Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-03-12T14:16:55Z No. of bitstreams: 1 WaleskaCristinaEuflausino_DISSERT.pdf: 2155309 bytes, checksum: 4d1605e644787439d12a4dd235990b92 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-03-14T19:58:05Z (GMT) No. of bitstreams: 1 WaleskaCristinaEuflausino_DISSERT.pdf: 2155309 bytes, checksum: 4d1605e644787439d12a4dd235990b92 (MD5) / Made available in DSpace on 2018-03-14T19:58:05Z (GMT). No. of bitstreams: 1 WaleskaCristinaEuflausino_DISSERT.pdf: 2155309 bytes, checksum: 4d1605e644787439d12a4dd235990b92 (MD5) Previous issue date: 2016-12-16 / T?cnicas de controle estat?stico de processos (CEP), comumente usadas em problemas industriais, podem tamb?m ser aplicadas na ?rea da sa?de, tendo em vista que a qualidade em procedimentos m?dicos faz parte integrante do dia a dia nas estruturas hospitalares. Contudo, neste caso, a popula??o em estudo n?o pode ser considerada homog?nea como mercadorias em uma linha de produ??o. A aplica??o do CEP na ?rea de sa?de precisa admitir a exist?ncia de heterogeneidade, levando em considera??o caracter?sticas particulares de cada paciente como, por exemplo, sexo, idade, al?m de medidas que reflitam as condi??es gerais de sa?de. Neste sentido, alguns autores prop?em o monitoramento do tempo de sobreviv?ncia de pacientes, atrav?s de um gr?fico de controle de Somas Acumuladas - CUSUM (Cumulative Sum), ajustado para incorporar o risco de cada indiv?duo por meio de um modelo param?trico de regress?o. Este gr?fico ? chamado pelos autores de Gr?fico de Controle CUSUM Ajustado ao Risco (RAST CUSUM), e os resultados te?ricos foram desenvolvidos para monitorar tempos at? a ocorr?ncia de um evento, mesmo quando estes est?o sujeito a informa??es incompletas (censura ? direita), atrav?s do modelo Weibull, muito comum na modelagem de dados de sobreviv?ncia. Neste trabalho o gr?fico RAST CUSUM Weibull foi empregado com o objetivo geral de monitorar, em um estudo retrospectivo, os tempos de sobreviv?ncia dos pacientes diagnosticados com c?ncer de boca e orofaringe da Liga Norte Riograndense Contra o C?ncer no per?odo de 2001 a 2013. Os dados deste trabalho s?o oriundos da base de dados da Liga sendo registradas al?m das datas do diagn?stico e da ?ltima consulta na institui??o, caracter?sticas s?cio demogr?ficas e cl?nicas dos pacientes, consideradas como potenciais fatores de risco para redu??o no tempo m?dio de sobreviv?ncia desses indiv?duos. Como resultado do monitoramento, que levou em considera??o os fatores localiza??o e tamanho do tumor, tipo do primeiro tratamento adotado e hist?rico familiar, foi detectado um aumento de 20 % no tempo m?dio de sobreviv?ncia dos pacientes a partir do ano 2005. / Statistical process control techniques are commonly used in industrials problems and can also be applied in the health area, since quality in medical procedures are an integral part of day-to-day hospital structures. However, in this case, the population can not be regarded as homogeneous as goods in a production line. The application of the CEP in the health area must admit the existence of heterogeneity, taking into account particular characteristics of each patient with, for example, gender, age, and measures that reflect the general health conditions. In this sense, some authors propose monitoring survival time of patients, through a CUSUM (Cumulative Sum) chart, adjusted to incorporate the risk of each individual by a parametric regression model. This chart is called by the authors of RAST CUSUM, and the theoretical results were developed to monitor times until the occurrence of an event, even when they are subject to incomplete information (right censoring), considering for that the Weibull model, which is very used to model survival data. In this work the RAST CUSUM Weibull graph was used in a retrospective study, to monitoring the survival of patients with oral and oropharyngeal cancer, treated on the Northern Riograndense League of the c?ncer, from 2001 to 2013. The data of this work come from the data base of the hospital being registered beyond the dates of diagnosis and last visit to the institution, socio-demographic characteristics and clinics of patients, considered as potential risk factors for mean survival time of these individuals. As a result of the monitoring that considered the location and size of the tumor, type of the first treatment adopted and family history as risk factors, an increase of 20% in the mean survival time of the patients from 2005 was detected.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-03-21T11:50:05Z No. of bitstreams: 1 AndreiaFerreiraDoCarmo_TESE.pdf: 2170675 bytes, checksum: 49a8001d1651a77b1ebea9cb5d0cc766 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-03-23T11:55:44Z (GMT) No. of bitstreams: 1 AndreiaFerreiraDoCarmo_TESE.pdf: 2170675 bytes, checksum: 49a8001d1651a77b1ebea9cb5d0cc766 (MD5) / Made available in DSpace on 2018-03-23T11:55:44Z (GMT). No. of bitstreams: 1 AndreiaFerreiraDoCarmo_TESE.pdf: 2170675 bytes, checksum: 49a8001d1651a77b1ebea9cb5d0cc766 (MD5) Previous issue date: 2017-12-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O horm?nio glicoproteico estaniocalcina 2 (STC2) est? envolvido na carcinog?nese e progress?o de muitos tipos de c?ncer. No entanto, seu significado cl?nico e mecanismos moleculares no carcinoma de c?lulas escamosas oral (CCEO) foram pouco estudados e permanecem incertos. O presente estudo investigou associa??es da express?o da STC2 com par?metros clinicopatol?gicos e de sobrevida em pacientes com CCEO. Al?m disso, foram avaliados os efeitos biol?gicos causados pela redu??o dos n?veis de STC2 em linhagens celulares de CCEO e fibroblastos associados ao c?ncer (do ingl?s CAF ? carcinoma associated fibroblasts). A an?lise imunoistoqu?mica em 100 casos de CCEOs prim?rios indicou que a superexpress?o da STC2 foi associada com o par?metro N do sistema TNM e foi um fator