A pesquisa clínica é considerada um método inovador em medicina clínica e essencial para o desenvolvimento de novas drogas. No Brasil, a pesquisa clínica teve um grande avanço após a publicação da Resolução 196/96 pelo Conselho Nacional de Saúde e Ministério da Saúde, que foi baseada na Declaração Helsinque e Organização Mundial de Saúde. Desta forma, a realização de estudos clínicos dentro dos padrões éticos exigidos requer infra-estrutura adequada e equipe treinada. O objetivo deste estudo foi avaliar os grupos que realizam pesquisa clínica no Brasil em relação a: qualificação profissional, conhecimento regulatório e curso em Boas Práticas Clínicas (BPC). Trata-se de um estudo transversal com investigadores (PI) e sub-investigadores (SI) que foram identificados inicialmente pelo Currículo Lattes do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). A busca utilizou palavras-chave específicas que discriminam apenas aqueles que têm experiência com ensaios clínicos. Após consentimento informado, os voluntários foram submetidos a um questionário composto de questões qualitativas e quantitativas. Foram entrevistados 100 PI e SI. As regiões brasileiras mais representativas foram Sudeste (68%) e Sul (18%). As principais instituições envolvidas foram do complexo HCFMUSP, UNIFESP e outras instituições federais. Os centros foram classificados como públicos e universitário (54%) e instituições acadêmicas e privadas (23%). Com relação aos pesquisadores, 56% dedicam até 10hrs semanais para pesquisa clínica e 73% deles exercem atividades acadêmicas. No ano passado, 35% dos entrevistados tiveram 1 ou 2 artigos científicos internacionais publicados. No que diz respeito à experiência em ensaios clínicos, 57% e 77% tinham experiência na fase II e III respectivamente. Pós graduação foi observada em 86%, sendo Doutor (62%)a maior titulação observada. Sobre conhecimento BPC, 91% alegaram conhecer, embora apenas 74% tivessem curso formal. Todas as equipes de pesquisa clínica são multidisciplinares com maior participação de enfermeiros e farmacêuticos. Com relação à equipe, 88% tinham conhecimento em BPC, embora apenas 77% tivessem cursos formais. Com relação aos estudos clínicos realizados 36%, 60% e 44% são fases II, III e IV respectivamente. O tempo para aprovação dos protocolos clínicos é de um mês em 45% dos centros. A maior dificuldade em contratar profissionais nesta área foi a falta de recursos humanos qualificados (45,3%). Os benefícios da pesquisa clínica identificados foram: intercâmbio de conhecimento (35%), benefícios dos voluntários (17,8%) e melhoria na qualidade do cuidado á saúde (5,7% ). A expectativa da pesquisa clínica no Brasil citada pelos pesquisadores foi considerada boa (60,5%), mas é deficiente em incentivo e recursos. Em conclusão, os investigadores possuem qualificação e conhecimento adequado para realizar estudos clínicos, porém, existe a necessidade de maior treinamento. Os centros em que os investigadores atuam, possuem equipe treinada e infra-estrutura adequada para realização de ensaios clínicos fase II, III e IV. A pesquisa clínica possibilita ganho de conhecimento científico, atualização sobre novas condutas terapêuticas, intercâmbio de conhecimento com outras Instituições, tanto nacionais como internacionais. / Clinical trial is considered a breakthrough method in medicine and essential to the development of new drugs. In Brazil, clinical trials increased after the publication of the resolution 196/96 by the Health National Cabinet and Health Ministry, that was based on Helsinki declaration and WHO, among other relevant clinical trial documents. Clinical trials that comply with those regulations require an appropriate infrastructure and team qualification. The goal of this study was to evaluate general clinical trial groups in Brazil: professional qualification, regulatory knowledge and Good Clinical Practice. This is a transversal study with investigators (PI) and sub investigator (SI). PI and SI data were initially obtained from Curriculum Lattes from National Advice of Scientific and Technological Development (CNPq). The selection was made by using specific keywords which would discriminate those who in fact have clinical trial experience. After informed consent, the volunteers were submitted to a questionnaire which was composed of qualitative and quantitative questions. A hundred PI and SI were interviewed. The most representative Brazilian regions were Southeast (68%) and South (18%). The main institutions involved were HCFMUSP complex, UNIFESP and others federal institutions. The centers were classified in academic public institutions (54%) and private academic institutions (23%). With regard to the investigators 56% of them dedicated 10hrs per week for clinical trial and 73% have academic activities. Last year, 35% had 1 or 2 international scientific article published. Concerning the experience in trials, 57% and 77% had experience phase II and III, respectively. Academic graduation is observed in 86% of them and the higher titrations is Doctor (62%). 91% had GCP knowledge although only 74% had a formal training. About the team, all of them are multidisciplinary with majority of nurses and pharmaceuticals. 88% had GCP knowledge although only 77% had formal training. 36%, 60% and 44% of clinical trials were in phase II, III and IV. The time for approval of clinical protocols is one month in 45%. The major difficult detected was recruitment of professionals with suitable training and knowledge (45%). The benefits cited by the investigators were: improving exchange of knowledge (35%), volunteers benefits (18%) and improvement in quality of care (6%). The expectation of clinical trials in Brazil cited by the investigators was good and increasing (60%) but is deficient in incentive and resource. In conclusion, researchers have appropriate skills and knowledge to perform clinical studies however there is still a need for training. The centers where the researches work, have trained staff and adequate infrastructure for conducting clinical trials phase II, III and IV. Clinical research provides gain of scientific knowledge, update on new therapeutic conducts, sharing knowledge with other institutions, both national and international.

