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<p>  </p> <p>Mutations drive evolution, generating variation that selection can act upon. Germline mutations are heritable genetic alterations that occur in the gametes prior to fertilization and until embryogenesis. The study of germline mutations is vital to understanding the genetic basis of heritable diseases and evolution. Somatic mutations are genetic alterations across the body that arise post-fertilization in non-gametic cells. Although somatic mutations in most animals cannot be inherited, they can still significantly affect an organism's reproductive success. In humans, for example, cancers can be the result of somatic mutations. Somatic mutations originate from both exogenous mutagens (e.g. UV radiation) and endogenous processes (e.g. DNA replication, aging). Beyond their origins, we know little about the distribution and frequency of somatic mutations across Animalia. Honey bees provide a unique model for the study of somatic mutations as they are haplodiploid: males come from unfertilized eggs and are haploid, while females come from fertilized eggs and are diploid. It is therefore possible to sequence and robustly identify somatic mutations in haploid males. I have developed a unique exploratory study to elucidate the distribution of somatic mutation accumulation in honey bee drones. With this, I aim to investigate processes generating somatic mutations. Our findings demonstrate that variance in somatic mutational load is better captured across individuals rather than within individuals (across tissues). I provide a comprehensive tissue atlas of somatic mutagenesis in haploids. Our findings drive us to enhance our view of mutagenesis from the tissue level down to the cellular level. </p>

The aim of this thesis is to break new ground by investigating the interactional organisation of real events that comprise live business-to-business cold calls. Despite being a ubiquitous part of everyday life, we know very little about how cold calls are initiated, progressed, and completed. Cold calls are unsolicited telephone encounters, initiated by salespeople aiming to get prospective clients ( prospects ) interested in their services, with the distal goal of turning them into clients and the proximal goal of getting them to agree to an initial meeting. Cold calls are often treated as a nuisance by call-takers, and salespeople must deal with reluctant gatekeepers, recurrent sales resistance, and the occasional hang-up. The training they receive often draws on outdated theories of communication and is rarely supported by empirical evidence. Thus, this study not only addresses an important domain for interactional research, but also fulfils a practical necessity for empirical research that will inform sales training and improve callers and call-takers experiences. The data comprise 150 recorded calls supplied by three British companies that sell, service, and lease office equipment. The data were collected, transcribed, and analysed within an ethnomethodological framework using conversation analysis and discursive psychology. The first analytic chapter outlines the overall structural organisation of cold calling. It documents the constituent activities within the opening, the business of the call, and the closing. It identifies and describes two types of cold calls. Freezing calls are initiated by salespeople who are contacting a prospect for the very first time. Lukewarm calls feature salespeople who claim to have been in contact with the prospect s organisation in the past. The second chapter excavates the initial turns of lukewarm calls in which salespeople ask to speak to another person within the company, with whom they indicate to be acquainted. The analysis revealed that this third-party acquaintanceship was crucial for establishing the legitimacy of the switchboard request and for improving the chances of getting it granted. The third chapter focused on appointment-making sequences in both freezing and lukewarm calls, showing that they comprise two components: a preamble and a meeting request sequence. I also highlight how salespeople exploit sequential and turn-taking mechanisms to secure meetings with prospects without giving the latter the opportunity to refuse. The final chapter examines two practices for enacting resistance in cold calls blocks and stalls and documents the range of methods salespeople employ for dealing with each type of resistance. Sales blocks expose the salesperson s commercial agenda, attempt to stop the prospecting activity, and move towards call pre-closure. In response, salespeople can challenge, counter, or circumvent blocks as well as redo their initiating actions. Stalls slow down the progress of the sales process by delaying the next phase of the sale or by proposing less commitment-implicative alternatives. Salespeople deal with stalls by either justifying their initial proposal or by spontaneously introducing new action plans, both being more conducive to the progress of the sale. The thesis contributes to a growing body of interactional research on commercial encounters by shedding empirical light on a previously unexamined setting, business-to-business cold calls. It also moves forward discursive psychology s project of respecifying psychological phenomena by documenting the communicative practices associated with persuasion and resistance. Finally, it expands the extant conversation analytic toolkit by examining new practices (such as appointment-making) and by providing new insights into key conversation analytic topics (such as requests, pre-sequences, and accounts for calling). Overall, the findings presented in this thesis challenge existing conceptions of prospecting through cold calling that are prevalent in the sales literature. The thesis puts forward a strong argument for opening the black box of cold calls to better understand these interactions and to identify good practices as the basis for communication training. Research presented in this thesis has already been used in the development of CARM (Conversation Analytic Role-play Method) training for salespeople, who reported having doubled their appointment rates. Based on the findings in this thesis, I plan to develop further training not only for salespeople but also for prospective customers, thus improving the overall outcome of cold call encounters.

