This work aimed to evaluate the digestibility of ingredients and estimate the nutritional requirement of methionine for juvenile catfish (Rhamdia quelen). For this a digestibility trial was carried out by determining the apparent digestibility coefficients (ADC) of dry matter (DM), crude protein (CP) and gross energy (GE) of ingredients: catfishes flour (FJ), commercial fishmeal (FPC) of meat and bone meal (MBM), soybean protein concentrate (SPC), maltodextrin (M), corn starch (AM), gelatin (G) and casein (C) for juvenile catfish (weight 35.45 ± 12.1 g), through a reference diet (RD) and test diets (diet with 70% of DR over 30% of the tested ingredient) containing 0.3% chromic oxide as an inert marker. The digestibility coefficients were adjusted to compensate for the partial loss of nutrients through leaching by water. Ingredients test FPC, FJ, M, C and G showed higher DM, 77.03 to 95.66%, for PB the CPS showed lower digestibility (59.70%) compared to the other ingredients FPC, FCO, FJ, G and C were 87.19 to 99.59%, as well as for EB the CPS showed lower digestibility 71.59% did not differ AM with 73,59%, being the ingredients FCO, FJ, FPC, G and C with higher digestibility, with 82.70 to 85.79%. Subsequently a dose response test was conducted to estimate the methionine requirement of juvenile catfish (initial weight of 3.26 ± 0.3 g and medium length 7.17 ± 0.27 cm), using 35 animals per experimental unit, and four replications. Six isoproteic and isocaloric diets were formulated with six increasing levels of methionine (0.9, 1.6, 2.3, 3.0, 3.7 and 4.4% crude protein) holding constant the level of cystine (0 , 19% in crude protein). Each diet for fish were distributed randomly, provided twice daily (9:30 and 17:00) until apparent satiation, during 15 weeks. The values of relative weight gain (RWG) and specific growth rate (SGR) increased up to 3.0% of methionine (P <0.05) and being kept virtually the same later. The rate of protein retention increased up to 2.3% methionine level, but decreasing in the 4.4% level (P <0.05). The quadratic regression analysis on the RWG and SGR indicated that optimal levels of methionine in the diet recommended for the growth of juvenile catfish, are estimated at 3.59% and 3.46% in crude protein, respectively, and maintaining a constant level of cystine (0.19% in crude protein), thus corresponding to 1.33 and 1.28% in the diet, or total sulfur amino acid to 3.65 and 3.78% in the crude protein, respectively. / Este trabalho teve por objetivo avaliar a digestibilidade de ingredientes e estimar a exigência nutricional em metionina para juvenis de jundiá (Rhamdia quelen). Para isso realizou-se um ensaio de digestibilidade determinando os coeficientes de digestibilidade aparente (CDA) da matéria seca (MS), proteína bruta (PB) e energia bruta (EB), dos ingredientes: farinha de jundiá (FJ), farinha de peixe comercial (FPC), farinha de carne e ossos (FCO), concentrado protéico de soja (CPS), maltodextrina (M), amido de milho (AM), gelatina (G) e caseína (C), para juvenis de jundiá (peso 35,45±12,1 g), através de uma dieta referência (DR) e dietas-teste (dieta com 70% de DR mais 30% do ingrediente testado) contendo 0,3% de óxido de cromo como marcador inerte. Os coeficientes de digestibilidade foram ajustados para compensar as perdas parciais dos nutrientes por lixiviação pela água. Os ingredientes teste FPC, FJ, M, C e G apresentaram maior digestibilidade da MS, de 77,03 a 95,66%, já para PB o CPS apresentou menor digestibilidade (59,70%) comparado aos demais ingredientes FPC, FCO, FJ, G e C que foram de 87,19 a 99,59%, assim como para EB o CPS apresentou menor digestibilidade 71,59% não diferindo do AM com 73,59%, sendo os ingredientes FCO, FJ, FPC, G e C com maior digestibilidade, apresentando 82,70 a 85,79%. Posteriormente realizou-se ensaio de dose resposta para estimar a exigência de metionina de juvenis de jundiás (peso médio inicial de 3,26±0,3 g e comprimento médio 7,17±0,27 cm), utilizando 35 animais por unidade experimental, e quatro repetições. Seis rações isoproteica e isoenergética foram formuladas com seis níveis crescentes de metionina (0,9; 1,6; 2,3; 3,0; 3,7 e 4,4% na proteína bruta) mantendo constante o nível de cistina (0,19% na proteína bruta). Cada dieta foi distribuída aleatoriamente aos peixes, fornecida duas vezes ao dia (09:30 e 17:00 h), até a saciedade aparente, por 15 semanas. Os valores de ganho em peso relativo (GPR) e taxa de crescimento específico (TCE) aumentaram até o nível de metionina 3,0% (P<0,05) e mantendo-se praticamente o mesmo posteriormente. A taxa de retenção proteica aumentou até o nível de metionina 2,3%, mas diminuindo no nível 4,4% (P<0,05). A análise de regressão quadrática sobre o GPR e TCE indicou que os níveis ideais de metionina na dieta, recomendadas para o crescimento de juvenis de jundiá, são estimados em 3,59% e 3,46% na proteína bruta, respectivamente, mantendo o nível de cistina constante (0,19% na proteína bruta), correspondendo assim a 1,33 e 1,28% na dieta, ou um total de aminoácido sulfurado de 3,78 e 3,65% na proteína bruta, respectivamente.

