In the discourse of crime and place, the focus has rarely fallen on rural crime. While experiencing comparably lower crime levels than urban counterparts, the common association with rural areas as being symbols of peace and friendly social interaction is not necessarily accurate. One group that often are thought of as inherently rural is farmers, who’s workplaces may possess certain unique vulnerabilities to crime compared to other locations. This thesis aims to obtain a better understanding of the threats against farmers related to animal production in a Swedish context, adding to the knowledge base regarding rural crime and sustainable development of rural and urban areas. The objectives of the study are: • to investigate the nature of the victimization of farmers devoted to animal production in Sweden, especially related the situational conditions of farms and rural areas. • to explore new data that could be used to approximate the scale of the threats against farmers using data from media archives from 2009 to 2019. This study reports types, frequency, and location of crimes against animal production with a focus on mink, rabbit and pig farms. Geographical Information Systems (GIS) is used to report the geography of these offences at municipal level. The theories of the routine activity approach and situational crime prevention are used to try to explain certain conditions that may facilitate crime on farms, while the offenders are explored using the theory on techniques of neutralization. The findings of the study show that the experience of the chosen actor’s varied greatly, but with crimes such as trespassing, vandalism and theft being common types of offenses across the board. The locations of the crime events were focused in the southern to mid of Sweden. Situational conditions that may have facilitated crime includes: the large size of farms and low population density providing low detection of crime, high value targets, and relatively high accessibility to the farms. From the data, mainly three techniques of neutralization were observed to be utilized: denial of the victim, denial of injury and appeal to higher loyalties. Multiple techniques were observed to be utilized together, while simultaneously exploiting situational conditions to facilitate the neutralization.

This study was conducted to evaluate if Polonite® could remove Phosphorus from Sandvik’s wastewater that naturally forms during steelmaking, and hence be proficiently used as a precursor of the neutralization process of said steel plant. Currently lime (CaO) is mixed with the wastewater which creates clean water and sediments, that later need to be landfilled. Due to the high Phosphorus content in the sediments these cannot be recycled back into the production, this result in a loss of important elements. The aim is therefore to remove the Phosphorus to a target level of 1-2 [mg/L]. In fact, the Phosphorus present in the wastewater drifts in the solid particles derived by the neutralization process, impairing their possible recirculation as slag builders. To evaluate the purifying abilities of Polonite® a batch test and a column test were conducted. During the column test measurements of pH and conductivity were made. To analyze the composition, Inductively Coupled Plasma (ICP) tests were performed. No clear correlation between the pH and the conductivity of the water could be established from the results. Overall the Polonite® removed high amount of Phosphorus from the wastewater. The target level of 1-2 [mg/L] Phosphorus was achieved in the batch test. Although, Polonite® also incidentally absorbed most of the Fluorine present in the wastewater making it a questionable choice for the process, as Fluorine is desirable in large quantities in the sediments if used as slag builders. As no ICP results were received from the column test only pH and conductivity are discussed. / Denna studie utfördes för att utvärdera om Polonite® kunde rena Fosfor från Sandvik’s avfallsvatten som naturligt uppkommer från stålprocesserna, och använda det som ett försteg i neutraliseringsprocessen. Idag blandas kalk (CaO) med avfallsvattnet vilket resulterar i rent vatten och sediment, dessa deponeras sedan. På grund av den höga halten Fosfor i sedimenten kan dessa inte återcirkuleras in i produktionen, detta leder till att viktiga ämnen går förlorade. Målet är därför att sänka halten Fosfor till en nivå på 1-2 [mg/L]. Fosfor i avfallsvattnet driver i de fasta partiklarna från neutraliseringsprocessen, vilket förhindrar möjligheterna att återanvända dem som slaggbildare. För att utvärdera Polonites® reningsförmåga gjordes ett kolumntest och ett skaktest. Under kolumntestet mättes pH och konduktivitet. För att analysera innehållet i vattnet gjordes ICP-tester på det renade vattnet. Inget tydligt samband mellan konduktiviteten och pH kunde fastställas från resultaten. Överlag hade Polonite® en hög grad av rening av Fosfor. Den givna mängden av Fosfor uppnåddes i skaktestet. Olyckligtvis hade Polonite® även en hög reningsgrad av Fluor som även fanns i avfallsvattnet, vilket gjorde Polonite® tvivelaktigt försteg i denna process, då Fluorrikt vatten är önskat för att kunna användas som slaggbildare. Då inga ICP-resultat har mottagits diskuteras enbart pH och konduktivitet för kolumntesterna.

