Les travaux de cette thèse portent sur la détection et le diagnostic des défauts naissants dans les systèmes d’ingénierie et industriels, par des approches statistiques non-paramétriques. Un défaut naissant est censé provoquer comme tout défaut un changement anormal dans les mesures des variables du système. Ce changement est imperceptible mais aussi imprévisible dû à l’important rapport signal-sur défaut, et le faible rapport défaut-sur-bruit caractérisant le défaut naissant. La détection et l’identification d’un changement général nécessite une approche globale qui prend en compte la totalité de la signature des défauts. Dans ce cadre, la divergence de Kullback-Leibler est proposée comme indicateur général de défauts, sensible aux petites variations anormales cachées dans les variations du bruit. Une approche d’analyse spectrale globale est également proposée pour le diagnostic de défauts ayant une signature fréquentielle. L’application de l’approche statistique globale est illustrée sur deux études différentes. La première concerne la détection et la caractérisation, par courants de Foucault, des fissures dans les structures conductrices. La deuxième application concerne le diagnostic des défauts de roulements dans les machines électriques tournantes. En outre, ce travail traite le problème d’estimation de l’amplitude des défauts naissants. Une analyse théorique menée dans le cadre d’une modélisation par analyse en composantes principales, conduit à un modèle analytique de la divergence ne dépendant que des paramètres du défaut. / This phD dissertation deals with the detection and diagnosis of incipient faults in engineering and industrial systems by non-parametric statistical approaches. An incipient fault is supposed to provoke an abnormal change in the measurements of the system variables. However, this change is imperceptible and also unpredictable due to the large signal-to-fault ratio and the low fault-to-noise ratio characterizing the incipient fault. The detection and identification of a global change require a ’global’ approach that takes into account the total faults signature. In this context, the Kullback-Leibler divergence is considered to be a ’global’ fault indicator, which is recommended sensitive to abnormal small variations hidden in noise. A ’global’ spectral analysis approach is also proposed for the diagnosis of faults with a frequency signature. The ’global’ statistical approach is proved on two application studies. The first one concerns the detection and characterization of minor cracks in conductive structures. The second application concerns the diagnosis of bearing faults in electrical rotating machines. In addition, the fault estimation problem is addressed in this work. A theoretical study is conducted to obtain an analytical model of the KL divergence, from which an estimate of the amplitude of the incipient fault is derived.

La problématique des catastrophes aériennes en Afrique centrale peut être appréhendée sous une approche systémique qui questionne l'efficacité et l'efficience du droit applicable. En effet, régi par des principes et règles, le droit applicable au traitement juridique des catastrophes aériennes pose un questionnement profond de l'ensemble des règles de droit mises en place pour remédier à la grande insécurité aérienne dénoncée par les organisations internationales. C'est ainsi que dans la recherche des sources de droit applicable, il a pu être établi que l'ordonnancement des sources formelles présente une architecture complexe. Concrètement, le droit applicable au traitement juridique des catastrophes aériennes en Afrique centrale est marqué par un pluralisme juridique. La première conséquence est la compétence concurrente et/ou complémentaire de trois règles communautaires à régir les mêmes faits, alors qu'elles sont issues d'ordres juridiques bien distincts. De même, le droit international n'est pas en reste, sans toutefois mettre de côté le niveau divergent des pays d'Afrique centrale en ce qui concerne la ratification des conventions de droit international aérien. Ainsi, dans la mesure où l'ordre juridique interne est seul, il ne peut intervenir pour ordonner l'agencement du droit conventionnel. Par ailleurs, il est ressorti que les multiples sources formelles relevaient du droit commun. En effet, les sources matérielles du traitement juridique des catastrophes aériennes en Afrique centrale sont tantôt celles de l'enquête accident d'aviation civile, tantôt tout simplement celles de droit pénal et de droit civil général. Ce détachement des faits, caractéristique de catastrophes aériennes, est manifeste dans le droit matériel applicable qui ignore la singularité de la catastrophe aérienne d'être un accident collectif. Cette situation qui s'étend à la mise en œuvre du traitement juridique des catastrophes aériennes est marquée par un classique, tant de la titularité de