Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-22T19:02:48Z No. of bitstreams: 1 EucastilaJordaniaDeSouzaDiasAnjos_DISSERT.pdf: 1698578 bytes, checksum: 0992b6609358eccaf68381702ed84e37 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-06T20:55:39Z (GMT) No. of bitstreams: 1 EucastilaJordaniaDeSouzaDiasAnjos_DISSERT.pdf: 1698578 bytes, checksum: 0992b6609358eccaf68381702ed84e37 (MD5) / Made available in DSpace on 2017-03-06T20:55:39Z (GMT). No. of bitstreams: 1 EucastilaJordaniaDeSouzaDiasAnjos_DISSERT.pdf: 1698578 bytes, checksum: 0992b6609358eccaf68381702ed84e37 (MD5) Previous issue date: 2016-07-18 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / A pol?tica de desenvolvimento rural no Brasil tem a abordagem territorial como estrat?gia, e estabeleceu h? alguns anos a cria??o de espa?os para participa??o social diversificada, denominados colegiados ou F?runs Territoriais. Nesses espa?os deveriam estar presentes representantes de diversos segmentos da sociedade que comp?em a din?mica pol?tica dos Territ?rios: organiza??es da sociedade civil (ONGs, Sindicatos, Associa??es, Cooperativas, Movimentos Sociais, etc.) e gestores p?blicos (secret?rias municipais e estaduais, autarquias e ?rg?os federais como o MDA). Este trabalho tem como objetivo analisar as influ?ncias e estrat?gias estabelecidas pelos representantes pol?ticos considerados centrais no debate e execu??o de a??es com enfoque Territorial no Mato Grande/RN. Focalizamos as influ?ncias de suas atua??es no campo de discuss?es conformado a partir do espa?o institucionalizado de participa??o, o Colegiado Territorial, estabelecido sob a orienta??o dessa estrat?gia em 2005 enquanto inst?ncia de gest?o e controle social das a??es do Estado. Buscamos tamb?m compreender a partir das trajet?rias desses representantes, mobilizando o conceito de campos (Bourdieu, 1989,2002) como dinamizam, influenciam a organiza??o interna, de pautas e tomada de decis?es nos espa?os para representa??o (Colegiado e N?cleo Diretivo). A partir de observa??es participantes, como tamb?m de entrevistas semi-estruturadas com os agentes considerados centrais, podemos vislumbrar elementos que aproximam essas trajet?rias. Oriundos da Agricultura Familiar, os representantes centrais conseguem exercer o poder simb?lico (Bourdieu, 1989) nesse campo de debates e interesses, compartilham em suas trajet?rias pontos comuns como o tr?nsito entre movimentos sociais e organiza??es de assist?ncia t?cnica, processos que se reproduzem em um contexto de neglig?ncia das gest?es municipais e Estadual com esse debate, fator que condicionaria a reprodu??o de um ambiente de baixa participa??o social em torno da promo??o do desenvolvimento rural, logo das perspectivas trazidas pela abordagem territorial. / The rural development policy in Brazil has the territorial approach as a strategy and established a few years ago the creation of spaces for diverse social participation, called Council or territorial forums. These spaces should be attended by representatives of various segments of society that make up the political dynamics of the territories: civil society organizations (NGOs, trade unions, associations, unions, social movements, etc.) and public managers (municipal and state secretaries, local authorities and agencies federal as MDA). This work aims to analyze the relations between political representatives considered central in the debate and enforcement actions with territorial focus in Mato Grande / RN. Focus on the influence of their actions in the field conformed discussions from the institutionalized space of participation, the Territorial Council, established under the guidance of this strategy in 2005 as the body of management and social control of state actions. We also seek to understand from the trajectories of these representatives and mobilizing the concept of fields (Bourdieu, 1989.2002) as streamline, influence the internal organization, agendas and decision making in representation spaces (Council and Director Center). Building on participants made observations, as well as in semi-structured interviews with agents considered central, can be glimpsed elements that bring these trajectories. Originating Family Agriculture, the central representatives can exercise the symbolic power (Bourdieu, 1989) in the field of debates and interests and have common points in their trajectories as transit through social and service organizations movements. Processes that reproduce in a context of neglect of municipal administrations and state with this debate, a factor that would condition the reproduction of a low social participation environment surrounding the promotion of rural development, then the perspectives brought by the territorial approach.