 90 estudantes. Destes, 59 (66%) aceitaram participar e foram inclu?dos na pesquisa. Ao compararmos os dados das caracter?sticas gerais dos participantes, observamos que os estudantes n?o apresentaram diferen?as significativas em rela??o as vari?veis sociodemogr?ficas, tempo de curso e participa??o familiar em atividades de voluntariado. Os alunos que participaram das atividades relataram uma maior colabora??o em outras atividades de voluntariado. Ao avaliarmos as caracter?sticas apenas dos alunos que participaram das atividades de voluntariado, a principal motiva??o para participar das atividades foi forma??o pessoal, 19 (63%) e em rela??o aos valores 20 (67%) dos alunos relataram mais do que um. As dimens?es que emergiram a partir dos depoimentos foram: Solidariedade, Forma??o pessoal, Forma??o profissional e Novas experi?ncias. Os relatos sobre a motiva??o revelaram o cuidado e troca de experi?ncias com outras pessoas, particularmente em situa??es de maior vulnerabilidade, como uma ferramenta de crescimento pessoal e profissional, assim como oportunidade de vivenciar de novas experi?ncias. Os relatos n?o foram diferentes quando se busca a fala dos acad?micos que n?o participaram das atividades. Nos relatos sobre os pontos positivos de participar nas atividades, os alunos que participaram das a??es usaram mais frases com verbos na primeira pessoa ??me sinto melhor? -, verbos de a??o - ?ver eles felizes?- e outros como quase depoimentos - ?? maravilhoso conhecer pessoas novas, ouvir suas hist?rias? ?. Os pontos negativos relatados n?o pareciam estar relacionados ? atividade em si, mas sim ?s raz?es ou justificativas por n?o conseguirem participar das atividades. A presen?a de frases que relatavam a dificuldade de ter tempo dispon?vel foi muito presente. Conclus?o: O presente estudo evidenciou que o perfil e relato dos alunos que participaram das a??es do voluntariado da Escola de Medicina e dos que n?o participaram s?o semelhantes em diversos aspectos. Ambos os grupos consideram que a participa??o nas atividades de voluntariado ? importante para a forma??o pessoal e profissional.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-15T15:58:28Z No. of bitstreams: 1 EDUARDO_MADRUGA_LOMBARDO_DIS.pdf: 563577 bytes, checksum: 75bb1d6f556f2f712acc1396b93b1ef9 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-27T11:58:27Z (GMT) No. of bitstreams: 1 EDUARDO_MADRUGA_LOMBARDO_DIS.pdf: 563577 bytes, checksum: 75bb1d6f556f2f712acc1396b93b1ef9 (MD5) / Made available in DSpace on 2018-03-27T12:03:50Z (GMT). No. of bitstreams: 1 EDUARDO_MADRUGA_LOMBARDO_DIS.pdf: 563577 bytes, checksum: 75bb1d6f556f2f712acc1396b93b1ef9 (MD5) Previous issue date: 2017-01-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The objective of this systematic review was to answer the following question: "Does the use of glucosamine and chondroitin decrease pain and improve function in patients with osteoarthritis of the temporomandibular joint?" A search in the literature guided by PRISMA-P Statement was conducted using the PubMed, EMBASE and Cochrane Library databases and the OMNIS and VHL tools. The search terms that guided the search were: "glucosamine", "chondroitin", "temporomandibular" and "osteoarthritis". The data extraction was performed in 4 randomized clinical trials that presented according to the eligibility criteria. The selected studies were also submitted to methodological quality evaluation. Final considerations: Although RCTs show positive results regarding chondroprotective agents, there is a need for more methodological rigor in their construction. / O objetivo do projeto desta revis?o sistem?tica foi responder a seguinte quest?o: ?o uso da glicosamina e da condroitina diminui a dor e melhora a fun??o em pacientes acometidos por osteoartrite da articula??o temporomandibular??.Uma busca na literatura norteada pelo PRISMA-P statement foi conduzida utilizando as bases de dados PubMed, EMBASE e Cochrane Library e as ferramentas OMNIS e BVS. Os termos de busca que orientaram a busca foram: ?glucosamine?, ?chondroitin?, ?temporomandibular? e ?osteoarthritis?. A extra??o de dados foi realizados em 4 ensaios cl?nicos randomizados que apresentaram-se de acordo com os crit?rios de elegibilidade. Os estudos selecionados ainda foram submetidos ? avalia??o da qualidade metodol?gica. Considera??es finais: Embora os ECRs apresentem resultados positivos a respeito de agentes condroprotetores, h? necessidade de maior rigor metodol?gico na sua constru??o.