Polycyclic aromatic hydrocarbons, produced by anthropological and natural activities, are hazardous through formation of oxidative radicals and DNA adducts. Growth of Mycobacterium sp. strain KMS, isolated from a contaminated soil, on the model hydrocarbon pyrene induced specific proteins. My work extends the study of isolate KMS to the gene level to understand the pathways and regulation of pyrene utilization. Genes encoding pyrene-induced proteins were clustered on a 72 kb section on the KMS chromosome but some also were duplicated on plasmids. Skewed GC content and presence of integrase and transposase genes suggested horizontal transfer of pyrene-degrading gene islands that also were found with high conservation in five other pyrene-degrading Mycobacterium isolates. Transcript analysis found both plasmid and chromosomal genes were induced by pyrene. These processes may enhance the survival of KMS in hydrocarbon-contaminated soils when other carbon sources are limited. KMS also grew on benzoate, confirming the functionality of an operon containing genes distinct from those in other benzoate-degrading bacteria. Growth on benzoate but not on pyrene induced a gene, benA, encoding a benzoate dioxygenase α-subunit, but not the pyrene-induced nidA encoding a pyrene dioxygenase α-subunit; the differential induction correlated with differences in promoter sequences. Diauxic growth occurred when pyrene cultures were amended with benzoate or acetate, succinate, or fructose, and paralleled delayed expression of nidA. Single phase growth and normal expression of benA was observed for benzoate single and mixed cultures. The nidA promoters had potential cAMP-CRP binding sites, suggesting that cAMP could be involved in carbon repression of pyrene metabolism. Growth on benzoate and pyrene requires gluconeogenesis. Intermediary metabolism in isolate KMS involves expression from genes encoding a novel malate:quinone oxidoreductase and glyoxylate shunt enzymes. Generation of C3 structures involves transcription of genes encoding malic enzyme, phosphoenolpyruvate carboxykinase, and phosphoenolpyruvate synthase. Carbon source modified the transcription patterns for these genes. My findings are the first to show duplication of pyrene-degrading genes on the chromosome and plasmids in Mycobacterium isolates and expression from a unique benzoate-degrading operon. I clarified the routes for intermediary metabolism leading to gluconeogenesis and established a potential role for cAMP-mediated catabolite repression of pyrene utilization.

Lucy Snowe, the heroine of <i>Villette</i>, Charlotte Brontës final novel, is in constant conflict with the dichotomies of patriarchal culture. As she is perpetually torn between the opposing forces of patriarchy, Lucy Snowe inhabits what she calls her own <i>still shadow-world</i> (Brontë164). This thesis explains the nature of the intermediate space Lucy Snowe occupies and examines its repercussions on her mental state. Chapter One theorizes the effect of patriarchal dichotomies on Lucy Snowe to demonstrate that her mental conflict has its roots in the female experience of the opposition between nature and culture. Chapter Twos analysis of the nineteenth-century medical understanding of madness shows that Lucy Snowes melancholy is a symptom of the intermediacy created by conflicting patriarchal expectations. Chapter Three compares Lucy Snowe to the female figure in patriarchal master narratives, which draws attention to the serious consequences of patriarchal culture on women and demonstrates that Lucy is representative of women in conflict with patriarchal expectations. Ultimately, as part of Charlotte Brontës endeavor to represent truth rather than reality, Villette challenges patriarchal expectations of women and presents a different vision of womanhood.

In comparison to other Pseudomonas, P. aeruginosa grows poorly in L-lysine as a sole source of nutrient while fast growth mutants can be obtained. The proposed catabolic pathway involves lysine decarboxylation to cadaverine and its subsequent degradation through g-glutamylation pathway to d-aminovalerate and glutarate. The lysine decarboxylase A (ldcA) gene, previously identified as a member of the ArgR regulon of L-arginine metabolism, was found essential for L-lysine catabolism. The ldcA gene encodes a decarboxylase which takes L-lysine but not L-arginine as substrate. Contrarily, the ldcA expression was inducible by L-arginine but not by L-lysine. This peculiar arginine control on lysine utilization was also noted from uptake experiments. The lack of lysine-responsive control on lysine catabolism and its tight connection to arginine regulatory network provided an explanation of lysine as poor nutrient for P. aeruginosa. Catabolism of cadaverine, a product from lysine decarboxylation, was investigated and compared to that of putrescine, another diamine of similar biochemical properties that is derived from arginine and ornithine. While the g-glutamylation pathway was first reported in E. coli for putrescine utilization, an expanded version of this pathway was found in P. aeruginosa with redundant enzymes for polyamine degradation. The PauR protein was identified as a transcriptional repressor of genes for the catabolism of putrescine and cadaverine, as well as their corresponding downstream metabolites, g-aminobutyrate (GABA) and d-aminovalerate (AMV). PauR shows distinct dimer configuration after glutaraldehyde crosslinkage, and possible conformational changes could be triggered by the presence of putrescine and cadaverine, but not GABA. A newly identified ABC transport system, encoded by the agtABCD operon, was found important for the uptake of GABA and AMV; and expression of which is controlled by the AgtSR two-component system. The CbrAB two-component system was proposed to regulate the catabolite repression control protein Crc through a small RNA CrcZ. A consensus CbrB recognition sequence was proposed based on the conserved palindromic nucleotide sequence in the upstream activating sequence of the crcZ promoter. Genetic studies indicated utilization of arginine, lysine and diamines (but not histidine, GABA and AMV) might be under CbrAB regulation through the CbrAB/CrcZ/Crc system in P. aeruginosa.

