Made available in DSpace on 2014-12-17T14:55:41Z (GMT). No. of bitstreams: 1 ViniciusSVS.pdf: 2441901 bytes, checksum: 1246fceb24d0749c8833cd005b384269 (MD5) Previous issue date: 2010-05-07 / The incorporate of industrial automation in the medical are requires mechanisms to safety and efficient establishment of communication between biomedical devices. One solution to this problem is the MP-HA (Multicycles Protocol to Hospital Automation) that down a segmented network by beds coordinated by an element called Service Provider. The goal of this work is to model this Service Provider and to do performance analysis of the activities executed by in establishment and maintenance of hospital networks / Com a incorpora??o de conceitos da automa??o industrial na ?rea m?dica, surge a necessidade de estabelecer mecanismos que permitam a comunica??o entre os dispositivos biom?dicos de maneira segura e eficiente. Dentre as solu??es para esse problema tem-se o PM-AH (Protocolo Multiciclos para Automa??o Hospitalar) que estabelece uma rede segmentada por leitos e coordenada por um elemento chamado Provedor de Servi?os. O objetivo desse trabalho ? modelar esse Provedor de Servi?os e efetuar an?lises de desempenho sobre as tarefas realizadas pelo mesmo no estabelecimento e manuten??o da rede hospitalar

Se estudió las emisiones de material particulado (PM), vapores y gases de chimenea del incinerador del Instituto Nacional de Enfermedades Neoplásicas y se registró la micrometeorolo' gía. Los gases de chimenea fueron recolectados con un muestreador isocinético; los sólidos totales suspendidos, por muestreo pasivo. Los análisis muestran elevados niveles de PM 10, que contiene Pb, Al, Zn y Ca, así como altas concentraciones de benceno y naftaleno en el gas de chimenea. Se observó que la dispersión del PM tiene escala local. / Toxic substances in gaseous emissions from hospital incineratorsThe Nationallnstitute for Neoplastic Diseases incinerator was studied: emissions were sampled and analyzed for particulate matter (PM), stack gases and vapors and the micrometeorology was also registered. Gases were collected with an isokinetic sampler, total suspended solids by passive sampling. Results show high levels of PM10, which contains Pb, Al, Zn and Ca; high levels of benzene and naphthalene in the gas fireplace. Local dispersion of PM was observed,

La polyprotéine de structure Gag du VIH-1 est responsable de l’assemblage des particules virales dans les cellules infectées. Au niveau moléculaire, cette protéine s’oligomérise en formant des complexes Gag-Gag autour de deux plates-formes moléculaires, d'une part l'ARN génomique via son domaine NCp7 (NucleoCapsid protein 7) et d'autre part, la membrane plasmique via son domaine MA (Matrice). De plus, lors du trafic de Gag dans la cellule, Gag détourne les protéines ESCRT comme TSG101 et ALIX de la machinerie cellulaire afin de bourgeonner et d’être libérées dans le milieu extracellulaire. Dans cette thèse, nous avons étudié le rôle du domaine NCp7 seul ou au sein de Gag (GagNC) dans les interactions Gag-Gag et Gag-TSG101 en utilisant des approches biochimiques et de la microscopie de fluorescence quantitative. Les résultats ont montré que l'absence du domaine NCp7 affecte l’oligomerisation de Gag qui s’accumule alors dans le cytoplasme sous forme d’agrégats de taille importante. Par ailleurs, le trafic intracellulaire de Gag est affecté par les mutations dans le domaine GagNC avec une augmentation importante de temps nécessaire à Gag pour arriver à la membrane plasmique. Enfin, nous avons montré que GagNC i) renforce l’interaction entre le domaine p6 de Gag et TSG101 et ii) par sa fonction dans le trafic de Gag, est responsable de la localisation de TSG101 à la PM. Sur la base de ces résultats, des études sont maintenant en cours pour développer des tests afin d’identifier des molécules possédant un potentiel anti virale. / The Gag structural polyprotein of HIV-1 orchestrates viral particle assembly in producer cells, in a process that requires two platforms, the genomic RNA on the one hand and a membrane with a lipid bilayer, on the other. During its transportation from translating ribosomes to plasma membrane, Gag hijacks cellular proteins of the cytoskeleton and the ESCRT proteins like TSG101, Alix, etc., to egress viral particles. However, a number of questions remain to be answered before they are clearly apprehended. In this thesis, , we studied the role of the NC domain alone or as part of Gag (GagNC) in Gag-Gag and Gag-TSG101 interactions, which are essential for the assembly and budding of HIV-1 particles using quantitative fluorescent microscopy and biochemical approach. Results, showed that the absence of NC domain lead to (1) an accumulation of Gag as large aggregates that are dispersed in the cytoplasm, (2) a decrease of Gag-Gag condensation and (3) a delay for Gag-Gag complexes in reaching the PM, (4) improved interaction between Gag and TSG101, and (5) by its virtue in Gag trafficking docks TSG101 to the PM. This regulatory effect of NCp7 domain in either TSG101 or Gag or both protein- regulated pathways during virus budding can be exploited to develop inhibitors targeting HIV-1.