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Dumpen och 2forty2 är grupper som ägnar sig åt att bedriva en digital pedofiljakt och att exponera förövare på sina digitala plattformar. Syftet med denna studie var att undersöka hur Dumpen och 2forty2 på sina hemsidor och Instagram-konton dels presenterar sig själva, dels på vilka grunder man berättigar sitt agerande. Genom en kvalitativ innehållsanalys av datamaterial från Dumpens hemsida och 2forty2´s Instagram-konto har syftet undersökts. Fynden från innehållsanalysen har sedan presenterats och jämförts med tidigare forskning och för studien aktuella kriminologiska teorier. I resultaten går att urskönja hur grupperna uppkommit utifrån en misstro till rättsväsendet, omvärldens uppmärksamhet och egna upplevelser av sexuella övergrepp. Avslutningsvis presenteras en bild kring hur grupperna verkar, presenterar sig och bakomliggande orsaker, samt förslag på framtida forskning kring dessa typer av grupper.

<p>During the past fifty years, mass spectrometry, often hyphenated with chromatography, has developed into the most widely used technique for the quantitative and qualitative analysis of increasingly complex mixtures of (bio)organic molecules.</p> <p>One important aspect of this development concerns the relationship between the structure (atom connectivity) of a molecule and the mass spectrum obtained by electron ionization (EI). In this context, from 1960 - 1990, a wealth of studies has appeared that uses a variety of novel experimental techniques, often in conjunction with isotope labelling, to probe the structure, stability, reactivity and dissociation characteristics of the radical cations generated by EI of various classes of molecules. One highlight was the discovery of surprisingly stable distonic ions and the role they play in the dissociation chemistry of ionized molecules.</p> <p>However, mechanistic proposals based upon experimental observations can often only be considered as tentative. Synergy between experiment and theory would be ideal to remedy this situation, but it was not until recent spectacular advances in computer technology and software that this approach could be implemented. It has led to the growing realization that many rearrangement reactions of radical cations in the rarefied gas-phase involve catalysis. Proton-transport catalysis (PTC) is a prime example : here, a neutral species induces an ion to isomerize via hydrogen-bridged radical cations (HBRCs) as intermediates. An exemplary case described in this thesis concerns the ion-molecule reaction of the cyanamide ion with a single H₂O molecule : experiment and theory indicate that the H₂O molecule catalyzes the swift transformation of NH₂-CN^·⁺ into the more stable carbodiimide ion HN=C=NH^·⁺.</p> <p>The thesis exploits the synergy of tandem mass spectrometry and computational chemistry to study the role of catalysis in the association and dissociation reactions of several systems of radical cations. During these studies, a new type of a catalyzed reaction was discovered: "ion-catalysis", where an organic cation promotes the otherwise prohibitive rearrangement of a neutral. Ion-catalysis is proposed to explain the unexpected loss of NH₂O^· from low-energy N-hydroxyacetamide ions CH₃C(=O)NHOH^·⁺ : the molecular ion rearranges into the HBRC [O=C-C(H₂)--H--N(H)OH]^·⁺ whose acetyl (cation) component catalyzes the transformation NHOH^· --> NH₂O^·. Another highlight involves a hybrid reaction, in which both the ion and the neutral component of an incipient HBRC catalyze one another to rearrange into more stable isomers.</p> <p>Catalysis may also play an important role in astrochemistry and a question addressed in this context is whether pyrimidine, a key component of DNA, may be generated by ion-molecule reactions. It appears that the acrylonitrile ion (AN) does not react with HCN to produce ionized pyrimidine, instead it isomerizes by PTC. However, the reaction of the ion with its neutral counterpart does not involve catalysis, but rather cyclization into the pyrimidine ion ! A related topic concerns the structures of covalently bound dimers of the ubiquitous interstellar molecules HCN and HNC. Neutralization-Reionization Mass Spectrometry in conjunction with model chemistry calculations leaves little doubt that the elusive dimers HN=C=C=NH and HC=N-C=NH are kinetically stable in the rarefied gas-phase, whereas HC=N-N=CH is not.</p> <p>The structure of ions may also be probed by interactions with selected neutral molecules rather than dissociative collision experiments (MS/MS). An exciting case involves the differentiation of isomeric heterocyclic ions by ion-molecule reactions with dioxygen. Here, too, model chemistry calculations play an essential role in understanding the mechanism and the scope of the reaction.</p> / Doctor of Philosophy (PhD)