Made available in DSpace on 2015-04-17T14:49:31Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 2700289 bytes, checksum: 3ce6dace6dd9e56c5d2e89144a1d467b (MD5) Previous issue date: 2013-08-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Aquaculture has become one of the activities that most expands globally, with shrimp L. van-namei contributed to the breakthrough and growth of shrimp farming, especially in the North-east in Brazil. Feeding shrimp is one of the main aspects to be considered in its cultivation, representing costs up to 60% in production, it is important to use new alternatives that mini-mize the costs of fishmeal and pressure on fish stocks already limited. Was evaluated in three experiments, the phases of post-larvae and fattening marine shrimp in intensive system, the use of Spirulina platensis as a protein source in replacement of fishmeal and supplementation in artificial diets. In the first experiment, post-larvae of Litopenaeus vannamei (0.74 g) were fed exclusively on S. platensis in liquid form and concentrate, in the second experiment was also used post-larvae with the same initial weight, however the S. platensis in lyophilized form was added to experimental diets isoproteic (40%) and isoenergetic (3,200 kcal), with different levels of protein replacement of fishmeal by S. platensis (0, 25, 50 and 100%), both for a period of 30 days, and assessing the productive performance of prawns. In the third ex-periment, we used juvenile shrimp (1.42 g) for 45 days diets containing different proportions of S. platensis lyophilized (0%, 10%, 20%, 30% and 40%), and also isoenergetic and isopro-teic (35% crude protein and 3.400Kcal), also evaluating the performance and proximate com-position of the muscle of the shrimp. In all experiments we used a control diet for comparison, being provided ad libitum. Were also evaluated in all experiments the physical and chemical variables of water considered of major importance in the cultivation (pH, temperature, salinity and dissolved oxygen). The experiments were conducted at the Center for Research and Pro-cessing food -UFPB, with a completely randomized design with different replicates in each, using a system of rectangular tanks with a capacity of 30 liters and aeration still, filled with sea water mixed with drinking water (no chlorine) to obtain the desired salinity. The physical and chemical parameters of the water remained within the limits acceptable to the develop-ment of Litopenaeus vannamei at all stages of creation in the experiments, showing no signif-icant differences between the different treatments. The results, we observed a higher efficien-cy in productive performance of shrimp fed with S. platensis in all experiments, showing bet-ter growth rates with elevated survival in the reduction of fish meal by up to 25%, demonstrat-ing a better amino acid profile with supplementation of 40% in diets for shrimp, providing better results in protein and lipid composition in muscle tissue of shrimp without compromis-ing the level of cholesterol, demonstrating the influence of diet on the fatty acid profile in nutritional quality for human consumption. / A aquicultura tem se tornado uma das atividades que mais se expande mundialmente, tendo o camarão L. vannamei contribuído para o grande avanço e crescimento da carcinicultura, prin-cipalmente na região Nordeste no Brasil. A alimentação do camarão constitui um dos princi-pais aspectos a ser considerado na sua criação, representando custos de até 60% na produção, sendo importante a utilização de novas alternativas que minimizem a os custos com a farinha de peixe e a pressão sobre os estoques pesqueiros já limitados. Foi avaliado em três experi-mentos, nas fases de pós-larvas e de engorda do camarão marinho em sistema intensivo, o uso da microalga Spirulina platensis como fonte proteica, na substituição da farinha de peixe e na suplementação em dietas artificiais. No primeiro experimento, pós-larvas do Litopenaeus vannamei (0,74g) foram alimentadas exclusivamente com S. platensis na forma liquida e ra-ção comercial; no segundo experimento utilizou-se também pós-larvas com o mesmo peso inicial, entretanto a S. platensis na forma liofilizada foi adicionada em dietas experimentais isoproteicas (40%) e isoenergeticas (3.200 Kcal), com diferentes níveis de substituição da proteína da farinha de peixe pela S. platensis (0, 25, 50 e 100%), ambos por um período de 30 dias, e avaliando o desempenho produtivo dos camarões. No terceiro experimento, foi utiliza-do camarões juvenis (1,42g) durante 45 dias com dietas formuladas com diferentes propor-ções da S. platensis liofilizada (0%, 10%, 20%, 30% e 40%), sendo também isoproteicas e isoenergeticas (35% Proteína bruta e 3.400Kcal), avaliando também o desempenho zootécni-co e a composição centesimal do músculo do camarão. Em todos os experimentos utilizou-se uma ração controle para a comparação, sendo fornecidas ad libitum. As variáveis físicas e químicas da água foram avaliadas sendo consideradas de maior importância no cultivo (pH, temperatura, salinidade e oxigênio dissolvido). Os experimentos foram realizados no Núcleo de Pesquisa e Processamento de Alimentos-UFPB, com um delineamento casualizado apre-sentando diferentes repetições em cada um, utilizando um sistema de aquários retangulares com capacidade para 30 litros e aeração continua, abastecidos com água do mar misturada a água potável (sem cloro) até a obtenção da salinidade desejada. Os parâmetros físicos e quí-micos da água se mantiveram entre os limites aceitáveis para o desenvolvimento do Litope-naeus vannamei em todas as fases de criação nos experimentos, não apresentando diferenças significativas entre os diferentes tratamentos. Nos resultados obtidos, foi observada uma mai-or eficiência no desempenho produtivo dos camarões alimentados com a S. platensis em todos os experimentos, mostrando melhores taxas de crescimento com elevada sobrevivência na redução da farinha de peixe em até 25%, demonstrando um melhor perfil de aminoácidos com a suplementação de 40% nas rações para o camarão, proporcionando melhores resultados na composição proteína e lipídica no tecido muscular do camarão sem comprometer o teor de colesterol, demonstrando a influência da dieta no perfil de ácidos graxos na qualidade nutrici-onal para o consumo humano.

