Durante o processo de reparação tecidual, o fator de transformação de crescimento <font face=\"symbol\">b1 (TGF-<font face=\"symbol\">b1) apresenta um importante papel na regulação da expressão da <font face=\"symbol\">a-actina de músculo liso (<font face=\"symbol\">a-AML) e portanto, na diferenciação miofibroblástica. Como os fibroblastos pulpares apresentam características peculiares, com a expressão de proteínas específicas que os diferem de fibroblastos de outros tecidos conjuntivos, o presente estudo avaliou in vitro se o TGF-<font face=\"symbol\">b1 aumenta a expressão de <font face=\"symbol\">a-AML em fibroblastos pulpares humanos comparando-os com fibroblastos de gengiva. Para tal, diferentes doses de TGF-<font face=\"symbol\">b1 (5 à 10 ng/ml) foram adicionadas às culturas de células, sendo a expressão da <font face=\"symbol\">a-AML analisada por imunofluorescência e western-blotting. Ambos os tipos celulares imunoexpressaram <font face=\"symbol\">a-AML mesmo sem o tratamento com o TGF-<font face=\"symbol\">b1, estando aumentada consideravelmente, quando o TGF-<font face=\"symbol\">b1 foi adicionado às culturas. Os resultados do presente estudo demonstraram que o TGF-<font face=\"symbol\">b1 induz a expressão de <font face=\"symbol\">a-AML, sugerindo a indução do fenótipo miofibroblástico em fibroblastos pulpares. / Transforming growth factor-beta 1 (TGF-<font face=\"symbol\">b1) has been related to induce the expression of <font face=\"symbol\">a-smooth muscle actin (<font face=\"symbol\">a-SMA) in fibroblasts during repair. Since pulpal fibroblasts seem to be somewhat different from other fibroblasts, the present study investigated in vitro whether TGF-<font face=\"symbol\">b1 enhances the expression of <font face=\"symbol\">a-SMA in human pulpal fibroblasts. TGF-<font face=\"symbol\">b1 was added in doses between 5-10 ng/ml to cultures of both dental pulp and gingiva human fibroblasts. The expression of <font face=\"symbol\">a-SMA was analyzed by immunofluorescence and western-blotting. Both cell types were immunoreactive for <font face=\"symbol\">a-SMA even without TGF-<font face=\"symbol\">b1. When TGF-<font face=\"symbol\">b1 was added to cell cultures, the expression of <font face=\"symbol\">a-SMA increased dramatically in pulpal fibroblasts, independent of the concentration used. It was confirmed by the western blot analysis. The present findings showed that TGF-<font face=\"symbol\">b1 up-regulated the expression of <font face=\"symbol\">a-SMA thus inducing pulpal fibroblasts to acquire the myofibroblast phenotype.

Homeostase do O2 é essencial para a sobrevivência e desenvolvimento fisiológico dos organismos. A falta de O2 nos tecidos é um fator subjacente comum na morbidade e mortalidade de situações clínicas como na Síndrome do Desconforto Respiratório Agudo (ARDS). Na retomada da homeostase as células mielóides exercem suas funções especializadas nas áreas de hipóxia. A adaptação destas células nesta condição depende dos produtos do gene HIF-1<font face=\"Symbol\">&#945, fator de transcrição que responde às mudanças de O2. Neste trabalho caracterizamos os mecanismos celulares e moleculares operantes no estado de hipóxia em resposta à reação inflamatória aguda no pulmão induzida pela I/R ateria mesentérica em duas linhagens de camundongos geneticamente selecionadas para máxima (AIRmax) ou mínima (AIRmin) Resposta Inflamatória Aguda (AIR). Observamos uma alta reação inflamatória aguda em resposta a I/R. Altos níveis de expressão dos genes envolvidos em situações de hipóxia Hif-1<font face=\"Symbol\">&#945, Vhl e das citocinas Il-1<font face=\"Symbol\">&#946 e Il-6 mostraram-se relacionados com a alta AIR nos camundongos AIRmax. / Oxygen homeostasis is essential for survival and physiologic development of organisms. Lack of O2 in tissues is a common underlying factor in morbidity and mortality for numerous serious medical conditions such as the Acute Respiratory Distress Syndrome (ARDS). For homeostasis recovery the myeloid cells exert their functions in specialized areas of hypoxia. The adaptation of myeloid cells in low O2 tissue depends on the HIF-1<font face=\"Symbol\">&#945 gene products. Hif-1<font face=\"Symbol\">&#945 is a transcription factor that responds to O2 levels change. In this study we characterize, in two lines of mice selected for maximal (AIRmax) or minimal (AIRmin) Acute Inflammatory response (AIR), the cellular and molecular mechanisms operating in the hypoxia state during an acute inflammatory reaction in the lung parenchyma produced by mesenteric artery Ischemia. We observed an acute inflammatory reaction with high levels of Hif-1<font face=\"Symbol\">&#945 and Vhl genes expression involved in hypoxia conditions and IL-1<font face=\"Symbol\">&#946 and IL-6 genes showed related to high AIR in AIRmax mice.