Lucy Snowe, the heroine of <i>Villette</i>, Charlotte Brontës final novel, is in constant conflict with the dichotomies of patriarchal culture. As she is perpetually torn between the opposing forces of patriarchy, Lucy Snowe inhabits what she calls her own <i>still shadow-world</i> (Brontë164). This thesis explains the nature of the intermediate space Lucy Snowe occupies and examines its repercussions on her mental state. Chapter One theorizes the effect of patriarchal dichotomies on Lucy Snowe to demonstrate that her mental conflict has its roots in the female experience of the opposition between nature and culture. Chapter Twos analysis of the nineteenth-century medical understanding of madness shows that Lucy Snowes melancholy is a symptom of the intermediacy created by conflicting patriarchal expectations. Chapter Three compares Lucy Snowe to the female figure in patriarchal master narratives, which draws attention to the serious consequences of patriarchal culture on women and demonstrates that Lucy is representative of women in conflict with patriarchal expectations. Ultimately, as part of Charlotte Brontës endeavor to represent truth rather than reality, Villette challenges patriarchal expectations of women and presents a different vision of womanhood.

This is the first book-length philosophical study of Husserl’s transcendental phenomenology and Freud’s theory of the unconscious. The book investigates the possibility for Husserl’s transcendental phenomenology to clarify Freud’s concept of the unconscious with a focus on the theory of repression as its centre. Repression is the unconscious activity of pushing something away from consciousness, while making sure that it remains active as something foreign within us. How this is possible is the main problem addressed in the work. Unlike previous literature (including Ricœur, Merleau-Ponty and Derrida) this book makes full use of the resources of genetic phenomenology and passivity in the attempt to clarify the Freudian unconscious. The central argument developed is that the structure of the lebendige Gegenwart as the core of Husserl’s theory of passivity consists of preliminary forms of bodily kinaesthesia, feelings and drives in a constantly ongoing process where repression occurs as a necessary part of all constitution. The clarification of Freudian repression thus takes place by showing how it presupposes a broad conception of consciousness such as that presented by Husserl’s genetic phenomenology. By arguing that “repression” is central to any philosophical account of subjectivity, this book takes on the most distinct challenge to philosophy posed by Freud.

Metabolically versatile bacteria have evolved diverse strategies to adapt to different environmental niches and respond to fluctuating physico-chemical parameters. In order to survive in soil and water habitats, they employ specific and global regulatory circuits to integrate external and internal signals to counteract stress and optimise their energy status. One strategic endurance mechanism is the ability to choose the most energetically favourable carbon source amongst a number on offer. Pseudomonas putida strains possess large genomes that underlie much of their ability to use diverse carbon sources as growth substrates. Their metabolic potential is frequently expanded by possession of catabolic plasmids to include the ability to grow at the expense of seemingly obnoxious carbon sources such as phenols. However, this ability comes with a metabolic price tag. Carbon source repression is one of the main regulatory networks employed to subvert use of these expensive pathways in favour of alternative sources that provide a higher metabolic gain. This thesis identifies some of the key regulatory elements and factors used by P. putida to supress expression of plasmid-encoded enzymes for degradation of phenols until they are beneficial. I first present evidence for a newly identified DNA and RNA motif within the regulatory region of the gene encoding the master regulator of phenol catabolism – DmpR. The former of these motifs functions to decrease the number of transcripts originating from the dmpR promoter, while the latter mediates a regulatory checkpoint for translational repression by Crc – the carbon repression control protein of P. putida. The ability of Crc to form repressive riboprotein complexes with RNA is shown to be dependent on the RNA chaperone protein Hfq – a co-partnership demonstrated to be required for many previously identified Crc-targets implicated in hierarchical assimilation of different carbon sources in P. putida. Finally, I present evidence for a model in which Crc and Hfq co-target multiple RNA motifs to bring about a two-tiered regulation to subvert catabolism of phenols in the face of preferred substrates – one at the level of the regulator DmpR and another at the level of translation of the catabolic enzymes.