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-02T19:24:41Z No. of bitstreams: 1 2012 - Francisco Mendes Costa.pdf: 2083197 bytes, checksum: 1eb891888d937181598e092d26c4697b (MD5) / Made available in DSpace on 2017-06-02T19:24:41Z (GMT). No. of bitstreams: 1 2012 - Francisco Mendes Costa.pdf: 2083197 bytes, checksum: 1eb891888d937181598e092d26c4697b (MD5) Previous issue date: 2012-08-13 / This thesis is to analyze the role of public policy and social actors in the evolution of Bahian cocoa plantations. In order to understand the significance of social capital in the context of the topic, the research takes into account three variables to explain the evolution of cocoa plantations, public policy, social networking and prices. Through a socio historical analysis, we sought to identify factors that comprised the causes and extent of training for cocoa crop as well as the achievements obtained with the breadth of its economic and social, that stood in the constellation of the most important monocultures the country. It seeks to explain the performance of the Bahian economy was linked to cocoa crop as well as the sustained cultivation of two public policies enacted in 1930 and 1957, opportunities for serious crises of the regional economy. Policies implemented by two agencies, ICB and CEPLAC, taken as redeemers of serious crises of cocoa in times of difficulties the state and regional economy. However, the incidence of witches' broom in 1988 and the consequent lack of an effective policy for revitalization of cacao cultivation has left an unprecedented crisis, combining that between the supporters of the fragility of the situation in the capital region excels as the most important. / Esta tese tem como proposta analisar o papel das pol?ticas p?blicas e dos atores sociais na evolu??o da cacauicultura baiana. Com vistas a entender o significado do capital social na contextualiza??o do tema, a pesquisa leva em considera??o tr?s vari?veis para explicar a evolu??o da cacauicultura, pol?ticas p?blicas, redes sociais e pre?os. Por meio de uma an?lise s?cio-hist?rica, buscou-se identificar os fatores que compuseram as causas para forma??o e extens?o da lavoura cacaueira, bem como as conquistas obtidas com a amplitude de sua dimens?o econ?mica e social, que a situou na constela??o dos mais importantes monocultivos do pa?s. Procura explicar que o desempenho da economia baiana esteve atrelado ? lavoura cacaueira, assim como o cultivo sustentado em duas pol?ticas p?blicas promulgadas em 1930 e 1957, oportunidades de graves crises da economia regional. Pol?ticas executadas por dois ?rg?os, ICB e CEPLAC, tidos como redentores das graves crises do cacau em momentos de dificuldades da economia estadual e regional. No entanto, a incid?ncia da vassoura de bruxa ocorrida em 1988 e a consequente falta de uma pol?tica eficaz para revitaliza??o da cacauicultura tem deixado o cultivo numa crise sem precedentes, aliando que, entre as vari?veis favorecedoras de tal situa??o, a fragilidade do capital social na Regi?o se notabiliza como das mais importantes.