<p>Modern food production is a complex, globalized system in which what we eat and how it is produced are increasingly disconnected. This thesis examines some of the ways in which global trade has changed the mix of inputs to food and feed, and how this affects food security and our perceptions of sustainability. One useful indicator of the ecological impact of trade in food and feed products is the Appropriated Ecosystem Areas (ArEAs), which estimates the terrestrial and aquatic areas needed to produce all the inputs to particular products.</p><p>The method is introduced in Paper I and used to calculate and track changes in imported subsidies to Swedish agriculture over the period 1962-1994. In 1994, Swedish consumers needed agricultural areas outside their national borders to satisfy more than a third of their food consumption needs. The method is then applied to Swedish meat production in Paper II to show that the term “Made in Sweden” is often a misnomer. In 1999, almost 80% of manufactured feed for Swedish pigs, cattle and chickens was dependent on imported inputs, mainly from Europe, Southeast Asia and South America. Paper III examines ecosystem subsidies to intensive aquaculture in two nations: shrimp production in Thailand and salmon production in Norway. In both countries, aquaculture was shown to rely increasingly on imported subsidies. The rapid expansion of aquaculture turned these countries from fishmeal net exporters to fishmeal net importers, increasingly using inputs from the Southeastern Pacific Ocean.</p><p>As the examined agricultural and aquacultural production systems became globalized, levels of dependence on other nations’ ecosystems, the number of external supply sources, and the distance to these sources steadily increased. Dependence on other nations is not problematic, as long as we are able to acknowledge these links and sustainably manage resources both at home and abroad. However, ecosystem subsidies are seldom recognized or made explicit in national policy or economic accounts. Economic systems are generally not designed to receive feedbacks when the status of remote ecosystems changes, much less to respond in an ecologically sensitive manner. Papers IV and V discuss the problem of “masking” of the true environmental costs of production for trade. One of our conclusions is that, while the ArEAs approach is a useful tool for illuminating environmentally-based subsidies in the policy arena, it does not reflect all of the costs. Current agricultural and aquacultural production methods have generated substantial increases in production levels, but if policy continues to support the focus on yield and production increases alone, taking the work of ecosystems for granted, vulnerability can result. Thus, a challenge is to develop a set of complementary tools that can be used in economic accounting at national and international scales that address ecosystem support and performance.</p><p>We conclude that future resilience in food production systems will require more explicit links between consumers and the work of supporting ecosystems, locally and in other regions of the world, and that food security planning will require active management of the capacity of all involved ecosystems to sustain food production.</p>

De part son profil équilibré en acides aminés essentiels (AAE), la farine de poisson (FP) est la source protéique principale utilisée dans l’alimentation des crevettes d’élevage. Cependant, compte tenu des enjeux du développement durable de la production aquacole, son utilisation doit être réduite, et remplacée par d’autres sources protéiques comme les protéines végétales (PV), qui sont souvent carencées en lysine et méthionine mais riches en cystine. Les conséquences d’un tel changement alimentaire sont peu connues chez la crevette tigrée Penaeus monodon. Pour les évaluer, nous avons utilisédes aliments semi-purifiés reflétant les carences/excès en AA des PV pour estimer les besoins en protéine, lysine et méthionine pour l’entretien et la croissance. Tout en confirmant les données antérieures sur les besoins pour la croissance des stades postlarves, nous avons pu préciser la contribution de l’apport en ces deux acides aminés pour l’entretien. Au niveau métabolique, la variation de l’apport protéique (10, 30, 50% protéine brute) et la carence en méthionine (-30% par rapport au besoin) entraînent une modification de l’activité des enzymes du catabolisme des AA, mais pas celle des voies de reméthylation et transsulfuration. En revanche, et pour la première fois chez la crevette, nos résultats démontrent une épargne de la méthionine par la cystine (et la choline), soulignant l’importance de l’apport en AA soufrés totaux (methionine + cystine). Nos résultats illustrent aussi l’importance qu’il convient d’accorder à la disponibilité des AAE dans les études de remplacement de la FP par un mélange de PV pour améliorer l’utilisation azotée chez la crevette P. monodon. / Due to its well balanced essential amino acid (EAA) profile, fishmeal (FM) is the major protein source used in the formulation of aquafeed for cultured shrimp. To sustain farming systems, its incorporation, however, must be reduced and substituted by other protein sources less well nutritionally balanced, such as plant protein ( PP) which are often low in lysine and methionine but rich in cystine. The metabolic consequences of such a shift in dietary profile are not well known for the black tiger shrimp, Penaeus monodon. To describe these consequences, we used semi-purified diets limiting in lysine and methionine (to reflect PP profile) to determine juvenile requirements of protein, lysine and methionine for both maintenance and growth, applying a factorial approach. Our results confirm the previous data on growth requirement for post-larvalstages of P. monodon while also providing new data on maintenance requirements. At the metabolic level, a variation in the dietary protein level (10, 30, 50 % crude protein) and methionine (adequate or 30% lower) resulted in a significant change in the activity of transdeaminating enzyme, but not those of remethylation and transsulfuration. Nevertheless, we found for the first time that methionine utilisation for body protein accretion can be spared by cystine and choline (up to 50%) in this species, illustrating the importance to consider total sulphur AA supply. Our data also show that full consideration should be given to AA availability in order to develop practical diets with low FM levels for P. monodon.