l'action en justice que de celle de la compétence matérielle de la juridiction. Elle appelle à connaître de ce type de contentieux, qui sont pourtant des contentieux sui generis. Si ce régime confirme en tout point le caractère général et abstrait de la règle de droit, il convient de ne pas oublier que le droit est une solution juridique technique qui se doit d'apporter une réponse concrète et satisfaisante à un fait de société surtout lorsque celui-ci perdure. / The problem of air disasters in Central Africa can be apprehended under a systematic approach that questions the efficiency and effectiveness of the applicable law. Indeed, governed by principles and rules, the law applicable to the legal treatment of air disasters raises a profound questioning of all the legal rules put in place to remedy the great air insecurity denounced by international organizations. Thus, in the search for sources of applicable law, it has been established that the scheduling of formal sources presents a complex architecture. In practical terms, the law applicable to the legal treatment of air disasters in Central Africa is marked by legal pluralism. The first consequence is the competing and / or complementary competence of three Community rules to govern the same facts, even though they come from distinct legal orders. Similarly, international law is not left out, however, without setting aside the divergent level of Central African countries in the ratification of conventions of international air law. Thus, insofar as the domestic legal system is alone, it cannot intervene to order the arrangement of the conventional law. Moreover, it emerged that the multiple formal sources fell under common law. Indeed, the material sources of the legal treatment of air disasters in Central Africa are sometimes those of the civil aviation accident investigation, sometimes simply those of criminal law and general civil law. This detachment of facts, characteristic of air disasters, is manifest in the applicable substantive law which ignores the singularity of the air disaster to be a collective accident. This situation which extends to the implementation of the legal treatment of air disasters is marked by a classic, both the ownership of the action in court and the jurisdiction of the jurisdiction. It calls to deal with this type of litigation, which are sui generis litigation. If this diet at any point confirms the general and abstract rule of law character, it should be not to forget that the right is a technical legal solution that needs to provide a concrete and satisfactory to a fact of society especially when response it endures.

This diploma thesis deals with the insolvency administrator. The objects of this work are mainly are the duties that insolvency administrator has to execute during the insolvency proceeding and the necessary requirements for the performance of the insolvency administrator's function. The objective of this work is to clarify the role of insolvency administrator in the particular phases of insolvency proceedings in general terms and the definition of his position and responsibilities in different types of insolvency proceedings. The aim of this work is also to analyze the assumptions that insolvency administrator must meet in order to obtain permission to perform the function of the insolvency administrator.

Esta dissertação apresenta em primeira etapa o trabalho desenvolvido para averiguar a adequação dos modelos reológicos de Mohr-Coulomb e de Endurecimento de solo, para representar as deformações e distorções que ocorrem no sistema soloestrutura, quando do processo de escavação de valas escoradas. Além disto, em uma segunda etapa fazem-se estimativas de previsão de danos causados em edificações, em decorrência das escavações de uma vala escorada da linha 1 do Metropolitano de São Paulo (Metrô S.P.). A primeira e a segunda etapa foram feitas através de 810 simulações numéricas, em elementos finitos utilizando-se o software PLAXIS, associadas a retroanálise por processo direto do módulo de deformabilidade a 50% da tensão de ruptura dos solos utilizando-se para isto o processo direto. Apresenta-se também uma síntese da formação e dos tipos de solos que constituem a bacia sedimentar de São Paulo, onde se localiza a seção experimental nº1 objeto de estudo desta dissertação. Faz-se uma breve revisão bibliográfica a respeito das técnicas de retroanálise. Apresentam-se critérios para escolha de intervalos de parâmetros geotécnicos que representem o sistema solo-estrutura no processo de escavação. Foram feitas análises paramétricas para determinar os parâmetros geotécnicos que mais influenciam o sistema solo-estrutura. Comparam-se os modelos constitutivos de Mohr-Coulomb e de endurecimento na previsão das deformações. Por fim, faz-se a previsão do nível de danos causados pelas escavações da vala, a um edifício hipotético / This