de risco independente para sobrevida espec?fica da doen?a e sobrevida livre de doen?a em pacientes com CCEO. Usando ensaios in vitro, foi demonstrado que o silenciamento da STC2 em linhagens de CCEO promoveu a apoptose e reduziu a prolifera??o celular, migra??o, invas?o e transi??o epit?lio-mesenquimal. An?lises adicionais revelaram que o CAF expressa maiores n?veis de STC2 do que as c?lulas de CCEO. O silenciamento da STC2 no CAF reduziu a invas?o celular do CCEO, sugerindo que a STC2 liberada por CAFs contribui para um fen?tipo mais invasivo no CCEO. Esses resultados sugerem que a STC2 modula eventos importantes para a tumorig?nese oral e pode ser um biomarcador progn?stico para pacientes com CCEO. / The glycoprotein hormone stanniocalcin 2 (STC2) is involved in carcinogenesis and progression of several cancer types. However, its clinical significance and molecular mechanisms in oral squamous cell carcinoma (OSCC) have been partially studied and remain uncertain. In the present study, we investigated associations of STC2 expression with clinicopathological and survival parameters of OSCCs patients. We also determined the biological effects caused by STC2 downregulation in OSCC and cancer associated fibroblasts (CAF) cell lines. Immunohistochemical analysis in 100 cases of primary OSCC indicated that STC2 overexpression was associated with N stage (TNM staging) and was an independent risk factor for disease-specific survival and disease-free survival in patients with OSCC. Using in vitro assays, we demonstrated that STC2 knockdown in OSCC cell lines promoted apoptosis, and reduced cell proliferation, migration, invasion and epithelial-mesenchymal transition. Further analysis revealed that CAF expresses higher levels of STC2 than OSCC cells. Knockdown of STC2 in CAF reduced OSCC cell invasion, suggesting that STC2 released by CAF contributes to a more invasive phenotype in OSCC. These results suggest that STC2 modulates important events for oral tumorigenesis and can be a prognostic biomarker for OSCC.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-10-27T00:21:29Z No. of bitstreams: 1 NayaraPriscilaDantasDeOliveira_DISSERT.pdf: 1338360 bytes, checksum: e4a924e9aed2ef9db758540f32e2a83f (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-12-27T20:05:19Z (GMT) No. of bitstreams: 1 NayaraPriscilaDantasDeOliveira_DISSERT.pdf: 1338360 bytes, checksum: e4a924e9aed2ef9db758540f32e2a83f (MD5) / Made available in DSpace on 2016-12-27T20:05:19Z (GMT). No. of bitstreams: 1 NayaraPriscilaDantasDeOliveira_DISSERT.pdf: 1338360 bytes, checksum: e4a924e9aed2ef9db758540f32e2a83f (MD5) Previous issue date: 2016-04-20 / O c?ncer de mama apresenta altas taxas de incid?ncia e atualmente observa-se aumento consider?vel na taxa de sobrevida, de modo que a qualidade desta sobreviv?ncia passa a ser considerada uma importante quest?o de sa?de p?blica. O objetivo do estudo foi verificar a preval?ncia de incapacidade funcional e seus fatores associados em mulheres sobreviventes ao tratamento do c?ncer de mama. Trata-se de um estudo transversal, realizado com 101 mulheres residentes no munic?pio de Natal-RN com diagn?stico de neoplasia maligna da mama, que foram submetidas ao tratamento oncol?gico h? no m?nimo um ano e que ainda permanecem em acompanhamento cl?nico na Liga Norte Riograndense contra o C?ncer. O estudo foi composto por duas fases de coleta de dados, realizadas com o acesso aos prontu?rios das pacientes e com entrevistas individuais. A capacidade funcional foi aferida por meio do instrumento Disabilities of the Arm and Shoulder (DASH). Coletaram-se tamb?m vari?veis relacionadas ?s caracter?sticas socioecon?micas, h?bitos de vida, condi??es de sa?de, hist?rico ginecol?gico e obst?trico, caracter?sticas cl?nicas do tumor e abordagem terap?utica. A an?lise bivariada foi realizada por meio do teste teste Qui-quadrado de Pearson (Exato de Fisher). A an?lise multivariada foi feita por meio da Regress?o de Poisson com vari?ncia robusta. Considerou-se o n?vel de confian?a de 95%. A idade m?dia das mulheres inclu?das no estudo foi de 56,19 anos (?10,6), com renda m?dia mensal de 3,88 (?4,5) sal?rios m?nimos e com acesso ao servi?o de sa?de p?blico predominante (50,5%). Em sua maioria, as pacientes foram submetidas ? abordagem cir?rgica conservadora (53,5%). A preval?ncia de incapacidade funcional foi de 22,8% (IC95%: 13,9-31,6). A capacidade funcional mostrou-se associada de maneira estatisticamente significativa ? idade e ao tipo de acesso ao servi?o de sa?de. Pode-se concluir que as pacientes mais jovens sofreram maior impacto do tratamento do c?ncer de mama na funcionalidade quando comparadas ?s mulheres mais idosas. Quanto ao acesso ao servi?o de sa?de, as mulheres que receberam acompanhamento cl?nico p?blico apresentaram maior ocorr?ncia de incapacidade funcional, o que aponta para a necessidade de servi?os de sa?de mais organizados na sua rede assistencial, menos burocr?ticos e efetivamente resolutivos, minimizando os impactos do tratamento oncol?gico nas condi??es de vida e sa?de das sobreviventes do c?ncer de mama. / Breast cancer shows high incidence and mortality rates. However, a considerable increase in the survival rate is observed, so that the quality of life is now considered an important public health issue. The goal of this study is to determine the prevalence of disability and associated factors in women undergoing treatment for breast cancer. It is a cross-sectional study made with 101 women living in a city named Natal, in the state of Rio Grande do Norte, diagnosed with malignant neoplasm of the breast, who underwent cancer treatment for at least one year and still remain in clinical attendance in the Northern League against cancer. The study consisted of two phases of data collection, performed with access to the medical records of patients and individual interviews. The functional capacity was measured by a questionnaire called DASH. Variables related to socioeconomic characteristics, lifestyle, health, gynecological and obstetrical history, clinical characteristics of the tumor and therapeutic approach were also collected. The bivariate analysis was performed using Pearson's chisquare test (Fisher's Exact), calculating the prevalence ratio with an interval of confidence of 95%. The multivariate analysis was performed by Poisson regression with strong variance. It was considered the statistical significance level of 0.05. The average age of the women included in the study was 56,19 years (? 