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The Global ETD Search service is a free service for researchers
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Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 131 to 139 of 139 (0.05 seconds)Spelling suggestions: "subject:"disision cience"" "subject:"disision cscience""
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Investigations on the Reptilian Spectaclevan Doorn, Kevin January 2012 (has links)
The eyes of snakes and most geckos, as well as a number of other disparate squamate taxa, are shielded beneath a layer of transparent integument referred to as the “reptilian spectacle.” Derived from the embryonic fusion of palpebral tissues, the spectacle contains a number of specializations of the skin to benefit vision while still allowing it to function as the primary barrier to the environment. For example, in nearly all species that possess it, it is markedly thinned compared to the surrounding integument and its keratinized scale is optically transparent. While the spectacle may thus seem ideally adapted to vision in allowing the eyes to be always unoccluded, it does have a few drawbacks. One such drawback is its vascularity, the implications of which are still not fully understood, but are explored herein. As no recent synthesis exists of the body of knowledge on reptilian spectacles, the first chapter of this thesis consists of a review of spectacle anatomy, physiology, adaptive significance and evolution to help put into context the following chapters that present original research. The second chapter describes the dynamics of blood flow through the spectacle vasculature of colubrid snakes, demonstrating three main points: (1) that the spectacle vasculature exhibits cycles of regular dilation and constriction, (2) that the visual perception of a threat induces vasoconstriction of its vessels, and (3) that spectacle vessels remain dilated throughout the renewal phase. The implications of these points are discussed. The third chapter describes the spectral transmittance of the shed spectacle scale, the only keratinized structure in the animal kingdom to contribute to the dioptric apparatus of the eye, as well as its thickness. Spectacle scale transmittance and thickness was found to differ dramatically between snakes and geckos and found in snakes to vary between families. The adaptive significance of the observed variation is discussed. The fourth chapter describes biochemical analyses of the shed spectacle scales of snakes and geckos and compares their composition to other scales in the integument. Spectacle scales were found to differ significantly from other scales in their keratin composition, and gecko spectacle scales in particular were found to lack ß keratin, that hard corneous protein thought to be common to all reptile scales. The concluding chapter will discuss where this research has brought the state of our knowledge on the spectacle and offers thoughts on potentially useful avenues for further research.
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| 132 |
Spatial and temporal dependencies of the motion bridging effect: Investigations of an illusory motionStein, Maximilian 16 December 2019 (has links)
No description available.
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| 133 |
ANALYSES OF THE DEVELOPMENT AND FUNCTION OF STEM CELL DERIVED CELLS IN NEURODEGENERATIVE DISEASES.pdfSailee Sham Lavekar (14152875) 03 February 2023 (has links)
<p>Human pluripotent stem cells (hPSCs) are an attractive tool for the study of different neurodegenerative diseases due to their potential to form any cell type of the body. Due to their versatility and self-renewal capacity, they have different applications such as disease modeling, high throughput drug screening and transplantation. Different animal models have helped answer broader questions related to the physiological functioning of various pathways and the phenotypic effects of a particular neurodegenerative disease. However, due to the lack of success recapitulating some targets identified from animal models into successful clinical trials, there is a need for a direct translational disease model. Since their advent, hPSCs have helped understand various disease effectors and underlying mechanisms using genetic engineering techniques, omics studies and reductionist approaches for the recognition of candidate molecules or pathways required to answer questions related to neurodevelopment, neurodegeneration and neuroregeneration. Due to the simplified approach that iPSC models can provide, some <em>in vitro</em> approaches are being developed using microphysiological systems (MPS) that could answer complex physiological questions. MPS encompass all the different <em>in vitro</em> systems that could help better mimic certain physiological systems that tend to not be mimicked by <em>in vivo</em> models. In this dissertation, efforts have been directed to disease model as well as to understand the intrinsic as well as extrinsic cues using two different MPS. First, we have used hPSCs with Alzheimer’s disease (AD)-related mutations to differentiate into retinal organoids and identify AD related phenotypes for future studies to identify retinal AD biomarkers. Using 5 month old retinal organoids from AD cell lines as well as controls, we could identify retinal AD phenotypes such as an increase in Aβ42:Aβ40 ratio along with increase in pTau:Tau. Nanostring analyses also helped in identification of potential target genes that are modulated in retinal AD that were related to synaptic dysfunction. Thus, using retinal organoids for the identification of retinal AD phenotypes could help delve deeper into the identification of future potential biomarkers in the retina of AD patients, with the potential to serve as a means for early identification and intervention for patients. The next MPS we used to serve to explore non-cell autonomous effects associated with glaucoma to explore the neurovascular unit. Previous studies have demonstrated the degeneration of RGCs in glaucoma due to a point mutation OPTN(E50K) that leads to the degeneration of RGCs both at