Rapporten behandlar utvecklingen av en portal som är tänkt att användas av alla olika delar av en företagskoncern, både som intranät, extranät och extern webbplats. Denna rapport undersöker vad som är viktigt att uppfylla för att skapa en portal av detta slag där kraven på flexibilitet är stora, samtidigt som de olika företagen i koncernen ska ha utrymme att agera både som enskilda företag och som medlemmar i en koncern. Rapporten undersöker även om tekniken SharePoint kan vara lämplig att använda i ett sådant fall samt tar upp alternativ till SharePoint. Slutsatserna är att två aspekter är särskilt viktiga i en portal av slaget som efterfrågas. Dels att portalen utformas med en tydlig struktur och väl definierade nivåer med klara uppgifter, dels att tekniken bakom portalen är anpassningsbar, öppen för vidareutvecklingar och att tekniken är modulbaserad. SharePoint uppfyller dessa aspekter och bedöms därför som lämplig att använda i en sådan portal. I den portal som rapporten avser bedöms även SharePoint vara lämpligare än de alternativa tekniker som rapporten behandlar.

A new reality, where human labor is replaced by automated machines is causing production management to rethink how they can steer the production to meet their customers demand. There is a gap of values, techniques and tools to communicate with the processes existing in the new modern factories, where data sometimes is the single output. This thesis purpose was to develop a model out of Lean and Six Sigma, as an answer to how modern factory could work with an information system, reaching the ideal state.   Through a theoretical analysis, Lean and Six Sigma differences in values, techniques and tools were weighted by the ideal state and recommendations from literature. Through a unstructured interview and system design review with a logistic group at a Swedish Modern Factory (SMF) a concept of an information system was developed. A project to test the model was initialized, where Define, Measure and Analyze phase was conducted. The model was thereafter modified from the projects implications.   The result was a Lean Six Sigma model which values are customer focus, ideal state, result orientation, committed leadership, education and involvement. The technique follow a DMAIC cycle and continuous improvements through a current to future state approach. The used techniques were considered Six Sigma heavy, where Leans principles are considered into the models values. The information system supports the model in setting the processes of the factory in either stability or potential state, where they differ in Measure and Analyze phase. The test at SMF developed several assignable causes to variation on the component As lead time. The model adapted after the test to involve a Measurement System Analysis (MSA), before setting the next current state of the process. / En ny verklighet där människor byts ut mot automatiska maskiner får produktionstekniker att fundera hur det ska styra produktionen för att möta kunders behov. Det finns en brist av värderingar, tekniker och verktyg till att kommunicera med processer i de moderna fabrikerna, där data ibland är det enda att analysera. Examensarbetets syfte var att utveckla en Lean och Six Sigma modell, som ett svar för hur moderna företag kan arbeta med ett informationssystem, för att nå det ideala stadiet.   Genom en teoretisk analys har Leans och Six Sigmas skillnader blivit vägda mot rekommendationer från litteraturen och det ideala stadiet. Genom en ostrukturerad intervju och en genomgång av systemets design hos en logistikgrupp, på ett svenskt företag (SMF), har ett koncept av ett informationssystem framtagits. Ett projekt för att testa modellen påbörjades, där Define, Measure och Analyze fasen var genomförda. Modellen blev därefter modifierad genom de implikationer projektet stött på.   Resultatet av den teoretiska analysen gav en Lean Six Sigma modell, vilka värdering är kundfokus, ideala stadiet, resultatorientering, engagerat ledarskap, utbildning och medverkande. Tekniken följde en DMAIC (Define, Measure, Analyze, Improve, Control) cykel och kontinuerliga förbättringar, från nuläge till ett framtida tillstånd. Teknikerna blev Six Sigma tunga, där Leans principer var beaktade i modellens värderingar. Informationssystemet var en supportfunktion av modellen och skapar en status för fabrikens processer, antingen i stabilitet eller potential stadiet, där de skiljer sig åt i mät – och analys fasen. Testet på SMF genererade flera grundorsaker till variation för komponents As ledtid. Modellen anpassades till att innehålla en mätsystemsanalys (MSA), innan nuläget av processen bestäms.