dissertation presents, in a first part, the work done to verify how appropriate are the Mohr-Coulomb and hardening soil constitutive models to represent the strains and the distortions associated with escavations of braced wall process. In the second part, estimates are made of the damages in constructions due to the braced excavations of line one of Sao Paulo Subway (Metrô S.P.). In the two phases, 810 numeric simulations were made, in finite elements using the software PLAXIS, associated the back analysis for direct process. It is presented a synthesis of the formation and the types of soils that constitute the basin of the sediments of the city of São Paulo, where is located the section experimental nº1, object of study of this dissertation. It is presented an short bibliographical revision regarding the back analysis techniques. Criteria for choice of intervals of parameters geotechnical that represent the system soil-structure in the excavation process are presented. Parametric analyses are made to determine which the parameters have larger influence in the behavior of the system soil-structure. The behavior of the soil-structure system is simulated using the Mohr-Coulomb and hardening soil constitutive models. The Mohr-Coulomb and hardening soil constitutive models are compared in the forecast of the deformations. Finally, it is made the forecast of the level of damages to a hypothetical building caused by the braced excavations

Submitted by Mariana Dornelles Vargas (marianadv) on 2015-04-24T13:39:08Z No. of bitstreams: 1 leitura_fenomenologica.pdf: 703424 bytes, checksum: cb21d7d26ebfe1ba34e92a360b708342 (MD5) / Made available in DSpace on 2015-04-24T13:39:08Z (GMT). No. of bitstreams: 1 leitura_fenomenologica.pdf: 703424 bytes, checksum: cb21d7d26ebfe1ba34e92a360b708342 (MD5) Previous issue date: 2009-08-28 / Nenhuma / Examina-se, nesta dissertação, a viabilidade da responsabilidade civil no âmbito do direito de família em um diálogo transdisciplinar com a fenomenologia hermenêutica. O tema central deste trabalho é a análise do paradoxo estabelecido entre a perspectiva de indenização de danos decorrentes do término do afeto, no âmbito da relação de família, com fundamento na dignidade da pessoa humana. Na perspectiva da Constituição Federal de 1988, a dignidade da pessoa humana tornou-se fundamento central do direito e de ordem normativa infraconstitucional, mas faz-se necessário dar novo destaque à discussão da pessoa humana, trazendo o enfoque filosófico fenomenológico. A dignidade da pessoa humana se tornou o fundamento para todo e qualquer direito que se queira justificar. Para auxiliar tanto na caracterização quanto na demonstração do objeto em estudo, estuda-se a principal doutrina e a jurisprudência acerca da matéria, notadamente da Argentina e do Brasil, países que apresentam teorias doutrinárias e decisões jurisprudenciais fundamentalmente opostas. A dissertação evidencia, em última análise, que o horizonte fenomenológico do ser humano ainda está distante das decisões judiciais, não raro lastreadas na convicção última de cada julgador. A partir do surgimento dos novos direitos, as transformações sociais impuseram aos operadores e teóricos do direito a necessidade de revisitarem conceitos humano-fundamentais, sobretudo face à complexidade da sociedade moderna. Por esta razão, o presente trabalho situa-se dentro da linha de pesquisa do programa de Pós-Graduação em Direito da Universidade do Vale do Rio dos Sinos, denominada "Sociedade, Novos Direitos e Transnacionalização". / The thesis examines the civil liability on the family law on a transdisciplinar dialogue with heideggerian phenomenology. The central theme of this work is to analyze the paradox between the prospect of liability when the affection within the relationship of family ends, based on human dignity. According to the Federal Constitution of 1988, human dignity became a central foundation of law and normative infra-constitutional order, but it is necessary to give new focus to the discussion of the person bringing the phenomenological approach. Human The dignity has became the reason for any right. In order to help in the as in the demonstration of the object under study, it examines the main doctrine and jurisprudences, especially in Argentina and Brazil, countries with theories and jurisprudential decisions fundamentally opposed. The thesis shows that the phenomenological horizon of the human being has not arrived on trials, often validated by the last conviction of each judge. As the new rights emerge, the social changes imposed on law operators and theoretical a urgent need to revisit human concepts, particularly about the complexity of modern society, which is why this work is situated within the research line of the program of Graduate Studies in Law of the University Vale do Rio dos Sinos, called "Society, New Rights and Transnationalism."