INTRODUÇÃO: O instrumento escolhido para coleta de informações em uma investigação científica tem de ser capaz de traduzir, com boa precisão, a realidade estudada. Neste estudo, foi analisado o desempenho de um questionário para avaliar o consumo de drogas na prisão confrontando o relato dos entrevistados com a análise toxicológica de urina. METODOLOGIA: Foi realizado um estudo observacional transversal em setembro de 2007, numa unidade prisional masculina, localizada no estado de São Paulo. Os detentos foram entrevistados e submetidos à coleta de urina para detecção qualitativa de canabinóides e de cocaína, utilizando análise por imunoensaio enzimático. Foram selecionadas duas questões; a primeira sobre uso de drogas e, a segunda, mais específica, que identifica o padrão de uso da maconha e da cocaína na prisão. Para avaliar a capacidade destas questões em identificar corretamente os indivíduos que usam drogas na prisão, foram comparadas as respostas com os resultados de exame de urina (padrão ouro) e calculadas a sensibilidade e a especificidade. Entrevistador, período em que foi realizada a entrevista, faixa etária dos entrevistados, tempo de presídio, situação prisional, relação existente entre o delito cometido e as drogas, duração da pena atual e o resultado das análises toxicológicas da urina foram escolhidos como fatores com potencial para interferir nos resultados. RESULTADOS: Participaram da pesquisa 337 detentos, com idade média de 30,4 anos, cumprindo pena média de 10,1 anos, que estão em média há 16,7 meses no presídio e que, em sua maioria não cometeram delitos relacionados a drogas (73,3%). A prevalência obtida pela análise toxicológica da urina foi de 61,4% para maconha e 7,7% para cocaína. Combinar as questões melhorou o desempenho do questionário. Dos 260 entrevistados identificados, pelo questionário, como usuários de drogas na prisão, 191 tiveram resultado positivo na análise toxicológica da urina e 69, resultado negativo. Dos 76 entrevistados identificados como não usuários de droga na prisão, 21 tiveram resultado positivo na análise toxicológica da urina e 55, resultado negativo (Sensibilidade=90,1% e Especificidade=44,1%). A prevalência para o uso de maconha na prisão, obtida a partir das entrevistas, foi de 77,4% e, para o de cocaína, de 8,8%. Os detentos que não cometeram crimes relacionados a drogas (p=0,011) e os com resultado positivo para a análise da urina para canabinóides (p=0,028) tiveram um desempenho melhor ao responder as questões relacionadas ao uso de cocaína. Os detentos mais novos consomem mais maconha na prisão (80,6%, p=0,000). Já os reincidentes (11,4%, p=0,017) e os que estão há mais tempo no presídio (17,3%, p=0,038) destacaram-se como os que consumem mais cocaína. Os detentos primários (11,3%, p=0,028) e os com resultado positivo na análise da urina para canabinóides (10,2%, p=0,009) apresentaram frequência maior de respostas dissociadas. Apresentaram frequência menor, os que cumprem pena entre 6,33 e 14,62 anos (3,4%, p=0,025). CONCLUSÕES: A concordância entre o relato de consumo de maconha e cocaína na prisão obtida pelo questionário e o resultado do exame toxicológico foi boa para as duas drogas. Combinar as respostas apareceu como uma forma de melhorar a sensibilidade do questionário. / INTRODUCTION: The instrument used for collection of information in scientific research must be able to translate with accuracy the reality under investigation. In this study, we investigated the performance of a questionnaire in assessing drug use in prison compared with toxicological analysis of urine. METHODS: A cross sectional observational study was conducted in September 2007, in a male inmate placed in the state of Sao Paulo. The prisoners were interviewed and underwent urine collection for the detection of cannabinoids and cocaine metabolites trough toxicological analysis (enzyme immunoassay). Two questions were selected, one about drug use in general, and a second one, which was more specific and used to identify the drug consumption pattern in prison. To assess the ability of these questions to correctly identify individuals who currently use drugs in prison, the responses were compared with the urine test (gold standard) and sensitivity and specificity rates were calculated. Interviewer characteristics, total period of interview, age of respondents, time in jail, penalties conditions, relationship between the offense and drug use, total penalty time and urinalysis were considered factors with the potential to affect the results. RESULTS: 337 prisoners completed the questionnaire and provided urine samples for the study. These subjects presented a mean age of 30.4 years, an average time spent in prison of 1 year and 16.7 months, 10.1 years of total penalty time and the majority of them have not committed crimes related to drugs (73.3%). The prevalence based