Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-15T14:55:25Z No. of bitstreams: 2 Dissertacao_2009_Joyce_Lobo.pdf: 1055257 bytes, checksum: c6fb4445c47b40e183c76262a465da47 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Cláudia Bueno (claudiamoura18@gmail.com) on 2014-09-16T02:03:36Z (GMT) No. of bitstreams: 2 Dissertacao_2009_Joyce_Lobo.pdf: 1055257 bytes, checksum: c6fb4445c47b40e183c76262a465da47 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2014-09-16T02:03:36Z (GMT). No. of bitstreams: 2 Dissertacao_2009_Joyce_Lobo.pdf: 1055257 bytes, checksum: c6fb4445c47b40e183c76262a465da47 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2009-12-09 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Fundação de Apoio à Pesquisa - FUNAPE / Curraleiro breed was formed in regime of extensive breeding on region of cerrado, with minimal attention to infectious diseases and alimentary, resulted in animals extremely rustic that composed an important genetic recourse for Brazilian livestock. In order to improve the understanding about immunological aspects this breed, a comparative study between Curraleiro and Nelore calves after immunization with BCG vaccine. Was realized the quantity of IgM and IgG antibodies specifics to Mycobacterium bovis, by indirect ELISA in house method. Was determined twelve young calves (six month of age) were used throughout the study. The animals were divided in two groups: six calves from Curraleiro breed and six calves from Nelore breed. Each group was further divided in: immunized group which received the BCG vaccine and a control which received the adjuvant. The data were submitted to descriptive statistics and then to the Kruskall Wallis and Mann-Whitney tests. It was realized hemogram, total protein, albumin, globulin eletroforesis of serological protein, ELISA for M. bovis IgG and IgM levels. The results were grouped and Curraleiro and Nelore calves were compared, the Curraleiro breed exhibited larger numbers of leucocytes, neutrophils, lymphocytes and monocytes; higher proteins and globulins levels; and higher concentration of alfa-1, beta and gama globins fraction. So, considered the vaccination to BCG, the Curraleiro calves were more levels in produce specifics M. bovis IgM and IgG. Between the immunized groups, Curraleiro group immunized presented better performance than Curraleiro control group, Nelore group immunized and lastly Nelore control group. Concluded that Curraleiro breed compared to Nelore breed exhibited unspecific and specific humoral immune response more productive qualified for bigger numbers of leucocytes and higher concentration of unspecific and specific immunoglobulin. / A raça Curraleiro foi formada em regime de criação extensiva na região do cerrado, com mínimos cuidados sanitários e de alimentação, resultando em animais rústicos, que constituem um importante recurso genético para a pecuária brasileira. Com o objetivo de ampliar os conhecimentos sobre aspectos imunológicos dessa raça, foi realizado o estudo comparativo entre bovinos da raça Curraleiro e Nelore após vacinação com Mycobacterium bovis - BCG. Para tanto se quantificou a produção de anticorpos IgM e IgG específicos para Mycobacterium bovis, por meio do teste de ELISA indireto método in house. Utilizou-se 12 bezerros de propriedades do Estado de Goiás, seis de cada raça com idade aproximada de seis meses. Os bezerros de cada raça foram subdivididos em outros dois grupos, o grupo vacinado recebeu Mycobacterium bovis BCG via intradermica. Inicialmente os dados foram submetidos à estatística descritiva e posteriormente aos testes de Kruskall Wallis e Mann- Whitney. Para o protocolo de avaliação realizaram-se os seguintes exames: hemograma, quantificação de proteína total, albumina, globulina, eletroforese das proteínas séricas, IgG e IgM M. bovis específicas. Agrupando os resultados de todas as colheitas, observou-se que os bezerros da raça Curraleiro quando comparados aos bezerros Nelore, apresentaram maiores contagem total de leucócitos, segmentados, linfócitos e monócitos; concentrações mais elevadas de proteínas e globulinas séricas; das frações alfa-1, beta e gama globulinas. Considerando especificamente a vacinação com Mycobacterium bovis - BCG, os bezerros Curraleiro produziram maiores quantidades de IgM e IgG M. bovis específicas quando comparados aos bezerros Nelore. Entre os grupos vacinados o que apresentou maiores valores foi o grupo Curraleiro vacinado, seguido pelo grupo Curraleiro controle, posteriormente grupo Nelore vacinado e por fim o grupo Nelore controle, com as menores concentrações. Conclui-se que bovinos da raça Curraleiro quando comparados aos da raça Nelore apresentam resposta imunológica inespecífica e específica humoral maiores, caracterizada por maior número de leucócitos e maiores concentrações de imunoglobulinas IgM e IgG M. bovis específicas.

