With the advent of Tyrosine kinase inhibitors (TKI) as a therapy for Chronic myeloid Leukemia (CML), the patients now enjoy a life expectancy close to that of the general population. But some patients do get unresponsive to the TKI treatment over time due to several mutations in the kinase domain of the BCR-ABL fusion protein, which further leads to activation of multiple signaling cascades within the leukemic cell, helping it survive and proliferate. This project validates and optimizes a new method of In situ PLA that incorporates the usage of different padlocks and template oligos. Multiple cross-reactivity tests and interaction assays in multiple cancer cell lines will further optimize this system as a robust multiplex protein-protein interaction detection tool. Proteins associated with the MAP-K, PI3-K, and Jak-STAT signaling pathways were the main detection targets.

Syftet med studien är att undersöka hur lärare i den svenska grundskolan arbetar för att få till en lyckad undervisning i ämnet sexualitet och relationer trots hinder i form av religiösa och kulturella värderingar samt föräldrars påverkan på eleverna. Studien grundar sig i en kvalitativ forskning och empirin har samlats in genom kvalitativa intervjuer med sju lärare. Intervjuerna spelades in och transkriberades i efterhand för att slutligen kunna analyseras. Intervjuerna var semistrukturerade och ledde till en bred förståelse för hur lärare känner, tänker och arbetar för en bra undervisning i sexualitet och relationer. Resultatet visade att lärare upplever att det finns hinder i undervisningen i form av kulturella och religiösa värderingar men även föräldrars påverkan på eleverna som utmanar lärarna men med hjälp av att ta stöttning från bland annat elevhälsan och ledningen lyckas lärarna få till en god undervisning. Lärarnas tillvägagångsätt för en lyckad undervisning grundar sig i att sätta eleverna i fokus, arbeta för att skapa tillit mellan lärare och elever, skapa en trygg klassrumsmiljö, börja introducera ämnet i tidig årskurs och att ha anpassade undervisningsgrupper.

The global amphibian population is rapidly declining. Although many threats that affect amphibians are known, there are many contributing factors which are not fully understood. For adult amphibians, the largest part of the mortality comes from predation. We know of many predators which prey upon amphibians, but to what extent they affect the populations has not been subject to much investigation. Neither has the effects of specific taxa or species of predators. Birds are reputed amphibian-eaters, and they often occur in the same habitats. Both birds and amphibians often select for wetlands and other water bodies with high biodiversity, that often support a high diversity and/or density of birds as well as amphibians. The fact that a prey might flourish in an area that one of its strongest predators also inhabits might seem controversial. Especially when taking into account that it is often the density of predators that best explains the population growth of prey species. In this review, I investigate what effects large amounts of birds might have on amphibian populations as a result of their predation on adults. I consider how the effects might differ with high vs low densities of both prey and predator, and whether an eventual effect might impact the survival of a metapopulation differently depending on its size and spatial distribution. As an example of a habitat where both bird and amphibian species occur in large numbers, I use Trönninge ängar - a bird conservation area just outside the city of Halmstad, where populations of both amphibians and birds have been increasing over the last years. My results show that high densities of predatory birds in such communities could potentially cause declines in amphibian populations, but that this impact is softened when the population is more widely distributed in the area. There are also possibilities that birds do not only affect amphibians by predation, but also might facilitate their distribution by reducing other predators. Thus, bird predation in itself might not be a sufficient predictor for their effect on amphibian populations, as the relationship might be more complex than simple predator-prey interactions.

The aim of this study is to understand from a professional perspective the importance of the biological children’s participation in the family home process and how family home social workers use their room for maneuver to involve the biological children in the family home process. The method used to answer the aim and the research questions was semi-structured interviews with seven women that work as family home social workers and the empirical material was analyzed through a thematic analysis. The theoretical framework we used to analyze the results was Lipsky's theory of Street-level bureaucracy and discretion. The main findings of this study were that family home social workers think that the participation of biological children is important both for the child's own wellbeing, for the whole family's dynamics and for the outcome of a family home placement. The most common methods used by family home social workers to involve biological children are relationship building and different types of conversations depending on the children's age and maturity. The study showsin the family home investigation, there are formalized guidelines that the family home social workers work according to but there are no general guidelines in the work with biological children in the further family home placement. That results in a discretion for the family home social workers. The study shows opportunities for improvement in making the biological children more involved in the family home process and that the biological children's participation is important for the work of family home social workers. Our results both confirm and contrast previous international and national research presented in the study.

