La réaction du greffon contre l’hôte (GvH) est responsable d’un grand taux de morbidité et de mortalité chez les patients recevant des greffes de cellules souches (GCSH) allogéniques. Dans ce contexte, les cellules T régulatrices sont largement étudiées et semblent avoir un grand potentiel d’utilisation dans le domaine de la thérapie cellulaire de la GvH. Parmi les populations cellulaires T régulatrices, les lymphocytes T CD4-CD8- TCRαβ+ « Doubles-Négatifs » (DN), qui ne représentent que 1-3% des lymphocytes T, ont été décrits. Ces cellules ont des propriétés inhibitrices de la réponse immunitaire qui s’avèrent spécifiques aux antigènes auxquels elles ont préalablement été exposées. La répression de la réponse immunitaire par les cellules T DN régulatrices semble être un mécanisme important impliqué dans l’induction de la tolérance aux allo-antigènes. De plus, ces cellules confèrent une tolérance immunitaire dans des modèles de greffes allogéniques et xénogéniques. En effet, ces cellules ont la capacité d’inhiber la réaction contre un allo-antigène auquel elles ont été exposées, sans inhiber la réaction contre un allo-antigène inconnu. Les cellules T DN ont été isolées et caractérisées chez l’homme où elles ont la capacité d’interagir avec des cellules présentatrices d’antigènes (APCs) par un contact cellulaire, comme chez la souris. Cependant, leur capacité immunomodulatrice reste inconnue chez l’humain. Notre objectif consistait donc principalement à étudier le rôle et le mécanisme d’action des cellules T DN régulatrices humaines in vitro, en étudiant leur capacité à inhiber une réaction lymphocytaire mixte (MLR). Nous avons montré que les cellules T DN stimulées par un allo-antigène donné inhibent des cellules syngéniques effectrices dirigées contre ce même alloantigène mais n’inhibent pas des cellules syngéniques effectrices dirigées contre un autre alloantigène, démontrant ainsi la spécificité aux antigènes de ces cellules. De plus, les T DN non stimulées par un allo-antigène n’ont pas de rôle inhibiteur. Cependant, durant cette inhibition, nous n’observons pas de modulation de l’expression des marqueurs d’activation et d’induction de l’apoptose. Afin d’étudier le mécanisme d’action des cellules T DN, nous avons mesuré l’expression intracellulaire de la granzyme B. Les résultats démontrent que les cellules T DN stimulées expriment un niveau significativement plus élevé de granzyme B que les cellules T DN non-stimulées par l’allo-antigène. Ceci suggère que l’immunosuppression induite par les cellules T DN stimulées pourrait passer par la voie granzyme B. Le mécanisme utilisé par ces cellules reste à être confirmé par nos futures expériences. / Graft-versus-Host Disease (GvHD) is a major cause of morbidity and mortality in patients receiving an allogeneic Hematopoietic Stem Cell Transplantation (HSCT). Many regulatory T cell populations have been studied and shown to have immunosuppressive properties in GvHD. Among these populations, Double Negative CD4-CD8-TCRαβ+ regulatory T cells (DN T) have been described. These cells represent 1-3% of all T cell lymphocytes and are known to have antigen-specific inhibitory functions of the immune response. The suppression of an immune response by DN T cells seems to be an important mechanism involved in the induction of tolerance to allo-antigens. Moreover, these cells also confer immune tolerance in models of allogeneic and xenogenic grafts. DN T cells have the ability to suppress syngeneic T CD4+ and T CD8+ cells in an antigen-specific manner. Therefore, these DN T cells can inhibit the reaction caused by syngeneic effector cells against a specific alloantigen to which they have been previously exposed. However, they cannot inhibit a reaction directed against an unknown alloantigen. Human DN T cells have been isolated and characterized as cells that have the capacity to interact with APCs by cell-to-cell contact, just like in mice. However, their immunomodulatory properties are still unknown in humans. The goal of our project was to study the role and immunomodulatory functions of human DN T cells in Mixed Lymphocyte Reactions (MLR). The MLRs have allowed us to demonstrate that DN T cells, after having been stimulated by an allo-antigen, have an antigen-specific inhibitory function towards the syngeneic effector cells reacting against the same alloantigen that they have been exposed to. Interestingly, they do not inhibit the reaction of these effector cells against an unknown alloantigen. However, stimulated DN T cells did not modulate the expression of the activation markers expressed by the effector cells and did not give a death signal to these cells either. Moreover, we also wanted to study how DN T cells have an immunosuppressive activity. Therefore, we compared the expression of Granzyme B in stimulated versus non-stimulated cells. Our results suggest that DN T cells may use the Granzyme B pathway to immunosuppress the effector cells. In conclusion, our results demonstrate that DN T cells have an antigen-specific inhibitory function. The mechanism used by these DN T cells remains to be confirmed with our future experiments.