on urine toxicological analysis was 61.4% for marijuana and 7.7% for cocaine. When the answers to the questions were combined to the toxicological results, the assessment for drug consumption trough the questionnaire was improved. Of the 260 respondents identified by the questionnaire as a drug user in prison, 191 presented positive results for toxicological analysis and 69 negative results. Of the 76 respondents identified as non-drug user in prison, 21 presented positive results for toxicological analysis and 55 negative (sensitivity=90.1% and specificity =44.1%). The prevalence of cannabis use in prison taking into account only the interviews was 77.4% and 8.8%, for marijuana and cocaine, respectively. Prisoners who have committed crimes related to drugs (p=0,011) and those with positive urinalysis for cannabinoids (p=0,028) performed better in answering questions related to cocaine use. The younger prisoners consumed more marijuana in prison (80.6%, p=0,000) than their older counterparts. Repeat offenders (11.4%, p=0,017) and those who are in prison for longer (17.3%, p=0,038) time stood out as those who consume more cocaine. First offenders (11.3%, p=0,028) and those with positive urinalysis for cannabinoids (10,2%, p=0,009) showed higher frequency of misleading answers and, less often, those who were serving time between 6.33 and 14.62 years (3.4%, p=0.025). CONCLUSIONS: The agreement between the reporting of marijuana and cocaine consumption in prison obtained by questionnaire with toxicological essay was adequate for both use in general and recent use. Combining responses appeared as a way to improve the sensitivity of the questionnaire.

Orientador: Neusa Maria Costa Alexandre / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-19T19:03:02Z (GMT). No. of bitstreams: 1 Coluci_MarinaZambonOrpinelli_D.pdf: 4250764 bytes, checksum: be2e8618076ba3c13c7db688dd4fe2dc (MD5) Previous issue date: 2012 / Resumo: Os trabalhadores da área de saúde, destacando-se os de enfermagem, são um grupo de risco para o desenvolvimento de distúrbios osteomusculares. Os objetivos desta pesquisa foram desenvolver um questionário que avalia a percepção dos trabalhadores de enfermagem sobre os fatores do trabalho que podem contribuir para sintomas osteomusculares, avaliar suas propriedades psicométricas e analisar os resultados dos instrumentos de medidas. O desenvolvimento do questionário seguiu normas metodológicas de construção e adaptação de instrumentos. Foi realizada uma revisão na literatura, identificação de instrumentos de medida e realização de entrevistas com uma amostra da população alvo da pesquisa. Os domínios, itens e o instrumento como um todo tiveram seu conteúdo avaliado por dois comitês de especialistas. Após a validade de conteúdo, foi realizado um pré-teste. Os dados foram obtidos por meio de entrevistas junto a 370 trabalhadores de enfermagem de dois hospitais. A coleta de dados foi realizada a fim de se avaliar as qualidades psicométricas da versão final denominada "ErgoEnf - Questionário para levantamento de risco ergonômico em trabalhadores de Enfermagem", sendo composta por 32 itens distribuídos em quatro domínios. A validade de constructo foi analisada por análise fatorial, pela técnica dos grupos conhecidos (com trabalhadores de enfermagem e da área administrativa) e pela validade convergente, correlacionando os resultados do instrumento com a Escala numérica de dor, com o SF- 36 e com o Questionário de avaliação do desempenho no trabalho (WRFQ). A confiabilidade foi verificada por meio da consistência interna e da estabilidade (testereteste). Foi realizada análise descritiva e avaliação da validade de constructo por meio da técnica de análise fatorial confirmatória, do teste de Mann-Whitney para comparação entre os grupos conhecidos e do coeficiente de correlação de Spearman para correlacionar os escores do novo questionário com os demais instrumentos de medidas. A consistência interna foi avaliada por meio do alfa de Cronbach. Foi estimado o Coeficiente de Correlação Intra-classe (CCI) para avaliar a estabilidade da medida. Os resultados indicaram que a análise fatorial confirmatória foi o modelo fatorial mais adequado uma vez que apresentou todos os índices de ajustes satisfatórios, confirmando a estrutura pré-definida do questionário. Verificou-se também uma diferença significativa (p < 0,01) entre as respostas dos trabalhadores de Enfermagem e da área administrativa em todos os domínios do ErgoEnf, apontando para evidência de validade discriminante desse instrumento. As correlações entre o novo questionário e a Escala Numérica de Dor e domínios do SF-36 e do WRFQ foram significativas (p < 0,01) e a maior parte delas de moderada magnitude. O alfa de Cronbach variou de 0,89 a 0,91. O CCI oscilou entre 0,64 e 0,76, o que indica estabilidade da medida. A média dos valores dos domínios