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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
191

Project Management Competence and Complexity in Projects : Impact study of performance of mega engineering projects in Pakistan

Usman, Muhammad January 2018 (has links)
The aim of this research is to test the effect of different project complexities and project management competence of project managers in public sector mega engineering projects of Pakistan and their effect on project performance. This research study is applied in Pakistan. Complexities in a project are inevitable. These cannot be controlled or eliminated but one can have their better understanding to counter them resulting in better project performance.  Quantitative research techniques have been applied for this particular research study. A total of 100 questionnaires were handed out among respondents, of which 82 survey questionnaires were useful for further analysis. Hypotheses have been developed to test the effect of variables of  project management competence on project performance and subsequently the effect of Technical, Organizational and environmental complexity on project performance.   Results show that project management competence have a positive effect on project performance, like leadership, management skills, communication, ethics, honesty have a strong impact on project performance. Secondly, Technical and organizational complexity have a negative effect on project performance whereas hypothesis of environmental complexity has not supported the project performance.  Further, a number of complexities were high ranked. For instance, goals, tasks and market conditions were ranked very high with respect to their impact on project performance.  This study contributes theoretically in the field of project management and on practical level study will not only help the Practioners in the field of PM, but also helpful for project managers of mega engineering projects. On societal level this study may help the Government intuitions in formulating rules and regulations such as PM certifications that can improve the project performance.
192

Stora sportevenemang i mindre städer : En kvantitativ studie om platsmarknadsföring och kunskap-attityd hos invånare vid större sportevenemang

Eklöf, Rebeca, Jansson, Elin January 2018 (has links)
Cities hosting huge sporting events is a growing industry and is a way to market the city towards the rest of the world. The goal is for businesses to make profit, attract tourist and be seen on the global market. However, as its marketed towards external audiences, the locals and how they are affected are usually forgotten in the process. The purpose of this paper was to study the knowledge and what attitudes individuals living in Halmstad, Sweden, possess regarding the World Table Tennis Championship 2018, as well as how they received information regarding the event. To find the answers to our questions we handed out surveys in central Halmstad during the period the event took place. We compared our results with how they're tied to theories regarding place branding, image-identity-reputation and the knowledge-attitude-behavior model. The main findings of our analysis were that our respondents of Halmstad county was well- informed about the event and did not feel like they needed more information. The majority viewed the event in a positive light, and overall people found the event to be important for Halmstad as a city and destination.
193

A cobertura da mídia impressa na Copa das Confederações 2013 e Copa do Mundo 2014: a liberdade de expressão nas manifestações sociais / The print media coverage in the 2013 Confederations Cup and World Cup 2014: freedom of expression in social events

Belan, Bárbara Bressan [UNESP] 11 August 2016 (has links)
Submitted by Bárbara Bressan Belan null (ba_92_b@hotmail.com) on 2016-09-19T14:44:32Z No. of bitstreams: 1 DEFESA MESTRADO BÁRBARA_AUTOARQUIVAMENTO.pdf: 2487772 bytes, checksum: d8af82cf6dce1283b21db1c295660e2a (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-09-22T13:46:30Z (GMT) No. of bitstreams: 1 belan_bb_me_bauru.pdf: 2487772 bytes, checksum: d8af82cf6dce1283b21db1c295660e2a (MD5) / Made available in DSpace on 2016-09-22T13:46:30Z (GMT). No. of bitstreams: 1 belan_bb_me_bauru.pdf: 2487772 bytes, checksum: d8af82cf6dce1283b21db1c295660e2a (MD5) Previous issue date: 2016-08-11 / O direito à liberdade de expressão é garantido pela Constituição brasileira de 1988, e pode ser exercido de diversas formas. Entre junho de 2013 e julho de 2014, esse direito foi exercido por milhões de brasileiros que foram às ruas protestar contra a precariedade da saúde e educação pública, a corrupção, o aumento da tarifa de ônibus e também contra os gastos excessivos para a realização da Copa do Mundo. As manifestações sociais aconteceram na mesma época em que dois megaeventos esportivos eram realizados no país: a Copa das Confederações, em 2013, e a Copa do Mundo, em 2014. O objetivo desta pesquisa é verificar se dois veículos de comunicação impressos, Folha de S. Paulo e O Globo, que noticiaram as manifestações sociais, trataram-nas como um exercício pleno do direito à liberdade de expressão, ou criminalizaram o movimento. Para cumprir os objetivos, será utilizada a metodologia da análise de conteúdo. A hipótese é de que os jornais abordaram o assunto de maneira superficial e negativa. A partir das análises quantitativas e qualitativas realizadas com base na metodologia escolhida, tornou-se possível a confirmação de tal hipótese. / The right to freedom of expression is guaranteed by the Brazilian Constitution of 1988, and can be practiced by many different ways. Between june of 2013 and july of 2014, this right was practiced by millions of Brazilians, that went to the street to protest against the precariousness of public health and education, corruption, the increase in the bus pass and also against excessive expenses for the realization of the World Cup. The social manifestations happened in the same time that two sportive mega events were realized in the country: The Confederations Cup, in 2013, and The World Cup, in 2014. The object of this research is to verify if two newspapers, Folha de S. Paulo and O Globo, that noticed the social manifestations, treated them as an exercise of the right to freedom of expression, or criminalized the protest. To meet the goals, it will be used the methodology of content analysis. The hypothesis is that the newspapers dealt with the issue in a superficial and negative way. By the quantitative and qualitative analysis based on the chosen method, it became possible to confirm this.
194