Throughout history and in today’s society, typography has been and still is without doubt a great part of communication. Behrens, an important designer from the modernist movement, believed that after architecture, typography provided “…the most characteristic picture of a period” and “…development of people”. Typography carries meaning and associations, built on the contexts and the design it is used in, that finally creates a typographic image. The Blackletter style Fraktur, and Helvetica were born to serve a purpose connected to power. Important for this research is to understand in detail, the origin of that power and its position: Blackletter portrays features of the Gothic architecture, expressing religious emotions and civic pride, intended for effective writing, and was predominant in religious and educational contexts. This improvement of writing was a necessity for the development of the society during the medieval times; for both educational and financial reasons. As Fraktur became a symbol of Germany, the today’s connotations towards oppression and Nazism were inevi­table as the Third Reich continued to use it until it was banned in 1941. Meanwhile, the post war modernism in the 20th Century, sought objectivity, simplicity and readability in their design, to erase any carried meaning or associations that could have a misleading effect on the information. This period of time paved way for a corporate culture, with approaches towards rationalist and function­alist methods, that expresses authority and reliability. Achieving brand recognition for a wide range of products and contexts was required by the graphic designers. Through workshops that document people’s associations and comments on the shapes and typographic images that both Helvetica and Fraktur create, and visual research made in forms of sketching and adding Fraktur features to signs of institutions and public sectors, I have investigated Fraktur’s tainted image of oppression and political sentiments with the help of Helvetica as the contemporary norm.

Durante o processo de reparação tecidual, o fator de transformação de crescimento <font face=\"symbol\">b1 (TGF-<font face=\"symbol\">b1) apresenta um importante papel na regulação da expressão da <font face=\"symbol\">a-actina de músculo liso (<font face=\"symbol\">a-AML) e portanto, na diferenciação miofibroblástica. Como os fibroblastos pulpares apresentam características peculiares, com a expressão de proteínas específicas que os diferem de fibroblastos de outros tecidos conjuntivos, o presente estudo avaliou in vitro se o TGF-<font face=\"symbol\">b1 aumenta a expressão de <font face=\"symbol\">a-AML em fibroblastos pulpares humanos comparando-os com fibroblastos de gengiva. Para tal, diferentes doses de TGF-<font face=\"symbol\">b1 (5 à 10 ng/ml) foram adicionadas às culturas de células, sendo a expressão da <font face=\"symbol\">a-AML analisada por imunofluorescência e western-blotting. Ambos os tipos celulares imunoexpressaram <font face=\"symbol\">a-AML mesmo sem o tratamento com o TGF-<font face=\"symbol\">b1, estando aumentada consideravelmente, quando o TGF-<font face=\"symbol\">b1 foi adicionado às culturas. Os resultados do presente estudo demonstraram que o TGF-<font face=\"symbol\">b1 induz a expressão de <font face=\"symbol\">a-AML, sugerindo a indução do fenótipo miofibroblástico em fibroblastos pulpares. / Transforming growth factor-beta 1 (TGF-<font face=\"symbol\">b1) has been related to induce the expression of <font face=\"symbol\">a-smooth muscle actin (<font face=\"symbol\">a-SMA) in fibroblasts during repair. Since pulpal fibroblasts seem to be somewhat different from other fibroblasts, the present study investigated in vitro whether TGF-<font face=\"symbol\">b1 enhances the expression of <font face=\"symbol\">a-SMA in human pulpal fibroblasts. TGF-<font face=\"symbol\">b1 was added in doses between 5-10 ng/ml to cultures of both dental pulp and gingiva human fibroblasts. The expression of <font face=\"symbol\">a-SMA was analyzed by immunofluorescence and western-blotting. Both cell types were immunoreactive for <font face=\"symbol\">a-SMA even without TGF-<font face=\"symbol\">b1. When TGF-<font face=\"symbol\">b1 was added to cell cultures, the expression of <font face=\"symbol\">a-SMA increased dramatically in pulpal fibroblasts, independent of the concentration used. It was confirmed by the western blot analysis. The present findings showed that TGF-<font face=\"symbol\">b1 up-regulated the expression of <font face=\"symbol\">a-SMA thus inducing pulpal fibroblasts to acquire the myofibroblast phenotype.