In the present study, compounds derived from β-lapachone were electrochemically studied in protic (phosphate and acetate buffer) and aprotic (DMF + TBAP) media, using glassy carbon as working electrode. The electroreducedintermediatesof the quinones reacted with oxygen, indicative of the generation of reactive oxygen species. The interaction of the quinones β-lapachone, α-lapachone and nor-β-lapachone with DNA, was investigated using the technique of quartz crystal microbalance.To increase the solubility of the quinones, in order to allow in vivo studies, the formation of inclusion complexes with β-cyclodextrin was evaluated. Positive results were obtained, leading to a viable formulation alternative for further biological studies with the compounds. β-lapachone was studied spectroelectrochemically through the techniques of Polarization Modulation Infrared Reflection Absorption Spectroscopy + Cyclic Voltammetry to confirm the formation of the hydrogen bondfrom the electroreduced β-lapachone and the secondary hydroxy group of β-cyclodextrin. The inclusion complex betweenmangiferin, an antioxidant polyphenoland β-cyclodextrin, was confirmed using the techniques of Fourier Transform Infrared Spectroscopy, Hydrogen Nuclear Magnetic Resonance, Differential Scanning Calorimetry and Polarization Modulation Infrared Reflection Absorption Spectroscopy. Also, the increase of the antioxidant activity of mangiferin was demonstrated when in presence of β-cyclodextrin, by means of radical scavenging activity toward 2,2’-diphenyl-1-picrylhydrazyl radical (RSA-DPPH ), with additional analysis of solvent effects, being demonstrated that below 50% of organic solvent, it is impossible to work with this method. The assay of the capacity of oxygen radical absorbance capacity-fluorescein (ORAC-FL) was also used, showing that the complex mangiferin: β-cyclodextrin is fifteenfold more potent than the standard molecule,Trolox. Using Electrophoresis and Differential Pulse Voltammetry, it was possible to emphasize that the method used by our research group for the ssDNA production (treatment acid-base with heating) is useful, simple, cheap and completely reproducible, being this, one of the methods used to show the interaction between DNA and free or complexed thymol with cyclodextrin. This interaction was still evaluated using dsDNA biosensor through Differential Pulse Voltammetry, Electrochemistry Quartz Crystal Microbalance and Scanning Electrochemistry Microscopy. / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Neste trabalho, derivados da nor-β-lapachona e nor-α-lapachona, foram estudados eletroquimicamente nos meios prótico (tampão fosfato e acetato) e aprótico (DMF + TBAP), utilizando carbono vítreo como eletrodo de trabalho.Os intermediáriosda eletro-redução das quinonas reagiram com oxigênio, indicativo da geração de Espécies Reativas de Oxigênio. A interação das quinonas β-lapachona, α-lapachona e nor-β-lapachona com DNAfoi investigada,utilizando-sea técnica de microbalança de cristal de quartzo. Com o objetivo de aumentar a solubilidade das quinonas, para posteriores estudos in vivo, foi avaliada a formação de complexos de inclusão com β-ciclodextrina, sendo a β-lapachona estudada espectroeletroquimicamente através das técnicas de Espectroscopia de Reflexão-Absorção no Infravermelho com Polarização Modulada + Voltametria Cíclica para confirmar a formação da ligação hidrogênio entre a hidroquinona gerada a partir da β-lapachonae as hidroxilas secundárias da β-ciclodextrina. A formação do complexo de inclusão entre a mangiferina, um composto antioxidante polifenólico, e a β-ciclodextrina foi confirmado utilizando-se as técnicas de Infravermelho com transformada de Fourier, Ressonância Magnética Nuclear de Hidrogênio, Calorimetria Diferencial e Espectroscopia de Reflexão-Absorção no Infravermelho com Polarização Modulada. Foi,também, demonstrado o aumento da atividade antioxidante da xantona supracitada quando em presença de β-ciclodextrina através da medida da atividade sequestradora de radicais frente ao radical 2,2’-difenil-1-picrilidrazil (RSA-DPPH ),com análise adicional do efeito do solvente sendo assim demonstrado que abaixo de 50% de solvente orgânico, é impossível trabalhar com este método. O ensaio da capacidade de absorbância do radical oxigênio-fluoresceína (ORAC-FL) também foi utilizado, mostrando que o complexo mangiferina:β-ciclodextrina é quinze vezes mais potente que a molécula padrão trolox. Com a utilização de ensaios de Eletroforese e Voltametria de Pulso Diferencial, foi possível enfatizar que o método usado por nosso grupo de pesquisa para a produção de ssDNA (tratamento ácido-base com aquecimento) é útil, simples, barato e completamente reprodutível,sendo este um dos métodos utilizados para mostrar a interação entre DNA e timol livre e complexado com ciclodextrina. Esta interação foi ainda avaliada usando biossensor de dsDNA por Voltametria de Pulso Diferencial, Microbalança Eletroquímica de Cristal de Quartzo e Microscopia Eletroquímica de Varredura.

