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Este trabalho teve por objetivo selecionar estirpes fracas do Passion fruit woodiness virus (PWV) e avaliar o seu efeito protetor para o controle do endurecimento dos frutos do maracujazeiro. Foram selecionadas seis estirpes fracas do PWV. Três a partir de plantas de elite, encontradas em pomares severamente afetados pelo vírus (F-101, F-102 e F-103) e três a partir de bolhas formadas em folhas de maracujazeiro com mosaico (F-99, F-144 e F-145). O efeito protetor das estirpes fracas foi avaliado em maracujazeiros, em casa de vegetação e em campo. Em casa de vegetação foi observada uma proteção parcial das estirpes F-101, F-102 e F-144, contra a estirpe severa PWV-SP. Em campo, num primeiro experimento, as seis estirpes fracas selecionadas foram avaliadas e aproximadamente 4 meses após o desafio com a estirpe PWV-SP, todas as plantas apresentaram sintomas severos da doença. Diante da proteção parcial em casa de vegetação e da ausência total de proteção no experimento de campo, duas hipóteses foram apresentadas para explicar a intensificação de sintomas em maracujazeiros premunizados e desafiados com a estirpe severa do virus: a) a ocorrência de baixa concentração e/ou distribuição irregular das estirpes fracas nos tecidos das plantas premunizadas permite a infecção e estabelecimento da estirpe severa posteriormente inoculada e b) as estirpes fracas selecionadas são de uma espécie diferente de Potyvirus, serologicamente relacionada com o PWV, mas que não oferecem proteção contra a estirpe severa deste último. A primeira hipótese foi estudada repetindo-se o experimento com maracujazeiros premunizados com as estirpes F-101 e F-144, separadamente, e cultivados em campo sob condições de telado. Antes do desafio, foram feitos estudos quantitativos das estirpes F-101 e F-144, em diferentes folhas das plantas, através do DAS-ELISA indireto. Foi observada uma grande variação na concentração das estirpes fracas nos tecidos de diferentes folhas da mesma planta. Em 68,3 %, de 300 discos foliares, as estirpes fracas não foram detectadas pelos critérios adotados nessa investigação. Mais uma vez todas as plantas premunizadas e desafiadas apresentaram sintomas severos da doença, quatro meses após o desafio. A segunda hipótese foi estudada através de testes de proteção em plantas de crotalária premunizadas com as estirpes F-101 e F-144 e da análise da seqüência de nucleotídeos do gene da capa protéica das estirpes F-101, F-103 e PWV-SP. Nos testes de proteção, todas as plantas premunizadas com as estirpes fracas ficaram protegidas contra a infecção e/ou manifestação dos sintomas causados pela estirpe severa PWV-SP. Estudos quantitativos das estirpes fracas nessa hospedeira revelaram uma maior uniformidade na concentração do vírus nos tecidos foliares. A análise da seqüência de nucleotídeos do gene que codifica a capa protéica, apontaram identidade de 99,7 % entre as estirpes fracas e de 97,5 % destas com a estirpe severa, mostrando tratarem-se de estirpes do mesmo vírus. Esses resultados mostram que a premunização não parece ser uma alternativa adequada para o controle do endurecimento dos frutos do maracujazeiro, devido à falha na proteção. Essa quebra de proteção parece estar relacionada com a baixa concentração e/ou distribuição irregular das estirpes fracas nas folhas do maracujazeiro, que propiciam a existência de sítios de infecção para a estirpe severa posteriormente inoculada. / The main purpose of this work was to select mild strains of Passion fruit woodiness virus (PWV) and to evaluate their protective effect in passion flower (Passiflora edulis f. flavicarpa Deg.) challenged with a severe strain of the virus. Three mild strains were selected from outstanding plants found in orchards severely affected by the virus (F-101, F-102 and F-103) and three others were obtained from blisters formed in passion flower leaves with mosaic (F-99, F-144 and F-145). The protective effect of the mild strains was evaluated in passion flower under greenhouse and field conditions. Plants preimmunized with mild strains F-101, F-102 and F-144, under greenhouse conditions, showed partial protection after challenge inoculation with the severe strain PWV-SP. Total absence of protection was observed in passion flower preimmunized with all six mild strains and challenged with PWV-SP in the first field experiment. Due to these results, two hypotheses were raised to explain the intensification of symptoms in passion flower preimmunized with mild strains and challenged with the severe strain of the virus: a) the occurrence of low concentration and/or irregular distribution of the mild strains in the tissues of the preimmunized plants allow the infection and establishment of the later inoculated severe strain and b) the selected mild strains belong to a different species of Potyvirus, serologically related to PWV, but that do not offer protection against the severe strain of PWV. The first hypothesis was studied in a field experiment with passion flower preimmunized with mild strains F-101 and F-144, separately, and