Santa Catarina e os megaeventos esportivos : um estudo a partir da paradiplomacia / Santa Catarina and Sports Mega-events: a study from paradiplomacy

Meirinho, André Furlan 09 February 2015 (has links)
Made available in DSpace on 2016-12-08T16:55:45Z (GMT). No. of bitstreams: 1 123322.pdf: 6933733 bytes, checksum: c8b240c5509066e5772dbb7d7cdfb5c5 (MD5) Previous issue date: 2015-02-09 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Brazil is hosting major global sporting events like the 2014 FIFA World Cup Brazil and the Olympic and Paralympic Games Rio 2016, known as sports mega-events. When performing these competitions are presented several opportunities and threats involved, with positives and negatives points of view. Santa Catarina has the opportunity to be involved and act on these events and others like the Volvo Ocean Race and the FIA Formula One Grand Prix, receiving competitions or delegations from countries from different continents and several sports. The aim is to study of sports mega-events, particularly in the spot of the paradiplomacy (international activities of subnational actors such as the State and / or municipalities), for understanding possibilities of action in partnership with international actors and nations. The objective is to analyse the projection of the state and municipalities of Santa Catarina before, during and after the competitions, and how the sports megaevents can promote tourism development and encourage strategic partnerships. To this end, it discusses the relation of sports mega-events to politics and international relations, and possibilities of international projection of nations through soft power. The aim is to also highlight Santa Catarina and cities involved in the sports megaevents and analyse interviews with state managers, municipalities and other actors in Santa Catarina related to sports mega-events as to which objectives seeks to achieve, what image that seeks to project, about how is the international network, which are the priority countries and degree of synergy among stakeholders. Finally, it is still reviewing the possibilities for international partnerships, the importance of public management for results, as well as the focus on tourism projection. / O Brasil está sediando os maiores eventos esportivos globais como a Copa do Mundo da FIFA Brasil 2014 e os Jogos Olímpicos e Paralímpicos Rio 2016, conhecidos como megaeventos esportivos. Ao realizar estas competições se apresentam várias oportunidades e ameaças para os envolvidos, com pontos positivos e negativos. Santa Catarina tem a possibilidade de estar envolvida e atuar nestes eventos e em outros como a Volvo Ocean Race e o GP da FIA de Fórmula 1, recebendo as competições ou as delegações de países de diversos continentes e várias modalidades esportivas. Busca-se assim realizar um estudo dos megaeventos esportivos, particularmente à luz da paradiplomacia (atuação internacional de atores subnacionais como o Estado e/ou municípios), para a compreensão de possibilidades de atuação em parceria com os atores internacionais e nações. Objetiva-se com isso analisar a projeção do Estado e de municípios catarinenses antes, durante e depois das competições, e de que forma os megaeventos esportivos podem favorecer o desenvolvimento do turismo e estimular parcerias estratégicas. Para este fim, problematiza-se a relação dos megaeventos esportivos com a política e as relações internacionais, e as possibilidades de projeção internacional das nações através do soft power. Busca-se também destacar Santa Catarina e as cidades envolvidas com os megaeventos esportivos e analisa-se entrevistas com gestores do Estado, municípios e demais atores catarinenses relacionados aos megaeventos esportivos quanto a quais objetivos busca-se alcançar, qual imagem que se busca projetar, sobre como se dá a articulação internacional, quais países são prioritários e o grau de sinergia entre os envolvidos. Por fim analisa-se ainda as possibilidades de parcerias internacionais, a importância da gestão pública para resultados, bem como o foco na projeção do turismo.
195