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-13T19:29:54Z No. of bitstreams: 1 2012 - Sheila Maria Palza Silva.pdf: 2855051 bytes, checksum: 660e683b46464032dd86b6313252848c (MD5) / Made available in DSpace on 2017-06-13T19:29:54Z (GMT). No. of bitstreams: 1 2012 - Sheila Maria Palza Silva.pdf: 2855051 bytes, checksum: 660e683b46464032dd86b6313252848c (MD5) Previous issue date: 2012-09-17 / CAPES / This thesis discusses the government politics of the ?Frente Popular? of state of Acre (Brazil) during the period 1999-2010, facing encouraged the development of an economy based agroforestry and guided by the principle of sustainable development and the florestania and neoestrativismo concepts. We rely on studies of federal policies implemented in the Amazon region since the 1970s until the 2000s. From there, we analyze the consequences of these policies in state of Acre, with emphasis on the period between 1999-2010, which correspond the first three Frente Popular governments. We seek to examine the government?s strategy in order to promote economic and social development of the state of Acre, based on the forestry sector (especially through the practice of forest management), while seeking to reconcile support for traditional economic activities such as farming, logging and resource extraction. The thesis also analyses state policies to encourage forest timber and livestock, highlighting the experience of Development Project supported by the International Tropical Timber Organization ? ITTO ? and its social and economic implications for agroextractivist producers of Antimary State Forest, localized in Bujari municipality, next to the state capital, Rio Branco. / Este trabalho discute a pol?tica de desenvolvimento do Governo da Frente Popular do Acre implementada no per?odo 1999-2010, voltada para o desenvolvimento de uma economia de base agroflorestal e orientada pelo princ?pio do desenvolvimento sustent?vel e pelos conceitos de florestania e neoextrativismo. A partir de um estudo das principais pol?ticas federais aplicadas na Amaz?nia desde os anos setenta at? os anos dois mil, analisa os desdobramentos dessas pol?ticas no estado do Acre com ?nfase no per?odo 1999-2010, que corresponde ao per?odo em que a Frente Popular do Acre assumiu o executivo acreano. Procura examinar a estrat?gia governamental de promover o desenvolvimento econ?mico e social do estado a partir do setor florestal (especialmente atrav?s do manejo florestal), ao mesmo tempo em que busca conciliar o enfrentamento do avan?o do desmatamento das florestas acreanas e a manuten??o do apoio ?s atividades econ?micas tradicionais, como a pecu?ria, o extrativismo e a explora??o madeireira. Analisa as pol?ticas estaduais de incentivo ao manejo florestal madeireiro e ? pecu?ria, com destaque para a experi?ncia do Projeto de Desenvolvimento da International Tropical Timber Organization ? ITTO e suas implica??es econ?micas e sociais para os agroextrativistas da Floresta Estadual do Antimary.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-20T20:01:03Z No. of bitstreams: 1 2016 - Yamile Lined Ramos Mahecha.pdf: 3895363 bytes, checksum: 950018eb665057a1690bcde81c267eb9 (MD5) / Made available in DSpace on 2017-06-20T20:01:03Z (GMT). No. of bitstreams: 1 2016 - Yamile Lined Ramos Mahecha.pdf: 3895363 bytes, checksum: 950018eb665057a1690bcde81c267eb9 (MD5) Previous issue date: 2016-06-28 / CAPES / This research has as its main objective to carry out a comparative study of policies encouraging family farming (AF) of Brazil and Colombia, between 1990 and 2010. During this period there was significant changes in both contexts, recognizing social and political category of family farming and the emergence of specific policies for this segment. To that end, previously established five benchmarks: i) the historical trajectory and evolution of the concept of family agriculture, with emphasis on the construction and configuration in both countries; ii) social actors in the rural areas, especially those involved in the construction of the social category of family farming, and also in the creation of public policies for its promotion; iii) the institutional framework built for the management of policies encouraging family farming; iv) development programs for family farming implemented in both countries, namely: the National Program for Strengthening Family Agriculture (PRONAF) and the Financing Fund of the Agricultural Sector (FINAGRO); v) and the main policy instruments used for the promotion of family farming that act in parallel to rural credit programs. This research was conducted through the use of primary sources (government documents) and secondary (specialized literature on the subject). The emphasis