cultivated under screenhouse. Before the challenge inoculation, leaf samples were taken from five leaves of all protected plants and the concentration of the mild strains was estimated by indirect DAS-ELISA. A group of plants was challenged in three expanded leaves of the vine and another group was challenged with viruliferous aphids placed on the tip of the vine. All preimmunized plants showed severe symptoms of the disease, four months after the challenge inoculation. A great variation was observed in the concentration of the mild strains in the tissues of different leaves of the same plant. The ELISA test was not able to detect the mild strains in extracts of 205 out of 300 leaf disks. The second hypothesis was tested with crotalaria plants (Crotalaria juncea L.) preimmunized with mild strains F-101 and F-144 and analysis of the nucleotide sequence of the coat protein gene of the F-101, F-103 and PWV-SP strains. All preimmunized crotalaria plants were protected against the infection and/or manifestation of the symptoms caused by the severe strain PWV-SP. Quantitative studies of the mild strains in crotalaria revealed a larger uniformity in the concentration of the virus in the leaves. The analysis of the nucleotide sequence of the coat protein gene pointed out identity of 99.7% among the mild strains. The severe strain shared 97.5 % identity with both mild strains, showing that they are all strains of the same virus. These results showed that preimmunization does not seem to be an appropriate alternative for the control of the passion fruit woodiness disease in passion flower due to the breakdown in protection. Failure in protection seems to be related to the low concentration and/or irregular distribution of the mild strains in the leaves of the passion flower, which allow the occurrence of infection sites available for superinfection with the severe strain.

Passiflora edulis, popularly known in Brazil as sour passion fruit is the most widely cultivated species of the genus Passiflora, and is of economic importance in Brazil for industrial production of juice and fresh fruit for consumption. Despite its economic importance, little research has been conducted on this species, even on the most basic aspects. To fill in this gap, our group conducted various genetic studies, including estimating levels of molecular polymorphism, studying quantitative loci that control fruit yield and quality, and mapping genes conferring resistance to bacterial spot disease caused by Xanthomonas axonopodis. In addition, to enhance our knowledge of the P. edulis genome, a genomic library inserted into bacterial artificial chromosomes (BAC) has been constructed (82,944 clones, with coverage of 6× the species\' haploid genome). The library is kept at the French Plant Genomic Resources Center (CNRGV/INRA) in Toulouse. Initially, some 10,000 BES (BAC-end sequences) were sequenced, generating approximately 6.2 Mb of genomic information and providing an initial overview of P. edulis genome in terms of gene content and repeat sequences. With the aim of obtaining more comprehensive information, it was decided to select around 100 BAC inserts for complete sequencing. The aim of this study was to carry out genomic analysis in order to elucidate the structural and functional annotation of the genes, and identify and characterize transposable elements. The data generated by fully sequencing 10.4 Mb of P. edulis provide a rich source of information which has been taken full advantage of in this doctoral thesis. / Passiflora edulis, popularmente conhecido como maracujá azedo, é a espécie do gênero Passiflora mais cultivada, tendo importância econômica no Brasil tanto para a produção de suco industrializado quanto para o consumo da fruta fresca. Apesar da relevância da espécie, faltam pesquisas, principalmente nas áreas básicas. Para superar isso, nosso grupo tem realizado diversos estudos genéticos que incluem a estimativa dos níveis de polimorfismos moleculares, o estudo de locos quantitativos envolvidos no controle da produção e qualidade dos frutos e o mapeamento de genes de resistência à mancha bacteriana causada por Xanthomonas axonopodis. Além disso, para conhecer o genoma de P. edulis, foi construída uma biblioteca genômica inserida em BACs (82.944 clones, com cobertura de 6× do genoma haploide da espécie) que é mantida no CNRGV/INRA em Toulouse, França. Inicialmente, cerca de 10.000 BES (BAC-end sequences) foram sequenciadas, gerando aproximadamente 6,2 Mb de informação genômica, fornecendo a primeira visão do genoma de P. edulis sobre o conteúdo de genes e da porção repetitiva. Com o objetivo de obter informações mais completas, decidiu-se selecionar cerca de 100 insertos de BACs para o sequenciamento completo. A análise genômica realizada, especialmente a anotação estrutural e funcional dos genes e a identificação e caracterização dos elementos de transposição, constituíram os objetivos deste estudo. Os dados gerados pelo sequenciamento completo de 10.4 Mb de P. edulis representam uma fonte rica de informações que foram exploradas nesta tese de doutorado.