O presente estudo mensura o impacto no preço dos atributos de qualidade nos tourinhos da raça Nelore comercializados em leilões por meio de uma metodologia específica, baseada na Teoria dos Preços Hedônicos. Para tanto, utilizou-se como amostra três leilões - 368 observações - de uma fazenda padrão, o Nelore Jandaia. Assim, os atributos dos animais que mais contribuíram para a formação dos preços, em um primeiro plano, foram: a qualidade genética como um todo, expressa através do Mérito Genético Total - MGT, e a qualidade fenotípica global, demonstrada pelo índice EPMURAS. Em um segundo plano, mais específico, agregaram mais valor aos jovens reprodutores características relacionadas à precocidade e fertilidade do lado genético a Diferença Esperada na Progênie - DEP para perímetro escrotal aos 365 dias (dpe365) e no lado fenotípico a nota de precocidade. Por exemplo, os animais com MGT excelente tiveram um prêmio médio de 22% a mais em relação aos considerados como bons, paralelamente os com EPMURAS excelente tiveram um prêmio de 11% em relação aos classificados como muito bons, ceteris paribus. Para cada ponto a mais na precocidade e na DEP dpe365 o valor dos animais comercializados aumenta em, respectivamente, 5% e 5,8%, ceteris paribus. Do exposto, pode-se concluir que se os pecuaristas dedicados ao melhoramento dos seus rebanhos privilegiarem em seus objetivos de seleção animais com excelentes atributos de fertilidade e precocidade receberam prêmios superiores por isso. Em outras palavras, focar a seleção em precocidade e fertilidade agrega valor aos animais. / This study measures the impact on the price of quality attributes in Nelore steer sold at auction by a particular methodology, based on the theory of hedonic prices. To this end, we used as a sample three auctions - 368 observations of a standard farm, Nelore Jandaia. Thus, the attributes of animals that contributed most to the price formation were the genetic quality as a whole, expressed through the Total Genetic Merit - MGT, and the overall phenotypic quality, as demonstrated by the index EPMURAS. In a second model, more specific, added more value to young breeding characteristics related to precocity and fertility - the genetic side of the Expected Progeny Difference - EPD for scrotal circumference at 365 days (dpe365) and on the phenotypic note of precocity. For example, animals with excellent MGT had an average premium of 22% more than for those considered as good, along with the excellent EPMURAS had a premium of 11% over rated as very good. For each point on precocity and DEP dpe365 the value of animals traded increases, respectively, 5% and 5.8%, ceteris paribus. From the above, we conclude that if the farmers devoted to the improvement of their herds give priority in their selection goals animals with excellent attributes of fertility and precocity will receive premium for it. In other words, focus the cattle selection on fertility and precocity adds value to animals.

The dog was the first animal to be domesticated and the process started at least 15 000 years ago. Today it is the most morphologically diverse mammal, with a huge variation in size and shape. Dogs have always been useful to humans in several ways, from being a food source, hunting companion, guard, social companion and lately also a model for scientific research. This thesis describes some of the changes that have occurred in the dog’s genome, both during the domestication process and later through breed creation. To give a more comprehensive view, three genetic systems were studied: maternally inherited mitochondrial DNA, paternally inherited Y chromosome and biparental autosomal chromosomes. I also sequenced complete mitochondrial genomes to view the effect new living conditions might have had on dogs’ genes after domestication. Finally, knowledge of the genetic structure in purebred dogs was used to test analytic methods usable in other species or in natural populations where little information is available. The domestication process appears to have caused a relaxation of the selective constraint in the mitochondrial genome, leading to a faster rate of accumulation of nonsynonymous changes in the mitochondrial genes. Later, the process of breed creation resulted in genetically separated breed groups. Breeds are a result from an unequal contribution of males and females with only a few popular sires contributing and a larger amount of dams. However, modern breeder preferences might lead to disruptive selective forces within breeds, which can result in additional fragmentation of breeds. The increase in linkage disequilibrium that this represents increases the value of purebred dogs as model organisms for the identification and mapping of diseases and traits. Purebred dogs’ potential for these kinds of studies will probably increase the more we know about the dog’s genome.