Post-Soviet Azerbaijan is often portrayed as a very secular country. Thus the mobilization of mosque communities in the late 1990s and their conflictual relationship with the authorities came as a surprise. The main aim of the dissertation is to shed light on this mobilization, focusing on the Sunni Abu Bakr and the Shi’ite Juma mosque communities in Baku. On the premise that Islamic mobilization may be interpreted as a “social movement”, internal, contextual and interactional aspects of mobilization have been studied. The analysis is chiefly based on interviews conducted in Baku in 2004/2005 with Imams, worshippers, religious and secular authorities. The study finds that young people looking for new approaches to religion have been drawn to these communities, where they encounter an independent, educated, conscientious clergy and, indeed, a “new” religion. This “sovereign” Islam does not go down well with authorities who fear politicization of religion. The Soviet heritage has provided them with a view of religion as something that should not be publicly displayed and with the institutions to control religion. Another key feature whose impact on state policy towards religious organizations cannot be underestimated is the fear of imported radicalism. A look at Islamic mobilization in North Caucasus, Tajikistan and Uzbekistan reveals many similarities, yet one momentous difference is the harsher repression in these contexts, which decreases the chances of a non-radical mobilization. The thesis concludes that the role of the state in mobilization processes in non-democratic contexts is crucial but counterintuitive, as the regimes’ efforts to stop the mobilization of movements actually leads to its intensification. In Azerbaijan, official pressure brings community members closer together and strengthens their resolve, rather than putting an end to mobilization. It also puts a spotlight on these communities which lights up the way for others in search of something new.

Esta tesis doctoral pretende arrojar luz sobre el mayor debate intelectual mantenido con respecto al Ser de España bajo el franquismo (1936-1975). La querella ocupó no pocas páginas de autores tan importantes para el siglo XX español como fueron, el falangista y médico Pedro Laín Entralgo (1908-2001), y el monárquico e historiador Rafael Calvo Serer (1916-1988).La publicación, en 1949, de sendos volúmenes, España como problema, por parte de Laín Entralgo, y la respuesta, España, sin problema, de la mano de Calvo Serer, estimuló una discusión que venía de lejos en el panorama intelectual español, el conocido como Problema de España.Como se intentará demostrar, los contenidos de la discusión podían parecer inocuos a ojos de muchos españoles, pero lo que realmente se estaba discutiendo en las páginas de los mencionados autores era una configuración y eventual salida del Régimen creado por el Generalísimo Francisco Franco (1892-1975). / This thesis aims at shed light on the most relevant debate held on the Being of Spain under Francoism (1936-1975). This dispute occupied many pages in the works of two of the most important authors in the 20th century Spain. On the one hand, the Falangist and doctor Pedro Laín Entralgo (1908-2001), on the other, the Monarchist and historian Rafael Calvo Serer (1916-1988). With two books published in 1949, España como problema, by Laín Entralgo, and the answer, España, sin problema, by Calvo Serer, promoted a discusión that was already present in the Spanish intellectual environment, the Spanish Problem.As we want to demonstrate, the contents of these two books seemed innocuous to many Spaniards, but what they were really discussing in these pages was the configuration and eventual way out to the political regime created by Generalissimo Francisco Franco (1892-1975).