La thèse se propose de faire une incursion dans la pensée esthétique du fin XVIIe siècle-début XVIIIe, c’est-à-dire dans les débuts de cette nouvelle discipline en tant que science à part. Les contributions de Jean-Baptiste Dubos à la naissance de cette discipline sont massives, donc ce philosophe de l’art retient et mérite toute notre attention. D’abord, grâce à la Querelle des Anciens et des Modernes, son esprit s’enrichit et se forme, ses connaissances se multiplient de façon à lui permettre de réaliser une synthèse, dans son système d’esthétique, de la riche palette eidétique déployée jusqu’à lui. Ayant comme point de départ le cartésianisme, dont il se distingue entièrement, et dont il hérite uniquement le dogmatisme (l’universalité), cette fois-ci appliqué au sixième sens, celui qui juge de la valeur d’une œuvre artistique, il aboutit à s’ériger en auteur d’un courant opposé, apparenté au sensualisme. L’éloge des sens, au détriment de la raison, qui sont impliqués dans le jugement de goût du public invité à la diffusion de l’art, témoigne d’une coupure entre les époques ; la nouvelle ère appartient à Dubos et à ses disciples, qui répondent même des territoires étrangers, de l’Angleterre et même d’Allemagne. Les cinq parties de la thèse contiennent la description de la marche en avant, des pas de géant effectués dans le domaine des idées esthétiques par l’abbé Dubos. Si, dans la première partie, à l’occasion de la Querelle des Anciens et des Modernes, on découvre une rupture au sein des notions ; la notion de climat étant le noyau d’une théorie esthétique formulée avec le plus grand soin de détail par Dubos ; on découvre donc aussi une focalisation sur le sujet, créateur ou récepteur, siège du sentiment, la deuxième partie vient pour continuer à relever les mérites de Dubos, qui rend possible une attitude différente du public, public qui ne cherche plus l’instruction dans l’art, mais le plaisir, né de la satisfaction du besoin d’être occupé. Les trois autres parties explorent d’une façon plus profonde l’apport esthétique de Dubos : la critique psychologique, la critique scientifique et la critique comparée poésie/peinture. Les qualités de bon psychologue de Dubos, qui plonge dans l’âme du public au moment où il est attendri, rendent possible une meilleure création du génie, qui sait désormais s’adresser au public, de façon émouvante. À cet usage, Dubos formule une théorie du plaisir de l’art, du beau artistique et de l’émotion, qu’il incorpore de façon soudée à son système. Dans la quatrième partie, la critique scientifique, Dubos sonde l’âme du génie, le créateur surpris dans l’enthousiasme nécessaire à la naissance de l’œuvre d’art véritable, créée en vue de plaire. Lors du devenir du génie, les causes formatrices comme les causes morales ou les causes physiques se veulent responsables de ses productions artistiques, poésies ou peintures. De ces dernières nous nous sommes occupés dans la cinquième partie, en réalisant un parallèle, non seulement chez Dubos, mais aussi chez ses successeurs, comme Lessing, prioritairement. Une conclusion nous rappelle les mérites de l’abbé Dubos dans la fondation de l’esthétique moderne. / The thesis proposes to make a foray into the aesthetic thinking of late seventeenth century and early eighteenth century, that is to say into the beginnings of this new discipline as a science apart. Contributions from Jean-Baptiste Dubos to the birth of this discipline are massive, so the philosopher of art retains and is worthing all our attention. First, thanks to the Quarrel of the Ancients and the Moderns, his mind is enriched and formed, his knowledge grows in order to enable him to achieve a synthesis, in his aesthetic system, of the rich eidetic pallet deployed up to him. Having as a starting point the Cartesianism, from whom he differs entirely and from whom he inherits only dogmatism (universality), this time applied to the sixth sense, one who judges about the value of a work of art, Dubos arrives at setting himself up as a writer of an opposite current, akin to sensationalism. The praise of the senses, to the detriment of reason, senses involved in the judgment of taste of the public, invited to the dissemination of