Megaeventos e direito à moradia: como opera o empreendedorismo urbano no contexto do neodesenvolvimentismo. O caso do Parque Olímpico no Rio de Janeiro / Mega-events and the right to housing: how the urban entrepreneurialism operates in the context of neo-developmentism. The case of the Olympic Park in Rio de Janeiro

Mariana Gomes Peixoto Medeiros 23 September 2014 (has links)
Esta pesquisa busca investigar um processo em curso, onde a estratégia do empreendedorismo na gestão das políticas públicas urbanas vem se adaptando e se conformando ao atual contexto de política econômica chamada de neodesenvolvimentismo. Para isso, as parcerias público-privadas tem um papel fundamental na gestão do desenvolvimento urbano, no âmbito das três esferas de poder. Abordaremos, portanto, o histórico das reformas políticas do País que levaram a uma evolução da política de nacional-desenvolvimentismo ao novo desenvolvimentismo, para entendermos o contexto no qual as atuais parcerias público-privadas vem sendo desenvolvidas como modelo para de gerir o território. Mencionaremos também a legislação e os discursos por trás da defesa deste tipo de negócio entre o poder público e o setor privado. Ainda, como o país enfrentou a crise de 2008, chamada de crise das crises, utilizando esse modelo de gestão do território. Os megaeventos esportivos serão analisados como um fator catalizante da atração de investimentos e negócios para as cidades. No Rio de Janeiro, com a escolha da cidade para sediar os Jogos Olímpicos, e também sendo uma das cidades sedes da Copa do Mundo FIFA de 2014, várias de transformações territoriais se iniciaram. Com fortes indícios de que uma série de violações estava por vir, a fim de abrir espaço e ampliar o mercado dos investimentos privados. O Parque Olímpico, como principal local de competições das Olimpíadas de 2016, configura-se como uma imensa oportunidade de negócios com a iniciativa privada, localizado em área de ampla expansão do mercado imobiliário da cidade: a Barra da Tijuca. Diante disso, muitas comunidades pobres vem sofrendo com a política de deslocamento forçado para dar lugar aos investimentos no território. Neste contexto, está a comunidade Vila Autódromo, estabelecida bem ao lado do local de implementação do empreendimento. Sua remoção está prevista no estudo de viabilidade e no edital de concessão. Estudaremos a parceria público-privada realizada para a construção do Parque Olímpico, através da análise dos documentos relacionados ao seu processo licitatório e a escolha do consórcio vencedor. Ainda, como esse empreendimento tem impactado no caso da comunidade Vila Autódromo, que além de já ter sido alvo de outras tentativas de remoção, foi objeto de uma política de regularização fundiária no passado e tem um intenso histórico de resistência e de luta pela permanência. / This research aims to investigate an ongoing process, where the strategy of entrepreneurship in the management of urban public policy has been adapting and conforming to the current context of economic policy called the neo-developmentism. Thus, the role of public-private partnerships stands as fundamental in the management of urban development within the three spheres of power. Discuss, therefore, the history of political reforms in the country that led to the adaptation of national developmentalism to the new developmentalism policy, to understand the context in which thec urrent public-private partnerships have been developed as a model for managing the territory. Also mention the legislation and discourses behind the defense of this kind of deal between the government and the private sector. Still, as the country faced a crisis in 2008, called the crisis of crises, using this model of land management. The megasporting events will be analyzed as strong attractives of investmentand business to the cities. In Rio de Janeiro, with the choice of city to host the Olympic Games, and also being one of the host cities of the FIFA World Cup in 2014, several of territorial transformations began. In addition to evidence that a series of violations were to come in order to make room and expand the market for private investments. The Olympic Park, the main site of the 2016 Olympics competitions, appears as an immense business opportunity with private enterprise, locatedon a broad expansion of the real estate market in the city: Barra da Tijuca. Thus many poor communities have suffered from the policy of forced displacement to make way for investments in theterritory. In this context, is the Vila Autódromo community, well established local side of the venture. The removal of this community is planned in the feasibility study and in de concession contract. Therefore, we will study the public-private partnership held for the construction of the Olympic Park, through the analysis of documents related to the bidding process and the selection of the winning consortium. Yet, as this project has impacted the case of Vila Autódromo community, which in addition to having been the target of other attempts of eviction, was subject to a policy of land regularization in the past and has a strong history of resistance and struggle for permanence.
196