Carbon catabolite repression in A. nidulans is a regulatory system which allows the organism to utilize the most preferable carbon source by repressing the expression of genes encoding enzymes utilizing alternative carbon sources. A ubiquitination pathway was shown to be one of the key mechanisms which regulate carbon source utilization, when creB was found to encode a deubiquitinating enzyme. Strains containing mutations in creB show loss of repression for some metabolic pathways in carbon catabolite repressing conditions, and also grow very poorly on several sole carbon sources such as quinate and proline, suggesting CreB plays multiple roles in the cell. This work describes the analysis of the interaction of CreB with CreA, and with PrnB and QutD. Various epitope-tagged versions of CreA were expressed in A. nidulans, and an internally located HA-epitope tag was found to allow detection of CreA using Western analysis. A diploid strain was constructed between strains containing HA-tagged CreA and FLAG-tagged CreB. When CreB was immunoprecipitated, HA-tagged CreA was also precipitated in the diploid, indicating that CreA and CreB are present in a complex in vivo. To determine whether CreA is a ubiquitinated protein, a version of CreA that was tagged with both an HA epitope and a His-tag was expressed in A. nidulans, and protein extracts were precipitated with an UbiQapture™-Q matrix. Western analysis was used to show that CreA was present in the precipitate. These findings suggest that CreA is a ubiquitinated protein, and a target of the CreB deubiquitination enzyme. To determine whether the proline permease (PrnB) is a direct substrate of CreB, plasmids to express epitope-tagged versions of PrnB were constructed and introduced into the prnB mutant strain. No tagged protein could be detected by Western analysis, even when these constructs were over-expressed from the gpdA promoter. However, a construct to express an HA epitope tagged version of quinate permease (QutD) fully complemented the qutD mutant strain, and HA-tagged QutD could be easily detected in Western analysis when probed with the anti-HA monoclonal antibody. A diploid strain was made between a complementing transformant and a strain expressing a FLAG-tagged CreB construct. When QutDHA was immunoprecipitated, CreBFLAG was detected in the immunoprecipitate of the diploid. A proportion of QutDHA was also co-precipitated in the diploid when CreBFLAG was immunoprecipitated. Thus, CreB is present in a complex with QutD in vivo. Further results showed that the concentration of QutD in the cell is lower in a creB null mutant background than in the wild-type background, indicating that deubiquitination is required to prevent protein turnover. Northern analysis of mRNA showed that the failure of creB mutant strains to grow on quinate medium was not due to a failure of transcriptional induction of qutD, as the amount of mRNA was not lower in a creB1937 mutant background compared to the wild-type. Furthermore, experiments were undertaken that showed that QutD is a ubiquitinated protein. These findings suggest that quinate permease is regulated through deubiquitination involving the CreB deubiquitination protein in A. nidulans. In addition to the candidate protein approach asking whether CreA is a substrate of CreB, a proteomics approach was also used to identify proteins that interact with CreA. However, no clear interacting proteins were identified using this approach. / Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2008

Si la transgression se rencontre dans l’historiographie, elle n’a cependant jamais donné lieu à une véritable théorisation. C’est l’objet de cette thèse d’en dresser une définition qui en fait la contestation d’une loi en appui sur une volonté, la conscience d’une possible sanction, la projection d’un au-delà normatif et une capacité de nuisance contre l’autorité. La validité du concept a été éprouvée en le confrontant aux rapports de police quotidiens rédigés dans le Nancy occupé de 1940 à 1944. L’objectif est d’étudier comment la transgression se transforme en un phénomène d’envergure au point d’apparaitre comme une nouvelle normalité. Dans un premier temps, l’analyse des conditions de la déviance dresse l’état des lieux d’une société provinciale en temps de guerre et les mutations physiques et humaines du territoire urbain. Les champs de la transgression dévoilent, ensuite, le caractère protéiforme de la désobéissance au niveau politique, des opinions, de la violence, de l’économie, des mœurs, des mobilités, des identités et des attitudes. On y croise à la fois les phénomènes de collaboration, de Résistance, de déportation, les comportements journaliers de survie aussi bien que les imaginaires et les représentations. Enfin, l’analyse approche le transgresseur à hauteur d’homme pour dresser une sociologie du crime, construire le portrait-type du déviant et approcher des figures singulières de résistants, de Justes, ou de conformiste critique. Au final, les 1550 jours d’occupation plongent Nancy dans un hors-temps où l’« a-normal » est la règle. L’angle de la transgression permet de comprendre les stratégies mises en œuvre par le corps social pour tenter de s’y adapter / Although transgression has already been dealt with in historiography, its theorising has never been fully and thoroughly achieved. This thesis aimt at providing a definition that will be read as the result of a law-challenging will combined with the awareness of looming threats, the ability to build up strategy beyond a normative framework together with the ability to undermine existing power. The concept has been tested and validated through 1940-1944 daily police reports that were written in then German-occupied Nancy. The aim is to study how transgression becomes and overwhelming phenomenon so that it almost looks like conventional normality. First, the study of the way deviance was implemented gives us information about war-time provincial society and about physical changes in urban landscape. Transgression thus reveals its multifaceted aspects in political disobedience, opinions, violence, economics, morals, transport, identity and behaviour. There we’ll follow the markers of collaboration with the nazis, resistance, deportation, survival behaviour as well as psyche and mental representations. Last the transgressor will be analysed on a human scale in order to build up a sociology of crime, draw up the typical profile of deviant people and make you closer to notable figures of resistants fighters, those entitled « Righteous among the nations » and meet conventional yet critical citizens. Those 1550 days in German-occupied Nancy give the opportunity to steep yourself into times when ab-normality was the rule. The view through the prism of transgression should allow us to better understand the strategies implemented by society to fit with the situation