<p>Повредом права конкуренције Европске уније (ЕУ) наноси се, по правилу, штета великом броју лица. Тиме се угрожава остваривање не само општих или јавних интереса, већ се угрожавају и интереси и права појединаца. Међутим, у праву ЕУ је дуго времена након оснивања Европске економске заједнице (ЕЕЗ) основна пажња била посвећена заштити јавних интереса, чему је био подешен и механизам јавно-правне или административне заштите, као и висина и карактер санкција. Из тих разлога су све до последњих деценија и теоријска истраживања углавном била усмерена на анализу дејства јавно-правне примене прописа о конкуренцији на непосредне учеснике на тржишту. Имајући то у виду, предмет овог рада је усмерен на други аспект заштите која се остварује у поступку приватно-правне примене, у коме накнада штете представља грађанско правну санкцију због повреде ЕУ права конкуренције.<br />Тема је подједнако значајна у практичном и у теоријском смислу. У практичном смислу, јер се повредом правила о заштити конкуренције угрожава успостављање и несметано функционисање унутрашњег тржишта, као општег циља оснивања ЕЕЗ/ЕУ. Са аспекта интереса појединаца, њима се онемогућава да користе погодности таквог тржишта на коме би према правилима слободне конкуренције и закона понуде и тражње, остали само најуспешнији, са најбољом робом и услугама. Будући да се не могу спречити повреде конкуренције, у раду су анализиране превентивне и компензаторне мере заштите. У случају повреде јавних интереса, то се остварује високим казнама и заштитним мерама. У случају повреде индивидуалних интереса, оштећеним појединцима је признато право на потпуну накнаду настале штете.<br />У теоријском смислу, питање накнаде штете је у ЕУ крајем деведесетих година прошлог века анализирано и у контексту отклањања демократског дефицита у склопу &quot;конституционалазације и легитимизације&quot; ЕЗ/ЕУ, кад су у центар интересовања постављени интереси појединаца и заштита њихових субјективних права пред националним судовима држава чланица. Разумевање овог процеса подразумева налажење одговора на питање да ли Уговор о функционисању<br />Европске уније (УФЕУ), као врста вишестраног међународног уговора, ствара субјективна права на која се непосредно могу позивати појединци и тражити њихову заштиту пред националним судовима. У питању је дејство одредби чланова 101. и 102. УФЕУ, којима су забрањени рестриктивни споразуми и злоупотреба доминантног положаја учесника на тржишту. Питање је важно јер се забраном закључивања рестриктивних споразума и санкционисањем понашања на тржишту које се сматра злоупотребом доминантног положаја у значајној мери ограничава дејство општепризнатог начела аутономије воље привредних субјеката и начело предузетништва.<br />Како тзв. &quot;неведљива рука тржишта&quot; није у стању да омогући адекватну заштиту и јавноправних и приватноправних интереса, као и да није могуће спречити закључивање рестриктивних споразума, у раду се полази од тезе да држава има тзв. &quot;регулаторну одговорност&quot; да појединцима омоогући остваривање права на накнаду штете, као неотуђиво субјективно право. Признање оваквог субјективног права је образложено садржином и карактером посебних начела права ЕУ, као што је начело директног дејства и начело делотворне примене. Кандидат се залаже да се у складу са начелом делотворне примене у државама чланицама успостави механизам колективне заштите индивидуалних интереса путем репрезентативних тужби по opt-out моделу.<br />У раду су наведена питања анализирана у три дела, који су насловљени као: општа разматрања, садржина и природа права на накнаду штете и поступак остваривања права на накнаду</p> / <p>Povredom prava konkurencije Evropske unije (EU) nanosi se, po pravilu, šteta velikom broju lica. Time se ugrožava ostvarivanje ne samo opštih ili javnih interesa, već se ugrožavaju i interesi i prava pojedinaca. Međutim, u pravu EU je dugo vremena nakon osnivanja Evropske ekonomske zajednice (EEZ) osnovna pažnja bila posvećena zaštiti javnih interesa, čemu je bio podešen i mehanizam javno-pravne ili administrativne zaštite, kao i visina i karakter sankcija. Iz tih razloga su sve do poslednjih decenija i teorijska istraživanja uglavnom bila usmerena na analizu dejstva javno-pravne primene propisa o konkurenciji na neposredne učesnike na tržištu. Imajući to u vidu, predmet ovog rada je usmeren na drugi aspekt zaštite koja se ostvaruje u postupku privatno-pravne primene, u kome naknada štete predstavlja građansko pravnu sankciju zbog povrede EU prava konkurencije.<br />Tema je podjednako značajna u praktičnom i u teorijskom smislu. U praktičnom smislu, jer se povredom pravila o zaštiti konkurencije ugrožava uspostavljanje i nesmetano funkcionisanje unutrašnjeg tržišta, kao opšteg cilja osnivanja EEZ/EU. Sa aspekta interesa pojedinaca, njima se onemogućava da koriste pogodnosti takvog tržišta na kome bi prema pravilima slobodne konkurencije i zakona ponude i tražnje, ostali samo najuspešniji, sa najboljom robom i uslugama. Budući da se ne mogu sprečiti povrede konkurencije, u radu su analizirane preventivne i kompenzatorne mere zaštite. U slučaju povrede javnih interesa, to se ostvaruje visokim kaznama i zaštitnim merama. U slučaju povrede individualnih interesa, oštećenim pojedincima je priznato pravo na potpunu naknadu nastale štete.