No description available.

The purpose of this paper is to examine how LKAB are portrayed in the newspaper Dagens Nyheter (DN). The methods that were used were: Critical Discourse Analysis (CDA) combined with Critical Linguistic Analysis. These were used since they help to reveal the structures and unconscious meaning and power of word. It gives the tools to see how authority, power and meaning of words are constructed during social practice of the way they are used. To complement the method the theories that’s used are discourse analysis since CDA is both a method and a theory. A theory concerning journalism has also been included for the paper.As a result of the analysis, it shows that LKAB is portrayed as a large company with huge amount of economic resources. In articles with a positive attitude against the company they are regarded as a strong and active company in the Swedish economy during the financial crisis that generates a lot of money for the Swedish state. Articles that portrait LKAB in a negative way, regards them as a company that with the power of the state are allowed to look past the individuals that lives near the mines or the environment as long as it generates money for them and the state. The people living near the mines will have to accept the thing that’s happening to them. This paper also shows that the articles that were analyzed, all of them confirm and legitimize the power to certain sources used for facts which confirms that journalists needs certain sources to appear as secure but that also let the sources with power keep their power.

La réaction du greffon contre l’hôte (GvH) est responsable d’un grand taux de morbidité et de mortalité chez les patients recevant des greffes de cellules souches (GCSH) allogéniques. Dans ce contexte, les cellules T régulatrices sont largement étudiées et semblent avoir un grand potentiel d’utilisation dans le domaine de la thérapie cellulaire de la GvH. Parmi les populations cellulaires T régulatrices, les lymphocytes T CD4-CD8- TCRαβ+ « Doubles-Négatifs » (DN), qui ne représentent que 1-3% des lymphocytes T, ont été décrits. Ces cellules ont des propriétés inhibitrices de la réponse immunitaire qui s’avèrent spécifiques aux antigènes auxquels elles ont préalablement été exposées. La répression de la réponse immunitaire par les cellules T DN régulatrices semble être un mécanisme important impliqué dans l’induction de la tolérance aux allo-antigènes. De plus, ces cellules confèrent une tolérance immunitaire dans des modèles de greffes allogéniques et xénogéniques. En effet, ces cellules ont la capacité d’inhiber la réaction contre un allo-antigène auquel elles ont été exposées, sans inhiber la réaction contre un allo-antigène inconnu. Les cellules T DN ont été isolées et caractérisées chez l’homme où elles ont la capacité d’interagir avec des cellules présentatrices d’antigènes (APCs) par un contact cellulaire, comme chez la souris. Cependant, leur capacité immunomodulatrice reste inconnue chez l’humain. Notre objectif consistait donc principalement à étudier le rôle et le mécanisme d’action des cellules T DN régulatrices humaines in vitro, en étudiant leur capacité à inhiber une réaction lymphocytaire mixte (MLR). Nous avons montré que les cellules T DN stimulées par un allo-antigène donné inhibent des cellules syngéniques effectrices dirigées contre ce même alloantigène mais n’inhibent pas des cellules syngéniques effectrices dirigées contre un autre alloantigène, démontrant ainsi la spécificité aux antigènes de ces cellules. De plus, les T DN non stimulées par un allo-antigène n’ont pas de rôle inhibiteur. Cependant, durant cette inhibition, nous n’observons pas de modulation de l’expression des marqueurs d’activation et d’induction de l’apoptose. Afin d’étudier le mécanisme d’action des cellules T DN, nous avons mesuré l’expression intracellulaire de la granzyme B. Les résultats démontrent que les cellules T DN stimulées expriment un niveau significativement plus élevé de granzyme B que les cellules T DN non-stimulées par l’allo-antigène. Ceci suggère que l’immunosuppression induite par les cellules T DN stimulées pourrait passer par la voie granzyme