Two pieces of complementary research dealing with the study of the importance of genetic factors on the quality of the Spanish dry-cured ham Jamón Serrano were carried out for this thesis. The first one compared Serrano dry-cured hams made from typical commercial lines of four representative breeds (Duroc, Landrace, Large White and Piétrain) subjected to the current standard industrial process. Most of the differences in raw material, final composition and rheology were between Piétrain and the other three breeds. Duroc breed showed the most important differences in instrumental colour and appearance, probably caused by its higher intramuscular fat content. The differences between the four breeds in dry-cured ham sensory quality were too small to discriminate them on single traits. However, according to multivariate analyses (PCA), the Large White showed the most appropriate sensory characteristics for dry-cured ham production of the four pure breeds under the processing conditions used, while the Piétrain showed the least appropriate ones. The second piece of research studied the effect of the previously identified PRKAG3 and CAST genetic polymorphisms (PRKAG3 Ile199Val, CAST Arg249Lys and CAST Ser638Arg) on the quality traits of the Spanish dry-cured ham Jamón Serrano. Associations between the polymorphisms and traits of flavour and texture were found. The PRKAG3 Ile/Ile genotype, the CAST249 Arg/Arg genotype, the CAST638 Arg/Arg genotype and the haplotype CAST 249Arg-638Arg are the most favourable for the production of the Spanish dry-cured ham Jamón Serrano. It can be concluded that the effect of different pure breeds was not strong enough to produce significant perceivable differences between dry-cured hams. In contrast, differences between genotypes of PRKAG3 and CAST polymorphisms on proteolysis and sensory traits indicated that polymorphisms could induce perceivable differences in the sensory quality of dry-cured hams. Accordingly, genetic polymorphisms could be better indicators of material screening in the dry-cured ham industry than breeds. / En aquesta tesi s’han realitzat dos estudis complementaris sobre la importància dels factors genètics sobre la qualitat del pernil curat Jamón Serrano. El primer compara pernils curats elaborats a partir de línies genètiques comerciales de quatre races representatives (Duroc, Landrace, Large White y Piétrain) i sotmesos a un procés industrial estàndard i actual Les majors diferències en els pernils frescos i en la composició i reologia final es van observar entre Piétrain i les altres tres races. La raça Duroc va presentar les principals diferències en color instrumental i aparença sensorial, probablement degut al seu alt contingut de greix intramuscular. Les diferències en característiques sensorials entre les quatre races van ser massa petites per a poder discriminar entre elles en base a característiques individuals. No obstant, considerant les anàlisis multivariades (PCA), la raça Large White va mostrar les característiques sensorials més apropiades per al pernil curat en les condicions de elaboració aquí utilitzades, mentres que la raça Piétrain va mostrar les menys apropiades. El segon estudi analitza l’efecte dels polimorfismes genètics PRKAG3 i CAST, prèviament identificats (PRKAG3 Ile199Val, CAST Arg249Lys i CAST Ser638Arg), sobre les característiques de qualitat del pernil curat espanyol Jamón Serrano. Hi va haver associacions significatives dels genotips PRKAG3, CAST249 i CAST638 i del haplotip CAST amb varis caràcters relacionats amb flavor i textura. Els genotips PRKAG3 Ile/Ile, CAST249 Arg/Arg, CAST638 Arg/Arg i l’haplotip CAST 249Arg-638Arg són els més favorables per a l’elaboració del pernil curat Jamón Serrano. Es pot concloure que l’efecte de la raça no va ser prou fort per a provocar diferències perceptibles entre els pernils curats. Per contra, les diferències entre genotips dels polimorfismes PRKAG3 i CAST poden induir diferències perceptibles en la qualitat sensorial dels pernils curats. En conseqüència, els polimorfismes genètics podrien ser millors indicadors per a la selecció de la matèria prima a les indústries elaboradores de pernil curat que les races.