do ErgoEnf mostrou que os trabalhadores de Enfermagem percebem os fatores biomecânicos exercendo uma influência moderada para o surgimento de sintomas osteomusculares, enquanto os fatores ambientais, organizacionais e psicossociais como uma influência mínima relacionada aos sintomas. Portanto, o processo de desenvolvimento e validação do "ErgoEnf - Questionário para levantamento de risco ergonômico em trabalhadores de Enfermagem" evidenciou resultados que indicam qualidades psicométricas adequadas do questionário, permitindo assim que este possa ser utilizado em estudos ergonômicos com trabalhadores de equipe de Enfermagem / Abstract: The health care workers are a risk group for developing musculoskeletal disorders, especially nurses. The objectives of this research were to develop a questionnaire that evaluates the perception of nursing workers to job factors that may contribute to musculoskeletal symptoms, to evaluate its psychometric properties, and to analyze the instruments' results. The development of survey methodologies followed standards of construction and adaptation tools. Literature reviews, identification of measurement instruments and interviews with a sample of the survey's target population were performed. The development stages of domains, items and the instrument as a whole had its content assessed by two expert committees. After the content validity, a pre-test of the questionnaire was performed. Data were obtained through interviews with 370 nursing workers of two hospitals. Data collection was carried out in order to evaluate the psychometric qualities of the final version: "ErgoEnf - Questionnaire to evaluate ergonomic risk in nursing", which was composed by 32 items distributed in four domains. The construct validity was analyzed by the factorial analysis, the technique of the known groups (nursing workers and administrative workers) and by the convergent validity, comparing the results of the instrument with the answers to the Numeric pain scale the SF- 36, and the Work Role Functioning Questionnaire (WRFQ). Reliability was assessed through internal consistency and stability (test-retest). Different quantitative analysis were performed, i.e., descriptive analysis, technique of confirmatory factor analysis, the Mann- Whitney test to compare known groups, the Spearman correlation test to correlate the new questionnaire scores with other measurement instruments, Cronbach's alpha to study the internal consistency, and the Intra-class Correlation Coefficient (ICC) to investigate the stability. The confirmatory factor analysis indicated that the questionnaire indices are all tweaking satisfactory, confirming the pre-defined structure of the questionnaire. There was also a significant difference (p <0.01) between the responses of nursing staff and administrative area workers in all domains of ErgoEnf. The correlations between the new questionnaire and Numeric pain scale, SF-36 and WRFQ were all significant (p <0.01) and most of them of moderate magnitude. Cronbach's alpha calculated to verify the instrument's internal consistency ranged from 0.89 to 0.91. ICC values ranging from 0.64 to 0.76 indicated the stability of the questionnaire. The mean values of the ErgoEnf domains showed that nursing workers perceived biomechanical factors exerting a moderate influence to the development of musculoskeletal symptoms, whereas environmental, organizational and psychosocial factors showed a minimal influence related to the symptoms. Therefore, the process of development and validation of the "ErgoEnf - Questionnaire to evaluate ergonomic risk in nursing" was completed with results that indicate good psychometric properties for use in ergonomic studies with nursing workers / Doutorado / Enfermagem / Doutor em Ciências da Saúde

Every year approximately a thousand of exchange students arrive to Växjö looking forimproving their university experience. The main problem is that this amount of peopleis very difficult to handle and organize. Sometimes, students don’t have enoughsupport, missing important information, missing interesting events and so on. In consequence, the students can develop a bad feeling about the universityorganization giving a bad feedback to the future potential incoming students. This is abad aspect to Linnaeus University because it is losing future students. In order to solve the problem, different surveys have been done to the internationalstudents, and even to the Växjö International Students (VIS) organization. The findings from these surveys are very interesting, and it is possible to identify andgroup different problems in order to make the solution easier. Furthermore, a good solution is found, improving the situation we were before. The solution found will fit in the hand of each student and will bring us in a better situationfor the university and their students.