B. Le mécanisme utilisé par ces cellules reste à être confirmé par nos futures expériences. / Graft-versus-Host Disease (GvHD) is a major cause of morbidity and mortality in patients receiving an allogeneic Hematopoietic Stem Cell Transplantation (HSCT). Many regulatory T cell populations have been studied and shown to have immunosuppressive properties in GvHD. Among these populations, Double Negative CD4-CD8-TCRαβ+ regulatory T cells (DN T) have been described. These cells represent 1-3% of all T cell lymphocytes and are known to have antigen-specific inhibitory functions of the immune response. The suppression of an immune response by DN T cells seems to be an important mechanism involved in the induction of tolerance to allo-antigens. Moreover, these cells also confer immune tolerance in models of allogeneic and xenogenic grafts. DN T cells have the ability to suppress syngeneic T CD4+ and T CD8+ cells in an antigen-specific manner. Therefore, these DN T cells can inhibit the reaction caused by syngeneic effector cells against a specific alloantigen to which they have been previously exposed. However, they cannot inhibit a reaction directed against an unknown alloantigen. Human DN T cells have been isolated and characterized as cells that have the capacity to interact with APCs by cell-to-cell contact, just like in mice. However, their immunomodulatory properties are still unknown in humans. The goal of our project was to study the role and immunomodulatory functions of human DN T cells in Mixed Lymphocyte Reactions (MLR). The MLRs have allowed us to demonstrate that DN T cells, after having been stimulated by an allo-antigen, have an antigen-specific inhibitory function towards the syngeneic effector cells reacting against the same alloantigen that they have been exposed to. Interestingly, they do not inhibit the reaction of these effector cells against an unknown alloantigen. However, stimulated DN T cells did not modulate the expression of the activation markers expressed by the effector cells and did not give a death signal to these cells either. Moreover, we also wanted to study how DN T cells have an immunosuppressive activity. Therefore, we compared the expression of Granzyme B in stimulated versus non-stimulated cells. Our results suggest that DN T cells may use the Granzyme B pathway to immunosuppress the effector cells. In conclusion, our results demonstrate that DN T cells have an antigen-specific inhibitory function. The mechanism used by these DN T cells remains to be confirmed with our future experiments.

En stoisk livsstilstrend fick svensk mediauppmärksamhet år 2019, vilket har medfört flera nya hälso - och livsstilsgurus, flera påståenden hur man bör leva sitt liv, vilka beteenden som är avsevärda, vad som anses hälsosamt, och ansatser om vilka typer av personer anses vara experter om livsstilen. Syftet med denna uppsats har varit att utifrån ett språkvetenskapligt perspektiv, undersöka hur stoicism och de personer som anses vara stoiker konstrueras språkligt i tidningen DN (Dagens Nyheter). I konstruktionerna har undersökts närmare vilka värden och värderings framställs, samt vilka ojämna maktförhållanden och diskrimineringar potentiellt formas mot sociala grupper. Materialet som analyserats är två artiklar vilka delar den stoiska trenden som huvudämne, för att kunna göra enkompletterande analys av hur ämnet omskrivs. Metodologiskt har först gjorts en appraisal-analys för attidentifiera olika värden och värderingar som förekommer på textuell nivå, som sedan kombineras meden kritisk diskursanalys utifrån Faircloughs tredimensionella modell för att avtäcka eventuella makt asymmetrier. I texterna kunde särskilt en hälso- och livsstilsdiskurs identifieras. Den stoiska trenden konstrueras som en livsstil vilket upphöjs med hjälp av framförallt intensifierande adjektiv till ett ideal, tillsammans med dess företrädare vilka positioneras som gurus och hälsoexperter. Det framkom en homogen representation av dessa experter som män, där kvinnor utesluts. Resultatet visar även på att att hälso- och livsstilsdiskurserna kunde var moraliserande, med potential att utesluta individer från socialt utsatta grupper.