Résumé Une demande croissante sans cesse de produits alimentaires à base d’animaux influence la productivité des systèmes mondiaux de production alimentaire, et des mesures indispensables pour freiner la dégradation de l’environnement promettent des effets similaires. Si les scénarios de demande future peuvent être satisfaits de manière durable, cela dépend notamment de la possibilité de réduire de manière significative l'impact de l'aquaculture et de l'élevage sur l'environnement. Des recherches récentes suggèrent que l'utilisation d'aliments à base d'insectes (IBF) pourrait apporter une contribution significative à cet égard, et des arguments valables sont avancés pour soutenir cette hypothèse. Les larves de mouches, comme celles des mouches domestiques (Musca domestica) ou des mouches soldat noir (Hermetia illucens), sont en mesure de puiser des nutriments dans un large éventail de ressources organiques, y compris celles impropres à la consommation humaine. Cela crée la possibilité de convertir (et de réduire considérablement) les déchets organiques de faible valeur, tels que le fumier ou le sang animal, en protéines de haute qualité et en énergie alimentaire, qui se sont avérés appropriés pour nourrir différents poissons d'aquaculture et du bétail monogastrique.Bien que le concept IBF promet d’importants avantages et ait démontré sa faisabilité technique, il n’existe encore aucun système établi permettant de tester les avantages supposés en termes de durabilité. Dans cette thèse, nous avons essayé de surmonter cette lacune par la modélisation de tels systèmes. Notre objectif central était d'identifier les aspects influençant le potentiel d'application des IBF dans différents contextes géographiques et de définir des voies d'optimisation pour une mise en œuvre durable. En nous basant sur des données expérimentales recueillies lors d'essais d'élevage menés en Europe (Espagne et Slovaquie) et en Afrique de l'Ouest (Ghana et Mali), nous avons formulé la conception d'un ensemble de versions de systèmes améliorés élevant M. domestica et H. illucens sur différents substrats organiques de faible valeur. Les modèles de production génériques ont servi comme base d’une analyse du cycle de vie ex ante, dans laquelle nous avons exploré les performances des systèmes à l’aide d’analyse du cycle de vie environnementale (ACV) et de l’analyse des coûts du cycle de vie (ACCV).Les ACVs et ACCVs ont montré que les performances environnementales et économiques des IBF sont largement fonction de l’efficacité de conversion des systèmes, de l’organisation du processus de production (c’est-à-dire de l’apport de main-d’œuvre et d’équipements technologiques) et du contexte géographique. La combinaison de ces facteurs a fourni des avantages pour les configurations simplistes utilisées dans la production de M. domestica en Afrique occidentale tropicale dans des conditions de ponte naturelle (c'est-à-dire d'inoculation de substrat par le biais de mouches présentes à l'état naturel). L'inoculation artificielle (c'est-à-dire l'inoculation du substrat par le biais de larves nourries provenant d'une colonie d'adultes en captivité), utilisée dans la production de H. illucens en Afrique de l'Ouest et de M. domestica dans le sud de l'Espagne, a favorisé une efficacité de conversion élevée, mais a augmenté les impacts environnementaux et les coûts, parce que le système complexe et l'organisation de processus à forte intensité de main-d'œuvre ont considérablement accru les intrants de main-d'œuvre et d'infrastructures de production.Une comparaison avec des aliments conventionnels riches en protéines a mis en évidence des inconvénients environnementaux et économiques pour les modèles de production actuels des IBF, notamment en ce qui concerne les aliments végétaux (par exemple, le tourteau de soja). Les disparités entre les alimentations IBF et conventionnelles reflètent l’utilisation des capacités sub-optimaux des systèmes (effet d’économie d’échelle insuffisant), ainsi que la perte d’énergie et de biomasse le long de la chaîne trophique (producteurs autotrophes vs consommateurs hétérotrophes). Les résultats soulèvent des doutes légitimes sur les avantages en termes de durabilité d’une mise en œuvre d'insectes dans les chaînes de valeur agricoles actuelles. Le succès commercial dépend en grande partie du niveau de salaire spécifique au contexte, des prix des substrats d'élevage et de la manière dont les marchés évaluent les multiples fonctions que les insectes sont capables de fournir. S'agissant de la performance environnementale, nos résultats nous amènent à conclure que la production d'IBF ne présente aucun avantage par rapport aux aliments conventionnels.L’évaluation de systèmes de production encore hypothétiques impliquait une bonne quantité d’hypothèses et d’approximations. Étant donné ces multiples sources d'incertitude et compte tenu du fait que seul un nombre limité de conceptions de systèmes possibles sont prises en compte, les déclarations sur le potentiel d'application d'IBF n'ont aucune validité universelle et doivent être interprétées avec prudence. Cependant, nos résultats contribuent à une meilleure compréhension des facteurs influant sur le potentiel d’application des systèmes de production d’insectes et constituent un point de référence précieux pour les discussions scientifiques et les activités de recherche et développement futures visant à mettre en place des modes de production alimentaire durables.Bien que nos recherches n’apportent aucun soutien aux avantages environnementaux ou économiques supposés de l’utilisation d’insectes dans l’alimentation animale, il est possible que leur utilisation comme aliment destiné à la consommation humaine directe (c’est-à-dire comme substitut possible aux produits à base de poisson et de viande) constitue une solution durable aux problèmes actuels et futurs. Nous recommandons donc aux recherches futures de se concentrer sur les techniques permettant d'exploiter les insectes comme nourriture. / Doctorat en Sciences / There are a few details that I could not specify in the available input fields. I would like to ask you kindly to add the following information: (1) Prof. Erik Mathijs (KUL) is my second co-supervisor; (2) Next to the Jury members listed, there are Prof. Matthias Finkbeiner (TU Berlin) and Prof. Theo Niewold (KU Leuven), which I could not enter manually. / info:eu-repo/semantics/nonPublished

Modern food production is a complex, globalized system in which what we eat and how it is produced are increasingly disconnected. This thesis examines some of the ways in which global trade has changed the mix of inputs to food and feed, and how this affects food security and our perceptions of sustainability. One useful indicator of the ecological impact of trade in food and feed products is the Appropriated Ecosystem Areas (ArEAs), which estimates the terrestrial and aquatic areas needed to produce all the inputs to particular products. The method is introduced in Paper I and used to calculate and track changes in imported subsidies to Swedish agriculture over the period 1962-1994. In 1994, Swedish consumers needed agricultural areas outside their national borders to satisfy more than a third of their food consumption needs. The method is then applied to Swedish meat production in Paper II to show that the term “Made in Sweden” is often a misnomer. In 1999, almost 80% of manufactured feed for Swedish pigs, cattle and chickens was dependent on imported inputs, mainly from Europe, Southeast Asia and South America. Paper III examines ecosystem subsidies to intensive aquaculture in two nations: shrimp production in Thailand and salmon production in Norway. In both countries, aquaculture was shown to rely increasingly on imported subsidies. The rapid expansion of aquaculture turned these countries from fishmeal net exporters to fishmeal net importers, increasingly using inputs from the Southeastern Pacific Ocean. As the examined agricultural and aquacultural production systems became globalized, levels of dependence on other nations’ ecosystems, the number of external supply sources, and the distance to these sources steadily increased. Dependence on other nations is not problematic, as long as we are able to acknowledge these links and sustainably manage resources both at home and abroad. However, ecosystem subsidies are seldom recognized or made explicit in national policy or economic accounts. Economic systems are generally not designed to receive feedbacks when the status of remote ecosystems changes, much less to respond in an ecologically sensitive manner. Papers IV and V discuss the problem of “masking” of the true environmental costs of production for trade. One of our conclusions is that, while the ArEAs approach is a useful tool for illuminating environmentally-based subsidies in the policy arena, it does not reflect all of the costs. Current agricultural and aquacultural production methods have generated substantial increases in production levels, but if policy continues to support the focus on yield and production increases alone, taking the work of ecosystems for granted, vulnerability can result. Thus, a challenge is to develop a set of complementary tools that can be used in economic accounting at national and international scales that address ecosystem support and performance. We conclude that future resilience in food production systems will require more explicit links between consumers and the work of supporting ecosystems, locally and in other regions of the world, and that food security planning will require active management of the capacity of all involved ecosystems to sustain food production.