Empreendedorismo urbano e práticas de planejamento : a copa do mundo e os grandes projetos urbanos em Porto Alegre

Oliveira, Clarice Misoczky de January 2013 (has links)
O objetivo deste trabalho é a análise das práticas de planejamento urbano vigentes em Porto Alegre em sua relação com os grandes projetos urbanos (GPUs) associados à realização da Copa do Mundo 2014. Para tanto, foram abordados os conceitos de empresariamento, gerencialismo e marketing urbano com a finalidade de compreender o conceito de empreendedorismo urbano. Buscou-se compreender, também, as relações entre empreendedorismo urbano, práticas de planejamento, GPUs e megaeventos. A pesquisa, realizada com base em estudo de caso único, integrado por três unidades de análise (mobilidade, estádios e orla), envolveu o estudo de 18 projetos. As análises feitas exploraram as múltiplas dimensões de GPUs, buscando conhecer o contexto em que os projetos surgiram e como se justificaram, bem como identificar as práticas de planejamento, os atores envolvidos, possíveis desdobramentos socioambientais, bem como as fontes de recursos a eles associados. Por fim, analisou-se a relação entre o processo e estratégias típicas do empreendedorismo urbano, indicando seus vínculos com os grandes projetos. / This work aims to analyses the urban planning practices present in Porto Alegre, and its relation with large urban projects being articulated for the FIFA World Cup 2014 mega event. The concepts addressed are entrepreneurialism, managerialism and urban marketing in its original fields to comprehend how Harvey built the urban entrepreneurialism concept and its best translation to Portuguese. This work aims, as well, comprehend the relation between urban entrepreneurialism and urban planning practices, large urban projects and mega events. This is a case study research with 3 unities of analysis incorporated: 1) mobility; 2) stadia; 3) water front. Those unities, therefore, are constituted by 18 different projects related to the FIFA World Cup, in a direct or indirect fashion. Their analyses are built by exploring its multiple dimensions regarded to large urban projects, by understanding the context where those projects have been created and how they are justified, by describing the projects elaboration processes to identify the urban planning practices related and its social and environmental impacts associated, and by analyzing the relation between this process and the urban entrepreneurialism strategies assigned by Harvey, indicating which project belongs to each strategies.
197

Moçambique : uma longa caminhada para um futuro incerto?

Matsinhe, Leví Salomão January 2011 (has links)
A trajetória de Moçambique desde a independência, ocorrida em 1975, até 2009 foi marcada pela transição de uma economia centralmente planificada (socialista) para uma economia de mercado (capitalista). A transição para o capitalismo abriu espaço para financiamentos de Ajuda Externa ao Desenvolvimento das Instituições de Bretton Woods (Banco Mundial – BM e Fundo Monetário Internacional – FMI) e Investimentos Estrangeiros Diretos em forma de mega-projetos das corporações multinacionais em Zonas Francas Industriais e Zonas Econômicas Especiais. Entretanto, a aliança da elite nacional como o capital estrangeiro favoreceu a corrupção, a transferência de lucros das multinacionais para os países de origem, fazendo com que a Ajuda Externa ao Desenvolvimento e os Investimentos Estrangeiros Diretos não produzissem desenvolvimento econômico e bem-estar social, com se pretendia que fosse. Este estudo identificou o Ajustamento Estrutural, a Ajuda Externa ao Desenvolvimento, a corrupção e as Zonas Francas Industriais como os principais vilões da situação de pobreza em que Moçambique se encontra e concluiu que Moçambique nunca irá reduzir a pobreza de forma significativa, contando apenas com ajuda externa, mega-projetos, investimentos estrangeiros e ZFI. O que se pensava que seria o remédio para a saída do marasmo do subdesenvolvimento de Moçambique, (portanto, a ajuda, mega-projetos e ZFI) tornou-se um obstáculo ao desenvolvimento do país. / The trantion from socialism to capitalism characterized the Mozambican trajectory after its independence, held in 1975. This transition gave place to external aid by Bretton Woods (World Bank and International Monetary Fund) and direct investments from multinational corporations mega-projects in special economic free zones. The alliance between the national elite with foreign capital brought corruption, transference of profits from the multinational corporations in Mozambique to its countries of origin. Because of this, the external aid and foreign investments did not develop the Mozambican economy and also did not bring the social well being to the people in Mozambique. This research, identified the structural adjustment, external aid, corruption and special economic free zones as the factories of poverty in Mozambique, and concluded that Mozambique will never reduce poverty looking to external aid, mega-projects, direct foreign investments and special economic free zones. What where thought it was an elixir (mega-projects, external aid, foreign direct investments and special economic free zones) became an obstacle to development.
198

