Diversas proteínas pneumocócicas têm sido estudadas como candidatos vacinais. Entre elas, PspA e Ply induzem anticorpos essenciais para a proteção contra sepse, enquanto, SP 0148 e SP 2108 induzem IL-17 e protegem camundongos contra a colonização. Esse trabalho teve como objetivo principal desenvolver vacinas pneumocócicas baseadas em proteínas. Primeiramente, foi selecionada uma molécula de PspA com ampla reatividade cruzada. Em seguida, esta PspA foi fusionada com PdT, um pneumolisóide derivado da Ply. Essa proteína de fusão mostrou-se capaz de induzir resposta imunológica humoral e celular e protegeu camundongos contra desafio letal. Vacinas baseadas em BCG, que possui diversas propriedades adjuvantes, foram desenvolvidas expressando as proteínas pneumocócicas rPspA-PdT, SP 0148 e SP 2108. A imunização com o rBCG 0148/rSP 0148 induziu IL-17 e levou a proteção contra colonização. A combinação das três vacinas de rBCG mostrou-se mais eficiente na proteção contra desafio de colonização. Esses resultados sugerem um uso promissor do rBCG como vacina pneumocócica. / Several pneumococcal proteins have been proposed as vaccine candidates. PspA and Ply induce protective antibodies against sepse, while SP 0148 and SP 2108, induce IL-17 and protect mice against pneumococcal colonization. The major aim of this study was to produce pneumococcal vaccines based on proteins. First, we selected one PspA molecule able to induce broad-ranging cross-reactivity. Second, we constructed a hybrid protein containing a PspA fused to PdT, a detoxified form of Ply. The hybrid protein was able to induce humoral and cellular responses and protected mice against lethal challenge. Finally, due the adjuvant properties of BCG, we constructed recombinant BCG strains expressing PspA-PdT, SP 0148 and SP 2108. The immunization with rBCG-0148/rSP 0148 induced IL-17 and IFN-, and pneumococcal colonization in mice. Interestingly, the combination of all rBCG vaccines was more efficient in protecting mice against pneumococcal colonization. These results suggesting a promising use of rBCG as pneumococcal vaccine.

Inledning: Även om distansterapi visat sig vara effektivt och ger ökad tillgänglighet är många terapeuter ändå tveksamma till formatet. Syfte: Genom att undersöka hur övergången till distansformat enligt psykodynamiskt orienterade terapeuter påverkar den terapeutiska processen, dess ram och relationella kvalité ges ett bidrag till förståelse av denna tvekan. Metod: En kvalitativt inriktad fenomenologisk studie av semistrukturerade intervjuer med fem terapeuter. Resultat: Tematisk analys visar två teman: Närvaro och En ny situation med två respektive fyra underteman: möjliggörande och saknad respektive kropparnas kommunikation, saknad avgränsning, ramarbete och inre upptagenhet. Diskussion: En inre upptagenhet kan göra att närvaro som relationell kvalité i bemärkelsen emotionell, kognitiv och funktionell tillgänglighet försvåras i distansformatet. Inre upptagenhet kan bero på de ramfrågor som aktualiseras i övergången. Undersökningen resulterar i en modell som visar att i den mån en viss terapeutisk teknik, känsla i rummet eller terapeutroll föreligger som målsättning i den terapeutiska processen kommer den inre upptagenheten sannolikt kvarstå. Ökad förståelse för terapeuters målsättningar i och med den terapeutiska processen föreslås som en nyckel för vidare acceptans av distansformatet. / Introduction: Even though distance therapy has been shown to be effective across a range of measures and increases access many psychotherapists still remain sceptic to the format.   Aim: By examining how psychodynamic psychotherapists experience transitioning to distance therapy as regards the therapeutic process this study results in a contribution to the understanding of the continuing scepticism among therapists.  Method: A qualitative, phenomenological study of semi-structured interviews with five psychotherapists using thematic analysis.  Result: Two themes are identified: Presence and A new situation with two and four subthemes respectively: enabling and lamented; bodily communication, demarcation, therapeutic frame and preoccupation. Discussion: Due to preoccupation presence understood as a relational quality of emotional, cognitive and functional availability could be continually lamented in distance therapy. The discussion results in a model showing that if a certain therapeutic technique, feeling in the room or therapeutic role is an underlying intention in the therapeutic process preoccupation will continue to be a factor in distance therapy encounters. Increased knowledge as regards therapists´ intentions in the therapeutic process is forwarded as a key factor to further acceptance of the distance therapy format.