The study was carried out at the Laboratory of Animal Breeding Value Establishment and Selection of Lithuanian Veterinary Academy in the time of postgraduate studies from 2003 to 2005. During the last seven years Lithuanian red and red-and-white controlled dairy cattle breeds average yield increased 41.7%, milk fat production - 50.9%, milk proteins production – 46.1%. Between milk fat and milk proteins was found medium positive correlation coefficient (rp =0.36-0.56; p<0.01), which show milk fat increasing has positive effect to milk proteins increasing. Correlation coefficient between milk production and milk fat, milk proteins is very high (rp =0.87-0.96; p<0.01). The analysis of Variance showed the breed influence on reproductive traits of cow to be statistically reliable (p<0.001). The breed had the greatest influence on cows age during calving. The influence of bulls line on many traits of cows exterior is statistically reliable (p<0,001).

Tyrimai atlikti 2008 m. LŽŪU medelyne įvertinant pirmamečių kriaušaičių, skiepytų į Pyrus pyraster Burgsd. ir Cydonia oblonga Mill. poskiepius, augimo dinamiką ir biometrinius rodiklius. Tirtos pirmamečių kriaušaičių veislės Suvenirs, Kafedralna, Kleboninė, Concorde ir Mramornaja, skiepytos į sėklinį Pyrus pyraster poskiepį ir veislė Kafedralna, skiepyta į Cydonia oblonga poskiepį. Analizuojant bandymą buvo atsižvelgiama į pagrindinius biometrinius skiepų rodiklius: aukštį, kamienėlių skersmenį, skiepų lapų masę ir lapų paviršiaus plotą. Tyrimais nustatyta, kad aukščiausi medeliai užaugo Suvenirs ir Kafedralna veislės, skiepytos į sėklinį Pyrus pyraster poskiepį. Storiausi kamienėliai buvo ΄Mramornaja΄, ΄Kleboninės΄ ir ΄Kafedralna΄ skiepytos į sėklinį Pyrus pyraster poskiepį. Mažiausi ir ploniausi - Kafedralna veislės skiepytos į Cydonia oblonga poskiepį. Intensyvus tirtų kriaušių veislių augimo tarpsnis tęsėsi nuo gegužės mėn. 30 d. iki rugpjūčio mėn. 20 d., o kamienėlių storėjimo – nuo gegužės mėn. 30 d. iki rugsėjo mėn. 10 d. Didžiausią lapų paviršiaus plotą išaugino ΄Mramornaja΄ skiepyta į Pyrus pyraster poskiepį ir veislės Kafedralna skiepyta į Cydonia oblonga poskiepį. Didžiausia lapų masė tarp kriaušaičių, skiepytų į Pyrus pyraster poskiepį, nustatyta Mramornaja ir Kafedralna veislių. / The experiment took place in 2008 in the Lithuania’s University of Agriculture evaluating the dynamic growth of first – year pear – trees on Pyrus pyraster Burgsd. and vegetatyve on Cydonia oblonga Mill. rootstock. The research grafted cv. 'Suvenirs', 'Kafedralna', 'Kleboninė', 'Concorde‘ and 'Mramornaja', grafted on Pyrus pyraster rootstock and 'Kafedralna' on Cydonia oblonga rootstock. While analyzing the attention experiment was paid to their biometrical parameters of grafts highness, stem thickness, leaves’ mass, their surface area. The research showed, that the highest pear - trees were raised from ΄Suvenirs΄ and 'Kafedralna' on Pyrus pyraster rootstock. The thickesst stems were ΄Mramornaja΄,΄Kleboninė΄ and 'Kafedralna' on Pyrus pyraster rootstock. The smallest and the slimmest stems - 'Kafedralna' on Cydonia oblonga rootstock. The most intensive period of investigation of growth continued from May 30 till August 20 th, and the period of stem thickness - from May 30 till September 10 th. The largest surface area of leaves grown ΄Mramornaja΄ on Pyrus pyraster rootstock and 'Kafedralna' on Cydonia oblonga rootstock. The largest leaves’ mass were ΄Mramornaja΄and 'Kafedralna', inoculation into Pyrus pyraster rootstock.