<br />U teorijskom smislu, pitanje naknade štete je u EU krajem devedesetih godina prošlog veka analizirano i u kontekstu otklanjanja demokratskog deficita u sklopu &quot;konstitucionalazacije i legitimizacije&quot; EZ/EU, kad su u centar interesovanja postavljeni interesi pojedinaca i zaštita njihovih subjektivnih prava pred nacionalnim sudovima država članica. Razumevanje ovog procesa podrazumeva nalaženje odgovora na pitanje da li Ugovor o funkcionisanju<br />Evropske unije (UFEU), kao vrsta višestranog međunarodnog ugovora, stvara subjektivna prava na koja se neposredno mogu pozivati pojedinci i tražiti njihovu zaštitu pred nacionalnim sudovima. U pitanju je dejstvo odredbi članova 101. i 102. UFEU, kojima su zabranjeni restriktivni sporazumi i zloupotreba dominantnog položaja učesnika na tržištu. Pitanje je važno jer se zabranom zaključivanja restriktivnih sporazuma i sankcionisanjem ponašanja na tržištu koje se smatra zloupotrebom dominantnog položaja u značajnoj meri ograničava dejstvo opštepriznatog načela autonomije volje privrednih subjekata i načelo preduzetništva.<br />Kako tzv. &quot;nevedljiva ruka tržišta&quot; nije u stanju da omogući adekvatnu zaštitu i javnopravnih i privatnopravnih interesa, kao i da nije moguće sprečiti zaključivanje restriktivnih sporazuma, u radu se polazi od teze da država ima tzv. &quot;regulatornu odgovornost&quot; da pojedincima omoogući ostvarivanje prava na naknadu štete, kao neotuđivo subjektivno pravo. Priznanje ovakvog subjektivnog prava je obrazloženo sadržinom i karakterom posebnih načela prava EU, kao što je načelo direktnog dejstva i načelo delotvorne primene. Kandidat se zalaže da se u skladu sa načelom delotvorne primene u državama članicama uspostavi mehanizam kolektivne zaštite individualnih interesa putem reprezentativnih tužbi po opt-out modelu.<br />U radu su navedena pitanja analizirana u tri dela, koji su naslovljeni kao: opšta razmatranja, sadržina i priroda prava na naknadu štete i postupak ostvarivanja prava na naknadu</p> / <p>Violation of the EU competition law makes damage to a large number of persons: direct competitors, customers and end-users of services. This endangers the implementation of not only public interests, but also the interests and subjective rights of individuals. However, long after the founding of the EEC, community law focused on the protection of public interests, to which the mechanism of public law or administrative enforcement, as well as the amount and character of the sanction, was adjusted. For these reasons, until the last decades, theoretical research mainly focused on analysing the effects of public law enforcement on the immediate participants in the market as well. Therefore, the subject of this paper is focused on another aspect, the so-called private enforcement, whose part is the right to compensation of damages as a civil law sanction for violation of the EU competition law.<br />The subject is equally important in practical and theoretical sense. Practical importance is reflected in the fact that by violating the rules on the protection of competition, damage is caused to a large number of participants on the market, thus jeopardizing the achievement of both general and individual goals. As a general economic objective in the EC/EU Treaties, proclaim the establishment of an internal market. On the other hand, individuals want to make full use of the benefits of such a market in which, according to the rules of free competition and rules of supply and demand, only the most successful, with the best goods and services, would remain. Practice, however, has shown that market participants often violate competition rules and thus the question is raised of how to eliminate the harmful consequences of the violation in the best possible way. When it comes to public interests, this is achieved through high penalties and protective measures, which are decided by the European Commission and national regulatory bodies. In the event of a violation of individual interests, this can be achieved by recognition of the full compensation of damages.<br />The issue of compensation for damages in the EU was set in parallel with the elimination of the democratic deficit and in the context of the &quot;institutionalization and legitimization&quot; of the EC/EU in the late 1990s, when the interests of individuals and the protection of their subjective rights were placed before the national courts of the member states.<br />Understanding of this process implies finding the answer to the question of whether the Treaty on the Functioning of the European Union (TFEU), as a type of multilateral international treaty, creates subjective rights to which individuals can directly refer and request their protection before national courts.<br />These are the provisions of Articles 101 and 102 of the TFEU that regulate the issue of restrictive agreements and the dominant position of participants in the market by banning and automatically considering null and void the first ones, while the abuse of a dominant position is considered prohibited and punishable. The prohibition of the conclusion of restrictive agreements and the sanctioning of market behaviour which is considered to be the abuse of a dominant position is significantly limited to the generally accepted principle of autonomy of the will of business entities and the raight of establishement.