[pt] A determinação do limiar de propagação de trincas de fadiga oferece uma gama de utilização dos materiais na engenharia, na aplicação a projetos que sejam tolerantes ao dano. [delta]Kth é uma ferramenta que permite o estabelecimento de um critério de carregamento sob o qual as trincas não crescerão significativamente durante as operações de serviço do componente. Para sua determinação ser amplamente aplicada, foi necessário estabelecer e estudar os fatores que pudessem influenciá-lo positiva ou negativamente. Dentre os quais, a microestrutura apresentou grande importância porque precisou ser controlada desde a fabricação do material até a colocação em serviço do componente acabado. A finalidade do componente e os esforços a que são submetidos quando em serviço determinam a seleção do material. Assim, enumerou-se características básicas e requisitos mínimos que atenderam a segurança e reduziram a relação custo-benefício. Foram selecionados materiais (SAE 4340 e SAE 4140) cuja composição química e propriedades mecânicas fossem semelhantes ao 42CrMo4 para que pudessem servir de alternativa no emprego de eixos-virabrequim a fim de propor uma solução no problema prático o qual estes vinham rompendo muito abaixo do limite de vida em fadiga a que se propunham. Os resultados obtidos se mostraram satisfatórios pois nos ensaios de propagação de trinca de um dos materiais escolhidos (SAE 4140) obteve-se [delta]Kth de 12,48 MPa[raiz quadrada]m e no material de referência, [delta]Kth (médio) de 6,1 MPa[raiz quadrada]m. A observação da microestrutura do material de referência pode, com ressalvas, ser visto como indicativo de corroboração do resultado apresentado no [delta]Kth, pois foram localizadas ocorrências de inclusões dispersas na matriz metálica. / [en] The determination of the fatigue crack propagation threshold offers a range of materials utilization in engineering, in application to projects that are tolerant to damage. [delta]Kth is a tool that allows the establishment of a loading criterion under which the cracks will not grow significantly during the service operations of the component. For its determination to be widely applied, it was necessary to establish and study the factors that could influence it positively or negatively. Among them, the microstructure was of great importance because it had to be controlled from the material manufacturing to the placing putting into service of a finished component. The aim of the component and the stresses to which it is subjected when in service determine the material selection. Thus, basic characteristics and minimum requirements were listed which have attend the safety and has reduced cost-benefit ratio. Materials (SAE 4340 and SAE 4140) were selected whose chemical composition and mechanical properties that were similar to DIN 42CrMo4 so that they could serve as an alternative in the use of crankshafts to propose a solution in the practical problem which they were breaking well below the limit fatigue life that they proposed. The results obtained were satisfactory because in the fracture toughness tests of one of the chosen materials (SAE 4140) [delta]Kth of 12.48 MPa[square root]m was obtained and in the reference material, [delta]Kth(average) of 6.1 MPa[square root]m. The observation of the microstructure of the reference material may indicate, with restrictions, as a corroboration of the result presented in the [delta]Kth, because occurrences of dispersed inclusions in the steel matrix.