art, shows a break between periods ; the new era belongs to Dubos and his disciples, who answer, even from foreign territories, as England and even Germany. The five parts of the thesis contain the description of the evolution, of the gigantic steps forward made in the field of aesthetic ideas by Abbe Dubos. If, in the first part, thanks to the Quarrel of the Ancients and the Moderns, we find a break into the notions; the concept of climate being the nucleus of an aesthetic theory formulated with the utmost care of detail Dubos ; so also we find a focus on the subject, creator or receiver, subject who feels, the second part comes to continue to reveal the merits of Dubos, who makes possible a different attitude of public , public that no longer seeks for instruction in art, but for pleasure, born of a satisfied need, the one to be busy. The other three parts are exploring in a deeper way the aesthetic contribution of Dubos : psychological criticism, scientific criticism and comparative poetry / painting criticism. The qualities of Dubos as a good psychologist, who dips into the soul of the public when it is moved, make possible a better creation of genius, who now knows to speak to public, so moving. In this purpose, Dubos formulates a theory of the pleasure of art, artistic beauty and emotion, that he incorporates so welded to his system. In the fourth part, the scientific criticism, Dubos probes the soul of genius, the creator caught in the enthusiasm necessary for the birth of the true work of art, created to please. When the built of genius, formative causes as moral causes or physical causes are responsible for his artistic productions, poems or paintings. Both we have dealt with in the fifth part, by carrying out a parallel, not only in Dubos , but also among his successors, as Lessing priority . A conclusion reminds us of the merits of the Abbe Dubos in the foundation of modern aesthetics.

Ce mémoire traite du débat entre le coeur et le devoir et des différentes influences qui ont conduit à la fameuse question : Pourquoi la princesse n’épouse-t-elle pas le duc de Nemours alors que tous les obstacles sont levés ? À défaut de pouvoir répondre définitivement à cette énigme dont seule Madame de La Fayette possède la clé, nous nous sommes concentrée sur les influences qui auraient pu pousser la princesse à sacrifier son coeur pour son devoir. La méthode de recherche utilisée est l’approche de la biographie intellectuelle. Il est évident que le vécu de l'auteure a teinté le contenu de son oeuvre ainsi que l'âme de son héroïne. Nous nous sommes également inspirée de la méthode sociocritique quant aux influences et mouvements de l'époque. Dans le but de simplifier la collecte des sources et l’organisation de la recherche, nous avons regroupé les influences en trois grandes catégories : les influences personnelles, internes et externes. Cette étude a mis en évidence que les moeurs, dans La Princesse de Clèves, sont le miroir de la société du XVIIe siècle. Chaque influence présentée forme le dessin d’une princesse cachant en elle le secret de son renoncement à l'amour. / This work addresses the debate between heart and duty as well as the various influences that led to the famous question : Why doesn't the princess marry the Duke of Nemours when all barriers are removed ? All things considered, we cannot conclusively answer this enigma to which only Madame de La Fayette holds the key, therefore we focused on the influences that urged the princess to sacrifice her heart to her duty. The method of research used is the intellectual biography approach. It is apparent that the life of the author tinged the contents of her work and the soul of her heroine. We were also inspired by the sociological criticism method as to the influences and movements of this particular era. In order to simplify the collection and organization of this research, we combined the influences into three main categories : personal, internal and external. This study has shown that the characteristics in La Princesse de Clèves, mirror the society of the seventeenth century. Each presented influence forms the picture of a princess hiding, within herself, the secret of her renunciation of love.