The forest area in which this biotope survey was executed is located east of Mjälahult, in the county of Hylte. East of the area lies the lake Stora Allgunnen, which is part of the main catchment area of Nissan and contains perch, bream, pike, whitefish and pike-perch. The examined forest is a deciduous forest consisting of beech and oak, with elements of birch, rowan, hazel, spruce, and pine. There are signs that the forest was formerly used as a pasture, with old stone walls surrounding the entire area and an old road crossing the middle of the forest. The area consists of a lot of moss-covered stones, stone cairns, dead wood, beard lichens and signs from great spotted woodpecker. The main purpose of this biotope survey is to evaluate biotopes that are of value for biodiversity, in order to protect valuable nature. This biotope survey has been executed according to the national Swedish standard for biodiversity surveys (SS 199000:2014), with an addition for value elements. During the inventory, four different biotopes were identified - mixed forest, beach forest, mixed swamp forest and noble deciduous forest. Two biotopes were identified as biotopes of considerable biotope value - mixed swamp forest and noble deciduous forest. The third biotope - beach forest was identified as a biotope of certain biotope value. Parts of the area - mixed forest, was identified as a biotope of no considerable biotope value. Even though only two of four biotopes have a considerable biotope value, the objects that have a significant value cover large areas of the forest, which raises interest in preserving and protecting the entire forest in the long term, considering that it will promote biodiversity in the future. / Ögärdet skog ligger öster om Mjälahult i Hylte kommun. Öster om skogen ligger sjön St. Allgunnen, vilken ingår i Nissans huvudavrinningsområde och där man bland annat kan hitta abborre, braxen, gädda, sik och gös. Den inventerade skogen är en ädellövskog som består av bok och ek, med inslag av björk, rönn, hassel, gran och tall. Det finns tecken på att området förr har använts som betesskog med gamla stenmurar som inhägnar området. Det finns även tydliga tecken på en gammal väg som korsar den nuvarande grusvägen och in i skogen. Området består av mycket mossbeklädda stenar, stenrösen, död ved, skägglavar och spår från större hackspett. Det huvudsakliga syftet med denna biotopinventering är att värdera biotoper som är utav värde för biologisk mångfald, samt för att utveckla ett skydd för dessa biotoper. Arbetet är utfört enligt SIS standard (SS 199000:2014), med tillägg värdeelement. Under inventeringen identifierades fyra olika biotoper – blandskog, strandskog, blandsumpskog och ädellövskog. Två biotoper identifierades med ett påtagligt biotopvärde, vilka var ädellövskog och blandsumpskog. Den tredje biotopen som identifierades var strandskog, vilken bedömdes ha ett visst biotopvärde. Delar av området identifierades även till biotopen blandskog, vilka bedömdes ha ett obetydligt biotopvärde. De värdeelement som påträffades täcker stora ytor av skogen och har ett högt naturvärde, vilket ger upphov till ett intresse att bevara och skydda skogen långsiktigt med förutsättning att den i framtiden kan gynna biologisk mångfald.

Abstract The management and handling of organic waste in primarily low-income developing cities, due to high urbanization has become a global problem. The main form of waste handling in these cities are landfilling, due to it being the cheapest and easiest option. Not only are nutrients wasted, but landfills of mixed waste create strong emission of greenhouse gasses and a leachate that affects groundwater quality. This major problem in addition to a higher demand for food due to the increase of the world population leaves us two problems to solve; to handle waste and produce food. The Black Soldier Fly (Hermetia illucens) and its larvae can solve both of these problems in one. The organic waste is fed to the fly larvae and the larvae produce biomass (larval weight) and a residue similar to un-mature compost. The larvae can then be fed live to animals or made into pellets to feed animals such as fish, and the residue can be used as fertilizer. However, in order for the larvae to be able to be extruded into pellets or fed live to ex. hens, an optimal protein and fat content of the larvae is needed. A higher protein and lower fat content of the larvae is optimal for both pellet production and to feed to animals. It has previously been found that an inclusion of mussels can lower the fat content and increase the protein content of the larvae. In this article, different amounts of mussels are included in the larvae diet apart from bread and cabbage to see if it has an effect on the compost process efficiency. The experiment is done by larvae being added to different ratios of bread: cabbage: mussels. The larvae are then harvested and process efficiency parameters; BCE (biomass conversion efficiency), survival percentage, material reduction and respiration are calculated. It was found that mussel inclusion was equivalent to a higher feeding load per larva. The BCE of the treatment with a higher feeding load and the treatment with the highest mussel inclusion were the same, meaning that mussel inclusion is equivalent to a diet with high amounts of carbohydrates. Mussel inclusion in the larva diet seems to be beneficial from a BCE standpoint and suits as a substrate as pH and survival was not negatively affected by the mussels.