Homeostase do O2 é essencial para a sobrevivência e desenvolvimento fisiológico dos organismos. A falta de O2 nos tecidos é um fator subjacente comum na morbidade e mortalidade de situações clínicas como na Síndrome do Desconforto Respiratório Agudo (ARDS). Na retomada da homeostase as células mielóides exercem suas funções especializadas nas áreas de hipóxia. A adaptação destas células nesta condição depende dos produtos do gene HIF-1<font face=\"Symbol\">&#945, fator de transcrição que responde às mudanças de O2. Neste trabalho caracterizamos os mecanismos celulares e moleculares operantes no estado de hipóxia em resposta à reação inflamatória aguda no pulmão induzida pela I/R ateria mesentérica em duas linhagens de camundongos geneticamente selecionadas para máxima (AIRmax) ou mínima (AIRmin) Resposta Inflamatória Aguda (AIR). Observamos uma alta reação inflamatória aguda em resposta a I/R. Altos níveis de expressão dos genes envolvidos em situações de hipóxia Hif-1<font face=\"Symbol\">&#945, Vhl e das citocinas Il-1<font face=\"Symbol\">&#946 e Il-6 mostraram-se relacionados com a alta AIR nos camundongos AIRmax. / Oxygen homeostasis is essential for survival and physiologic development of organisms. Lack of O2 in tissues is a common underlying factor in morbidity and mortality for numerous serious medical conditions such as the Acute Respiratory Distress Syndrome (ARDS). For homeostasis recovery the myeloid cells exert their functions in specialized areas of hypoxia. The adaptation of myeloid cells in low O2 tissue depends on the HIF-1<font face=\"Symbol\">&#945 gene products. Hif-1<font face=\"Symbol\">&#945 is a transcription factor that responds to O2 levels change. In this study we characterize, in two lines of mice selected for maximal (AIRmax) or minimal (AIRmin) Acute Inflammatory response (AIR), the cellular and molecular mechanisms operating in the hypoxia state during an acute inflammatory reaction in the lung parenchyma produced by mesenteric artery Ischemia. We observed an acute inflammatory reaction with high levels of Hif-1<font face=\"Symbol\">&#945 and Vhl genes expression involved in hypoxia conditions and IL-1<font face=\"Symbol\">&#946 and IL-6 genes showed related to high AIR in AIRmax mice.