Penser le rapport entre la politique, l’économie et la société civile de Hobbes à Mandeville, c’est traiter l’individu et les différentes transformations qu’il subit ; c’est-à-dire penser l’individu dans l’état naturel, dans l’état social et selon ses fonctions économiques. Chez Hobbes, pour arriver à un individualisme complet, il faut passer par un renoncement aux pouvoirs et aux facultés naturels de l’individu dans le cadre d’un état civil dans lequel le bien particulier s’accorde avec le bien commun; les biens des particuliers s’expriment en termes de propriété. Chez Mandeville le passage du naturel au social s’explique par le principe d’adaptation spontanée de l’individu à la nécessité de la production économique. Mandeville établit, contrairement à Hobbes, que l’harmonie des intérêts est involontaire et objective. D’où, l’homme économe de Mandeville est identifié par le travail ; pour Hobbes c’est le contrat qui permet de déterminer les premiers traits de l’individu économe. Dans ces deux ordres économiques différents, une théorie, que j’appelle « économico-individualiste », est ainsi nécessaire pour expliquer, comprendre et déterminer d’une part l’artificialisme de Hobbes, d’autre part, le hasard et la spontanéité de Mandeville. / Establishing the links between politics, economy and civil society from Hobbes to Mandeville is in fact studying the individual and the differents changes he undergoes. This means, analyzing the individual in he undergoes social state along with his economic functions. According to Hobbes , in order to reach complete individualism we need to go through the process of loosing powers and natural faculties of the individual within the framework of civil state in which the particular good fits the community properties; individual goods are manifested within the concept of property. Contrary to Hobbes, Mandeville states that the harmony of interests is involuntary and objective. Results from the unique interlacing of our national acts, according to Hobbes it’s the contract that determines the first features of the saving individual.

Este trabalho teve por objetivo selecionar estirpes fracas do Passion fruit woodiness virus (PWV) e avaliar o seu efeito protetor para o controle do endurecimento dos frutos do maracujazeiro. Foram selecionadas seis estirpes fracas do PWV. Três a partir de plantas de elite, encontradas em pomares severamente afetados pelo vírus (F-101, F-102 e F-103) e três a partir de bolhas formadas em folhas de maracujazeiro com mosaico (F-99, F-144 e F-145). O efeito protetor das estirpes fracas foi avaliado em maracujazeiros, em casa de vegetação e em campo. Em casa de vegetação foi observada uma proteção parcial das estirpes F-101, F-102 e F-144, contra a estirpe severa PWV-SP. Em campo, num primeiro experimento, as seis estirpes fracas selecionadas foram avaliadas e aproximadamente 4 meses após o desafio com a estirpe PWV-SP, todas as plantas apresentaram sintomas severos da doença. Diante da proteção parcial em casa de vegetação e da ausência total de proteção no experimento de campo, duas hipóteses foram apresentadas para explicar a intensificação de sintomas em maracujazeiros premunizados e desafiados com a estirpe severa do virus: a) a ocorrência de baixa concentração e/ou distribuição irregular das estirpes fracas nos tecidos das plantas premunizadas permite a infecção e estabelecimento da estirpe severa posteriormente inoculada e b) as estirpes fracas selecionadas são de uma espécie diferente de Potyvirus, serologicamente relacionada com o PWV, mas que não oferecem proteção contra a estirpe severa deste último. A primeira hipótese foi estudada repetindo-se o experimento com maracujazeiros premunizados com as estirpes F-101 e F-144, separadamente, e cultivados em campo sob condições de telado. Antes do desafio, foram feitos estudos quantitativos das estirpes F-101 e F-144, em diferentes folhas das plantas, através do DAS-ELISA indireto. Foi observada uma grande variação na concentração das estirpes fracas nos tecidos de diferentes folhas da mesma planta. Em 68,3 %, de 300 discos foliares, as estirpes fracas não foram detectadas pelos critérios adotados nessa investigação. Mais uma vez todas as plantas premunizadas e desafiadas apresentaram sintomas severos da doença, quatro meses após o desafio. A segunda hipótese foi estudada através de testes de proteção em plantas de crotalária premunizadas com as estirpes F-101 e F-144 e da análise da seqüência de nucleotídeos do gene da capa protéica das