En Nouvelle-Calédonie il y a une forte volonté politique pour diversifier l'aquaculture qui repose encore aujourd'hui sur la crevetticulture. Dans ce contexte le crabe de palétuvier est considéré comme une espèce à fort potentiel. Un des principaux verrous au développement de la carcinoculture en Nouvelle-Calédonie est la disponibilité d'un aliment granulé commercial. Ainsi le principal objectif de cette thèse est d'améliorer notre connaissance des besoins nutritionnels du crabe de palétuvier afin d'être en mesure de formuler un aliment équilibré pour son élevage. Cependant avant d'aborder les études nutritionnelles nous avons vérifié le nombre d'espèces de crabes de palétuvier présentes en Nouvelle-Calédonie.Nos résultats d'études morphologiques et génétiques de 63 individus provenant de 9 sites des côtes Ouest et Nord-Est de la Nouvelle-Calédonie ont confirmé l'existence d'une unique espèce commercialisée: Scylla serrata. C'est donc sur cette espèce que nous avons travaillé en nutrition avec deux séries expérimentales ayant pour objectifs: i) d'évaluer le concentré protéique de soja (CPS) en comparaison avec la farine de poisson comme principale source en protéines et ii) de déterminer le taux optimum d'incorporation du CPS pour la mue et la croissance tissulaire des animaux. Nous avons ainsi observé deux phases de croissance tissulaire au cours d'un cycle de mue (CM): une phase rapide (CTR) qui démarre après la mue et dure jusqu'au début de l'intermue (elle représente 30% du CM) suivi d'une phase de croissance lente (CTL) sur toute la durée de l'intermue et jusqu'à la mue suivante (elle représente 70% du CM). L'accumulation des protéines et des lipides au cours du CM a suivi le même profil de croissance tissulaire contrairement aux cendres qui ont augmenté de façon rapide durant 5 jours suivant l'ecdysis pour atteindre un plateau jusqu'à la prochaine mue. Les deux phases de croissance étaient corrélées avec une prise de l'aliment par les animaux maximale pendant les deux premières semaines suivant la mue. Elle a diminué de moitié sur les 5 semaines suivantes et s'est maintenue ensuite à un niveau de base jusqu'à la prochaine mue. L'énergie ingérée était allouée principalement à la croissance et à l'entretient respectivement durant les périodes CTR et CTL. Durant la phase de croissance lente, 28% de l'énergie ingérée étaient mise en réserve en prévision de la prochaine mue. Le remplacement de la farine de poisson par la CPS n'a pas modifié la croissance tissulaire,l'efficience de l'aliment et le bilan énergétique des animaux quelque soit la phase de croissance considérée. Le taux d'incorporation dans l'aliment de 42% de CPS a permis la meilleure croissance (fréquence de mue et croissance tissulaire, efficience de l'aliment et la rétention de l'énergie des protéines et des lipides. L'hypothèse d'une toxicité de l'ammonium issu de la dégradation des protéines en excès ou des facteurs antinutritionnels du soja est avancée pour expliquer les effets négatifs observés avec les aliments renfermant des taux d'incorporation élevés en CPS. En conclusion, nos travaux apportent des informations originales sur la croissance tissulaire et les dépenses énergétiques durant un cycle de mue et la capacité du crabe juvénile d'utiliser le CPS comme principale source de protéines. Sur ces bases nous somme en mesure de préconiser des contraintes nutritionnelles permettant de formuler un aliment équilibré sans farine de poissons pour l'élevage du crabe de palétuvier S.serrata. / In New Caledonia, there is the strong political will to diversify aquaculture which is mainly based on shrimp farming. In this context, mud crabs have been considered as a potential species for aquaculture development. One of the main constraints to develop crab farming is the availability of formulated feed. Thus, the main purpose of this thesis is to get information on the crab nutritional requirements in order to formulate a balanced diet. However, we had to clarify first how many species of mud crab were present in New-Caledonia. The result of our morphological and genetic investigations carried out on 63 specimens from 9 areas of the west and northeast coast of New-Caledonia confirmed that only one species, Scylla serrata, is commercialized in this country. Consequently, S. serrata was used in our nutritional study based on two experiments to: i) evaluate the soy protein concentrate (SPC) compared with the fishmeal as the main protein source and ii) determine the optimum level of SPC in the diet for molting and tissue growth. We observed two tissue growth phases within one molt cycle (MC): a fast tissue growth (FTG) occurred after ecdysis until early intermolt stage (30% of MC) which is followed by a slow tissue growth (STG) period from intermolt to ecdysis (70% of MC). Protein and lipid deposition followed the same trend than tissue growth while ash level increased quickly during five days after molt and then remained stable until the next molt. The two growth phases were correlated with the voluntary feed intakes (VFI) which was maximum during 2 weeks after ecdysis and then decreased by 50% over the five following weeks to reach a baseline until the next molt. Intake energy was allocated mainly for growth during FTG period and for maintenance during STG period. During STG, 28% of the ingested energy was accumulated for the next ecdysis. Replacement of fishmeal by SPC as main protein source did not affect tissue growth, efficiency of feed utilization and energy budget of crabs whatever the tissue growth period considered. The dietary SPC inclusion of 42% in the diet promoted growth (molt frequency and tissue growth), feed efficiency and retention of energy, protein and lipid. Hypothesis related to ammonia toxicity from catabolism of proteins in excess or anti-nutritional factors from soybean could explain the negative effects of higher inclusion of SPC in the diet for juvenile crabs. In conclusion, our work brings novel information on tissue growth, energy budget during a molt cycle and the ability of juvenile crab to use SPC as a main source of protein. On this basis we suggest to formulate nutritionally balanced diet without fishmeal to farm juvenile mud crabs S. serrata.