Os polihidroxialcanoatos (PHAs) são poliésteres acumulados por microrganismos como material de reserva. O conhecimento das vias bioquímicas e enzimas envolvidas na biossíntese e degradação dos PHAs é uma importante ferramenta para auxiliar na produção industrial. A linhagem Burkholderia cepacia IPT64 é capaz de acumular uma blenda composta de P(3HB) e P(3H4PE) a partir de sacarose. Este trabalho está focado em duas das principais enzimas envolvidas na biossíntese de PHAs: a b-cetotiolase (phaA) e a PHA sintase (phaC). A primeira está associada à especificidade pelo substrato, e a segunda é considerada a enzima chave na síntese de PHAs. Neste trabalho a linhagem mutante phaC foi avaliada quanto à atividade enzimática de PHB sintase, que se constatou ter sido perdida. A presença de mais de uma tiolase no genoma de B. cepacia foi detectada. A inativação do gene phaABc identificado anteriormente, bloqueou totalmente a síntese de P(3HB), e não promoveu o aumento da quantidade total de polímero. Este resultado indica que a tiolase identificada é responsável direta do acúmulo de P(3HB). Outra indicação é que não há uma competição das vias de síntese dos dois polímeros P(3HB) e P(3H4PE), já que não houve alteração na quantidade de P(3H4PE) acumulado, mesmo quando P(3HB) deixou de ser acumulado. / The polyhydroxyalkanoates (PHAs) are polyesters accumulated by microorganisms as storage compounds. Knowing the biochemistry pathway and enzymes involved in the biosynthesis and degradation of PHAs is an important tool to help industrial production. The Burkholderia cepacia IPT64 strain is able to accumulate a blend of P(3HB) and P(3H4PE) from sucrose. The focus of this work is on the two main enzymes involved in PHA biosynthesis: the b-ketothiolase (phaA) and the PHA synthase (phaC). The first one is associated with substrate specificity, and the second one is considered the key enzyme in PHA synthesis. In this work a mutant strain phaC was evaluated on its PHB synthase enzymatic activity, that was discovered to have been lost. The presence of other thiolases in the B. cepacia genome was detected. The inactivation of phaABc gene identified previously, blocked totally the P(3HB) synthesis, and didnt increase the polymer content. This result indicates that the identified thiolase is directly responsible for P(3HB) accumulation. Another indication is that the synthesis pathways of the two polymers, P(3HB) and P(3H4PE), dont compete with each other, because the content of P(3H4PE) was not altered, even when the P(3HB) was not accumulated.

Grafiska profiler har ofta liknande innehåll men ser självklart olika ut för olika verksamheter och varumärken. En cityförening är ett samarbete mellan flera olika aktörer som vill kommunicera med sina målgrupper och samtidigt förmedla det som föreningen tillsammans står för.  Studiens syfte är att ta reda på vad som bör ingå i en grafisk profil för en cityförening och vilka speciella faktorer som finns vid framtagandet av cityföreningars grafiska profiler. För att påvisa möjligheterna till applicering i det som undersökts, används Mjölby City som exempelfall och studien ska generera riktlinjer för Mjölby Citys grafiska profil. För att svara på studiens frågeställningar har en kvalitativ metod används i form av kvalitativa intervjuer och en fokusgrupp i form av en workshop. Intervjuerna har legat till stor vikt vid undersökningen av de speciella faktorerna, då intervjuer har gjorts med företagsrepresentanter som har skapat eller bearbetat grafiska profiler för cityföreningar. Fokusgruppen utfördes för att ta reda på Mjölby Citys identitet, något som bör ligga till grund då en grafisk profil utarbetas. En kvalitativ intervju med en kommunikationsbyrå har också gjorts för att understryka betydelsen av fokusgruppen. I analysen framkommer det att den teoretiska utgångspunkten och den framtagna empirin är eniga i flera aspekter. Studiens slutsatser bygger på att det finns en del speciella faktorer att ta hänsyn till när det gäller cityföreningars grafiska profiler. En cityförening behöver en tydlig grafisk profil och de bör ta hänsyn till att det finns en tredelad målgrupp: besökare, investerare och invånare. Det är viktigt att ta hänsyn till omgivande faktorer så som politik i området, den lokala utvecklingen och marknaden. En grafisk profil för en cityförening bör bygga på kärnvärden och en karaktäristisk identitet. Den grafiska profilen bör vara bred så att alla föreningens verksamheter inryms under den och den ska gå att kombinera med samarbetspartners visuella uttryck. Kulörer bör definieras i den grafiska profilen som kan delas in efter årstider, högtider och evenemang.  