The nervous system is the most complex system in an organism. Functioning of the nervous system requires proper formation of neural cells, as well as accurate connectivity and signaling among them. While the major events that occur during these processes are known, the finer details are yet to be understood. Hence, an attempt was made to look for novel genes that could be involved in them. The focus of the present study is on CG9650, a gene that was uncovered in a misexpression screen, as a possible player in neuronal development in Drosophila melanogaster. The first chapter of the thesis reviews existing knowledge about neuronal development and function. The first section of this chapter explains in brief the formation and specification of neural stem cells, and their differentiation to neurons and glia. Sections 2 and 3 describe neuronal connectivity and signaling with respect to axon growth, synapse formation, function and plasticity. A comparison of invertebrate and vertebrate neuronal development is provided in section 4 of this chapter. This part also explains the use of Drosophila as a model for neuronal development and function. Chapter 2 describes the expression pattern of CG9650, which was characterized to gain insights into the possible role it plays during Drosophila neurogenesis.CG9650 is expressed in multiple cell types in the nervous system at the embryonic stage. Some of the cell sub-types have been identified from their morphology and position. Expression was restricted to neurons in the larval stage (except in the optic lobe, where it was expressed in precursors also), and continued in the pupal stage. No expression was seen in adults (except in the optic lobe). CG9650 has a putative DNA binding region, which bears homology to the mouse proteins CTIP1 and CTIP2, implying that CG9650 is possibly a transcription factor. In order to understand the function of CG9650, the protein was knocked down panneuronally. The resultant animals showed locomotor defects at both larval and adult stages, which have been described in chapter 3. Knock down larvae showed reduced displacement and speed of movement. The number of peristaltic cycles was also reduced in these animals but the cycle period was normal. In adults, movement was uncoordinated and righting reflex was lost, resulting in inability to walk, climb or fly. These results imply a defect in neuronal signaling. Sensory perception was unaffected in these animals. Stage specific knockdown of CG9650 indicated that the requirement for this protein is primarily during the larval stage. All CG9650-expressing neurons in the ventral nerve cord were glutamatergic, implying that its role in controlling locomotor activity is likely through glutamatergic circuits. Following up on these observations, signaling at the neuromuscular junction was assessed in CG9650 knock down animals. Chapter 4 discusses the signaling defects seen on CG9650 knock down, and the possible role of this protein. Electrophysiological recordings from Dorsal Longitudinal Muscles showed reduced and irregular neuronal firing in the knock down animals. These animals also had reduced bouton and active zone numbers. Moreover, overexpression of BRP, an active zone protein, rescued the locomotor defects caused by knock down of CG9650. Chapter 5 reports the effect of over expression of CG9650. Pan-neural over expression of CG9650 resulted in embryos with severe axon scaffolding defects, as well as aberrant neuronal and glial pattern. However, the incorrectly positioned glial cells in these embryos did not express CG9650, indicating that their aberrant positioning was probably due to incorrect signaling from the neurons. In conclusion, this study reports the requirement for CG9650, a hitherto unknown protein, in locomotor activity and signaling, thus ascribing for it a role in neuronal development and function of Drosophila melanogaster.

The aim of this diploma thesis is the design and implementation of web based individual information system for nutritional counseling organizations. This work is divided into several parts. The first part is devoted to the description and evaluation of applications available to support nutrition counseling organizations with reasoning why these applications are not very viable option. The second part is devoted to the analysis and design of the system in terms of vision, specification of functional and non-functional requirements, description of use cases, screen designs and architecture design using conceptual data schema and sitemap. This section is written according to the modified methodology MMSP. The third section demonstrates the implementation of the system on code written in ASP.NET language for three selected webpages. These pages are about dealing with the list of values and creating food recipes. The current version of the information system is added as an attachment.