morphological and functional levels. Thus, using the previous studies as a basis, we wanted to further unravel the impact of this mutation using the different cell types of the neurovascular unit such as endothelial cells, astrocytes and RGCs. Interestingly, we observed the barrier properties being impacted by the mutation present in both RGCs and astrocytes demonstrated through TEER, permeability and transcellular transport changes. We also identified a potential factor TGFβ2 that was observed to be overproduced by the OPTN E50K astrocytes to demonstrate similar effects with the exogenous addition of TGFβ2 on the barrier. Furthermore, the inhibition of TGFβ2 helped rescue some of the barrier dysfunction phenotypes. Thus, TGFβ2 inhibition can be used as a potential candidate that can be used to further study its impact in <em>in vivo</em> models and how that can be used in translational applications. Thus, MPS systems have a lot of applications that can help answer different physiologically relevant questions that are hard to approach using <em>in vivo</em> models and the further development of these systems to accentuate the aspects of neural development and how it goes awry in different neurodegenerative diseases. </p>
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| 134 |
Visuomotor Adaptation During Asymmetric WalkingNapoli, Charles 20 October 2021 (has links) (PDF)
Necessary for effective ambulation, head stability affords optimal conditions for the perception of visual information during dynamic tasks. This maintenance of head-in-space equilibrium is achieved, in part, by the attenuation of the high frequency impact shock resulting from ground contact. While a great deal of experimentation has been done on the matter during steady state locomotion, little is known about how head stability or dynamic visual acuity is maintained during asymmetric walking.
In this study, fifteen participants were instructed to walk on a split-belt treadmill for ten minutes while verbally reporting the orientation of a randomized Landolt-C optotype that was projected at heel strike. Participants were exposed to the baseline, adaptation, and washout conditions, as characterized by belt speed ratios of 1:1, 1:3, and 1:1, respectively. Step length asymmetry, shock attenuation, high (impact) and low (active) frequency head signal power, and dynamic visual acuity scores were averaged across the first and last fifty strides of each condition.
Over the course of the first fifty strides, step length asymmetry was significantly greater during adaptation than during baseline (p d =2.442). Additionally, high frequency head signal power was significantly greater during adaptation than during baseline (p d =1.227), indicating a reduction in head stability. Shock attenuation was significantly lower during adaptation than during baseline (p d =-0.679), and a medium effect size suggests that dynamic visual acuity was lower during adaptation than during baseline as well (p =0.052; d =0.653). When comparing the baseline and adaptation conditions across the last fifty strides, however, many of these decrements were greatly reduced.
The results of this study indicate that the locomotor asymmetry imposed by the split-belt treadmill during the early adaptation condition is responsible for moderate decrements to shock attenuation, head stability, and dynamic visual acuity. Moreover, the relative reduction in magnitude of these decrements across the last fifty strides underscores the adaptive nature of the locomotor and visuomotor systems.
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Accessible Real-time Eye-Gaze Tracking For Neurocognitive Health Assessments, A Multimodal Web-based ApproachTisdale, Daniel C 01 June 2024 (has links) (PDF)
We introduce a novel integration of real-time, predictive eye-gaze tracking models into a multimodal dialogue system tailored for remote health assessments. This system is designed to be highly accessible requiring only a conventional webcam for video input along with minimal cursor interaction and utilizes engaging gaze-based tasks that can be performed directly in a web browser. We have crafted dynamic subsystems that capture high-quality data efficiently and maintain quality through instances of user attrition and incomplete calls. Additionally, these subsystems are designed with the foresight to allow for future re-analysis using improved predictive models, as well as enable the creation and training of new eye-gaze tracking datasets. As we explored gaze patterns for various user-performed tasks, we developed generalizable eye-gaze metrics that capture and reflect the distinct gaze trends among different cohorts. And through testing various feature extraction and classification methods, we have found promising results that have enabled us to effectively classify individuals with Mild Neurocognitive Disorder (MiNCD) / Mild Cognitive Impairment (MCI) in a crowdsourced pilot study (N = 35) with an average accuracy of 0.94 (f1 = 0.83). Although just the beginning, this work represents the first step towards establishing predictive eye-gaze tracking as an accessible and important modality for healthcare applications moving forward, with the potential to significantly impact remote screening and monitoring of neurocognitive health.
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Colour Vision Test for Railway DispatchersRamaswamy, Shankaran 27 April 2009 (has links)
Introduction
Colour codes are used extensively in railways to convey specific information governing movement of trains and equipment on the track. One such task is the railway traffic control display that uses colour coded video display terminals (VDTs) to convey information of the signal status, train movements and track status to the railway dispatcher. Because individuals with colour vision deficiencies (colour-defectives) may have problems with these colour-related tasks, questions were raised about the suitability of colour vision defectives to work as railway dispatchers. In order to answer that, a VDT based Dispatch Colour Vision Test based on the actual railway traffic display was developed previously.