Text in Afrikaans / In this dissertation, different aspects of aChurch leadership" are examined from a Pentecostal perspective. The framework is composed of literature compiled over the past twenty years. Especially the relationship between Church Leadership and the working of the Holy Spirit comes under the searchlight. The Theological theory underlying the Church Leadership exponents as well that of the Pentecostal movement are developed and critically evaluated. The Pentecostal ideal is that church leadership will function as leadership within a church context under the guidance and "working of the Holy Spirit The Pentecostal movement emphasises that the working of the Holy Spirit is the criticalfactor determining the effectiveness of the church. In the literature en Church leadership, the main focus is placed on the key role of church leadership with specific reference to the functional aspects and skills that church leaders need to be effective. The implication of both the aforementioned points are discussed and compared as well as the possible implications for both are indicated. Systematic theological issues concerning the role of persons in specific offices in different church traditions are avoided. It is stated that in the literature on Church Leadership more emphasis should be placed in the Working of the Holy Spirit as the critical factor in church leadership. It is clear that there is not enough emphasis on the working of the Holy Spirit if taken into account the importance thereof for the church as a whole. An approach to church leadership as "spiritual leadership" could assist in future research on this matter. In chapter one the framework of this study is explained. Chapter two reflects on the development of the Pentecostal perspective and chapter three on the Church leadership perspective. The different topics, similarities, critical reflections, evaluations and recommendations are presented in the last chapter. / In hierdie proefskrif word daar vanuit 'n Pinksterperspektief 'n ondersoek gedoen na kerkleierskap. Die raamwerk is literatuur wat in die afgelope twintig jaar oor hierdie onderwerp na vore gekom het. Veral die verband wat aangebied word tussen kerkleierskap en die werking van die Heilige Gees, kom onder die soeklig. Die teologiese teoriee onderliggend aan die standpunte van die kerkleierskap-eksponente sowel as die van die Pinksterbeweging word ontwikkel en krities beoordeel. Die Pinksterideaal is dat kerkleierskap gekenmerk sal word as leierskap wat funksioneer onder die Ieiding en werking van die Heilige Gees. Die Pinksterbeweging beklemtoon dat die werking van die Heilige Gees die deurslaggewende faktor is vir die effektiewe werking en ontwikkeling van die kerk. In die literatuur oor kerkleierskap word daarenteen hoofsaaklik klem geplaas op die sleutelrol van kerkleierskap met spesifieke verwysing na die funksionele aspekte en vaardighede wat kerkleiers benodig met die oog op die uitbouing van die kerk. Die implikasies van beide beklemtoninge word ontwikkel en met mekaar vergelyk en moontlike implikasies vir beide word aangetoon. Sistematies-teologiese vraagstukke rakende die rot van persona in bepaalde ampte in verskillende kerklike tradisies word vermy. Dit word beredeneer dat daar in die literatuur oar die onderhawige onderwerp meer klem geplaas behoort te word op die werking van die Heilige Gees as die deurslaggewende faktor vir suksesvolle kerkleierskap. Dit is duidelik dat daar nie genoegsaam gefokus word op die werking van die Heilige Gees nie ten spyte van die belangrikheid daarvan vir die kerklike praktyk in geheel. Die moontlikheid om kerkleierskap eerder te omskryf en te benader as "geestelike leierskap” bied moontlikhede ten opsigte van toekomstige navorsing op die gebied. In hoofstuk een worrd die raamwerk waarbinne die studie ontwikkel uiteengesit. Hoofstuk twee bied die basis waarbinne die Pinksterstandpunt ontwikkel het terwyl hoofstuk drie die onderwerp vanuit 'n kerkleierskapsperspektief benader. Klemverskille, ooreenkomste, kritiese besprekings, evaluering en aanbevelings word in die laaste hoofstuk aangebied. / Practical Theology / D.Th. (Practical Theology)