<br />Since it is impossible to prevent the conclusion of restrictive (cartel) agreements, the solution was found in the recognition of the right to compensation as one of the inalienable subjective rights belonging to individuals. The recognition of this subjective right, due to the particular and dual nature of EU law, as supranational and national, is explained by the new principles of EU law, such as the principle of direct effect and the principle of effectiveness. The principle of effectiveness will also serve as a theoretical basis for establishing the principles of procedural autonomy, as a legal framework for judicial protection.<br />The author analyses the subject issues in three parts, which are addressed as: general considerations, content and nature of the right to compensation of damage and the procedure for enforcement the right to compensation. The paper starts from the thesis that the public law and private law enforcement of the EU competition law are complementary and that the compensation of damages should be implemented not only in individual lawsuits, but also by introducing collective redress mechanism for the protection of collective interests.</p>

La pollution atmosphérique constitue de nos jours un enjeu sociétal majeur, tant pour ses conséquences néfastes sur la santé humaine que sur l'environnement. L'objectif général de ce présent travail était de déterminer le niveau de la pollution atmosphérique dakaroise, extérieure et intérieure, et d'évaluer son impact sanitaire au sein de la population urbaine. Les particules fines (PM₂.₅) et une fraction plus grossière (PM>₂.₅) échantillonnées sur un site urbain de Dakar (HLM), caractérisées par de fortes émissions du trafic routier, ont été comparées aux particules échantillonnées dans une zone rurale, Toubab Dialaw, située à environ 40 kms de Dakar. Les caractéristiques physicochimiques des échantillons ont révélé que les particules différaient par leurs propriétés physiques (surface) et chimiques (en termes de CHN, métaux, ions, paraffines, COV et HAP) de 65 à 75 % plus élevées dans les échantillons urbains. Selon les différentes sources et les différentes propriétés physiques et chimiques, la réponse inflammatoire (TNF-α, IL-1β, IL-6, IL-8) et les dommages oxydatifs (protéines carbonylées totales et 8-OHdG) se sont révélés plus importants dans les cellules bronchiques BEAS-2B exposées aux particules urbaines. La campagne de mesure de la qualité de l'air a montré que l'intérieur du bus était plus pollué en PM₁₀, CO, CO₂ et NO que le marché et les sites intérieurs urbains et ruraux. La comparaison de la qualité de l'air entre les sites intérieurs et extérieurs a révélé que, particulièrement dans les zones urbaines, la qualité de l'air intérieur pouvait être moins bonne que celle des sites extérieurs correspondants. Le suivi de l'exposition individuelle aux polluants a montré que les ménagères de la zone urbaine et rurale étaient plus exposées que les professionnels à la pollution de l'air, confirmant les observations précédentes d'un niveau d'exposition potentiellement plus élevé des polluants dans l'environnement intérieur. Cependant, il convient de tenir compte du niveau d'exposition des commerçants et des conducteurs d'autobus résultant de l'échappement du trafic avec des véhicules souvent anciens, mal entretenus et non contrôlés, en raison des niveaux élevés de polluants gazeux rapportés ici. / Air pollution is nowadays a major societal challenge, as much for its harmful consequences on human health as on the environment. The general objective of this work was to determine the level of Dakar air pollution, outdoor and indoor, and to assess its health impact in the urban population. Fine particles (PM₂.₅) and a coarser fraction (PM>₂.₅) sampled at an urban site in Dakar (HLM), characterized by high road traffic emissions, were compared with particles sampled at a rural area, Toubab Dialaw located about 40 km from Dakar. The physicochemical characteristics of samples revealed that PMs differ for their physical (surface area) and chemical properties (in terms of CHN, metals, ions, paraffins, VOCs and PAHs) that were 65 to 75 % higher in urban samples. In accordance with the different sources and different physical and chemical properties, the inflammatory response (TNF-α, IL-1β, IL-6, IL-8) and the oxidative damage (total carbonylated proteins and 8-OHdG) were found higher in bronchial BEAS-2B cells exposed to urban PMs. The air quality measurement campaign showed that the bus interior was more polluted with PM₁₀, CO, CO₂ and NO than the market and the urban and rural indoor sites. The comparison of air quality between indoor and outdoor sites revealed that, particularly in urban areas, indoor air quality may be worse than the corresponding outdoor one. Monitoring individual exposure to pollutants showed that housewives in urban and rural sites were more exposed than professionals to air pollution, confirming previous observations of potential higher individual exposure level to pollutants in indoor environment. However, exposure level of traders and bus drivers that would result from the exhaust of traffic with often old, poorly maintained and uncontrolled vehicles has to be taken into consideration due the higher levels of gaseous pollutants here reported.