[EN] The present study was designed to evaluate in independent trials the effect of replace protein fishmeal (HP) by four plant sources, lupine (Lupinus mutabilis Sweet), corn gluten (CGM), amaranth (Amaranthus caudatus L.) and quinoa (Chenopodium quinoa) on the growth of juvenile shrimp Litopenaeus vannamei. For this four sets of diets were developed. The first two containing 35% protein and 11% lipids were prepared to replace the 0, 25, 50, 75 and 100% of the protein from HP by protein lupine (LKM) or CGM. The other two series of isoproteic (30%) and isolipidic (9.5%) diets were formulated to replace 0, 15, 25, 35 and 45% protein HP by protein amaranth and quinoa. Only the contents of corn starch and fish oil were varied to maintain constant levels of protein and lipid in all the experimental diets. All diets had squid meal to provide attractability. Depending on the test conducted eight (LKM and CGM) or seven (amaranth and quinoa) juveniles of about 1g were stocked randomly in aquariums (44 m-2 or 39 m-2) 50 L equipped with a flow-through water system using full-strength seawater. Six aquariums (replicates) were assigned to each of the treatments in a completely randomized design. The shrimp were fed ad libitum twice a day for about eight weeks. At the end of the growth trial, shrimp were fed experimental diets containing 0.5% of chromium oxide. Overall survival in the study was higher than 74% and did not change significantly (p>0.05) when HP was replaced partially or completely by each of the sources evaluated. The results of this study showed that LKM can replace 50% of the protein of HP without significantly (p>0.05) reducing growth (6.7-7.0 g final weight). LKM inclusion in any of the tested levels resulted in a significantly (p<0.05) reduced the apparent digestibility of dry matter (ADMS) and apparent digestibility of crude protein (ADPC) of the feed. The gradual increase of CGM in diets produced a significant (p<0.05) decrease of shrimp final weight (5.9 to 3.2 g) and growth rate (2.7 to 1.7% d-1) compared to those fed CGM 0 (7.1 g and 3.0% d-1). Feed Conversion Factor (FCA) was also significantly (p<0.05) affected by CGM level, diets CGM50, CGM75 and CGM100 had higher FCA than CGM0 and CGM25. The inclusion of CGM on any level tested resulted in a significant decrease in ADMS, from 77.9 to 66.0%, and ADPC, 80.5 to 52.0%, of the feed. The apparent digestibility of amino acids, except lysine, declined with the addition of CGM, reflecting the ADPC. While those shrimps fed diets based on amaranth showed that the diet with 15% replacement obtained a better growth (p<0.05) after the control diet. Diets with replacement 15% and 25% reported significantly (p<0.05) better DAMS (79.7% and 71.2%) and ADPC (88.4% and 81.1%) than the diet with 35% and 45% substitution. The replacement of quinoa in any of the assessed levels have not demonstrated performance (p<0.05) lower than the control diet. The DAMS and ADPC for quinoa diets were statistically superior (p<0.05) than control diet. These results show that lupine and quinoa have a very good potential as a protein source up to 50% and 45% respectively of the HP protein which is equivalent to a third of the total protein in the diet. The cost-benefit of including these ingredients needs to be evaluated. Lower values of corn gluten and amaranth on the HP could be due to low protein digestibility, imbalance of amino acids and / or the presence of antinutritional factors. / [ES] El presente estudio fue diseñado para evaluar en ensayos independientes el efecto de reemplazar la proteína de la harina de pescado (HP) por cuatro fuentes de origen vegetal, altramuz (Lupinus mutabilis Sweet), gluten de maíz (CGM), amaranto (Amaranthus caudatus L.) y quinua (Chenopodium quinoa) sobre el crecimiento de camarones juveniles Litopenaeus vannamei. Para esto se elaboraron cuatro series de dietas. Las dos primeras conteniendo 35% de proteína y 11% de lípidos fueron preparadas para sustituir el 0, 25, 50, 75 y 100% de la proteína proveniente de la HP por proteína de las harinas de altramuz (LKM) o CGM. Las otras dos series de dietas isoproteicas (30%) e isolipidicas (9,5%) fueron formuladas para reemplazar 0, 15, 25, 35 y 45% de la proteína de la HP por proteína de amaranto y quinua. Solamente los contenidos de almidón de maíz y aceite de pescado variaron para mantener constante los niveles de proteína y lípidos en todas las dietas experimentales. Todas las dietas tuvieron harina de calamar. Dependiendo del ensayo realizado ocho (LKM y CGM) o siete (amaranto y quinua) juveniles de alrededor de 1g fueron sembrados aleatoriamente en los acuarios (44 m-2 o 39 m-2) de 50 l equipados con un sistema de recambio de agua de mar de flujo continuo. Seis acuarios (réplicas) fueron asignadas a cada uno de los tratamientos en un diseño completamente aleatorizado. Los camarones fueron alimentados ad libitum dos veces al día por aproximadamente ocho semanas. Al final del ensayo de crecimiento, los camarones fueron alimentados con las dietas experimentales conteniendo 0,5% de óxido de cromo. La supervivencia en general del estudio fue superior a 74% y no varió significativamente (p>0,05) cuando la HP fue reemplazada parcial o totalmente por cada una de las fuentes evaluadas. Los resultados de este estudio mostraron que LKM puede reemplazar el 50% de la proteína de la HP sin disminuir significativamente (p>0,05) el crecimiento (6,7-7,0 g peso final). La inclusión de LKM en cualquiera de los niveles ensayados resultaron en una significativamente (p<0,05) reducción de la digestibilidad aparente de materia seca (DAMS) y la digestibilidad aparente de proteína cruda (DAPC) del alimento. El gradual incremento del CGM en las dietas produjo un significativo (p<0,05) decrecimiento del peso final (5,9 a 3,2 g) de los camarones y sus tasas de crecimiento (2,7 a 1,7% d-1) comparado a aquellos alimentados con 0 CGM (7,1 g y 3,0 % d-1). El Factor de Conversión Alimenticia (FCA) fue también significativamente (p<0,05) afectado por el nivel de CGM, las dietas CGM50, CGM75 y CGM100 tuvieron un más alto FCA que CGM0 y CGM25. La inclusión de CGM en cualquier nivel ensayado resultó en un significativo decrecimiento en DAMS, de 77,9 a 66,0%, y en DAPC, de 80,5 a 52,0%, del alimento. La digestibilidad