Information har idag kommit att bli så viktigt att det av många aktörer kallas för den nya digitala oljan, och med anledning av just detta är information idag en av de främsta tillgångar en organisation kan besitta. För att skydda informationen lägger organisationer massiva summor pengar på tekniska och fysiska åtgärder. Tillsammans med dessa åtgärder utfärdas även interna bestämmelser och riktlinjer för hur IT-system och information får eller inte får hanteras. Trots detta sker både intrång och andra säkerhetsrelaterade incidenter som kan härledas till mänskligt felaktigt beteende, eller den så kallade mänsklig faktorn. I den här uppsatsen har därför författaren gjort en djupdykning i ämnet för att studera vilka samband som kan finnas mellan beteendevetenskapliga teorier och efterlevnad av informationssäkerhet. Med kvalitativa metoder har bland annat litteraturstudier genomförts för att ta reda på vilka teorier som är mest relevanta i sammanhanget. Intervjuer har sedan nyttjats för att bredda författarens uppfattning om vilka faktorer som kan påverka mänskligt beteende. De personer som intervjuats har bland varit yrkesverksamma som säkerhetschefer, säkerhetskyddshandläggare och ledande forskare inom det specifika området.

Le virus Epstein-Barr (VEB) est fortement associé au développement de syndromes lymphoprolifératifs (SLP) en greffe pédiatrique. Ce virus a la capacité d’immortaliser les lymphocytes B et de provoquer leur prolifération incontrôlée chez l’hôte immunodéprimé. Plusieurs études démontrent que le cycle lytique du virus jouerait un rôle primordial dans la genèse des SLP en produisant des particules virales pouvant infecter les cellules B adjacentes. Chez un individu immunodéprimé, ces cellules B nouvellement infectées peuvent donner naissance à une expansion lymphocytaire. Le projet présenté dans ce mémoire fait partie d’un programme de recherche visant à élucider le rôle de l’infection productive par le VEB dans le développement des SLP. L’objectif précis de ce projet est de développer un anticorps monoclonal chimère contre la glycoprotéine gp350 du VEB dans le but de neutraliser le virus et d’ainsi prévenir son entrée dans les cellules B. Notre laboratoire a construit une version chimère de l’anticorps monoclonal murin 72A1, lequel se lie à la gp350 et bloque l’infection. Les premiers essais ont révélé la présence de chaînes non fonctionnelles (aberrantes) dans l’hybridome produisant l’anticorps 72A1. La construction de la chaîne légère authentique est maintenant complète alors que celle de la chaîne lourde est toujours en cours. Le processus de caractérisation de l’anticorps chimère inclura des essais de cytotoxicité à médiation cellulaire dépendante des anticorps (ADCC). Dans cette optique, une lignée cellulaire exprimant de façon stable la gp350 a été établie. Notre anticorps chimère anti-gp350 pourrait éventuellement être utilisé comme thérapie préventive chez les greffés présentant un risque élevé de SLP en empêchant l’infection des cellules B adjacentes. / The Epstein-Barr virus (EBV) is associated with B-cell post-transplant lymphoproliferative disease (PTLD). EBV has the unique property of immortalizing B lymphocytes, thereby causing their uncontrolled proliferation in an immunocompromised host. Certain evidence suggests that EBV productive infection may play a primary role in the genesis of PTLD by generating virus particles which can infect bystander B cells. In an immunocompromised individual, these infected B cells may then give rise to expanding B-cell clones. The project presented in this thesis is part of a research program seeking to elucidate the role of EBV productive infection in the genesis of PTLD. The specific aim of this work was to design a chimeric monoclonal antibody against the EBV envelope glycoprotein gp350 in order to neutralize the virus, thereby preventing entry into B cells. Our laboratory constructed a chimeric version of the murine monoclonal antibody, 72A1, which binds to gp350 and blocks infection. The initial cloning attempts revealed the presence of nonfunctional (aberrant) transcripts in the hybridoma line producing the 72A1 antibody. The chimeric version of the authentic light chain is now completed while the chimeric heavy chain construction is ongoing. As part of the characterisation process for the chimeric antibody, a cell line stably expressing surface gp350 was generated. This gp350-expressing cell line will be used for antibody dependent cellular cytotoxicity assays (ADCC). This anti-gp350 chimeric antibody could be useful as a preventive therapy in transplant patients at high risk for PTLD by blocking the infection of bystander B cells.