of the analyzes carried out during this thesis focuses on the attempt to make a comparison of the different national contexts in which they operate to support policies on family farming. In this direction, the study demonstrates that in both countries the claims produced by the struggles of their social actors and organizations of rural reverberated directly in the character of policies encouraging the AF adopted, as these represent important advances both for Brazil and for Colombia. As a result, both countries have also strengthened the institutional support for family farming, through the creation of a diverse set of development policies in order to improve their productive performance. The analysis of this dissertation show that Brazil is distinguished by having specific policies for AF, while Colombia is characterized more by more general policies, although the government has been trying to incorporate more specific policies in recent years. In this sense, cooperation agreements between the two countries can make important contributions to the improvement of policies encouraging family farming through the comparative analysis of current experiences. / A presente pesquisa tem como objetivo principal realizar um estudo comparativo das pol?ticas de fomento ? agricultura familiar (AF) do Brasil e da Col?mbia, entre os anos de 1990 e 2010. Nesse per?odo, observou-se mudan?as significativas nos dois contextos, com o reconhecimento da categoria social e pol?tica da agricultura familiar e o surgimento de pol?ticas espec?ficas para este segmento. Para tanto, s?o estabelecidos previamente cinco par?metros de compara??o: i) a trajet?ria hist?rica e a evolu??o do conceito de agricultura familiar, enfatizando-se sua constru??o e configura??o nos dois pa?ses; ii) os atores sociais do meio rural, especialmente aqueles envolvidos na constru??o da categoria social da agricultura familiar e, tamb?m, na cria??o das pol?ticas p?blicas para o seu fomento; iii) a institucionalidade constru?da para a gest?o das pol?ticas de fomento ? agricultura familiar; iv) os programas de fomento ? agricultura familiar implementados nos dois pa?ses, a saber: o Programa Nacional de Fortalecimento da Agricultura Familiar (PRONAF) e o Fundo de Financiamento do Setor Agropecu?rio (FINAGRO); v) e os principais instrumentos de pol?ticas utilizados para o fomento da agricultura familiar que atuam em paralelo aos programas de cr?dito rural. Esta pesquisa foi realizada mediante a utiliza??o de fontes prim?rias (documentos governamentais) e secund?rias (literatura especializada sobre o assunto). A ?nfase das an?lises realizadas nesta disserta??o centra-se na tentativa de realizar uma compara??o dos contextos nacionais distintos, nos quais est?o inseridas as pol?ticas de fomento ? Agricultura Familiar. Nesta dire??o, o trabalho demonstra que em ambos os pa?ses as reivindica??es produzidas pelas lutas de seus atores sociais e organiza??es do meio rural repercutiram diretamente no car?ter das pol?ticas de fomento ? AF adotadas, assim como estas representam importantes avan?os tanto para o Brasil quanto para a Col?mbia. Como resultado, ambos os pa?ses tamb?m t?m fortalecido o apoio institucional para a agricultura familiar, mediante a cria??o de um conjunto diversificado de pol?ticas de fomento, visando melhorar o seu desempenho produtivo. As an?lises dessa disserta??o demonstram que o Brasil se diferencia por ter pol?ticas espec?ficas para a AF, enquanto que a Col?mbia se caracteriza mais por pol?ticas mais generalistas, embora o governo tenha tentado incorporar pol?ticas mais espec?ficas nos ?ltimos anos. Neste sentido, os acordos de coopera??o celebrados entre os dois pa?ses podem trazer contribui??es importantes ao aperfei?oamento das pol?ticas de fomento ? agricultura familiar, mediante a an?lise comparativa das experi?ncias em curso.