Malaria is a serious disease that can lead to fatal consequences if not treated. It is mainly spread via Plasmodium falciparum, a parasite carried by mosquitoes as host organisms. As a potential way of treating malaria, research is being done on possible inhibitors of calmodulin (CaM) in the parasite. CaM is a highly conserved protein found in all eukaryotes, and is important in many essential biochemical reactions. The potential inhibitor analysed in this study is calmidazolium (CZM). This study aims to characterise structure and stability differences between the C-lobes of human and P. falciparum CaM, while analysing the effect of the presence of CZM.  Previous studies have proven that CZM acts as an inhibitor to human CaM by binding to the C-lobe, with a dissociation constant in the nano molar range. In other studies, thermal stability measurements have shown that the secondary structure of P. falciparum CaM is more stable than that of human CaM.  In this study, the stability measurements showed that for the ANS binding site and around tyrosines, the C-lobe of human CaM was more stable than the C-lobe of P. falciparum CaM, knowledge which was previously unknown. When studying the entire secondary structure, the C-lobe of P. falciparum CaM was found to be more stable, which is in agreement with previous studies for the secondary structure of the complete CaM variants. For binding, the dissociation constants for both the C-lobe of human CaM and for the C-lobe of P. falciparum CaM were proven to be at a lower range than micro molar, most likely in the nano molar range. This is in agreement with earlier findings regarding the entire human CaM. Furthermore, CaM and CZM were proven to have their absorbance at the same wavelengths. Finally, several amino acid differences between the C-lobes of human and P. falciparum CaM were found that could play a role in binding and stability. One specific amino acid that was suggested to contribute to the stabilisation of the C-lobe of P. falciparum CaM was isoleucine. In the C-lobe of human CaM, these isoleucines were exchanged to threonine and arginine. Another amino acid difference that could potentially play a key role was the valine versus isoleucine, where valine might contribute to the stabilisation of the ANS binding site of the C-lobe of human CaM. To perform this study, the methods fluorescence spectroscopy, UV spectroscopy and circular dichroism were used, as well as several bioinformatic tools.  Overall, both stability and structure analyses have helped determine several differences between the two CaM variants, opening up possibilities to find an inhibitor that targets only the CaM of P. falciparum. CZM still remains as an interesting potential inhibitor, and can hopefully be a part of future research in malaria treatment.

Loose smut of barley, caused by fungal pathogen Ustilago nuda is one of the major concerns throughout the globe for barley producers. The infection takes place without exhibiting any obvious symptoms and an infected seed lot can only be identified at the heading stage when the fungal teliospores emerge at the place of crop. The percentage losses on yield are directly proportional to the occurrence of infection. Currently available detection methods include seed health testing protocols which are time-consuming and cumbersome. With the globalization of the international market and increased crop demand, development of rapid disease screening methodologies has become an essential focus in the field of plant pathology. The present study sought to develop a rapid probe-based detection method for screening of U. nuda with real-time qPCR. Two U. nuda specific primer pairs were compared using standard PCR alongside optimization of real-time qPCR assay. The advantage of high fidelity DNA polymerase for amplification of U. nuda genomic DNA was recorded. U. nuda genomic DNA was amplified and cloned into a vector which was further used for generation of a quantification curve with a specific probe. The qPCR assay developed in this study was successful in the detection of as little as 43 copies of U. nuda genomic DNA. With studies involving larger sample size and field samples, this assay can be improved for enhanced sensitivity and specificity which can help in monitoring infection from DNA extractions of barley seeds and further improving the current microscopic detection methods.

Actinorhizal plants are in symbiosis with the nitrogen-fixating soil bacterium Frankia, which forms nodules in the plant root. However, several studies also report other endophytic bacteria appearing in the nodules, but their function and interaction with the host plant or Frankia is not yet understood. This thesis used a bioinformatic approach to investigate the metagenomes of eighteen actinorhizal nodule samples to find out which bacteria are present, how the microbiomes differed from each other, and if the genomes of non-Frankia inhabitants could give indications of any functions. The results showed that the bacterial composition, richness, and diversity differed among the samples, especially between the samples sequenced from the field versus those primarily cultivated in a greenhouse. All samples had a substantial number of sequencing reads belonging to potential endophytes, such as strains of Enterobacteria, Pseudomonas, Streptomyces, Micromonospora, Mycobacteria and Pseudonocardia. There seemed to be a common microbial community shared among the plants on a family level, since no significant difference was found in the core microbiomes between the field and greenhouse groups. Some sequences found in the metagenomes were annotated as potential functions of the fellow travellers, such as antibiotic synthesis, proteins involved in regulating abiotic stresses, but also probable plant damaging compounds rather associated with pathogens than symbionts.

In cities today, vast amounts of nutrients are being wasted. Improvement in nutrient management within agriculture can contribute to a more sustainable society. Reusing nutrients in agriculture could aid in creating a more circular system, where organic fertilizers can be used instead of chemical fertilizers. Urine is a liquid which has a high nutrient content. According to the Swedish environmental protection agency, human urine can replace mineral fertilizers, by using methods such as source separation, where urine is divided from faeces. This is a cheap, effective and sustainable fertilizer management system that can be easily achieved. In this study, urine fertilizers were compared with ecological and conventional fertilizers (NPK and cow manure). The study examined the effect of different urine fertilizers compared with organic and inorganic ones on plant growth, nutrient content, pH value and microbial growth. The plant growth experiment was carried out in the greenhouse facilities in Alnarp, Sweden. The results from the experiment show that cow manure has a better outcome when it comes to plant growth, but Aurin, one of the urine fertilizers, had the highest uptake of nitrate. Non-diluted urine had a stable result in all analyses. According to this study human urine is a fertilizer which can be used in crop cultivation systems, and can deliver good agricultural results.