<p>Abstract</p><p><strong>Titel: </strong>Diagnosing accountability, a study of accountability of UNT and DN’s medical journalism  ( Diagnos av ansvarstagande, en studie av accountability inom DN och UNT:s medicinjournalistik)</p><p><strong>Number of pages: </strong>45 (59 including enclosures)</p><p><strong>Author:</strong> Alexandra Johansson</p><p><strong>Tutor: </strong>Göran Svensson</p><p><strong>Course: </strong>Media and Communication studies C<strong> </strong></p><p><strong>Period: </strong>Fall 2008<strong> </strong></p><p><strong>University: </strong>Division of Media and Communication, Department of Information Science, Uppsala University</p><p><strong>Purpose/Aim: </strong>To evaluate the quality of medical journalism in DN and UNT according to the theories of accountability and responsibility in the media.</p><p><strong>Material/Methods: </strong>The materials and methods used in this thesis are 14 articles from DN and 14 articles from UNT that have been analyzed according to the criterion set forth by Media Doctors, an Australian media accountability system.</p><p><strong>Main Results: </strong>The results showed that DN scored an average of 57% “satisfactory” and UNT scored an average of 52% “satisfactory” of Media Doctors rating scale. Strengths were an educational tone and an insightful review of the newest findings in the scientific community. Weaknesses were poor transparency and a tendency to neglect full disclosure of the scientific findings and sources. Focus was also show to be on research and not on information for potential patients. Media Doctor was also shown to be a well formatted media accountability system which gave insights to the broad uses of such systems.</p><p><strong>Keywords: </strong>Medical journalism, DN, UNT, Accountability, media accountability systems (MAS), Media Doctor.</p><p> </p><p> </p>

<p>ABSTRACT</p><p>Purpose/Aim: This essay aims to investigate what picture (obvious and hidden) of men and womens equality in our society that is to be found on DN Debatt.</p><p>Material/Method: The material consists of 175 articles from DN Debatt during 2004. A content analysis and a rethoric analysis has then been used as a method of analysing the material.</p><p>Main results: Men are much more represented on DN Debatt then women. The underlying picture of men and women that is presented sees men as being the ones who are in charge of all the important and “big” decisions and questions in our society. Women on the other hand are pictured as, although they have reached higher positions in society, the ones who to a high degree focus on closer and more socially important issues. The investigation has found much support of Yvonne Hirdmans theories of the gender system.</p><p>Keywords: Dagens Nyheter, DN Debatt, Gender, Gender Images, Media</p>

<p>I den här uppsatsen undersöks debattartiklar under åren 1997 till 2007 på Dagens Nyheters debattsidor. Intresset ligger vid att undersöka kvalitetsrelaterade skolfrågor och särskilt hur</p><p>lärarprofessionen deltar i debatten. Som inspiration för behandling av materialet används Pierre Bourdieus ramverk med dess kapital- och fältbegrepp. Resultaten av undersökningen indikerar att lärarna har stort utrymme i debatten. Dessutom hörs statliga politiker i hög grad.</p><p>Skolverket, akademiker och näringsliv deltar i betydligt mindre omfattning, men befinner sig inte på något sätt på marginalen. Kommunen däremot lyser med sin nästintill absoluta frånvaro. Den övergripande riktningen i debatten är att kvalitet nås genom en stärkt</p><p>lärarprofession. De övergripande teman som finns i debatten utgår ifrån lärarprofessionens centrala betydelse.</p>

Abstract Titel: Diagnosing accountability, a study of accountability of UNT and DN’s medical journalism  ( Diagnos av ansvarstagande, en studie av accountability inom DN och UNT:s medicinjournalistik) Number of pages: 45 (59 including enclosures) Author: Alexandra Johansson Tutor: Göran Svensson Course: Media and Communication studies C Period: Fall 2008 University: Division of Media and Communication, Department of Information Science, Uppsala University Purpose/Aim: To evaluate the quality of medical journalism in DN and UNT according to the theories of accountability and responsibility in the media. Material/Methods: The materials and methods used in this thesis are 14 articles from DN and 14 articles from UNT that have been analyzed according to the criterion set forth by Media Doctors, an Australian media accountability system. Main Results: The results showed that DN scored an average of 57% “satisfactory” and UNT scored an average of 52% “satisfactory” of Media Doctors rating scale. Strengths were an educational tone and an insightful review of the newest findings in the scientific community. Weaknesses were poor transparency and a tendency to neglect full disclosure of the scientific findings and sources. Focus was also show to be on research and not on information for potential patients. Media Doctor was also shown to be a well formatted media accountability system which gave insights to the broad uses of such systems. Keywords: Medical journalism, DN, UNT, Accountability, media accountability systems (MAS), Media Doctor.