La dépendance à la cocaïne est un problème de santé publique d’importance croissante. Il n’existe pas actuellement de traitement pharmacologique validé dans la dépendance à la cocaïne. Les essais thérapeutiques nécessitent une méthodologie qui permette d’évaluer l’efficacité des médicaments employés. Il n’existait pas à ce jour de mesure d’efficacité des traitements pharmacologiques dans la dépendance à la cocaïne validée en français. Nous avons mis au point et validé des questionnaires pouvant servir de mesures intermédiaires d’efficacité pour les essais pharmacologiques dans la dépendance à la cocaïne. Nous avons choisi de nous intéresser à deux types de variables intermédiaires : le craving et les symptômes psychotiques. Le craving est le besoin impérieux de reconsommer une drogue. Ce concept psychologique est un marqueur de dépendance. Nous avons conçu un questionnaire de craving en langue française : l’OCCS (Obsessive Compulsive Cocaïne Scale), en nous basant sur les travaux de plusieurs équipes travaillant dans le domaine de la dépendance à l’alcool. Nous avons validé ce questionnaire sur une population de 119 sujets cocaïnomanes suivis en centre de soins (Vorspan et al 2012). Nous avons notamment montré que les scores à ce questionnaire étaient corrélés à une mesure de craving par échelle visuelle analogique, étaient supérieurs chez les sujets dépendants par rapport aux sujets abuseurs de cocaïne, et étaient sensible au changement. Nous avons déjà utilisé le questionnaire de craving OCCS dans un essai thérapeutique en ouvert d’aripiprazole chez 10 patients dépendants du crack non schizophrènes (Vorspan et al 2008). Nous proposons également de l’utiliser pour évaluer l’efficacité d’interventions non pharmacologiques dans la dépendance à la cocaïne, comme la stimulation cérébrale profonde (Vorspan et al 2011), ou des interventions psychothérapeutiques. Les symptômes psychotiques se composent de différentes manifestations (hallucinations, idées délirantes et modifications comportementales). Nous avons choisi d’adapter en français un questionnaire évaluant les symptômes psychotiques transitoires survenant dans les quelques minutes à quelques heures suivant une consommation de cocaïne : le SAPS-CIP (Scale for Assessment of Positive Symptoms for Cocaine-Induced Psychosis). Nous avons montré que ces symptômes sont fréquents mais d’intensité variable dans une population de patients cocaïnomanes suivis en centre de soins (Vorspan et al, soumis), et qu’ils étaient sensibles au changement (Vorspan et al 2011). Il existe des hypothèses de vulnérabilité génétique à la survenue des symptômes psychotiques lors de l’usage de cocaïne. La vulnérabilité à cette complication pourrait être un facteur de protection vis-à-vis de l’acquisition d’une dépendance à la cocaïne (Brousse et al 2010). La mesure des symptômes psychotiques survenant lors de l’usage de drogue permet de modéliser une vulnérabilité pharmacogénétique vis-à-vis des addictions. Nous proposons d’utiliser ces deux mesures (OCCS pour le craving et SAPS-CIP pour les symptômes psychotiques) dans les essais thérapeutiques dans la dépendance à la cocaïne. En effet, il paraît pertinent, au regard des particularités cliniques de la dépendance à la cocaïne, de viser une réduction ou une disparition de ces deux ordres de symptômes. Une diminution du craving pourrait constituer une variable intermédiaire de l’objectif final d’obtenir une abstinence de la drogue. Une diminution des symptômes psychotiques pourrait constituer un moyen de réduction de la morbi-mortalité liée à l’usage de cocaïne. / Cocaine dependence is a growing public health concern in France. There is no pharmacological treatment validated for cocaine dependence treatment. Clinical trials require that validated methods are used to ascertain the efficacy of new drugs that are tested. There was no validated tool available to conduct pharmacological trials for cocaine dependence in French. We conceptualized and validated questionnaires that could be used as surrogate endpoints in pharmacological trials for cocaine dependence. We choose to work on two types of surrogate variables: craving and psychotic symptoms.Craving is the overwhelming desire to use a drug. It is a psychological construct that can be used as a biomarker of dependence. We designed a craving questionnaire in French named OCCS (Obsessive Compulsive Cocaïne Scale) after the work