10,6), which in majority were white, married or in stable relationship, with high levels of education, with an average monthly income of 3 , 88 (? 4.5) times the minimum wage and access to the overriding public health service (50.5%). The prevalence of disability was 22,8% (95% CI 13,9 to 31,6). The ductal carcinoma was the most common diagnosis among women, affecting 78,2% of the sample. Most of the patients underwent conservative surgical approach (53,5%) with axillary approach (92,1%). Late postoperative complications were reported by 71,3% of the interviewed women. Functional capacity was associated with a statistically significant manner to the age and type of access to health services. It can be concluded that younger patients had a greater impact of the treatment of breast cancer in functionality when compared to older women. About the access to health services, women who received public clinical monitoring reported higher rates of disability, which points to the need for health services more organized in your care network, less bureaucratic and effectively resolving capacity, minimizing the impact of treatment cancer in living conditions and health of survivors of breast cancer.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-01-12T15:47:41Z No. of bitstreams: 1 EmanuellyBernardesDeOliveiraDaSilva_DISSERT.pdf: 1659174 bytes, checksum: 5200a1d5bddcb328c2b32c3aac297d85 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-01-20T14:20:37Z (GMT) No. of bitstreams: 1 EmanuellyBernardesDeOliveiraDaSilva_DISSERT.pdf: 1659174 bytes, checksum: 5200a1d5bddcb328c2b32c3aac297d85 (MD5) / Made available in DSpace on 2017-01-20T14:20:37Z (GMT). No. of bitstreams: 1 EmanuellyBernardesDeOliveiraDaSilva_DISSERT.pdf: 1659174 bytes, checksum: 5200a1d5bddcb328c2b32c3aac297d85 (MD5) Previous issue date: 2015-05-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Entre os tipos mais incidentes de c?ncer no Brasil, o c?ncer cervical ? o quarto tipo mais comum entre as mulheres, com mais de 99,7% dos casos associados ? infec??o pelo Papilomasv?rus Humano (HPV), tipos 16 e 18 principalmente. A resposta limitada dos tumores malignos ? quimioterapia convencional levou ao desenvolvimento de novas estrat?gias farmacol?gicas baseadas no conhecimento de novos alvos terap?uticos. Considerando a diversidade dos escorpi?es e serpentes da fauna brasileira, esses animais pe?onhentos possuem um potencial farmacol?gico em seu veneno, sendo de grande interesse na pesquisa e desenvolvimento de novos f?rmacos a partir do veneno bruto ou fra??es. Desta forma, frente a esses venenos abre-se uma expectativa na busca de novos compostos com a??o terap?utica no tratamento do c?ncer. O objetivo deste trabalho foi avaliar o efeito citot?xico e apopt?tico do veneno bruto do escorpi?o Tityus serrulatus (TSR) e das serpentes Bothrops jararaca (BJ) e Bothrops erythromelas (BE) em linhagens de carcinoma cervical SiHa e HeLa, tranformadas pelos HPV 16 e 18, respectivamente. Em rela??o ? viabilidade celular pelo m?todo de MTT, obteve-se citotoxicidade nas linhagens tumorais desafiadas com os venenos das serpentes BJ e BE quando comparada ao veneno TSR. De forma similar, o processo de morte celular, avaliada por citometria de fluxo, foi superior quando desafiados com BJ e BE. Portanto, os venenos Bothrops demonstraram uma a??o citot?xica e apopt?tica nas linhagens estudadas, de maneira dose tempo-resposta dependentes. O presente trabalho sugere, pela primeira vez, um papel citot?xico e apopt?tico dos venenos das serpentes B. jararaca e B. erythromelas em linhagens de carcinoma cervical. / Among the types of cancer incidents in Brazil, cervical cancer is the fourth most common type among women, with more than 99.7% of cases associated with infection by human papillomaviruses (HPV) types 16 and 18 mainly. The limited of malignant tumors to conventional chemotherapy response led to the development of new pharmacological strategies based on knowledge of new therapeutic targets. Considering the diversity of scorpions and snakes of Brazilian fauna, these poisonous animals have a pharmacological potential in their venom, being of great interest in the new drugs research and development from raw or fractions poison. Thus, faced with these poisons opens an expectation in the search for new compounds with therapeutic action in the treatment of cancer. The objective of this study was to evaluate the cytotoxic effect and apoptosis of the poisons of the scorpion Tityus serrulatus (TSR), Bothrops jararaca (BJ) and Bothrops erythromelas (BE) in cervical carcinoma cell lines SiHa and HeLa, tranform by the respective HPV 16 and 18. In relation to cell viability by MTT method was obtained challenged cytotoxicity in tumor cell lines with the poisons of snakes BJ and BE compared to the TSR poisons. Similarly, the process of cell death was greater when challenged with BJ and BE. Therefore, Bothrops poisons showed a cytotoxic and apoptotic action on the lines studied, dose-dependent and time-response manner. This study suggests for the first time, a cytotoxic and apoptotic role of poisons of snakes B. jararaca and B. erythromelas in cervical carcinoma cell lines.