Tikslas: išanalizuoti įvairių veiksnių įtaką šunų babeziozės pasireiškimui, plitimui, atsižvelgiant į šuns lytį, amžių ir veislę, naudojant literatūros šaltinius bei asmeninius tyrimus. Duomenys buvo surinkti iš " Jakovo veterinarijos centras", smulkių gyvūnų klinikos "Pas Filą " ir kraujo tyrimai buvo renkami ir atliekami Veterinarijos ir diagnostikos centre. Iš viso surinkome 1195 ligos atvejų, atsižvelgiant į lytį, amžių bei veislę. Anamnezė buvo surinkta iš 117 šunų, kraujo mėginiai buvo imami iš 31 sergančio šuns, kraujo morfologiniams tyrimams. Išanalizavus esamus duomenis, pastebėjome, kad dažniausiai babezioze serga tam tikros šunų veislės: mišrūnai, vokiečių aviganiai, labradoro retriveriai ir sibiro haskiai. Atsižvelgiant į lytį, tai daugiausiai babezioze sirgo patinai ir žymiai rečiau patelės. Babezeliozė dažniausiai buvo diagnozuojama jauniems šunims, 1 iki 2 metų, bet sunkiausia klinika buvo pasireiškusi šuniukams iki 1 metų. Dažniausiai pasireiškianti klinikinė simptomatika: apatija, anoreksija, kliniškai išreikšta anemija, pakitusi kūno temperatūra, tamsus šlapimas, vėmimas, NS sutrikimai, viduriavimas, kliniškai išreikšta gelta. Analizuojant morfologinius kraujo rodiklių pokyčius, nustatėmė, kad visais atvejais buvo pasireiškusi trombocitopenija, sumažėjęs trombokrito kiekis. Pastebėjome, kad ligai būdingas sezoniškumas. Stipriausias babeziozės bumas pasireiškia pavasarį – balandžio, gegužės, birželio mėnesiais ir kiek silpnesnis – rudenį (rugsėjį, spalį). ... [toliau žr. visą tekstą] / Goal: to find out a variety of factors influence the occurrence of canine babesiosis, development, according to dog sex, age and breed, using known literature and personal research. The data were collected from „Jakovo veterinary center“, small animal clinic „pas Filą“ and the blood tests were collected and conducted at Veterinaty and Diagnostic center. We collected 1195 disease cases according to sex, age and breed. Anamnesis were collected from 117 dogs, morphological studies of blood – 31. According to the studies, we found out, that there are certain breeds are more susceptible to babesiosis: mongrels, german shepherds, labrador retrievers and siberian huskies. Also found, that babesiosis more prone males than females. Dog babesiosis were usually diagnosed in young dogs, 1 to 2 years, but the hardest clinic gets puppies up to 1 year. The most frequent clinical manifestations in patients with babesiosis : apathy, anorexia, symptomatic anemia, change in body temperature, dark urine, vomiting, NS disorders, diarrhea, clinical jaundice. Morphological characteristics of the blood we found that in all cases, is characterized by thrombocytopenia, a decrease thrombocyte. The seasonal variation in the number of cases has been reported at all Veterinary clinics, with a higher incidence in the spring months (April, May) and peaking in autumn (September, November).