estirpes F-101, F-103 e PWV-SP. Nos testes de proteção, todas as plantas premunizadas com as estirpes fracas ficaram protegidas contra a infecção e/ou manifestação dos sintomas causados pela estirpe severa PWV-SP. Estudos quantitativos das estirpes fracas nessa hospedeira revelaram uma maior uniformidade na concentração do vírus nos tecidos foliares. A análise da seqüência de nucleotídeos do gene que codifica a capa protéica, apontaram identidade de 99,7 % entre as estirpes fracas e de 97,5 % destas com a estirpe severa, mostrando tratarem-se de estirpes do mesmo vírus. Esses resultados mostram que a premunização não parece ser uma alternativa adequada para o controle do endurecimento dos frutos do maracujazeiro, devido à falha na proteção. Essa quebra de proteção parece estar relacionada com a baixa concentração e/ou distribuição irregular das estirpes fracas nas folhas do maracujazeiro, que propiciam a existência de sítios de infecção para a estirpe severa posteriormente inoculada. / The main purpose of this work was to select mild strains of Passion fruit woodiness virus (PWV) and to evaluate their protective effect in passion flower (Passiflora edulis f. flavicarpa Deg.) challenged with a severe strain of the virus. Three mild strains were selected from outstanding plants found in orchards severely affected by the virus (F-101, F-102 and F-103) and three others were obtained from blisters formed in passion flower leaves with mosaic (F-99, F-144 and F-145). The protective effect of the mild strains was evaluated in passion flower under greenhouse and field conditions. Plants preimmunized with mild strains F-101, F-102 and F-144, under greenhouse conditions, showed partial protection after challenge inoculation with the severe strain PWV-SP. Total absence of protection was observed in passion flower preimmunized with all six mild strains and challenged with PWV-SP in the first field experiment. Due to these results, two hypotheses were raised to explain the intensification of symptoms in passion flower preimmunized with mild strains and challenged with the severe strain of the virus: a) the occurrence of low concentration and/or irregular distribution of the mild strains in the tissues of the preimmunized plants allow the infection and establishment of the later inoculated severe strain and b) the selected mild strains belong to a different species of Potyvirus, serologically related to PWV, but that do not offer protection against the severe strain of PWV. The first hypothesis was studied in a field experiment with passion flower preimmunized with mild strains F-101 and F-144, separately, and cultivated under screenhouse. Before the challenge inoculation, leaf samples were taken from five leaves of all protected plants and the concentration of the mild strains was estimated by indirect DAS-ELISA. A group of plants was challenged in three expanded leaves of the vine and another group was challenged with viruliferous aphids placed on the tip of the vine. All preimmunized plants showed severe symptoms of the disease, four months after the challenge inoculation. A great variation was observed in the concentration of the mild strains in the tissues of different leaves of the same plant. The ELISA test was not able to detect the mild strains in extracts of 205 out of 300 leaf disks. The second hypothesis was tested with crotalaria plants (Crotalaria juncea L.) preimmunized with mild strains F-101 and F-144 and analysis of the nucleotide sequence of the coat protein gene of the F-101, F-103 and PWV-SP strains. All preimmunized crotalaria plants were protected against the infection and/or manifestation of the symptoms caused by the severe strain PWV-SP. Quantitative studies of the mild strains in crotalaria revealed a larger uniformity in the concentration of the virus in the leaves. The analysis of the nucleotide sequence of the coat protein gene pointed out identity of 99.7% among the mild strains. The severe strain shared 97.5 % identity with both mild strains, showing that they are all strains of the same virus. These results showed that preimmunization does not seem to be an appropriate alternative for the control of the passion fruit woodiness disease in passion flower due to the breakdown in protection. Failure in protection seems to be related to the low concentration and/or irregular distribution of the mild strains in the leaves of the passion flower, which allow the occurrence of infection sites available for superinfection with the severe strain.