of several research teams working in the field of alcohol dependence. We validated this questionnaire in a sample of 119 cocaine addicts in a clinical setting (Vorspan et al 2012). We could demonstrate that the scores obtained on this questionnaire were correlated to those obtain on a visual analogue scale of craving. We could also demonstrate that the scores were higher in cocaine dependent that in cocaine abusing subjects. Lastly, we could demonstrate that the scores were time-sensitive. We used this questionnaire OCCS in an open-label trial of aripiprazole in 10 non-schizophrenic crack dependent patients (Vorspan et al 2008). We propose that this questionnaire could be used in clinical trials assessing the efficacy of various therapeutic interventions in cocaine dependent subjects, pharmacological treatments, but also deep brain stimulation (Vorspan et al 2011) and psychological interventions. Psychotic symptoms are composed of various phenomenons (hallucinations, delusions and behavioural modifications). We choose to adapt in French a validated questionnaire that assesses psychotic symptoms occurring between a few minutes to a few hours after cocaine intake: the SAPS-CIP (Scale for Assessment of Positive Symptoms for Cocaine-Induced Psychosis). We could demonstrate that those symptoms are frequent but of variable intensity in a sample of French cocaine addicts in a clinical setting (Vorspan et al, soumis). We could also demonstrate that those symptoms are change sensitive (Vorspan et al 2011). We hypothesized that there is a genetic vulnerability to cocaine-induced psychotic symptoms, and that being sensitive to the occurrence of psychotic symptoms could be a protective factor toward the development of cocaine dependence (Brousse et al 2010). The variability of cocaine-induced psychotic symptoms helps to conceptualize a pharmacogenetic model of drug dependence. We propose that those two questionnaires (OCCS for craving and SAPS-CIP for psychotic symptoms) could be used in clinical trial in cocaine dependent subjects. It seems very helpful indeed, knowing the clinical pattern of cocaine dependence, to design trails aimed at reducing or suppressing craving and psychotic symptoms. Reducing or suppressing craving could be a surrogate endpoint for cocaine abstinence. Reducing or suppressing cocaine-induced psychotic symptoms could reduce the morbidity and mortality associated with cocaine use.

This Master thesis deals with teaching of history of the Roma people in history lessons. The incorporation of Roma history to the curriculum is supported in the national Framework Program for Elementary Education, primarily through the cross-curricular theme of "Multicultural education". The theoretical part of this thesis consists of a brief summary of Roma history to the extent of what should be part of Elementary Education; history textbooks analysis from the perspective of Roma history; and list of other supportive educational materials. The practical part of this thesis presents a survey that was carried out within a small scale sample of secondary schools. Through the survey the current level of the 9th graders' knowledge about Roma history had been detected and subsequently a demonstrative lesson was delivered and evaluated.

Abstract The purpose of the study was to examine the relationship between nursing leadership and burnout among nurses in health care as well as the incidence of multidimensional leadership and burnout in Finnish nursing. A further purpose was to test a factor structure of Multifactor Leadership Questionnaire (MLQ) and Maslach Burnout Inventory (MBI) in Finnish health care. The empirical data were gathered by postal questionnaires from nursing staff working in university, central and district hospitals, health centers as well as psychiatric and private hospitals (n = 900, response rate 73%). In addition, a follow-up study was performed at a one-year interval (n = 100, response rate 79%). After deleting unusable questionnaires, the sample consisted of 627, and the follow-up study of 78 nurses and nurse leaders. The data were analyzed with descriptive and multivariate statistical methods. Nurse leaders proved to be transformational leaders who rewarded their subordinates, but traditional active and passive management-by-exception and laissez-faire leadership also appeared in their leadership behavior. Half of the nursing staff were