Innehållet i Mjölby Citys grafiska profil bör genomsyras av föreningens identitet och kärnvärden. Den bör innehålla en tydlig logotyp och olika harmoniserande färgpaletter för olika händelser. Den bör innehålla definierade teckensnitt, en antikva och en linjär och om Mjölby City finner en lämplig tagline bör även den finnas med i den grafiska profilen. / Corporate visual identities often a have similar kind of content, but of course they differ depending on the corporation or brand. A city center association is a collaboration of a number of different companies willing to communicate with their target groups and in the same time conveying what the association stands for. This thesis work aims to find out what should be included in a corporate visual identity of a city association and what special factors can be found in the development of these kind of corporate visual identities. To demonstrate the applicability of the study, Mjölby City is used as a case study and guidelines for Mjölby City’s visual identity will be generated. To answer the frame of questions of the study, a qualitative method is used in the form of qualitative interviews and a focus group. Considering the aim of finding special factors, the interviews have been of great importance. They were conducted with company representatives who have worked with visual identities for city associations. The focus group was conducted to find out what Mjölby City’s identity is, which is of great importance when a corporate visual identity is developed. A qualitative interview with a communications agency has also been held to emphasize the importance of the focus group. The analysis shows that the theoretical basis and the derived empirical data are in agreement in several aspects. The study findings are based on the existence of special factors to consider during the development of city association’s visual identities. A city association needs a clear visual identity and one should take into consideration that there are three target groups: visitors, investors and residents. It is important to take environmental factors into account such as policies in the region, local development and the marketplace. A city association’s visual identity should be based on core values and distinctiveness. The visual identity should be broad so that all the association’s activities are housed under it. The width is also important for the possibilities to combine it with partners’ visual identities. Colours should be defined in the visual identity program and they should be be classified according to seasons, celebrations and events. The contents of Mjölby City’s visual identity should be permeated of the association’s identity and core values. It should contain a distinct logo and various harmonizing colour palettes for different events. It should also contain defined fonts, one roman and one linear font, and if Mjölby City finds a suitable tagline this should also be included in the visual identity program.

L'apport de cette thèse est, avant tout, d'avoir montré que la structure en nappes de charriages connue plus au nord se poursuit dans ce domaine méridional de la zone briançonnaise, ce que les publications antérieures n'avaient en aucune manière permis de croire. L'ouvrage comporte une introduction qui délimite le sujet, donne l'historique des recherches antérieures et fournit un aperçu simplifié du schéma structural auquel ont conduit les recherches de l'auteur. Le corps principal est constitué par la description des données recueillies par l'auteur sous les deux angles de la stratigraphie et de la tectonique. Stratigraphie : La colonne stratigraphique est décrite, depuis le Houiller jusqu'au Nummulitique. Une attention toute particulière est portée au Jurassique, au sujet duquel sont détaillées ses variations de l'une à l'autre des différentes unités structurales distinguées. Tectonique : La description est menée selon un plan géographique en partant de la coupe fondamentale donnée par la vallée de la Haute Ubaye. Elle porte d'abord sur les montagnes des deux rives de la vallée de l'Ubaye puis sur celles du secteur mi-français, mi-italien, compris entre les vallées de l'Ubayette et de la Haute Maira. Elle met en évidence l'existence de sept nappes de charriage, imbriquées à la fois d'ouest en est et du sud vers le nord, qui sont reployées par des anticlinaux affectant le soubassement primaire de la zone et recoupées par des failles plus ou moins tardives, longitudinales par rapport à cette dernière. La distinction est faite entre des "nappes d'écaillage", avec participation du matériel siliceux permo-triasique, et des "nappes de décollement", n'intéressant que les niveaux plus récents de la colonne stratigraphique. Enfin la notion de "rétrocharrriage" correspondant à des mouvements tardifs des nappes en direction de l'Italie, est introduite et illustrée. La partie consacrée aux conclusions générales donne, en particulier, un résumé de l'histoire géologique de la région étudiée et envisage les rapports entre structure tectonique et séismicité. Des suggestions de trajets d'excursion y sont en outre fournies, en indiquant les pages où trouver les descriptions correspondantes.