Nesta tese, dois projetos distintos que usaram a técnica do Espalhamento de Luz foram desenvolvidos. Estudou-se a influência de soluções concentradas de uréia sobre agregados supramoleculares de anfifilicos (ASA\'s), através de condutimetria, supressão de fluorescência e espalhamento de luz. A uréia causou aumento da concentração micelar crítica (CMC) em todos os sistemas micelares estudados. A uréia diminuiu a seletividade da ligação iônica entre cloreto e brometo em micelas de haleto de cetil-trimetil-amônio, estudada através de supressão de fluorescência. Este aditivo causou também aumento na segunda CMC de brometo de tetradeciltrimetil- amônio (TTAB) e diminuição do peso molecular das micelas em bastão formadas. Concluiu-se, desta primeira parte, que a uréia tende a interferir nas transições de fase apresentadas em sistemas micelares, provavelmente através da combinação dos mecanismos direto e indireto. A uréia apresenta potencial para uso como aditivo para modulação das propriedades estruturais de sistemas micelares. Na segunda parte da tese desenvolveu-se um sistema, baseado em espalhamento de luz, capaz de monitorar o peso molecular de polímeros durante a polimerização. O sistema foi eficiente no monitoramento da polimerização da N-vinil-pirrolidinona, mostrando que o peso molecular do polímero formado é constante durante a maior parte da reação. As teorias atuais de cinética de polimerização não foram capazes de explicar esse comportamento. / Light scattering techniques (static and dynamic) were used to detenninate the effect of urea on supramolecular aggregates and to monitor on-line molar mass ofpolymerization reactions. For the first set of investigation it was already established that urea increases the CMC and the dissociation degree (α) of ionic micelles (CTABr, TTABr, and SDS). From fluorescence suppression studies it was found that urea diminishes the ionic binding in zwitterionic and cationic micelles as well as in cationic vesicles. Bromide and chloride ionic selectivity in cationic micelles was found to nearly disappear in the presence of urea. Light scattering (static) detenninations showed that the weight averaged molecular weight (Mw) ofboth SDS and CTABr are invariant by the presence of the denaturant. A slight increase in the excluded volume tenn (A2) was detected for CTABr in the presence of 3M urea. In parallel both A2 and the radius of gyration (γ) of two polyelectrolytes (PAA and Hyaluronic acid) were not affected by the presence of urea. Sphere-to-rod concentration transitions (2nd CMC) of TIABr micelles increased in the presence of urea and the MW of rod particles were observed to decrease. For the insoluble mixture of CTABr/Polystyrenesulfonate solubilization was achieved in the presence of 3M urea. These set of results were explained as a result of the dual effect of urea, that is, the indirect effect by changing the properties of the solvent and the direct effect by solvating the hydrophilic domain of the aggregates and contributing with a stronger dipole moment. The second set of experiments was directed towards the real-time, on-line monitoring of Mw of polymerization reactions. This new approach could be succeeded by the coupling a light scattering detector, an UV photometer and a differential refractometer on line with a HPLC system. Mw growth formed in the polymerization of N-vynil-pirrolidone was accomplished with a small inherent error. Within the studied conditions Mw reached a plateau at early polymerization stages, after that only an increase in the number of polymer kinetic chains was observed. Current kinetic models were unable to predict the observed Mw growth pattern. This system is of great importance in basic and technological applications by virtue of its on-line capability.

This thesis explores if there is a connection between municipal demographics and how often the municipality involves users with disability in the development of its online services. The thesis derives its theory from previous studies on user involvement and participation (UPI),  e-government development and county bureaucracy. The method is quantitative, with data gathering through surveys aimed at municipality representatives as well as downloaded from Statistiska Centralbyrån. The analysis consists of statistical methods and tests of analysis using the software SPSS. The result is analyzed by being compared to previous studies to find explanations for any existing relationships. The thesis has found some correlation between the size of a county’s population and its user involvement, when developing public digital services. The thesis has also found some correlation between a county’s population size and how often they involve users with disability. Furthermore, the thesis has found some correlation between the mean of age of a county’s population and how often they involve users with disability. The study also shows a lack of awareness of the importance of user involvement. One explanation for the findings could be the bureaucracy that controls the municipal finances. / Uppsatsen undersöker om det finns ett samband mellan kommundemografi och hur ofta kommunerna involverar användare med funktionsnedsättning i utvecklingen av sina webbtjänster. Uppsatsen utgår ifrån tidigare forskning på användarinvolvering och utveckling av offentliga tjänster samt tar avstamp i principer och lagrum för kommunalt styre. Tillvägagångssättet är kvantitativt, med datainsamling genom enkätstudie riktad till kommunrepresentanter vilket kompletteras med demografisk data hämtat från Statistiska Centralbyrån. Analys baseras på statistiska analysmetoder och tester utförda i SPSS, varpå resultaten analyseras utifrån tidigare forskning för att finna förklaring till olika samband.  Uppsatsens resultat påvisar viss korrelation mellan kommunal populationsstorlek och generell användarinvolvering i kommunens utveckling av digitala offentliga tjänster. Resultatet påvisar även viss korrelation mellan kommuners populationsstorlek och användarinvolvering av individer med funktionsnedsättning. Ytterligare ett samband som påvisas är korrelation mellan genomsnittlig ålder i en kommunpopulation och kommunens användarinvolvering av individer med funktionsnedsättning. Författarna har även funnit en viss okunskap för behovet av användarinvolvering och att den rådande finansieringsprincipen som gäller i Sverige kan vara ett par av förklaringarna bakom de identifierade sambanden.