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-20T19:31:22Z No. of bitstreams: 1 JanineDeAraujoSilva_DISSERT.pdf: 3090092 bytes, checksum: b11ae8004e5eb556878f21976b10b352 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-21T22:51:22Z (GMT) No. of bitstreams: 1 JanineDeAraujoSilva_DISSERT.pdf: 3090092 bytes, checksum: b11ae8004e5eb556878f21976b10b352 (MD5) / Made available in DSpace on 2017-03-21T22:51:22Z (GMT). No. of bitstreams: 1 JanineDeAraujoSilva_DISSERT.pdf: 3090092 bytes, checksum: b11ae8004e5eb556878f21976b10b352 (MD5) Previous issue date: 2016-10-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O estudo para a obten??o de nanopart?culas de ouro (AuNPs) funcionalizadas por mol?culas bioativas tem se mostrado relevante no desenvolvimento de novas alternativas para tratamento do c?ncer. O presente trabalho descreve a s?ntese de AuNPs estabilizadas por dois derivados quinoxal?nicos, 2,3-dietanolaminaquinoxalina (DEQX) e 2-(2,3-di-idro-[1,4]oxazino[2,3-b]quinoxalin-4-il)etanol (OAQX), sintetizados por nosso grupo de pesquisa, verificados por possu?rem atividade antic?ncer em estudos envolvendo c?lulas. Para obten??o das AuNPs funcionalizadas com DEQX e OAQX, o glicerol foi utilizado como principal agente redutor. As AuNPs foram caracterizadas por espectroscopia ultravioleta-vis?vel (UV-Vis), infravermelho (FTIR-ATR) e microscopia eletr?nica de transmiss?o (TEM). Os resultados de UV-Vis indicaram que a melhor condi??o de s?ntese das AuNPs foi quando as propor??es de Au3+ em rela??o aos ligantes org?nicos foi de 1:0,2 e 1:1 para DEQX e OAQX, respectivamente. Nestas condi??es, foram obtidas AuNPs esf?ricas e monodispersas, com dimens?es m?dias de 9,7 e 11,5 nm quando nas estabiliza??es por DEQX e OAQX, respectivamente. O mecanismo de forma??o das part?culas foi proposto com base nos resultados experimentais, e parece envolver uma complexa??o entre Au3+ e os ligantes previamente ? forma??o das AuNPs. O fato das bandas em torno de 525 nm do espectro de UV-Vis, caracter?sticas das AuNPs, tornarem-se mais intensas e com melhor defini??o com o passar do tempo corroboram para essa hip?tese. Foi realizado um estudo relacionado ? estabilidade das AuNPs em fun??o do pH, onde foi verificado que as mesmas se mostraram relativamente est?veis em pH pr?ximo a 7,4. O ligante DEQX foi mais eficiente na estabiliza??o das AuNPs quando comparado ao OAQX. Os resultados obtidos no presente trabalho indicaram que foram obtidas AuNPs esf?ricas monodispersas funcionalizadas com duas esp?cies org?nicas bioativas, abrindo a possibilidade de explora??o destes sistemas para fins medicinais, em especial para o tratamento do c?ncer. / The study on obtainment of gold nanoparticles (AuNPs) functionalized with bioactive molecules have been shown to be relevant in the development of new alternatives for cancer treatment. This study describes the synthesis of AuNPs stabilized by two quinoxaline derivatives, 2,3- diethanolaminequinoxaline (DEQX) and 2-(2,3-di-hydro-[1,4]oxazine[2,3-b]quinoxaline-4-yl) ethanol (OAQX), synthetized by our research group, verified as possessing anticancer activity in studies involving cells. For the obtainment of AuNPs functionalized with DEQX and OAQX, glycerol was used as main reducing agent. The AuNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis), by infrared (FTIR-ATR), and by transmission electronic microscopy (TEM). The results from the UV-Vis showed that the best synthesis condition of AuNPs happened when the proportions of Au3+ in relation to organic ligands were 1:0,2 and 1:1 for DEQX and OAQX, respectively. In those given conditions, monodispersed spherical AuNPs were obtained, with average size of 9.7 and 11.5 nm when stabilized by DEQX and OAQX, respectively. The particles formation mechanism proposed was based on the experimental results, and it appears to involve a complexation between Au3+ and the referred ligands previously to the formation of the AuNPs. The fact that the bands at around 525 nm from the UV-Vis spectrum - characteristics of AuNPs - have become more intense and with better definition over time corroborate to this assumption. A study related to the stability of AuNPs according to the pH was carried out, being thus possible to verify that they showed to be relatively stable at pH near 7,4. The DEQX ligand was more efficient on the stabilization of AuNPs when compared to the OAQX ligand. The results obtained in this work indicated the formation of spherical monodisperse AuNPs functionalized with two bioactive organic species. This leads to the possibility of exploitation of these systems for medical purposes, especially for cancer treatment.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-10-05T00:28:19Z No. of bitstreams: 1 AnaKarinaSilvaCavalcantiFontela_DISSERT.pdf: 1824901 bytes, checksum: a254de7c0021428a7bf8f8b15aebb282 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-10-16T23:46:51Z (GMT) No. of bitstreams: 1 AnaKarinaSilvaCavalcantiFontela_DISSERT.pdf: 1824901 bytes, checksum: a254de7c0021428a7bf8f8b15aebb282 (MD5) / Made available in DSpace on 2017-10-16T23:46:51Z (GMT). No. of bitstreams: 1 AnaKarinaSilvaCavalcantiFontela_DISSERT.pdf: 1824901 bytes, checksum: a254de7c0021428a7bf8f8b15aebb282 (MD5) Previous issue date: 2016-08-12 / Diante da necessidade de diminuir a incid?ncia do c?ncer de colo de ?tero e as les?es causadas pelo v?rus HPV, o Minist?rio da Sa?de iniciou, em mar?o de 2014, a campanha de vacina??o contra o HPV nas meninas de 11 a 13 anos em todo o territ?rio nacional. Em virtude da procura dessas jovens adolescentes pelo servi?o da aten??o prim?ria, considerou-se importante desenvolver um estudo, cujo objetivo foi o de analisar como as usu?rias vacinadas contra o HPV, em uma Unidade de Sa?de de Recife, percebem a preven??o do c?ncer de colo uterino. Tratou-se de uma pesquisa-a??o, para cujo desenvolvimento foram constitu?dos dois grupos focais e duas oficinas. Cada grupo contou com um moderador, que conduziu a discuss?o, por meio de um roteiro-guia, com os seguintes temas: conhecimento e compreens?o sobre a