Le XVIIIe siècle n’est pas seulement le siècle de la raison, il est aussi celui où le sentiment s’impose dans l’esprit de certains philosophes pour rendre compte de la façon dont certains objets sont connus. Le moi ainsi que les valeurs morales et esthétiques sont, par excellence, des objets qui échappent à une analyse rationnelle ainsi qu’aux perceptions issues des sens externes. Ils se donnent dans cette expérience intérieure qu’est le sentiment. La particularité de celui-ci est que, alors qu’il est une impression d’ordre affectif, constituée de perceptions de plaisir et de douleur, il est amené à représenter autre chose que l’état purement subjectif de l’âme. Tout le problème est de déterminer à quel point le sentiment constitue un mode de connaissance irréductible : est-il un principe de connaissance à part entière, à côté de la sensation et de la réflexion, ou simplement la manière de connaître de celui qui, ayant développé des habitudes de penser et de sentir, a l’impression de juger de façon immédiate ? Reconnu comme fait mais n’ayant pas de fondement clairement assignable, il est sujet aux interprétations les plus contradictoires. Placé au croisement d’un courant métaphysique et d’un courant empiriste radical, il incarne une des notions qui manifestent le plus fortement la diversité des écoles qui perdurent au siècle des Lumières. / The 18th century is not only the age of reason, it is also the time when the sentiment becomes very important in the mind of some philosophers to explain how a certain kind of objects are known. The self as well as the moral and esthetic values are, par excellence, objects that escape both the rational analysis and the perceptions derived from external senses. They are given in an internal experience called sentiment, whom particularity is to represent something different from the pure subjective state of mind, although it is an affective impression, made of perceptions of delight and pain. The problem is to determine in what extent the sentiment represent an irreducible way of knowing: is it a source of knowledge of its own, next to sensation and reflection, or is it just an impression one’s get of judging immediately which occults a succession of unconscious judgments? Acknowledged as a fact, but lacking obvious foundation, it is likely to receive the most contradictory interpretations. At the intersection of a metaphysical current and an empiricist one, it embodies one of the notions that exhibit the diversity of schools which remains in the Enlightenment.

À La Réunion, les activités de pleine nature sont au cœur d'enjeux environnementaux importants. De plus en plus, ces activités sont présentées comme des pratiques passionnées. Selon le type de passion, l'engagement dans l'activité est différent et le degré d'ouverture à la nature et à l'environnement pourrait être impacté. En ce sens, la passion pourrait être un facteur clé dans le développement d'un pro-environnementalisme à la suite de la pratique de ces activités. L'objectif de cette thèse est d'étudier le rôle de la passion pour une activité de pleine nature sur l'affiliation à la nature et le conflit vis-à-vis de la protection de l'environnement et leur influence sur la motivation environnementale, tout en soulignant le rôle des émotions et de l'attachement au lieu comme médiateurs. / In La Réunion, outdoor activities are at the core of environmental issues. Increasingly, these activities are presented as passionate practice. According to the kind of passion, engagement in the activity is different and the degree of opening to nature and environment would be impacted. Therefore, passion could be a key factor in pro-environmentalism development following outdoor activities practice. The objective of this thesis is to study the role of passion for outdoor activity on nature affiliation and conflict toward environmental protection and their influence on environmental motivation, while emphasizing the role of emotions and place attachment as mediators.