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-08-01T19:49:58Z No. of bitstreams: 1 2016 - B?rbara Salvaterra.pdf: 3401226 bytes, checksum: 7fd26cacf929a454530f94e023b947a3 (MD5) / Made available in DSpace on 2017-08-01T19:49:58Z (GMT). No. of bitstreams: 1 2016 - B?rbara Salvaterra.pdf: 3401226 bytes, checksum: 7fd26cacf929a454530f94e023b947a3 (MD5) Previous issue date: 2016-04-28 / This is an exploratory study on violence reported against girls and women in the State of Rio de Janeiro, from 2009 to 2014. Recent policies and legislation, addressing the situation of violence that girls and women experience, have led some studies and placement data alarming on the subject. The gradual improvement in standardization and formulation of attendance data, through the System for Notifiable Diseases Information System (SINAN), has the potential to offer advances in the understanding of violence against girls and women, recurrence and consequent improvements to the planning and monitoring of coping actions. Thus, using the SINAN database, selected reports of violence against girls and women, and had the following objectives: I) produce descriptive profile analysis of girls and women seen, characteristics of their attackers, the reported violence and cross-referrals ; and II) to analyze the risk factors associated with the likelihood of a victim suffering recurrence of any violence. Our results revealed that are more likely to reoffend any form of violence, if the victim is already intimate partner of the author of violence, whether she is old, she is separated, if the site of aggression is residential and, divergently to the literature, if the aggressor did not use alcohol. This was an exploratory analysis, because there was huge percentage of lost information, between the variables selected from the literature. In our concluding remarks, we highlight the need for investment in professional and technical training to improve the quality of data. In addition, we recommend the creation of intersectoral and multidisciplinary observatory of monitoring violence against women, to discuss, propose responses and facilitates execution paths to improve the quality of data, monitoring, analysis and evaluation of public policies and coping services to violence against girls and women. / Esta disserta??o ? um estudo explorat?rio sobre as viol?ncias notificadas no Sistema de Informa??o de Agravos de Notifica??o (SINAN), contra meninas e mulheres no Estado do Rio de Janeiro, de 2009 a 2014. Recentes pol?ticas e legisla??es de enfrentamento ? viol?ncia contra meninas e mulheres t?m propiciado debate e an?lise sobre o tema, por?m, existem ainda muitas lacunas de conhecimento, em especial quanto aos poss?veis fatores de risco, vulnerabilidades, especificidades locais associadas e recorr?ncias. Como fonte de informa??o, a gradual melhoria na padroniza??o e formula??o dos dados de atendimento, atrav?s do SINAN, pode auxiliar-nos na compreens?o das caracter?sticas que conformam o fen?meno da viol?ncia contra meninas e mulheres, e consequentes avan?os para o planejamento e monitoramento das a??es de enfrentamento. Assim, utilizando o banco de dados do SINAN, selecionamos as notifica??es de viol?ncias contra meninas e mulheres, e tivemos como objetivos: I) produzir an?lise descritiva do perfil das meninas e mulheres atendidas, de caracter?sticas de seus agressores, das viol?ncias notificadas e encaminhamentos intersetoriais; e II) analisar os fatores de risco associados ? probabilidade de recorr?ncia de qualquer tipo de viol?ncia. Nossos resultados revelaram que s?o maiores as chances de se repetir qualquer forma de viol?ncia se a v?tima j? ? parceira ?ntima do autor da viol?ncia, se ? idosa, se est? separada, se o local da agress?o ? residencial e, divergentemente da literatura, se o agressor n?o fazia uso de ?lcool. Essa foi uma an?lise explorat?ria, pois havia enorme percentual de perda de informa??o entre as vari?veis, selecionadas a partir da literatura. Em nossas considera??es finais ressaltamos a necessidade de investimentos em forma??o profissional e capacita??o t?cnica para a melhoria da qualidade dos dados. Adicionalmente, recomendamos a cria??o de observat?rio intersetorial e multidisciplinar de vigil?ncia da viol?ncia contra mulher, que discuta, proponha respostas e facilite caminhos de execu??o para a melhoria da qualidade dos dados, monitoramento, an?lise e avalia??o das pol?ticas e servi?os p?blicos de enfrentamento ? viol?ncia contra meninas e mulheres.