Criteria for identifying prehistoric water-temples among other archaeological remains in Sardinia have not been explicitly discussed so far, making it difficult to investigate this remarkable body of evidence as a whole. This study therefore aims at elaborating a method for identifying water-temples among other fonts and wells in Sardinia, and applying it to produce a preliminary inventory. A theoretical discussion of definable criteria for assessing possible cult status in this context precedes an evaluation of the investigable wells and fonts reported in the island. Buildings found to lack cult correlates are noted in an Appendix, while buildings presenting them are inventoried in a Catalogue. A discussion of the results considering spatial, temporal and cultural aspects follows, leading to a new perspective regarding the genesis of the Sardinian water-temples. / Criteri per l'identificazione di templi ad acqua preistorici fra altri residui archeologici in Sardegna non sono stati esplicitamente discussi fin'ora, rendendo difficile l'indagine di questo straordinario insieme di evidenze come tale. Questo studio vuole perciò elaborare un metodo per identificare templi ad acqua fra altri pozzi e fonti in Sardegna, e applicarlo per produrre un inventario preliminare. Un discorso teorico di criteri definibili per valutare l'eventuale status cultuale nel contesto precede una valutazione degli indagabili pozzi e fonti segnalati nell'isola. Edifici trovati mancanti di correlati di culto sono notati in un Appendice, mentre edifici che li presentano sono inventoriati in un Catalogo. Segue una discussione dei risultati, considerando aspetti spaziali, temporali e culturali, portando ad una nuova proposa in riguardo alla genesi dei templi ad acqua sardi. / Kriterier för att identifiera förhistoriska vattentempel bland andra arkeologiska lämningar på Sardinien har hittills inte diskuterats uttryckligen, vilket gjort det svårt att undersöka denna anmärkningsvärda fyndkategori som helhet. Denna studie syftar därför till att utarbeta en metod för att identifiera vattentempel bland andra funter och brunnar på Sardinien, och tillämpa den för att göra en preliminär inventering. En teoretisk diskussion av definierbara kriterier för att bedöma eventuell kultstatus i sammanhanget föregår en utvärdering av de studerbara brunnar och källor som har rapporterats på ön. Byggnader som befunnits sakna kultkorrelat noteras i ett Appendix, medan byggnader som uppvisar dem inventeras i en Katalog. En diskussion av resultaten med rumsliga, tidsliga och kulturella aspekter i åtanke följer, och leder till ett nytt perspektiv gällande te sardiska vattentemplens tillkomst.

The Compact Linear Collider (CLIC) is a proposal for a future linear electron--positron collider that could achieve collision energies of up to 3 TeV. In the CLIC concept the main high energy beam is accelerated using RF power extracted from a high intensity drive beam, achieving an accelerating gradient of 100 MV/m. This scheme places strict tolerances on the drive beam phase stability, which must be better than 0.2 degrees at 12 GHz. To achieve the required phase stability CLIC proposes a high bandwidth (&GT;17.5 MHz), low latency drive beam "phase feedforward" (PFF) system. In this system electromagnetic kickers, powered by 500 kW amplifiers, are installed in a chicane and used to correct the phase by deflecting the beam on to longer or shorter trajectories. A prototype PFF system has been installed at the CLIC Test Facility, CTF3; the design, operation and commissioning of which is the focus of this work. Two kickers have been installed in the pre-existing chicane in the TL2 transfer line at CTF3 for the prototype. New optics have been created for the line to take these changes in to account, incorporating new constraints to obtain the desired phase shifting behaviour. Three new phase monitors have also been installed, one for the PFF input and two to verify the system performance. The resolution of these monitors must be significantly better than 0.2 degrees to achieve CLIC-level phase stability. A point by point resolution as low as 0.13 degrees has been achieved after a series of measurements and improvements to the phase monitor electronics. The performance of the PFF system depends on the correlation between the beam phase as measured at the input to the PFF system, and the downstream phase, measured after the correction chicane. Preliminary measurements found only 40&percnt; correlation. The source of the low correlation was determined to be energy dependent phase jitter, which has been mitigated after extensive efforts to measure, model and adjust the machine optics. A final correlation of 93% was achieved, improving the theoretical reduction in jitter using the PFF system from a factor 1.1 to a factor 2.7. The performance and commissioning of the kicker amplifiers and PFF controller are also discussed. Beam based measurements are used to determine the optimal correction timing. With a maximum output of around 650 V the amplifiers provide a correction range of &plusmn;5.5 &plusmn; 0.3 degrees. Finally, results from operation of the complete system are presented. A mean phase jitter of 0.28 &plusmn; 0.02 degrees is achieved, in agreement with the theoretical prediction of 0.27 &plusmn; 0.02 degrees for an optimal system with the given beam conditions. The current limitations of the PFF system, and possible future improvements to the setup, are also discussed.