The justification of property ownership based on individual subjective rights is tightly bound to humanist moral perspectives. God is left out as irrelevant to the just grounds of ownership, which is established primarily on the basis of human self-referential, moral capacity. This thesis aims at developing an alternative justification, both for property as an institution and as a private holding, with a view to bringing God back into the centre stage and thereby placing property ownership on the objective concept of right. A tradition hitherto generally left unnoticed, yet uncovered here as the source of inspiration, vests the whole project with a moral-teleological tone. The tradition, enunciated by St. Augustine and developed by St. Bonaventure and John Wyclif, invites us to see property from the perspective of a moral end: it ought to be used for the love of God and neighbours, and as such it can be owned only by the just. In spite of important insights into the moral nature of property, the Augustinian thesis not only fails to spell out what ‘use for love’ means but also suffers from elitism. Nor does it offer an adequate justification of private property. Such weaknesses call for revision. When we reinterpret the Augustinian thesis through the concept of the divine imperative of service coupled with a proper understanding of human work, property acquires a distinctive justification. Property, as an institution, is justified as a requisite for carrying out God’s redemptive work towards the world. From this general justification ensues the particular justification. We hold property as specifically ‘mine,’ since each person’s ordained mission to participate in God’s work requires a uniquely personal material means, although the recognition and fulfilment of individual mission still demands communal efforts. The duty to carry out the God-commanded mission at first allows us to possess private property only in a non-proprietorial and non-exclusive manner. Yet in the prevailing condition of economic scarcity and human greed, civil jurisdiction must provide a structure of rights to enforce property institution. As God’s invitation for the transformation of the world is a universal command, everybody should have a minimum of property, and yet in differentiation of the scope and kinds commensurate with the particularities of individual mission.

This thesis represents a comprehensive examination of the phonotactic behaviour (i.e. attraction to sound) of the female Gryllus bimaculatus under laboratory conditions. Chapter 2 is the first study to analyze the effect of substrate texture on walking performance in crickets. Substrate texture is found to play an essential role in the phonotactic responses of G. bimaculatus. Smooth substrate texture has a detrimental effect due to slipping, whereas a rough texture results in optimal walking performance due to the friction with the walking legs. Chapter 3 represents the first detailed lifetime study analysing phonotaxis in crickets. My results demonstrate that the optimal age to test phonotaxis in G. bimaculatus females is from day 7 to 24 after the final moult. I also found that selectiveness was persistent with age. These findings contradict the female choosiness hypothesis. This study is also the first to describe the effect of senescence on phonotaxis in insects, as responsiveness decreases with age. Chapter 4 compares the phonotactic behaviour of female crickets from different laboratory-bred colonies. From six tested cricket lab colonies, I found three groups statistically different from each other. Females raised under laboratory conditions at the University of Cambridge and Anglia Ruskin University were most reponsive at a frequency of 4.5 kHz, whereas females bred in Tokushima University in Japan were tuned towards a higher frequency of 5 kHz. These results suggest a degree of artificial allopatric speciation. Comparisons with crickets bred under low-quality conditions in a local pet shop demonstrate a loss of responsiveness, indicating that breeding conditions have a direct effect on phonotactic responsivity. Chapter 5 is the first study to report the presence of phonotaxis in males of G. bimaculatus. Previously it was unknown if G. bimaculatus males were able to perform phonotaxis, given that they were only recognised as endurance signal producers. In the present study, only 20% of the studied males (N=70) performed a weak phonotactic response. This finding has potential ecological implications in terms of male cricket territory establishment, and male-male interactions in the wild, which are discussed. Chapter 6 explores the song pattern recognition of the female G. bimaculatus by changing the duration of either the first, second or third pulse of the chirps. A long first pulse decreased the phonotactic response whereas phonotaxis remained strong when the third pulse was long. Chirps with three pulses of increasing duration of 5, 20 and 50 ms elicited phonotaxis, but the chirps were not attractive when played in reverse order. The data are in agreement with a mechanism in which processing of a sound pulse has an effect on the processing of the subsequent pulse, as outlined in the flow of activity in a delay-line and coincidence-detector circuit.