Die Photodynamische Therapie (PDT) hat sich zunehmend als vielversprechende Methode zur Behandlung von verschiedenen malignen Neubildungen gezeigt. Die photodynamische Zerstörung der Tumore wird erreicht indem zunächst ein Photosensibilisator entweder lokal oder systemisch appliziert wird und im Anschluss an eine gewisse Inkubationszeit die Tumormasse mittels einer Lichtquelle mit einer spezifischen Wellenlänge durchleuchtet wird. Aufgrund der bevorzugten Anreicherung des Photosensibilisators in Tumorzellen, erlaubt diese Methode eine selektive Abtötung des malignen Tumors, während das umliegende Gewebe weitestgehend verschont wird. Diese Eigenschaften und Anforderungen machen die PDT, insbesondere in den Fällen, wo die chirurgische Enukleation als kurative Option erwogen wird, zu einer attraktiven Therapieoption in der Behandlung von Retinoblastomen (Rb). Die extreme Methode der Enukleation wird noch immer angewendet, wenn die Tumoren nicht ausreichend chemosensibel sind, oder wenn sich die Erkrankung aufgrund von unzureichendem Zugang zu medizinischer Versorgung bereits in einem fortgeschrittenen Stadium befindet. In dieser Studie haben wir zunächst In-Vitro-Untersuchungen mit dem neuen kationischen wasserlöslichen Photosensibilisator Tetrahydroporphyrin-Tetratosylat (THPTS) bezüglich seiner photodynamischen Wirkung auf WERI Rb-1 und Y79-Retinoblastomzellen durchgeführt. Dabei konnten wir zeigen, dass weder die Inkubation mit THPTS ohne anschließende Beleuchtung, noch die alleinige Beleuchtung zu einem signifikanten Effekt auf die Proliferation der Rb-Zellen führte. Die Kombination von THPTS mit anschließender Beleuchtung hingegen führte zu einem maximal zytotoxischen Effekt in den Tumorzellen. Darüber hinaus war die Phototoxizität in normalen Primärzellen des Pigmentepithels der Retina geringer, wodurch ein erhöhter phototoxischer Effekt von THPTS in Krebszellen gegenüber diesem normalen Zelltyp der Retina gezeigt werden konnte. Die vorliegenden Ergebnisse bilden eine ermutigende Grundlage für weiterführende in-vivo-Untersuchungen zum therapeutischen Potential dieses vielversprechenden Photosensibilisators mit der Aussicht auf eine potentiell kurative Therapie des Retinoblastoms unter Erhalt von Augapfel und Visus.:Inhaltsverzeichnis Einleitung 1 Hintergrund und Bedeutung 1 Pathophysiologie 2 Diagnostik und Symptome 3 Klassifizierung 4 Therapie und Prognose 5 Enukleation 5 Perkutane Radiotherapie 6 Brachytherapie 6 Intravenöse Chemotherapie 6 Intraarterielle Chemotherapie 6 Laser-gestützte Verfahren 7 Photodynamische Therapie 7 Mechanismus 7 Eigenschaften von Photosensibilisatoren 8 THPTS-PDT 9 Fragestellung 9 THPTS-PDT on Human Retinoblastoma Cell Lines 11 Zusammenfassung 24 Einleitung 24 Methode 25 Zellkultur primärer humaner Pigmentepithelzellen der Retina (RPE) 25 Photodynamische Therapie von Retinoblastomzellen und RPE-Zellen 25 Clearance von THPTS 25 Real-Time RT-PCR 25 Immunzytochemie und Live-Videoaufnahmen 26 Ergebnisse 26 Effekt der THPTS-PDT auf Retinoblastom-Zelllinien 26 Effekt in Abhängigkeit von Dosis und Einwirkzeit 26 Wirkung der THPTS-PDT auf nicht-maligne Netzhautzellen im Vergleich zu Rb-Zellen 27 Verstoffwechselung von THPTS 27 Genexpression 27 Immunzytochemie und Live-Videoaufnahmen 28 Subzelluläre Anreicherung von THPTS 28 Diskussion 28 ii Literaturverzeichnis vi Anhang x Erklärung über die eigenständige Abfassung der Arbeit x Wissenschaftliche Publikationen xi