infec??o pelo HPV e sua liga??o com o c?ncer do colo de ?tero; entendimentos e preocupa??es sobre a vacina??o contra o HPV; experi?ncia de vacina??o; entendimentos sobre a import?ncia do rastreio do c?ncer do colo de ?tero e conhecimento sobre as estrat?gias de preven??o do c?ncer uterino na aten??o b?sica, e com um anotador/registrador, respons?vel por gravar o ?udio e transcrever as discuss?es. A amostra foi composta de vinte meninas na faixa et?ria entre 11 e 14 anos, que j? haviam tomado a primeira dose da vacina, frequentavam a Unidade de Sa?de Professor M?rio Ramos h?, pelo menos, seis meses e eram acompanhadas pelo Programa de Agentes Comunit?rios de Sa?de. As reuni?es ocorreram em um local confort?vel e neutro, o encontro foi quinzenal, em dia e hora combinados com as adolescentes, e as participantes foram convidadas pelos agentes de sa?de que as visitaram no dia anterior para confirmar sua presen?a. Os dados foram interpretados ? luz da An?lise de Conte?do de Bardin. Conclui-se que a maioria das adolescentes sente necessidade de mais esclarecimentos sobre o HPV e sua rela??o com o c?ncer uterino. Ficou evidente que os conhecimentos a respeito do HPV e suas formas de preven??o e a vacina dispon?vel para essa faixa et?ria s?o limitados. Este estudo aponta para a import?ncia de se discutir sobre a preven??o do c?ncer uterino nas escolas p?blicas, nas privadas e nos servi?os de aten??o ? sa?de, e que ? preciso explorar com mais frequ?ncia o assunto na esfera da sexualidade. H? que se ressaltar que ? imprescind?vel conhecer e redimensionar as dificuldades das jovens e propor uma reflex?o sobre o autoconhecimento, esclarecendo d?vidas sobre os procedimentos cl?nicos, compartilhar experi?ncias com outras jovens, criar v?nculos com os profissionais de sa?de e aderir ?s pr?ticas participativas. Espera-se que, atrav?s dessas a??es, diminua-se o n?mero de casos e de ?bitos causados pelo HPV. / Faced with the need to reduce the incidence of cervical cancer and lesions caused by the HPV virus the Ministry of Health began in March 2014 the vaccination campaign against HPV in girls 11-13 years throughout the country, because that the demand for such young adolescents at the service of primary care has become important to develop a study, which the research objective to analyze the perception on the prevention of cervical cancer in vaccinated users against HPV in a Recife Health Unit. This is an action research in which there will be two focus groups and a workshop. Each group will consist of 01 moderator leading the discussion by a road map to guide the following themes: knowledge and understanding of HPV infection and its link to cervical cancer; understandings and concerns about HPV vaccination; vaccination experiments; understandings about the importance of cervical cancer screening and knowledge on strategies to prevent cancer of the cervix in primary care, 01 recorder / registrar responsible for recording with audio and transcribe the discussions minimum of five and maximum number 10 girls aged between 11 and 14 years, who have already taken the first dose of the vaccine, attending the Health Unit Professor Mario Ramos for at least six months and is accompanied by the Community Health Agents Program. The meetings will take place in a comfortable and neutral location, the meetings will be held monthly on day and time combined with the teenagers, and participants will be invited by CHWs who had visited the day before confirming their presence. Data analysis will be conducted through Bardin Content Analysis. It is concluded that the majority of adolescents feel the need for further clarification on HPV and its relation to uterine cancer. It was clear that knowledge about HPV and its forms of prevention and available vaccine for this age range are limited. This study points to the importance of discussing the prevention of uterine cancer in public schools, private schools and health care services, and that the issue of sexuality must be explored more frequently. It is necessary to emphasize that it is essential to know and resize the difficulties of young people and to propose a reflection on self-knowledge, clarifying doubts about clinical procedures, sharing experiences with other young people, creating links with health professionals and adhering to participatory practices. It is hoped that, through these actions, the number of cases and deaths caused by HPV will decrease.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-10-18T20:17:23Z No. of bitstreams: 1 AnaCarolinaDeOliveiraNevesMenezes_TESE.pdf: 10687959 bytes, checksum: ac1430b19dc8e137155eb42a1171c56b (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-10-19T20:52:18Z (GMT) No. of bitstreams: 1 AnaCarolinaDeOliveiraNevesMenezes_TESE.pdf: 10687959 bytes, checksum: ac1430b19dc8e137155eb42a1171c56b (MD5) / Made available in DSpace on 2017-10-19T20:52:18Z (GMT). No. of bitstreams: 1 AnaCarolinaDeOliveiraNevesMenezes_TESE.pdf: 10687959 bytes, checksum: ac1430b19dc8e137155eb42a1171c56b (MD5) Previous issue date: 2017-08-09 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Esta tese relata a aplica??o das espectroscopias no infravermelho m?dio, de fluoresc?ncia molecular e espectrometria de massas, combinadas a t?cnicas de an?lise multivariada, para classifica??o de c?lulas cancerosas em cultivo e de les?es pr?