Les microfaisceaux d’ions ont, au cours de ces dernières décennies, montré leur efficacité dansl’étude des effets des rayonnements ionisants sur le vivant notamment concernant les effets des faiblesdoses ou l’étude de l’effet de proximité. Le CENBG dispose depuis 2003 d’un dispositif permettant la micro-irradiation ciblée d’échantillons biologiques vivants. Les applications des microfaisceaux dans ce domainese sont récemment diversifiées et des études plus fines sur les mécanismes de réparation desdommages ADN radio-induits aux échelles cellulaire et multicellulaire sont devenues possibles via lesévolutions en imagerie par fluorescence et en biologie cellulaire. Ces approches ont nécessité une évolutionimportante de l'instrumentation de la ligne de micro-irradiation du CENBG qui a été entièrementredessinée et reconstruite dans un souci d’optimisation d’apport de nouvelles fonctionnalités. Les objectifsde mes travaux ont été i) la mise en service du dispositif, ii) la caractérisation des performances dusystème, iii) la mise en place de protocoles pour l’irradiation ciblée à dose contrôlée aux échelles cellulaireet multicellulaire, in vitro et in vivo, et le suivi en ligne des conséquences précoces de cette irradiation,iv) la modélisation des irradiations afin d’interpréter les observables biologiques au regard des donnéesphysiques calculées.Ces travaux ont permis i) de caractériser les performances du dispositif : une taille de faisceau d’environ2 μm sur cible et une précision de tir de ± 2 μm, de développer des systèmes de détection d’ions pour uncontrôle absolu de la dose délivrée, ii) d’induire des dommages ADN fortement localisés in vitro, et devisualiser en ligne le recrutement de protéines impliquées dans la réparation de ces dommages,iii) d’appliquer ces protocoles pour générer des dommages ADN in vivo au sein d’un organisme multicellulaireau stade embryonnaire, Caenorhabditis elegans.Ces résultats ouvrent la voie vers des expériences plus fines sur la ligne de micro-irradiation ciblée duCENBG pour étudier les effets de l’interaction des rayonnements ionisants avec le vivant, aux échellescellulaire et multicellulaire, in vitro et in vivo. / The main goal of radiobiology is to understand the effects of ionizing radiations on the living.These past decades, ion microbeams have shown to be important tools to study for example the effects oflow dose exposure, or the bystander effect. Since 2003, the CENBG has been equipped with a system toperform targeted micro-irradiation of living samples. Recently, microbeams applications on this subjecthave diversified and the study of DNA repair mechanisms at the cellular and multicellular scales, in vitroand in vivo, has become possible thanks to important evolutions of fluorescence imaging techniques andcellular biology. To take into account these new approaches, the CENBG micro-irradiation beamline hasbeen entirely redesigned and rebuilt to implement new features and to improve the existing ones. My PhDobjectives were i) commissioning the facility, ii) characterizing the system on track etch detectors, and onliving samples, iii) implementing protocols to perform targeted irradiations of living samples with a controlleddelivered dose, at the cellular and multicellular scales, and to visualize the early consequencesonline, iv) modelling these irradiations to explain the biological results using the calculated physical data.The work of these past years has allowed us i) to measure the performances of our system: a beam spotsize of about 2 μm and a targeting accuracy of ± 2 μm, and to develop ion detection systems for an absolutedelivered dose control, ii) to create highly localized radiation-induced DNA damages and to see onlinethe recruitment of DNA repair proteins, iii) to apply these protocols to generate radiation-induced DNAdamages in vivo inside a multicellular organism at the embryonic stage: Caenorhabditis elegans.These results have opened up many perspectives on the study of the interaction between ionizing radiationsand the living, at the cellular and multicellular scales, in vitro and in vivo.

