Abstract Pasture is a very species-rich but threatened nature type, and compared to 200 years ago, only five percent of natural pastures remain in Sweden. One way to try to save the biological diversity that pastures harbor is to restore overgrown land by clearing and reintroducing grazing animals. At the site chosen for this study, Hallands Väderö, 92 ha of pasture have been restored during less than ten years. The purpose of this thesis is to compare species richness in vegetation on recently restored land (cleared 2-10 years ago), land that has been grazed continuously for a long time and overgrown pasture, and to investigate whether the number of plant species increases with time after a pasture restoration. Thirteen locations on the island were selected, and three randomly selected square meter squares per area were inventoried. Each area was then searched for more species that were not present in the random squares. The data underwent analysis using regression analysis, ANOVA and Tukey test. Results indicated no significant relationship between the number of species in an area and the time (years) after a restoration. The Tukey test indicated that the biological diversity (effective number of species) was statistically significant higher on continuously grazed areas than on overgrown areas and that the number of species was statistically significant higher on restored areas than on overgrown areas. This means that there is an effect of the management; the number of species increases after grazing restoration, and biodiversity is higher on continuously grazed land than on overgrown land. The fact that the overgrown pasture has the lowest number of species shows that we risk losing species if pastures are abandoned and overgrown. / Sammanfattning Betesmark är en väldigt artrik men hotad naturtyp och jämfört med för 200 år sedan finns endast fem procent av naturbetesmarkerna i Sverige kvar. Ett sätt att försöka bibehålla den biologiska mångfalden, som betesmark hyser, är att restaurera igenväxt mark genom röjning och återintroduktion av betesdjur. På den valda platsen för denna studie, Hallands Väderö, har 92 ha mark betesrestaurerats under knappt tio års tid. Syftet med detta examensarbete är att jämföra artrikedom i växtligheten på nyligen restaurerad mark (röjd för 2–10 år sedan), mark som betats kontinuerligt under lång tid och igenväxt betesmark samt att undersöka om antalet växtarter ökar med tiden efter en betesrestaurering. Tretton platser på ön valdes ut och tre slumpvis valda kvadratmeterstora rutor per område inventerades. Varje område genomsöktes sedan efter fler arter som inte fanns i de slumpade rutorna. Datan analyserades med regressionsanalys, ANOVA och Tukey-test. Resultatet visade att det inte fanns något signifikant samband mellan antal arter på ett område och tiden (år) efter en restaurering. Tukey-testet visade att den biologiska mångfalden (effektivt artantal) var statistiskt signifikant högre på kontinuerligt betade områden än igenväxta områden och att antal arter är statistiskt signifikant fler på restaurerade områden än igenväxta områden. Detta betyder att det finns en effekt av skötseln; antal arter ökar efter en betesrestaurering och den biologiska mångfalden är högre på kontinuerligt betad mark än igenväxt mark. Att den igenväxta betesmarken har lägst antal arter visar på att vi riskerar att förlora arter om betesmarker överges och växer igen.

<p>Land use change constitutes a primary driving force in shaping social-ecological systems world wide, and its effects reach far beyond the directly impacted areas. Graph based landscape ecological tools have become established as a promising way to efficiently explore and analyze the complex, spatial systems dynamics of ecological networks in physical landscapes. However, little attention has been paid to making these approaches operational within ecological assessments, physical planning, and design. This thesis presents a network based, landscape-ecological tool that can be implemented for effective use by practitioners within physical planning and design, and ecological assessments related to these activities. The tool is based on an ecological profile system, a common generalized network model of the ecological infrastructure, graph theoretic metrics, and a spatially explicit, geographically defined representation, deployable in a GIS. Graph theoretic metrics and analysis techniques are able to capture the spatio-temporal dynamics of complex systems, and the generalized network model places the graph theoretic toolbox in a geographically defined landscape. This provides completely new insights for physical planning, and environmental assessment activities. The design of the model is based on the experience gained through seven real-world cases, commissioned by different governmental organizations within Stockholm County. A participatory approach was used in these case studies, involving stakeholders of different backgrounds, in which the tool proved to be flexible and effective in the communication and negotiation of indicators, targets, and impacts. In addition to successful impact predictions for alternative planning scenarios, the tool was able to highlight critical ecological structures within the landscape, both from a system-centric, and a site-centric perspective. In already being deployed and used in planning, assessments, inventories, and monitoring by several of the involved organizations, the tool has proved to effectively meet some of the challenges of application in a multidisciplinary landscape.</p>