Das Nervensystem von Drosophila melanogaster entwickelt sich aus dem Neuroectoderm entlang der anterior-posterioren Axe des Embryos. Dieses Gewebe unterteilt sich in drei Expressionsdomänen, die von ventral nach dorsal durch Expression der drei Homöobox-Transkriptionsfaktoren vnd, ind und msh charakterisiert sind. Vnd und Ind wurden als notwendig und ausreichend beschrieben, um die Zellidentitäten ihrer jeweiligen Gewebe zu determinieren. Um zu untersuchen, wie diese Transkriptionsfaktoren agieren, um ihre jeweiligen Zelltypen hervorzubringen, habe ich die genomweiten Genexpressionsmuster dieser verwandten Gewebe in Wildtyp-Embryos und in einer vnd-Mutante bestimmt. Ich fand heraus, dass anstelle eines Gewebes, das der IC-Domäne ähnelt, die Vnd-Mutante ein Gewebe hervorbringt, das neuronale Charakteristika zum grossen Teil verloren hat. Ich habe gefunden, dass der Transkiptionsfaktor "eyeless" bei ektopischer Expression in der VC den Vnd-Nervensystemphänotyp phänokopiert und ein ähnliches DNA-Bindemuster aufweist wie Vnd. Unsere Daten lassen vermuten, das Vnd sowohl als direkter Aktivator von VC-spezifischen Genen als auch als direkter Repressor von nicht-neuronalen Genen wirkt. Um die zeitlich-örtliche Auflösung weiter zu erhöhen, habe ich mit Nikolaos Karaiskos kollaboriert, um Einzelzell-Transkriptome von Embryos einer einzigen embryonalen Stufe zu generieren und einen Genexpressions-Atlas des frühen Drosophila-Embryos mit Einzelzellauflösung zu erstellen der die nahezu genomweite Genexpression fast jeder Zelle zu rekapitulieren. Um die Nützlichkeit der Plattform zu demonstrieren, untersuchten wir Expressionsmuster von Genen, die an der Hippo-Signalkaskade beteiligt sind. Wir prognostizierten Hippo-Signalaktivität in bestimmten Bereichen des Embryos und zeigten, dass der Hippo-Signalweg die synchronisierte Zellteilung in diesen Bereichen unterbricht. / The embryonic nervous system of Drosophila melanogaster derives from the neurogenic ectoderm, which is subdivided into three distinct domains. Several key transcription factors show expression exclusive to individual columns and endow their expression domains with characteristic identities. The genes vnd and ind, for example, are homeodomain transcription factors expressed in two columns. Both have been argued to be necessary and sufficient to confer the ventral column or intermediate column fates. To address the question how these transcription factors confer identities to their expression domains I determined the transcriptomic profile of these tissues in wild type and a Vnd mutant embryos. In order to do this, I established a protocol that allowed me to sequence the transcriptomes in developing embryos with spatio-temporal resolution. I found that upon knockout of Vnd, the ventral column largely looses its neurogenic identity rather than converting its fate, as models would predict. I identified Eyeless as a novel candidate transcription factor that shapes early nerve cord identities. Furthermore the data indicates that in Vnd acts as both, activator and repressor. Excessive co-binding with the GAGA-factor GAF suggests a mechanism by which this activation might be achieved. To push spatio-temporal resolution towards the single cell level, I collaborated with Nikolaos Karaiskos to extract single cell transcriptomes of a single developmental stage. This has allowed us to establish a digital single-cell resolved transcriptomic map of a single developmental stage. We used this map to predict expression patterns of thousands of genes with striking accuracy. We identified the Hippo signaling pathway as a spatially regulated pathway in early embryos and showed that it directs the interruption of cell cycle synchronicity in specific areas of the embryo.