Purpose
The main purpose of this thesis is to establish the pass/fail scores and repeatability of the VDT based Dispatch Colour Vision Test that resulted from the previous work. Secondly, the study will also examine whether clinical colour vision tests can predict the performance on the practical task.
Methods
The Dispatch colour vision test was divided into three parts based on the colour sets that the dispatcher had to recognize. The testing computer system used the the same RGB colour settings, graphics card and monitor as in railway dispatch centres. Subjects viewed the display colours and entered their responses by using a mouse. One hundred colour-normals and fifty two colour-defectives participated in the initial session. The test was repeated approximately after 10 days. Ninety three colour-normals (93%) and 44 (85%) colour-defectives participated in the second session. The total number of errors and time to complete the test was recorded.
Results
Pass/Fail on the VDT Dispatch colour vision test was based on colour-normal errors. Ignoring orange-red errors, two errors were allowed in the first session and one error was allowed in the second session. Based on this criterion, 42% of colour vision defectives could perform as well as colour normal subjects. The kappa coefficient of agreement between the sessions for the colour-defectives was 0.85.
Detailed analysis between the colour differences and the errors showed only a weak correlation between the two. However, the general trend was that colour-defectives made more errors on colours that were near or along the same lines of confusions and the colours were nearly equal in luminance. Nevertheless, the interaction between luminance and location with respect to the lines of confusion was not easy to interpret.
The time to complete the task for the colour-defectives who passed the test took 14% longer than colour-normals and colour-defectives who failed took 30% longer than colour-normals. All groups showed a similar learning effect with an 18% reduction in mean times to complete the task at the second session. There was no significant correlation between the number of errors and time to complete or the clinical tests and completion times for any of the groups.
Clinical colour vision tests have limited value in predicting performance of colour-defectives on the Dispatch test. Logistic analysis results showed that the Farnsworth D-15 along with the Nagel was the best predictor of the VDT Dispatch colour test pass/fail results. However, these results were similar to using the Farnsworth D-15 test alone. Ninety-five percent of the individuals who failed the Farnsworth D-15 also failed the Dispatch test. However, approximately 25% of the individuals who passed the Farnsworth D-15 failed the VDT Dispatch colour test which is an unacceptable false negative rate. These results indicate the Farnsworth D-15 can only be used to predict who is likely to fail the dispatch test.
Conclusions
Forty two percent of colour vision defectives could perform as well as colour-normals in identifying VDT railway display colours and time to complete the task. Clinical colour vision tests were inadequate predictors of performance in practical task, overall. However, the Farnsworth D-15 was a very good predictor of who would fail the VDT Dispatch test. Hence a practical VDT Dispatch test may be needed to test individuals who would want to work as railway dispatchers.
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| 137 |
Colour Vision Test for Railway DispatchersRamaswamy, Shankaran 27 April 2009 (has links)
Introduction
Colour codes are used extensively in railways to convey specific information governing movement of trains and equipment on the track. One such task is the railway traffic control display that uses colour coded video display terminals (VDTs) to convey information of the signal status, train movements and track status to the railway dispatcher. Because individuals with colour vision deficiencies (colour-defectives) may have problems with these colour-related tasks, questions were raised about the suitability of colour vision defectives to work as railway dispatchers. In order to answer that, a VDT based Dispatch Colour Vision Test based on the actual railway traffic display was developed previously.
Purpose
The main purpose of this thesis is to establish the pass/fail scores and repeatability of the VDT based Dispatch Colour Vision Test that resulted from the previous work. Secondly, the study will also examine whether clinical colour vision tests can predict the performance on the practical task.
Methods
The Dispatch colour vision test was divided into three parts based on the colour sets that the dispatcher had to recognize. The testing computer system used the the same RGB colour settings, graphics card and monitor as in railway dispatch centres. Subjects viewed the display colours and entered their responses by using a mouse. One hundred colour-normals and fifty two colour-defectives participated in the initial session. The test was repeated approximately after 10 days. Ninety three colour-normals (93%) and 44 (85%) colour-defectives participated in the second session. The total number of errors and time to complete the test was recorded.
Results
Pass/Fail on the VDT Dispatch colour vision test was based on colour-normal errors. Ignoring orange-red errors, two errors were allowed in the first session and one error was allowed in the second session. Based on this criterion, 42% of colour vision defectives could perform as well as colour normal subjects. The kappa coefficient of agreement between the sessions for the colour-defectives was 0.85.
Detailed analysis between the colour differences and the errors showed only a weak correlation between the two. However, the general trend was that colour-defectives made more errors on colours that were near or along the same lines of confusions and the colours were nearly equal in luminance. Nevertheless, the interaction between luminance and location with respect to the lines of confusion was not easy to interpret.