This student thesis in General Management addresses how leaders can bridge the gap between work groups and teams in geographically dispersed organizations and partially distributed teams. These types of organizational structures are increasingly common in the globalized world of business, and bring benefits to many organizations by for example connecting skilled workers regardless of their location through the means of information and communications technology. However, previous research within the field of work in dispersed settings has identified several challenges that these settings entail, including areas like for example group cohesion and motivation. If not handled, these challenges may have negative effects on team performance and organizational effectiveness. Previous studies have mostly targeted the challenges in isolation. The purpose of this study is to provide a holistic perspective, connecting different challenges in order to pinpoint reasons and effects. By identifying consequences that follow from being geographically dispersed and investigating how the challenges affect a real-world organization, the study aims to suggest countermeasures to deal with these consequences. Theory is built using Informed Grounded Theory, based on primary data from 21 in-depth interviews conducted at a Swedish high tech company. Through an analysis combining the primary data with secondary data stemming from relevant literature, the study presents conclusions including suggested countermeasures to overcome challenges imposed by work in dispersed settings. The study identifies communication as the key factor with possibility to affect group cohesion and motivation directly, and thereby also performance indirectly. Thoughtful use of different types of communication can in fact counteract challenges and lead to increased productivity and well-being. The study has implications for organizations that are planning for, or currently utilizing a dispersed organizational structure, and aids in understanding the collected effects of the challenges involved. The study is conducted at one company, which can be seen as a limitation. To counteract for this limitation, the researchers have put in effort to emphasize generalizable factors.

Submitted by PPG Ci?ncias Sociais (csociais-pg@pucrs.br) on 2018-10-25T19:12:11Z No. of bitstreams: 1 M?rcio_Soares_ Rodrigues_Dis.pdf: 1650077 bytes, checksum: f295f0bb4fc140f92ad684e3b5956a9d (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-10-26T13:46:49Z (GMT) No. of bitstreams: 1 M?rcio_Soares_ Rodrigues_Dis.pdf: 1650077 bytes, checksum: f295f0bb4fc140f92ad684e3b5956a9d (MD5) / Made available in DSpace on 2018-10-26T13:52:24Z (GMT). No. of bitstreams: 1 M?rcio_Soares_ Rodrigues_Dis.pdf: 1650077 bytes, checksum: f295f0bb4fc140f92ad684e3b5956a9d (MD5) Previous issue date: 2018-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The main purpose of this research is to answer the following question: what were the factors that influenced the members of the MDB party to choose between PMDB or PDT when the multiparty system was restored in the late 1970? We intended to answer that question in two parts. First, we will do a historical analysis that goes from the multiparty system period - that began after the ?Estado Novo? regime - until the final moments of the bipartisanship system, in order to, as proposed by Panebianco (2005), prioritize the analysis of the foundation of the political parties, especially the sections of PTB and MDB from Rio Grande do Sul, as well as its development in such an unusual context as an opposing party, seeking to know how power were distributed in both organizations and the consequences of this interaction when the MDB party was extinguished. Then, we will analyze the political history of the MDB members from the elite of Rio Grande do Sul, searching for patterns in their political careers that may elucidate the choices between the two parties, highlighting the MDB leaders that have decided to join PMDB or PDT. Based on the journalistic coverage of the newspapers ?Zero Hora? and ?Folha da Tarde? from that period of time, we will also investigate how those leaders publicly justified their decision, in