This MA thesis analyzes how the protagonists and the narrative structure relates to “the Child” as a linguistic/cultural construction in Mare Kandre’s early authorship (1984-1991). A thematic of children portraits defines this period of Kandre’s writing, which consits of the following works: In a Different Country (1984), The Annunciation (1986), Bübin’s Kid (1987), The Burning Tree (1988) and Aliide, Aliide (1991). Linguistic components, such as the word “child”, the name “Kid” and a reconstruction of the concept of childhood, holds an actuate position in these narratives when they first appear in direct relation to the protagonists. This suggests how generally accepted categories and concepts consciously are at work in Kandre’s writings. Consequently, a category such as “child” can not unproblematically serve as a definition of any of the protagonists, irrespective of their presumptive age. Combining philosopher Judith Butler’s theory of name-calling with theories in the field of Childhood Studies as presented by Susan Honeyman, “the Child” in Kandre’s works is explored through the hypothesis of its function as a name. Close readings reveal how the protagonists, in the encounter with an explicitly cultural or narrative context, are constituted as subjects through the linguistic category “child”. The protagonists can be considered both as subordinated by the conventionally manifested notion of life development as a linear, measurable progress and as introduced to the means which make possible a critical response to that same notion. Since the main protagonists strongly refuse to be defined by the conventions supporting “the Child” as a category, a dialogue is created which gives the narrative its force. That dialogue corresponds to different aspects of the concept of childhood, which place “the Child” as a construction at the heart of Kandre’s narrative composition.

New approaches are needed to manage and analyze the enormous quantity of biological data generated by modern technologies. Existing solutions are often fragmented and uncoordinated and, thus, they require considerable bioinformatics skills from users. Three applications have been developed illustrating different strategies to help users without extensive IT knowledge to take maximum profit from their data. SNPator is an easy-to-use suite that integrates all the usual tools for genetic association studies: from initial quality control procedures to final statistical analysis. CHAVA is an interactive visual application for CNV calling from aCGH data. It presents data in a visual way that helps assessing the quality of the calling and assists in the process of optimization. Haplotype Association Pattern Analysis visually presents data from exhaustive genomic haplotype associations, so that users can recognize patterns of possible associations that cannot be detected by single-SNP tests. / Calen noves aproximacions per la gestió i anàlisi de les enormes quantitats de dades biològiques generades per les tecnologies modernes. Les solucions existents, sovint fragmentaries i descoordinades, requereixen elevats nivells de formació bioinformàtica. Hem desenvolupat tres aplicacions que il•lustren diferents estratègies per ajudar als usuaris no experts en informàtica a aprofitar al màxim les seves dades. SNPator és un paquet de fàcil us que integra les eines usades habitualment en estudis de associació genètica: des del control de qualitat fins les anàlisi estadístiques. CHAVA és una aplicació visual interactiva per a la determinació de CNVs a partir de dades aCGH. Presenta les dades visualment per ajudar a valorar la qualitat de les CNV predites i ajudar a optimitzar-la. Haplotype Pattern Analysis presenta dades d’anàlisi d’associació haplotípica de forma visual per tal que els usuaris puguin reconèixer patrons de associacions que no es possible detectar amb tests de SNPs individuals.

Text in Afrikaans / In this dissertation, different aspects of aChurch leadership" are examined from a Pentecostal perspective. The framework is composed of literature compiled over the past twenty years. Especially the relationship between Church Leadership and the working of the Holy Spirit comes under the searchlight. The Theological theory underlying the Church Leadership exponents as well that of the Pentecostal movement are developed and critically evaluated. The Pentecostal ideal is that church leadership will function as leadership within a church context under the guidance and "working of the Holy Spirit The Pentecostal movement emphasises that the working of the Holy Spirit is the criticalfactor determining the effectiveness of the church. In the literature en Church leadership, the main focus is placed on the key role of church leadership with specific reference to the functional aspects and skills that church leaders need to be effective. The implication of both the aforementioned points are discussed and compared as well as the possible implications for both are indicated. Systematic theological issues concerning the role of persons in specific offices in different church traditions are avoided. It is stated that in the literature on Church Leadership more emphasis should