Le virus Epstein-Barr (VEB) est un pathogène opportuniste qui a la capacité d’immortaliser les lymphocytes B et de provoquer une prolifération maligne, appelée syndrome lymphoprolifératif post-transplantation (SLP), chez les individus immunodéprimés. A l’intérieur de ce groupe, les personnes à plus haut risque sont les enfants, puisqu’ils sont à risque de développer une infection primaire par le VEB pendant leur régime d’immunosuppression post-greffe. Dans le but de développer un anticorps préventif, notre laboratoire s’est attardé au rôle du cycle lytique du VEB dans le développement du SLP. À cette fin, le premier objectif du présent projet vise à fournir la preuve expérimentale de l’existence ou non d’une phase réplicative productive pendant l’infection aiguë des lymphocytes B sanguins. Un examen des événements qui se déroulent au tout début de l’infection par le VEB tant au niveau de la réplication virale qu’au niveau de l’expression des gènes lytiques précoces et tardifs a révélé l’existence d’une phase réplicative productive pendant l’infection aiguë. Ceci a permis de justifier l’élaboration, dans notre laboratoire, d’un anticorps chimère (murin-humain) neutralisant, dirigé contre la protéine gp350 située sur l’enveloppe virale. Le deuxième objectif, quant à lui, vise à fournir la preuve expérimentale de la capacité neutralisante de cet anticorps chimère. Des essais de caractérisation in vitro ont démontré une capacité de reconnaissance de la protéine cible, notamment la gp350, et une capacité de neutralisation du virus par l’anticorps chimère. L’anticorps chimère anti-gp350 pourra faire l’objet d’essais précliniques in vivo en vue d’évaluer sa capacité à reconnaître le virus et à prévenir l’apparition de tumeurs de type SLP chez les souris SCID. Il pourrait être éventuellement utilisé, par la suite, comme traitement préemptif contre les tumeurs dans l’espoir de mieux gérer les patients à risque de développer un SLP. / Epstein-Barr virus (EBV) is an opportunistic pathogen in immunocompromised transplant patients. In these patients EBV infection can lead to malignant B-cell lymphoproliferation, called post-transplant lymphoproliferative disease (PTLD). This thesis project aimed to investigate the role of lytic EBV infection in the genesis of PTLD. The first experimental objective was to provide in vitro proof that EBV could induce productive replication upon acute in vitro infection of B cells. Data obtained through study of viral DNA replication and transcription during the first 96 hours post-infection indicate that lytic infection does occur. These results provided justification for proceeding to the second experimental objective which involved the characterization of an anti-gp350 human-mouse chimeric antibody for its capacity to recognize and neutralize EBV. Results showed that this antibody did possess neutralization activity. Further study of this anti-gp350 chimeric antibody in SCID mice is necessary in order to evaluate its in vivo efficacy against PTLD.