The phenomenon of crunch is well established in the computer game industry, but is still unfamiliar in other areas. Crunch represents a period in the work phase that is characterized by a high and intense workload in connection with deadlines and upcoming game releases that exist in order to speed up a project. This period is most often characterized by being unpaid (Cote & Harris, 2020, p. 161; Cote & Harris, 2021, p. 1). The purpose of this study is to broaden and deepen the understanding of the phenomenon of crunch in the computer game industry from the perspective of computer game developers. The focus of the thesis will be on answering why crunch still exists in the computer game industry, what drives computer game developers to perform a crunch and what effects crunch has on computer game developers. The study's theoretical frame of reference presents five different themes that form the basis for the study's results: passion, motivation, working conditions, culture and work-life balance. A qualitative approach is the chosen method for this study where the empirical material has been collected through eight digital interviews with computer game developers. All interviews were recorded with the approval of the respondents and subsequently transcribed. Furthermore, a thematic analysis was performed on the collected material. The conclusions of the study are that crunch still exists in the computer game industry due to the computer game developers long-standing passion for work, prevailing working conditions and the maintenance of a crunch culture where computer game developers give in to informal demands. Furthermore, there are different factors that drive a computer game developer to carry out a crunch, of which social connectedness, creativity, self-realization, autonomy, competence, personal development, honor and fame are driving forces. The emotions that computer game development generates as well as goal completion are also contributing factors to what drives computer game developers to carry out a crunch. Finally, crunch can lead to many different effects on computer game developers, ranging from physical and mental effects to lack of productivity and creativity, where one’s private life may be affected. The effects of crunch presented in the study are of a negative nature which impacts the health and well-being of computer game developers. Concluding the study is a discussion of the strengths and weaknesses with the thesis including suggestions for future research. / Inom dataspelsindustrin är fenomenet crunch väletablerat, men utanför är begreppet fortfarande främmande. Crunch representerar en period i arbetsfasen som kännetecknas av hög och intensiv arbetsbelastning i samband med deadlines och kommande spelsläpp som existerar i syfte för att skynda på ett projekt. Denna period kännetecknas oftast av att vara obetald (Cote & Harris, 2020, s. 161; Cote & Harris, 2021, s. 1). Syftet med studien är att bredda och fördjupa förståelsen om fenomenet crunch inom dataspelsindustrin utifrån dataspelsutvecklares perspektiv. Fokus i uppsatsen kommer att ligga på att besvara varför crunch fortfarande existerar inom dataspelsindustrin, vad som driver dataspelsutvecklare att genomföra en crunch samt vad crunch har för effekter på dataspelsutvecklare.  Studiens teoretiska referensram presenterar fem olika teman som ligger till grund för studiens resultat. De fem teman som behandlas i studien är passion, motivation, arbetsförutsättningar, kultur och Work-life balance. Den valda metoden för studien är en kvalitativ ansats där det empiriska materialet har samlats in via åtta digitala intervjuer med dataspelsutvecklare. Samtliga intervjuer har spelats in med godkännande av respondenterna och därefter transkriberats. Vidare så utfördes en tematisk analys på det insamlade materialet.  Slutsatserna i studien är att crunch fortfarande existerar inom dataspelsindustrin på grund av dataspelsutvecklarnas långvariga passion till arbetet, rådande arbetsförutsättningar och upprätthållandet av en crunch-kultur där dataspelsutvecklare ger vika för informella krav. Vidare finns det flera olika faktorer som driver en dataspelsutvecklare att genomföra en crunch, varav den sociala tillhörigheten, kreativitet, självförverkligande, autonomi, kompetens, personlig utveckling, ära och berömmelse är drivkrafter. Även de känslor som dataspelsutvecklandet genererar och tillfredsställelsen av att uppnå ett mål är bidragande faktorer till vad som driver en dataspelsutvecklare till att genomföra en crunch. Slutligen kan crunch leda till många olika effekter på dataspelsutvecklare, allt från fysiska och psykiska effekter till bristande produktivitet och kreativitet, där även den privata sfären drabbas. Effekterna av crunch som presenterats i studien har varit av negativ karaktär och haft inverkan på dataspelutvecklares hälsa och välbefinnande. Studien avslutas med styrkor av svagheter med uppsatsen samt förslag på framtida forskning.