abstract: The e-Retail industry has grown rapidly over the last few years and is projected to continue its upward trend as consumers shift from traditional channels to online channels. In March 2010, Forrester Research forecasted that online retail sales will grow by 10% a year for the next 5 years and e-Retail sales will amount to $249 billion by 2014. With intense competition for market share and profits, information systems and technology (IST) sourcing decisions are becoming increasingly important to e-Retail firms to support continued growth and market responsiveness. There are several aspects for e-Retailers to consider when formulating its IST sourcing strategy. Whether to choose make versus buy for technology assets and services has been addressed in both strategy and IS literature (Handfield et al. 1999, Leiblein et al. 2002, Wade and Hulland, 2004). Then there is the follow-up question of selecting a best-of-breed strategy or tighter partnership with a select group of vendors (Clemons et al. 1993, Kauffman and Tsai 2009). Few studies have looked at IST sourcing or proposed models and frameworks for evaluating IST sourcing decisions (Saarinen and Vepsalainen, 1994). Furthermore, these existing studies mainly address the antecedents of the decisions but not so much on their performance effects (Kauffman and Tsai 2009; Smith et al., 1998). The goal of this study is to extend the knowledge of IST sourcing for e-Retailers, a topic which has received limited attention (Kishore et al., 2004), by addressing a core problem: How should an e-Retailer develop and implement its IST sourcing strategy to accommodate the increase in consumer demand and IT complexity but still achieve high performance? The study introduces two theoretical models to examine organizational factors that influence an e-Retailer's IST sourcing strategies of make versus buy and partnership versus best-of-breed. The proposed models are tested using a panel data set of 307 e-Retail firms over the period of 2006 to 2010. The study opens up the black box of internal firm operations by introducing a granular view of IST sourcing decisions at both the value chain and e-Commerce architecture levels and examining the performance impacts of these strategic choices. This in-depth look at IST sourcing has yet to be explored in the literature. / Dissertation/Thesis / Ph.D. Information Management 2012

The aim of the thesis was to evaluate the data about body condition of cows and assess the relationship between changes of health status of the mammary gland, milk production and quality of cow´s milk. The monitoring took place in a selected company from October 2015 to September 2016 with the Czech spotted cattle. The body condition scoring was monitored within the herd, with the monthly evaluation of data, during the milking in the morning, together with the production performance control. The data values, received out of production performance control, were used for milk production performance (amount of milked milk, % fat, % protein, % lactose, SCC). Then the data about reproduction of the monitored herd were used, so as the order of lactation, date of the last calving and age. Total of 482 cows were monitored. During evaluation of the condition score, the average level of cows body condition was detected with value of 2, 18. Minimal body condition, during the monitored period, was 1.25, the maximum was 3.75. Correlation analysis of the relationship between the form and amount of the milked milk showed a negative correlation (-0.21). Furthermore the quality of milk was monitored. The amount of fat in milk, within the monitored herd, was 4.16% and 3.63% of protein. The fat was found to be the component, which the cow´s form had the effect on, with value: r = 0.14. The most affected component by the form was protein (0.28). A slight influence of pregnancy with Rf value = 0.06 arose too. Next evaluated indicator of milk quality was somatic cell count (SSC). The influence of the form, on SSC, was moderately significant (-0.05). The average somatic cell count, during the monitored period, was 287.75 thousand per milliliter. The prolificacy within the herd appears to be inconvenient. Within the population of the herd, the herd showed to be better at pregnancy rate after the first insemination (46.7%), however after all inseminations it proved to be weaker (44%). Subsequently the effect of three factors was evaluated: the day of pregnancy, the day of calving and the age in days at form. For dairy cows, at first lactation, there was found one significant influence the age, with the value of the correlation coefficient 0.23, while the cows at higher lactation showed to be influenced by all three of these factors. The dependence between the pregnancy day and the BCS is expressed as a negative (-0.06) but very weak. Higher absolute value of the correlation coefficient was observed between the age, in days, and BCS (-0.14). BCS value was directly proportional to days, since calving (0.16). This thesis was carried out with the support of the project Gaju 019/2016 / Z Nutrition and breeding technology as a way to animal welfare and the product quality.

The aim of this diploma project is to work out an overviw about temporary cold-blooded horse families bred in Czech republic. Based on breed evidence and information from Approved breed associations the overview is to introduce horse families appearing in cold-blooded breeds (Bohemian-Moravian belgic horse, noric horse and Selezian noric).In individual families all information about their (number of females in the family) are mentioned, according to efficiency tests. Investigation about background of horse breeders helps to gain broader view on cold-blooded horses breeding issue - from the point of viw of motivation, attraction and ambitions of breeders.The source is the serial about breeding codblooded horses written by Jindřiška Svobodová, published in magazine Jezdectví in 2015, entitled: ¨Serial about new generation¨ - which deals with an issue about horses sentenced to scanzens and archives for their redundancy. Results lead to conclusion and recommendation for breed activities of Approved breed association.