The goal of the thesis was to design and create one-way speed controler for brushless DC motors, that is used in air modeling. The function is based on the without sensors control method and circuit is controlled by the Atmel AVR. Power unit for motor is used Li – pol accumulator. The controller is equipped with a BEC to power the receiver and allows monitoring current and voltage on the accumulator. The measured data can be uploaded to a PC.

The goal of the thesis was to design and create one-way speed controler for brushless DC motors, that is used in air modeling. The circuit is controlled by the Atmel AVR. As power unit for motor is used Li–pol accumulator. The controller is equipped with BEC to power the receiver and allows monitoring of current consumption and voltage on the accumulator. Measured data can be upload to a PC where they can be displayed by RegulatorPC in graphical form.

Les ARN polymérases sont des acteurs indispensables de la transcription. Chez les eucaryotes il existe trois ARN polymérases (I, II et III). La ARN polymérase III (Pol III) possède 17 sous-unités, dont une qui existe sous deux formes: RPC32α et RPC32β. Seulement une des deux formes peut être intégrée dans la Pol III, créant ainsi deux polymérases différentes Pol IIIα et Pol IIIβ. Alors que RPC32β est présent dans les cellules somatiques, RPC32α est exprimé surtout dans des cellules souches et des cellules tumorales. Aujourd’hui rien n’est connu sur leurs rôles respectifs. Le cancer du sein est un problème majeur de santé publique car c’est le cancer féminin le plus fréquent. Plusieurs types de cancer du sein sont identifiés selon la présence ou absence de certains récepteurs hormonaux. Des cancers qui testent négative pour le récepteur d’oestrogène et de progestérone et qui ne surexpriment pas le récepteur pour les facteurs de croissance épidermiques humains 2 (HER2) sont appelés triple-négative. Ils ont un pronostique peu favorable, due à l’agressivité de ce type de cancer et un manque de thérapie cibles. Pour étudier le rôle de RPC32α il fallait identifier un model tumorale. En collaboration avec Jean-Paul Feugeas (INSERM UMR 1098) une étude transcriptomique a été fait sur 2627 échantillons cliniques de tissus de sein. L’étude montre que RPC32α est surexprimé dans les cancers triple-négative, alors que son homologue RPC32β est surexprimé dans les tissues normaux. Une analyse sur six lignées de cancer du sein et une ligné non-tumorale ont pu confirmer les résultats de l’analyse transcriptomique. Le modèle de cancer du sein a donc été validé. Une caractérisation des différentes lignées de cancer du sein a démontré que d’autres sousunités de la Pol III n’étaient pas surexprimées dans les cancers triple-négative. La surexpression de RPC32α n’était donc pas une conséquence d’une hyperactivité de la Pol III. Une analyse des transcrits synthétisé par la Pol III a montré que en générale les transcrits de la Pol III étaient plus fortement exprimé dans les cancers triple-négative que dans d’autres cancers. Afin d’étudier l’implication de RPC32α dans les phénomènes de tumorisation, plusieurs lignées cellulaires dépourvues de RPC32alpha ont été créé utilisant la technique CRISPRCAS9. L’absence de RPC32α n’a pas induit une augmentation de transcription ni de l’ARN de 4 RPC32α, ni de celle de RPC32β. Il n’existe donc pas de boucle de rétroaction pour RPC32α et les deux homologues ne sont pas co-régulés. Plusieurs, mais pas tous les transcrits synthétisé par la Pol III ont une expression fortement baissé dans les lignées mutants. Le fait que pas tous les transcrits ne soit affectés par la perte de RPC32α, indique qu’il existe une spécificité de transcription pour Pol IIIα et Pol IIIβ. Les cellules des linges mutants ne présentaient pas de phénotype différent des cellules mères et la croissance était la même dans toutes les lignées. Par contre les tests de croissance en agar-mou ont révélé que les lignées mutants formaient 85% de moins de colonies, indiquant que RPC32α est nécessaire pour la croissance tumorigénique in vitro. Pour tester l’effet de la perte de RPC32α sur la croissance tumorigénique in vivo, des cellules mutants et des cellules mères ont