aparente de aminoácidos, con la excepción de lisina, declinó con la incorporación de CGM, reflejando la ADPC. En tanto que los camarones alimentados con las dietas a base de amaranto mostraron (p<0,05) que la dieta con 15% de reemplazo obtuvo un mejor crecimiento después de la dieta control. Las dietas con reemplazo de 15% y 25% registraron significativamente (p<0,05) una mejor DAMS (79,70% y 71,21%) y DAPC (88,39% y 81,10%) que las dieta con 35% y 45% de sustitución. El reemplazo de la quinua en cualquiera de los niveles evaluados no demostraron tener un rendimiento (p<0,05) inferior a la dieta control. La DAMS y DAPC para las dietas con quinua fueron estadísticamente superiores (p<0,05) a la dieta control. Estos resultados muestran que el altramuz y quinua tienen un potencial muy bueno como fuente proteína hasta el 50% y 45% respectivamente de la proteína de la HP lo cual es equivalente a un tercio del total de la proteína presente en la dieta. Los valores más bajos del gluten de maíz y amaranto relativo a la HP podrían ser debido a la baja digestibilidad de la proteína, imbalance de aminoácidos y/o a la pres / [CAT] El present estudi va ser dissenyat per a avaluar en assajos independents l'efecte de reemplaçar la proteïna de la farina de peix (HP) per quatre fonts d'origen vegetal, tramús (Lupinus mutabilis Sweet), gluten de dacsa (CGM), amarant (Amaranthus caudatus L.) i quinoa (Chenopodium quinoa) sobre el creixement de gambetes juvenils Litopenaeus vannamei. Per a açò es van elaborar quatre sèries de dietes. Les dos primeres contenint 35% de proteïna i 11% de lípids van ser preparades per a substituir el 0, 25, 50, 75 i 100% de la proteïna provinent de la HP per proteïna de les farines de tramús (LKM) o CGM. Les altres dos sèries de dietes isoproteicas (30%) i isolipidicas (9,5%) van ser formulades per a reemplaçar 0, 15, 25, 35 i 45% de la proteïna de la HP per proteïna d'amarant i quinoa. Només els continguts de midó de dacsa i oli de peix van variar per a mantindre constant els nivells de proteïna i lípids en totes les dietes experimentals. Totes les dietes van tindre farina de calamar per a proveir atractabilidad. Depenent de l'assaig realitzat huit (LKM i CGM) o set (amarant i quinoa) juvenils d'al voltant de 1g van ser sembrats aleatòriament en els aquaris (44 m-2 o 39 m-2) de 50 l equipats amb un sistema de recanvi d'aigua de mar de flux continu. Sis aquaris (rèpliques) van ser assignades a cada un dels tractaments en un disseny completament aleatorizado. Les gambetes van ser alimentats ad libitum dos vegades al dia per aproximadament huit setmanes. Al final de l'assaig de creixement, les gambetes van ser alimentats amb les dietes experimentals contenint 0,5% d'òxid de crom. La supervivència en general de l'estudi va ser superior a 74% i no va variar significativament (p>0,05) quan la HP va ser reemplaçada parcial o totalment per cada una de les fonts avaluades. Els resultats d'este estudi van mostrar que LKM pot reemplaçar el 50% de la proteïna de la HP sense disminuir significativament (p>0,05) el creixement (6,7-7,0 g pes final). La inclusió de LKM en qualsevol dels nivells assajats van resultar en una significativament (p<0.05) reducció de la digestibilitat aparent de matèria seca (DAMS) i la digestibilitat aparent de proteïna crua (DAPC) de l'aliment. El gradual increment del CGM en les dietes va produir un significatiu decreixement del pes final (5,9 a 3,2 g) de les gambetes i les seues taxes de creixement (2,7 a 1,7% d-1) comparat a aquells alimentats amb 0 CGM (7,1 g i 3,0 % d-1). El Factor de Conversió Alimentària (FCA) va ser també significativament (p<0.05) afectat pel nivell de CGM, les dietes CGM50, CGM75 i CGM100 van tindre un més alt FCA que CGM0 i CGM25. La inclusió de CGM en qualsevol nivell assajat va resultar en un significatiu decreixement en DAMS, de 77,9 a 66,0%, i en DAPC, de 80,5 a 52,0%, de l'aliment. La digestibilitat aparent d'aminoàcids, amb l'excepció de lisina, va declinar amb la incorporació de CGM, reflectint l'ADPC. En tant que les gambetes alimentats amb les dietes a base d'amarant van mostrar que la dieta amb 15% de reemplaçament va obtindre un millor creixement després de la dieta control. Les dietes amb reemplaçament de 15% i 25% van registrar significativament una millor DAMS (79,70% i 71,21%) i DAPC (88,39% i 81,10%) que les dieta amb 35% i 45% de substitució. El reemplaçament de la quinoa en qualsevol dels nivells avaluats no van demostrar tindre un rendiment inferior a la dieta control. La DAMS i DAPC per a les dietes amb quinoa van ser estadísticament superiors a la dieta control. Estos resultats mostren que el tramús i quinoa tenen un potencial molt bo com a font proteïna fins al 50% i 45% respectivament de la proteïna de la HP la qual cosa és equivalent a un terç del total de la proteïna present en la dieta. El cost-benefici d'incloure estos ingredients necessita ser avaluat. Els valors més baixos del gluten de dacsa i amarant relatiu a la HP podrien ser degut a la baixa digestibilitat de la proteïna, imbalance d'aminoàcids y/o a / Molina Poveda, C. (2016). Evaluación de varias fuentes de proteína vegetal en dietas para camarón Litopenaeus vannamei [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/63666 / TESIS

La harina de pescado es uno de los commodities que lidera las exportaciones del Perú, seguido de países como Dinamarca, Chile, Estados Unidos, Vietnam, entre otros. Dentro los años 2015 al 2019, el incremento de las exportaciones de harina de pescado hacia China ha sido muy relevante, esto se debe a que este producto es una de las fuentes proteicas más importantes a nivel mundial para la alimentación ganadera, por lo que ha tenido una tendencia muy positiva. El objetivo de la investigación es determinar la relación entre los procesos logísticos y las exportaciones de la harina de pescado dentro de este periodo. Se optó por realizar una investigación cuantitativa. En ella, se tomó la decisión de usar como herramienta a la encuesta, con este instrumento se procedió a encuestar a 30 colaboradores con la experiencia necesaria en el rubro de las principales empresas exportadoras de harina de pescado. Asimismo, para realizar el análisis de las respuestas brindadas, se utilizó el programa estadístico SPSS, con el objetivo de poder