moderately burned out and about one tenth were highly burned out. Nearly one fifth suffered from high emotional exhaustion. Nursing leadership has both positive and negative effects on burnout among nursing staff. Rewarding transformational leadership and active management-by-exception functioned as protecting factors, and passive laissez-faire leadership as an exposing factor. However, the results suggest that the relation between leadership and burnout is complex, affected by situational factors of leadership and the ambiguous nature of burnout. The six- and three-factor structure of the MLQ as well as the three-factor structure of the MBI were empirically supported. According to psychometric properties both instruments are well applicable to Finnish health care research. The results can be used in academic leadership education and leadership training in health care organizations, as well as to improve the quality of work life and to promote work well-being. The results can also be utilized in studies applying MLQ and MBI as well as when exploring factor structure of measures and in validation studies of instruments. / Tiivistelmä Tutkimuksen tarkoituksena oli selvittää hoitotyön johtajuuden ja hoitohenkilöstön työuupumuksen yhteyttä terveydenhuollossa sekä moniulotteisen johtajuuden ja työuupumuksen esiintymistä suomalaisessa hoitotyössä. Lisäksi tarkoituksena oli testata suomalaisessa terveydenhuollossa moniulotteisen johtajuusmittarin ja kolmiulotteisen työuupumusmittarin rakennetta. Empiirinen tutkimusaineisto kerättiin postikyselyllä yliopisto-, keskus- ja aluesairaaloiden, terveyskeskusten sekä psykiatristen ja yksityisten sairaaloiden hoitohenkilöstöltä (n = 900, vastausprosentti 73). Lisäksi toteutettiin seurantakysely vuoden intervallilla (n = 100, vastausprosentti 79). Aineistosta jouduttiin poistamaan vastauksia, ja poistojen jälkeen tutkimusjoukko muodostui 627:stä ja seurantakyselyssä 78 hoitajasta ja hoitotyön esimiehestä. Aineiston analyysissä käytettiin tilastollisina kuvailevina menetelminä ristiintaulukointia, kontingenssikerrointa, χ²-testiä, Pearsonin tulomomenttikorrelaatiokerrointa, t-testiä, Mann-Whitney U-testiä, Wilcoxonin testiä, yksi- ja kaksisuuntaista varianssianalyysiä sekä Kruskal-Wallisin testiä. Monimuuttujamenetelminä olivat rakenneyhtälömallitus ja lineaarinen regressioanalyysi. Mittareiden luotettavuuden arvioinnissa käytettiin eksploratiivista ja konfirmatorista faktorianalyysiä, Cronbachin alpha -kerrointa, osiosummakorrelaatioita, osioiden välisiä korrelaatioita, Pearsonin tulomomenttikorrelaatiokerrointa ja intraclass-korrelaatiokerrointa. Hoitotyön johtajat osoittautuivat työntekijöitä palkitseviksi muutosjohtajiksi, mutta johtamiskäyttäytymisessä esiintyi myös perinteistä työntekijöiden aktiivista ja passiivista valvomista sekä välttävää johtajuutta. Eroja ilmeni johtajan iän, työkokemuksen, työtehtävän ja perustyöhön osallistumisen mukaan. Puolella hoitohenkilöstöstä esiintyi keskimääräistä ja noin kymmenesosalla voimakasta työuupumusta. Voimakkaasta emotionaalisesta väsymyksestä kärsi lähes joka viides. Hoitotyön johtajuudella on sekä myönteisiä että kielteisiä vaikutuksia hoitohenkilöstön työuupumuksen kannalta. Palkitseva muutosjohtajuus ja työntekijöiden aktiivinen valvominen toimivat työuupumukselta suojaavina tekijöinä ja passiivinen välttäminen työuupumukselle altistavana tekijänä. Tulokset viittaavat kuitenkin siihen, että johtajuuden ja työuupumuksen yhteys on kompleksinen, ja tähän vaikuttavat johtajuuden tilannetekijät ja työuupumuksen moniselitteinen luonne. Hoitohenkilöstön ikä, työllisyystilanne, työaikamuoto ja työtehtävän luonne vaikuttivat johtajuuden ja työuupumuksen yhteyteen, joka oli suhteellisen pysyvä vuoden intervallilla mitattuna. Myös johtajuus ja työuupumus osoittautuivat stabiileiksi ilmiöiksi. Johtajuusmittarin kuuden ja kolmen faktorin rakenne sekä työuupumusmittarin kolmen faktorin rakenne saivat empiiristä tukea. Mittareita voidaan pitää psykometristen ominaisuuksiensa perusteella suomalaiseen terveydenhuoltotutkimukseen soveltuvina. Tutkimustuloksia voidaan hyödyntää terveydenhuoltoalan yliopistollisessa ja terveydenhuollon organisaatioiden sisäisessä johtamiskoulutuksessa, työelämän laadun parantamisessa ja työhyvinvoinnin edistämisessä, johtajuus- ja työuupumusmittaria soveltavissa tutkimuksissa sekä laajemmin mittareiden kehittämistyössä ja validointitutkimuksissa.