order to, considering the Panebianco (2005) study of the collective and selective incentives, verify if they use ideological and/or doctrinal arguments to explain their decisions, according to the narrative structured by the main leaders of those two political parties in that time: Leonel Brizola and Pedro Simon. / A presente disserta??o tem como objetivo principal responder ? seguinte quest?o: quais fatores colaboraram para a defini??o dos emedebistas ga?chos pelo PMDB ou PDT quando do retorno ao pluripartidarismo no final da d?cada de 1970? Buscamos responder das seguintes formas. Primeiro, faremos uma an?lise hist?rica do per?odo pluripartid?rio que se inicia ap?s o Estado Novo at? os momentos finais do bipartidarismo, visando assim, conforme proposto por Panebianco (2005), priorizar a an?lise do momento de nascimento das legendas, principalmente as se??es ga?chas do PTB e do MDB, bem como seu desenvolvimento em um contexto t?o at?pico para uma legenda oposicionista, buscando conhecer a estrutura e forma como o poder era distribu?do dentro de ambas ?s organiza??es, assim como a consequ?ncia desta intera??o no momento da extin??o do MDB. Segundo, atrav?s da an?lise da trajet?ria pol?tica da elite emedebista do Rio Grande do Sul, buscaremos encontrar padr?es de carreiras pol?ticas que possam elucidar a escolha da agremia??o a ser adotada com o retorno do pluripartidarismo, priorizando assim aqueles l?deres emedebistas que caminham para o PMDB ou o PDT. Com base na cobertura jornal?stica dos jornais Zero Hora e Folha da Tarde do per?odo, vamos analisar ainda como os l?deres emedebistas justificaram publicamente suas decis?es a respeito dos partidos adotados com a extin??o do MDB, visando assim, com base nos incentivos coletivos e seletivos de Panebianco (2005), verificar se estes utilizam de argumentos ideol?gicos e/ou doutrin?rios para explicar suas decis?es, conforme a narrativa constru?da pelos principais l?deres das duas legendas no per?odo, Leonel Brizola e Pedro Simon.

La thérapie photodynamique (PDT) est une modalité de traitement des petites tumeurs localisées accessibles à la lumière. Son principe repose sur l'action conjuguée d'un photosensibilisant, de la lumière et de l'oxygène.<br />Une dosimétrie correcte est nécessaire pour assurer le traitement complet et des résultats reproductibles. Un élément clé de la dosimétrie à prendre en compte est le photoblanchiment, étant donné qu'il diminue la concentration du photosensibilisant au cours du traitement. Il est donc indispensable d'appréhender les mécanismes du photoblanchiment pour maîtriser au mieux le traitement.<br />Le rendement quantique de photoblanchiment de la m-THPBC en solution avec des protéines est de 5.7 x 10-4, les formes agrégées de photosensibilisants photoblanchissent plus lentement que les formes monomérisées. La m-THPBC se transforme sous l'effet de la lumière en m-THPC, ce rendement de phototransformation est influencé par l'état d'agrégation de la molécule. D'autres photoproduits tels que la m-THPBC di-hydroxylée et la m-THPC dihydroxylée ainsi que des dérivés dipyrines ont été observés. L'espèce responsable du photoblanchiment a été identifiée comme étant l'oxygène singulet. In vitro, la LD50 de la m-THPBC est 0.7 J cm-2. Le photoblanchiment est 3 à 5 fois plus sensible que celui de la m-THPC. L'étude de la localisation intra-cellulaire des photosensibilisants a montré une différence, la m-THPC s'accumulant dans le réticulum endoplasmique alors que la m-THPBC se localise dans les mitochondries. In vivo, la m-THPBC possède un photoblanchiment 4 à 10 fois plus élevé que la m-THPC, ainsi qu'une bonne accumulation tumorale. Ces résultats in vitro et in vivo sont particulièrement intéressants en terme de ratio thérapeutique, de sélectivité du traitement et de photosensibilité cutanée.