-cancerosas atrav?s de plasma sangu?neo. Em um primeiro estudo, matrizes de excita??o/emiss?o de fluoresc?ncia molecular foram obtidas para diferentes linhagens de c?lulas normais (3T3, ARPE, HEK) e cancerosas (HepG2, HeLa, HT-29, 786-0) e modelos de classifica??o foram constru?dos utilizando uma combina??o dos algoritmos OPLS e UPLS-DA. Taxas de acerto de 100% e 75% foram obtidas para as classes Normal e Cancerosa, respectivamente. Ainda, foi avaliada a influ?ncia dos anticorpos anti-MMP-2 e anti-MMP-9 no desempenho dos modelos de classifica??o. Na presen?a dos anticorpos, as taxas de acerto nas classifica??es aumentaram consideravelmente atingindo 100% para ambas as classes, Normal e Cancerosa, atrav?s dos algoritmos OPLS/UPLS-DA. Em um segundo estudo, a espectroscopia ATR-FTIR foi utilizada para obten??o de espectros de plasmas sangu?neos de mulheres saud?veis (negativas para les?o intraepitelial ou malignidade, NILM) e portadoras de les?o intraepitelial cervical (SIL) de baixo (LSIL) ou alto grau (HSIL), causadas pelo v?rus HPV. Modelos multivariados de classifica??o foram constru?dos, visando uma metodologia de rastreamento para o c?ncer cervical. Os algoritmos PCA-LDA/QDA, SPA-LDA/QDA e GA-LDA/QDA foram aplicados como ferramentas de classifica??o e seus desempenhos comparados. De maneira geral, os resultados obtidos atrav?s do algoritmo GA-QDA foram os mais satisfat?rios, utilizando apenas vari?veis espectrais selecionadas que puderam ser relacionadas a grupos funcionais pertencentes a diferentes biomol?culas. Os modelos GA-QDA classificaram corretamente NILM vs. SIL com sensibilidade e especificidade em torno de 90% e 83%, respectivamente. NILM vs. LSIL apresentaram sensibilidade e especificidade variando entre 67-94% e 82-94%, respectivamente. Para NILM vs. HSIL, os valores de sensibilidade e especificidade estiveram entre 76-97% e 73-100%, respectivamente. Em um terceiro estudo, a espectrometria de massas foi aplicada para obter os espectros de lip?dios extra?dos do plasma sangu?neo de mulheres da Classe NILM (n=42) e SIL (n=34). Modelos de classifica??o multivariados foram constru?dos utilizando os classificadores LDA, QDA e SVM. Os modelos baseados em SVM permitiram a discrimina??o das classes com sensibilidade e especificidade de 83.3% e 80.0% para NILM e SIL, respectivamente. Alguns poss?veis lip?dios foram associados a cada classe, tais como prostaglandinas, esfingolip?dios e fosfolip?dios, Tetranor-PGFM e um lip?dio hidroxiperoxidado. Os resultados obtidos em todos os estudos evidenciam a potencialidade das t?cnicas espectrosc?picas e multivariadas como poss?veis metodologias de rastreamento e identifica??o de c?ncer, o que poderia contribuir fortemente para a redu??o da morbidade e mortalidade causadas pela doen?a. / This thesis reports the application of both infrared and molecular fluorescence spectroscopy, as well as mass spectrometry, combined with multivariate analysis techniques for classification of cancerous cells in culture medium and precancerous lesions in blood plasma. In a first study, excitation/emission matrices of molecular fluorescence were obtained for normal (3T3, ARPE, HEK) and cancerous (HepG2, HeLa, HT-29, 786-0) cell lines and classification models were built by using a combination of the algorithms OPLS and UPLS-DA. Correct classification indexes of 100% and 75% were obtained for both classes, Normal and Cancer, respectively. In addition, it was evaluated the influence of the antibodies anti-MMP-2 and anti-MMP-9 in the performance of the classification models. In the presence of the antibodies, the correct classification indexes were considerably improved reaching 100% for both classes, Normal and Cancer, using the algorithms OPLS/UPLS-DA. In a second study, the ATR-FTIR spectroscopy was applied to obtain the spectra of blood plasma of both healthy women (negative for intraepithelial lesion or malignancy, NILM) and women with cervical intraepithelial lesion (SIL) of low grade (LSIL) or high grade (HSIL), caused by HPV virus. Multivariate classification models were built, aiming a screening methodology for cervical cancer. The algorithms PCA-LDA/QDA, SPA-LDA/QDA and GA-LDA/QDA were applied as classification tools and their performance was evaluated. In general, the results obtained by GA-QDA were the most satisfactory, by using only chosen spectral variables that could be related to chemical groups of different biomolecules. The models GA-QDA correctly classified NILM vs. SIL with sensitivity and specificity around 67-94% e 82-94%, respectively. For NILM vs. LSIL, sensitivity and specificity values were about 67-94% e 82-94%, respectively. For NILM vs. HSIL, the sensitivity and specificity values were 76-97% e 73-100%, respectively. In the third study, mass spectrometry was applied to obtain the spectra of lipids extracted from blood plasma of women of NILM (n=42) and SIL (n=34) classes. Multivariate classification models were built by using the classifiers LDA, QDA and SVM. SVM-based models allowed to discriminate the classes with sensitivity and specificity values of 83.3% and 80.0% for NILM and SIL, respectively. Some possible lipids were associated to each class, such as prostaglandins, phospholipids, sphingolipids, Tetranor-PGFM and a hydroperoxide lipid. The results achieved in all studies highlight the potentiality of the spectroscopic and multivariate techniques as possible methodologies for cancer screening, what could effectively contribute to reduce morbidity and mortality caused by cancer.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2017-10-03T13:27:09Z No. of bitstreams: 1 MARIA_NOEL__MARZANO_RODRIGUES_PETRUZZI_TES.pdf: 3641098 bytes, checksum: 3aa8ce331659fd0a8d765be0dc6f3d45 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-10-04T12:34:26Z (GMT) No. of bitstreams: 1 MARIA_NOEL__MARZANO_RODRIGUES_PETRUZZI_TES.pdf: 3641098 bytes, checksum: 3aa8ce331659fd0a8d765be0dc6f3d45 (MD5) / Made available in DSpace on 2017-10-04T12:36:41Z (GMT). No. of bitstreams: 1 MARIA_NOEL__MARZANO_RODRIGUES_PETRUZZI_TES.pdf: 3641098 bytes, checksum: 3aa8ce331659fd0a8d765be0dc6f3d45 (MD5) Previous issue date: 2017-06-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / A late diagnosis of oral squamous cell carcinoma is related to high morbidity and mortality rates, as well as recurrence after treatment. Hence, there is an increasing interest in the validation of biological markers, chemoprevention strategies, and adjuvant treatment alternatives for