The master thesis deals with the construction and design of a high-speed synchronous machine with an outer rotor. The thesis is organised into three main chapters. The first chapter deals with machine design theory as well as mechanical limits, which are significant for high speed machines. This chapter also provides examples of applications of these electrical machines. In the second chapter, the initial design of the machine is calculated analytically. This machine design is optimised and the third chapter of this work shows the results of simulations using the finite elements method, including the evaluation of these results.

Táto práca sa zaoberá s modelovaním synchronného stroja s permanentnými magnetmi pomocou metody konečných prvkov. K modelovaniu sme si zvolili 3 fázový synchronný stroj s permanentnými magnetmi na povrchu rotora z firmy VUES. Najprv vypočítame parametre stroja a potom spravíme statický model pomocou programu FEMM a Matlab a budeme hladať možné riešenia na 2D dynamický model. Pomocou programu Ansys Workbench spravíme 3D model tohoto stroja budeme a porovnávať výsledky simulácie.

<div>Radio Frequency Power Amplifiers (PA) are essential components of wireless systems and nonlinear in a permanent way. So, high efficiency and linearity at a time are imperative for power amplifiers. However, it is hard to obtain because high efficiency Power Amplifiers are nonlinear and linear Power Amplifiers have poor efficiency. To meet both linearity and efficiency, the linearization techniques such as Digital Predistortion (DPD) has arrested the most attention in industrial and academic sectors due to provide a compromising data between efficiency and linearity. This thesis proposed on digital predistortion techniques to control nonlinear distortion in radio frequency transmitters. </div><div>By using predistortion technique, both linearity and efficiency can obtain. In this thesis a new generic Saleh model for use in memoryless nonlinear power amplifier (PA) behavioral modelling is used. The results are obtained by simulations through MATLAB and experiments. We explore the baseband 13.56 MHz Power Amplifier input and output relationships and reveal that they apparent differently when the Power Amplifier shows long-term, short-term or memory less effects. We derive a SIMULINK based static DPD design depend on a memory polynomial. A polynomial improves both the non-linearity and memory effects in the Power Amplifier. As PA characteristics differs from time to time and operating conditions, we developed a model to calculate the effectiveness of DPD. We extended our static DPD design model into an adaptive DPD test bench using Indirect Learning Architecture (ILA) to implement adaptive DPD which composed of DPD subsystem and DPD coefficient calculation. By this technique, the output of PA achieves linear, amplitude and phase distortions are eliminated, and spectral regrowth is prevented. </div><div>The advanced linearity performance executed through the strategies and methods evolved on this thesis can allow a higher usage of the capability overall performance of existing and emerging exceptionally performance PAs, and therefore an anticipated to have an effect in future wireless communication systems. </div>