be placed in the Working of the Holy Spirit as the critical factor in church leadership. It is clear that there is not enough emphasis on the working of the Holy Spirit if taken into account the importance thereof for the church as a whole. An approach to church leadership as "spiritual leadership" could assist in future research on this matter. In chapter one the framework of this study is explained. Chapter two reflects on the development of the Pentecostal perspective and chapter three on the Church leadership perspective. The different topics, similarities, critical reflections, evaluations and recommendations are presented in the last chapter. / In hierdie proefskrif word daar vanuit 'n Pinksterperspektief 'n ondersoek gedoen na kerkleierskap. Die raamwerk is literatuur wat in die afgelope twintig jaar oor hierdie onderwerp na vore gekom het. Veral die verband wat aangebied word tussen kerkleierskap en die werking van die Heilige Gees, kom onder die soeklig. Die teologiese teoriee onderliggend aan die standpunte van die kerkleierskap-eksponente sowel as die van die Pinksterbeweging word ontwikkel en krities beoordeel. Die Pinksterideaal is dat kerkleierskap gekenmerk sal word as leierskap wat funksioneer onder die Ieiding en werking van die Heilige Gees. Die Pinksterbeweging beklemtoon dat die werking van die Heilige Gees die deurslaggewende faktor is vir die effektiewe werking en ontwikkeling van die kerk. In die literatuur oor kerkleierskap word daarenteen hoofsaaklik klem geplaas op die sleutelrol van kerkleierskap met spesifieke verwysing na die funksionele aspekte en vaardighede wat kerkleiers benodig met die oog op die uitbouing van die kerk. Die implikasies van beide beklemtoninge word ontwikkel en met mekaar vergelyk en moontlike implikasies vir beide word aangetoon. Sistematies-teologiese vraagstukke rakende die rot van persona in bepaalde ampte in verskillende kerklike tradisies word vermy. Dit word beredeneer dat daar in die literatuur oar die onderhawige onderwerp meer klem geplaas behoort te word op die werking van die Heilige Gees as die deurslaggewende faktor vir suksesvolle kerkleierskap. Dit is duidelik dat daar nie genoegsaam gefokus word op die werking van die Heilige Gees nie ten spyte van die belangrikheid daarvan vir die kerklike praktyk in geheel. Die moontlikheid om kerkleierskap eerder te omskryf en te benader as "geestelike leierskap” bied moontlikhede ten opsigte van toekomstige navorsing op die gebied. In hoofstuk een worrd die raamwerk waarbinne die studie ontwikkel uiteengesit. Hoofstuk twee bied die basis waarbinne die Pinksterstandpunt ontwikkel het terwyl hoofstuk drie die onderwerp vanuit 'n kerkleierskapsperspektief benader. Klemverskille, ooreenkomste, kritiese besprekings, evaluering en aanbevelings word in die laaste hoofstuk aangebied. / Philosophy, Practical and Systematic Theology / D.Th. (Practical Theology)

Text in German / Die vorliegende Arbeit ist der Kybernetik zuzuordnen, einer Unterdisziplin der Praktischen Theologie. In einer empirischen Untersuchung wird Führungsangst im Pastorendienst am Beispiel von Pastoren im Bund Freier evangelischer Gemeinden erforscht. Ausgangspunkt ist die Frage, welche Ängste Pastoren beim Führen haben. Dabei wird nach Ursachen, Erklärungen und Folgen dieser Ängste gefragt. Ziel ist, den Einfluss von Angst im Führungsverhalten von Pastoren besser zu verstehen, um damit bewusster umgehen zu können. Das Literatur-Kapitel erklärt Begriffe und stellt relevante Literatur dar. Dabei werden Angstklassifikationen, Angstabwehrmechanismen sowie systembedingte und individuelle Angstauslöser näher gefasst. In der empirischen Forschung wurden erfahrene Pastoren größerer Freien evangelischer Gemeinden interviewt. Die Datenanalyse erfolgte mittels der Methode Grounded Theory. Macht und Angst wurden als Tabu-Themen deutlich, die wenig reflektiert werden. Sie führen zu Konflikten, Machtkämpfen und zu überforderten, kranken oder gemobbten Pastoren. Die Arbeitshypothese wurde bestätigt: Führungsangst ist Pastoren nicht fremd. Allerdings ist sie eine verdrängte Form ihrer Arbeitsrealität und sozialen Wirklichkeit. / From within the field of cybernetics, a sub-discipline of Practical Theology, the fear for roles of leadership in pastoral ministry is examined empirically, through interviewing pastors of the Covenant of Free Evangelical Churches in Germany. The specific fears pastors experience when leading congregations, as well as the causes and effects of these fears, are examined from relevant sources of literature. The classification of fear and defense mechanisms, as well as system-specific and individual-specific triggers, are also discussed. For the empirical part, experienced pastors of larger Free Evangelical Churches were interviewed and data was analyzed according to the Grounded Theory method. Power and fear are identified as factors which can lead to conflict and power struggles and to overworked or ill pastors. Seen as taboo topics, pastors tend to ignore these issues. The study shows that the fear for leadership, although repressed, is a reality in the lives of pastors. / Philosophy, Practical and Systematic Theology / M. Th. (Christian Leadership)