Introdução: As extrassístoles ventriculares e supraventriculares (EV e ESSV) são frequentes e muitas vezes sintomáticas. O íon magnésio (Mg) desempenha um papel importante na fisiologia do potencial de ação transmembrana celular e do ritmo cardíaco. Objetivo: Avaliar se a administração do Pidolato de Magnésio (PMg) em pacientes com EV e ESSV é superior ao placebo (P) na melhora dos sintomas e densidade das extrassístoles(DES). Métodos: Estudo duplo-cego, randomizado, com 90 pacientes sintomáticos consecutivos, com mais de 240/EV ou ESSV ao Holter de 24 horas e selecionados para receber P ou PMg. Para avaliar a melhora da sintomatologia, foi feito um questionário categórico e específico de sintomas relacionado às extrassístoles. Foi considerada significante uma redução de mais de 70% na DES por hora após o tratamento. A dose do PMg foi de 3,0g/dia por 30 dias, equivalente a 260mg do elemento Mg. Nenhum paciente tinha cardiopatia estrutural ou insuficiência renal. Resultados: Dos 90 pacientes estudados, 49 eram do sexo feminino (54,4%). A faixa etária variou de 16 a 70 anos. No grupo PMg, 77,8% dos pacientes tiveram redução maior que 70%, 6,7% deles entre 50% a 70% e, somente 13,3% dos pacientes com redução menor que 50% na DES . No grupo P, 44,4% dos pacientes tiveram melhora de apenas 30% na frequência de extrassístoles (p<0,001). A melhora dos sintomas foi alcançada em 91,1% dos pacientes do grupo PMg, comparada com somente 15,6% do grupo P(p<0,001). Conclusão: A suplementação de Mg por via oral reduziu a DES, resultando em melhora dos sintomas. Estudos clínicos e moleculares são necessários para avaliar o Mg intracelular e orientar quanto às necessidades diárias deste íon, evidenciar as prováveis deficiências e esclarecer melhor como prevenir e tratar pacientes com extrassístoles sintomáticas e sem cardiopatia estrutural. / Introduction: Premature ventricular and supraventricular complexes (PVC and PsVC) are frequent and often symptomatic. The magnesium (Mg) ion plays a role in the physiology of cell membranes and cardiac rhythm. Objective: We evaluated whether the administration of Mg Pidolate (MgP) in patients with PVC and PsVC is superior to placebo (P) in improving symptoms and arrhythmia frequency. Methods: Randomized double-blind study with 90 consecutive symptomatic patients with more than 240 PVC or PsVC on 24-hour Holter monitoring who were selected to receive placebo or MgP. To evaluate symptom improvement, a categorical and a specific questionnaire for symptoms related to PVC and PsVC was made. Improvement in premature complex density (PCD) per hour was considered significant if percentage reduction was >=70% after treatment. The dose of MgP was 3.0 g/day for 30 days, equivalent to 260mg of Mg element. None of the patients had structural heart disease or renal failure. Results: Of the 90 patients, 49 were female (54,4%). Ages ranged from 16 to 70 years old. In the MgP group, 77.8% of patients had a PCD reduction >70%, 6,7% of them from 50% to 70%, and only 13.3% <50%. In the P group, 44,4% showed slight improvement, <30%, in the premature complexes frequency (p<0.001). Symptom improvement was achieved in 91.1% of patients in the MgP group, compared with only 15.6% in the P group (p<0.001). Conclusions: Oral Mg supplementation decreases PCD, resulting in symptom improvement. Clinical and molecular studies are needed to evaluate intracellular Mg and develop better targets for the daily needs of this ion, show probable deficiencies, and explain how to prevent and better treat patients with symptomatic premature ventricular, and supraventricular complexes and no apparent heart disease.

The current trend towards large-scale research projects with big quantities of data from multiple sources require robust and efficient data handling. This thesis explores techniques for automatizing research data pipelines. Specifically, two tasks related to automation within a long-term research project in cognitive neuroscience are addressed. The first task is to develop a tool for automatic transcribing of paper-based questionnaires using computer vision. Questionnaires containing continuous scales, so called visual analog scales (VASs), are used extensively in e.g. psychology. Despite this, there currently exists no tool for automatic decoding of these types of questionnaires. The resulting computer vision system for automatic questionnaire transcribing we present, called "VASReader", reliably detects VAS marks with an accuracy of 98%, and predicts their position with a mean absolute error of 0.3 mm when compared to manual measurements. The second task addressed in this thesis project is to investigate whether machine learning can be used to detect anomalies in Magnetic Resonance Imaging (MRI) data. An implementation of the unsupervised anomaly detection technique Isolation Forest shows promising results for the detection of anomalous data points. The model is trained on image quality metric (IQM) data extracted from MRI. However, it is concluded that the site of scanning and MRI machine model used affect the IQMs, and that the model is more prone to classify data points originating from machines and institutions that have less support in the database as anomalous. An important conclusion from both tasks is that automation is possible and can be a great asset to researchers, if an appropriate level and type of automation is selected.

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