<p>Quantum chemical methods have been used to provide a better understanding of the photochemistry of astaxanthin and phytochromobilin; the photoenzymic repair of UV-light induced DNA damages; and the formation of lignin. </p><p>The carotenoid astaxanthin (AXT) is responsible for the colouration of lobster shell. In solution, the electronic absorption spectra of AXT peak in the 470-490 nm region, corresponding to an orange-red colouration. Upon binding to the lobster-shell protein-complex α-crustacyanin, the absorption maximum is shifted to 632 nm, yielding a slate-blue colouration. Herein, the structural origin of this bathochromic shift is investigated on the basis of recent experimental work.</p><p>The tetrapyrrole phytochromobilin (PΦB) underlies the photoactivation of the plant photoreceptor phytochrome. Upon absorption of 660-nm light, PΦB isomerizes from a C15-<i>Z,syn</i> configuration (in the inactive form of the protein) to C15-<i>E,anti</i> (in the active form). In this work, a reaction mechanism for this isomerization is proposed. </p><p>DNA photolyases are enzymes that repair DNA damages resulting from far-UV-light induced [2+2] cycloaddition reactions involving pyrimidine nucleobases. The catalytic activity of these enzymes is initiated by near-UV and visible light, and is governed by electron transfer processes between a catalytic cofactor of the enzyme and the DNA lesions. Herein, an explanation for the experimental observation that the repair of cyclobutane pyrimidine dimers (CPD) – the major type of lesion – proceeds by electron transfer from the enzyme to the dimer is presented. Furthermore, the formation of CPD is studied.</p><p>Lignin is formed by dehydrogenative polymerization of hydroxycinnamyl alcohols. A detailed understanding of the polymerization mechanism and the factors controlling the outcome of the polymerization is, however, largely missing. Quantum chemical calculations on the initial dimerization step have been performed in order to gain some insight into these issues.</p>

Quantum chemical methods have been used to provide a better understanding of the photochemistry of astaxanthin and phytochromobilin; the photoenzymic repair of UV-light induced DNA damages; and the formation of lignin. The carotenoid astaxanthin (AXT) is responsible for the colouration of lobster shell. In solution, the electronic absorption spectra of AXT peak in the 470-490 nm region, corresponding to an orange-red colouration. Upon binding to the lobster-shell protein-complex α-crustacyanin, the absorption maximum is shifted to 632 nm, yielding a slate-blue colouration. Herein, the structural origin of this bathochromic shift is investigated on the basis of recent experimental work. The tetrapyrrole phytochromobilin (PΦB) underlies the photoactivation of the plant photoreceptor phytochrome. Upon absorption of 660-nm light, PΦB isomerizes from a C15-Z,syn configuration (in the inactive form of the protein) to C15-E,anti (in the active form). In this work, a reaction mechanism for this isomerization is proposed. DNA photolyases are enzymes that repair DNA damages resulting from far-UV-light induced [2+2] cycloaddition reactions involving pyrimidine nucleobases. The catalytic activity of these enzymes is initiated by near-UV and visible light, and is governed by electron transfer processes between a catalytic cofactor of the enzyme and the DNA lesions. Herein, an explanation for the experimental observation that the repair of cyclobutane pyrimidine dimers (CPD) – the major type of lesion – proceeds by electron transfer from the enzyme to the dimer is presented. Furthermore, the formation of CPD is studied. Lignin is formed by dehydrogenative polymerization of hydroxycinnamyl alcohols. A detailed understanding of the polymerization mechanism and the factors controlling the outcome of the polymerization is, however, largely missing. Quantum chemical calculations on the initial dimerization step have been performed in order to gain some insight into these issues.