Apis mellifera Linnaeus (1758) è un insetto eusociale conosciuto in tutto il mondo sia per la produzione di miele sia per il suo ruolo di impollinatore, uno dei servizi ecosistemici fondamentali per la biodiversità del pianeta. Durante la sua attività di foraggiamento, l’ape è esposta agli inquinanti ambientali tra cui il particolato atmosferico aerodisperso (PM). Il particolato atmosferico può depositarsi sul corpo dell’insetto e infine contaminare anche i prodotti apistici come polline e miele. Il PM può avere diverse dimensioni (es. PM10, PM2.5, PM0.1), composizione chimica, morfologia e fonti di emissione (naturale o antropica). Nel presente elaborato di tesi, tecniche di microscopia elettronica a scansione (SEM-EDX) sono state utilizzate per caratterizzare la contaminazione da PM di origine antropica del corpo dell’ape e dei suoi prodotti (Capitolo 2 e Capitolo 3) e analisi molecolari per studiare gli eventuali effetti sub-letali sul microbiota intestinale di api esposte ai PM per via orale (Capitolo 4). / Apis mellifera Linnaeus (1758) order Hymenoptera family Apidae, is a eusocial insect widely known for its role in pollination, a fundamental ecosystem service for plant biodiversity and ultimately for the planet. During flight and foraging activity, the honey bee can collect airborne particulate matter (PM) on their own body, especially on the forewings, and can also contaminate bee products as pollen and honey. Particulate matter can originate from natural or anthropic sources, and is characterised by size (e.g., PM10, PM2.5, PM0.1), chemical composition, and morphology. In this thesis, honey bee, pollen and honey were used as bioindicator of PM – from coarse to ultrafine – in industrial areas of the Po Valley, Italy (Chapter 2 and Chapter 3). The (sub-lethal) effects of Titanium dioxide – a widespread airborne PM1 pollutant – on the honey bee through oral exposure was then investigated (Chapter 4). The technique used to analyse the PM contaminating bees and bee products is the scanning electron microscopy (SEM) coupled with X-ray spectrometer (EDX). EDX spectra allowed us to obtain chemical information from specimens, while backscattered-electron (BSE) imaging and elemental mapping provided both compositional and topographic information of PM.

På grund av digitala teknologins framväxt står organisationer inför utmaningar där de behövergenomgå digitala transformationer för att möta omgivningens nya krav och behov. För attlyckas i den digitala transformationen behöver organisationer arbeta aktivt med att styraorganisationens digitala resurser och se till att IT och verksamhet samverkar. Rollen somförvaltningsledare återfinns inom ramverket pm³, vilket är en förvaltningsstyrningsmodell somappliceras för att på ett strukturerat sätt kunna prioritera mellan förvaltningens uppdrag, skapaen samverkan mellan IT och verksamhet samt ge en helhetsbild över de digitala resurser somfinns inom organisationen. Syftet med studien är att undersöka och skapa förståelse för rollensom förvaltningsledare i praktiken i en kontext som karaktäriseras av förändring, digitaltransformation samt IT-styrning. Vilket görs genom att studera förvaltningsledarrollen i enmyndighet i Sverige vilken genomgår en digital transformation. Den empiri som studiengrundar sig i är interna dokument, observationer och intervjuer för att ge en rättvis bild av denkontext som studerats. Studiens resultat visar att förvaltningsledarna har en central roll inomden digitala transformationen eftersom de både leder och driver den framåt. De förutsättningarsom har visat sig nödvändiga för att förvaltningsledare ska vara framgångsrika i sin roll ärbland annat: att de har förtroende och stöd; att de har en gemensam vision; att de har deninformation som behövs för att kunna utföra sitt arbete; att samarbetet inom förvaltningen samtäven mellan förvaltningen och övriga delar av organisationen fungerar; att de har förståelse förderas roll som ledande i den digitala transformationen; att de har en organisationsstruktur ochorganisationskultur som främjar arbetet med förvaltning och den digitala transformationen.

In order to improve the air quality in Uppsala, a proposition to introduce one of two new emission zones (EZ), starting in the year 2020, has been proposed. In what is called Environment Zone Class 2 (EZ2), only cars that meet emission class Euro 5 and higher are allowed and in Environment Zone Class 3 (EZ3), only electric, fuel cell and gas vehicles are allowed. The purpose of this thesis is to examine how EZ: s would affect the air quality, regarding nitrogen oxides (NOx) and particles (PMx), within the zone of the city of Uppsala. Using the traffic simulation software PTV Vissim and the emissions modeling software EnViver, four scenarios have been created, two representing today's fleet of vehicles and two examining a modified fleet. Scenario 1 examines an exclusion of all non EZ2 vehicles (Euro 4 and lower) within the zone and scenario 2 examines an EZ2 solely on the road Kungsgatan. Scenario 3 and 4 examine an EZ2 and EZ3 where all cars that do not currently meet the requirements for each EZ are replaced with ones that do. The results indicate that all proposals, except scenario 2, lead to a reduction of NOx and PM2 within the zone. Scenario 1 shows a decrease by 51% for NOx and 57% for PM10, scenario 3 shows a decrease by 17% and 24% respectively and scenario 4 shows a decrease by 66% and 43% respectively. For scenario 2 the emissions show an increase by 10% and 7% each within the zone.