evaluar las hipótesis que fueron planteadas. De esta forma, se pudo determinar que los procesos logísticos si tienen relación con las exportaciones de harina de pescado como la gestión de inventarios, almacenamiento, compras y transporte. / Fishmeal is one of the commodities that leads Peru's exports, followed by countries such as Denmark, Chile, the United States, Vietnam and others. Within the years 2015 to 2019, the increase in fishmeal exports to China has been very relevant, this is due to the fact that this product is one of the most important protein sources worldwide for livestock feed, so it has had a very positive trend. The objective of the research is to determine the relationship between logistics processes and fishmeal exports within this period, it was decided to carry out a quantitative research, in which the decision was made to use the survey as a tool, with this instrument 30 employees with the necessary experience in the field were surveyed, from the main fishmeal exporters, the leading companies in the market. For this reason, to perform the analysis of the answers provided, the statistical program SPSS was used, in order to be able to evaluate the hypotheses that were raised. In this way, it was possible to determine that the logistics processes are related to fishmeal exports such as inventory management, storage, purchases and transportation. / Tesis

The Peruvian anchoveta fishery was for nearly 60 years characterized by the unsustainable dynamics of open access resource pools. This thesis investigates whether the 2009 Peruvian Legislative Decree 1084 on Individual Vessel Quotas (IVQ's) is an effective response to the industrial overcapacity and race-to-fish problems that threatened the environment before its implementation. It employs Common-Pool Resource theory to assess the impact of the new IVQ scheme on collective dynamics, and Ribot and Peluso's access theory (2009) to elucidate evolving power relations in the fishery. In this framework, DL1084 is evaluated as a regulatory instrument, as a lens on fisheries governance in Peru, and as a source of insight into how environmental impact serves in developing regulations of natural resource exploitation. A triangulated mixed-method design is employed: (1) a two-stream literature review of fisheries management and of the fishery's political ecology; (2) a quantitative analysis of daily state-published landings reports; and (3) seven in-depth intensive interviews with key actors in the fishery, conducted in Peru over two field-trips of approximately 2 months in total. Results indicate that although IVQ's supported existing trends towards large-scale economic efficiency and altered extreme competitive strategies previously associated with open access, fishing companies have built larger ships, favour bigger catches and still concentrate fishing effort around a given annual peak. Meanwhile, capacity has again increased in the unregulated artisanal fishery sector. DL1084 appears to reflect a broader process of growing private sector involvement in managing the fishery, made official by its institutionalization of market self-regulation. Ultimately, it evidences deep structure and capacity limitations in the state's ability to govern marine resources. The law was nevertheless seen as a landmark for the environmental legal process in Peru and an opportunity for reform. This thesis suggests that studying such legislations can provide insight into state identity and the evolving relationship between a nation and its geography. / Durante casi 60 años, la pesquería peruana de anchoveta estaba caracterizada por las dinámicas insostenibles de la explotación de recursos de propiedad común en situación de acceso abierto. Esta tesis investiga si el Decreto Legislativo Peruano 1084 (DL1084) sobre Límites Máximos de Captura por Embarcación (LMCE) es una respuesta efectiva a los problemas de sobrecapacidad industrial y de carrera por el recurso que amenazaban el ambiente antes de su aplicación en 2009. Se basa en la teoría de los Recursos de Propiedad Común (Common-Pool Resource theory) para evaluar el impacto del nuevo modelo de gestión por LMCE sobre las dinámicas colectivas, y en la teoría del Acceso de Ribot y Peluso (2009) para trazar la evolución de las relaciones de poder en la pesquería. En este marco, el DL1084 es evaluado como herramienta regulatoria, como lente sobre la gobernanza pesquera en el Perú y como reflejo del proceso por el cual el impacto ambiental sirve como base para el desarrollo de regulaciones sobre el acceso a recursos naturales. Sigue un diseño triangulado de métodos combinados: (1) una revisión de literatura en dos ramas de la gestión de pesquería como campo general y de la ecología política de la pesquería de anchoveta; (2) un análisis cuantitativo de los informes diarios de desembarques publicados por el estado (IMARPE); y (3) siete entrevistas intensivas de fondo con actores claves en la pesquería, realizadas en Perú durante dos visitas de aproximadamente dos meses en total. Los resultados indican que a pesar de que los LMCE soportaron una tendencia existente hacia una eficiencia económica de mayor escala y alteraron las estrategias competitivas extremas asociadas con el acceso abierto, las empresas pesqueras han construido embarcaciones más grandes, favorecen capturas de mayor tamaño y todavía concentran su esfuerzo pesquero alrededor de un pico anual de abundancia. En paralelo, ha aumentado la capacidad de captura en el sector artesanal, el cual no cuenta con límites de captura. El DL1084 aparece como parte de un proceso más general de creciente involucramiento del sector privado en el manejo de la pesquería, haciéndolo oficial por su misma institucionalización de la autorregulación del mercado. Por último, pone en evidencia profundas limitaciones de estructura y de capacidad en el estado en cuanto a cómo gobierna los recursos marinos. La ley ha sin embargo sido vista como un precedente importante para el proceso legislativo ambiental en el Perú así como una oportunidad para impulsar otras reformas. Esta tesis sugiere que el estudio de tales legislaciones puede ofrecer una mirada sobre los procesos de formación de la identidad de un estado y sobre la evolución de la relación entre una nación y su geografía.