In the integrated public affairs management framework, except information communication, mass communication media also have economic industrial characteristics. Issues from mass communication media must make people have clinical and neutral cognition to avoided influences on people¡¦s judgements about facts. According to the study of the third-person effect, we proved that people mentually exist cognitive biases against communication information. Thus they always become operated subjects of elections. In order to understand subjects¡¦ cognitive and judge models, we employed three major factors, two situations of negative and positive news about THSR (Taiwan High-Speed Railway) to design our Information Integration Theory research. We also compared with the south and north people in Taiwan to show unbalances of the regional development about mass media and population quality. Findings of the research are as follow¡G 1. The north people with different issue involvements, have more movements in the the third-person effect in the negative news situation. 2. In the negative news situation, the north people¡¦s third-person effect and information reliability were represented by a negative correlation. 3. Media expose and people¡¦s third-person effect were represented by a positive correlation. 4. People¡¦s information integration models were following the adding rule. 5. The north people have more intention to support media regulation with the public opinion and actions. At last, we concluded several suggestions: 1. Unbalances of the regional development in Taiwan not only between the south and north, we must invest more resources to study and record. 2. Our study was a tiny subsection of public affairs management framework, researchers could study deeper and wider in the future. 3. The statistic tool of Information Integration Theory need to be updated to simplify importing data.

<p>Under de senaste åren har olika former av kognitiv distraktion, till exempel att tala i mobiltelefon med handsfree, relaterats till en betydande mängd olyckor i trafiken. Av den anledning finns det ett stort intresse i att ta fram olika tekniska system för att upptäcka förare som är distraherade. Målet med denna studie var att ta ett steg tillbaka och undersöka människors förmåga och metoder för att avgöra distraktion hos andra. Då det saknas tidigare forskning är upplägget en explorativ förstudie bestående av två delar. Under den första delen samlades material in på distraherade bilförare i simulerad förarmiljö. Distraktionen bestod av olika uppgifter designade för att skapa kognitiv distraktion. Förarnas arbetsbelastning mättes med Peripheral Detection Task (PDT). Resultaten visar att de mer komplexa kognitiva uppgifterna försämrar prestationen på PDT, men ingen skillnad dem sinsemellan observerades. I den andra delen testades hur ett antal bedömares uppskattning av distraktion hos förarna stämde överens med prestationen på PDT. Resultaten kan inte påvisa en förmåga att upptäcka distraktion hos andra, men ett antal svagheter i metoden diskuteras vilket kan ge vägledning för metoddesign i framtida studier.</p>

Made available in DSpace on 2016-08-17T18:39:47Z (GMT). No. of bitstreams: 1 5345.pdf: 1839849 bytes, checksum: 117cb76c1a10deab4b3e8c9df4d797f3 (MD5) Previous issue date: 2012-03-30 / In Brazil there was an increase of post-surgical infections by rapidly growing mycobacteria, mainly Mycobacterium massiliense, leading the Agency for Sanitary Surveillance (ANVISA) to establish standards for the mandatory notification of cases occurring nationwide. In order to deal with a major public health problem, this study aimed to determine the efficiency of using antimicrobial photodynamic inactivation (PDIa) in Mycobacterium massiliense for future use in the treatment of agent infections. The objective was to test in vitro technique the PDI to control Mycobacterium massiliense to determine the best light dose and concentration of each photosensitizer (FS). To accomplish this search were used two FS: Photogem® and salt Curcumin (N-Methyl-D-Glucominato of Curcumin, 31.8%). As a light source for radiating a BioTable ® operated at 630nm (red light) for Photogem ® and 460nm (blue light) to the salt of curcumin. After the standardized inoculum was added to the sample analyzed in triplicate to a 24 well plate which contained 500&#956;l aliquots of the inoculum +500 &#956; l of the FS for the study groups tested, and the control group containing 1 ml of inoculum, and five minutes as time incubation (protected from light). After expiration of the irradiation