The time to complete the task for the colour-defectives who passed the test took 14% longer than colour-normals and colour-defectives who failed took 30% longer than colour-normals. All groups showed a similar learning effect with an 18% reduction in mean times to complete the task at the second session. There was no significant correlation between the number of errors and time to complete or the clinical tests and completion times for any of the groups.
Clinical colour vision tests have limited value in predicting performance of colour-defectives on the Dispatch test. Logistic analysis results showed that the Farnsworth D-15 along with the Nagel was the best predictor of the VDT Dispatch colour test pass/fail results. However, these results were similar to using the Farnsworth D-15 test alone. Ninety-five percent of the individuals who failed the Farnsworth D-15 also failed the Dispatch test. However, approximately 25% of the individuals who passed the Farnsworth D-15 failed the VDT Dispatch colour test which is an unacceptable false negative rate. These results indicate the Farnsworth D-15 can only be used to predict who is likely to fail the dispatch test.
Conclusions
Forty two percent of colour vision defectives could perform as well as colour-normals in identifying VDT railway display colours and time to complete the task. Clinical colour vision tests were inadequate predictors of performance in practical task, overall. However, the Farnsworth D-15 was a very good predictor of who would fail the VDT Dispatch test. Hence a practical VDT Dispatch test may be needed to test individuals who would want to work as railway dispatchers.
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| 138 |
Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic ProductsMcCanna, David January 2009 (has links)
The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty associated with dose selection for clinical trials. The first section of this thesis describes how in vitro methods can be used to improve the prediction of the toxicity of chemicals and ophthalmic products. The proper utilization of in vitro methods can accurately predict toxic threshold levels and reduce animal use in product development. Sections two, three and four describe the development of new sensitive in vitro methods for predicting ocular toxicity. Maintaining the barrier function of the cornea is critical for the prevention of the penetration of infections microorganisms and irritating chemicals into the eye. Chapter 2 describes the development of a method for assessing the effects of chemicals on tight junctions using a human corneal epithelial and canine kidney epithelial cell line. In Chapter 3 a method that uses a primary organ culture for assessing single instillation and multiple instillation toxic effects is described. The ScanTox system was shown to be an ideal system to monitor the toxic effects over time as multiple readings can be taken of treated bovine lenses using the nondestructive method of assessing for the lens optical quality. Confirmations of toxic effects were made with the utilization of the viability dye alamarBlue. Chapter 4 describes the development of sensitive in vitro assays for detecting ocular toxicity by measuring the effects of chemicals on the mitochondrial integrity of bovine cornea, bovine lens epithelium and corneal epithelial cells, using fluorescent dyes.
The goal of this research was to develop an in vitro test battery that can be used to accurately predict the ocular toxicity of new chemicals and ophthalmic formulations. By comparing the toxicity seen in vivo animals and humans with the toxicity response in these new in vitro methods, it was demonstrated that these in vitro methods can be utilized in a tiered testing strategy in the development of new chemicals and ophthalmic formulations.
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| 139 |
Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic ProductsMcCanna, David January 2009 (has links)
The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty associated with dose selection for clinical trials. The first section of this thesis describes how in vitro methods can be used to improve the prediction of the toxicity of chemicals and ophthalmic products. The proper utilization of in vitro methods can accurately predict toxic threshold levels and reduce animal use in product development. Sections two, three and four describe the development of new sensitive in vitro methods for predicting ocular toxicity. Maintaining the barrier function of the cornea is critical for the prevention of the penetration of infections microorganisms and irritating chemicals into the eye. Chapter 2 describes the development of a method for assessing the effects of chemicals on tight junctions using a human corneal epithelial and canine kidney epithelial cell line. In Chapter 3 a method that uses a primary organ culture for assessing single instillation and multiple instillation toxic effects is described. The ScanTox system was shown to be an ideal system to monitor the toxic effects over time as multiple readings can be taken of treated bovine lenses using the nondestructive method of assessing for the lens optical quality. Confirmations of toxic effects were made with the utilization of the viability dye alamarBlue. Chapter 4 describes the development of sensitive in vitro assays for detecting ocular toxicity by measuring the effects of chemicals on the mitochondrial integrity of bovine cornea, bovine lens epithelium and corneal epithelial cells, using fluorescent dyes.
The goal of this research was to develop an in vitro test battery that can be used to accurately predict the ocular toxicity of new chemicals and ophthalmic formulations. By comparing the toxicity seen in vivo animals and humans with the toxicity response in these new in vitro methods, it was demonstrated that these in vitro methods can be utilized in a tiered testing strategy in the development of new chemicals and ophthalmic formulations.
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