Land use change constitutes a primary driving force in shaping social-ecological systems world wide, and its effects reach far beyond the directly impacted areas. Graph based landscape ecological tools have become established as a promising way to efficiently explore and analyze the complex, spatial systems dynamics of ecological networks in physical landscapes. However, little attention has been paid to making these approaches operational within ecological assessments, physical planning, and design. This thesis presents a network based, landscape-ecological tool that can be implemented for effective use by practitioners within physical planning and design, and ecological assessments related to these activities. The tool is based on an ecological profile system, a common generalized network model of the ecological infrastructure, graph theoretic metrics, and a spatially explicit, geographically defined representation, deployable in a GIS. Graph theoretic metrics and analysis techniques are able to capture the spatio-temporal dynamics of complex systems, and the generalized network model places the graph theoretic toolbox in a geographically defined landscape. This provides completely new insights for physical planning, and environmental assessment activities. The design of the model is based on the experience gained through seven real-world cases, commissioned by different governmental organizations within Stockholm County. A participatory approach was used in these case studies, involving stakeholders of different backgrounds, in which the tool proved to be flexible and effective in the communication and negotiation of indicators, targets, and impacts. In addition to successful impact predictions for alternative planning scenarios, the tool was able to highlight critical ecological structures within the landscape, both from a system-centric, and a site-centric perspective. In already being deployed and used in planning, assessments, inventories, and monitoring by several of the involved organizations, the tool has proved to effectively meet some of the challenges of application in a multidisciplinary landscape.

Vid blodgivning donerar blodgivare blod frivilligt. Blodet kan sedan användas inom sjukvården för exempelvis blodtransfusion, vilket kräver blodprodukter kompatibla med patienten. Förekomst av leukocyter i blodprodukter medför en ökad risk för febrila transfusionsreaktioner hos transfunderade patienter. Därför krävs det att vid framställning leukocytreducera blodprodukter och utföra kvalitetskontroll. Med analysen B-leukocytpartikelkoncentration (LPK) kan totalantalet leukocyter i helblod beräknas. Flödescytometri är en metod som kan analysera optiska och fluorescerande egenskaper hos exempelvis celler i en suspension, vilket kan användas för att kvantifiera cellantal. BD Leucocount™-Kit (BD Biosciences) är avsett för flödescytometrisk analys av antalet kvarvarande leukocyter i leukocytreducerade blodprodukter. Vid framställning av blodprodukter ska helblodspåsen vila vid rumstemperatur i minst 3 timmar efter blodgivning. I Falun används antingen ett dagsprogram där produktion sker samma dag som blodgivningen, eller ett övernattningsprogram där produktion sker dagen därpå. Prover från 505 kontrollerade erytrocytenheter, samlade i Falun, har påvisat en skillnad i leukocytkoncentration beroende på vilket program som använts. Anledningen till att erytrocytenheternas leukocytinnehåll skiljer sig är inte känt. Syftet med denna studie är därav att undersöka om vilotiden har någon effekt på leukocytkoncentrationen i helblodspåsar. LPK varierade mellan helblodspåsarna. Ett ökande leukocytantal observerades över tid i majoriteten av helblodspåsar, inklusive medelvärde. Däremot kunde inte hypotesprövning påvisa statistisk signifikans. Hypotesen om att leukocytantalet ökar över tid går emot grundläggande hematologi. Utifrån resultaten i denna studie kan inte hypotesen bevisas. Vidare studier bör genomföras. / During blood donation, blood donors donate blood voluntarily. The blood can then be used in healthcare for, for example, blood transfusions, which requires blood products compatible with the patient. The presence of leukocytes in blood products increases the risk of febrile transfusion reactions in transfused patients. Therefore, leukocyte-reduction in blood products is necessary during production. Each blood center must perform quality control on produced blood products. With the analysis B-leukocyte particle concentration (LPK), the total number of leukocytes in whole blood can be calculated. Flow cytometry is a method that can analyze the optical and fluorescent properties of, for example, cells in a suspension, which can be used to quantify cell numbers. The BD Leucocount™-Kit (BD Biosciences) is intended for flow cytometric analysis of the number of leukocytes remaining in leukocyte-reduced blood products. When producing blood products, the whole blood bag should rest at room temperature for at least three hours after the donation. In Falun, either a day program is used where production takes place on the same day as the blood was donated, or an overnight program where production takes place the next day. Samples from 505 controlled erythrocyte units, collected in Falun, have shown a difference in leukocyte concentration depending on the program used. The reason why the leukocyte content of erythrocyte units differs is not known. The purpose of this study is therefore to investigate whether the resting period has any effect on the leukocyte concentration in whole blood bags. The LPK varied between the whole blood bags. An increasing leukocyte count was observed over time in most of the whole blood bags. However, hypothesis testing did not show statistical significance. The hypothesis that leukocyte counts increase goes against basic hematology. Based on the results of this study, the hypothesis cannot be proven. Further studies should be conducted. / <p>Vårdförbundet tilldelade Leo Svahn stipendium 2021 för <em>bästa kandidatuppsats inom biomedicinsk laboratorievetenskap</em>.</p>