combating oral cancer. The present Thesis evaluated the anticancer effect of cannabidiol (CBD) in a validated experimental murine model of oral carcinogenesis. Fifteen Fischer 344 rats were randomly divided into three groups of five animals each and subjected to a 7,12-dimethylbenzanthracene (DMBA) topical application on the ventral mucosa of their tongues, thrice a week. From the 2nd to the 12th week, they received intraperitoneally-administered vehicle (group 1), CBD 5 mg/kg (group 2), or CBD 10 mg/kg (group 3). After euthanasia, the tongues were dissected, processed and assessed by histological and immunohistochemical analysis. Group 2 and group 3 showed inhibition of severe oral epithelial dysplasia and carcinoma, and exhibited lower cell proliferation as compared to group 1. The null hypothesis was rejected when results showed statistical significance at a 0.05 level (confidence interval = 95%). In sequence are presented three manuscripts, first one regarding the original experiment and the two subsequent ones providing overall theoretical support. / O diagn?stico tardio do carcinoma espinocelular oral est? relacionado a um alto ?ndice de morbi-mortalidade e recorr?ncia ap?s o tratamento. Portanto, h? um crescente interesse na valida??o de marcadores biol?gicos que contribuam para o diagn?stico precoce, novas estrat?gias de quimiopreven??o e recursos adjuvantes para o tratamento dessa neoplasia maligna. A presente Tese centrou-se na avalia??o do efeito anticarcinog?nico do canabidiol (CBD) em um modelo experimental consagrado para a indu??o de altera??es epiteliais, com risco de malignidade, na mucosa oral de murinos. Para tanto, 15 ratos Fischer 344 foram aleatoriamente divididos em tr?s grupos, onde todos os animais tiveram o ventre de suas l?nguas expostas ao 7,12-dimetilbenzantraceno (DMBA) tr?s vezes por semana. A partir da segunda semana os grupos receberam por via intraperitoneal, ve?culo (grupo 1), 5 mg/Kg ou 10 mg/Kg de CBD (grupos 2 e 3, respectivamente). Na d?cima segunda semana ap?s o in?cio do experimento realizou-se a eutan?sia dos animais, dissec??o das l?nguas e processamento dos esp?cimes. As an?lises foram realizadas por meio das t?cnicas histol?gicas de rotina e imunoistoqu?mica. Observou-se a inibi??o do desenvolvimento de displasia oral severa e carcinoma, bem como a modula??o dos ?ndices de prolifera??o celular nos grupos 2 e 3 em rela??o ao grupo 1. A hip?tese nula p?de ser rejeitada, uma vez que os resultados obtidos apresentaram n?vel de signific?ncia de 0,05 (intervalo de confian?a = 95%). A seguir, apresentam-se tr?s artigos cient?ficos que descrevem primeiramente o experimento original desenvolvido e, em sequ?ncia, as revis?es da literatura para subsidiar a discuss?o do tema proposto.

Made available in DSpace on 2016-04-04T18:27:31Z (GMT). No. of bitstreams: 1 Marcela Fileti.pdf: 341605 bytes, checksum: 22b6ac71b02c0368db63564346f9cb49 (MD5) Previous issue date: 2007-02-26 / Pontif?cia Universidade Cat?lica de Campinas / Cancer is among the most important causes of death in Brazil and around the world. The most common type to origin death in women is the breast cancer. This study intended to analyze the possible contingences that people with breast cancer were exposed before the beginning of the disease in order to understand the relationship between the behavior and development of cancer. Participated of this study four women with primary diagnosis of breast neoplasm, without history breast cancer in their family (mother and/or sister), age between 40 and 51, that have had children and with medical following in an Oncology s Ambulatory of a general hospital. The theory selected was the Behavior Analysis, based on Radical Behaviorism Philosophy. Considering the data obtained by the relates of the participants during the clinical interview, it was done the analysis of behaviors that presented relevant in previous period of the disease establishment, that had variation of 4 to 8 years depending on the medical diagnosis to each participant. The results indicated that there is a common behavior style of social reinforce strong rules, behaviors maintained by negative reinforcement, lack of repertory to identify the uncovered behaviors and their aversive consequences and absence of selfknowledge. The exposition on aversive events and the reduced repertory of escape s behavior suggest a poor repertory in order to work with aversive life events, that would contribute to an organism s vulnerability and possible development of neoplasm. The results may suggest that behavioral standard maybe have link with the development of diseases like the breast cancer. Other surveys in Analysis of Behavior area relating contingences and diseases must be conduced. / O c?ncer figura entre as principais causas de morte no Brasil e no mundo. O tipo mais comum a provocar mortes em mulheres ? o c?ncer de mama. Esta pesquisa objetivou analisar as prov?veis conting?ncias a que pessoas portadoras de c?ncer de mama estiveram expostas antes do in?cio da doen?a, a fim de compreender a rela??o entre o comportamento e o desenvolvimento do c?ncer. Participaram do estudo 4 mulheres com diagn?stico prim?rio de neoplasia maligna de mama, sem hist?ria da doen?a na fam?lia (m?e e/ou irm?), com idade entre 40 e 51 anos, com filhos, que encontravam-se em acompanhamento m?dico em um ambulat?rio de oncologia de um hospital geral. A abordagem te?rica selecionada foi a An?lise do Comportamento, baseada na filosofia do Behaviorismo Radical. Por meio dos dados obtidos pelos relatos das participantes durante a entrevista cl?nica, fez-se a an?lise dos comportamentos que se mostraram relevantes na ?poca anterior ao in?cio da doen?a, que variou de 4 a 8 anos dependendo do diagn?stico m?dico para cada participante. Os resultados indicaram um padr?o comportamental comum de presen?a de auto-regras r?gidas refor?adas socialmente, comportamentos mantidos por refor?amento negativo, falta de repert?rio para identifica??o de comportamentos encobertos e as conseq??ncias aversivas desses comportamentos, aus?ncia de autoconhecimento. A exposi??o aos eventos aversivos e o baixo repert?rio de comportamentos de fuga sugerem um pobre repert?rio para lidar com eventos aversivos de vida, o que colaboraria para a vulnerabilidade do organismo e poss?vel desenvolvimento da neoplasia. Os resultados permitem sugerir que padr?es comportamentais possivelmente relacionamse com o desenvolvimento de doen?as como o c?ncer de mama. Outras pesquisas na ?rea da An?lise do Comportamento relacionando conting?ncias e doen?as devem ser conduzidas.

Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-04-24T12:41:11Z No. of bitstreams: 1 ShirleyPontesDissertacao2018.pdf: 1632850 bytes, checksum: 1119ac63b939927d264497f421422810 (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-04-24T12:41:31Z (GMT) No. of bitstreams: 1 ShirleyPontesDissertacao2018.pdf: 1632850 bytes, checksum: 1119ac63b939927d264497f421422810 (MD5) / Made available in DSpace on 2018-04-24T12:41:31Z (GMT). No. of bitstreams: 1 ShirleyPontesDissertacao2018.pdf: 1632850 bytes, checksum: 1119ac63b939927d264497f421422810 (MD5) Previous issue date: 2018-02-23 / The diagnosis and treatment of prostate cancer can cause significant changes in the life of men influencing their quality of life. The objective of this study was to evaluate the health-related quality of life of patients with prostate cancer submitted to radiotherapy. It is a descriptive cross-sectional study with non-probabilistic sampling. Subjects eligible for the study were 120 men with prostate cancer who underwent on treatments at HUB - CACON. The instruments used were EORTC QLQ-C30 and the International Index Of Erectil Function- IIEF-5. The research project was approved by the Ethics and Research Committee of the Faculty of Medicine of the Catholic University of Brasilia under the number CAAE: 58197916.8.0000.0029.: The profile os subjects is aged between 70 and 79 years (42.3%), with elementary school (72.3%), brown (44.6%), married or had a partner (75.6%). Among the comorbidities, the highest incidence was arterial hypertension (70%) followed by Diabetes MellitusII (33.8%). The most frequent toxicities resulting from RT treatment, was dysuria (43%), followed by diarrhea (22.3%). The analysis of IIEF-5 allows to observe that 79.2% suffer from severe erectile dysfunction.. The prostate cancer treatments tend to cause negative impacts on sexual function and self-perceived quality of life. The age does not influence role performance, and isolated or simultaneous treatments proved to be important factor to erectile function score. Conclusion: The group who have undergone to RT + RP suffer more from pain and fatigue, and the group who have undergone to RT + CQ suffer more insomnia. All participants who have undergone RT + PR + CQ suffer severe erectile dysfunction. Considering the findings of this study, it confirm that the patients with prostate cancer who manage maintain erectile function moderate, mild and normal, they as well maintain their social roles as work, family relationships and marriage. They also keep their emotions balanced and control their irritability, tension, depression, and they feel less fatigue. This study allows to understand the local reality and corroborates with the findings of other studies carried out in different regions and social contexts. / O diagn??stico e o tratamento do c??ncer de pr??stata podem provocar mudan??as significativas na vida dos homens e influenciar a sua qualidade de vida. O objetivo deste estudo foi avaliar a qualidade de vida relacionada ?? sa??de e a fun????o er??til dos pacientes com c??ncer de pr??stata. Trata-se de um estudo descritivo de corte transversal com amostragem n??o probabil??stica. Os indiv??duos eleg??veis para o estudo foram 120 homens portadores de c??ncer de pr??stata, que se submeteram ?? radioterapia no HUB ??? CACON. Os instrumentos utilizados foram o EORTC QLQ-C30, e o ??ndice Internacional de Fun????o Er??til IIEF-5. O projeto de pesquisa foi aprovado pelo Comit?? de ??tica e Pesquisa da Faculdade de Medicina da Universidade Cat??lica de Bras??lia sob o n??mero CAAE: 58197916.8.0000.0029. O perfil do participante ?? idade entre 70 a 79 anos (42,3%), com ensino fundamental (72,3%), da cor parda (44,6%), casado ou tinha uma companheira (75,6%). Dentre as comorbidades a de maior incid??ncia foi hipertens??o arterial (70%) seguida de Diabetes Mellitus tipo 2 (33,8%). As toxicidades decorrentes do tratamento com RT, a mais frequente foi dis??ria (43%), seguida por diarreia (22,3%). A an??lise do IIEF- 5 permite observar o ??ndice de disfun????o er??til dos participantes, em que 79,2% dos participantes sofrem de disfun????o er??til severa. Discuss??o: Os tratamentos de c??ncer de pr??stata tendem a causar impactos negativos na fun????o sexual. E na autopercep????o da qualidade de vida. A idade n??o influencia no desempenho de papeis sociais. Os tratamentos se realizados isolados ou simultaneamente provaram ser um importante fator no escore da fun????o er??til. Conclus??o: O grupo que se submeteu a RT + RP sofre mais de dor e fadiga, o grupo que se submeteu a RT + CQ sofre mais de ins??nia e todos os participantes que se submeteram a RT + PR + CQ sofrem de disfun????o er??til severa. Considerando os achados desse estudo, se confirma que pacientes com c??ncer de pr??stata que conseguem manter a fun????o er??til entre moderada, leve e normal, tamb??m mant??m os desempenho de papeis sociais como trabalho, fam??lia, relacionamentos e o casamento. Eles tamb??m conseguem manter as emo????es mais equilibradas como controle da irritabilidade, tens??o e depress??o, e sentem menos fadiga.. Este estudo permite entender a realidade local bem como corrobora os achados de outros estudos em diferentes regi??es e contextos sociais.