été injecté dans des souris. Les souris greffées avec des cellules mutantes montrent un départ de tumorisation retardé. Au bout de six semaines elles avaient de tumeurs deux fois plus petit que les souris avec des cellules mères. Après ablation de la tumeur primaire, les souris ont été surveillées pour l’apparition de métastases. Quatre semaines plus tard les souris greffées avec des cellules mutantes avaient 100 fois moins de métastases que les souris contrôles. Ces résultats montrent que RPC32α est nécessaire pour la tumorisation in vitro et in vivo. La protéine semble surtout jouer un rôle dans la formation des métastases, qui sont un des problèmes majeurs dans le traitement des cancers. / The RNA polymerases are key players of transcription. Eukaryotes have three RNA polymerases (I, II and III). The RNA polymerase III (Pol III) has 17 subunits, one of which exists in two alternative forms: RPC32α and RPC32β. Only one of the two forms can be integrated into the enzymes, thus generating either Pol IIIα or Pol IIIβ. While RPC32β is found in all somatic cells, RPC32α is expressed in stem cells and tumor cells. To date nothing is known of their respective roles. Breast cancer is one of the major public health problems, as it is the most common cancer in women. Several types of breast cancers are distinguished, according to the presence or absence of hormonal receptors. Cancers that test negative for estrogen receptors, progesterone receptors and that do not overexpress the human epidermal growth factor receptor 2, are called triple-negative breast cancers. They tend to have a poor prognosis, due to the aggressive nature of the cancer and the lack of targeted therapies. To study the role of RPC32α, a tumor model needed to be identified. In collaboration with Jean-Paul Feugeas (INSERM UMR 1098) a transcriptomic study was performed on 2627 clinical breast tissue samples. The study showed that RPC32α was overexpressed in triplenegative breast cancer, whereas RPC32β was overexpressed in normal tissue. A study on six breast cancer cell lines and one non-tumorigenic line confirmed the results of the transcriptomic study. The breast cancer model was thus validated. A characterization of different breast cancer cell lines showed that other Pol III subunits were not overexpressed in triple-negative breast cancer. The overexpression of RPC32α was therefore not a mere consequence of a Pol III hyperactivity. An analysis of the transcripts synthesized by Pol III showed that overall the Pol III transcript levels were elevated in triplenegative breast cancer compared to other breast cancer subtypes. In order to study the role of RPC32α in tumorigenesis, several RPC32α knock-out cell lines were created using CRISPR-Cas9. The loss of RPC32α did not induce an increase in transcription of the RNAs of RPC32α or RPC32β. This shows that no feed-back loop exists for RPC32α and that the two homologues are not co-regulated. Various Pol III transcripts showed decreased expression levels in the knock-out cell lines. Yet not all transcripts were reduced in the absence of RPC32α. This indicates that some sort of transcription specificity must exist for Pol IIIα and Pol IIIβ. The knock-out cell lines did not show any alterations in their phenotype or growth rates. However, in soft agar assays the knock-out cell lines produced 85% less colonies than the mother cell line. This proves that RPC32α is necessary for tumorigenic growth in vitro. To find out if RPC32α was also necessary for tumorigenic growth in vivo, knock-out and wild type cells were injected into mice. The mice grafted with knock-out cells showed a slowed onset of tumor growth. After six weeks, the mice injected with knock-out cells had tumors half the size of the mice injected with wild type cells. The primary tumor was ablated and mice were tracked for metastasis. Four weeks later, mice injected with RPC32α knock-out cells had 100 times less metastasis than the control group. These results show that RPC32α is necessary for tumorigenic growth in vitro and in vivo. The protein seems also to be implicated in the formation of metastasis, which are one of the greatest problems in cancer treatment today.