Open-plan offices are widely used by companies today. There is also secluded rooms in the form of group and meeting rooms, although these are often limited in numbers. Not being able to book these rooms when needed leads to problems planning for example customer meetings. Which is also a problem for students in the school world. Today there is a number of studie rooms at Royal Institute of Technology in Kista, school for information and communication technology, where none of them are bookable. Earlier there where bookable studie rooms in the library and in other parts of the school, but such a system is no longer available because of the new facilities. The result of this is that students at the school are not able to plan their studies in an effective way because they do not know if they have a suitable place to study at between lectures or exercises. Because of this there is a need for a system that can provide students the opportunity to book a studie room. The thesis documents the development of a booking system prototype for study rooms. The prototype have been developed with HTML, PHP, PostgreSQL, JavaScript och Ajax. The thesis contains a literature studie of the current booking systems available. An analysis and an evaluation of these systems have been used as a foundation for the development. Interviews of students have been used as a data collection method. Summarizing the students opinion gave an indication of what is required by the system in terms of functionality and the user interface. The project also describes the working methods that have been used. The project resulted in a prototype for the booking system, where users can see all the available study rooms, make a booking, confirm the booking and remove their bookings. The prototype uses a database that is fully modified. The result from an evaluation of the prototype indicates what aspects that needs to be improved in future work. / I dagsläget är öppna kontorslandskap vanligt förekommande hos företag. Det finns avskilda platser i form av grupp- och mötesrum, dessa är dock allt som oftast begränsade till antal. Att inte kunna boka dessa rum vid behov leder till svårigheter att planera till exempel kundmöten. Problemet att kunna boka ett avskilt rum vid behov finns även inom skolvärlden. På Kungliga Tekniska Högskolan (KTH) i Kista, skolan för information och kommuniktionsteknik, finns det ett antal grupprum där inga av dem är bokningsbara. Det har tidigare funnits bokningsbara grupprum i biblioteket och i andra delar av skolan men idag saknas ett sådant system på grund av nya lokaler. Detta resulterar i att studenter inte kan planera sina studier på ett effektivt sätt då de inte vet om de har en lämplig studieplats mellan föreläsningar. Därför finns det ett stort behov av ett system där studenterna kan boka grupprummen. Uppsatsen dokumenterar utvecklingen av en prototyp ett bokningssystem för grupprum som gjorts med hjälp av HTML, PHP, PostgreSQL, JavaScript och Ajax. Arbetet beskriver även de arbetsmetoder som använts under arbetets gång. Uppsatsen inkluderar en undersökning av bokningssystem som finns idag. Som datainsamlingsmetod har även intervjuer av studenter använts. Utvärderingen av bokningssystemen som finns idag tillsammans med resultatet av intervjuerna gav en indikation om vad som krävs av bokningssystemets användargränssnitt och funktionalitet. Arbetet resulterade i en prototyp av bokningssystemet där användaren kan se vilka grupprum som är lediga, boka grupprum, kvittera bokningar och ta bort bokningar. Prototypen använder en databas som modellerats och implementerats. En evaluering av prototypen resulterade i vad som kan förbättras i ett framtida arbete.

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In our progressively digitized world, the escalating demand for software solutions intensifies the need for proficient developers. Low-Code Development Platforms (LCDPs) present a promising approach to address this necessity, empowering individuals without traditional programming skills to create software applications. However, despite their potential, these platforms are often not accessible or intuitive for non-professional developers. This research examines the design of LCDPs, with an emphasis on enhancing the user experience for non-programmers. By investigating the usability of LCDPs and designing a prototype based on the findings, my aim is to contribute to the ongoing discussion about the democratization of software development and to propose enhancements that could make LCDPs more user-friendly, inclusive, and usable across devices. The research involves a combination of literature review, interviews, prototype development, and user testing, providing a multifaceted perspective on the topic. Moreover, it discusses potential implications for the design of LCDPs, as well as for the broader field of interaction design.