Empreendedorismo urbano e práticas de planejamento : a copa do mundo e os grandes projetos urbanos em Porto Alegre

Oliveira, Clarice Misoczky de January 2013 (has links)
O objetivo deste trabalho é a análise das práticas de planejamento urbano vigentes em Porto Alegre em sua relação com os grandes projetos urbanos (GPUs) associados à realização da Copa do Mundo 2014. Para tanto, foram abordados os conceitos de empresariamento, gerencialismo e marketing urbano com a finalidade de compreender o conceito de empreendedorismo urbano. Buscou-se compreender, também, as relações entre empreendedorismo urbano, práticas de planejamento, GPUs e megaeventos. A pesquisa, realizada com base em estudo de caso único, integrado por três unidades de análise (mobilidade, estádios e orla), envolveu o estudo de 18 projetos. As análises feitas exploraram as múltiplas dimensões de GPUs, buscando conhecer o contexto em que os projetos surgiram e como se justificaram, bem como identificar as práticas de planejamento, os atores envolvidos, possíveis desdobramentos socioambientais, bem como as fontes de recursos a eles associados. Por fim, analisou-se a relação entre o processo e estratégias típicas do empreendedorismo urbano, indicando seus vínculos com os grandes projetos. / This work aims to analyses the urban planning practices present in Porto Alegre, and its relation with large urban projects being articulated for the FIFA World Cup 2014 mega event. The concepts addressed are entrepreneurialism, managerialism and urban marketing in its original fields to comprehend how Harvey built the urban entrepreneurialism concept and its best translation to Portuguese. This work aims, as well, comprehend the relation between urban entrepreneurialism and urban planning practices, large urban projects and mega events. This is a case study research with 3 unities of analysis incorporated: 1) mobility; 2) stadia; 3) water front. Those unities, therefore, are constituted by 18 different projects related to the FIFA World Cup, in a direct or indirect fashion. Their analyses are built by exploring its multiple dimensions regarded to large urban projects, by understanding the context where those projects have been created and how they are justified, by describing the projects elaboration processes to identify the urban planning practices related and its social and environmental impacts associated, and by analyzing the relation between this process and the urban entrepreneurialism strategies assigned by Harvey, indicating which project belongs to each strategies.
199

Porto Maravilha: arbitrariedade, mídia e produção de consenso na construção da nova região portuária do Rio de Janeiro

Silva, Ana Cristina Costa de Lima e 12 June 2017 (has links)
Submitted by Programa de Pós-Graduação em Mídia e Cotidiano (ppgmc@vm.uff.br) on 2017-05-02T21:10:29Z No. of bitstreams: 1 DISSERTACAO COMPLETA - ENTREGA FINAL - ANA COSTA.pdf: 1543810 bytes, checksum: 18f5ddbdda9120f220695f92c2bd844b (MD5) / Approved for entry into archive by Josimara Dias Brumatti (bcgdigital@ndc.uff.br) on 2017-06-12T15:52:20Z (GMT) No. of bitstreams: 1 DISSERTACAO COMPLETA - ENTREGA FINAL - ANA COSTA.pdf: 1543810 bytes, checksum: 18f5ddbdda9120f220695f92c2bd844b (MD5) / Made available in DSpace on 2017-06-12T15:52:20Z (GMT). No. of bitstreams: 1 DISSERTACAO COMPLETA - ENTREGA FINAL - ANA COSTA.pdf: 1543810 bytes, checksum: 18f5ddbdda9120f220695f92c2bd844b (MD5) / FAPERJ / A escolha do Rio de Janeiro como sede das Olimpíadas de 2016 consagrou a implantação de um modelo de desenvolvimento urbano baseado na lógica da cidade-empresa. Orientada pela organização da administração da cidade a partir de características empresariais, a gestão municipal fomentou a elaboração de projetos de infraestrutura capazes de atender às exigências do Comitê Olímpico Internacional (COI) para a realização dos Jogos. Entre eles está o Porto Maravilha, projeto de concessão à iniciativa privada da região portuária da cidade, detentora de grande carga histórica e cultural na formação do Rio. Sendo assim, esse trabalho propõe compreender se o processo de desenvolvimento e execução do Porto Maravilha corresponde ao discurso de legado prometido pelo poder público a todos os moradores da região do Porto. Partindo da análise de seis anos do noticiário do jornal O Globo, a pesquisa busca entender que imagem de cidade é possível apreender a partir das representações trazidas pelo periódico. / The choice of Rio de Janeiro to host the 2016 Olympics established the implementation of an urban development model based on business-city logic. Oriented by the organization of city administration from entrepreneurial characteristics, municipal management has fostered the development of infrastructure projects able to meet the requirements of the International Olympic Committee (IOC) for the Games. Among them is Porto Maravilha, concession project to the private sector of the port area of the city, which holds great historical and cultural background in the formation of Rio. Thus, this work aims to understand if the development and execution process of Porto Maravilha matches the legacy speech promised by the government to all residents of the port area. Based on six years of news analysis from O Globo newspaper, this research seeks to understand what city image is possible to learn from the representations brought by the journal.
200