Objectifs. L’objectif de ce mémoire est d’améliorer les connaissances quant à l’effet des interventions policières sur la violence imputable aux bandes criminelles. À travers l’évaluation des cinq plus importantes opérations policières réalisées entre 1991 et 2008 au Québec, trois modèles conceptuels sont confrontés : 1) celui du marché qui prévoit une hausse des affrontements entres bandes rivales dont le but est de prendre possession d’un marché criminel maintenant disponible en raison du retrait d’un joueur clé, 2) celui de la dissuasion qui prévoit une baisse des violences criminelles, et 3) celui de l’attrition qui envisage une baisse des violences en raison de l’essoufflement des bandes. Méthodologie. Les données de cette étude proviennent de l’Enquête générale sur les homicides de Statistique Canada. Différents taux d’homicides furent agrégés sur une base annuelle pour différentes provinces et régions métropolitaines de recensement. Des analyses de séries chronologiques interrompues furent ensuite réalisées pour estimer l’effet des interventions policières. Résultats. L’opération Printemps 2001 est la seule intervention policière à être associée à une baisse significative des homicides reliés aux bandes criminelles. « Sans-Frontière », « Colisée » et les deux escouades Carcajou de Montréal et de Québec, n’ont produit aucun effet préventif notable. Au contraire, Carcajou Montréal et Québec ont été associées à une hausse des homicides liés aux gangs. Conclusion. Les présents résultats appuient davantage la thèse de la dissuasion que celles du marché ou de l’attrition. Afin de produire un effet de dissuasion, les résultats suggèrent que l’intervention policière doit : 1) cibler un nombre important de membres de l’organisation criminelle, 2) s’attaquer aux têtes dirigeantes, 3) assurer la neutralisation des membres de l’organisation, et 4) assurer la diffusion du message pénal auprès des délinquants concernés. D’autres études sont toutefois nécessaires pour estimer l’effet des interventions policières dans d’autres contextes. / Objectives. The objective of this dissertation is to further our understanding about the effects of police interventions on gang-related violence. Using the five most importance police strikes conducted in the Province of Quebec between 1991 and 2008, the relevance of three conceptual frameworks is compared: (1) the market thesis that predicts an increase in gang-related violence for the possession of illicit markets, following the withdrawal of a key player, (2) the deterrence/neutralization model that predicts a decrease in gang-related violence, and (3) the attrition model which predicts a gradual decline in violence (due to losses suffered by each party during a conflict). Methodology. Data come from the Homicide Surveys conducted by Statistics Canada. Various homicide rates were aggregated on an annual basis for different Canadian Provinces and metropolitan census areas. Pooled time series analyses were then conducted to estimate effects of police interventions. Results. Operation “Printemps 2001” is the sole police intervention to be associated with a significant decline in gang-related homicides. “Sans-Frontières”, “Colisée”, and both Wolverine squads, launched in the cities of Quebec and Montreal, did not produce any noticeable preventive effect. On the opposite, Wolverine Quebec was associated with an increase in gang-related homicides. Conclusion. The present results rather support the deterrence thesis than the market and attrition models. In order to produce deterrence, police interventions have to: (1) target a significant fraction of the members of the criminal organization, (2) target the leaders, (3) neutralize members of the organization, and (4) make sure that likely sanctions are known by potential offenders. Other studies are, however, required to estimate the impact of police interventions in other settings.

Este estudo in vitro teve como objetivo avaliar os efeitos moleculares do TGF-&beta;1, com diferentes períodos de suplementação, sobre a formação do fenótipo osteogênico das células MC3T3-E1, comparando-os com células tratadas com AA+&beta;-GP suplementados com Dex e/ou TGF-&beta;1, sem e com a neutralização dos receptores de TGF-&beta;1. A expressão gênica do próprio TGF-&beta;1 e Smad3 foram analisadas, bem como, a diferenciação das células osteogênicas e a biomineralização. As células tratadas com TGF-&beta;1 sem neutralização de receptores apresentam efeito inibitório nos estágios mais avançados da diferenciação dos osteoblastos e da biomineralização in vitro, mas expressarem alguns marcadores importantes envolvidos na mineralização. Observaram-se nódulos de mineral em todos os tratamentos das células que tiveram os receptores de TGF-&beta;1 neutralizados, mas houve uma diminuição na expressão de alguns genes. Os resultados confirmam a complexidade da via de sinalização do TGF-&beta;1, mostrando que existem lacunas para que seja entendido o mecanismo dessa molécula na biologia osteoblástica. / This in vitro study aimed to evaluate the molecular effects of TGF-&beta;1, with different supplementation time periods on the establishment of MC3T3-E1 cells, comparing with cells treated with AA+&beta;-GP supplemented with Dex and/or TGF-&beta;1, without or with neutralization of TGF-&beta;1 receptors. The gene expression of the TGF-&beta;1 and Smad3 were analyzed, as well as the osteoblast differentiation and biomineralization. The cells treated with TGF-&beta;1 without neutralization of receptors have had inhibitory effect on some important stages of osteoblast differentiation and biomineralization in vitro, but expressed some important mineralization markers. Mineral nodules were observed in all treatments of cells with their TGF-&beta;1 receptors neutralized, but there was a decrease in the expression of some important genes. The results confirm the complexity of the pathway signaling of TGF-&beta;1, showing that there are gaps for understand the mechanisms of this molecule in the biology of osteoblasts.