Contribution à l’étude du phénomène d’oscillation argumentaire. L’oscillateur argumentaire a un mouvement stable périodique, à une fréquence proche de sa fréquence fondamentale, lorsqu’il est soumis à une excitation provenant d’une source de type harmonique, à une fréquence qui est un multiple de ladite fréquence fondamentale, et agissant de manière telle que son interaction avec le système dépende des coordonnées d’espace du système. La présente thèse étudie quelques systèmes argumentaires et essaie de mettre en évidence des relations symboliques entre les paramètres de ces systèmes et leur comportement observé ou calculé. C’est la représentation de Van der Pol qui a été utilisée la plupart du temps pour représenter l’état du système, car elle est bien adaptée à la méthode de centrage, où l'on cherche une solution sous forme d’un signal de type sinusoïdal, d’amplitude et de phase lentement variables. L’originalité de la présente thèse vis-à-vis des publications antérieures est dans la modélisation, plus proche des systèmes physiques réels, dans les développements symboliques qui donnent des représentations inédites, dans le mode de réalisation des expériences, qui utilisent toutes une visualisation de Van der Pol en temps réel, et dans l’objet de l’expérience de la poutre excitée axialement de manière argumentaire. Au cours de cette thèse, des systèmes simples à un DDL ont été modélisés, construits et expérimentés. Des relations symboliques, notamment concernant les probabilités de capture par des attracteurs, ainsi que des critères de stabilité et une solution symbolique approchée, ont été mis en évidence. Un système continu constitué d’une poutre élancée excitée axialement a ensuite été modélisé à l’aide de deux modèles et expérimenté ; toujours dans le domaine symbolique, des propriétés ont été étudiées, notamment concernant des combinaisons de plages de paramètres permettant au phénomène argumentaire d’exister / Contribution to the study of the argumental oscillation phenomenon. The argumental oscillator has a stable periodic motion at a frequency close to its fundamental frequency when it is subjected to an excitation from a harmonic source at a frequency which is a multiple of said fundamental frequency, and acting in such a way that its interaction with the system depends on the space coordinates of the system. This thesis studies some argumental systems and tries to demonstrate symbolic relations between the parameters of these systems and their observed or calculated behavior. The Van der Pol representation was used most of the time to represent the state of the system, as it is well adapted to the averaging method, where a solution is sought as a signal of sinusoidal type, with slowly varying amplitude and phase. The originality of this thesis with respect to previous publications is in the modeling, closer to real physical systems, in the symbolic developments that give new representations, in the embodiment of the experiments, which all use a real-time Van der Pol visualization, and in the object of the experiment of the beam axially excited in an argumental way. During this thesis, simple systems with one DDL have been modeled, built and tested. Symbolic relationships have been highlighted, in particular with regard to the probabilities of capture by attractors, as well as stability criteria and an approximate symbolic solution. A continuous system consisting of an axially excited slender beam was then modeled using two models, and tested; still in the symbolic domain, properties have been studied, especially concerning combinations of parameter ranges allowing the argumental phenomenon to occur

In der „Industry Research Project Week“ sollen von Student_innen innovative (technische) Lösungen entwickelt werden. Dabei handelt es sich um ein praxisnahes, interdisziplinäres Lernangebot für Student_innen im Master- respektive Diplomstudium. Die Realisierung der Projektwoche erfolgte im Rahmen einer kooperativen Lehrveranstaltung von HTW und TU Dresden. Für das kollaborative Lernen werden die Studierenden in Form gemischter Kleingruppen zusammengeführt. Unter tutorieller Betreuung arbeiten sie an konkreten Fallbeispielen und entwickeln erste Lösungsvorschläge (Prototypen) für die Praxis. Als Partner für die Projektwoche standen das Fraunhofer IWU sowie die Kunsthochschule Halle/ S. zur Verfügung.