time in accordance with the previously analyzed fluences of the tested groups and subsequent dilution of the sample to the value of 10-5, 100mL sample were plated in triplicate on 7H10 agar + OADC 10% and maintained at 37 for about 5 days with subsequent counting of colony forming units. Based on the results, they showed that FS Photogem ® until the concentration of 1000 &#956;g/Ml was not effective in reducing the number of CFU of M. massiliense. In contrast, the FS Salt Curcumin was effective at concentrations of 1300 &#956;g/Ml with a light dose 50 J/cm2 compared to controls. Thus, these data suggest that the salt FS Curcumin in photodynamic action is a viable alternative for reducing M massiliense when tested in vitro. / No Brasil houve um crescimento de infecções pós-cirúrgicas por micobactérias de crescimento rápido, principalmente por Mycobacterium massiliense, levando a Agência de Vigilância Sanitária (ANVISA) estabelecer normas de notificação obrigatória dos casos ocorridos em todo território nacional. Tendo em vista de se tratar de um grande problema de saúde pública, o presente trabalho visou determinar a eficiência da atividade antimicrobiana utilizando Inativação Fotodinâmica (IFD) em Mycobacterium massiliense para futura utilização no tratamento de infecções por esse agente. O objetivo foi testar in vitro, a técnica da IFD no controle de Mycobacterium massiliense, para determinar a melhor dose de luz e concentração de cada fotossensibilizador (FS). Para realizar esta pesquisa foram utilizados dois FS: Photogem® e Sal de Curcumina (N-Metil-D-Glucominato de Curcumina, a 31,8%). Como fonte de luz para irradiação, uma BioTable® operou em 630nm (luz vermelha) para o Photogem® e em 460nm (luz azul) para o Sal de Curcumina. Após padronizado o inóculo, a amostra analisada foi adicionada em triplicata a uma placa de 24 poços a qual continha alíquotas de 500&#956;l do inoculo +500&#956;l do FS para os grupos de estudos testados, e o grupo controle contendo 1ml do inoculo, tendo 5 minutos como tempo de incubação (protegido da luz). Após decorrido o tempo de irradiação de acordo com as fluências previamente analisadas dos grupos testados e com posterior diluição da amostra até o valor de 10-5, 100&#956;l da amostra foram semeadas em triplicada em placas contendo Agar 7H10 + OADC 10% e mantidas a 37o por aproximadamente 5 dias com posterior contagem das unidades formadoras de colônias. Com base nos resultados obtidos, estes mostraram que o FS Photogem® até a concentração de 1000&#956;g/ml não foi efetivo na redução do número de UFC de M. massiliense. Em contraponto, o FS Sal de Curcumina foi efetivo a partir da concentração de 1300 &#956;g/ml com dose de luz a 50 J/cm2 comparado ao grupo controle. Assim, estes dados sugerem que o FS Sal de Curcumina sob ação fotodinâmica é uma alternativa viável para a redução de M. massiliense quando testadas in vitro.

Syftet med denna uppsats var att undersöka behandlingseffekten av studentledd psykoterapi på Umeå Universitets psykologmottagning gällande depressions- och ångestsymptom. Mer specifikt var syftet även att jämföra de två behandlingsmetoder som praktiseras på mottagningen; kognitiv beteendeterapi (KBT) och psykodynamisk terapi (PDT), samt jämföra de självskattningsinstrument för depression och ångest som ingår i mottagningens testbatteri (Patient Health Questionnaire 9-items, PHQ-9; Hospital Anxiety and Depression scale, HADS; General Anxiety Disorder 7-item scale, GAD-7). Ett ytterligare delsyfte var att beskriva mottagningens klientpopulation. Stickprovet bestod av 61 deltagare (72.1 procent kvinnor, 49.2 procent studenter, medianålder 23 år) som behandlats med antingen KBT eller PDT. För att undersöka effekterna av behandling jämfördes deltagarnas symptomskattningar före och efter behandling uppdelat på behandlingsmetod med hjälp av två-vägs variansanalys (ANOVA). Resultaten visade stora effektstorlekar för behandling gällande depression och ångest. Inga signifikanta skillnader observerades mellan behandlingsmetoder, vilket kan bero på bristande power. Diskrepans mellan instrumentens förmåga att identifiera deltagare med symptom på depression och ångest kunde ses, i synnerhet gällande depressionssymptom. I linje med rådande forskning visar studiens resultat att studentledd behandling har effekt på depression och ångest. Validiteten för de instrument som används på Psykologmottagningen bör dock undersökas närmare. Sammantaget belyser resultaten i denna studie vikten av att välja mätinstrument anpassade för den tilltänkta populationen för att kunna utvärdera behandlingseffekt.