Molecular markers of gliomas : implications for diagnosis and new target therapies / Les marqueurs moléculaires de gliomes : implications pour diagnostics et nouvelles thérapies cibles

Di Stefano, Anna Luisa 21 February 2017 (has links)
Le travail de thèse est dédié à la caractérisation de fusions spécifiques oncogéniques entre les gènes FGFR et TACC dans les gliomes. Nous avons analysé 907 gliomes pour la présence du gène de fusion FGFR3-TACC3. Nous avons montré que les fusions FGFR3-TACC3 ne touchent que les gliomes IDH wild-type (3%), sont mutuellement exclusives avec l'amplification de EGFR et avec la forme tronquée EGFRvIII et inversement, sont associées à l'amplification de CDK4 et de MDM2 et à la délétion du 10q. Les fusions FGFR3-TACC3 sont associées à une expression intense et diffuse de FGFR3 en immunohistochimie (IHC) et l'IHC pour FGFR3 est un marqueur prédictif très sensible de la présence des fusions FGFR3-TACC3. Les patients porteurs d'une fusion FGFR3-TACC3 ont une survie globale significativement plus longue comparés aux patients avec gliome IDH wild-type. Nous avons traité deux patients porteurs d'un gène de fusion FGFR3-TACC3 avec un inhibiteur tyrosine-kinase (TK) spécifique pour FGFR et nous avons observé une stabilisation de maladie et une réponse mineur chez un patient. Dans la deuxième section nous avons optimisé une nouvelle séquence de spectroscopie différentielle-MEGA-PRESS-pour la détection de l'oncometabolite 2-hydroxyglutarate (2 HG) qui s'accumule de manière spécifique dans les gliomes IDH mutés. Nous avons analysé de façon prospective une cohorte de 25 patients avant chirurgie pour probable gliome de grade II et grade III. Nous avons trouvé que la MEGA-PRESS est hautement spécifique (100%) et sensible (80%) dans la prédiction de la présence de la mutation IDH. Son taux est corrélé aux concentrations de 2 HG mesurés sur tissu congelé par spectrométrie de masse (GC-MS/MS). / This work is devoted to the characterization of a specific oncogenic fusion between FGFR and TACC genes in gliomas. Overall, we screened 907 gliomas for FGFR3-TACC3 fusions. We found that FGFR3-TACC3 fusions exclusively affect IDH wild-type gliomas (3%), and are mutually exclusive with the EGFR amplification and the EGFR vIII variant, whereas it co-occurs with CDK4 amplification, MDM2 amplification and 10q loss. FGFR3–TACC3 fusions were associated with strong and homogeneous FGFR3 immunostaining. We show that FGFR3 immunostaining is a sensitive predictor of the presence of FGFR3-TACC3 fusions. FGFR3-TACC3 glioma patients had a longer overall survival than those patients with IDH wild-type glioma. We treated two patients with FGFR3–TACC3 rearrangements with a specific FGFR-TK inhibitor and we observed a clinical improvement in both and a minor response in one patient. In the second section, we developed a non-invasive diagnostic tool by 1H-magnetic resonance spectroscopy in IDH mutant gliomas. We optimized a uniquely different spectroscopy sequence called MEGA-PRESS for the detection of the oncometabolite 2-hydroxyglutarate (2 HG) that specifically accumulates in IDH mutant gliomas. We analysed a prospective cohort of 25 patients before surgery for suspected grade II and grade III gliomas and we assessed specificity and sensitivity, correlation with 2 HG concentrations in the tumor and associations with grade and genomic background. We found that MEGA-PRESS is highly specific (100%) and sensitive (80%) for the prediction of IDH mutation and correlated with 2 HG levels measured by gas chromatography-tandem mass spectrometry (GC-MS/MS) in frozen tissue.

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